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Search results for: “COLD” EXTRACTION
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Exploring multi-step glucose oxidation kinetics at GOx-functionalized nanotextured gold surfaces with differential impedimetric technique
PublicationFor a few past years, we can observe the enormous growth of investigations related to ultrasensitive electrochemical sensors capable of reliable determination of important body parameters and analytes. Utilized procedures rely on standard electrochemical methods, demanding electrode polarization, and information about the initial characteristics of the working electrode. More and more complex electrode materials are characterized...
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Ultrasensitive electrochemical determination of the cancer biomarker protein sPD-L1 based on a BMS-8-modified gold electrode
PublicationThis work describes the modification of a gold electrode with the BMS-8 compound that interacts with the Programmed Death-Ligand 1 (PD-L1), an immune checkpoint protein. The results show that we can confirm the presence of the sPD-L1 in the concentration range of 10−18 to 10−8 M using electrochemical impedance spectroscopy (EIS) with a limit of detection (LOD) of 1.87 × 10−14 M for PD-L1 (S/N = 3.3) and at a concentration of 10−14...
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Thermal dewetting as a method of surface modification of the gold thin films for surface plasmon resonance based sensor applications
PublicationHere, we report a quick and simple approach with low, optimized production costs to obtain surface plasmon resonance (SPR) based sensors fabricated through a time- and resource-effective method based on thermal dewetting of thin Au films. From the applicative point of view, the method of detection presented here should be easier to implement, since light transmission measurements seem to be much less challenging than light refractive...
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Use of personal knowledge of 8-year-old students in creative solving of technical tasks
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The COVID-19 pandemic in the Middle East and North Africa. Old demons in a new bottle
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Lactose-rich milk replacer modifies the proteome of bloodplasma in 2-week-old calves
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Safety of Bariatric Surgery in ≥ 65-Year-Old Patients During the COVID-19 Pandemic
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Development of potential candidate reference materials for drugs in bottom sediment, cod and herring tissues
PublicationRegular use of a reference material and participation in a proficiency testing program can improve the reliability of analytical data. This paper presents the preparation of candidate reference materials for the drugs metoprolol, propranolol, carbamazepine, naproxen, and acenocoumarol in freshwater bottom sediment and cod and herring tissues. These reference materials are not available commercially. Drugs (between 7 ng/g and 32...
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Sprawy estetyki starych mostów w Tczewie = Aesthetic matters of the old bridges in Tczew
PublicationNa tle dziejów historycznych mostów w Tczewie przeanalizowano sprawy ich estetyki i dziedzictwa wyznaczające krajobraz kultury danego miejsca.
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Environmental Factors and the Risk of Developing Type 1 Diabetes—Old Disease and New Data
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Determination of phytochemical composition and antioxidant capacity of 22 old apple cultivars grown in Poland
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Creation of structures by 4, 5 and 6-year-old children, considering the conditions for the structure
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New Life of an Old Drug: Caffeine as a Modulator of Antibacterial Activity of Commonly Used Antibiotics
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The effect of high pressure on formation of volatile amines in minced meat of cod (Gadus morhua)
PublicationThe effects of high pressure at subzero temperature (193 MPa, at −20 °C) on inactivation of natural microflora of cod meat and degradation of trimethylamine oxide (TMAO) to trimethylamine (TMA) during refrigerated storage and to dimethylamine (DMA) and formaldehyde (FA) during frozen storage were investigated. The content of TMA, DMA and FA in cod meat did not change immediately after pressure treatment. During 40 days of frozen...
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Determination of COD Fractionation as a Key Factor for Appropriate Modelling and Monitoring of Activated Sludge Processes
PublicationAn operation of wastewater treatment plant is usually controlled by global parameters such as flow, solids retention time, sludge age, concentration of ammonia and dissolved oxygen, etc. It is considered that, together with the chemical and biological oxygen demand (COD and BOD), those parameters indirectly exhibit the effectiveness of activated sludge processes. Especially the BOD indicate the amount of organic pollution that...
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Chemical, Physical, and Mechanical Properties of 95-Year-Old Concrete Built-In Arch Bridge
PublicationThis research aimed to determine the durability and strength of an old concrete built-in arch bridge based on selected mechanical, physical, and chemical properties of the concrete. The bridge was erected in 1925 and is located in Jagodnik (northern Poland). Cylindrical specimens were taken from the side ribs connected to the top plate using a concrete core borehole diamond drill machine. The properties of the old concrete were...
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A 95-Year-Old Concrete Arch Bridge: From Materials Characterization to Structural Analysis
PublicationThe structural analysis of a 95-year-old concrete arch bridge located in Jagodnik (Poland) is performed in this paper, in order to check its behavior under today’s traffic loads. The mechanical properties of both the concrete and the reinforcement are investigated by testing cores and bar stubs extracted from the bridge. Structural analysis confirms that the bridge meets today’s load requirements in terms of bearing capacity, serviceability...
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SEM images of dewetted gold films
Open Research DataGold nanostructures were prepared on silicon - Si(111) as a substrate. as a result of dewetting process. Thin golds films were deposited using a table-top dc magnetron sputtering coater under pure Ar plasma conditions . The Au target had 99.99% purity, the rate of Au layer deposition was about 0.4 nm·s−1 and the incident power was 32 W. The thickness...
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Plasmon resonance in gold-silver nanoalloys
Open Research DataSurface plasmon resonance (SPR) can lead to improve or formation a new linear or nonlinear optical phenomena. Especially it can enhance a light emission from luminescence materials. The presence of metal nanostructures or nanoparticles is necessary to excitation of the SPR. It is well known that gold and silver nanostructures exhibit plasmon resonance...
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Spectrofluorometrical Determination of Vitamin B 1 in Different Matrices Using Box‐Behnken Designed Pipette Tip Solid Phase Extraction by a Carbon Nanotube Sorbent
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Application of molecularly imprinted polymer pipette tip micro-solid phase extraction of nalidixic acid and acetaminophen from pills and seawater samples and their determination by spectrophotometry
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Fast determination of bisphenol A in spiked juice and drinking water samples by pipette tip solid phase extraction using cobalt metal organic framework as sorbent
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Application of response surface methodology for silver nanoparticle stir bar sorptive extraction of heavy metals from drinking water samples: a Box–Behnken design
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DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS IN SOIL SAMPLES USING ULTRASONIC PROBE AND SALT-ASSISTED LIQUID-LIQUID EXTRACTION COUPLED WITH HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
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Application of response surface methodology to optimize pipette tip micro-solid phase extraction of dyes from seawater by molecularly imprinted polymer and their determination by HPLC
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Fabrication of a Molecularly Imprinted Polymer Adsorbent for Pipette Tip Micro Solid-phase Extraction of Levofloxacin from Aqueous Samples Prior to its Spectrophotometric Determination
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The comparison of the removal of copper(II) and zinc(II) ions from aqueous solution using 2,6-diaminopyridine in a polymer inclusion membrane and in a classic solvent extraction
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Optimisation of pressurised liquid extraction for elimination of sulphur interferences during determination of organotin compounds in sulphur-rich sediments by gas chromatography with flame photometric detection
PublicationOpracowano metodę analizy osadów dennych na zawartość związków cynoorganicznych (MBT, DBT, TBT, MPhT, DPhT, TPhT) przy użyciu przyspieszonej ekstrakcji rozpuszczalnikiem i chromatografii gazowej z detektorem płomieniowo-fotometrycznym. Detektor ten pozwala selektywnie wykrywać niewielkie ilości pochodnych cynoorganicznych. Niestety, bardzo często spotykana w osadach siarka oraz jej pochodne zakłócają sygnał detektora. Z tego względu...
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Determination of pharmaceutical residues in drinking water in Poland using a new SPE-GC-MS(SIM) method based on Speedisk extraction disks and DIMETRIS derivatization
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Determination of phenylbutazone, sulfamethazine, carbendazim and linuron using a novel pine bark biosorbent for solid-phase extraction (SPE) with high-performance liquid chromatography (HPLC)
PublicationThe paper presents a novel pine bark based biosorbent for solid-phase extraction (SPE). The morphology and composition of the pine bark were characterized using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The biosorbent was used for the preparation of wastewater for the determination of phenylbutazone, sulfamethazine, carbendazim, and linuron by high performance liquid chromatography with...
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Natural deep eutectic solvent based ultrasound assisted liquid-liquid micro-extraction method for methyl violet dye determination in contaminated river water
PublicationSimple and green natural deep eutectic solvent (NADES) based ultrasound assisted liquid-liquid micro-extraction (UA-LLME) method was developed for extraction and determination of methyl violet dye in contaminated river water samples using UV/Vis spectrophotometry. Choline chloride - decanoic acid based natural deep eutectic solvent was used for extraction. Important analytical parameters like pH, deep eutectic solvent volume/phase...
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The Influence of Cold Atmospheric Pressure Plasma-Treated Media on the Cell Viability, Motility, and Induction of Apoptosis in Human Non-Metastatic (MCF7) and Metastatic (MDA-MB-231) Breast Cancer Cell Lines
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Multimodal analysis of traction forces and the temperature dynamics of living cells with a diamond-embedded substrate
PublicationCells and tissues are constantly exposed to chemical and physical signals that regulate physiological and pathological processes. This study explores the integration of two biophysical methods: traction force microscopy (TFM) and optically detected magnetic resonance (ODMR) to concurrently assess cellular traction forces and the local relative temperature. We present a novel elastic substrate with embedded nitrogen-vacancy microdiamonds...
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Jacek Tomków dr hab. inż.
PeopleEducation:1. 2018 - Ph.D. of Technical Sciencesin field of Materials Engineeringthesis: Influence of underwater welding conditions on cold cracking of high strength low alloy steel 2. 2012 - Master of Science Engineer, Gdansk University of Technologyin the field of: Mechanical Engineeringwith specialization in: Manufacturing Engineering and Computer Aided Manufacturing Processesthesis: Non-destructive testing of welding joints...
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Escherichia coli lipopolysaccharide may affect the endothelial barrier and IL‐10 expression of apolipoprotein B100‐pulsed dendritic cells
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The LD loop as an important structural element required for transmission of the allosteric signal in the HtrA (DegP) protease from Escherichia coli
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How the toxicity of nanomaterials towards different species could be simultaneously evaluated: a novel multi-nano-read-across approach
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Aktywność amylolityczna ciałek inkluzyjnych izolowanych z Escherichia coli BL21(DE3)pLysS transformowanej plazmidem pTYB2alfa-Amyl.
PublicationBadania ekspresji rekombinantowej alfa-amylazy izolowanej z Escherichia coli BL21(DE3)pLysS transformowanej plazmidem pTYB2alfa-Amyl wykazały, że około 49 % wytwarzanego enzymu występuje w postaci ciałek inkluzyjnych. Źródłem genu termostabilnej alfa-amylazy był szczep Pyrococcus woesei (DSM 3773). W celu uzyskania aktywnych białek z ciałek inkluzyjnych odwirowane komórki bakterii inkubowano w roztworze CaCl2 i po trawieniu lizozymem...
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Letter to the Editor: Complete resonance assignments of the 'donor-strand' AfaD: The afimbrial invasin from Diffusely Adherent E. coli.
PublicationUstalono strukturę NMR rekombiantowego białka AfaD-dsc. Uzyskane wyniki są podstawą do badań rentgenograficznych struktury białek AfaD/DraD.
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Complementation studies of the DnaK–DnaJ–GrpE chaperone machineries from Vibrio harveyi and Escherichia coli, both in vivo and in vitro
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Functional Similarities and Differences of an Archaeal Hsp70(DnaK) Stress Protein Compared with its Homologue from the Bacterium Escherichia coli
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The impact of training and neurotrophins on functional recovery after spinal cord transection: cellular and molecular mechanisms contributing to motor improvement
PublicationBeneficial effects of locomotor training on the functional recovery after complete transection of the spinal cord indicate that in chronic spinal animals spontaneous recovery processes are enhanced and shaped by the training. The mechanisms of that use-dependent improvement are still not fully understood. This review tackles three aspects of this issue: (1) neurochemical attributes of functional improvement...
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Phospholipid-functionalized gold electrode for cellular membrane interface studies - interactions between DMPC bilayer and human cystatin C
PublicationThis work describes the electrochemical studies on the interactions between V57G mutant of human cystatin C (hCC V57G) and membrane bilayer immobilized on the surface of a gold electrode. The electrode was modified with 6-mercaptohexan-1-ol (MCH) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). DMPC was used as a membrane mimetic for monitoring electrochemical changes resulting from the interactions between the functionalized...
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Wykorzystanie komórek Escherichia coli transformowanych genem termostabilnej beta-galaktozydazy z Pyrococcus woesei do wytwarzania galaktozylofruktozy
PublicationKomórki rekombinantowej Escherichia coli transformowane genem beta-galaktozydazy z Pyrococcus woesei wykorzystano jako biokatalizator syntezy galaktozylofruktozy. Największą aktywność syntezy wymienionego prebiotyku (7,6 g/L x godz)uzyskano w 80C przy pH 5,4, stosując jako substrat roztwór zawierający 10% laktozy i 20% fruktozy. Aktywność transgalaktozylacji nie ulegała istotnym zmianom podczas sześciu kolejnych szarż produkcyjnych.
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Excess production of phage λ delayed early proteins under conditions supporting high Escherichia coli growth rates
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Molecular and structural basis of inner core lipopolysaccharide alterations in Escherichia coli: incorporation of glucuronic acid and phosphoethanolamine in the heptose region.
PublicationIt is well established that lipopolysaccharide (LPS) often carries nonstoichiometric substitutions in lipid A and in the inner core. In this work, the molecular basis of inner core alterations and their physiological significance are addressed. A new inner core modification of LPS is described, which arises due to the addition of glucuronic acid on the third heptose with a concomitant loss of phosphate on the second heptose. This...
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Identification of antigen Ag43 in uropathogenic Escherichia coli Dr+ strains and defining its role in the pathogenesis of urinary tract infections
PublicationUrinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) are among the most common bacterial infectious diseases in the developed world. The urovirulence of UPEC is mainly associated with the surface-exposed fimbrial adhesins and adhesins of the autotransporter (AT) family. The best studied of theses proteins is antigen 43 (Ag43) mediating cell aggregation, adhesion and biofilm development as the causes of...
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Synthesis and transport studies of model dipeptides with modified n-terminal amino groups into e. coli k12 mutant strains
Publicationotrzymano na drodze syntezy chemicznej kilka modelowych dipeptydów zawierających n-terminalna guanidynę oraz betainę. zbadano transport tych peptydów do komorek e. coli k12, posiadających zróżnicowane systemy transportowe. wyniki badań potwierdziły brak transportu do komórek bakteryjnych z wykorzystaniem permeaz peptydowych. dodatnio naładowana i silnie polarna grupa aminowa uniemożliwia efektywny transport tych związków do komórek...
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Antibiotic resistance, virulence, and phylogenetic analysis of Escherichia coli strains isolated from free-living birds in human habitats
PublicationWild birds can be colonized by bacteria, which are often resistant to antibiotics and have various virulence profiles. The aim of this study was to analyze antibiotic resistance mechanisms and virulence profiles in relation to the phylogenetic group of E. coli strains that were isolated from the GI tract of wildfowl. Out of 241 faecal samples, presence of E. coli resistant to a cephalosporin (ESBL/AmpC) was estimated for 33 isolates...
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Construction, purification, and functional characterization of His-tagged Candida albicans glucosamine-6-phosphate synthase expressed in Escherichia coli
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