Filters
total: 1486
-
Catalog
- Publications 1134 available results
- Journals 9 available results
- Conferences 1 available results
- Publishing Houses 18 available results
- People 77 available results
- Inventions 4 available results
- Projects 82 available results
- Laboratories 4 available results
- Research Teams 1 available results
- Research Equipment 7 available results
- e-Learning Courses 19 available results
- Events 30 available results
- Open Research Data 100 available results
displaying 1000 best results Help
Search results for: SPH
-
Myrosinase activity in different plant samples; optimisation of measurement conditions for spectrophotometric and pH-stat methods
PublicationMyrosinase found in Brassicaceae plants, is the enzyme responsible for hydrolysis of glucosinolates. As a result a variety of biologically active metabolites are liberated, whose importance in crop protection and especially in cancer chemoprevention is rapidly gaining recognition. The growing practical application of glucosinolate degradation products requires that sensitive and reliable methods of myrosinase activity determination...
-
Studies on Treatment of Bitumen Effluents by Means of Advanced Oxidation Processes (AOPs) in Basic pH Conditions
PublicationThe paper presents the results of studies on chemical treatment of effluents from production of bitumen of petroleum origin. Due to the presence of sulfide ions, the pH of these effluents is strongly alkaline. Several Advanced Oxidation Processes (AOPs) were studied, including the use of hydroxyl and sulfate radicals oxidants, the hydrodynamic cavitation as well as sonocavitation. The best processes allow to obtain 45% reduction...
-
Projekt dwóch belek hybrydowych dla firmy H+H Polska sp. z o.o.
PublicationOpracowanie stanowi projekt dwóch belek nadprożowych o długości 3,0 m każda o przekroju poprzecznym belki hybrydowej (beton konstrukcyjny łączony z betonem komórkowym) nawiązującej do wymiaru bloczka z betonu komórkowego firmy H+H Polska sp. z o.o. o wymiarach (szerokość × wysokość × długość): 24 × 24 × 60 cm, odmiany D600 o średniej wytrzymałości na ściskanie 3,0 MPa (zgodnie z kartą techniczną w załączniku do opracowania). Całkowita...
-
Studies on treatment of bitumen effluents by means of Advanced Oxidation Processes (AOPs) in basic pH conditions
PublicationThe paper presents the results of studies on chemical treatment of effluents from production of bitumen of petroleum origin. Due to the presence of sulfide ions, the pH of these effluents is strongly alkaline. Several Advanced Oxidation Processes (AOPs) were studied, including the use of hydroxyl and sulfate radicals oxidants, the hydrodynamic cavitation as well as sonocavitation. The best processes allow to obtain 45% reduction...
-
Projekt belki hybrydowej 1b dla firmy H+H Polska sp. z o.o.
PublicationNiniejsze opracowanie stanowi projekt belki nadprożowej o długości 3,0 m o przekroju poprzecznym belki hybrydowej (beton konstrukcyjny łączony z betonem komórkowym) nawiązującej do wymiaru bloczka z betonu komórkowego firmy H+H Polska sp. z o.o. o wymiarach (szerokość × wysokość × długość): 10 × 24 × 60 cm, odmiany D600 o średniej wytrzymałości na ściskanie 3,0 MPa. Całkowita wysokość belki hybrydowej wynosi 24 cm.
-
Projekt belki hybrydowej 1a dla firmy H+H Polska sp. z o.o.
PublicationNiniejsze opracowanie stanowi projekt belki nadprożowej o długości 3,0 m o przekroju poprzecznym belki hybrydowej (beton konstrukcyjny łączony z betonem komórkowym) nawiązującej do wymiaru bloczka z betonu komórkowego firmy H+H Polska sp. z o.o. o wymiarach (szerokość × wysokość × długość): 24 × 24 × 60 cm, odmiany D600 o średniej wytrzymałości na ściskanie 3,0 MPa. Całkowita wysokość belki hybrydowej wynosi 27 cm (wraz z 3 cm...
-
Impact of inorganic ions and pH variations on toxicity and endocrine potential of selected environmentally relevant pharmaceuticals
PublicationAssessment of the impact of pharmaceutical residues on living organisms is a very complex subject. Apart from taking into account the toxicity of individual compounds, environmental factors should also be taken into account. In this paper, attempts were made to assess the impact of coexisting inorganic ions and changes in pH on the toxicity of ten selected pharmaceuticals. Two bioassays were used to measure the estrogenic and androgenic...
-
Release of selected chemical elements from shale drill cuttings to aqueous solutions of different pH
PublicationThe effect of pH changes on leachability of light and heavy metals from shale drill cuttings generated from unconventional shale gas production was investigated. Cuttings, being the primary byproduct generated from drilling operations, belong to the potentially hazardous type of wastes due to presence of heavy and radioactive elements and remains of drilling fluid. In this regard, assessment of potentially dangerous components...
-
Structural studies of a cold-adapted dimeric Beta-D-galactosidase from Paracoccus sp. 32d
PublicationThe crystal structure of a novel dimeric [beta]-D-galactosidase from Paracoccus sp. 32d (Par[beta]DG) was solved in space group P212121 at a resolution of 2.4 Å by molecular replacement with multiple models using the BALBES software. This enzyme belongs to glycoside hydrolase family 2 (GH2), similar to the tetrameric and hexameric [beta]-D-galactosidases from Escherichia coli and Arthrobacter sp. C2-2, respectively. It is the second...
-
Opracowanie ekspertyzy dotyczącej przyczyn pękania łopatek wirnika SP turbiny PG1 typu 13UP55
PublicationZakres badań i analiz dotyczył ustalenia przyczyn pękania elementów łopatek wirnika SPA5 turbiny TG1 typu 13UP55 obejmował: 1. wykonanie badań składu chemicznego stali, z której wykonano łopatki dostarczone przez Zleceniodawcę dwoma metodami, 2. badania mikroobszarów zniszczenia łopatek na mikroskopie elektronowym skaningowym z mikroanalizatorem EDS, 3. badania metalograficzne makroskopowe i mikroskopowe fragmentów...
-
Photosensitive and pH-dependent activity of pyrazine-functionalized carbazole derivative as promising antifungal and imaging agent
PublicationCarbazole skeleton plays a significant role as a structural scaffold of many pharmacologically active compounds. Pyrazine-functionalized carbazole derivative was constructed by coupling 2-amino-5-bromo-3-methylaminepyrazine (ABMAP) into 3 and 6 positions of the carbazole ring. Multi-experimental methods were used, e.g., potentiometric, spectroscopic (ATR, UV, XRD powder,1H and13C NMR), electrochemical (cyclic voltammetry), and...
-
pH-Induced modification to stratum corneum lipids investigated using thermal, spectroscopic, and chromatographic techniques
PublicationZbadano jakim zmianom jakościowym i ilościowym ulegają indywidualne klasy lipidów w Stratum Corneum (tkanka rogowa skóry) pod wpływem środowiska o zmiennym pH.
-
Effect of furfural on the enzyme activity during enzymatic hydrolysis of cellulose isolated from poplar wood (Populus sp.) Wpływ furfuralu na aktywność enzymu podczas hydrolizy celulozy wyodrębnionej z drewna topoli (Populus sp.)
Publication -
Influence of the boron doping level on the electrochemical oxidation of raw landfill leachates: advanced pre-treatment prior to the biological nitrogen removal
PublicationThe electrochemical oxidative treatment of landfill leachates (LLs) containing high amounts of ammonia nitrogen and organic matter was used as a promising method, prior to biological processes, to achieve the final effluent quality that would be acceptable by current regulations. The deposited boron-doped diamond electrodes (BDDs) with different boron doping concentrations (10000, 5000 and 500 ppm of B) were applied as anodes....
-
Retention time and peak width in the combined pH/organic modifier gradient high performance liquid chromatography
Publication -
Antarctic, cold-adapted beta-galactosidase of Pseudoalteromonas sp. 22b as an effective tool for alkyl galactopyranosides synthesis
PublicationPrzedstawione jest zastosowanie arktycznej beta-galaktozydazy Pseudoalteromonas sp. 22b do katalizy syntezy alkilowych galaktopyranozydów. Njwyższą wydajność syntezy uzyskuje się przy pH 6-9 i 10-30% stężenia wody. Podobnie jak dla mezofilnych beta-galaktozydaz enzym antarktyczny syntetyzował najwydajniej kiedy reakcja przebiega w mieszaninie buforu i rozpuszczalnika organiczego (poniżej 50%, v/v).
-
Soil pH effects on the comparative toxicity of dissolved zinc, non-nano and nano ZnO to the earthwormEisenia fetida
Publication -
Molecular dynamics simulations of the affinity of chitin and chitosan for collagen: the effect of pH and the presence of sodium and calcium cations
PublicationChitosan and chitin are promising biopolymers used in many areas including biomedical applications, such as tissue engineering and viscosupplementation. Chitosan shares similar properties with hyaluronan, a natural component of synovial fluid, making it a good candidate for joint disease treatment. The structural and energetic consequences of intermolecular interactions are crucial for understanding the biolubrication phenomenon...
-
SP-1, a Serine Protease from the Gut Microbiota, Influences Colitis and Drives Intestinal Dysbiosis in Mice
Publication -
Study of Different Advanced Oxidation Processes for Wastewater Treatment from Petroleum Bitumen Production at Basic pH
PublicationEffluents from production of petroleum bitumens were submitted to treatment by three different AOPs at basic pH (i.e., O3, H2O2 and the combination of O3 and H2O2, a so-called peroxone). The paper presents studies on the identification and monitoring of the volatile organic compounds (VOCs) degradation present in the effluents and formation of byproducts, COD, BOD5, sulfide ions, biotoxicity, and biodegradability changes during...
-
BIOSYNTEZA ESTERAZY Z PSEUDOMONAS SP. S9 W KOMÓRKACH PICHIA PASTORIS, OCZYSZCZANIE I CHARAKTERYSTYKA
PublicationEnzymy lipolityczne ze względu na swoje unikalne właściwości takie jak stereo-, chemo- i regiospecyficzność znajdują szereg zastosowań w różnych gałęziach przemysłu. Lipazy i esterazy wykorzystywane są w przemyśle chemicznym, farmaceutycznym, spożywczym oraz w syntezie organicznej. W ramach wcześniejszych badań zidentyfikowałam i scharakteryzowałam aktywną w niskiej temperaturze esterazę z Pseudomonas sp. S9. Esteraza Pseudomonas...
-
Inhibition of amyloid fibril formation of hen egg white lysozyme by trimethylamine N-oxide at low pH
PublicationIn vitro inhibition of the formation of fibrous aggregates of proteins (amyloids) has gained increasing attention due to the number of diseases associated with protein misfolding and fibrillation. An interesting group of compounds for which pronounced activity against this phenomenon can be expected consists of low molecular weight substances (osmolytes) which have the ability to change protein stability. Here we investigate the...
-
Importance of the combined effects of dissolved oxygen and pH on optimization of nitrogen removal in anammox-enriched granular sludge
PublicationThe combined effects of dissolved oxygen (DO) and pH on nitrogen removal were investigated in a laboratory-scale sequencing batch reactor (SBR) with anammox-enriched granular sludge obtained from a nitritation/anammox system. The highest specific nitrogen removal rate (SNRR) (1.1 gN gVSS−1 d−1) was observed under non-aerated conditions, resulting in the nitrogen removal efficiency of 81.6%. Although nitrogen removal was readily...
-
Comparison of the content of phenolic compounds and antioxidant activity of white, red and black currants (Ribes sp.) extracts
PublicationThe aim of this study was to compare the content of antioxidant compounds and the antioxidant activity of extracts from white, red and black currants (Ribes sp.). The chemical properties verified included determinations of anthocyanins and other phenols by HPLC-DAD-MS, total antioxidant activity by standard spectrophotometric tests (ABTS, DPPH and FCR), and profiles of antioxidants by TLC with visualization reagents (ABTS, DPPH,...
-
Mapping the Structural and Dynamic Determinants of pH-Sensitive Heparin Binding to Granulocyte Macrophage Colony Stimulating Factor
PublicationGranulocyte macrophage colony stimulating factor (GMCSF) is an immunomodulatory cytokine that is harnessed as a therapeutic. GMCSF is known to interact with other clinically important molecules, such as heparin, suggesting that endogenous and administered GMCSF has the potential to modulate orthogonal treatment outcomes. Thus, molecular level characterization of GMCSF and its interactions with biologically active compounds is critical...
-
Mapping the Transglycosylation Relevant Sites of Cold-Adapted β-D-Galactosidase from Arthrobacter sp. 32cB
PublicationB-Galactosidase from Arthrobacter sp. 32cB (ArthbetaDG) is a cold-adapted enzyme able to catalyze hydrolysis of beta-D-galactosides and transglycosylation reaction, where galactosyl moiety is being transferred onto an acceptor larger than a water molecule. Mutants of ArthbetaDG D207A and E517Q were designed to determine the significance of specific residues and to enable formation of complexes with lactulose and sucrose and to...
-
Helicobacter muridarum sp. nov., a Microaerophilic Helical Bacterium with a Novel Ultrastructure Isolated from the Intestinal Mucosa of Rodents
Publication -
Purification and biochemical characteristic of a cold-active recombinant esterase from Pseudoalteromonas sp. 643A under denaturing conditions.
PublicationIn this paper production of a cold-active esterase EstA from the Antarctic bacterium Pseudoalteromonas sp. 643A in E. coli expression system was described. The purification and biochemical characteristic of EstA were performed in the presence of urea and then compared with results obtained for the esterase with no addition of urea and isolated from the native source. In both cases the cold-active enzyme displayed similar properties....
-
Effects of different omeprazole dosing on gastric pH in non-variceal upper gastrointestinal bleeding: A randomized prospective study
Publication -
A new cold-active β-galactosidase from Arthrobacter sp. S3* - gene cloning, overexpression, purification and properties
PublicationA psychrotrophic bacterium producing a cold-active β-galactosidase was isolated from Spitsbergen soil and classified as Arthrobacter sp. S3*. The gene encoding β-galactosidase was isolated from the genomic DNA library, sequenced, cloned, expressed in Escherichia coli, purified by ion exchange chromatography and characterized. The Arthrobaster sp. S3* β-galactosidase is a homotrimeric enzyme composed of 74,4 kDa subunits. It is...
-
Cloning, expression in Komagataella phaffii, and biochemical characterization of recombinant sequence variants of Pseudomonas sp. S9 GDSL-esterase
PublicationTwo recombinant Komagataella phaffii (formerly Pichia pastoris) yeast strains for production of two sequential variants of EstS9 esterase from psychrotolerant bacterium Pseudomonas sp. S9, i.e. αEstS9N (a two-domain enzyme consisting of a catalytic domain and an autotransporter domain) and αEstS9Δ (a single-domain esterase) were constructed. However, only one of recombinant K. phaffii strains, namely Komagataella phaffii X-33/pPICZαestS9Δ,...
-
Projekt belki hybrydowej 1a dla firmy H+H Polska sp. z o.o. wariant 2
PublicationNiniejsze opracowanie stanowi projekt belki nadprożowej o długości 3,0 m o przekroju poprzecznym belki hybrydowej (beton konstrukcyjny łączony z betonem komórkowym) nawiązującej do wymiaru bloczka z betonu komórkowego firmy H+H Polska sp. z o.o. o wymiarach (szerokość × wysokość × długość): 24 × 24 × 60 cm, odmiany D600 o średniej wytrzymałości na ściskanie 3,0 MPa. Całkowita wysokość belki hybrydowej wynosi 27 cm (wraz z 3 cm...
-
Projekt belki hybrydowej 1b dla firmy H+H Polska sp. z o.o. wariant 2
PublicationNiniejsze opracowanie stanowi projekt belki nadprożowej o długości 3,0 m o przekroju poprzecznym belki hybrydowej (beton konstrukcyjny łączony z betonem komórkowym) nawiązującej do wymiaru bloczka z betonu komórkowego firmy H+H Polska sp. z o.o. o wymiarach (szerokość × wysokość × długość): 10 × 24 × 60 cm, odmiany D600 o średniej wytrzymałości na ściskanie 3,0 MPa. Całkowita wysokość belki hybrydowej wynosi 24 cm.
-
A novel cold-active beta-D-galactosidase from the Paracoccus sp. 32d - gene cloning, purification and characterization
PublicationBeta-D-galactosidase (EC 3.2.1.23) catalyze the hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Cold-active beta-D-galactosidases have recently become a focus of attention of researchers and dairy product manufactures owing to theirs ability to: (I) eliminate of lactose from refrigerated milk for people afflicted with lactose intolerance, (II) convert lactose to glucose and galactose which...
-
Highly effective degradation of selected groups of organic compounds by cavitation based AOPs under basic pH conditions
PublicationCavitation has become on the most often applied methods in a number of industrial technologies. In the case of oxidation of organic pollutants occurring in the aqueous medium, cavitation forms the basis of numerous advanced oxidation processes (AOPs). This paper presents the results of investigations on the efficiency of oxidation of the following groups of organic compounds: organosulfur, nitro derivatives of benzene, BTEX, and...
-
Cloning, expression, and purification of a recombinant cold-adapted β-galactosidase from antarctic bacterium Pseudoalteromonas sp. 22b
PublicationAntarktyczny szczep, gram-ujemnych bakterii Pseudoalteromonas sp. 22b, wyizolowanych z przewodu pokarmowego kryla z gatunku Thyssanoessa macrura, jest producentem wewnątrzkomórkowej zimnolubnej beta-galaktozydazy. Białko to jest homotetramerem, w którym każda z jednostek monomeru zbudowana jest z 1028 reszt aminokwasowych. Gen kodujący ten enzym został sklonowany i eksprymowany w komórkach E. coli. Następnie z zastosowaniem procedury...
-
Measurements of absorption and emission spectra of tryptophan in solutions of different pH
Open Research DataInvestigations of the absorption and fluorescence spectra of tryptophan in different environments were carried out, controlling the pH of the environment and the concentration of tryptophan.
-
Genome Mining Revealed a High Biosynthetic Potential for Antifungal Streptomyces sp. S-2 Isolated from Black Soot
Publication -
Picomolar analysis of flavins in biological samples by dynamic pH junction-sweeping capillary electrophoresis with laser-induced fluorescence detection
Publication -
Psychometric Properties of the Polish Version of the FACIT-Sp-12: Assessing Spiritual Well-Being Among Patients with Chronic Diseases
Publication -
Solid-Contact pH Sensor without CO2Interference with a Superhydrophobic PEDOT-C14as Solid Contact: The Ultimate “Water Layer” Test
Publication -
Analiza przyczyn zwarcia w rozdzielnicy 15 kV w Masfalt sp. z o. o. w Elblągu
PublicationOpisano przyczynę i skutki zwarcia łukowego o czasie trwania nie przekraczającym 10 sekund w rozdzielnicy średniego napięcia 3 polowej typu RSL o napięciu roboczym 24 kV.
-
Influence of pH of sol-gel solution on phase composition and photocatalytic activity of TiO 2 under UV and visible light
Publication -
Active Site Architecture and Reaction Mechanism Determination of Cold Adapted beta-D-galactosidase from Arthrobacter sp. 32cB
PublicationArthbetaDG is a dimeric, cold-adapted beta-D-galactosidase that exhibits high hydrolytic and transglycosylation activity. A series of crystal structures of its wild form, as well as its ArthbetaDG_E441Q mutein complexes with ligands were obtained in order to describe the mode of its action. The ArthbetaDG_E441Q mutein is an inactive form of the enzyme designed to enable observation of enzyme interaction with its substrate. The...
-
Poly-L-Lysine-functionalized fluorescent diamond particles: pH triggered fluorescence enhancement via surface charge modulation
PublicationRecently, the interest in applying fluorescent diamond particles (FDPs) containing nitrogen-vacancy (NV) centers for enhancing the mechanical and chemical properties of some materials, biological imaging, and sensing has been expanding rapidly. The unique properties of NV centers such as intensive, time-stable fluorescence, and an electron spin, which exhibits long coherence time and may be manipulated using external stimuli, such...
-
Laboratoryjny bioreaktor z systemem kontroli pH do badań nad wytwarzaniem wodoru w procesie ciemnej fermentacji
PublicationW pracy przedstawiono konstrukcję niskokosztowego bioreaktora badawczego wyposażonego w sys-tem kontroli i regulacji pH. Zaprezentowano jego wykorzystanie do badania produkcji wodoru w pro-cesie fermentacji ciemnej z zastosowaniem Enterobacter aerogenes ATCC 13048. W pracy porównano wyniki uzyskane przy prowadzeniu procesu bez i z regulacją pH dla różnych materiałów wsadowych: glukozy, hydrolizatów topoli energetycznej, serwatki...
-
Application of Wavelet Transform and Fractal Analysis for Esophageal pH-Metry to Determine a New Method to Diagnose Gastroesophageal Reflux Disease
PublicationIn this paper, a new method for analysing gastroesophageal reflux disease (GERD) is shown. This novel method uses wavelet transform (WT) and wavelet-based fractal analysis (WBFA) on esophageal pH-metry measurements. The esophageal pH-metry is an important diagnostic tool supporting the physician’s work in diagnosing some forms of reflux diseases. Interpreting the results of 24-h pH-metry monitoring is time-consuming, and the conclusions...
-
B-GALACTOSIDASE ARTHROBACTER SP. 32cB - OBTAINING THE GENE SEQUENCE, CONSTRUCTION OF THE EXPRESSION SYSTEM, BIOSYNTHESIS AND BIOCHEMICAL CHARACTERIZATION OF THE ENZYME
PublicationINTRODUCTION: β-Galactosidase is an enzyme which catalyzes the hydrolysis of O glycosidic bond in β-galactosides. Another activity of β galactosidase is a transglycosylation activity. The main industrial use of this protein is the hydrolysis of lactose in milk in a cooling conditions. Synthesis of galactooligosaccharides, which are mostly used as a prebiotics added to some foods or available as dietary supplements, is only one...
-
Dokumentacja oraz wnioski z badań belek hybrydowych dla firmy H+H Polska sp. z o.o.
PublicationOpracowanie stanowi dokumentację oraz wnioski z badań belek nadprożowych o długości 3,0 m każda o przekroju poprzecznym belki hybrydowej (beton konstrukcyjny łączony z betonem komórkowym) nawiązującej do wymiaru bloczka z betonu komórkowego firmy H+H Polska sp. z o.o. o wymiarach (szerokość × wysokość × długość): 24 × 24 × 60 cm, odmiany D600 o średniej wytrzymałości na ściskanie 3,0 MPa (zgodnie z kartą techniczną w załączniku...
-
Cloning, expression, and biochemical characterization of a coldactive GDSL-esterase of a Pseudomonas sp. S9 isolated from Spitsbergen island soil
PublicationAn estS9 gene, encoding an esterase of the psychrotolerant bacterium Pseudomonas sp. S9 was cloned and sequenced. The deduced sequence revealed a protein of 636 amino acid residues with a molecular mass of 69 kDa.Further amino acid sequence analysis revealed that the EstS9 enzyme contained a G-D-S-L motif centered at a catalytic serine, an N-terminal catalytic domain and a C-terminal autotransporter domain. Two recombinant E. coli...