Search results for: PROTEINASE INHIBITORS

Search results for: PROTEINASE INHIBITORS

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Minimum inhibitory concentrations (MICs) determination of selected human topoisomerase II alpha and bacterial DNA gyrase inhibitors against fungal strains
Open Research Data
open access
The datasets contain the results of determining the MIC (Minimal Inhibitory Concentration) value of known compounds* (inhibitors of human topoisomerase II alpha and bacterial DNA gyrase) against C. albicans SC5314, C. glabrata ATCC 90030, C. krusei ATCC 6258 and C. parapsilosis ATCC 22019 and Saccharomyces cerevisiae ATCC 9763 by the modified M27-A3...
Antifungal activity of L-homoserine O-acetyltransferase (CaMet2p) inhibitors.
Open Research Data
open access
The dataset contains a set of optical densities at 600 nm measured at microplate reader to determine the rate of fungal growth in the presence of Candida albicans homoserine O-acetyltransferase inhibitors. Antifungal activities were determined against several strains from Candida spp. and others by the modified M27-A3 method specified by the CLSI. We...
The electrochemical studies of CB-PLA electrode after surface activation by proteinase K digestion
Open Research Data
open access
- J. Ryl
- A. Koterwa
- P. Niedziałkowski
- M. Cieślik
- I. Kaczmarzyk
- S. Mania
- R. Tylingo
- series: Additive manufacturing technology of electroactive three-dimensional structures from diamond reinforced polylactide composites
The dataset contains the electrochemical studies performed to evaluate surface activation of the CB-PLA 3D printed electrodes by enzymatic hydrolysis in a solution containing proteinase K (72h digestion period). Different enzyme concentrations were evaluated: 0.2, 0.4, 0.6 and 0.8 mg/ml. The studies were performed before surface activation, during and...
Generation of inhibitory compounds during pre-treatment of lignocellulosic biomass
Open Research Data
open access
- K. Kucharska
- Z. Honarmandrad
Dataset contains calibration curves and typical resultsa obtained during biomass pre-treatment. Data are converted from digital raw files to xlsx files
SEM micrographs of aluminium corrosion in alkaline media with various bee products as corrosion inhibitors
Open Research Data
open access
- J. Jasonek
- J. Ryl
This dataset contains various scanning electron microscopy (SEM) micrographs of AA5754 aluminum alloy samples exposed to the corrosive alkaline environment without and with the addition of various bee products to act as green corrosion inhibitors. The products are bee pollen, honey, and putty. They were investigated in bicarbonate buffer (pH =10 or...
Effect of antitumor compounds on the yeast topoisomerase II relaxation activity
Open Research Data
open access
The datasets contain results of antitumor compounds* (known inhibitors of human topoisomerase alpha II) inhibitory activity against yeast topoisomerase II. DNA topoisomerases (Topo) are enzymes that catalyze changes in the spatial structure of DNA and play an important role in replication, transcription and recombination. Beyond their normal functions,...
The mechanism of internalization study of QDgreen−CD−FA−C−2028 conjugate at IC80 value to cancer (H460, Du-145, and LNCaP) and normal (MRC-5 and PNT1A) cells
Open Research Data
open access
- J. Pilch
The influence of different endocytosis inhibitors on the internalization of QDgreen−CD−FA−C−2028 conjugate at IC80 value in cancer (H460, Du-145, and LNCaP) and normal (MRC-5 and PNT1A) cells. First, the cells were preincubated with: drug-free medium (no inhibitor), at 4 °C, 5 µM Cytochalasin D, 30 µM Amiloride, 80 µM Dynasore, 25 µM Pitstop 2 and 1.5...
The electrochemical studies of CB-PLA electrode after surface activation by proteinase K digestion with different times and concentrations
Open Research Data
open access
- J. Ryl
- A. Koterwa
- P. Niedziałkowski
- M. Cieślik
- I. Kaczmarzyk
- S. Mania
- R. Tylingo
- series: Additive manufacturing technology of electroactive three-dimensional structures from diamond reinforced polylactide composites
The dataset contains the electrochemical studies performed to evaluate surface activation of the CB-PLA 3D printed electrodes by enzymatic hydrolysis in a solution containing proteinase K. DIfferent proteinase K concentrations and digestion times were evaluated, as described in subfolder names. The studies were performed before surface activation, during...
Determination of denaturation temperature of connective tissue proteins by viscometric measurements.
Open Research Data
open access
- R. Tylingo
- S. Mania
Viscometric denaturation temperature of connective tissue proteins was determined by measuring viscosity with a Brookfield viscometer, using a LV SC4 - 18, 25 or LV3 spindle and shear rates from 50 to 110 s-1 (Fig.1). Freeze-dried protein samples were dissolved in 0.5 M acetic acid at 4 °C and concentration of 20 mg/mL. The solution was stirred with...
The corrosion studies of 3,4,5-trihydroxybenzoic acid as an effective corrosion inhibitor of low alloy steel
Open Research Data
open access
- A. Rudol
- J. Ryl
The dataset contains the electrochemical studies evaluating if gallic acid is a corrosion inhibitor for low alloy steel. Three measurements were carried out each case; corrosion potential (label ecorr), electrochemical impedance spectroscopy (label eis) and cyclic polarization (label cp). The measurements were carried out in sodium chloride, acidified...
Determination of the minimum inhibitory concentration of C-1305 derivatives (IKE1-IKE8) against Candida strains
Open Research Data
open access
- I. Gabriel
- E. Paluszkiewicz
- K. Kozłowska-Tylingo
- M. Roślik
- K. Rząd
- series: C-1305, C-1311
The datasets contain the results of determining the minimum inhibitory concentration of acridone derivatives against C. albicans ATCC 10231, C. glabrata ATCC 90030, C. krusei ATCC 6258 and C. parapsilosis ATCC 22019 by the modified M27-A3 specified by the CLSI.
Determination of the minimum inhibitory concentration of C-1330 derivatives (IKE9-IKE14) against Candida strains
Open Research Data
open access
- K. Rząd
- O. Panuś
- I. Gabriel
- E. Paluszkiewicz
- K. Kozłowska-Tylingo
- series: C-1305, C-1311
The datasets contain the results of determining the minimum inhibitory concentration of C-1330 derivatives (IKE9-IKE14) against C. albicans ATCC 10231, C. glabrata ATCC 90030, C. krusei ATCC 6258 and C. parapsilosis ATCC 22019 by the modified M27-A3 specified by the CLSI.
IKE1-IKE3 (C-1305 derivatives) inhibitory effect of the Yeast Topoisomerase II relaxation activity
Open Research Data
version 2.0 open access
- series: C-1305, C-1311
Inhibition of Yeast Topoisomerase II were analyzed according to relaxation assay kit from Inspiralis. Briefly, 250 ng of supercoiled pBR322 DNA, 1 mM ATP, 1-200 μM of analyzed compound were mixed with reaction buffer (1 mM Tris-HCl (pH 7.9), 10 mM KCl, 0.5 mM MgCl 2, 0.2 % (v/v) glycerol). The reaction was initiated by the addition of an enzyme, allowed...
Determination of the minimum inhibitory concentration of new bisacridines IKE15-19 and IKE21, against yeast strains
Open Research Data
open access
- series: Bisacridines
The datasets contain the results of determining the minimum inhibitory concentration of new bisacridines against C. albicans ATCC 10231, C. glabrata ATCC 90030, C. krusei ATCC 6258 and C. parapsilosis ATCC 22019, S. cerevisiae ATCC 9763 and fluconazole resistant C. albicans strains by the modified M27-A3 specified by the CLSI.
Determination of the MIC (minimum inhibitory concentration) of new bisacridines IKE16-19, IKE21 and IE10 against C. glabrata clinical strains
Open Research Data
open access
- W. Jaroszewska
- K. Rząd
- I. Gabriel
- E. Paluszkiewicz
- K. Kozłowska-Tylingo
- series: Bisacridines
The datasets contain the results of determining the MIC value (minimum inhibitory concentration) of new bisacridines IKE16-19, IKE21 and IE10 against Candida glabrata clinical strains CZD 310, 373, 377, 513 and collection strain DSM 11226 by the modified M27-A3 specified by the CLSI.
Western blot analysis of autophagy-related proteins of A549, H226 and H460 cells after exposure to C4
Open Research Data
open access
- N. Maciejewska
The dataset includes measurements of the density of autophagy-related proteins in A549, H226, and H460 cell lines following exposure to compound C4.
Western blot analysis of DDR- and apoptosis-related proteins of A549 and H460 cells after exposure to TXT2 and TXT4
Open Research Data
open access
- N. Maciejewska
The data set contains full-length blot images of DDR- and apoptosis-related proteins of A549 and H460 cells after exposure to TXT2 and TXT4.
Determination of the minimum inhibitory concentration of C-1311 derivatives (C-1296, C-1410, Compound 1, Compound 1-R8) against Candida strains
Open Research Data
open access
- I. Gabriel
- E. Paluszkiewicz
- K. Kozłowska-Tylingo
- K. Rząd
- D. Neubauer
- W. Kamysz
- series: C-1305, C-1311
The datasets contain the results of determining the minimum inhibitory concentration of imidazoacridinone derivatives against C. albicans ATCC 10231, C. glabrata ATCC 90030, C. krusei ATCC 6258 and C. parapsilosis ATCC 22019 and C. albicans clinical strains by the modified M27-A3 specified by the CLSI.
Potential diagnostic utility of recombinant Toxoplasma gondii proteins for detecting anti-Toxoplasma gondii IgG antibodies in horses serum samples
Open Research Data
restricted access
- B. Ferra
Toxoplasmosis is caused by an intracellular protozoan, Toxoplasma gondii, and is a parasitic disease that occurs in all warm-blooded animals, including humans. Toxoplasmosis is one of the most common parasitic diseases of animals and results in reproductive losses. Diagnosis of toxoplasmosis is currently based on serological assays using native antigens...
Potential diagnostic utility of recombinant Toxoplasma gondii proteins for detecting anti-Toxoplasma gondii IgG antibodies in goat serum samples
Open Research Data
restricted access
- B. Ferra
Toxoplasmosis is caused by an intracellular protozoan, Toxoplasma gondii, and is a parasitic disease that occurs in all warm-blooded animals, including humans. Toxoplasmosis is one of the most common parasitic diseases of animals and results in reproductive losses. Diagnosis of toxoplasmosis is currently based on serological assays using native antigens...
Potential diagnostic utility of recombinant Toxoplasma gondii proteins for detecting anti-Toxoplasma gondii IgG antibodies in feline serum samples
Open Research Data
restricted access
- B. Ferra
Toxoplasmosis is caused by an intracellular protozoan, Toxoplasma gondii, and is a parasitic disease that occurs in all warm-blooded animals, including humans. Toxoplasmosis is one of the most common parasitic diseases of animals and results in reproductive losses. Diagnosis of toxoplasmosis is currently based on serological assays using native antigens...
The microstructure of AA5754 alloy under corrosion in alkaline media (pH=11) with 4.5 mM of various carboxylic acids
Open Research Data
open access
This dataset contains scanning electron microscopy (SEM) micrographs taken for polished aluminum AA5754 alloy samples, exposed for 100 min in bicarbonate buffer (pH=11) with the addition of 4.5 mM of carboxylic acids, namely: citric acid, malic acid, maleic acid, succinic acid, tartaric acid, tricarballylic acid and serine. The goal of this study was...
The microstructure of AA5754 alloy under corrosion in alkaline media (pH=11) with 20 mM of various carboxylic acids
Open Research Data
open access
This dataset contains scanning electron microscopy (SEM) micrographs taken for polished aluminum AA5754 alloy samples, exposed for 100 min in bicarbonate buffer (pH=11) with the addition of 20 mM of carboxylic acids, namely: citric acid, malic acid, maleic acid, succinic acid, tartaric acid, tricarballylic acid and serine. The goal of this study was...
L-Homoserine O-acetyltransferase (CaMet2p) activity and inhibition
Open Research Data
open access
The dataset contains the description of methodology and data files with the results obtained spectrophotometrically at 412 nm to determine the rate of fungal enzyme activity. The goal of the study was to obtain conditions optimal for L-homoserine O-acetyltransferase (Met2p) activity as well as evaluation of inhibitory potential of commercially available...
Evaluation of antimicrobial properties of thermomelt adhesives modified with zinc compounds
Open Research Data
open access
The dataset contains the results of microbiological tests of thermomelt adhesives modified with zinc compounds, whose activity was assessed for their ability to reduce the number of Escherichia coli and Staphylococcus aureus strain, representing the Gram (-) and Gram (+) bacteria, respectively. The evaluation of antimicrobial properties of samples were...
AFM images of the platinum (111) single crystal surface as delivered, without the polishing treatment
Open Research Data
open access
- A. Zieliński
- J. Ryl
- series: Scanning Probe Microscopies data
Platinum (111) single crystal surface images as delivered, without polishing treatment. Topographic measurements in contact mode. NTEGRA Prima (NT-MDT) device. CSG probe 10.
Dynamic impedance data for various carboxylic acids corrosion inhibition of aluminium in alkaline media (pH=11)
Open Research Data
open access
This dataset contains the results of Dynamic Electrochemical Impedance Spectroscopy (DEIS) carried out in galvanostatic mode under iDC=0, carried out in order to define the corrosion inhibition effect of various studied carboxylic acids: citric acid, malic acid, maleic acid, succinic acid, tartaric acid, carballylic acid, and serine. The experiment...
Construction of recombinant plasmid coding Borrelia burgdorferi sl B1 protein
Open Research Data
embargo
- L. Holec-Gąsior
- series: Borrelia burgdorferi sl recombinant B1 protein
Diagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
Construction of recombinant plasmid coding Borrelia burgdorferi sl B6 protein
Open Research Data
embargo
- L. Holec-Gąsior
- series: Borrelia burgdorferi sl recombinant B6 protein
Diagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
Construction of recombinant plasmid coding Borrelia burgdorferi sl B2 protein
Open Research Data
embargo
- L. Holec-Gąsior
- series: Borrelia burgdorferi sl recombinant B2 protein
Diagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
Construction of recombinant plasmid coding Borrelia burgdorferi sl B12 protein
Open Research Data
embargo
- L. Holec-Gąsior
- series: Borrelia burgdorferi sl recombinant B12 protein
Diagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
Construction of recombinant plasmid coding Borrelia burgdorferi sl B3 protein
Open Research Data
embargo
- L. Holec-Gąsior
- series: Borrelia burgdorferi sl recombinant B3 protein
Diagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
Determination of the effective concentration of ketoprofen against the germination of Sorghum bicolor (sorghum) seeds
Open Research Data
open access
- series: Impact of selected drugs and their binary mixtures on the germination of Sorghum bicolor (sorgo) seeds
Research data includes an attempt to determine the effective concentration of ketoprofen that inhibits germination of Sorghum bicolor (sorghum) seeds.
X-ray Photoelectron Spectroscopy studies of various carboxylic acids adsorption on aluminium alloys in alkaline media
Open Research Data
open access
This dataset contains the results of high-resolution XPS studies obtained during evaluation of high corrosion inhibition efficiency of various carboxylic acids towards aluminium alloy 5754 in bicarbonate buffer pH=11.
Construction of recombinant plasmid coding Borrelia burgdorferi sl P protein
Open Research Data
embargo
- L. Holec-Gąsior
- K. Sołowińska
- series: Borrelia burgdorferi sl recombinant P protein
Diagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
Construction of recombinant plasmid coding Borrelia burgdorferii sl F protein
Open Research Data
embargo
- L. Holec-Gąsior
- K. Sołowińska
- series: Borrelia burgdorferi sl recombinant F protein
Diagnosis of Lyme borreliosis (LB), an infection caused by spirochaetes of the B. burgdorferi sl species complex, is mainly based on clinical symptoms supported with serology and is often misdiagnosed in areas of endemicity. The complexity of the antigenic composition among the Borrelia genospecies and differential expression of proteins in host and...
Toxoplasma godnii recombinant chimeric antigens - IgM and IgG ELISAs - mouse serum samples
Open Research Data
open access
- B. Ferra
This study presents an evaluation of tetravalent recombinant chimeric proteins containing fragments of the Toxoplasma gondii antigens, SAG2, SAG1, GRA1, GRA2, MIC1, MAG1, ROP1 and AMA1, as potential replacements of a the soluble, whole-cell tachyzoite lysate (TLA) used in serological assays. Recombinant chimeric proteins (SAG1-MIC1-MAG1-GRA2, SAG2-GRA1-ROP1-GRA2,...
Toxoplasma gondii recombinant chimeric antigens - IgG and IgM ELISAs - human serum samples
Open Research Data
open access
- B. Ferra
This study presents an evaluation of tetravalent recombinant chimeric proteins containing fragments of the Toxoplasma gondii antigens, SAG1, SAG2, GRA1, GRA2, MIC1, MAG1, ROP1 and AMA1, as potential replacements of a the soluble, whole-cell tachyzoite lysate (TLA) used in serological assays. Recombinant chimeric proteins (SAG1-MIC1-MAG1-GRA2, SAG2-GRA1-ROP1-GRA2,...
Data on solutions of Hes1 system
Open Research Data
open access
- A. Bartłomiejczyk
- M. Bodnar
- G. Graff
Hes1 protein (hairy and enhancer of split 1) belongs to the helix-loop-helix (bHLH) family of transcription proteins, i.e. DNA-binding proteins in the promoter region or in another region where regulation of transcription processes occurs. The database collects data on solutions of the Hes1 systems with multiple binding sites and the dimer formation...
Impact of binary mixtures of methylparaben and buthylparaben on endocrine processes of the model organism Saccharomyces Cerevisiae (using XenoScreen YES/YAS test) - with controls
Open Research Data
open access
- series: Ecotoxicological equilibria of triclosan in Microtox, XenoScreen YES/YAS, Caco2, HEPG2 and liposomal systems are affected by the occurrence of other pharmaceutical and personal care emerging contaminants
Data shown in data set illustrate the results of the endocrine processes of mixtures of methylparaben and buthylparaben with controls for YES agonsit, YES antagonist, YAS agonist and YAS antagonist. S cerevisiae is used as a model organism in the monitoring of endocrine disruptors in environmental and reference samples in Xenometrix® systems - XenoScreen®....
Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen AMA1-MIC1-MIC3
Open Research Data
version 1.1 restricted access
- B. Ferra
The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give...
Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen MIC3-MAG1-ROP1
Open Research Data
version 1.1 restricted access
- B. Ferra
The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give...
Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen MIC1-MIC3-ROP1
Open Research Data
version 1.1 restricted access
- B. Ferra
The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give...
Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen MIC3-MAG1-AMA1
Open Research Data
version 1.1 restricted access
- B. Ferra
The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give...
Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen AMA1-MIC1-ROP1
Open Research Data
version 1.1 restricted access
- B. Ferra
The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give...
Phoenixin-14 effect on viability and THBS2 and CDH1 expression in 12Z endometriosis cell line
Open Research Data
open access
- K. Kulińska
- M. Wierzbicka
- M. Andrusiewicz
- M. Wołuń-Cholewa
Phoenixin-14 is a neuropeptide produced by the paraventricular nuclei of the anterior hypothalamus that regulates reproductive system functions. Its level in patients with endometriosis is reduced and may be the cause of dysregulated HPG-axis. Our study tested endometriosis-specific concentrations of PNX-14 on endometrial epithelial cell line 12Z in...
Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen MIC1-MAG1-MIC3
Open Research Data
version 1.1 restricted access
- B. Ferra
The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give...
Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen MIC1-MAG1-ROP1
Open Research Data
version 1.1 restricted access
- B. Ferra
The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give...
Construction, production and evaluation of the diagnostic utility of a recombinant Toxoplasma gondii chimeric antigen MIC1-MAG1-AMA1
Open Research Data
version 1.1 restricted access
- B. Ferra
The intracellular parasite Toxoplasma gondii has the ability to infect a wide range of warm-blooded animals, including humans. Currently, diagnosis of toxoplasmosis is based mainly on the use of the native antigens in enzyme immunoassay which allow for detection of IgG, IgM and IgA antibody classes. However, in some cases the performed studies give...
Plasmids pMET2M1 and pMET15M1 with the SAT1 Flipper knockout cassette construction
Open Research Data
open access
- K. Rząd
- I. Gabriel
The goal of the study was to obtain plasmids containing the SAT1 Flipper knockout cassette along with upstream and downstream gene fragments (MET2, MET15) and to introduce a cleavage site for restriction enzymes into them.

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Search results for: PROTEINASE INHIBITORS