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Borrelia burgdorferi BmpA-BBK32 and BmpA-BBA64: New Recombinant Chimeric Proteins with Potential Diagnostic Value
PublikacjaCurrently, the diagnosis of Lyme disease is based mostly on two-tiered serologic testing. In the new generation of immunoenzymatic assays, antigens comprise whole-cell lysates of members of the Borrelia burgdorferi sensu lato (s.l.) species complex, with the addition of selected recombinant proteins. Due to the high diversity of members of the B. burgdorferi s.l. genospecies and the low degree of conservation among the amino acid...
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Adsorption onto zeolites: molecular perspective
Publikacja2D minerals are among key elements of advanced systems, but the need for understanding their interactions/reactions with materials and systems in which they are involved necessitates tracking their molecular and atomic monitoring. Zeolitic structures are microporous materials formed in the nature through volcanic activities or synthesis. Because of their outstanding physicochemical properties like cation exchange capacity and excellent...
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Evaluation of the glycerolysis process and valorisation of recovered polyol in polyurethane synthesis
PublikacjaIn this study, the glycerolysis of polyurethane (PU) foam and the applicability of obtained glycerolysate (GLY) for cast PUs were investigated. It was found that crude glycerine with purity grade of 84% might be successfully used for the glycerolysis. The optimal conditions were determined as follows: reaction time of 60 min at 220 °C using dibutyltin dilaurate catalyst (0.5%). Moreover, the purification of GLY by means of liquid-liquid...
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Homogeneity study of candidate reference material (contaminated soil) based on determination of selected metals, PCBs and PAHs
PublikacjaReference materials (RMs) are an indispensable part of QA/QC system and have played a critical role in the continual expansion of analytical capabilities of the analytical laboratories. The homogeneity study of the candidate for reference material is essential for the quality of reference material (certified reference material) used in quality assurance activities. The homogeneity study of candidate for reference materials of contaminated...
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UV-induced electron transfer between triethylamine and 5-bromo-2′-deoxyuridine. A puzzle concerning the photochemical debromination of labeled DNA
Publikacja5-Bromo-2'-deoxyuridine (BrdU) photosensitizes DNA to strand break formation. However, this type of photodamage is completely quenched by the presence of triethylamine (TEA) which originates from RP-HPLC purification commonly employed by oligonucleotide providers. While the presence of TEA in oligonucleotide samples does not interfere with PCR or other molecular biology applications, the mechanism of photochemical reaction proceeding...
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Size Exclusion Coupled to Reversed Phase Liquid Chromatography for the Characterization of Cranberry Products
PublikacjaA combination of solvent extraction, size exclusion purification, and reversed-phase liquid chromatography with UV absorption and fluorescence detection was here used for the determination of flavan-3-ols and related oligomeric compounds in cranberry products. The method relied on robust, simple, and inexpensive instrumental equipment present in most of routine analytical laboratories. Sample extracts in methanol/water/hydrochloric...
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Wieloobszarowa rozmyta regulacja PID mocy reaktora jądrowego
PublikacjaW artykule przedstawiono wieloobszarowy regulator rozmyty z lokalnymi regulatorami PID dla sterowania mocą reaktora jądrowego typu PWR. Wykorzystano model matematyczny o parametrach skupionych reaktora PWR obejmujący procesy generacji i wymiany ciepła oraz efektów reaktywnościowych. Nastawy lokalnych regulatorów PID zostały dobrane w sposób optymalny, minimalizując całkowy wskaźnik jakości ISE. Na przykładzie pokazano że zastosowane...
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Wpływ wysokiej osteotomii piszczelowej na mechanikę stawu kolanowego
PublikacjaW rozprawie podjęto próbę weryfikacji wpływu zabiegu chirurgicznego wysokiej osteotomii piszczelowej na mechanikę stawu kolanowego. Po wykonanym przeglądzie literatury, postawiono trzy hipotezy, które poddano weryfikacji na drodze badań numerycznych. Otrzymane wyniki zwalidowano poprzez badania nacisków powierzchniowych występujących w stawach kolanowych świni domowej, prowadzonych na maszynie do jednoosiowego ściskania. Praca...
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Ekonometryczna analiza konsumpcji w Polsce w latach 1995-2018- statyczne modele wydatków konsumpcyjnych w wartościach nominalnych
PublikacjaW artykule przeprowadzono badania makroekonomicznych związków pomiędzy konsumpcją globalną (C) a produktem krajowym brutto (PKB). W części teoretycznej artykułu: • zdefiniowano postacie analityczne przyczynowo-skutkowych makromodeli konsumpcji, • sformułowano interpretację mierników opisujących badane zależności, • sformułowano założenia stochastyczne dla rozważanych makromodeli konsumpcji, W części empirycznej artykułu:...
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Zarządzanie terminowością w projektach. Wskaźniki i narzędzia
PublikacjaCzas jest istotnym elementem tzw. trójkąta projektu, dlatego też terminowość, rozu-miana, jako realizacja przedsięwzięcia w zadanym czasie, to parametr, który powinien podle-gać szczególnemu nadzorowi, a ryzyko nieterminowej realizacji powinno być wykrywane niezwłocznie. Istniejące już metody, takie jak np. wartość wypracowana (j.ang Earned Value - EV) pozwalają zaadresować te wyzwania, jednak ze względu na swoją złożoność mogą...
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Spatio-Temporal Validation of GNSS-Derived Global Ionosphere Maps Using 16 Years of Jason Satellites Observations
PublikacjaExisting ionospheric models perform very well in mapping the calm state of the ionosphere. However, the problem is accurately determining the total electron content (TEC) for disturbed days. Knowledge of the exact electron density is essential for single−frequency receivers, which cannot eliminate the ionospheric delay. This study aims to investigate temporal and spatial variability in the distribution of TEC based on differences...
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Machine Learning-Based Wetland Vulnerability Assessment in the Sindh Province Ramsar Site Using Remote Sensing Data
PublikacjaWetlands provide vital ecological and socioeconomic services but face escalating pressures worldwide. This study undertakes an integrated spatiotemporal assessment of the multifaceted vulnerabilities shaping Khinjhir Lake, an ecologically significant wetland ecosystem in Pakistan, using advanced geospatial and machine learning techniques. Multi-temporal optical remote sensing data from 2000 to 2020 was analyzed through spectral...
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Dietary behaviour and physical activity policies in Europe: learnings from the Policy Evaluation Network (PEN)
PublikacjaThe European Policy Evaluation Network (PEN), initiated in autumn 2018, aimed at advancing the evidence base for public policies impacting dietary behaviour, physical activity and sedentary behaviours in Europe. This is needed because non-communicable diseases—the leading cause of global mortality—are substantially caused by physical inactivity and unhealthy dietary behaviours, which in turn are driven by upstream factors that...
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Accelerated Gradient-Based Optimization of Antenna Structures Using Multi-Fidelity Simulations and Convergence-Based Model Management Scheme
PublikacjaThe importance of numerical optimization has been steadily growing in the design of contemporary antenna structures. The primary reason is the increasing complexity of antenna topologies, [ a typically large number of adjustable parameters that have to be simultaneously tuned. Design closure is no longer possible using traditional methods, including theoretical models or supervised parameter sweeping. To ensure reliability, optimization...
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At the Limits of Criticality-Based Quantum Metrology: Apparent Super-Heisenberg Scaling Revisited
PublikacjaWe address the question of whether the super-Heisenberg scaling for quantum estimation is indeed realizable. We unify the results of two approaches. In the first one, the original system is compared with its copy rotated by the parameter-dependent dynamics. If the parameter is coupled to the one-body part of the Hamiltonian, the precision of its estimation is known to scale at most as N−1 (Heisenberg scaling) in terms of the number...
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Gamma oscillations precede interictal epileptiform spikes in the seizure onset zone
PublikacjaOBJECTIVE: To investigate the generation, spectral characteristics, and potential clinical significance of brain activity preceding interictal epileptiform spike discharges (IEDs) recorded with intracranial EEG. METHODS: Seventeen adult patients with drug-resistant temporal lobe epilepsy were implanted with intracranial electrodes as part of their evaluation for epilepsy surgery. IEDs detected on clinical macro- and research microelectrodes...
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THE ART OF VIRTUAL INFORMATION EXCHANGE AND ITS INFLUENCE ON REAL CITY SPACE
PublikacjaThe paper investigates the impact of the new genres of contemporary public art based on the virtual information exchange in real spaces of the cities. On the basis of theoretical approaches, trans-disciplinary surveys and case studies, the paper focuses on the art that not only relies on the technological support but also refers to the new public sphere, where people are co-creators of the art object giving their personal input...
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Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective.
PublikacjaThe RpoE sigma factor is essential for the viability of Escherichia coli. RpoE regulates extracytoplasmic functions including lipopolysaccharide (LPS) translocation and some of its non-stoichiometric modifications. Transcription of the rpoE gene is positively autoregulated by EσE and by unknown mechanisms that control the expression of its distally located promoter(s). Mapping of 5′ ends of rpoE mRNA identified five new transcriptional...
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Heptaene macrolides biosynthesis by Streptomyces species
PublikacjaPolyene macrolides are one of the groups of secondary metabolites, generated by microorganisms belonging to the Streptomyces genus. These compounds, containing 3 - 7 conjugated double bonds systems in their molecules exhibit high antifungal activity against a broad spectrum of fungal pathogens with heptaenes demonstrating the highest antifungal potential. At the large extent, efficiency of biosynthesis of these natural products...
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Thermal and technological aspects of double face grinding of C45 carbon steel
PublikacjaIn grinding, the contact zone temperature is a decisive factor influencing the achievable surface quality and the grinding tool wear. In contrast to other grinding processes, only few information regarding double face grinding with planetary kinematics when processing steel is known up to date. Since the successive substitution of in-dustrial double-sided lapping processes by double-sided grinding, it has become necessary to the...
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Morphology Regulation Mechanism and Enhancement of Photocatalytic Performance of BiOX (X = Cl, Br, I) via Mannitol-Assisted Synthesis
PublikacjaBiOX (X = Cl, Br, I) photocatalysts with dominant (110) facets were synthesized via a mannitol-assisted solvothermal method. This is the first report on the exposed (110) facets-, size-, and defects-controlled synthesis of BiOX achieved by solvothermal synthesis with mannitol. This polyol alcohol acted simultaneously as a solvent, capping agent, and/or soft template. The mannitol concentration on the new photocatalysts morphology...
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Torque capacity of multidisc wet clutch with reference to friction occurrence on its spline connections
PublikacjaIn this article developed mathematical model that includes friction occurrence on spline connections is presented. The work also contains results of experimental research on torque capacity of multidisc wet clutch. These results are expressed as a function of contact pressure for different number of friction surfaces. Due to increased interest in research concerning multidisc wet clutches it is essential to determine impact of...
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Curing epoxy with ethylenediaminetetraacetic acid (EDTA) surface-functionalized Co Fe3-O4 magnetic nanoparticles
PublikacjaIn this work, the bulk and surface composition of Fe3O4 supermagnetic nanoparticles were modified for efficient epoxy curing. The bare, ethylenediaminetetraacetic acid (EDTA) capped, and cobalt (Co)-doped EDTA capped Fe3O4 nanoparticles were synthesized electrochemically. The crystalline structure and phase information, surface capping, morphology and magnetization behavior of nanoparticles were studied by X-Ray diffraction (XRD),...
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Thermal and technological aspects of double face grinding of Al2O3 ceramic materials
PublikacjaDouble face grinding with planetary kinematics is a process to manufacture workpieces with plan parallel functional surfaces, such as bearing rings or sealing shims. In order to increase the economic efficiency of this process, it has to be advanced permanently. The temperature in the contact zone of most grinding processes has a huge influence on the process efficiency and the workpiece qualities. In contrast to most grinding...
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Polychlorinated Biphenyls (PCBs) and Polycyclic Aromatic Hydrocarbons (PAHs): Sediments and Water Analysis
PublikacjaUnfavorable side effects of different forms of anthropogenic activities can be found anywhere in the world. One of the basic characteristics of pollutants entering marine and ocean waters is their spread and movement in the global ocean. A portion of the substances entering the marine environment is rapidly degraded by chemical processes occurring in the air, sediments, and water, thereby losing their toxic properties. The biggest...
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Toxoplasma gondii Recombinant antigen AMA1: Diagnostic Utility of Protein Fragments for the Detection of IgG and IgM Antibodies
PublikacjaToxoplasma gondii is an important zoonotic protozoan that infects a wide variety of vertebrates as intermediate hosts. For this reason, the diagnosis of this disease is very important and requires continuous improvement. One possibility is to use recombinant antigens in serological tests. Apical membrane antigen 1 (AMA1), a protein located in specific secretory organelles (micronemes) of T. gondii, is very interesting in regard...
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Filter-Hilbert Method for Automatic Correction of Non-Anechoic Antenna Measurements with Embedded Self-Calibration Mechanism
PublikacjaOne of the most important steps in the process of antenna development involves measurements of its prototype. Far-field performance of radiators is normally characterized in strictly controlled environments such as anechoic chambers which can ensure certification-grade accuracy. Unfortunately, they are also characterized by high construction costs which might not be justified for low-budget research and/or teaching-related activities....
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Ongoing progress on novel nanocomposite membranes for the separation of heavy metals from contaminated water
PublikacjaMembranes, as the primary separation element of membrane-based processes, have greatly attracted the attention of researchers in several water treatment applications, including wastewater treatment, water purification, water disinfection, toxic and non-toxic chemical molecules, heavy metals, among others. Today, the removal of heavy metals from water has become challenging, in which chemical engineers are approaching new materials...
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Synthesis of Trehalose by the Erythritol-Producing Yeast Yarrowia lipolytica Co-Displaying Maltooligosyltrehalose Synthase and Maltooligosyltrehalose Trehalohydrolase
PublikacjaIndustrial trehalose production faces economic challenges with costly enzyme preparations, prompting the exploration of eco-friendly alternatives. Here, we established a coupled functional sugar production line leveraging erythritolproducing cells as an innovative enzyme preparation for trehalose synthesis. The erythritol-producing Yarrowia lipolytica was modified to express a fusion protein consisting of maltooligosyltrehalose synthase...
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Ciliated protozoa in the impact zone of the Uzhgorod treatment plant
PublikacjaThis paper presents the results of studies on the Uzh River (Ukraine, Zakarpattia Oblast) near the effluent point of a sewage treatment plant in Uzhgorod. The samples were taken at various sites of the treatment plant along the stages of purification process, as well as in the river, at a number of different points above and below the wastewater discharge. At each of these objects, the temperature...
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Role of miR-15b/16–2 cluster network in endometrial cancer: An in silico pathway and prognostic analysis
PublikacjaEndometrial cancer (EC) is the second most common cancer in women. A large number of human cancers exhibit dysregulation of microRNA expression including EC. MiR-15b/16–2 is one of the best-known miRNA clusters that is expressed in many types of cancer tissues. Herein, we analyzed the expression of individual miR-15b/16–2 cluster members, its paralogues, and their target network analysis, as well as their prognostic significance...
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Dynamic host configuration protocol for IPv6 improvements for mobile nodes
PublikacjaIn wireless networks mobile clients change their physical location, which results in changing point of attachment to the network. Such handovers introduce unwanted periods, when node does not have communication capabilities. Depending on many conditions, such events may require reconfiguration of layer 2 (e.g. IEEE 802.16) or both 2 and 3 layers (IPv6). This paper investigates delays introduced in the latter type of handover. IPv6...
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Extending the Frequency Limit of Microstrip-Coupled CSRR Using Asymmetry
PublikacjaAbstract— This article explains the frequency limitation in designing microstrip circuits based on a complementary split-ring resonator (CSRR) and reports a novel technique for increasing its operating frequency, which makes the CSRR suitable for high-frequency applications. This study helps in synthesizing the dimensions of symmetric CSRR (SCSRR) and asymmetric CSRR (ACSRR) circuits, which shows the applicability of the proposed...
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Optimization-Based Robustness Enhancement of Compact Microwave Component Designs with Response Feature Regression Surrogates
PublikacjaThe ability to evaluate the effects of fabrication tolerances and other types of uncertainties is a critical part of microwave design process. Improving the immunity of the device to parameter deviations is equally important, especially when the performance specifications are stringent and can barely be met even assuming a perfect manufacturing process. In the case of modern miniaturized microwave components of complex topologies,...
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Expedited Yield-Driven Design of High-Frequency Structures by Kriging Surrogates in Confined Domains
PublikacjaUncertainty quantification is an important aspect of engineering design, also pertaining to the development and performance evaluation of high-frequency structures systems. Manufacturing tolerances as well as other types of uncertainties, related to material parameters (e.g., substrate permittivity) or operating conditions (e.g., bending) may affect the characteristics of antennas or microwave devices. For example, in the case...
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Expedited Machine-Learning-Based Global Design Optimization of Antenna Systems Using Response Features and Multi-Fidelity EM Analysis
PublikacjaThe design of antenna systems poses a significant challenge due to stringent per-formance requirements dictated by contemporary applications and the high com-putational costs associated with models, particularly full-wave electromagnetic (EM) analysis. Presently, EM simulation plays a crucial role in all design phases, encompassing topology development, parametric studies, and the final adjustment of antenna dimensions. The latter...
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The issue of uncertainty of visual measurement techniques for long distance measurements based on the example of applying electric traction elements in diagnostics and monitoring
PublikacjaRail transport is the most economical and energy-effective in the field of land transport, in particular electrified. In order to ensure efficient and reliable operation of electrified rail transport, the issues of monitoring and diagnostics of the traction infrastructure and vehicles are extremely important. The most critical point in the transmission of electric energy to the vehicle is the sliding contact of the current collector...
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Wydajność przetwarzania żądań usług uwarunkowanych czasowo realizowanych w sieci IMS/NGN
PublikacjaW rozprawie dokonano przeglądu stanu implementacji koncepcji IMS/NGN, a także modeli systemów obsługi z oczekiwaniem pod kątem zastosowania dla serwerów i łączy w modelu analitycznym wielodomenowej sieci IMS/NGN. Przedstawiono założenia dla tego modelu oraz metodologię obliczeń i analizy wyników: średnich czasów E(CSD) zestawiania i E(CDD) rozłączenia połączenia dla scenariuszy połączeń zakończonych sukcesem. Opisano założenia,...
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Wzrost gospodarczy a zapotrzebowanie na prace - teoria i rzeczywistość gospodarcza Polski w latach 1995-2014
PublikacjaW części teoretycznej artykułu w pierwszej kolejności przedstawiono makroekonomiczne podstawy zapotrzebowania na pracę w warunkach postępu technicznego oraz zmian kapitału rzeczowego. W następnej kolejności sformułowano założenia dla modelu opisującego zależności pomiędzy stopami wzrostu produktu krajowego i zatrudnienia. W części empirycznej artykułu rozważano wybrane wersje oszacowanego modelu opisujące gospodarkę Polski. Do...
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Rapid method for the determination of organochlorine pesticides and PCBs in fish muscle samples by microwave-assisted extraction and analysis of extracts by GC-ECD
PublikacjaA procedure for the multiresidue determination of organochlorine pesticides and polychlorinated biphenyls in fish muscle samples has been developed. The method is based on the microwave-assisted extraction (MAE) of food samples from an acetonitrile-water (95 + 5, v/v) mixture followed by SPE cleanup of the extracts and analysis by GC with an electron capture detector. MAE operational parameters, such as the extraction solvent,...
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Deep eutectic solvents – A new platform in membrane fabrication and membrane-assisted technologies
PublikacjaDeep eutectic solvents (DESs) are a new class of solvents that can offset some of the primary drawbacks of typical solvents and ionic liquids (ILs). Since DESs fall into the guidelines of “Twelve Principles of Green Chemistry”, their implementation in several types of applications has exponentially increased over the last years. The usage of DESs has been directed to the designing, manufacture and purification of new materials...
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Phosphorus and nitrogen forms in liquid fraction of digestates from agricultural biogas plants
PublikacjaThe novelty of the presented research is the determination of the nitrogen and phosphorus fraction in the liquid fraction of digestate from agricultural biogas plants. This information is important because it can help in proposing possible further liquid fraction management or developing of new technologies for their purification. The research covered digestates from agricultural biogas plants, obtained from the fermentation of...
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Reduction of CO2 Emissions from Offshore Combined Cycle Diesel Engine-Steam Turbine Power Plant Powered by Alternative Fuels
PublikacjaDiverse forms of environmental pollution arise with the introduction of materials or energy that exert adverse effects on human health, climate patterns, ecosystems, and beyond. Rigorous emission regulations for gases resulting from fuel combustion are being enforced by the European Union and the International Maritime Organization (IMO), directed at maritime sectors to mitigate emissions of SOx, NOx, and CO2. The IMO envisions...
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Stannates, titanates and tantalates modified with carbon and graphene quantum dots for enhancement of visible-light photocatalytic activity
PublikacjaMost efforts in heterogeneous photocatalysis are focused on development of new and stable photoactive materials efficient in degradation of various pollutants under visible-light irradiation. In this regard, the wide-bandgap perovskite semiconductors, i.e., SrTiO3 (titanate), SrSnO3 (stannate) and AgTaO3 (tantalate), were prepared by a solvothermal method, and then modified with carbon quantum dots (CQDs) or graphene quantum dots...
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A new factor LapD is required for the regulation of LpxC amounts and lipopolysaccharide trafficking
PublikacjaLipopolysaccharide (LPS) constitutes the major component of the outer membrane and is essential for bacteria, such as Escherichia coli. Recent work has revealed the essential roles of LapB and LapC proteins in regulating LPS amounts; although, if any additional partners are involved is unknown. Examination of proteins co-purifying with LapB identified LapD as a new partner. The purification of LapD reveals that it forms a complex...
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Laser granulometer as an useful tool for selection of appropriate membranes used in the MIEX®DOC-UF/MF hybrid process
PublikacjaIn the study, particle size distribution of the MIEX® resin was presented. Such analyses enable to determinate whether presence of fine resin fraction may be the reason for unfavorable membrane blocking during water purification by the hybrid MIEX®DOC-microfiltration/ultrafiltration systems. Granulometric analysis of resin grains using the laser diffraction particle size analyzer (laser granulometer) was carried out as well as...
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Single-phase product obtained via crude glycerine depolymerisation of polyurethane elastomer: structure characterisation and rheological behaviour
PublikacjaPolyurethane recycling is a topic of growing interest due to the increasing amount of polyurethanewaste. The main purpose of polyurethane feedstock recycling is to recover the starting polyol, a valuable material. In thiswork, amethod of polyurethane thermo-chemical recycling, glycerolysisbymeansof crude glycerine, is proposed. Themaineffort is focusedonthe employment of crude glycerine without purification from biodiesel production...
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Assessing and Mitigating Ice-Jam Flood Hazards and Risks: A European Perspective
PublikacjaThe assessment and mapping of riverine flood hazards and risks is recognized by many countries as an important tool for characterizing floods and developing flood management plans. Often, however, these management plans give attention primarily to open-water floods, with ice-jam floods being mostly an afterthought once these plans have been drafted. In some Nordic regions, ice-jam floods can be more severe than open-water floods,...
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Dissecting gamma frequency activities during human memory processing
PublikacjaGamma frequency activity (30-150 Hz) is induced in cognitive tasks and is thought to reflect underlying neural processes. Gamma frequency activity can be recorded directly from the human brain using intracranial electrodes implanted in patients undergoing treatment for drug-resistant epilepsy. Previous studies have independently explored narrowband oscillations in the local field potential and broadband power increases. It is not...
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Optimized AVHRR land surface temperature downscaling method for local scale observations: case study for the coastal area of the Gulf of Gdańsk
PublikacjaSatellite imaging systems have known limitations regarding their spatial and temporal resolution. The approaches based on subpixel mapping of the Earth’s environment, which rely on combining the data retrieved from sensors of higher temporal and lower spatial resolution with the data characterized by lower temporal but higher spatial resolution, are of considerable interest. The paper presents the downscaling process of the land...