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Wyniki wyszukiwania dla: MEMBRANE ERYTHROCYTE
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Nonlinear FEM analysis of irregular shells composed of fiber metal laminates
PublikacjaThe paper deals with the analysis of failure initiation in shells made of Fiber Metal Laminates (FML). The elas-tic material law for orthotropic lamina is stated accounting for asymmetric in-plane stress and strain measures. The asymmetry results from the employed general nonlinear 6-field shell theory where the generalized dis-placements involve the translation and the proper rotation field. The novelty of the presented results...
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Charakterystyka areodynamiczna membranowego przekrycia wiszącego.
PublikacjaArtykuł przedstawia charakterystykę aerodynamiczną membranowego przekrycia wiszącego. Prezentowane są dwie koncepcje przyjmowania wartości współczynnika aerodynamicznego. Pierwsza z koncepcji bazuje bezpośrednio na testach laboratoryjnych druga zaś wykorzystuje uproszczone podejście normowe.This paper presents the aerodynamic characteristics of membrane hanging roof. The two concepts of taking the value of the aerodynamic factor...
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Permeation and sorption properties of poly(ether-block-amide) membranesfilled by two types of zeolites
PublikacjaThe experimental study of transport properties of mixed matrix membranes (MMMs) based on PEBA-4033 with two types of zeolites is reported. Gas (CO2, O2, N2) and vapor (benzene, toluene, methanol, water) permeation measurements were performed using the differential flow permeameter at temperature of 298.15 K and at atmospheric pressure. Gas/vapor permeation was monitored by measuring the changes of the thermal conductivity of carrier...
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A Flexible Nafion Coated Enzyme‐free Glucose Sensor Based on Au‐dimpled Ti Structures
PublikacjaThe detection of glucose at low concentrations using electrochemical sensors is of great importance due to the possibility of using different human body fluids than blood, such as e.g. urine, saliva, sweat or tears. The interest behind those biofluids is related to their utility in non-invasive sugar determination. In this work, we present flexible, fully biocompatible electrode material based on Au nanoparticles immobilized onto...
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Regulated Assembly of LPS, Its Structural Alterations and Cellular Response to LPS Defects
PublikacjaDistinguishing feature of the outer membrane (OM) of Gram-negative bacteria is its asymmetry due to the presence of lipopolysaccharide (LPS) in the outer leaflet of the OM and phospholipids in the inner leaflet. Recent studies have revealed the existence of regulatory controls that ensure a balanced biosynthesis of LPS and phospholipids, both of which are essential for bacterial viability. LPS provides the essential permeability...
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Investigation of poly(3,4-ethylenedioxythiophene) deposition method influence on properties of ion-selective electrodes based on bis(benzo-15-crown-5) derivatives
PublikacjaGlassy carbon electrodes modified by conductive polymers and membrane with derivatives of bis(benzo-15-crown-5) were tested as solid contact ion selective electrodes for K+ ions concentration determination. PEDOT with PSS, Cl- and ClO4- counter ions was electrochemically deposited onto glassy carbon substrates using four different electrochemical approaches (potentiostatic, galvanostatic, potentiodynamic and potentiostatic pulses)....
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Dissection of σE‐dependent cell lysis in Escherichia coli: roles of RpoE regulators RseA, RseB and periplasmic folding catalyst PpiD
PublikacjaTo understand the mechanism of sigma(E)-dependent cell lysis, we examined the consequences of deletion derivatives of rpoE regulators rseA, rseB and rseC on sigma(E) transcription, on levels of free versus membrane-bound sigma(E) and on OMP-biogenesis limiting factor(s) that could impact cell lysis. RT-PCR showed that individual nonpolar DeltarseA and DeltarseB increased the rpoE expression to varying extents, with pronounced induction...
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Does the chemical modification of Nystatin A1 affect the drug's ability to overcome the multidrug resistance of fungi?
PublikacjaAlthough the contemporary medicine keeps moving forward, disseminated infections caused by fungal pathogens are an emerging challenge. The dramatic rise of fungal diseases, especially the most life-threatening systemic mycoses is associated with a permanently growing number of immunodeficient patients. Undoubted difficulties in the treatment of fungal infections are caused by lack of highly effective and selective antifungal drugs,...
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Electrochemical sensor for measurement of volatile organic compounds employing square wave perturbation voltage
PublikacjaThe paper presents the results of investigation on a prototype sensor for measurement of benzaldehyde in air. Sensitivity and limit of quantification of the sensor were determined for different internal electrolyte using the square wave voltammetry (SWV) as a detection technique. The working and counter electrodes were made of platinum. Ionic liquids 1-hexyl, 3-methylimidazolium chloride,1-hexyl, 3-methylimidazolium bis (trifluoromethanesulfonyl)...
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Local atomic order in low Pt-content nanocatalysts investigated in situ by XAS
PublikacjaThe unique features of X-ray absorption spectroscopy allow investigations of nanosized catalysts for fuel cells under working conditions. We present the results of an experiment carried out on a low Pt content electrocatalyst supported by a mesoporous heteropolyacid salt and used at the cathode of a proton exchange membrane fuel cell (PEMFC). The analysis of the EXAFS signal at the Pt L3-edge indicates that upon operating the fuel...
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PTD4 Peptide Increases Neural Viability in an In Vitro Model of Acute Ischemic Stroke
PublikacjaIschemic stroke is a disturbance in cerebral blood flow caused by brain tissue ischemia and hypoxia. We optimized a multifactorial in vitro model of acute ischemic stroke using rat primary neural cultures. This model was exploited to investigate the pro-viable activity of cell-penetrating peptides: arginine-rich Tat(49–57)-NH2 (R49KKRRQRRR57-amide) and its less basic analogue, PTD4 (Y47ARAAARQARA57-amide). Our model included glucose...
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Torsional elasticity and energetics of F1-ATPase
PublikacjaFoF1-ATPase is a rotary motor protein synthesizing ATP from ADP driven by a cross-membrane proton gradient. The proton flow through the membrane-embedded Fo generates the rotary torque that drives the rotation of the asymmetric shaft of F1. Mechanical energy of the rotating shaft is used by the F1 catalytic subunit to synthesize ATP. It was suggested that elastic power transmission with transient storage of energy in some compliant...
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Molecular Strategy for Survival at a Critical High Temperature in Eschierichia coli
PublikacjaThe molecular mechanism supporting survival at a critical high temperature (CHT) in Escherichia coli was investigated. Genome-wide screening with a single-gene knockout library provided a list of genes indispensable for growth at 47°C, called thermotolerant genes. Genes for which expression was affected by exposure to CHT were identified by DNA chip analysis. Unexpectedly, the former contents did not overlap with the latter except...
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Checkpoints that regulate balanced biosynthesis of lipopolysaccharide and its essentiality in Escherichia coli
PublikacjaThe outer membrane (OM) of Gram-negative bacteria, such as Escherichia coli, is essential for their viability. Lipopolysaccharide (LPS) constitutes the major component of OM, providing the permeability barrier, and a tight balance exists between LPS and phospholipids amounts as both of these essential components use a common metabolic precursor. Hence, checkpoints are in place, right from the regulation of the first committed step...
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Feasibility study of a Raman spectroscopic route to drug detection
PublikacjaWe present an surface-enhanced Raman spectroscopy (SERS) approach for detection of drugs of abuse in whole human blood. We utilize a near infrared laser with 830 nm excitation wavelength in order to reduce the influence of fluorescence on the spectra of blood. However, regular plasmon resonance peak of plasmonic nanoparticles, such as silver or gold fall in a much lower wavelength regime about 400 nm. Therefore, we have shifted...
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Direct injection liquid chromatography-mass spectrometry (DI-LC-MS) analysis for rapid lipidomic profiling of extracellular vesicles
PublikacjaExtracellular vesicles (EVs) are small, spherical particles produced by eukaryotic and prokaryotic cells, surrounded by a bilayer membrane and carrying various bioactive molecules, such as proteins, surface receptors, membrane and soluble proteins, lipids, and nucleic acids. EVs are of substantial interest because of their important roles in cell communication, epigenetic regulation and possible application in disease diagnosis...
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Badania parametrów metrologicznych amperometrycznego czujnika ditlenku siarki
PublikacjaW pracy porównano właściwości dwóch nowych prototypowych wersji amperometrycznego czujnika ditlenku siarki, wyposażonego w elektrody robocze wykonane w postaci warstw złota i platyny napylonych bezpośrednio na zewnętrzną powierzchnię membrany nafionowej. Elektrolitami wewnętrznymi były wodne roztwory kwasu siarkowego z dodatkiem dimetylosulfotlenku.
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Lower rim substituted p-tert-butyl-calix[4]arene. Part 17. synthesis, extractive and ionophoric properties of p-tert-butylcalix[4]arene appended with hydroxamix acid moieties
PublikacjaThe synthesis and characterization of four p-tert-butylcalix[4]arene-hydroxamic acidc are reported. The dependence of the metal ion binding, assessed by liquid extraction of the metal nitrates from water into dichloromethane in individual and competitive experiments, on the ligand structure, is presented. The results showed that those ligands could be successfully used in separation process of trnasition and heavy metals often...
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Modeling of subarachnoid space width change caused by blood circulation in brain vessels
PublikacjaThe subarachnoid space is an anatomic space, lying in the central nervous system, between the arachnoid membrane and the pia mater. It is filled with cerebrospinal fluid, which protects and nourishes the brain. The pathological disorder of the subarachnoid space (i.e. increase or decrease of its width) a ects the nervous system. Therefore, it is important to diagnose the width changes of the subarachnoid space. One way to support...
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Iron(III)-selective materials based on a catechol-bearing amide for optical sensing
PublikacjaThe synthesis and ion-binding properties of a new amide L derived from 3,4-dihydroxybenzoic acid are described. Due to the presence of a catechol unit, the compound interacts selectively with iron(III) in organic solvent (dimethyl sulfoxide, DMSO) to produce a color change from pale yellow to green. The incorporation of the ligand L into polymeric matrices or its encapsulation into surfactant-based spheres enables analyte detection...
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Functionalized azobenzocrown ethers as sensor materials—The synthesis and ion binding properties
PublikacjaNew 13- and 16-membered azobenzocrown ethers with aromatic amino, amide, ether–ester or ether–amide residue in para position to an azo moiety were obtained. Acid–base properties and ion binding ability of the colored compounds were studied by spectroscopic methods: UV–vis, fluorimetry and 1H NMR spectroscopy. Selected azobenzocrowns were tested as ionophores in ion-selective membrane electrodes (ISEs) – classic and miniature all...
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Green porous benzamide-like nanomembranes for hazardous cations detection, separation, and concentration adjustment
PublikacjaGreen biomaterials play a crucial role in the diagnosis and treatment of diseases as well as health-related problem-solving. Typically, biocompatibility, biodegradability, and mechanical strength are requirements centered on biomaterial engineering. However, in-hospital therapeutics require an elaborated synthesis of hybrid and complex nanomaterials capable of mimicking cellular behavior. Accumulation of hazardous cations like...
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Lower rim substituted tert-butylcalix[4]arenes (Part VI). Synthesis and ionophoric properties of 5,11,17,23-tetra-tert-butyl-25,26,27,28-tetrakis(3-diethoxyphosphorylpropoxy)calix[4]arene.
PublikacjaSyntezowano nowy związek: 5,11,17,23-tetra-tert-butylo-25,26,27,28-tetrakis(3-dietoksyfosforylopropoksy)kaliks[4]aren i badano jego właściwości kompleksujące w membranowych elektrodach jonoselektywnych. Związek wykazuje selektywność na jony wapnia oraz na kationy litu. Wyznaczono stałe tworzenia kompleksów z wymienionymi jonami w membranie elektrody.
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Microcapsules and their applications in pharmaceutical and food industry.
PublikacjaMicroencapsulation is a process in which tiny particles or droplets are surrounded by a coating or embedded in a homogeneous or heterogeneous matrix, to generate small capsules in size range from a few to few thousand micrometers. The material inside the microcapsule is referred to as the core, internal phase, or fill, whereas the wall is sometimes called shell, coating, wall material, or membrane. It has found application in...
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Minimal surfaces and conservation laws for bidimensional structures
PublikacjaWe discuss conservation laws for thin structures which could be modeled as a material minimal surface, i.e., a surface with zero mean curvatures. The models of an elastic membrane and micropolar (six-parameter) shell undergoing finite deformations are considered. We show that for a minimal surface, it is possible to formulate a conservation law similar to three-dimensional non-linear elasticity. It brings us a path-independent...
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Generalized Taylor formula and shell structures for the analysis of the interaction between geosythetics and engineering structures of transportation lines
PublikacjaThe analysis of the interaction between geosynthetics and engineering structures (e.g. railroad bed, soil foundation, pipeline) assumes that the geosynthetic form elastic membranes or shell laid on different types of foundations. The mathematical description of that problems in continuous domain employs Laplace operator Δ or d’Alembert operator □. In this paper we demonstrate the analysis based on generalize Taylor formula, and...
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Analytical characteristics of a new amperometric sulfur dioxide sensor
PublikacjaZaprojektowano i skonstruowano wyposażony w układ trójelektrodowy amperometryczny czujnik ditlenku siarki. Elektroda pracująca czujnika była napylona bezpośrednio na powierzchnię membrany. Zbadano charakterystyki czujnika z membranami z mikroporowatego Teflonu oraz Nafionu. Porównano parametry statyczne i dynamiczne tych czujników wypełnionych roztworami elektrolitów wewnętrznych, zawierających dodatek dimetylosulfotlenku.
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Removal of indicator bacteria from treated wastewater using physical and chemical methods. Pilot plant study.
PublikacjaEven after highly efficient biological processes the effluents from wastewater treatment plants may contain significant number of fecal bacteria, from 104 to 106 CFU/100 mL. Since no standards are set on the bacteriological quality of treated wastewater in Poland the elimination of bacterial contaminants is not a priority in wastewater treatment policy. Thus microbiological hazards become particularly significant in the situation...
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A BODIPY‐Based Molecular Rotor in Giant Unilamellar Vesicles: A Case Study by Polarization‐Resolved Time‐Resolved Emission and Transient Absorption Spectroscopy
PublikacjaBODIPY and BODIPY-derived systems are widely applied as fluorophores and as probes for viscosity detection in solvents and biological media. Their orientational and rotational dynamics in biological media are thus of vital mechanistic importance and extensively investigated. In this contribution, polarization-resolved confocal microscopy is used to determine the orientation of an amphiphilic BODIPY-cholesterol derivative in homogeneous...
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Biomechanical properties of 3D-printed bone models
PublikacjaBone lesions resulting from large traumas or cancer resections can be successfully treated by directly using synthetic materials or in combination with tissue engineering methods (hybrid). Synthetic or hybrid materials combined with bone tissue’s natural ability for regeneration and biological adaptation to the directions of loading, allow for full recovery of its biological functions. Increasing interest in new production methods...
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Chromogenic macrocyclic derivatives of azoles - synthesis and properties,
PublikacjaNew azocrown ethers comprising imidazoles in the macrocycle have been synthesized. Imidazole, 2-methyl-, 4-methyl-, and 4-phenylimidazole were incorporated to form macrocyclic units by coupling with the appropriate bis-diazonium salts. The syntheseswere performed under high dilution conditions. The X-ray structure of a water adduct of the 21-membered crown ether derivative of 4-methylimidazole 8 has been solved. Metal...
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Azomacrocyclic derivatives of imidazole: synthesis, structure, and metal ion complexation properties
PublikacjaNew azocrown ethers comprising imidazoles in the macrocycle have been synthesized. Imidazole, 2-methyl-, 4-methyl-, and 4-phenylimidazole were incorporated to form macrocyclic units by coupling with the appropriate bis-diazonium salts. The syntheseswere performed under high dilution conditions. The X-ray structure of a water adduct of the 21-membered crown ether derivative of 4-methylimidazole 8 has been solved. Metal...
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Impedance investigations of amperometric gas sensor containing aprotic solvent
PublikacjaSkonstruowany został amperometryczny czujnik gazowy w układzie trójelektrodowym ze złotą elektrodą roboczą bezpośrednio napyloną na membranę Nafionową. W publikacji przedstawiono wyniki badań charakterystyk czujnika do oznaczania ditlenku siarki wypełnionego elektrolitem zawierającym różne względne zawartości DMSO/H2O. Wyniki badań impedancyjnych zostały przeanalizowane w oparciu o zaproponowany elektryczny układ zastępczy.
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PVA-Based Films with Strontium Titanate Nanoparticles Dedicated to Wound Dressing Application
PublikacjaBioactive materials may be applied in tissue regeneration, and an example of such materials are wound dressings, which are used to accelerate skin healing, especially after trauma. Here, we proposed a novel dressing enriched by a bioactive component. The aim of our study was to prepare and characterize poly(vinyl alcohol) films modified with strontium titanate nanoparticles. The physicochemical properties of films were studied,...
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On aspects of tensile structures analysis.
PublikacjaW pracy dokonano omówienia podstawowych aspektów analizy konstrukcji napiętych. Przedstawiono krótki rys historyczny tego typu konstrukcji, zwracając uwagę m.in. na konstrukcje membranowe, cięgnowe, pneumatyczne.
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Hydrogen degradation of high-strength weldable steels in sea-water
PublikacjaW artykule przedstawiono ocenę podatności stali o wysokiej wytrzymałości oraz ich złączy spawanych na kruchość wodorową. Wykonano próby powolnego rozciągania na stalach gatunku 17HMBVA i 14HNMBCu oraz ich złączach spawanych, w środowisku wody morskiej, przy polaryzacji katodowej. Wykonano badania elektrochemiczne przenikania wodoru przez membrany pobrane z rożnych obszarów złączy spawanych.
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Utilization of ionic liquids for the enrichment of biogas
PublikacjaThis article discusses the dependence of biogas composition on organic feed material. High amounts of CO2 decrease the calorific value of biogas. Several technologies are reviewed which can be used to increase the calorific value. Special emphasis is laid on ionic liquids as a new absorbing material. ILs have a number of advantages due to their unique properties such as: low melting point, high thermal conductivity, no flammability,...
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Potentiometric Solid-Contact Multisensor System for Simultaneous Measurement of Several Ions
PublikacjaThe design and performance of potentiometric multisensor system made in solid-contact technology is described. Both, the measuring electrodes as well as the reference electrode are in a compact cell. The system allows for simultaneous measurement of various ions. The characteristics of the electrodes for Na+, K+ and Ca2+ ions were determined using the designed system and compared with ISEs of the same membrane composition in classical...
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Mechanical compatibility of implants used in hernia repair with abdominal wall
PublikacjaThe paper deals with a membrane model of a synthetic surgical mesh for treatment of abdominal hernia. The authors analyse the compatibility of two implant types with the human wall: Dualmesh Gore and Proceed. The finite element method is applied to simulate tjhe behavior of the proposed model. Due fact that recurrences are usually caused by connection failure, this study is focused on reaction forces in supports representing the...
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Recognition and sensing of anions
PublikacjaMolecular ion recognition is one of the most intensively studied areas of supramolecular technology. The reason for this is the essential role that ions play in many biological as well as industrial processes. On the other hand, however, it has been proved that ions can have a negative impact on human health and the environment. For these reasons, it is extremly important to develop rapid and simple methods allowing the determination...
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Modeling of active fiber Bragg grating underwater acoustic sensor.
PublikacjaW pracy przedstawiono wyniki modelowania nowego typu przetwornika hydroakustycznego wykorzystującego światłowodową siatkę Bragga. Siatka ta, domieszkowana jonami pierwiastków ziem rzadkich, pracuje jako laser z rozłożonym sprzężeniem zwrotnym, co pozwala na znaczne zwiększenie czułości tego prztwornika w porównaniu z rozwiązaniami wykorzystującymi siatki pasywne. W celu uzyskania równomiernego rozkładu naprężeń siatki zastosowano...
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High resolution X-ray diffractometry and reflectometry of semiconductor nano- and micro- structures based on X-ray refractive optics
PublikacjaIn this thesis proposed, discussed and studied novel synchrotron X-ray diffractometry and reflectometry methods based on a refractive optics. The experimental results obtained from the ID06 beamline at ESRF, Grenoble, France are presented and analyzed in this work to demonstrate a high angular and space resolution in addition to the opportunity to manage in situ and on operando experiments with the help of proposed X-ray optical...
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Badania właściwości nowego amperometrycznego czujnika ditlenku siarki.
PublikacjaZaprojektowany i wykonany został nowy, amperometryczny czujnik ditlenku siarki, wyposażony w elektrodę roboczą ze złota napylonego bezpośrednio na powierzchnię membrany oddzielającej komórkę elektrokatalityczną czujnika od otoczenia. Sygnał czujnika, związany z reakcją elektrodową utleniania ditlenku siarki, jest proporcjonalny do stężenia SO2. W tej pracy przedstawiono rezultaty badań czujnika z membranami z mikroporowatego Teflonu...
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Application of a cationic surfactant in the multichanel taste sensor
PublikacjaSubstancje należaće do czterech klas smaku były badane za pomocą pięciokanałowego sensora smaku składającego się z elektrod z membranami polimerowo-lipidowymi. Wykazanano, że elektroda zbudowana z membrany polimerowej zawierajacej kationowy surfaktant może byc z powodzeniem zastosoana w sensorze smaku do rozpoznania napojów składających sie głównie z sacharozy i kwasu cytrynowego produkowanych przez rózne firmy.
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A XAS study of a highly diluted Pt electrocatalyst operating in PEM fuel cells
PublikacjaWe present an X-ray absorption spectroscopy (XAS) study of a low Pt content catalyst layer(Pt loading 0.1 mg c2) operating at the cathode of a proton exchange membrane fuel cell(PEMFC). This catalyst is based on the use of a mesoporous inorganic matrix as a support forthe catalyst Pt nanoparticles. Due to the high Pt dilution, in situ measurements of itsstructural properties by XAS are challenging and suitable experimental strategies...
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Dynamics of mechanical model of implant-tissue system in ventral hernia repair
PublikacjaThe paper deals with a finite element modelling of implants in the problem of ventral hernia repair. The synthetic mesh implanted in the abdomen during surgery is here modelled as a membrane structure. Thesystem undergoes the internal abdominal pressure that occurs during the postoperative cough, the load identified in the literature as the main cause of the connection failure and hernia recurrence. The model can be used to estimate...
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Zminiaturyzowane czujniki potencjometryczne jako detektory do elektroforezy kapilarnej
PublikacjaWykorzystanie czujników potencjometrycznych jako detektorów w chromatografii cieczowej (LC) i elektroforezie kapilarnej (CE), będące interesującym rozwiązaniem nie jest praktycznie stosowane. Przeprowadzono elektroforezę kapilarną związków z grupy leków antyhistaminowych i antydepresantów stosując jako zminiaturyzowany czujnik potencjometryczny elektrodę metaliczną z polimerową membraną pozostającą w bezpośrednim kontakcie z powierzchnią...
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Modeling of active fiber Bragg grating sensors
PublikacjaW artykule omówiono działanie przetwornika hydrofonu wykorzystującego profilowaną membranę sprzężoną z aktywną światłowodową siatką Bragga. W wyniku modelowania, wykonanego Metodą Elementów Skończonych, stwierdzono występowanie gradientów naprężeń wzdłuż siatki. Opracowano program do modelowania pasywnych i aktywnych światłowodowych siatek Bragga wykorzystujący metodę modów sprzężonych. Przedstawiono charakterystyki widmowe pasywnych...
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Zastosowanie analizy wielokryterialnej do oceny i wyboru rozwiązania pokrycia dachowego
PublikacjaCelem artykułu jest wskazanie optymalnego rozwiązania materiałowego do wykonania pokrycia dachowego w budynku biurowym. Wykorzystując wywiady z ekspertami oraz badania ankietowe, dokonano oceny trzech wariantów materiałowych. W badaniu zastosowano analizę wielokryterialną MCE z uwzględnieniem jedenastu kryteriów oceny. Badania wskazały, iż najkorzystniejszym rozwiązaniem jest zastosowanie membrany EPDM. Pomimo największych nakładów...
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Trolejbusy z bateryjnym źródłem zasilania. Doświadczenia eksploatacyjne i koncepcja liniowego zastosowania w Gdyni
PublikacjaW opracowaniu przedstawiono doświadczenia zebrane podczas dwuletniej eksploatacji trolejbusów z pomocniczym źródłem zasilania w Gdyni. Zaprezentowano koncepcję zastosowania tych trolejbusów do obsługi osiedla Fikakowo