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Wyniki wyszukiwania dla: GENE EXPRESSION REGULATION
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A study on the interaction of rhodamine B with methylthioadenosine phosphorylase protein sourced from an Antarctic soil metagenomic library.
PublikacjaThe presented study examines the phenomenon of the fluorescence under UV light excitation (312 nm) of E. coli cells expressing a novel metagenomic-derived putative methylthioadenosine phosphorylase gene, called rsfp, grown on LB agar supplemented with a fluorescent dye rhodamine B. For this purpose, an rsfp gene was cloned and expressed in an LMG194 E. coli strain using an arabinose promoter. The resulting RSFP protein was purified...
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Laboratory bioreactor with pH control system for investigations of hydrogen production in the dark fermentation process
PublikacjaThe work presents the construction of a low-cost research bioreactor equipped with a pH control sys-tem. The developed bioreactor was applied for hydrogen production in the dark fermentation process using Enterobacter aerogenes ATCC 13048. Presented paper compares the results obtained during the process without and with pH regulation for different feed materials: glucose, energetic poplar hydro-lysates, acid whey and glycerol....
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Impact of information systems (IS) infusion on Open Government Data (OGD) adoption
PublikacjaPurpose – This study aims to underline the possible influence of the moderator, information systems (IS) infusion, on Open Government Data (OGD) adoption and usage. Design/methodology/approach – Using the partial least squares-structural equation modeling methodological approach, the adapted unified theory of acceptance and use of technology (UTAUT) model has been used for understanding the role of themoderating variable, namely,...
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Whither the need and motivation for open government data (OGD) promotional strategies?
PublikacjaPurpose It has been underscored in the extant literature that open government data (OGD) has not percolated across the length and breadth of any country, let alone the awareness of the OGD among the stakeholders themselves. In this vein, this study aims to underline the reasons as to why OGD promotion merits consideration apart from underlining the manner in which OGD promotion may be done. Design/methodology/approach Based on...
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Construction and purification of his6-Thermus thermophilus MutS protein
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Construction, purification, and functional characterization of His-tagged Candida albicans glucosamine-6-phosphate synthase expressed in Escherichia coli
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Purification to Homogeneity of Candida albicans Glucosamine-6-phosphate Synthase Overexpressed in Escherichia coli
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Extracellular secretion of Pseudoalteromonas sp. cold-adapted esterase in Escherichia coli in the presence of Pseudoalteromonas sp. components of ABC transport system
PublikacjaEnzymy lipolityczne, głównie lipazy i esterazy, stanowią cenną grupę biokatalizatorów wykorzystywanych w przemyśle. Coraz większe zainteresowanie tą klasą enzymów sprawiło, że poszukuje się alternatywnych źródeł ich pozyskiwania. W celu zbadania wpływu białek transportu ABC Pseudoalteromonas sp. 643A na ekspresję genu, sekrecję i aktywność esterazy EstA z tego mikroorganizmu w systemie ekspresyjnym E. coli skonstruowano układ do...
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Construction and purification of his6-Thermus thermophilus MutS protein.
PublikacjaGen mutS z termofilnej bakterii Thermus thermophilus zamplifikowano przy użyciu PCR, sklonowano i przeprowadzono jego ekspresję w E. coli. Rekombinantowe białko MutS zawierające oligohistydynową domenę na N-końcu oczyszczono w jednoetapowej procedurze przy wykorzystaniu chromatografii powinowactwaNi(2+). Zdolność do rozpoznania niekomplementarności białka his(6)-MutS potwierdzono w eksperymentach ochrony DNA przed trawieniem...
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Molecular and structural basis of inner core lipopolysaccharide alterations in Escherichia coli: incorporation of glucuronic acid and phosphoethanolamine in the heptose region.
PublikacjaIt is well established that lipopolysaccharide (LPS) often carries nonstoichiometric substitutions in lipid A and in the inner core. In this work, the molecular basis of inner core alterations and their physiological significance are addressed. A new inner core modification of LPS is described, which arises due to the addition of glucuronic acid on the third heptose with a concomitant loss of phosphate on the second heptose. This...
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Virulence analysis and antibiotic resistance of Klebsiella pneumoniae isolates from hospitalised patients in Poland
PublikacjaKlebsiella pneumoniae (KP) is a nosocomial pathogen causing difficult-to-treat infections. The presence of virulence genes and antibiotic resistance of 109 KP isolates from hospitalized patients were investigated. Among them, 68.8% were multi-drug resistant (MDR) and 59.6% produced extended-spectrum beta-lactamases (ESBLs). Metallo-β-lactamases (MBLs) were produced by 22% of isolates (mainly from anus), including 16.5% of isolates...
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Strahlungslose Übertragung von Elektronenanregungsenergie bei zweidimensionalen lumineszierenden Systemen
PublikacjaAn expression for the photoluminescence (PhL) quantum yield of donors in two-dimensional systems as depending on the concentration ratio nD'/nA' of donors and acceptors has been obtained. In the particular case nD' ⪡ nA' the expression reduces to the form given by other authors. The obtained formula can also be applied to the description of the concentrational quenching of PhL when dimers act as acceptors. The theory has been...
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Exterior Plasterwork in Gdynia`s Modernist Architecture and Its Preservation
PublikacjaText presents the historical plasterworks of facades of modernist monuments built before WWII as the important expression in architecture and ist conservation
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Production of trehalose in a single step enzymatic reaction
PublikacjaThe aim of this study was isolation and cloning of trehalose synthase gene derived from extremophilic microorganisms Deinoccoci to the expressive E. coli vectors and its biosythesis in different hosts.
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Compulsive sexual behavior and dysregulation of emotion
PublikacjaIntroduction Dysregulation of emotion (DE) is commonly seen in individuals suffering from compulsive sexual behavior (CSB), as well as represents a crucial element of its common comorbidities like mood, anxiety, and substance use disorders. Aim To investigate the links between CSB and DE. Methods A review of pertinent literature on CSB and DE was performed using EBSCO, PubMed, and Google Scholar databases. Main Outcome Measure...
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Secondary emission influenced fluorescence decay of a homogeneous fluorophore solution
PublikacjaAn analytical expression is found allowing the calculation of the secondary emission influenced fluorescence decay of a homogeneous fluorophore solution. Before starting the calculation one has to know the shape of the primary fluorescence decay of the fluorophore and the value of the parameter kappa denoting the ratio of the secondary to primary steady-state fluorescence intensities. The method elaborated by Budo and Ketskemety...
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Lucyna Holec-Gąsior dr hab. inż.
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Neurochemical changes underlying cognitive impairment in olfactory bulbectomized rats and the impact of the mGlu5-positive allosteric modulator CDPPB
PublikacjaThe olfactory bulbectomized (OBX) rat model is a well-established model of depression in which antidepressant drugs reverse deficits in the passive avoidance test 14 days after administration. Recently, the olfactory bulbectomized rat model has been proposed to be a model of Alzheimer’s disease (AD), and the available data indicate similarities between the changes that typically occur in AD and those observed in OBX animals. In...
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Research of Emergency Modes of Wind Power Plants Using Computer Simulation
PublikacjaThe aim of this study is to investigate changes in the wind power plant energy production parameters under the conditions of sudden wind changes and voltage drop. To achieve these goals, a simulation of operation of wind power plants was performed. Twelve wind turbines with variable rotational speed equipped with a Fuhrländer FL 2500/104 asynchronous double-fed induction generator (DFIG) were used, each with an installed capacity...
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Immunological response of sheep to injections of plasmids encoding Toxoplasma gondii SAG1 and ROP1 genes
PublikacjaInfection with the intracellular protozoan parasite Toxo¬plasma gondii (T. gondii) causes health problems to both humans and livestock and has a large economic impact worldwide. The immune response in sheep following infection with T. gondii was evaluated using six different combinations of plasmid DNA, recombinant antigen and adjuvant. Sheep were generally vaccinated twice by intramuscular injection with plasmid DNA containing...
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Biofilm Production and Presence of ica and bap Genes in Staphylococcus aureus Strains Isolated from Cows with Mastitis in the Eastern Poland
PublikacjaThe aim of the study was phenotypic and genotypic analysis of 132 S. aureus strains isolated from mastitis in eastern Poland in respect to their biofilm formation ability. The analysis of the size polymorphism of fragment X in the gene encoding protein A (spa) revealed high genetic differentiation of the analyzed group of isolates. The ability of biofilm formation by the isolates was tested using two phenotypic methods. The Congo...
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RAGE as a Novel Biomarker for Prostate Cancer: A Systematic Review and Meta-Analysis
PublikacjaThe receptor for advanced glycation end-products (RAGE) has been implicated in driving prostate cancer (PCa) growth, aggression, and metastasis through the fueling of chronic inflammation in the tumor microenvironment. This systematic review and meta-analysis summarizes and analyzes the current clinical and preclinical data to provide insight into the relationships among RAGE levels and PCa, cancer grade, and molecular effects....
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Emotional distress, burnout and sense of safety during the COVID-19 pandemic in teachers after the reopening of schools
PublikacjaThe COVID-19 pandemic is having a significant impact on people's psychological well-being and mental health. This study aimed to identify factors linked to emotional distress, burnout and sense of safety in teachers related to the reopening of Polish schools after lockdown, remote work, and the holiday period between March and August 2020. A total of 1,286 teachers from different educational institutions participated in the...
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HOW EFFICIENT IS NOISE LABELING OF TIRES?
PublikacjaSince 2012 all new tires in Europe must be labeled. The label contains general information about tire performance concerning rolling resistance (that corresponds to fuel economy), noise emission and wet grip (only for passenger car tires). Measurements of noise performance of tires must be performed according to the Annex 3 of the Regulation No 117 of the Economic Commission for Europe of the United Nations. The regulation specifies...
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Fast implementation of FDTD-compatible green's function on multicore processor
PublikacjaIn this letter, numerically efficient implementation of the finite-difference time domain (FDTD)-compatible Green's function on a multicore processor is presented. Recently, closed-form expression of this discrete Green's function (DGF) was derived, which simplifies its application in the FDTD simulations of radiation and scattering problems. Unfortunately, the new DGF expression involves binomial coefficients, whose computations...
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Novel application of terminal restriction fragments length polymorphism method (t-RFLP) for identification of six clinically important Candida sp.
PublikacjaTerminal restriction fragments length polymorphism method (t-RFLP) is a modified classical RFLP method. The main difference is based on carrying out PCR using a fluorescently labeled primer or primers. The result of such modification is that after digestion of amplicons only terminal fragments of PCR product (containing fluorescent dye) are visualized. T-RFLP method has found application in environmental microbiology and epidemiology....
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Should Polish banks be domesticated?
PublikacjaThe financial crisis revealed vulnerabilities in the regulation and supervision of the banking system. The overarching goal of the proposal (Bazylea III and CRD IV) is to strengthen the resilience of the EU banking sector. The effects of these regulations will be detrimental to the Polish banking system and economy. This follows from the fact that listed banks in Poland are subsidiaries of international banking groups. The article...
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Novel single-stage buck-boost inverter with unfolding circuit
PublikacjaA novel solution of a single-stage buck-boost inverter with unfolding circuit at the output stage is presented. The inverter has a wide range of input voltage regulation, minimum passive components and a very flexible control structure. It can be applied for renewable energy systems where high power density is required. Our simulation results confirmed all theoretical statements
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Metaheurystyki w kolorowaniu grafów
PublikacjaW rozdziale opisano cztery metaheurystyki wykorzystywane w problemie koloro-wania grafów: symulowane wyżarzanie, przeszukiwanie tabu, algorytmy gene-tyczne, algorytmy mrówkowe. Skupiono się głównie na zagadnieniach wykorzys-tania tych metod w badanym problemie.
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Properties of recombinant trehalose synthase from Deinococcus radiodurans expressed in Escherichia coli
PublikacjaA trehalose synthase gene from Deinococcus radiodurans(DSMZ 20539)containing 1659 bp reading frame encoding 552 amino acids was amplified using PCR. The gene was finally ligated into pET30Ek/LIC vector and expressed after isopropyl β-d-thiogalactopyranoside induction in Escherichia coli (DE3) Rosetta pLysS. The recombinanttrehalose synthase (DraTreS) containing a His6-tag at the C-terminus was purified by metal affinity chromatography...
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Modulation of CYP3A4 activity and induction of apoptosis, necrosis and senescence by the antitumor imidazoacridinone C-1311 in human hepatoma cells
PublikacjaThere is increasing evidence that the expression level of drug metabolic enzymes affects the final cellular response following drug treatment. Moreover, anti-tumour agents may modulate enzymatic activity and/or cellular expression of metabolic enzymes in tumour cells. We investigated the influence of CYP3A4 overexpression on the cellular response induced by the anti-tumour agent C-1311 in hepatoma cells. C-1311-mediated CYP3A4...
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Obrony prac doktorskich na Wydziale Chemicznym
WydarzeniaDn. 25.02.2022 r. o godz. 10.15 w Audytorium 1.4 Wydziału Chemicznego PG (budynek nr 5) odbędzie się w trybie hybrydowym publiczna obrona pracy doktorskiej mgr inż. Darii Biernackiej.
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On Effective Bending Stiffness of a Laminate Nanoplate Considering Steigmann–Ogden Surface Elasticity
PublikacjaAs at the nanoscale the surface-to-volume ratio may be comparable with any characteristic length, while the material properties may essentially depend on surface/interface energy properties. In order to get effective material properties at the nanoscale, one can use various generalized models of continuum. In particular, within the framework of continuum mechanics, the surface elasticity is applied to the modelling of surface-related...
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Magnetizability of the relativistic hydrogenlike atom in an arbitrary discrete energy eigenstate: Application of the Sturmian expansion of the generalized Dirac-Coulomb Green function
PublikacjaThe Sturmian expansion of the generalized Dirac--Coulomb Green function [R.\/~Szmytkowski, J.\ Phys.\ B 30 (1997) 825; erratum 30 (1997) 2747] is exploited to derive a closed-form expression for the magnetizability of an arbitrary discrete state of the relativistic one-electron atom with a point-like, spinless and motionless nucleus of charge $Ze$. The result has the form of a double finite sum involving the generalized hypergeometric...
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Facial emotion recognition using depth data
PublikacjaIn this paper an original approach is presented for facial expression and emotion recognition based only on depth channel from Microsoft Kinect sensor. The emotional user model contains nine emotions including the neutral one. The proposed recognition algorithm uses local movements detection within the face area in order to recognize actual facial expression. This approach has been validated on Facial Expressions and Emotions Database...
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Docking simulations, Molecular properties and ADMET studies of novel Chromane6,7diol analogues as potential inhibitors of Mushroom tyrosinase
PublikacjaResearch on inhibition of tyrosinase enzyme has attained significant value, because tyrosinase inhibitors have potential applications in medicine, cosmetics (as whitening agents) and in agriculture (as bioinsecticides). Determination and elucidation of new tyrosinase inhibitors are not only beneficial for medical purposes, but their promising applications in improving food quality and nutritional...
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Discrimination of hospital isolates of Acinetobacter baumannii using repeated sequences and whole genome alignment differential analysis
PublikacjaAn optimized method for bacterial strain differentiation, based on combination of Repeated Sequences and Whole Genome Alignment Differential Analysis (RS&WGADA), is presented in this report. In this analysis, 51 Acinetobacter baumannii multidrug-resistance strains from one hospital environment and patients from 14 hospital wards were classified on the basis of polymorphisms of repeated sequences located in CRISPR region, variation...
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CaZnO-based nanoghosts for the detection of ssDNA, pCRISPR and recombinant SARS-CoV-2 spike antigen and targeted delivery of doxorubicin
PublikacjaOverexpression of proteins/antigens and other gene-related sequences in the bodies could lead to significant mutations and refractory diseases. Detection and identification of assorted trace concentrations of such proteins/antigens and/or gene-related sequences remain challenging, affecting different pathogens and making viruses stronger. Correspondingly, coronavirus (SARS-CoV-2) mutations/alterations and spread could lead to overexpression...
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Benchmark of the traffic congestion in electrical transport by means of multi criteria decision analysis
PublikacjaCongestion of the road traffic is an important aspect related to the issues of energy consumption in public transport. Due to the multiattribute nature, the expression of traffic congestion in a quantitative valueis difficult to achieve. The article presents a method of estimation of traffic congestion by means of a multiattribute decision analysis.
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Red Kale (Brassica oleracea L. ssp. acephala L. var. sabellica) Induces Apoptosis in Human Colorectal Cancer Cells In Vitro
PublikacjaThis article presents the results of studies investigating the effect of red kale (Brassica oleracea L. ssp. acephala L. var. sabellica) extract on cancer cells (HT-29). The cytotoxicity of the red kale extract was assessed using MTT and LDH assays, while qRT-PCR was employed to analyze the expression of genes associated with the p53 signaling pathway to elucidate the effect of the extract on cancer cells. Furthermore, HPLC-ESI-QTOF-MS/MS...
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Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells
PublikacjaAdipose-derived stem cells (ASCs) have become an important research model in regenerative medicine. However, there are controversies regarding the impact of prolonged cell culture on the ASCs phenotype and their differentiation potential. Hence, we studied 10 clinical ASCs replicates from plastic and oncological surgery patients, in six-passage FBS supplemented cultures. We quantified basic mesenchymal cell surface marker transcripts...
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Molecular differences in mitochondrial DNA (mtDNA) genomes of dogs with malignant mammary tumours
Dane BadawczeThe aim of this study was to determine molecular defects in mitochondrial DNA with the use of large-scale genome analysis in malignant canine mammary gland tumours and indicate whether these changes were linked with the carcinogenesis process. With the use of the NGS technology, we sequenced 27 samples of mtDNA isolated from blood and tumours obtained...
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Naturally occurring mitochondrial DNA heteroplasmy in the MRL mouse
PublikacjaThe MRL/MpJ mouse is an inbred laboratory strain of Mus musculus, known to exhibit enhanced autoimmunity, increased wound healing, and increased regeneration properties. We report the full-length mitochondrial DNA (mtDNA) sequence of the MRL mouse (Accession # EU450583), and characterize the discovery of two naturally occurring heteroplasmic sites. The first is a T3900C substitution in the TpsiCloop of the tRNA methionine gene...
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Robot Eye Perspective in Perceiving Facial Expressions in Interaction with Children with Autism
PublikacjaThe paper concerns automatic facial expression analysis applied in a study of natural “in the wild” interaction between children with autism and a social robot. The paper reports a study that analyzed the recordings captured via a camera located in the eye of a robot. Children with autism exhibit a diverse level of deficits, including ones in social interaction and emotional expression. The aim of the study was to explore the possibility...
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matK sequences alignment of Oncidium Sw. species
Dane BadawczeComparison of matK gene sequences of 28 species of Oncidium Sw.. Alignment was created using Clustal Omega ver.1.2.4 in SnapGene software 7.1.1.
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Liquid residence time in vortex separators
PublikacjaCentrifugal force enhances gravitational separation of suspension (vortex separators), as well as allows regulation of liquid discharge (vortex flow controls). Furthermore, it elongates the effective residence time of liquid inside the chamber in comparison to non-circulative flow. Laboratorz measurements, described in the paper, indicate that average liquid residence time for circulative flow exceeds 50% of plug-flow time,...
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Bioelectronic nose: Current status and perspectives
PublikacjaA characteristic feature of human and animal organs of smell is the ability to identify hundreds of thousands of odours. It is accompanied by particular smell sensations, which are a basic source of information about odour mixture. The main structural elements of biological smell systems are the olfactory receptors. Small differences in a structure of odorous molecules (odorants) can lead to significant change of odour, which is...
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Tetracycline and ampicillin resistance of Escherichia coli strains of surface water origin: the potential for horizontal transfer of resistance genes = Oporność na tetracyklinę i ampicylinę szczepów Escherichia Coli wyizolowanych z wody powierzchniowej : możliwość horyzontalnego przenoszenia genów oporności
PublikacjaThe aim of this preliminary study was to assess the occurrence and molecular diversity of tetracycline and ampicillin resistance genes carried by Escherichia coli present in surface water. Bacterial strains were isolated from two watercourses (the Reda River and the Oliwski Stream) that influence the quality of coastal waters in the Gdańsk Bay (Northern Poland) by direct discharge. The bacterial drug susceptibility, tested against...
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The Caucasian flora: a still-to-be-discovered rich source of antioxidants
PublikacjaCellular redox homeostasis is a state of balance between the formation of Usually Reactive Oxygen and / or Nitrogen Species (ROS/RNS), endogenous antioxidant defence systems, and exogenous dietary antioxidants. The disturbance of redox homeostasis, by the overproduction of endogenous ROS/RNS, may increase the risk of development of so-called civilisation diseases. The solution seems to be either the increased production of endogenous...
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The generalization of objects representing groups of buildings in the Kartuzy district by simplification operator with the Simplify Building tool - scale 1:10000
Dane BadawczeThe process of automatic generalization is one of the elements of spatial data preparation for the purpose of creating digital cartographic studies. The presented data include a part of the process of generalization of building groups obtained from the national geodesy and cartography resource from BDOT10k (10k topographic database) [1].