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The influence of Nigella sativa essential oil on proliferation, activation, and apoptosis of human T lymphocytes in vitro
PublikacjaIn previous work, we tested the immunomodulatory effect of Nigella sativa (NS) fatty oil. Our results demonstrated that unrefined, obtained by cold pressing black cumin seed oil inhibited lymphocytes’ proliferation and induced their apoptosis in a dose-dependent manner. In this study, we examined the immunomodulatory properties of essential oil (EO) obtained from the NS seeds by hydrodistillation and its two main constituents:...
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Which casein in sodium caseinate is most resistant to in vitro digestion? Effect of emulsification and enzymatic structuring
PublikacjaWe investigated the resistance of individual constituent casein epitopes (αS1-, αS2-, β- and κ-CN) in food-grademilk protein sodium caseinate (NaCN) to simulated human gastro-duodenal digestion. The influence of NaCNadsorption to the surface of oil-in-water emulsion droplets and the effect of crosslinking of the protein withenzyme transglutaminase (TG) on the proteolysis were studied by indirect ELISA. TG crosslinking renderedfragments...
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Static in vitro digestion model adapted to the general older adult population: an INFOGEST international consensus
PublikacjaUnderstanding the mechanisms of food digestion is of paramount importance to determine the effect foods have on human health. Significant knowledge on the fate of food during digestion has been generated in healthy adults due to the development of physiologically-relevant in vitro digestion models. However, it appears that the performance of the oro-gastrointestinal tract is affected by ageing and that a model simulating the digestive...
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Results of pharmaceuticals mixtures toxicity on inhibition of bioluminescence of Vibrio fischeri (Microtox)
Dane BadawczeThe research was concerned with verifying the impact of mixtures of nine pharmaceuticals against a selected organism, i.e., the bacterium Aliivibrio fischeri. A. fisheri is used as a model organism in the monitoring of acute toxicity in environmental and reference samples in Microtox® systems. Tested pharmaceuticals, namely: diclofenac (sodium salt),...
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A new hybrid method for analysis of scattering from arbitrary configuration of cylindrical objects
PublikacjaArtykuł opisuje nową hybrydową metodę analizy rozpraszania fali elektromagnetycznej od obiektów cylindrycznych o dowolnym przekroju poprzecznym. W prezentowanym podejściu każdy obiekt jest traktowany jako efektywny cylinder o przekroju kołowym, opisany przez macierz impedancyjną. W celu uzyskania owej macierzy zastosowano połączenie metod różnic skończonych w dziedzinie częstotliwości (FDFD) i dopasowania rodzajów (MM). W celu...
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Influence of instalation of piles with partial and full displacement of the soil on the subsoil strength
PublikacjaZjawiska zachodzące w bezpośrednim sąsiedztwie pobocznicy pali przemieszczeniowych mają ważne znaczenie dla ich pracy w podłożu. Zmiany stanu naprężenia pionowego, poziomego, zmiana struktury gruntu, wilgotności i zagęszczenia stwarza nowe warunki oddziaływania w strefie pobocznica - podłoże. Przedstawiono wyniki badań sondą CPT w sąsiedztwie pali CFA dla gruntów uwarstwionych, niespoiste / spoiste. Zwrócono uwagę na dobór parametrów...
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''Przewód zielono-żółty'' ale ''wyłącznik różnicowoprądowy''. Pisownia przymiotników złożonych: z łącznikiem czy łączna?
PublikacjaNotatka wyjaśniająca zasady pisowni przymiotników złożonych: pisownia z łącznikiem czy łączna.
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FE-investigations of granular flow in silos using an uncoupled ALE-formulation and a non-local hypoplastic model
PublikacjaArtykuł omawia wyniki naporu materiału sypkiego podczas przepływu w silosach z i bez wkładek. Obliczenia wykonano wykorzystując niepołączone sformułowanie ALE na bazie prawa sprężysto-plastycznego według Druckera-Pragera. Zbadano wpływ kształtu i położenia wkładki na wielkość naporu i rodzaj przepływu.
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The Polish Version of the Yale-Brown Obsessive-Compulsive Scale, Modified to Reflect Obsessions and Compulsions Related to Heavy Drinking (YBOCS-hd-PL)
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Phenylalanine derivatives with modulating effects on human α1-glycine receptors and anticonvulsant activity in strychnine-induced seizure model in male adult rats
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Disorder of Biological Quality and Autophagy Process in Bovine Oocytes Exposed to Heat Stress and the Effectiveness of In Vitro Fertilization
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In Vivo, In Vitro and In Silico Studies of the Hybrid Compound AA3266, an Opioid Agonist/NK1R Antagonist with Selective Cytotoxicity
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Flavonoids as tyrosinase inhibitors in in silico and in vitro models: basic framework of SAR using a statistical modelling approach
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In Vitro Anticancer Potential of Jasione montana and Its Main Components against Human Amelanotic Melanoma Cells
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Effect of nanosilver (nAg) on disinfection, growth, and chemical composition of young barley leaves under in vitro conditions
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In vitro antioxidant and anti-inflammatory activities of extracts from Potentilla recta and its main ellagitannin, agrimoniin
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Inulin and fructooligosaccharide affect in vitro calcium uptake and absorption from calcium-enriched gluten-free bread
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Adenoviral Transduction of Mesenchymal Stem Cells: In Vitro Responses and In Vivo Immune Responses after Cell Transplantation
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In Vitro and In Vivo Imaging-Based Evaluation of Doxorubicin Anticancer Treatment in Combination with the Herbal Medicine Black Cohosh
PublikacjaAs a substitution for hormone replacement therapy, many breast cancer patients use black cohosh (BC) extracts in combination with doxorubicin (DOX)-based chemotherapy. In this study, we evaluated the viability and survival of BC- and DOX-treated MCF-7 cells. A preclinical model of MCF-7 xenografts was used to determine the influence of BC and DOX administration on tumor growth and metabolism. The number of apoptotic cells after...
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The bile salt/phospholipid ratio determines the extent of in vitro intestinal lipolysis of triglycerides: Interfacial and emulsion studies
PublikacjaThis study focused on the protein-stabilised triglyceride (TG)/water interfaces and oil-in-water emulsions, and explored the influence of varying molar ratios of bile salts (BSs) and phospholipids (PLs) on the intestinal lipolysis of TGs. The presence of these two major groups of biosurfactants delivered with human bile to the physiological environment of intestinal digestion was replicated in our experiments by using mixtures...
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In Vitro Studies on Nanoporous, Nanotubular and Nanosponge-Like Titania Coatings, with the Use of Adipose-Derived Stem Cells
PublikacjaIn vitro biological research on a group of amorphous titania coatings of different nanoarchitectures (nanoporous, nanotubular, and nanosponge-like) produced on the surface of Ti6Al4V alloy samples have been carried out, aimed at assessing their ability to interact with adipose-derived mesenchymal stem cells (ADSCs) and affect their activity. The attention has been drawn to the influence of surface coating architecture and its physicochemical...
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[18F]Amylovis as a Potential PET Probe for β-Amyloid Plaque: Synthesis, In Silico, In vitro and In vivo Evaluations
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Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro
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An In Vitro Anticancer, Antioxidant, and Phytochemical Study on Water Extract of Kalanchoe daigremontiana Raym.-Hamet and H. Perrier
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In vitro and in vivo evaluation of sandwich-like mesoporous silica nanoflakes as promising anticancer drug delivery system
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Potential Use of Silica Nanoparticles for the Microbial Stabilisation of Wine: An In Vitro Study Using Oenococcus oeni as a Model
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In vitro biodegradation of bacterial nanocellulose under conditions simulating human plasma in the presence of selected pathogenic microorganisms
PublikacjaThe Biodegradability of bacterial nanocellulose (BNC) was assessed based on a change in its properties under conditions simulating human plasma in the presence or absence of the pathogens Staphylococcus aureus, Candida albicans and Aspergillus fumigatus. It was shown that the dry mass of BNC did not change during 6 months of incubation, except for samples stored in the presence of moulds, where the dry mass reduced by 40 %. The...
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Zinc Oxide and Zinc Oxide Nanoparticles Impact on In Vitro Germination and Seedling Growth in Allium cepa L.
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Expression pattern of new genes regulating female sex differentiation and in vitro maturational status of oocytes in pigs
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Protein oligomerization is the biochemical process highly up-regulated in porcine oocytes before in vitro maturation (IVM)
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In vitro substance P-dependent induction of bone marrow cells in common (CD10) acute lymphoblastic leukaemia
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Chocolate desserts with ricotta hydrolysates: In vitro study of inhibitory activity against angiotensin‐converting enzyme and cholinesterase
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Chicken feather keratin as a source of bioactive peptides useful in prevention of metabolic disorders – in silico and in vitro hydrolysis
PublikacjaProtein derived bioactive peptides not only serve as nutrients but can also exert drug-like activity, e.g. antidiabetic, antihypertensive, or antimicrobial, to name a few. Most biopeptides have beneficial effects on health that make them attractive for nutraceutical applications. The majority of bioactive peptides have been obtained from food proteins, but protein-rich by-products generated by agriculture...
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Application of bacterial cellulose experimental dressings saturated with gentamycin for management of bone biofilm in vitro and ex vivo
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In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens
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The processing of staphylococcal biofilm images from Fluorescence and Confocal Microscopy to assess in vitro efficacy of antiseptic molecules
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A Potential Relationship Between Estrogen Receptors Polymorphisms, Sperm Function and in vitro Fertilization Success: A Preliminary Study*
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GC-MS analysis, antidiabetic and antioxidant activity of methanolic extract of pluteus cervinus: an in vitro and in silico approach
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The thermostability, bioactive compounds and antioxidant activity of some vegetables subjected to different durations of boiling: investigation in vitro
PublikacjaThis research was performed in order to compare the water and acetone extracts of raw and boiled for 10, 20, 40 and 60 min Korean lotus roots (KLR) and Polish white onion (PWO) in the contents of theirbioactive compounds, antioxidant activity and thermostability.It was found that polyphenols (mg GAE/g), flavanols (mg GAE/g), flavonoids (mg CE/g), anthocyanins (mg CGE/kg) and tannins (mg CE/g) in water extract of raw lotus roots...
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The termostability, bioactive compounds and antioxidant activity of some vegetables subjected to different durations of boiling: investigation in vitro.
PublikacjaThis research was performed in order to compare the water and acetone extracts of raw and boiled for 10, 20, 40 and 60 min Korean lotus roots (KLR) and Polish white onion (PWO) in the contents of theirbioactive compounds, antioxidant activity and thermostability. It was found that polyphenols (mg GAE/g), flavanols (mg GAE/g), flavonoids (mg CE/g), anthocyanins(mg CGE/kg) and tannins (mg CE/g) in water extract of raw lotus roots...
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In Vitro Studies on the Relationship Between the Antioxidant Activities of Some Berry Extracts and Their Binding Properties to Serum Albumin
PublikacjaThe aim of this study was to investigate the possibility to use the bioactive components from cape gooseberry (Physalis peruviana), blueberry (Vaccinium corymbosum), and cranberry (Vaccinium macrocarpon) extracts as a novel source against oxidation in food supplementation. The quantitative analysis of bioactive compounds (polyphenols, flavonoids, flavanols, carotenoids, and chlorophyll) was based on radical scavenging spectrophometric assays...
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Isolation and structure elucidation of phenolic compounds from Cyclopiasubternata Vogel (honeybush) intact plant and in vitro cultures
PublikacjaZ materiału roślinnego i hodowli tkankowych Cyclopia subternata Vogel (miodokrzew) wyizolowano i zidentyfikowano matodami NMR i MS 10 związków. m.in. z grupy flawonów i glikozydów izoflawonów. 6 z nich po raz pierwszy wykryto w tym gatunku roślin.
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The Distribution of Glucosinolates in Different Phenotypes of Lepidium peruvianum and Their Role as Acetyl- and Butyrylcholinesterase Inhibitors—In Silico and In Vitro Studies
PublikacjaThe aim of the study was to present the fingerprint of different Lepidium peruvianum tu- ber extracts showing glucosinolates-containing substances possibly playing an important role in preventinting dementia and other memory disorders. Different phenotypes of Lepidium peruvianum (Brassicaceae) tubers were analysed for their glucosinolate profile using a liquid chromatograph coupled with mass spectrometer (HPLC-ESI-QTOF-MS/MS platform)....
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INFOGEST inter-laboratory recommendations for assaying gastric and pancreatic lipases activities prior to in vitro digestion studies
PublikacjaIn vitro digestion studies often use animal digestive enzyme extracts as substitutes of human gastric and pancreatic secretions. Pancreatin from porcine origin is thus commonly used to provide relevant pancreatic enzymes such as proteases, amylase and lipase. Rabbit gastric extracts (RGE) have been recently introduced to provide gastric lipase in addition to pepsin. Before preparing simulated gastric and pancreatic extracts with...
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The assessment of load-settlement curve for Atlas piles correlated with CPT tests
PublikacjaRacjonalne stosowanie pali przemieszczeniowych typu Atlas wymaga analitycznej oceny pełnej krzywej obciążenie - osiadanie. W pracy przedstawiono możliwości wykorzystania metody funkcji transformacyjnych t-z (dla pobocznicy) oraz q-z (dla podstawy) dla oceny krzywej osiadania. Jednostkowe graniczne opory na pobocznicy, tmax, oraz pod podstawą, gf, określono na podstawie metody bezpośredniej, wykorzystując opory stożka sondy, qc....
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The influence of compensate coils on own ship magnetic field
PublikacjaA ferromagnetic object placed in the earth magnetic field causes a local disturbance in this field distribution. The field distribution disturbance is a function of many arguments like the dimensoins and shape of the object, its ferromagnetic properties ande its positon in relation to the earth magnetic field. in order to minimize the influence of this disturbance one places compensate coils on the ship. Some chosen results of...
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The effect of pH change of oxyteracycline solutions on the on inhibition of bioluminescence of Vibrio fischeri (Microtox)
Dane BadawczeIn this study attempt was also made to determine whether a pH change of the sample containing oxyteracycline solutions affects the total toxicity of the sample using the Microtox®
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Determination of the effective concentration of oxyteracycline solutions on the inhibition of bioluminescence of Vibrio fischeri (Microtox)
Dane BadawczeIn this study an attempt was made to determine the effective concentration for oxytetracycline solutions on the inhibition of bioluminescence of Vibrio fischeri (Microtox).
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The loss of essential oil components induced by the Purge Time in the Pressurized Liquid Extraction (PLE) procedure of Cupressus sempervirens
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FE investigations of dynamic shear localization in granular bodies within non-local hypoplasticity using ALE formulation
PublikacjaW artykule pokazano wyniki MES dotyczące powstawania lokalizacji odkształceń stycznych w materiałach granulowanych w 2 problemach dynamicznych: podczas ściskania dwuosiowego oraz przepływu silosowego. Obliczenia wykonano na bazie nielokalnego prawa hipoplastycznego. Zastosowano podejście mieszane lagranżowsko-eulerowskie.