Wyniki wyszukiwania dla: CLINICAL SAMPLES
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Proximal fibular osteotomy as a treatment for degenerative meniscal extrusion
PublikacjaMeniscal extrusion treatment is a key focus area for research and clinical study of degenerative knee pathology. The contact forces between the meniscus and the bones cause external displacements of the meniscus that are resisted by the circumferential fibres. The main risk factors for the excessive deformation of the meniscus are meniscal root tears, disruption of the circumferential fibres, knee malalignment and high body mass...
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Evaluation of possibilities of genotyping of Candida glabrata clinical isolates with RAPD-PCR method and microsatellite analysis
PublikacjaThe aim of the study was to compare the discriminatory power of RAPD-PCR method using RSD10 primer and microsatellite (GACA)4 analysis for genotyping clinical isolates of C. glabrata. Isolates were received from patients of two Polish hospitals: Children's Memorial Health Institute in Warsaw (n=17) and Medical University of Gdansk (n=37). Species identification was confirmed by two phenotypic methods: growth on chromagar plates...
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Metabolic transformation of antitumor acridinone C-1305 but not C-1311 via selective cellular expression of UGT1A10 increases cytotoxic response: implications for clinical use.
PublikacjaThe acridinone derivates C-1305 and C-1311 are promising antitumor agents with high activity against several experimental cellular and tumor models and which are under evaluation in pre-clinical and early phase clinical trials. Recent evidence from our laboratories has indicated that both compounds were conjugated by several UGT isoforms with the most active being extrahepatic UGT1A10. The present studies were designed to test...
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Exploring the factor structure and the validity of the abbreviated Basic and Earning Self-Esteem Scales
PublikacjaThe original longer versions (Forsman & Johnson, 1996) and abbreviated versions of the Basic and Earning Self-Esteem Scales have been used in clinical and non-clinical settings, but little is known about the factor structure and the validity of these scales in their abbreviated forms. The original longer versions of the scales comprise several dimensions, but both abbreviated versions of the scales have been interpreted as if they...
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Therapeutic Potential of Multifunctional Tacrine Analogues
PublikacjaTacrine is a potent inhibitor of cholinesterases (acetylcholinesterase and butyrylcholinesterase) that shows limiting clinical application by liver toxicity. In spite of this, analogues of tacrine are considered as a model inhibitor of cholinesterases in the therapy of Alzheimer’s disease. The interest in these compounds is mainly related to a high variety of their structure and biological properties. In the present review, we...
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Current situation in Polish nuclear medicine and Polish example of model Nuclear Medicine Centre meeting European Union requirements
PublikacjaNuclear medicine is currently a well-established part of medicine. It is applied in many fields of clinical medicine and science like endocrinology, oncology, cardiology, molecular medicine and engineering, radiopharmacy, physics and information science. Due to its increasing importance and application, several regulation and supervision bodies have been founded to ensure safe usage of radiation and to improve diagnostic and therapeutic...
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Amygdalin: Toxicity, Anticancer Activity and Analytical Procedures for Its Determination in Plant Seeds
PublikacjaAmygdalin (D-Mandelonitrile 6-O--D-glucosido--D-glucoside) is a natural cyanogenic glycoside occurring in the seeds of some edible plants, such as bitter almonds and peaches. It is a medically interesting but controversial compound as it has anticancer activity on one hand and can be toxic via enzymatic degradation and production of hydrogen cyanide on the other hand. Despite numerous contributions on cancer cell lines, the clinical...
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Recent developmentsinthesynthesisand biological activityofacridine/acridoneanalogues
PublikacjaMany people in the world struggle with cancer or bacterial, parasitic, viral, Alzheimer's and other diseases. Therefore, many scientists seek new, more effective, more selective and less toxic drugs. Acridine/acridone derivatives constitute a class of compounds with a broad spectrum of biological activity and are of great interest to scientists. Todate, many acridine/acridone analogues have been obtained,which, inter alia, exhibit...
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New Peptide Based Fluconazole Conjugates with Expanded Molecular Targets
PublikacjaInfections of Candida spp. etiology are frequently treated with azole drugs. Among azoles, the most widely used in the clinical scenario remains fluconazole (FLC). Promising results in treatment of dangerous, systemic Candida infections demonstrate the advantages of combined therapies carried out with combinations of at least two different antifungal agents. Here, we report five conjugates composed of covalently linked FLC and...
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Sample preparation and recent trends in volatolomics for diagnosing gastrointestinal diseases
PublikacjaThe analysis of the human volatilome can be successfully used for rapid and non-invasive diagnostics of gastrointestinal diseases. However, the introduction of techniques based on detection of volatiles is limited, among other factors, by difficulties which arise during the sampling stage and instrumental analysis. The aim of this article was to review and discuss medical and analytical literature on the analysis of volatiles in...
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Active Dynamic Thermography in Medical Diagnostics
PublikacjaThis is an overview of active thermal imaging methods in medical diagnostics using external thermal stimulation. In this chapter, several clinical cases diagnosed using the active dynamic thermography method, ADT, are presented. Features of this technology are discussed and main advantages underlined. Applications in skin burn diagnostics and quantitative evaluation leading to modern classification of burned patients for further...
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Rapid Assays for Specific Detection of Fungi of Scopulariopsis and Microascus Genera and Scopulariopsis brevicaulis Species
PublikacjaPurpose Fungi of Scopulariopsis and Microascus genera cause a wide range of infections, with S. brevicaulis being the most prevalent aetiological agent of mould onychomycosis. Proper identification of these pathogens requires sporulating culture, which considerably delays the diagnosis. So far, sequencing of rDNA regions of clinical isolates has produced ambiguous results due to the lack of reference sequences in publicly available...
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Numerical and Clinical Analysis of an Eyeball Injuries under Direc Impact
PublikacjaObjectives: The objective of this study was to develop a numerical model of the eyeball and orbit to simulate a blunt injury to the eyeball leading to its rupture, as well as to conduct a comparative analysis of the results obtained using the finite element method against the clinical material concerning patients who had suffered an eyeball rupture due to a blunt force trauma. Material and Methods: Using available sclera biometric and...
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Ultrasound and Clinical Preoperative Characteristics for Discrimination Between Ovarian Metastatic Colorectal Cancer and Primary Ovarian Cancer: A Case-Control Study
PublikacjaThe aim of this study was to describe the clinical and sonographic features of ovarian metastases originating from colorectal cancer (mCRC), and to discriminate mCRC from primary ovarian cancer (OC). We conducted a multi-institutional, retrospective study of consecutive patients with ovarian mCRC who had undergone ultrasound examination using the International Ovarian Tumor Analysis (IOTA) terminology, with the addition of evaluating...
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Host and pathogen factors in Klebsiella pneumoniae upper urinary tract infections in renal transplant patients
PublikacjaPurpose . To analyse the role of virulence factors (VFs) and host in Klebsiella pneumoniae upper urinary tract infections (UTIs) in renal transplant (RTx) recipients. Methodology. Clinical and demographic data were registered prospectively. Phylogenetic background of K. pneumoniae isolates was analysed by PCR melting profiles (MP) and the following VFs genes: fimH-1, uge, kpn, ycfM, mrkD, rmpA, magA, hlyA, cnf-1, irp-1, irp-2,...
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Sensors for Rapid Detection of Environmental Toxicity in Blood of Poisoned People
PublikacjaRecently, the diagnosis and treatment of poisoned person can be done only in specialized centers. Furthermore, currently used clinical methods of intoxication diagnosis are not sufficient for early detection. Conventional laboratory tests based on urine and blood require professional, high skilled staff, high cost equipment as well as they are arduous and lasting analytical procedures. There is a need to elaborate relatively cheap...
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5-Bromo-2′-deoxycytidine—a potential DNA photosensitizer
PublikacjaA double-stranded oligonucleotide, 80 base pairs in length, was multiply labeled with 5-bromo-2′-deoxycytidine (BrdC) using polymerase chain reaction (PCR). The modified oligonucleotide was irradiated with 300 nm photons and its damage was assayed by employing DHPLC, LC-MS and denaturing polyacrylamide gel electrophoresis (PAGE). Two types of damage were demonstrated, namely, single strand breaks (SSBs) and intrastrand cross-links...
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Application of vibrational spectroscopy, thermal analyses and X-Ray diffraction in the rapid evaluation of the stability in solid-state of ranitidine, famotidine and cimetidine
PublikacjaThis paper reports the study on applicability of Fourier transform infrared (FTIR), near-infrared (NIR) and Raman spectroscopy, differential scanning calorimetry (DSC) and X-ray diffraction (XRD) for the estimation of the chemical stability and photostability of histamine H2-receptor antagonist substances. Ranitidine hydrochloride (RAN), famotidine (FAM) and cimetidine (CIM) were tested and differences in sensitivity were measured...
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Genotyping of clinical isolates of fluconazole-resistant Candida albicans
PublikacjaThe aim of the study was to compare the discriminatory power of RAPD-PCR method with using RSD10 primer and microsatellite analysis with using (GACA)4 primer for genotyping clinical isolates of fluconazole-resistant C. albicans. Isolates were received from patients of Children's Memorial Health Institute in Warsaw. However in the case of both tested methods low number of amplified fragments was generated, nine and six different...
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Use of optical skin phantoms for preclinical evaluation of laser efficiency for skin lesion therapy
PublikacjaSkin lesions are commonly treated using laser heating. However, the introduction of new devices into clinical practice requires evaluation of their performance. This study presents the application of optical phantoms for assessment of a newly developed 975-nm pulsed diode laser system for dermatological purposes. Such phantoms closely mimic the absorption and scattering of real human skin (although not precisely in relation to...
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BLOOD PRESSURE ESTIMATION BY MEANS OF A JOINT IMPEDANCE– PHOTOPLETYSMOGRAPHIC METHOD
PublikacjaThe knowledge of patient’s day to day blood pressure changes is invaluable to physicians for both diagnostics and health monitoring reasons. Constant observation of the pressure throughout a day would provide even more valuable clinical information. A convenient non-invasive methods of blood pressure estimation for monitoring purposes are widely proposed. This work shows a statistical approach to...
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Conducting polymers for biodegradable metallic implants
PublikacjaNowadays permanent metallic cardiovascular stents are long-term implants. The long-time presence of such an implant in human body can cause overgrowth of tissue within the treated portion of the vessel, blockage of the circulatory system and many other clinical complications, such as thrombosis, prolonged physical irritations or chronic inflammation. Therefore, in recent years, there is an interest to create biodegradable metallic...
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Transcriptional profile of in vitro expanded human epidermal progenitor cells for the treatment of non-healing wounds
PublikacjaBackground Epidermal progenitor cells (EPCs) have been under extensive investigation due to their increasing potential of application in medicine and biotechnology. Cultured human EPCs are used in the treatment of chronic wounds and have recently became a target for gene therapy and toxicological studies. One of the challenges in EPCs culture is to provide a high number of undifferentiated, progenitor cells displaying high viability...
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The Role of Inflammatory Cytokines in the Pathogenesis of Colorectal Carcinoma-Recent Findings and Review
PublikacjaThe inflammatory process plays a significant role in the development of colon cancer (CRC). Intestinal cytokine networks are critical mediators of tissue homeostasis and inflammation but also impact carcinogenesis at all stages of the disease. Recent studies suggest that inflammation is of greater importance in the serrated pathway than in the adenoma-carcinoma pathway. Interleukins have gained the most attention due to their...
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The biological role of prolyl oligopeptidase and the procognitive potential of its peptidic inhibitors from food proteins
PublikacjaProlyl oligopeptidase (POP) is a conserved serine protease belonging to proline-specific peptidases. It has both enzymatic and non-enzymatic activity and is involved in numerous biological processes in the human body, playing a role in e.g., cellular growth and differentiation, inflammation, as well as the development of some neurodegenerative and neuropsychiatric disorders. This article describes the physiological and pathological...
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A new diagnostic method for evaluation of cardiosurgery wound healing
PublikacjaOne of important and still unsolved problems in medicine is the question of objective and quantitative evaluation of post-surgery wound healing. The aim of this research is to analyse possibility and value of using the newly elaborated infrared imaging procedure – Active Dynamic Thermography (ADT) – for quantitative description of wound healing processes in cardiosurgery. Both, classical thermal figures of merit as well as ADT...
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Biomedical engineering of polysaccharide-based tissue adhesives: Recent advances and future direction
PublikacjaTissue adhesives have been widely used for preventing wound leaks, sever bleeding, as well as for enhancing drug delivery and biosensing. However, only a few among suggested platforms cover the circumstances required for high-adhesion strength and biocompatibility, without toxicity. Antibacterial properties, controllable degradation, encapsulation capacity, detectability by image-guided procedures and affordable price are also...
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HDAC Inhibitors: Innovative Strategies for Their Design and Applications
PublikacjaHistone deacetylases (HDACs) are a large family of epigenetic metalloenzymes that are involved in gene transcription and regulation, cell proliferation, differentiation, migration, and death, as well as angiogenesis. Particularly, disorders of the HDACs expression are linked to the development of many types of cancer and neurodegenerative diseases, making them interesting molecular targets for the design of new efficient drugs...
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Amino Acid and Peptide‐Based Antiviral Agents
PublikacjaA significant number of antiviral agents used in clinical practice are amino acids, short peptides, or peptidomimetics. Among them, several HIV protease inhibitors (e. g. lopinavir, atazanavir), HCV protease inhibitors (e. g. grazoprevir, glecaprevir), and HCV NS5A protein inhibitors have contributed to a significant decrease in mortality from AIDS and hepatitis. However, there is an ongoing need for the discovery of new antiviral...
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Cancer immune escape: the role of antigen presentation machinery
PublikacjaThe mechanisms of antigen processing and presentation play a crucial role in the recognition and targeting of cancer cells by the immune system. Cancer cells can evade the immune system by downregulating or losing the expression of the proteins recognized by the immune cells as antigens, creating an immunosuppressive microenvironment, and altering their ability to process and present antigens. This review focuses on the mechanisms...
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ADT in mammography
PublikacjaWe discuss limitations of the known methods of IR imaging in diagnostics of breast cancer. In conclusion we show that for practical reasons one requires new approaches because the known methods based on simple observation of external temperature distribution are not fully effective. Even advanced pattern recognition could not help too much for static images. We ask the question: may active dynamic thermography, known in nondestructive...
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Tools for Automatic Wound Healing Evaluation
PublikacjaFinal results of the research project devoted to development of a new objective and quantitative method of post cardiosurgery wound healing diagnostics, already preliminary discussed at the QIRT 2014 Conference, is presented. We assumed that thermal processes should be effective in subtle description of temporal changes of tissue thermal properties after surgical interventions. To prove this assumption more than 400 patients after...
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Phosphoroorganic Metal Complexes in Therapeutics
PublikacjaThe present mini-review highlights recent developments on antitumor activity of metal-based therapeutics which have been a subject of researches for the last few decades. In 1965, Rosenberg found that during an electrolysis on platinum electrodes a complex of Pt is generated which inhibited to a great extent a binary fission in Escherichia coli bacteria. This discovery started a new chapter in medicinal chemistry and the interesting...
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Development and validation of a model that includes two ultrasound parameters and the plasma D-dimer level for predicting malignancy in adnexal masses: an observational study
PublikacjaBackground: Pre-operative discrimination of malignant from benign adnexal masses is crucial for planning additional imaging, preparation, surgery and postoperative care. This study aimed to define key ultrasound and clinical variables and develop a predictive model for calculating preoperative ovarian tumor malignancy risk in a gynecologic oncology referral center. We compared our model to a subjective ultrasound assessment (SUA)...
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Cannabinoids: Challenges, opportunities and current techniques towards its extraction and purification for edibles
PublikacjaDue to the multiple health benefits associated with the consumption of cannabinoids, there is a current trend on continuing to explore their biological properties. Cannabidiol (CBD) is likely to be the most investigated endocannabinoid from the cannabis plant. Unfortunately, the purification of CBD from its natural source and psychotropic analogue (tetrahydrocannabinol, THC) is timely challenging for the research community based...
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Low-Molecular Pyrazine-Based DNA Binders: Physicochemical and Antimicrobial Properties
PublikacjaPyrazine and its derivatives are a large group of compounds that exhibit broad biological activity, the changes of which can be easily detected by a substituent effect or a change in the functional group. The present studies combined theoretical research with the density functional theory (DFT) approach (B3LYP/6-311+G**) and experimental (potentiometric and spectrophotometric) analysis for a thorough understanding of the structure...
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Modelling of spirometry - the application for diagnostic purposes
PublikacjaThe process of the tissue oxygen supply is the fundamental process of the human life. The lung functioning consists of three processes: the ventilation, the perfusion and the diffusion. The paper deals with one of these processes which is the ventilation process. The quality of the pulmonary ventilation has a strong influence on the patient's health condition. Numerous limitations are imposed on conditions of the lung quality measurement,...
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Metal nanoparticles-assisted early diagnosis of diseases
PublikacjaEarly diagnosis is essential for the effective illness treatment, but traditional diagnostic approaches inevitably have major downsides. Recent advancements in nanoparticle-based biosensors have created new opportunities for accelerating diagnosis. High surface area, exceptional sensitivity, high specificity, and optical characteristics of metal and metal oxide nanoparticles have made it possible to detect a variety of health conditions...
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Unsupervised machine-learning classification of electrophysiologically active electrodes during human cognitive task performance
PublikacjaIdentification of active electrodes that record task-relevant neurophysiological activity is needed for clinical and industrial applications as well as for investigating brain functions. We developed an unsupervised, fully automated approach to classify active electrodes showing event-related intracranial EEG (iEEG) responses from 115 patients performing a free recall verbal memory task. Our approach employed new interpretable...
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Usefulness of chest perfusion computed tomography in the diagnosis of diabetic pulmonary microangiopathy
PublikacjaThis paper presents the usefulness of perfusion computed tomography (pCT) in the diagnosis of diabetic pulmonary microangiopathy. Our previous works have shown that perfusion parameters are useful in the diagnosis of diabetic pulmonary microangiopathy. We are looking for such measurements and perfusion parameters that provide the most accurate diagnosis. Two types of comparison were made based on the results of clinical trials:...
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Active Dynamic Infrared Thermal Imaging in Burn Depth Evaluation
PublikacjaThe aim of this study was to find the relationship between active dynamic thermography (ADT) with cold excitation and burn depth. This new modality of evaluation of burns seems to be an attractive proposal for quantitative classification, allowing proper choice of burn wound treatment: conservative or surgical, especially compared with static thermography. The work was an in vivo experiment on domestic pigs, and a small number...
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Consciousness Study of Subjects with Unresponsive Wakefulness Syndrome Employing Multimodal Interfaces
PublikacjaThe paper presents a novel multimodal-based methodology for consciousness study of individuals with unresponsive wakefulness syndrome. Two interfaces were employed in the experiments: eye gaze tracking system – CyberEye developed at the Multimedia Systems Department, and EEG device with electrode placement in the international 10-20 standard. It was a pilot study for checking if it is possible to determine objective methods based...
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Molecular Targets for Anticandidal Chemotherapy
PublikacjaA relatively small number of anticandidal chemotherapeutics used in clinical practice is at least in part consequence of a limited number of their molecular targets: ergosterol in the membrane, lanosterol demethylase, b(1!3) glucan synthase, and DNA/RNA biosynthesis. Much more potential novel targets have been revealed by the comparative genomic studies identifying essential genes unique for Candida albicans or resulted from recognition...
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Comprehensive Review of Fluorescence Applications in Gynecology
PublikacjaSince the introduction of indocyanine green (ICG) as a fluorophore in near-infrared imaging, fluorescence visualization has become an essential tool in many fields of surgery. In the field of gynecology, recent new applications have been proposed and found their place in clinical practice. Different applications in gynecology were investigated, subcategorized, and overviewed concerning surgical applications and available dyes....
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Mapping the Structural and Dynamic Determinants of pH-Sensitive Heparin Binding to Granulocyte Macrophage Colony Stimulating Factor
PublikacjaGranulocyte macrophage colony stimulating factor (GMCSF) is an immunomodulatory cytokine that is harnessed as a therapeutic. GMCSF is known to interact with other clinically important molecules, such as heparin, suggesting that endogenous and administered GMCSF has the potential to modulate orthogonal treatment outcomes. Thus, molecular level characterization of GMCSF and its interactions with biologically active compounds is critical...
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Natural carbon-based quantum dots and their applications in drug delivery: A review
PublikacjaNatural carbon based quantum dots (NCDs) are an emerging class of nanomaterials in the carbon family. NCDs have gained immense acclamation among researchers because of their abundance, eco-friendly nature, aqueous solubility, the diverse functionality and biocompatibility when compared to other conventional carbon quantum dots (CDs).The presence of different functional groups on the surface of NCDs such as thiol, carboxyl, hydroxyl,...
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Information Extraction from Polish Radiology Reports using Language Models
PublikacjaRadiology reports are vital elements of directing patient care. They are usually delivered in free text form, which makes them prone to errors, such as omission in reporting radiological findings and using difficult-to-comprehend mental shortcuts. Although structured reporting is the recommended method, its adoption continues to be limited. Radiologists find structured reports too limiting and burdensome. In this paper, we propose...
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Phage Therapy—Challenges, Opportunities and Future Prospects
PublikacjaThe increasing drug resistance of bacteria to commonly used antibiotics creates the need to search for and develop alternative forms of treatment. Phage therapy fits this trend perfectly. Phages that selectively infect and kill bacteria are often the only life-saving therapeutic option. Full legalization of this treatment method could help solve the problem of multidrug-resistant infectious diseases on a global scale. The aim of...
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Nanoparticle Tracking Analysis of Urinary Extracellular Vesicle Proteins as a New Challenge in Laboratory Medicine
PublikacjaUrinary extracellular vesicle (uEV) proteins may be used as specific markers of kidney damage in various pathophysiological conditions. The nanoparticle-tracking analysis (NTA) appears to be the most useful method for the analysis of uEVs due to its ability to analyze particles below 300 nm. The NTA method has been used to measure the size and concentration of uEVs and also allows for a deeper analysis of uEVs based on their protein...
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IR-THERMAL IMAGING IN CARDIOSURGERY
PublikacjaA method for monitoring the state of the myocardium during cardiosurgical interventions based on thermal IR imaging is presented. These methods called Static Thermography and Active Dynamic Thermography (ADT) uses information about distribution of temperature on the surface and an external excitation source to induce thermal transient processes in a tested object. Recording time series of thermograms allows to calculate parametric...