Wyniki wyszukiwania dla: liquid chromatography
-
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Czasopisma -
Liquid chromatography techniques for separation of flavonoids from Droseraceae
PublikacjaFlavonoids which are presented in insectivorous plants of the Droseraceae family have wide range of advantageous properties i.e. an antioxidant, anti-inflammatory and antimicrobial activities, antitumor activity was reported as well.The purpose of the research was to develop the most favourable conditions for liquid chromatographic separation and identification of myricetin and quercetin in extracts of insectivorous plants. In...
-
Calixarenes as stationary phases in High Performance Liquid Chromatography
PublikacjaPraca przeglądowa przedstawia dotychczasowe metody syntezy faz stacjonarnych na bazie pochodnych kaliksarenów, kaliksrezorcyarenów i kalikspiroli chemicznie ziązanych z żelem krzemionkowym lub dynamicznie na nim obsadzonych. Podsumowano zastosowanie tych faz w rozdzielaniu szeregu klas związków organicznych i nieorganicznych metodą wysokosprawnej chromatografii cieczowej.
-
Free silanols and ionic liquids as their suppressors in liquid chromatography
Publikacja -
pH gradient high-performance liquid chromatography: theory and applications
Publikacja -
In-Syringe Dispersive Liquid-Liquid Microextraction Coupled with High- Performance Liquid Chromatography for Trace Analysis of Naphthalene Sulfonates in Seawater
Publikacja -
The simultaneous determination of hydrophobicity and dissociation constant by liquid chromatography–mass spectrometry
Publikacja -
Quantitative structure–retention relationships in affinity high-performance liquid chromatography
Publikacja -
Application of normal-phase high-performance liquid chromatography followed by gas chromatography for analytics of diesel fuel additives
PublikacjaThe paper presents the results of investigations on new procedures of determination of selected cleaning additives in diesel fuel. Two procedures: one-step analysis using gas chromatography with flame ionization detection (GC-FID) or mass spectrometry (GC-MS) and a two-step procedure in which normal-phase high-performance liquid chromatography (NP-HPLC) was used for preliminary separation of the additives, were compared. The additive...
-
Application of normal-phase high-performance liquid chromatography followed by gas chromatography for analytics of diesel fuel additives
Publikacja -
Size Exclusion Coupled to Reversed Phase Liquid Chromatography for the Characterization of Cranberry Products
PublikacjaA combination of solvent extraction, size exclusion purification, and reversed-phase liquid chromatography with UV absorption and fluorescence detection was here used for the determination of flavan-3-ols and related oligomeric compounds in cranberry products. The method relied on robust, simple, and inexpensive instrumental equipment present in most of routine analytical laboratories. Sample extracts in methanol/water/hydrochloric...
-
High resolution liquid chromatography and time of flight mass spectrometry in perfume analysis
PublikacjaPerfumes consist of a wide range of natural and synthetic compounds that belongs to different chemical classes. Most of these compounds are generally determined by GC. However, in this study RP-HPLC-Q-TOF-MS and HILIC-Q-TOF-MS technique was applied for the determination of ingredients of original perfumes and their imitations. Antioxidants and compounds specific to fragrances of animal \origin were found in original...
-
Determination of hormones in water samples using liquid chromatography coupled with tandem mass spectrometry
PublikacjaOver the past few years, many synthetic (ethinylestradiol) and natural (estradiol,estriol, estrone) compounds present in the environment have been affecting the hormonalbalance of humans and animals. Steroid hormones are very often used for medical purposesand have high estrogenic activity what makes them endocrine disrupters. Most hormonalcompounds are released into the water as a result of their incomplete removal in wastewatertreatment...
-
Polyfunctional chemically bonded stationary phase for reversed phase high-performance liquid chromatography
Publikacja -
Liquid chromatography tandem mass spectrometry study of urinary nucleosides as potential cancer markers
Publikacja -
Suppression of deleterious effects of free silanols in liquid chromatography by imidazolium tetrafluoroborate ionic liquids
Publikacja -
Comparative study of surface topography of high performance liquid chromatography columns in terms of hydrophobicity
Publikacja -
Characterization of In Vitro and In Vivo Metabolism of Antazoline Using Liquid Chromatography-Tandem Mass Spectrometry
Publikacja -
Troubleshooting of the determination of bisphenol A at ultra-trace levels by liquid chromatography and tandem mass spectrometry
PublikacjaDetermination of trace amounts of bisphenol A (BPA) may cause problems mainly related to the presence of BPA in solvents (even in LC-MS grade), laboratory vessels, and plastic equipment used for sample preparation. Variable and sometimes significant amounts of BPA present in the background cause problems in obtaining good repeatability of measurements at the ultra-trace levels. Such observations (i.e., poor repeatability of results)...
-
Optimal conditions of naphthoquinones separation from carnivorous plants extracts using thin-layer chromatography and high performance liquid chromatography
PublikacjaDroseraceae plants are owned by the family of carnivorous plants, which in an unusual way adapt to environments poor in nutrients. Droseraceae obtain essential nutrients catching insects and other small invertebrantes. Plumbagin, chloroplumbagin, ramentaceon and droseron are naphthoquinones, which could be found in leaves and shoots of plants from Droseraceae family. In the literature there are a lot of information about the pharmacological...
-
DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS IN SOIL SAMPLES USING ULTRASONIC PROBE AND SALT-ASSISTED LIQUID-LIQUID EXTRACTION COUPLED WITH HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
Publikacja -
“Dilute & Shoot” approach for rapid determination of trace amounts of nicotine in zero-level e-liquids by reversed phase liquid chromatography and hydrophilic interactions liquid chromatography coupled with tandem mass spectrometry-electrospray ionization
Publikacja -
“Dilute & Shoot” approach for rapid determination of trace amounts of nicotine in zero-level e-liquids by reversed phase liquid chromatography and hydrophilic interactions liquid chromatography coupled with tandem mass spectrometry-electrospray ionization
PublikacjaTwo analytical procedures are proposed where HILIC and RPLC techniques are coupled with tandem mass spectrometry detection for rapid determination of trace amounts of nicotine in zero-level liquids for electronic cigarettes. Samples are prepared on the basis of the approach “dilute & shoot” which makes this important step quick and not complicated. The chromatographic separation was carried out on a Zorbax XDB column (RPLC method)...
-
A research on the optimal conditions for separation and identyfication of the components from the Droseraceae extracts by column liquid chromatography
PublikacjaThe isolation of the components with anticipated biological properties i.e. antimicrobial, fungicidal, antioxidant and perhaps anticancer activity is for many years a one of the biggest issues of modern separation science. Their obtaining based on the isolation of the components with the biological activity from in vitro cultured plants by extraction techniques is often more difficult than their production by the chemical synthesis.The...
-
Comprehensive methodology for Staphylococcus aureus lipidomics by liquid chromatography and quadrupole time-of-flight mass spectrometry
PublikacjaStaphylococcus aureus is a common pathogen known to cause relatively minor infections as well as severe disorders in humans. Although there is fair amount of published data concerning various aspects of its biology, epidemiology, genetics, etc., there is still a scarce amount of data presenting reliable and thorough investigations regarding high-throughput analysis of total S. aureus lipid content. Therefore, the aim of this study...
-
Determination of isoprostanes in exhaled breath condensate samples by liquid chromatography coupled with tandem mass spectrometry
PublikacjaOxidative stress (OS) is a state of imbalance between continuously generating reactive oxygen species (ROS) during normal cell metabolism and mechanism of detoxifying oxygen radicals through a network of antioxidative enzymes. Isoprostanes are prostaglandin -like substances that are produced in vivo primarily by free radical-induced peroxidation of arachidonic acid. The measurement of isoprostanes in biological fluids and/or tissue...
-
Study of pH and temperature effect on lipophilicity of catechol-containing antioxidants by reversed phase liquid chromatography
PublikacjaLipophilicity of selected antioxidant phytochemicals, including flavonoids, phenolic acids and xanthonoids, was determined by reversed phase high performance liquid chromatography with UV detection (RP-HPLC-UV). The analyses run at different temperature and pH conditions in isocratic mode suggested that lipophilicity as distribution coefficient (logD) between aqueous and organic phase decreases with increasing temperature. For...
-
Determination of high-intensity sweeteners in beverages by high-performance liquid chromatography with mass spectrometry detection.
PublikacjaThe objective of the present study was to measure the concentration of nine high intensity sweeteners (acesulfame-K, aspartame, alitame, cyclamate, dulcin, neohesperidin dihydrochalcon, neotame, saccharin and sucralose) in different types of beverages available on the polish market. The proposed methodology reported here consists of a extraction with buffer composed of formic acid and N,N-diisopropylethylamine (pH 4.5) followed...
-
The application of gradient reversed-phase high-performance liquid chromatography to the pKa and determination of polyprotic analytes
Publikacja -
The lipophilicity indices of flavonoids estimated by reversed-phase liquid chromatography using different computation methods
PublikacjaDo oceny właściwości chromatograficznych wybranych flawonoidów zastosowano wyskokosprawną chromatografię cieczową z odwróconym układem faz i kolumnami chromatograficznymi typu C-8 i C-18.Lipofilowość wyrażono za pomocą różnych deskryptów lipofilowości.
-
Lipophilicity data for some preservatives estimated by reversed-phase liquid chromatography and different copmutation methods
PublikacjaW pracy przedstawiono wyniki badań właściwości chromatograficznych wybranych 12 związków z grupy kopnserwantów. Lipofilowy charakter tych związków był oceniany na podtsawie różnych parametrów retencyjnych (log Kw, log K i inne. Zastosowano narzędzie chemometryczne do zbadania korelacji występujących pomiędzy tymi parametrami retencji chromatogfraficznej.
-
High-performance liquid chromatography in group-type separation and technical or process analytics of petroleum products
PublikacjaW pracy dokonano przeglądu i poddano krytycznej ocenie metody oznaczania składu grupowego produktów naftowych o różnej lotności, zarówno metody znormalizowane, jak i opisane w literaturze naukowej. W zdecydowanej większości są to metody wykorzystujące technikę chromatografii cieczowej w układzie faz normalnych (NP-HPLC). Na podstawie danych z literatury oraz własnych studiów i badań należy stwierdzić, że im wyższy zakres temperatury...
-
Uncertainty of antioxidant profiling in complex mixtures using liquid chromatography involving post-column derivatisation
PublikacjaThe main goal of this paper is to discuss the problems associated with antioxidant profiling in complex samples using a high-throughput HPLC system coupled with post-column derivatisation reactor. Based on the experimental data reported in the literature, we demonstrated that improper optimisation of temperature and/or pH assay conditions performed using an on-line derivatisation reactor may substantially change the antioxidant...
-
Sensitive determination of isoprostanes in exhaled breath condensate samples with use of liquid chromatography-tandem mass spectrometry
PublikacjaOxidative stress is the hallmark of various inflammatory lung diseases. Increased concentrations of reactive oxygen species in the lungs are reflected by elevated concentrations of oxidative stress markers in the breath, airways, lung tissue and blood. The aim of this work was to develop a method for the fast measurement of F2 isoprostanes in exhaled breath condensate (EBC) samples using equipment which is nowadays available and...
-
Comparison of High Performance Liquid Chromatography Methods with Different Detectors for Determination of Steroid Hormones in Aqueous Matrices
PublikacjaThis paper presents the development and comparison of procedures for the qualitative and quantitative determination of five estrogenic compounds (17-a-ethynylestradiol, estrone, estradiol, estriol, and progesterone) in drinking water and wastewater samples. Five extraction columns and two disks were tested for their efficiency. The C18 columns were superior on the basis of cost for solid phase extraction of drinking water or sewage....
-
Sensitive determination of isoprostanes in exhaled breath condensate samples with use of liquid chromatography–tandem mass spectrometry
Publikacja -
Retention time and peak width in the combined pH/organic modifier gradient high performance liquid chromatography
Publikacja -
Effect of sample-preparation methods on the quantification of selected flavonoids in plant materials by high performance liquid chromatography
Publikacja -
Determination of nine high-intensity sweeteners in various foods by high-performance liquid chromatography with mass spectrometric detection
PublikacjaAn analytical procedure involving solid-phase extraction (SPE) and high-performance liquid chromatography-mass spectrometry has been developed for the determination of nine high-intensity sweeteners authorised in the EU; acesulfame-K (ACS-K), aspartame (ASP), alitame (ALI), cyclamate (CYC), dulcin (DUL), neohesperidin dihydrochalcone (NHDC), neotame (NEO), saccharin (SAC) and sucralose (SCL) in a variety of food samples (i.e. beverages,...
-
Analysis of multi-class pharmaceuticals in fish tissues by ultra-high-performance liquid chromatography tandem mass spectrometry
PublikacjaA new sensitive method based on pressurized liquid extraction (PLE) and purification by gel permeation chromatography (GPC) prior to ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC–MS/MS) was developed for the determination in fish homogenate, liver and muscle of twenty pharmaceuticals compounds and metabolites from seven commonly used therapeutic families. An extensive matrix effect evaluation...
-
Development of a liquid chromatography–tandem mass spectrometryprocedure for determination of endocrine disrupting compounds infish from Mediterranean rivers
PublikacjaA new, sensitive and rapid method based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe)approach followed by ultra high performance liquid chromatography coupled with tandem mass spec-trometry (UHPLC–MS/MS) was developed for the determination of nineteen endocrine disruptors (EDCs)and related compounds belonging to different classes in various fish species. Matrix effect on the analyti-cal performance was evaluated,...
-
Determination of tocopherols and tocotrienols in human breast adipose tissue with the use of high performance liquid chromatography-fluorescence detection
Publikacja -
Process control of leakages in refinery reforming plant by means of Normal Phase High Performance Liquid Chromatography (HPLC)
PublikacjaPraca dotyczy zbadania możliwości, opracowania nowej procedury badawczej oraz dokonania oznaczeń ¬m-krezolu w strumieniu produktu z procesu reformingu katalitycznego techniką wysokosprawnej chromatografii cieczowej w układzie faz normalnych, z detektorem spektrofotometrycznym typu DAD (NP-HPLC-UV-VIS/DAD). Opracowana procedura stanowi alternatywę dla innych procedur wykorzystujących klasyczne techniki analityczne, albo wykorzystanie...
-
Determination of flavanols by liquid chromatography with fluorescence detection. Application to the characterization of cranberry-based pharmaceuticals through profiling and fingerprinting approaches
PublikacjaIn this work, a new method based on reversed-phase high-performance liquid chromatography (HPLC) with fluorescence detection (FLD) was established for the determination of catechins and related oligomeric proanthocyanidins (PACs) in cranberry-based pharmaceuticals. Compounds were recovered by liquid extraction using methanol/water/hydrochloric acid (60:39:1, v:v:v) as the extraction solvent. The chromatographic separation was carried...
-
An evaluation of sucrose as a possible contaminant in e-liquids for electronic cigarettes by hydrophilic interaction liquid chromatography–tandem mass spectrometry
PublikacjaThe influence of sucrose combustion products on smoking and nicotine addiction is still controversial because the presence of the sucrose may be treated as a source of aldehydes and organic acids. In e-liquids used as refills for electronic cigarettes, which are made primarily of poly(propylene glycol), glycerine and ethanol, sucrose may be present at trace levels, and its impact on mainstream smoke formation, and hence on human...
-
Determination of Pesticide Residues in Honeybees using Modified QUEChERS Sample Work-Up and Liquid Chromatography-Tandem Mass Spectrometry
PublikacjaIncreasing emissions of chemical compounds to the environment, especially of pesticides, is one of factors that may explain present honeybee colony losses. In this work, an analytical method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) was optimized for the simultaneous screening of 19 pesticides which have not been yet determined in honeybee samples from northern Poland (Pomerania). The sample preparation,...
-
An evaluation of sucrose as a possible contaminant in e-liquids for electronic cigarettes by hydrophilic interaction liquid chromatography–tandem mass spectrometry
Publikacja -
Determination of Nine Intense Sweeteners in Foodstuffs by High-Performance Liquid Chromatography and Evaporative Light-Scattering Detection: Interlaboratory Study
PublikacjaW pracy przedstawiono wyniki badań międzylaboratoryjnych, opracowanej wcześniej metody jednoczesnego oznaczania dziewięciu słodzików o wysokiej mocy w produktach żywnościowych. W badaniach wzięło udział siedem laboratoriów (Belgia, Niemcy, Portugalia). W rezultacie dowiedziono, iż opracowana metoda umożliwia uzyskanie dokładnych, powtarzalnych i odtwarzalnych wyników analiz dla wszystkich badanych słodzików i typów żywności.
-
A Fast and Validated Method for the Determination of Malondialdehyde in Fish Liver Using High-Performance Liquid Chromatography with a Photodiode Array Detector
Publikacja -
Partition coefficients of selected environmentally important volatile organic compounds determined by gas - liquid chromatography with polydimethylsiloxane stationary phase
PublikacjaPrzedstawiono wartości współczynników podziału (stałych podziału) 14 lotnych związków organicznych istotnych dla stanu środowiska (często oznaczanych w powietrzu w pomieszczeniach zamkniętych) między fazą gazową (N2) a ciekłą (PDMS). Wyznaczano je dwiema technikami (izotermiczną i z liniowo programowanym wzrostem temperatury). Dowiedziono, że istnieje możliwość wyznaczania współczynników podziału techniką LTPRI w oparciu o skalę...
-
Determination of nonsteroidal antiinflammatory drugs in water samples using liquid chromatography coupled with diode-array detector and mass spectrometry
PublikacjaOpracowano i zwalidowano metodykę do oznaczania śladowych ilości sześciu różnych związków z grupy niesteroidowych leków przeciwzapalnych w próbkach wodnych. Anality zostały wybrane spośród najczęsciej sprzedawanych leków w Polsce. Sprawdzono kilka różnych kolumienek ekstrakcyjnych i wybrano RP-18 jako najlepsze wypełnienie do wzbogacania oznaczanych związków. Opracowaną metodykę z powodzeniem zastosowano do analizy próbek rzeczywistych.
-
Direct injection liquid chromatography-mass spectrometry (DI-LC-MS) analysis for rapid lipidomic profiling of extracellular vesicles
PublikacjaExtracellular vesicles (EVs) are small, spherical particles produced by eukaryotic and prokaryotic cells, surrounded by a bilayer membrane and carrying various bioactive molecules, such as proteins, surface receptors, membrane and soluble proteins, lipids, and nucleic acids. EVs are of substantial interest because of their important roles in cell communication, epigenetic regulation and possible application in disease diagnosis...
-
Application of dispersive liquid-liquid microextraction for the determination of selected organochlorine pesticides in honey by gas chromatography - mass spectrometry
PublikacjaDispersive liquid-liquid microextraction (DLLME) is a rapid and easy technique that consumes minute amounts of organic solvents. In this work, we present chemometric study on optimization of DLLME parameters for the extraction of aldrin, endrin, lindane, alfa-endosulfan, 4,4'-DDT and its metabolites from honey matrix. Method quantification limits (MQLs) vary between 0.3 ng/g for 2,4'-DDE and 4,4'-DDE to 13.2 ng/g for alfa-endosulfan...
-
Comparison of classical and modern approach of determination of highly polar compounds by High Performance Liquid Chromatography coupled with Tandem Mass Spectrometry
PublikacjaAbstract: Sugars and sugar alcohols are known as highly polar compounds. Four analytes were taken into consideration for comparison of classical and modern approach of separation of highly polar substances. Mannitol was chosen as an example of simple sugar alcohol, sucrose and lactulose as examples of disaccharides and structural isomers, raffinose as an example of complex trisaccharide. Raffinose was also used as internal standard...
-
Measurement of activity coefficients at infinite dilution of organic solutes in the ionic liquid 1-hexyl-1,4-diaza[2.2.2]bicyclooctanium bis(trifluoromethylsulfonyl)imide using gas–liquid chromatography
PublikacjaBased on gas–liquid chromatography (GLC) retention data, activity coefficients at infinite dilution of 39 different solutes in the ionic liquid 1-hexyl-1,4-diaza[2.2.2]bicyclooctanium bis(trifluoromethylsulfonyl)imide ([HDABCO][NTf2]) have been determined. Measurements covered a broad range of types of organic solutes including alkanes, alcohols, ketones, benzene derivatives and McReynold’s compounds. Activity coefficients were...
-
Determination of trace levels of eleven bisphenol A analogues in human blood serum by high performance liquid chromatography–tandem mass spectrometry
PublikacjaChemicals showing structural or functional similarity to bisphenol A (BPA), commonly called BPA analogues, have recently drawn scientific attention due to their common industrial and commercial application as a substitute for BPA. In the European Union, the use of BPA has been severely restricted by law due to its endocrine disrupting properties. Unfortunately, it seems that all BPA analogues show comparable biological activity,...
-
Rapid Characterization of the Human Breast Milk Lipidome Using a Solid-Phase Microextraction and Liquid Chromatography-Mass Spectrometry-Based Approach.
PublikacjaHuman breast milk (HBM) is a biofluid consisting of various biomolecules such as proteins, lipids, carbohydrates, minerals and bioactive substances. Due to its unique and complex composition, HBM provides not only nutritional components required for the growth of the infant, but also additional protection against infections. Global insight into the composition of HBM is crucial to understanding the health benefits infants receive...
-
Discrepancies in determination of biogenic amines in beer samples by reversed phase and hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry
PublikacjaBiogenic amines (BAs) are nitrogenous organic bases occurring mainly in fermented food and beverages as a result of free amino acids bacterial decarboxylation. The reversed phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) based methods were compared in terms of usefulness for determination of BAs in beer samples. Analysis of BAs with the use of RPLC method were carried out after their...
-
Determination of Butyltin Species in Sea Water Samples Using Solid Phase Extraction Followed by Liquid Chromatography - Tandem Mass Spectrometry
PublikacjaReverse phase liquid chromatography (RP-HPLC)-electrospray ionization-tandem mass spectrometry (ESI-MS-MS) was evaluated for the analysis of TBT using solid phase extraction as sample preparation method. TBT was extracted from acidified water samples (spiked with tripropyltin as internal standard) using reversed phase polymeric Strata-X cartridges. Analytes were eluted from cartridges using acetonitrile-water mixture and the volume...
-
Identification and characterization of the Indian Yellow dyestuff and its degradation products in historical oil paint tube by liquid chromatography mass spectrometry
PublikacjaAn analytical protocol for identification of dyes using reversed phase liquid chromatography-mass spectrometry with atmospheric pressure electrospray ionization (LC-ESI/MS) is presented. The developed method has been successfully applied in identification of euxanthic acid and euxanthone, the main components of the Indian Yellow dye in a historical oil paint tube produced by Richard Ain`es, a recognizable French company supplying...
-
Analysis of chiral pharmaceutical residues in influent and effluent samples at racemic and enantiomeric level using liquid chromatography-tandem mass spectrometry
PublikacjaIn this work, two different chromatographic methods for seasonal monitoring of pharmaceutical residue in wastewater samples were developed. In the case of enantiomeric separation of selected compounds, LC-MS technique combining with vancomycin based chiral stationary phase was used. The performance of chiral analysis enabled to monitor the pharmaceutical contamination at the enantiomeric level. The d-SPE procedure was developed...
-
Separation of Monovinyl and Divinyl Protochlorophyllides Using C30 Reverse Phase High Performance Liquid Chromatography Column: Analytical and Preparative Applications
Publikacja -
Quantification of DNA Modifications Using Two-Dimensional Ultraperformance Liquid Chromatography Tandem Mass Spectrometry (2D-UPLC-MS/MS)
Publikacja -
Analysis of serum homocysteine in the laboratory practice - comparison of the direct chemiluminescence immunoassay and high performance liquid chromatography coupled with fluorescent detection
Publikacja -
The efficiency comparison of ramentaceone isolation from Drosera aliciae plants using normal and reversed phase preparative liquid chromatography technique
PublikacjaW pracy zaprezentowano optymalne warunki otrzymywania ramentaceonu z chloroformowego ekstraktu z rośliny D. muscipula. Opracowano i przedstawiono dwie procedury otrzymywania ramantaceonu z wykorzystaniem NP-PLC i RP-PLC. Dla produktu o określonej czystości, porównano maksymalny stopień przeładowania kolumny, efektywność procesu wyrażoną jako ilość substancji otrzymanej w jednostce czasu oraz produktywność kolumny, wyrażoną jako...
-
Steroid profiles as potential biomarkers in patients with urogenital tract cancer for diagnostic investigations analyzed by liquid chromatography coupled to mass spectrometry
Publikacja -
Molecularly imprinted stir bar sorptive extraction coupled with high-performance liquid chromatography for trace analysis of naphthalene sulfonates in seawater
Publikacja -
Sensitive simultaneous determination of 19 fluorobenzoic acids in saline waters by solid-phase extraction and liquid chromatography–tandem mass spectrometry
PublikacjaA solid-phase extraction (SPE) procedure using C18 stationary phase was optimized for the preconcentration of 19 fluorinated derivatives of benzoic acid (FBA): mono-, di-, tri-, and tetrafluorosubstituted in the ring, trifluoromethylbenzoic acid and 3,5-bistrifluoromethyl benzoic acid from undiluted salt-rich (>20%) reservoir waters. Quantitative (>90%) retention/elution of 16 out of 19 analyte compounds was achieved allowing a...
-
Simultaneous Determination of Indolic Compounds in Plant Extracts by Solid-Phase Extraction and High-Performance Liquid Chromatography with UV and Fluorescence Detection
PublikacjaA high-performance liquid chromatographic method with UV and fluorescence detection (HPLC-DAD-FLD) was developed for simultaneous determination of indolic compounds in plant material. Indole-3-carbinol (I3C), indole-3-acetic acid (I3AA), indole-3-acetonitrile (I3ACN), and 3,3′-diindolylmethane (DIM) were used as representative compounds that cover a wide spectrum of indole structures occurring in nature. For concentration and purification...
-
Determination of robenidine in animal feeds by liquid chromatography coupled with diode-array detection and mass spectrometry after accelerated solvent extraction
PublikacjaRobenidyna (chlorowodorek 1,3-bis[(4-chlorobenzylideno)amino]guanidyny) jest stosowana w celu zapobiegania i leczenia kokcydiozy - pasożytniczej choroby układu pokarmowego wielu zwierząt hodowlanych (kozy, owce, króliki, drób) wywoływanej przez pierwotniaki należące do rzędu Eimeria tenella, E. Necatrix, E. Acervulina, E. Maxima, E. Brunetti, and E. Mivati. Robenidyna wykazuje stosunkowo wysoką skuteczność terapeutyczną, przy niewielkiej...
-
Procedure for and results of simultaneous determination of aromatic hydrocarbons and fatty acids methyl esters in diesel fuels by high performance liquid chromatography
PublikacjaW pracy przedstawiono taki sposób modyfikacji metody EN-12916 /ASTM D-6591, który zapewnia jednoczesne oznaczenie poszczególnych grup węglowodorów aromatycznych, całkowitej zawartości węglowodorów poliaromatycznych oraz zawartości FAME w oleju napędowym. Stosuje się nadal detektor refraktometryczny i elucję n-heptanem, jednak przepływ zwrotny eluentu jest wykonywany po elucji policyklicznych węglowodorów aromatycznych. Dodatkowo...
-
Determination of fuel combustion product in airport runoff water samples using liquid–liquid extraction with gas chromatography–spectrometry
PublikacjaDetermination of xenobiotics in samples of airport runoff water is both a complex and indispensable task due to an increasing threat resulting from the activities of numerous airports. The aim of this study was to develop, optimize, and validate a procedure based on liquid–liquid extraction (LLE) coupled with gas chromatography–mass spectrometry (GC–MS) for the determination of polycyclic aromatic hydrocarbons (PAHs). So far, no...
-
Comprehensive determination of flavouring additives and nicotine in e-cigarette refill solutions. Part I: Liquid chromatography-tandem mass spectrometry analysis
PublikacjaLiquid chromatography-tandem mass spectrometry with electrospray ionization (HPLC-ESI–MS/MS) methods were developed for the simultaneous determination of 42 flavouring compounds and nicotine in liquids for e-cigarettes. The chromatographic separation was performed using an Ace® Ultracore™ SuperC18™ (100 × 2.1 mm, 2.5 μm) column in both acidic and alkaline pH conditions to separate all the compounds. A simple “dilute & shoot” approach...
-
Headspace liquid phase microextraction for quantitation of hexanal in potato crisps by gas chromatography
Publikacja -
Profiling and Qualitative Assessment of Enzymatically and Thermally Oxidized Egg Yolk Phospholipids using a Two‐Step High‐Performance Liquid Chromatography Protocol
PublikacjaA two‐step high‐performance liquid chromatography (HPLC) method for the profiling and qualitative assessment of oxidized phospholipids (oxPL) present in foods was developed. The applicability of the investigated two‐step HPLC protocol was verified for separation of enzymatically and thermally oxidized hen egg yolk phospholipids (PL) as a relevant food model. In the first step, seven individual PL classes were separated using hydrophilic...
-
Application of high performance liquid chromatography with ultraviolet diode array detection and refractive index detection to the determination of class composition and the analysis of gasoline.
PublikacjaZaprezentowano nową metodę HPLC oznaczania składu grupowego benzyn i komponentów benzyn tj. oznaczania zawartości olefin, węglowodorów aromatycznych i parafinowo-naftenowych.
-
Determination of amikacin and ciprofloxacin by liquid chromatography with pre-column derivatization to evaluate sustained delivery of antibiotics from Drug-Eluting Biopsy Needle
PublikacjaDetermination of chosen antibacterial antibiotics: amikacin and ciprofloxacin was carried out by HPLC-UV after derivatization with 9-fluorenylmethyl chloroformate and in their native form by HPLC-MS/MS. Developed methods have been applied to control the kinetics of antibiotic release from polymer-based controlled drug delivery system.
-
A rapid dispersive liquid–liquid microextraction of antimicrobial onion organosulfur compounds in animal feed coupled to gas chromatography-mass spectrometry
PublikacjaA rapid analytical procedure is proposed for determining two antimicrobial onion organosulfur compounds, propyl disulfide (PDS) and propyl propane thiosulfonate (PTSO), in animal feed. The use of PTSO as a natural ingredient in animal feed is allowed due to its antimicrobial activity against pathogenic organisms. Two analytical methodologies using gas chromatography coupled to mass spectrometry (GC-MS) are compared. After the extraction...
-
Least absolute shrinkage and selection operator and dimensionality reduction techniques in quantitative structure retention relationship modeling of retention in hydrophilic interaction liquid chromatography
Publikacja -
Simultaneous determination of hydrophobicity and dissociation constant for a large set of compounds by gradient reverse phase high performance liquid chromatography–mass spectrometry technique
Publikacja -
Molecular mechanism of retention in reversed-phase high-performance liquid chromatography and classification of modern stationary phases by using quantitative structure–retention relationships
Publikacja -
Headspace Liquid-Phase Microextraction of Chlorobenzenes in Soil with Gas Chromatography-Electron Capture Detection
Publikacja -
Dispersive liquid-liquid microextraction combined with gas chromatography–mass spectrometry for in situ determination of biogenic amines in meat: Estimation of meat's freshness
PublikacjaA dispersive liquid-liquid microextraction (DLLME) gas chromatography–mass spectrometry (GC–MS) technique was developed for the determination of selected biogenic amines (BAs) in samples of poultry, pork and beef. Prior to the extraction process, an appropriate volume of sodium hydroxide solution was added to each of the portioned samples. Next, samples were homogenized, centrifuged and finally sonicated at an increased temperature....
-
Determination of phenylbutazone, sulfamethazine, carbendazim and linuron using a novel pine bark biosorbent for solid-phase extraction (SPE) with high-performance liquid chromatography (HPLC)
PublikacjaThe paper presents a novel pine bark based biosorbent for solid-phase extraction (SPE). The morphology and composition of the pine bark were characterized using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The biosorbent was used for the preparation of wastewater for the determination of phenylbutazone, sulfamethazine, carbendazim, and linuron by high performance liquid chromatography with...
-
Determination of bromhexine and its metabolites in equine serum samples by liquid chromatography – Tandem mass spectrometry: Applicability to the elimination study after single oral dose
PublikacjaBromhexine (BH), expectorant used in the treatment of respiratory disorders associated with viscid or excessive mucus, is not permitted for use in the competing horse by many authorities in horseracing and Olympic disciplines. Metabolic studies are of the great importance in anti-doping field because they allow for updating the selection of the most appropriate markers for prohibited substances, such as metabolites present at higher...
-
Optimal conditions of ramentaceone and plumbagin separation and isolation from carnivorous plants extracts using reverse phase-high performance liquid chromatography (RP-HPLC)
PublikacjaPreparatywna chromatografia cieczowa jest szczególnie przydatną techniką do rozdzielania i izolacji użytecznych ilości substancji z różnych skomplikowanych mieszanin. Praca prezentuje optymalne warunki rozdzielania i izolacji ramentaceonu i plumbaginy uwzględniając maksymalną selektywność rozdzielania naftochinonów od substancji balastowych z chloroformowych ekstraktów roślinnych D. aliciae i D. capensis z zastosowaniem techniki...
-
Optimal conditions of ramentaceone and plumbagin separation and isolation from carnivorous plants extracts using normal phase-high performance liquid chromatography (NP-HPLC)
PublikacjaPraca prezentuje optymalne warunki rozdzielania i izolacji plumbaginy i ramentacenu z matrycy analitycznej, którą stanowią chloroformowe ekstrakty z roślin D.aliciae i D.capensis z wykorzystaiem techniki NP-HPLC. Skład eluentu dla żelu krzemionkowego jako fazy stacjonarnej był optymalizowany. Zostały przedstawione optymalne warunki powiększania skali procesu izolacji badanych metabolitów z ekstrakrów roślinnych. Określono również...
-
Using pattern recognition entropy to select mass chromatograms to prepare total ion current chromatograms from raw liquid chromatography–mass spectrometry data
Publikacja -
Method for the determination of carboxylic acids in industrial effluents using dispersive liquid-liquid microextraction with injection port derivatization gas chromatography–mass spectrometry
PublikacjaThe paper presents a new method for the determination of 15 carboxylic acids in samples of postoxidative effluents from the production of petroleum bitumens using ion-pair dispersive liquid-liquid microextraction and gas chromatography coupled to mass spectrometry with injection port derivatization. Several parameters related to the extraction and derivatization efficiency were optimized. Under optimized experimental conditions,the...
-
Determination of eight artificial sweeteners and common Stevia rebaudiana glycosides in non-alcoholic and alcoholic beverages by reversed-phase liquid chromatography coupled with tandem mass spectrometry.
PublikacjaThe method for the determination of acesulfame-K, saccharine, cyclamate, aspartame, sucralose, alitame, neohesperidin dihydrochalcone, neotame and five common steviol glycosides (rebaudioside A, rebaudioside C, steviol, steviolbioside and stevioside) in soft and alcoholic beverages was developed using high-performance liquid chromatography and tandem mass spectrometry with electrospray ionisation (HPLC-ESI-MS/MS). To the best of...
-
Novel liquid chromatography method based on linear weighted regression for the fast determination of isoprostane isomers in plasma samples using sensitive tandem mass spectrometry detection
PublikacjaA simple, fast, sensitive and accurate methodology based on a LLE followed by liquid chromatography–tandem mass spectrometry for simultaneous determination of four regioisomers (8-iso prostaglandin F2α, 8-iso-15(R)-prostaglandin F2α, 11β-prostaglandin F2α, 15(R)-prostaglandin F2α) in routine analysis of human plasma samples was developed. Isoprostanes are stable products of arachidonic acid peroxidation and are regarded as the...
-
Ultrasound assisted solvent extraction of porous membrane-packed samples followed by liquid chromatography-tandem mass spectrometry for determination of BADGE, BFDGE and their derivatives in packed vegetables
PublikacjaThe problem of the presence of trace organic pollutants in food is of growing importance due to increasing awareness about their impact on newborns, infants and adults of reproductive age. Despite the fact that packaged food products offer many advantages, packaging can be a source of contamination for stored food. Thus, monitoring such pollution in food is of high importance. In this work, a novel methodology based on the solvent...
-
Stabilities of bisphenol A diglycidyl ether, bisphenol F diglycidyl ether, and their derivatives under controlled conditions analyzed using liquid chromatography coupled with tandem mass spectrometry
PublikacjaBisphenol A diglycidyl ether (BADGE), bisphenol F diglycydyl ether (BFDGE), and their related compounds are widely used as precursors in production of epoxy resins. The high reactivity of these compounds makes the development of analytical methodologies that ensure appropriate metrological accuracy crucial. Consequently, we aimed to determine whether and to what extent the composition of the solution and storage conditions affect...
-
Comparison of hydrophilic interaction and reversed phase liquid chromatography coupled with tandem mass spectrometry for the determination of eight artificial sweeteners and common steviol glycosides in popular beverages
PublikacjaHydrophilic interaction liquid chromatography (HILIC) coupled with tandem mass spectrometry (MS/MS) was used to separate artificial and natural sweeteners approved for use in European Union (EU). Among three tested HILIC columns (BlueOrchid PAL-HILIC, Ascentis Express Si and Acclaim™ Trinity™ P2) the last one was selected for the development of HILIC method due to the best results obtained with it. Early eluting and coeluting compounds...
-
Dispersive solid-phase extraction using multi-walled carbon nanotubes combined with liquid chromatography–mass spectrometry for the analysis of β-blockers: Experimental and theoretical studies
Publikacja -
Comparison of mouse plasma and brain tissue homogenate sample pretreatment methods prior to high-performance liquid chromatography for a new 1,2,4-triazole derivative with anticonvulsant activity
Publikacja -
An in situ derivatization – dispersive liquid–liquid microextraction combined with gas-chromatography – mass spectrometry for determining biogenic amines in home-made fermented alcoholic drinks
PublikacjaA novel dispersive liquid–liquid microextraction (DLLME) gas chromatography mass-spectrometry(GC–MS) method was developed for the determination of 13 biogenic amines in home-made wine sam-ples. The method allows to simultaneous extraction and derivatization of the amines providing a simpleand fast mode of extract enrichment. During the study, two different procedures were examined. Sta-tistical analysis was performed to choose...
-
Application of dispersive liquid-liquid microextraction and gas chromatography with mass spectrometry for the determination of oxygenated volatile organic compounds in effluents from the production of petroleum bitumen
PublikacjaWepresent a new procedure for the determination of oxygenated volatile organic compounds in samples of postoxidative effluents from the production of petroleum bitumens using dispersive liquid–liquid microextraction and gas chromatography with mass spectrometry. The eight extraction parameters were optimized for 43 oxygenated volatile organic compounds. The detection limits obtained ranged from 0.07 to 0.82 g/mL for most of the...
-
Common methods for the chiral determination of amphetamine and related compounds I. Gas, liquid and thin-layer chromatography
PublikacjaThis article reviews the most common, useful methods for the chiral determination of amphetamine (AM) and AM-deriveddesigner drugs in different of matrix, including blood, hair, urine, medicaments or standard solutions, taking into considerationarticles published in the past 15 years. We consider chromatographic methods (e.g., gas, liquid, high-performance liquid, and thinlayer). We describe several types of chiral derivatization...
-
Direct Screening ofWater Samples for Benzene Hydrocarbon Compounds by Headspace Liquid-Phase Microextraction--Gas Chromatography
Publikacja