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Wyniki wyszukiwania dla: DRAD PROTEIN
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Kazimierz Darowicki prof. dr hab. inż.
OsobyStudia wyższe ukończyłem w czerwcu 1981 roku po zdaniu egzaminu dyplomowego i obronie pracy magisterskiej. Opiekunem pracy magisterskiej był dr hab. inż. Tadeusz Szauer. W roku 1991, 27 listopada uzyskałem stopień naukowy broniąc pracę doktorską zatytułowaną „Symulacyjna i korelacyjna analiza widm immitancyjnych inhibitowanej reakcji elektrodowej”. Promotorem pracy był prof. dr hab. inż. Józef Kubicki (Wydział Chemiczny...
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Meiothermus ruber cells as β-galactosidase activity biocatalyst
PublikacjaThe current study allowed to establish the possibility of using whole cells of thermophilic bacteria Meiothermus ruber as a biocatalyst with β-galactosidase activity. β-galactosidases are used for hydrolysis of lactose as well as for oligosaccharides synthesis. The advantages of using whole bacterial cells catalysis is not only elimination of tedious, expensive protein isolation and/or purification but also stabilization of enzymes...
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The possibility of use keratin products in various branches of industry
PublikacjaBird's feather, the main by-product of the poultry industry, are not enough utilized and negatively influencing the environment. They contain large amount of keratin, the protein with high cystine content. The aim of our work was to obtain hydrolysates and isolates of keratin, which can be used in various areas of food, cosmetics, pharmaceutical industry and as animal feed. Keratin can be hydrolyzed and extracted from feather after...
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Keep It Flexible: Driving Macromolecular Rotary Motions in Atomistic Simulations with GROMACS
PublikacjaWe describe a versatile method to enforce the rotation of subsets of atoms, e.g., a protein subunit, in molecular dynamics (MD) simulations. In particular, we introduce a “flexible axis” technique that allows realistic flexible adaptions of both the rotary subunit as well as the local rotation axis during the simulation. A variety of useful rotation potentials were implemented for the GROMACS 4.5 MD package. Application to the...
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Combined thrust radial bearing of a submarine main shaft – Design and analysis of failure
PublikacjaThis paper presents an analysis of the combined thrust radial bearing of a submarine propulsion shaft. The lubrication system of the bearing is based on a fixed ring. The efficiency of the lubrication system depends on the shaft speed and temperature, which affects oil viscosity. In turn, the thrust bearing load also depends on the rotational speed of the shaft, because as the speed increases, the drag of the ship increases simultaneously,...
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Recovery and Management of Postindustrial Spaces in the City on Selected Examples od European Implementations
PublikacjaUndeveloped, neglected and then abandoned post-industrial areas often located in a short distance from city centers have become, on one hand a major problem for city stewards due to the formation of dead urban tissue disturbing the continuity and cohesion of urbanized areas, on the other hand are spaces with high potential for a good location. The article aims to formulate, from the architectural and urban perspective, an answer...
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Ad hoc prayer spaces – a challenge of the architecture of the 21th century
PublikacjaThis article considers the problem of arranging places of prayer in the vicinity of universities and dormitories. A survey was conducted among students living in the dormitories of the Gdańsk University of Technology in order to determine if there was a real need to arrange such spaces. Subsequently, examples of temporary chapels designed in the last 20 years in Western European countries were presented. Analysis of the selected...
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Haemorrhage from respiratory passages, unspecified - Male, 71 - Tissue image [12030630017642631]
Dane BadawczeThis is the histopathological image of BRONCHUS AND LUNG tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Haemorrhage from respiratory passages, unspecified - Male, 71 - Tissue image [12030630017644711]
Dane BadawczeThis is the histopathological image of BRONCHUS AND LUNG tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Haemorrhage from respiratory passages, unspecified - Male, 71 - Tissue image [12030630017645711]
Dane BadawczeThis is the histopathological image of BRONCHUS AND LUNG tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Haemorrhage from respiratory passages, unspecified - Male, 71 - Tissue image [12030630017648421]
Dane BadawczeThis is the histopathological image of BRONCHUS AND LUNG tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Haemorrhage from respiratory passages, unspecified - Male, 71 - Tissue image [12030630017647721]
Dane BadawczeThis is the histopathological image of BRONCHUS AND LUNG tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Beneficial effects and potential risks of tomato consumption for human health: An overview
PublikacjaTomato and its derived products have a very interesting nutritional value in addition to prominent antioxidant,anti-inflammatory, and anticancer activities. In terms of tomatoes are generally quite safe to eat. However,overall consumption varies from individual to individual. Indeed, either beneficial or harmful effects of plantsor their derived products are closely related to quality, including the presence of biologically active...
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IFI16 Impacts Metabolic Reprogramming during Human Cytomegalovirus Infection
PublikacjaCellular lipid metabolism plays a pivotal role in human cytomegalovirus (HCMV) infection, as increased lipogenesis in HCMV-infected cells favors the envelopment of newly synthesized viral particles. As all cells are equipped with restriction factors (RFs) able to exert a protective effect against invading pathogens, we asked whether a similar defense mechanism would also be in place to preserve the metabolic compartment from HCMV...
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Nutrients and Bioactive Components of Human Milk After one Year of Lactation. Implication for Human Milk Banks
PublikacjaObjective: Specifying the nutrient content and bioactive compounds in milk from long-term lactation for the purpose of finding additional sources of donors’ milk for HMBs. Method: Human milk samples were collected from 43 mothers of term infants (term infant human milk, TIHM) (3–6 weeks of lactation) and 50 mothers who have breastfed for over a year (long-nursing human milk, LNHM). The milk collection time was 24 hours. The analyses...
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A new B-D-galactosidase with a low temperature optimum isolated from the Antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization.
PublikacjaA psychrotrophic bacterium producing a coldadaptedB-galactosidase upon growth at low temperatureswas classiWed as Arthrobacter sp. 20B. A genomic DNAlibrary of strain 20B introduced into Escherichia coliTOP10F' and screening on X-Gal (5-bromo-4-chloro-3-indolyl-B-D-galactopyranoside)-containing agar plates ledto the isolation of B-galactosidase gene. The B-galactosidasegene (bgaS) encoding a protein of 1,053 amino acids,with a...
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Molecular mechanism and energetics of coupling between substrate binding and product release in the F 1 -ATPase catalytic cycle
PublikacjaF1-ATPase is a motor protein that couples the rotation of its rotary γ subunit with ATP synthesis or hydrolysis. Single-molecule experiments indicate that nucleotide binding and release events occur almost simultaneously during the synthesis cycle, allowing the energy gain due to spontaneous binding of ADP to one catalytic β subunit to be directly harnessed for driving the release of ATP from another rather than being dissipated...
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Nonlinear secondary arc model use for evaluation of single pole auto-reclosing effectiveness
PublikacjaPurpose – The purpose of this paper is to discuss two evaluation methods of single pole autoreclosing process effectiveness in HV transmission lines. Secondary arc current and recovery voltage results obtained by load flow calculation are compared to the results obtained by the time domain simulations. Moreover, a nonlinear secondary arc implementation is presented. Design/methodology/approach – A computer simulation studies were...
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Manufacturing and Recycling Impact on Environmental Life Cycle Assessment of Innovative Wind Power Plant Part 1/2
PublikacjaWind power plants are considered as ecologically-clean source of energy. However, manufacturing processes cannot be treated that way. Manufacturing processes consume huge amount of electrical and thermal energy and significant amount of materials, e.g. steel, polymers, oils and lubricants. All of the above could be potentially harmful for environment. There are not many works and publications regarding life-cycle analysis of wind...
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Determinants of Directionality and Efficiency of the ATP Synthase Fo Motor at Atomic Resolution
PublikacjaFo subcomplex of ATP synthase is a membrane-embedded rotary motor that converts proton motive force into mechanical energy. Despite a rapid increase in the number of high-resolution structures, the mechanism of tight coupling between proton transport and motion of the rotary c-ring remains elusive. Here, using extensive all-atom free energy simulations, we show how the motor’s directionality naturally arises from the interplay...
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Modelling of bio-mechanical objects: recent examples
PublikacjaW opracowaniu przedstawiono dwa wykorzystania modelowania bio-mechanicznego wybranych obiektów: modelowanie zachowania mięśni w układzie ramię-przedramię oraz wstępny projekt protezy dłoni przy użyciu materiałów z pamięcią kształtu. Przedstawiono wyniki badań symulacyjnych oraz opis dynamiki działania układów sterowania dla protezy dłoni oraz dla układu ramię-przedramię.
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Parity vertex colouring of graphs
PublikacjaA parity path in a vertex colouring of a graph is a path along which each colour is used an even number of times. Let Xp(G) be the least number of colours in a proper vertex colouring of G having no parity path. It is proved that for any graph G we have the following tight bounds X(G) <= Xp(G) <=|V(G)|− a(G)+1, where X(G) and a(G) are the chromatic number and the independence number of G, respectively. The bounds are improved for...
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Immobilized biocatalysts - useful tool for industrial processes
PublikacjaThe main goal of biomaterials immobilization is the industrial re-use of them for many reaction cycles. However, application of this technolgoy for full commercialization requires more experiments in this field. In this study thermostable beta-galactosidases from Ptrococcus woesei and Meiothermus ruber were taken as model proteins immobilized in alginate gel.
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Waldenstrom macroglobulinemia - Female, 50 - Tissue image [3300730069459481]
Dane BadawczeThis is the histopathological image of HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Waldenstrom macroglobulinemia - Female, 50 - Tissue image [3300730069453581]
Dane BadawczeThis is the histopathological image of HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Waldenstrom macroglobulinemia - Female, 50 - Tissue image [11290630017293601]
Dane BadawczeThis is the histopathological image of HEMATOPOIETIC AND RETICULOENDOTHELIAL SYSTEMS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Instytut Ekspertyz Sądowych im. Prof. dra Jana Sehna w Krakowie
Wydawnictwa -
Lucyna Nyka prof. dr hab. inż. arch.
OsobyLucyna Nyka (prof. dr hab. inż. arch.) jest architektem i profesorem na Wydziale Architektury Politechniki Gdańskiej. W latach 2008-2016 pełniła funkcję prodziekana ds. nauki, a od 2016 jest dziekanem Wydziału Architektury. Zainteresowania badawcze prof. Lucyny Nyki skoncentrowane są wokół kwestii powiązań architektury i wody, przekształceń terenów nadwodnych oraz urbanistycznych krajobrazów. Jest autorem i współautorem wielu...
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Simplified AutoDock force field for hydrated binding sites
Publikacjahas been extracted from the Protein Data Bank and used to test and recalibrate AutoDock force field. Since for some binding sites water molecules are crucial for bridging the receptor-ligand interactions, they have to be included in the analysis. To simplify the process of incorporating water molecules into the binding sites and make it less ambiguous, new simple water model was created. After recalibration of the force field on...
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Joanna Kabrońska dr inż. arch.
OsobyDoktorat z wyróżnieniem: Forma rozwiązań architektonicznych jako droga realizacji idei biblioteki przyszłości, 1994 Laureatka IV Międzynarodowego Biennale Architektury w Krakowie, 1991 Stypendystka DAAD post-doctoral scholarship, Berlin, 2002 Autorka publikacji dotyczących relacji architektury, sztuki i pamięci, między innymi monografii Architektura jako forma pamięci. Rola architektury w tworzeniu współczesnego horyzontu wartości,...
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Computing internodal conductivities in numerical modeling of two dimensional unsaturated flow on rectangular grid
PublikacjaW artykule porównano rozwiązania numeryczne dwuwymiarowego równania przepływu nienasyconego z użyciem różnych metod uśredniania współczynnika przewodności hydraulicznej między sąsiednimiwęzłami. Uwzględniono średnia arytmetyczną, geometryczną, "pod prad" oraz całkową, a także niedawno zaproponowany sposób uśredniania oparty na analizie przepływu ustalonego. Ten ostatni sposób okazałsię najbardziej uniwersalny, gdyż umożliwiał otrzymanie...
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Study of the influence of medium composition on motility and aggregation of recombinant Escherichia coli strain AAEC191A/pCC90 Dra D-mut-M9 + 0.5% cassaminic acids, M9 + 0.5% cassaminic acids + 0.2% glucose, M9 + 0.5% cassaminic acids + 0.5% glucose
Dane BadawczeMicrobial motility is a fundamental aspect of many microbial life cycles and is a key survival mechanism that enables microorganisms to navigate diverse and dynamic environmental conditions. This phenomenon becomes particularly important in response to changes in stimuli in time and space. The following experiment aimed to investigate how the composition...
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New recombinant chimeric antigens, P35-MAG1, MIC1-ROP1, and MAG1-ROP1, for the serodiagnosis of human toxoplasmosis
PublikacjaThe aim of the study was to evaluate the usefulness of 3 chimeric Toxoplasma gondii antigens, P35-MAG1,MIC1-ROP1 andMAG1-ROP1, in the serodiagnosis of an acute toxoplasmosis in humans. Proteins were produced as fusion proteins containing His tags ends and then further purified by metal affinity chromatography. Their application for the diagnosis of recently acquired T. gondii infection was tested in IgG and IgM enzyme-linked immunosorbent...
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Synthesis, characterization and application of a zirconium-based MOF-808 functionalized with isonicotinic acid for fast and efficient solid phase extraction of uranium(VI) from wastewater prior to its spectrophotometric determination
Publikacja -
Total restrained bondage in graphs
PublikacjaPodzbiór D zbioru wierzchołków grafu nazywamy zewnętrznie totalnym dominującym w grafie, jeśli każdy wierzchołek spoza D ma sąsiada zarówno w D jak i poza D. Moc najmniejszego zbioru o tej własności nazywamy liczbą dominowania zewnętrznie totalnego. W artykule badamy wpływ usuwania krawędzi na liczbę dominowania zewnętrznie totalnego, czyli liczbę zewnętrznego totalnego zniewolenie w grafach.
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Detection of cellulose production capacity of recombinant Escherichia coli strains BL21(DE3) and AAEC191A
Dane BadawczeBacteria that form biofilms generate an extracellular matrix (ECM), where cellulose stands out as a key constituent. An approach for assessing microorganisms' cellulose production involves using calcofluor white staining on colonies. In this method, a fluorescent dye (calcofluor-white) is introduced to a stable YESCA substrate composed of casamino acids,...
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Detection of cellulose production capacity of recombinant Escherichia coli strains BL21(DE3) and AAEC191A
Dane BadawczeBacteria that form biofilms generate an extracellular matrix (ECM), where cellulose stands out as a key constituent. An approach for assessing microorganisms' cellulose production involves using calcofluor white staining on colonies. In this method, a fluorescent dye (calcofluor-white) is introduced to a stable YESCA substrate composed of casamino acids,...
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Detection of cellulose production capacity of recombinant Escherichia coli strains BL21(DE3) and AAEC191A
Dane BadawczeBacteria that form biofilms generate an extracellular matrix (ECM), where cellulose stands out as a key constituent. An approach for assessing microorganisms' cellulose production involves using calcofluor white staining on colonies. In this method, a fluorescent dye (calcofluor-white) is introduced to a stable YESCA substrate composed of casamino acids,...
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Detection of cellulose production capacity of recombinant Escherichia coli strains BL21(DE3) and AAEC191A
Dane BadawczeBacteria that form biofilms generate an extracellular matrix (ECM), where cellulose stands out as a key constituent. An approach for assessing microorganisms' cellulose production involves using calcofluor white staining on colonies. In this method, a fluorescent dye (calcofluor-white) is introduced to a stable YESCA substrate composed of casamino acids,...
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Macromodels for Efficient Analysis of Open-Region Problems Using the Finite Element Method
PublikacjaThis paper presents a local model-order reduction, called macromodeling, applied to speed-up the simulations of open-region problems, analyzed by means of finite element method. This technique is illustrated by a numerical example, which deals with a dielectric resonator antenna (DRA). The obtained results show that the proposed approach is reliable and can significantly increase the standard finite element method efficiency.
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Applying of Doped Graphene Oxide Coatings for Corrosion Prevention
PublikacjaINTRODUCTION Graphene is a carbonaceous material characterized by extraordinary properties (high electron mobility, high surface area, high mechanical strength of 1100 GPa, very dense network hindering the passage of even the smallest helium atoms) [1]. Therefore, it found many applications, also as an anti-corrosive layer [2]. Electrophoretic Deposition (EPD) is one of the methods to deposit coatings. However, due to slight solubility...
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The usefulness of Toxoplasma gondii MIC1-MAG1-SAG1 chimeric antigen in the serodiagnosis of ovine toxoplasmosis
PublikacjaToxoplasmosis is a zoonotic disease caused by the protozoan Toxoplasma gondii, which infects humans and most warm-blooded animals throughout the world. Although human toxoplasmosis in healthy adults is usually asymptomatic, a serious disease can occur in the case of congenital infection and immunocompromised individuals. Among food animals, sheep, along with goats and pigs, possess the highest incidence of T. gondii cysts in meat,...
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Membrane separation processes for the extraction and purification of steviol glycosides: an overview
PublikacjaSteviol glycosides (SGs), as natural sweeteners from Stevia rebaudiana, are currently employed for replacing sugar and its derivatives in several food products and formulations. Such compounds play an essential role in human health. Their usage provides a positive effect on preventing diseases related to sugar consumption, including diabetes mellitus, cancer, and lipid metabolism disorders. The traditional extraction of SGs is...
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Functional properties of edible films for food applications - a review
PublikacjaApplication of natural polymers to forming edible films for food applications has been reviewed. Due to the hydrophilic nature of proteins and polysaccharides, films prepared from these polymers are too susceptible to moisture. Modification of them with critically selected a suitable compounds can result in their improved functional properties and making them useful for food packaging.
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Adam Inglot dr inż.
OsobyAdam Inglot (urodzony 3 października 1984 w Rzeszowie) studiował Inżynierię Środowiska oraz Geodezję i Kartografię na Wydziale Geodezji Górniczej i Inżynierii Środowiska Akademii Górniczo-Hutniczej w Krakowie. Inżynierię Środowiska ukończył w 2011 w stopniu mgr inż. broniąc pracę „Weryfikacja przydatności modelu GeoMod do prognozowania procesu urbanizacji w aglomeracji krakowskiej” u dra inż. Wojciecha Drzewieckiego. Natomiast...
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New PCR test for detection of Candida glabrata based on the molecular target chosen by the RAPD technique
PublikacjaRapid, reliable diagnosis is a necessary condition for the successful treatment of infections. Such diagnostic assays are continually being developed. #e paper presents a method for selecting the molecular target for PCR-based diagnostics based on the comparison of RAPD patterns. A sequence encoding Candida glabrata CBS138 hypothetical protein was selected. The limit of detection for PCR and real-time PCR reactions with DNA extracted...
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Subsoil degradation effect in reliability analysis of the jack-up platform structure
PublikacjaStructural reliability analysis is considered, by FORM and SORM applied to a certain idealized soil-platform structure interaction problem due to cyclic water wave and wind loads. Wave and wind loads are random variables, whereas dead load is deterministic. Load parameters are typical for storm conditions in the Baltic Sea. The soil-structure interaction is idealized as a set of linear translational and rotational springs. Due...
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Addressing the Weaknesses of Multi-Criteria Decision-Making Methods using Python
PublikacjaThe book aims to draw attention to the weaknesses in Multi-Criteria Decision-Making (MCDM) methods and provide insights to improve the decision-making process. By addressing these weaknesses, it seeks to enhance the accuracy and effectiveness of MCDM methods in selecting the best alternatives in various fields. The book covers popular MCDM methods such as TOPSIS, ELECTRE, VIKOR, and PROMETHEE. It compares traditional methods with...
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Study of the influence of medium composition on motility and aggregation of recombinant Escherichia coli strain BL21(DE3)/pCC90 Dra D-mut-M9 + 0.5% cassaminic acids, M9 + 0.5% cassaminic acids + 0.2% glucose, M9 + 0.5% cassaminic acids + 0.5% glucose
Dane BadawczeMicrobial motility is a fundamental aspect of many microbial life cycles and is a key survival mechanism that enables microorganisms to navigate diverse and dynamic environmental conditions. This phenomenon becomes particularly important in response to changes in stimuli in time and space. The following experiment aimed to investigate how the composition...
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CYP3A4 overexpression enhances apoptosis induced by anticancer agent imidazoacridinone C-1311, but does not change the metabolism of C-1311 in CHO cells
PublikacjaWe examine whether CYP3A4 overexpression influences the rate and pattern of antitumor imidazoacridinone C-1311 metabolism, in relation to the impact of this overexpression on cell cycle progression and final cellular response of CHO cells following C-1311 treatment. Methods: Three CHO cell lines: CHO-WT, wild type, CHO-HR, overexpressing cytochrome P450 reductase (CPR) and CHO-HR-3A4, coexpressing CPR and CYP3A4 were applied....