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Search results for: INHIBICJA ENZYMÓW
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Isolation and some properties of the thermostable beta-galactosidase of Pyrococcus woesei expressed in Escherichia coli
PublicationOpracowano metodę izolowania oraz zbadano właściwości enzymu otrzymanego ze szczepu Escherichia coli zawierającego gen kodujący termostabilną beta-ga-laktozydazę z Pyrococcus woesei.
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Elucidation of chemical compounds in different extracts of two Lavandula taxa and their biological potentials: Walking with versatile agents on the road from nature to functional applications
PublicationDue to their health-promoting and nutraceutical properties, lavenders are frequently employed in the food and pharmaceutical industries. Here, we focused on investigate the chemical profile of hexane, MeOH, and water extracts of leaf, flower, and root of L. pedunculata and L. stoechas and to assess their antioxidant, enzyme inhibitory, and antibacterial properties. The highest total polyphenolic and flavonoids contents were recorded...
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In silico design of telomerase inhibitors.
PublicationTelomerase is a reverse transcriptase enzyme involved in DNA synthesis at the end of linear chromosomes. Unlike in most other cells, telomerase is reactivated most cancerous cells and, therefore, has become a promising new anticancer target. Despite extensive research, direct telomerase inhibitors have yet not been introduced to the clinics because of the complexity of this enzyme. Structures of this protein from simple organisms...
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Oxygen biosensor based on carbon nanotubes directly grown on graphitic substrate
PublicationFilms of carbon nanotubes were synthesized on the graphitic substrates by CVD method in the presence of catalyst. Two different catalysts were used, that is nickel and iron, supported on graphitic substrates. The as-obtained nanotubes were analyzed by SEM imaging with EDX and Raman spectroscopy. The results revealed their multi-walled morphology. Free radical reaction was carried out in order to chemically functionalize synthesized...
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Comparative molecular dynamics study of dimeric and monomeric forms of HIV-1 protease in ligand bound and unbound state
PublicationHuman immunodeficiency virus type 1 protease (HIV-1 PR) is a viral-encoded enzyme that forms a homodimer. HIV-1 PR is essential for replication and assembly of the virus and inactivation of HIV-1 PR enzyme causes production of immature, noninfectious viral particles and thus HIV-1 PR is an attractive target in anti-AIDS drug design. In our current work, we performed molecular dynamics (MD) calculations (500 ns) for two different...
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Modulation of UDP-glucuronidation by acridinone antitumor agents C-1305 and C-1311 in HepG2 and HT29 cell lines, despite slight impact in noncellular systems.
PublicationBackground Among the studied antitumor acridinone derivatives developed in our laboratory, 5-dimethylaminopropylamino-8-hydroxytriazoloacridinone (C-1305) and 5-diethylaminoethylamino-8-hydroxyimidazoacridinone (C-1311) exhibited cytotoxic and antitumor properties against several cancer types and were selected to be evaluated in preclinical and early-phase clinical trials. In the present work, we investigated the impact of C-1305...
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Active Site Architecture and Reaction Mechanism Determination of Cold Adapted beta-D-galactosidase from Arthrobacter sp. 32cB
PublicationArthbetaDG is a dimeric, cold-adapted beta-D-galactosidase that exhibits high hydrolytic and transglycosylation activity. A series of crystal structures of its wild form, as well as its ArthbetaDG_E441Q mutein complexes with ligands were obtained in order to describe the mode of its action. The ArthbetaDG_E441Q mutein is an inactive form of the enzyme designed to enable observation of enzyme interaction with its substrate. The...
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Elucidating the impact of enzymatic modifications on the structure, properties, and applications of cellulose, chitosan, starch and their derivatives: a review
PublicationPolysaccharides, as one of the most prominent natural biopolymers, display numerous biological activities and industrial applications. Nevertheless, some polysaccharides lack biological properties or display weak biological activities. Besides, practical usages of polysaccharides still remain challenging because of their poor solubility and inappropriate hydrophilic/hydrophobic balance. Hence, enzymatic or chemical modifications...
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Uciążliwość odorowa zakładów przetwórstwa ryb
PublicationPrzetwórstwo rybne jest uważane za jedną z najszybciej rozwijających się gałęzi sektora żywnościowego w Polsce, a duże rozmiary produkcji wiążą się ściśle z powstawaniem znaczących ilości odpadów. Świeży surowiec rybny charakteryzuje się niezbyt intensywnym, swoistym zapachem, natomiast bardzo szybko ulega zepsuciu w wyniku działania endogennych enzymów i mikroflory bakteryjnej, generując lotne związki o odrażającej woni. Nawet...
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Structure of EstA esterase from psychrotrophic Pseudoalteromonas sp. 643A covalently inhibited by monoethylphosphonate.
PublicationThe crystal structure of the esterase EstA from the cold-adapted bacteriumPseudoalteromonas sp. 643A was determined in a covalently inhibited form at aresolution of 1.35 A˚. The enzyme has a typical SGNH hydrolase structureconsisting of a single domain containing a five-stranded beta-sheet, with threehelices at the convex side and two helices at the concave side of the sheet, and isornamented with a couple of very short helices...
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Antifungal Activity of Homoaconitate and Homoisocitrate Analogs
PublicationThirteen structural analogs of two initial intermediates of the L-alpha-aminoadipate pathway of L-lysine biosynthesis in fungi have been designed and synthesized, including fluoro- and epoxy-derivatives of homoaconitate and homoisocitrate. Some of the obtainedcompounds exhibited at milimolar range moderate enzyme inhibitory properties against homoaconitase and/or homoisocitrate dehydrogenase of Candida albicans. The structural...
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Nowe estrowe i amidowe pochodne inhibitorów syntazy glukozamino-6-fosforanu - synteza i aktywność biologiczna
PublicationPrzedmiotem rozprawy doktorskiej są modyfikacje chemiczne analogów glutaminy – kwasu N3-(4-metoksyfumaroilo)-S-2,3-diaminopropanowego (FMDP) oraz kwasu N3-[(E)-4-fenylo-4-okso-2-butenoilo]-S-2,3-diaminoaminopropanowego (BADP). Związki te są inhibitorami syntazy glukozamino-6-fosforanu i mimo ich silnych właściwości inhibicyjnych, nie wykazują zdolności do hamowania wzrostu drobnoustrojów. Brak aktywności przeciwdrobnoustrojowej...
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Determination of phenol biodegradation pathways in three psychrotolerant yeasts, Candida subhashii A011, Candida oregonensis B021 and Schizoblastosporion starkeyi-henricii L012, isolated from Rucianka peatland
PublicationIn this study, three psychrotolerant phenol-degrading yeast strains Candida subhashii (strain A011), Candida oregonenis (strain B021) and Schizoblastosporion starkeyi-henricii (strain L012) isolated from Rucianka peatland were examined to determine which alternative metabolic pathway for phenol biodegradation is used by these microorganisms. All yeast strains were cultivated in minimal salt medium supplemented with phenol at 500,...
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Immobilization of thermostable beta glucosidase from Sulfolobus shibatae by cross-linking with transglutaminase
PublicationOpracowano metodę immobilizacji termostabilnej beta-glukozydazy przez katalizowane transglutaminazą sieciowanie na nośniku krzemionkowym. Aktywność uzyskanego preparatu wynosi 3883 U/g nośnika. Największą wydajność unieruchamiania uzyskano w temperaturze 50C, przy pH 5,0. Immobilizacja prawie nie zmieniała optymalnego pH działania enzymu, który uzyskiwał największą aktywność w temperaturze 98C. Badania w reaktorze kolumnowym wykazały...
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Induction of secondary metabolite production in transformated callus of Ammi majus L. grown after electromagnetic treatment of culture medium
PublicationW pracy przedstawiono wyniki badań, których celem było zbadanie wpływu elicytacji przy użyciu ADR-4, na produkcję metabolitów wtórnych. Elicytacja ADR-4 zwiększyła 2-krotnie zawartość bergaptenu. Porównano również efektywność 3 technik ekstrakcyjnych stosowanych do izolacji feuranokumaryn, kumaryn i ich glikozydów z kalusa A.majus. Ekstrakcja przy użyciu techniki ASE okazała się najbardziej efektywna.
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Stimulation of antibacterial napthoquinones and flavonoids accumulation in carnivorous plants grown in vitro by addition of elicitors
PublicationRośliny owadożerne (D. muscipula, D. capensis) zawierają dwie grupy farmakologicznie czynnych substancji - naftochinony: plumbagina, ramentaceon, oraz flawonoidy: kwercetyna i mirycetyna. Na wzrost akumulacji tychże substancji w tkankach roślin owadożernych ma wpływ dodatek elicytora. Zbadano wpływ następujących elicytorów na zawartość metabolitów o znaczeniu farmaceutycznym: kwas jaśminowy oraz lizat Agrobacterium rhizogenes....
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Antarctic, cold-adapted beta-galactosidase of Pseudoalteromonas sp. 22b as an effective tool for alkyl galactopyranosides synthesis
PublicationPrzedstawione jest zastosowanie arktycznej beta-galaktozydazy Pseudoalteromonas sp. 22b do katalizy syntezy alkilowych galaktopyranozydów. Njwyższą wydajność syntezy uzyskuje się przy pH 6-9 i 10-30% stężenia wody. Podobnie jak dla mezofilnych beta-galaktozydaz enzym antarktyczny syntetyzował najwydajniej kiedy reakcja przebiega w mieszaninie buforu i rozpuszczalnika organiczego (poniżej 50%, v/v).
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Structural studies of a cold-adapted dimeric Beta-D-galactosidase from Paracoccus sp. 32d
PublicationThe crystal structure of a novel dimeric [beta]-D-galactosidase from Paracoccus sp. 32d (Par[beta]DG) was solved in space group P212121 at a resolution of 2.4 Å by molecular replacement with multiple models using the BALBES software. This enzyme belongs to glycoside hydrolase family 2 (GH2), similar to the tetrameric and hexameric [beta]-D-galactosidases from Escherichia coli and Arthrobacter sp. C2-2, respectively. It is the second...
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Desensitization of glucosamine-6-phosphate synthase to inhibition by UDP-GlcNAc
PublicationGlucosamine-6-phosphate (GlcN-6-P) synthase, known also as L-Glutamine: D-fructose-6-phosphate amidotransferase, catalyzes the first committed step in the pathway leading to the ultimate formation of UDP-GlcNAc. The final product of this pathway is an activated precursor of numerous macromolecules containing amino sugars, including chitin and mannproteins in fungi, peptidoglycan and lipopolysaccharides in bacteria, and glycoproteins...
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Cloning, expression in Komagataella phaffii, and biochemical characterization of recombinant sequence variants of Pseudomonas sp. S9 GDSL-esterase
PublicationTwo recombinant Komagataella phaffii (formerly Pichia pastoris) yeast strains for production of two sequential variants of EstS9 esterase from psychrotolerant bacterium Pseudomonas sp. S9, i.e. αEstS9N (a two-domain enzyme consisting of a catalytic domain and an autotransporter domain) and αEstS9Δ (a single-domain esterase) were constructed. However, only one of recombinant K. phaffii strains, namely Komagataella phaffii X-33/pPICZαestS9Δ,...
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Investigating the disease- modifying properties of sclerotiorin in Alzheimer's therapy using acetylcholinesterase inhibition
PublicationAlzheimer's disease (AD) is a progressive neurodegenerative disorder caused due to the damage and loss of neurons in specific brain regions. It is the most common form of dementia observed in older people. The symptoms start with memory loss and gradually cause the inability to speak and do day-to-day activities. The cost of caring for those affected individuals is huge and is probably beyond most developing countries capability....
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Modification of quaternary structure of Candida albicans GlcN-6-P synthase and its desensitization to inhibition by UDP-GlcNAc by site-directed mutagenesis
PublicationSite-directed mutagenesis of the CaGFA1 gene encoding glucosamine-6-phosphate synthase from Candida albicans was performed. Desensitization of the enzyme to inhibition by UDPGlcNAc was achieved upon T487I and H492F substitutions at the UDP-GlcNAc binding site, exchange of D524, S525 and S527 for Ala at the dimer:dimer interface and construction of the tail-lock array (L434R and L460A) at the C-tail region. The first two sets if...
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The Caucasian flora: a still-to-be-discovered rich source of antioxidants
PublicationCellular redox homeostasis is a state of balance between the formation of Usually Reactive Oxygen and / or Nitrogen Species (ROS/RNS), endogenous antioxidant defence systems, and exogenous dietary antioxidants. The disturbance of redox homeostasis, by the overproduction of endogenous ROS/RNS, may increase the risk of development of so-called civilisation diseases. The solution seems to be either the increased production of endogenous...
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Molecular Targets for Anticandidal Chemotherapy
PublicationA relatively small number of anticandidal chemotherapeutics used in clinical practice is at least in part consequence of a limited number of their molecular targets: ergosterol in the membrane, lanosterol demethylase, b(1!3) glucan synthase, and DNA/RNA biosynthesis. Much more potential novel targets have been revealed by the comparative genomic studies identifying essential genes unique for Candida albicans or resulted from recognition...
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Przydatność białka rybiego w postaci kolagenu lub żelatyny oraz polisacharydu - kapa-karagenu do wytwarzania aktywnych opakowań biodegradowalnych = Usefulness of fish collagen, gelatin and carrageenan for preparation of active biodegradable packages
PublicationSummary - The possibility of use of protein films made of fish collagen or gelatin as well as polysaccharide carrageenan films as carriers of enzymes (lysozyme or lysostaphyne), for preparation of microbiologically active packages, was investigated. It was found that crosslinking of such systems with N-[3(dimethylamino)propyl]-N'-ethylcarboimide (EDC) does not influence the activity of lysozyme immobilized in the films (Table 1)....
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Targeting DNA Topoisomerase II in Antifungal Chemotherapy.
PublicationTopoisomerase inhibitors have been in use clinically for the treatment of several diseases for decades. Although those enzymes are significant molecular targets in antibacterial and anticancer chemotherapy very little is known about the possibilities to target fungal topoisomerase II (topo II). Raising concern for the fungal infections, lack of effective drugs and a phenomenon of multidrug resistance underlie a strong need to expand...
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Methyl 3-[(1,1-dioxo-1λ<sup>6</sup>,2-benzothiazol- 3-yl)amino]-5-nitrothiophene-2-carboxylate
PublicationTytułowy związek został otrzymany przez nitrowanie odpowiedniej pochodnej tiofenu. Wyznaczono i opisano jego strukturę rentgenowską w ciele stałym. Związek wykazuje pewne właściwości inhibicyjne w stosunku do enzymu ludzkiej elastazy leukocytowej (HLE).
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Techniki chromatografii cieczowej, stosowane w analityce technicznej procesów hydrolizy biomasy ligno-celulozowej - BMLC. Przegląd
PublicationObróbka wstępna biomasy ligno-celulozowej (BMLC) realizowana z zastosowaniem kwasów, zasad oraz enzymów jest ważnym etapem przetwarzania surowca przed procesem fermentacji. Podczas hydrolizy złożone struktury biopolimerów: celulozy, hemicelulozy i ligniny rozpadają się do związków o małej masie cząsteczkowej (cukry, oligosacharydy, związki fenolowe). Jednak nie jest to proces łatwy i wymaga doboru odpowiednich warunków procesu,...
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Zmiany zachodzące w komórkach mikroorganizmów pod wpływem podwyższonego ciśnienia
PublicationMechanizm śmierci mikroorganizmów w warunkach wysokociśnieniowych jest bardzo złożony i nie został w pełni wyjaśniony. Ciśnienia rzędu 5-40 MPa zazwyczaj nie prowadzą jeszcze do śmierci komórek, lecz powodują zmiany ich kształtu i wymiarów lub ruchliwości. W przypadku niektórych mikroorganizmów, głównie grzybów, wydłużenie komórki może prowadzić do mechanicznego rozerwania naprężonej ściany komórkowej i w efekcie do inaktywacji...
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Fusion of DNA-binding domain of Pyrococcus furiosus ligase with TaqStoffel DNA polymerase as a useful tool in PCR with difficult targets
PublicationThe DNA coding sequence of TaqStoffel polymer- ase was fused with the DNA-binding domain of Pyrococcus furiosus ligase. The resulting novel recombinant gene was cloned and expressed in E. coli. The recombinant enzyme was purified and its enzymatic features were studied. The fusion protein (PfuDBDlig-TaqS) was found to have enhanced processivity as a result of the conversion of the Taq DNA polymerase from a relatively low processive...
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Inhibitors of glucosamine-6-phosphate synthase as potential antimicrobials or antidiabetics – synthesis and properties
PublicationGlucosamine-6-phosphate synthase (GlcN-6-P synthase) is known as a promising target for antimicrobial agents and antidiabetics. Several compounds of natural or synthetic origin have been identified as inhibitors of this enzyme. This set comprises highly selective L-glutamine, amino sugar phosphate or transition state intermediate cis-enolamine analogues. Relatively low antimicrobial activity of these inhibitors, poorly penetrating...
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High level expression, secretion and purification of the termostable aqualysin I from Thermus aquaticus YT-1 in Pichia pastoris.
PublicationOtrzymano aktywną proteazę serynową Thermus aquaticus z systemu nadprodukcji Escherichia coli. Oczyszczono enzym przy zastosowaniu chromatografii metalo-powinowactwa.
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Investigation of the antimicrobial potential and microbiota of bee pollen and bee bread derived from Polish apiaries
PublicationThe phenomenon of resistance of microorganisms to antibiotics is becoming one of the greatest challenges of modern medicine. At the same time, the number of new antibiotics introduced into clinical practice is decreasing. This creates the need to search for new, effective, non-antibiotic methods of combating and preventing infectious diseases. Over the last two decades, there has been renewed interest in the antimicrobial potential...
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Protective effect of lysostaphin from Staphylococcus simulans against growth of Staphylococcus aureus in milk and some other food products
PublicationZbadano skuteczność hamowania rozwoju Staphylococcus aureus w mleku, mięsie wieprzowym i majonezie lizostafyną uzyskaną przez ekspresję genu enzymu ze Staphylococcus simulans w komórkach Escherichia coli. Przeprowadzone eksperymenty wykazały znaczący wpływ lizostafyny na ograniczenie rozwoju Staphylococcus aureus.
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Differential ability of cytostatics from anthraquinone group to generatefree radicals in three enzymatic systems: NADH dehydrogenase, NADPH cytochrome P450 reductase, and xantine oxidase.
PublicationZbadano szereg cytostatyków z grupy antrachinonu pod względem ich zdolności do pośredniczenia w przeniesieniu jednoelektronowym i generowaniu wolnych rodników tlenowych pod wpływem trzech oksydoreduktaz. Zdefiniowano czynniki strukturalne związków odpowiedzialne za ten proces katalizowany przez poszczególne enzymy.
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The role of glucuronidation in drug resistance
PublicationThe final therapeutic effect of a drug candidate, which is directed to a specific molecular target strongly depends on its absorption, distribution, metabolism and excretion (ADME). The disruption of at least one element of ADME may result in serious drug resistance. In this work we described the role of one element of this resistance: phase II metabolism with UDP-glucuronosyltransferases (UGTs). UGT function is the transformation...
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Purification and biochemical characteristic of a cold-active recombinant esterase from Pseudoalteromonas sp. 643A under denaturing conditions.
PublicationIn this paper production of a cold-active esterase EstA from the Antarctic bacterium Pseudoalteromonas sp. 643A in E. coli expression system was described. The purification and biochemical characteristic of EstA were performed in the presence of urea and then compared with results obtained for the esterase with no addition of urea and isolated from the native source. In both cases the cold-active enzyme displayed similar properties....
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Modulation of carcinogen metabolizing cytochromes p450 in rat liver and kidney by cabbage and sauerkraut juices: comparison with the effects of indole-3-carbinol and phenethyl isothiocyanate
PublicationThis study investigated the effect of raw cabbage and sauerkraut juices on the activity and expression of CYP1A1, 1A2, 1B1 and 2B in Wistar rat liver and kidney. The results were compared with those of two commercially available products of glucosinolates degradation: indole-3-carbinol (I3C) and phenethyl isothiocyanate (PEITC). Significant differences in the effect of the cabbage juices as well as I3C and PEITC between the liver...
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The role of intestine and liver in glucuronidation of the imidazo- and triazoloacridinone antitumor agents, C-1311 and C-1305: High affinity substrates for UGT1A10
PublicationCelem przeprowadzonych badań było określenie roli poszczególnych izoform UDP-glukuronylotransferazy (UGT)oraz mikrosomów jelita(HIM)i wątroby (HLM) wyizolowanych od różnych pacjentów w metabolizmie pochodnych akrydonu, związków C-1311 i C-1305. Wykazano, że rodziną UGT katalizującą przemiany C-1305 i C-1311 jest UGT1A, z najwyższą aktywnością wykazywaną przez pozawątrobowy izoenzym UGT1A10. Rozkład aktywności frakcji mikrosomalnych...
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Badanie wpływu struktury białek pokarmowych na kinetykę ich trawienia
PublicationW dzisiejszych czasach coraz więcej ludzi cierpi na choroby cywilizacyjne związane z nieprawidłowym odżywianiem, takie jak: otyłość, cukrzyca, schorzenia układu krwionośnego, czy sarkopenia. Naukowcy z całego świata starają się znaleźć rozwiązania mające na celu zapobieganie wspomnianym problemom. Jedna z metod, która w tym pomaga, wykorzystuje modele symulujące warunki fizjologiczne ludzkiego układu pokarmowego w warunkach in...
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Acridinone antitumor agents, C-1305 and C-1299 are metabolized rather by flavin-containing monooxygenases and UDP-glucuronosyltransferases than cytochromes P450
PublicationStwierdzono, że to monooksygenazy flawinowe, a nie enzymy cytochromu P450, są głównymi enzymami frakcji mikrosomalnej, odpowiedzialnymi za detoksyfikacyjny metabolizm dwóch badanych pochodnych triazoloakrydonu. Dodatkowo C-1305 ulega sprzęganiu w czasie drugiej fazy metabolizmu z kwasem glukuronowym do postaci O-glukuronidu
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Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans—Expression and characterization
PublicationDNA polymerases are present in all organisms and are important enzymes that synthesise DNA molecules. They are used in various fields of science, predominantly as essential components for in vitro DNA syntheses, known as PCR. Modern diagnostics, molecular biology and genetic engineering need DNA polymerases which demonstrate improved performance. This study was aimed at obtaining a new NeqSSB-TaqS fusion DNA polymerase from the...
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DNA restriction analysis as a supportive tool in mechanistic studies carried out by 32P-postlabelling
PublicationNumerous antitumor and carcinogenic compounds are able to modify DNA by forming covalent bonds with its constituents, while some anticarcinogenic compounds are known to prevent such a modification. All these processes are of vital biological import_ance, though deeper inside into factors influencing formation of DNA adducts is difficult due to the low level of their occurrence. 32P-Postlabelling approach ensures very sensitive...
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Covalent DNA modification by products of myrosinase catalysed glucosinolate degradation in cell-free system
PublicationThe bioactive phytochemicals found in Brassica vegetables belonging to glucosinolates (GLS) and especially the products of their degradation isothiocyanates (ITC) and indoles are regarded as the most promising cancer chemopreventive compounds. These secondary metabolites constitute defence system repelling or preventing the development of agrophages attacking brassica plants. The antibiological properties of these compounds suggest...
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The influence of food phytochemicals on cyclic phosphodiesterase 4 (PDE4) activity
PublicationCyclic phosphodiesterase 4 (PDE4) belongs to family of cyclic phosphodiesterases, which are expressed predominantly in inflammatory cells, airway smooth muscles, cardiovascular tissues and brain. Inhibitors of this family of enzymes found medicinal applications as antidepressants, antiinflamatory drugs (mainly in airway diseases) or antiasthmatics (Ibudilast). PDE4 inhibitors are also being tested for preventing the development...
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Lizostafyna - źródła otrzymywania i zastosowanie
PublicationW pracy przedstawiono ogólną charakterystykę liostafyny: budowę, właściwości biochemiczne, źródła otrzymywania enzymu. Przedstawiono także możliwości wykorzystania tego białka w medycynie i weterynarii jako alternatywny chemioterapeutyk przeciw infekcjom wywoływanym przez gronkowce oraz w przemyśle żywnościowym do poprawy mikrobiologicznej jakości produktów.
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Genetycznie modyfikowane mikroorganizmy narzędziem służącym do usuwania metali ciężkich ze środowiska
PublicationMetale ciężkie są pierwiastkami metalicznymi i półmetalicznymi o gęstości powyżej 4,5 g•cm-3. Powodują zaburzenia w strukturze DNA, mogą być przyczyną chorób nowotworowych, układu nerwowego, pokarmowego, naczyniowego i kostnego. Wykorzystywane są w przemyśle chemicznym, elektromaszynowym, szklarskim, metalurgicznym, farbiarskim, wydobywczym, energetyce jądrowej. Wiele metali, takich jak kobalt, miedź, nikiel, stanowi mikroelementy...
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Modulation of CYP1A1, CYP1A2 and CYP1B1 expression by cabbage juices and indoles in human breast cell lines
PublicationEpidemiological studies have shown that consumption of cab- bage and sauerkraut is connected with significant reduction of breast cancer incidences. Estrogens are considered a major breast cancer risk factor and their metabolism by P450 enzymes substan- tially contributes to carcinogenic activity. The aim of this study was to investigate the effect of cabbage and sauerkraut juices of differ- ent origin on the expression profile...
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Long range molecular dynamics study of regulation of eukaryotic glucosamine-6-phosphate synthase activity by UDP-GlcNAc
PublicationGlucosamine-6-phosphate (GlcN-6-P) synthase catalyses the first and practically irreversible step in hexosamine metabolism. The final product of this pathway, uridine 5' diphospho N-acetyl-D-glucosamine (UDPGlcNAc), is an essential substrate for assembly of bacterialand fungal cell walls. Moreover, the enzyme is involved in phenomenon of hexosamine induced insulin resistance in type II diabetes, which makes it a potential target...
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Recombinant !ermostable AP Exonuclease from Thermoanaerobacter tengcongensis: Cloning, Expression, Purification, Properties and PCR Application
PublicationApurinic/apyrimidinic (AP) sites in DNA are considered to be highly mutagenic and must be corrected to preserve genetic integrity, especially at high temperatures. !e gene encoding a homologue of AP exonuclease was cloned from the thermophilic anaerobic bacterium Thermoanaerobacter tengcongensis and transformed into Escherichia coli. The protein product showed high identity (80%) to human Ape1 nuclease, whereas to E. coli exonuclease...