Wyszukiwarka
Filtry
wszystkich: 2712
wybranych: 2422
-
Katalog
- Publikacje 2422 wyników po odfiltrowaniu
- Czasopisma 44 wyników po odfiltrowaniu
- Konferencje 1 wyników po odfiltrowaniu
- Osoby 51 wyników po odfiltrowaniu
- Wynalazki 5 wyników po odfiltrowaniu
- Projekty 2 wyników po odfiltrowaniu
- Zespoły Badawcze 1 wyników po odfiltrowaniu
- Kursy Online 43 wyników po odfiltrowaniu
- Wydarzenia 25 wyników po odfiltrowaniu
- Dane Badawcze 118 wyników po odfiltrowaniu
Filtry wybranego katalogu
wyświetlamy 1000 najlepszych wyników Pomoc
Wyniki wyszukiwania dla: TAQ POLYMERASE
-
Increased concentration of Taq DNA polymerase as a solution for GC-rich templates from clinical and environmental samples
PublikacjaDNA polymerase is an enzyme which plays crucial role in replication and DNA repair. It found application in PCR (polymerase chain reaction) where catalyses process of in vitro DNA synthesis. To meet the demands posed by mod- ern diagnostic, molecular biology or genetic engineering it is necessary to improve DNA polymerases to obtain new or better features useful in these fields. So far implemented modifications in majority are...
-
Taq DNA polymerase fused with DNA binding protein with increased resistance to inhibitors from clinical samples
PublikacjaNowadays PCR method is commonly used in molecular diagnostic. However, in many cases PCR is limited, by the presence of inhibitory substances in biological, soil or food samples Efficiency and fidelity of amplification is strongly connected with DNA polymerase and reaction conditions. To meet the requirements of modern diagnostic methods it is essential to seeking for new DNA polymerases with better properties useful in these field....
-
Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans—Expression and characterization
PublikacjaDNA polymerases are present in all organisms and are important enzymes that synthesise DNA molecules. They are used in various fields of science, predominantly as essential components for in vitro DNA syntheses, known as PCR. Modern diagnostics, molecular biology and genetic engineering need DNA polymerases which demonstrate improved performance. This study was aimed at obtaining a new NeqSSB-TaqS fusion DNA polymerase from the...
-
Fuzyjne polimerazy DNA – otrzymywanie, charakterystyka i zastosowanie
PublikacjaObecnie reakcje PCR (ang. Polymerase Chain Reaction) wykazują bardzo szerokie zastosowanie w diagnostyce medycznej, biologii molekularnej czy inżynierii genetycznej. Efektywność tych reakcji rozumiana jako wydajność i wierność przeprowadzonej amplifikacji jest nieodłącznie związana ze stosowaną polimerazą DNA i warunkami prowadzenia reakcji PCR. Aby sprostać wymaganiom stawianym przez nowoczesne metody diagnostyczne oraz współczesną...
-
Modified DNA polymerases for PCR troubleshooting
PublikacjaPCR has become an essential tool in biological science. However, researchers often encounter problems with difficult targets, inhibitors accompanying the samples, or PCR trouble related to DNA polymerase. Therefore, PCR optimization is necessary to obtain better results. One solution is using modified DNA polymerases with desirable properties for the experiments. In this article, PCR troubleshooting, depending on the DNA polymerase...
-
Detection of Blastocystis hominis in unpreserved stool specimens byusing polymerase chain reaction
PublikacjaBlastocystis hominis jest pasożytem występującym na całym świecie. Patogenność tego pasożyta nie jest jeszcze w pełni udowodniona, aczkolwiek opisanych jest wiele przypadków, sugerujących, że B. hominis może wywoływać objawy żołądkowo-jelitowe. Wykrywanie tego pasożyta opiera się na bezpośredniej obserwacji mikroskopowej rozmazów i zagęszczonych próbek kału lub rozmazów utrwalonych, jednakże w pewnych przypadkach nie jest to możliwe.Celem...
-
Mechanism of reaction of RNA-dependent RNA polymerase from SARS-CoV-2
PublikacjaWe combine molecular dynamics, statistical mechanics, and hybrid quantum mechanics/molecular mechanics simulations to describe mechanistically the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA-dependent RNA polymerase (RdRp). Our study analyzes the binding mode of both natural triphosphate substrates as well as remdesivir triphosphate (the active form of drug), which is bound preferentially over ATP by RdRp while...
-
What affects the biocompatibility of polymers?
Publikacja -
Shape-Memory Polyurethane Polymers
PublikacjaPolyurethanes (PUs) are one of the most widely used polymers in research and industry. They can be synthesized from chemical sources and natural sources. PUs are a very useful class of polymers and exhibit many desirable properties that can be exploited in various applications. PUs are formed by the reaction between polyols and isocyanates. A wide variety of polyols and isocyanates are available for synthesis, and hence we can...
-
Rheology of liquid crystalline polymers
PublikacjaLiquid crystallinity was invented in the 19th century. It was sometimes called the fourth state of matter because of the ability of macromolecules to generate liquid crystal phases. The main classification divided liquid crystalline polymers (LCPs) into lyotropic and thermotropic types. Interpretation of the rheological behavior of LCP is more complicated than for other polymers. Ordinary polymers are characterized by a normal...
-
Conducting polymers for biodegradable metallic implants
PublikacjaNowadays permanent metallic cardiovascular stents are long-term implants. The long-time presence of such an implant in human body can cause overgrowth of tissue within the treated portion of the vessel, blockage of the circulatory system and many other clinical complications, such as thrombosis, prolonged physical irritations or chronic inflammation. Therefore, in recent years, there is an interest to create biodegradable metallic...
-
Conducting polymers electrodeposited on active metals
PublikacjaPraca poświęcona studiom literaturowym doniesień dotyczących polimerów przewodzących, osadzanych na metalach aktywnych. Zastosowanie polimerów przewodzących jako warstw antykorozyjnych oraz elektrokrystalizacja metali na polimerach przewodzących zostały zebrane i przedstawione w tej pracy.
-
The Escherichia coli RNA polymerase alpha subunit and transcriptional activation by bacteriophage lambda CII protein.
Publikacja -
A simple modification to improve the accuracy of methylation-sensitive restriction enzyme quantitative polymerase chain reaction
PublikacjaDNA digestion with endonucleases sensitive to CpG methylation such as HpaII followed by polymerase chain reaction (PCR) quantitation is commonly used in molecular studies as a simple and inexpensive solution for assessment of region-specific DNA methylation. We observed that the results of such analyses were highly overestimated if mock-digested samples were applied as the reference.We determined DNA methylation levels in several...
-
''Tak naprawdę''
PublikacjaPrzeanalizowano kwestię zakłamania w dzisiejszym życiu społecznym i politycznym
-
Synthesis and electrochemical characteristics of novel dithiocarbamate polymers
PublikacjaNovel dithiocarbamate compounds have been obtained by reacting a linear poly(ethylenimine)polymer, MW 50,000, with CS2. The resulting anionic polymers contain, depending on the reaction conditions, up to two sulfur atoms per one nitrogen atom (approx. 40% w/w). The polymers are soluble in dimethylsulfoxide and insoluble in acetonitrile solutions. In both solvents they are electrochemically active, in the latter case as a cast film...
-
Swelling effects in cross-linked polymers by thermogravimetry
Publikacja -
Recent progress in controlled carbonization of (waste) polymers
Publikacja -
Dispersion of thermo-optic coefficient of optical polymers
Publikacja -
Synthesis and polymerisation techniques of molecularly imprinted polymers
PublikacjaMolecularly Imprinted Polymers (MIPs) are materials that has been processed using the molecular imprinting technique which permit to obtain well-defined three-dimensional cavities, with affinity to a template molecule, in the polymer matrix. Technology involves three strategies, i.e., covalent, non-covalent and semi-covalent approach, but the most popular is non-covalent approach. The most important components for the synthesis...
-
Electroconducting polymers as a corrosion protection materials review
PublikacjaAutorzy przywołują 89 pozycji literaturowych dotyczących użycia polimerów elektroaktywnych w ochronie metali przed korozją. Wykazano, że najczęściej wykorzystywany w tym celu jest polipirol. Przytoczone prace dotyczą ochrony stali, aluminium, cynku oraz stopów magnezu.
-
Hydrogels Based on Natural Polymers for Cardiac Applications
PublikacjaIn this work agar- and borax-based hydrogels with and without the addition of poly(vinyl alcohol) (PVA) at different concentrations were synthesized. Hydrogels were modified by the same amount of acetylsalicylic acid (ASA) which exhibits antithrombotic properties. The effect of modification by ASA on the properties of hydrogels was analyzed.
-
Fusion of DNA-binding domain of Pyrococcus furiosus ligase with TaqStoffel DNA polymerase as a useful tool in PCR with difficult targets
PublikacjaThe DNA coding sequence of TaqStoffel polymer- ase was fused with the DNA-binding domain of Pyrococcus furiosus ligase. The resulting novel recombinant gene was cloned and expressed in E. coli. The recombinant enzyme was purified and its enzymatic features were studied. The fusion protein (PfuDBDlig-TaqS) was found to have enhanced processivity as a result of the conversion of the Taq DNA polymerase from a relatively low processive...
-
Novel Biomass-Based Polymers: Synthesis, Characterization, and Application
PublikacjaA wide range of polymers were prepared from biomass-derivatives, using different polymerization mechanisms. Well-defined, fully hydroxy-functional polyesters based on aliphatic diols were synthesized, using either conventional metal-based catalysts or the organic superbase 1,5,7-triazabicyclododecene (TBD). Unsaturated polyesters were also made, offering additional functionality to these biobased resins. Metal-catalyzed or enzymatic...
-
Polymers in High-Efficiency Solar Cells: The Latest Reports
Publikacja -
Gas sensors based on conducting polymers-recent developments
PublikacjaThis work discusses sensing performance dependence of PEDOT polymer and its composites on the counter ions used in the polymerization process. The sensors based on PEDOT-RGO composite show reversible response to NO2, while on PEDOT/LiClO4 irreversible. As a result, PEDOT-RGO could be used as a typical gas sensor, while sensor based PEDOT/LiClO4 could be used as an integrating gas sensor, also known as an accumulating gas sensor....
-
Marine polymers in tissue bioprinting: Current achievements and challenges
PublikacjaBioprinting has a critical role in tissue engineering, allowing the creation of sophisticated cellular scaffolds with high resolution, shape fidelity, and cell viability. Achieving these parameters remains a challenge, necessitating bioinks that are biocompatible, printable, and biodegradable. This review highlights the potential of marine-derived polymers and crosslinking techniques including mammalian collagen and gelatin along...
-
The suitability of DNA extracted from formalin-fixed, paraffin-embedded tissues for double differential polymerase chain reaction analysis
Publikacja -
Tax Evasion, Tax Morale, and Trade Regulations: Company-Level Evidence from Poland
PublikacjaObjective: To investigate the determinants of the phenomenon of evading taxes among polish entrepreneurs. In particular, I examine such factors as tax administration satisfaction, tax morale, tax burden, and the influence of trade regulations. Research Design & Methods: A survey study conducted in 2017 in Poland among 454 enterprises. I used the zero-inflated negative binomial modelling technique to examine the impact of factors...
-
Theoretical study of polymerization mechanism of p-xylylene based polymers
PublikacjaObliczenia kwantowe mechanizmu polimeryzacji parylenów, opartych o cząsteczki p-ksylilenów. Praca zawiera wyniki szczegółowych obliczeń wszystkich etapów polimeryzacji, wnioski dotyczące znalezionych stanów kwantowych i energetyki poszczególnych etapów procesu.
-
Photovoltaic bulk heterojunctions with interpenetrating network based on semiconducting polymers
PublikacjaPhotovoltaic cells are supposed to be the most common generators of useful electricity in the nearest future as they utilize inexhaustible carriers of renewable energy called photons. Despite the fact that 95% of worldwide applied photovoltaic devices are based on inorganic semiconductors the area of organic photovoltaics grows successively due to possibility of considerable reduction of manufacturing costs. Since the Heeger's...
-
Progress in development of molecularly imprinted polymers as sorbents for sample preparation
PublikacjaSample preparation has been one of the most often investigated steps in analytical procedures.These investigations are focused on problems such as isolation of analytes from the sample matrix, matrix implification, analyte enrichment to the level above the detection limit of the instrumentation used, and the removal of interfering species to improve the final determination. Techniques based on solid (adsorption) and pseudo liquid...
-
Analysis of structure and properties of DVB–GMA based porous polymers
Publikacja -
Sorption Properties of Polydivinylbenzene Polymers towards Phenolic Compounds and Pharmaceuticals
Publikacja -
Polymers (Polyvinylpyrrolidones) As Active Additives Modifying the Lubricating Properties of Water
Publikacja -
Theoretical Study of Polymerization Mechanism of p-Xylylene Based Polymers
Publikacja -
PU Polymers, Their Composites, and Nanocomposites: State of the Art and New Challenges
PublikacjaThe chapter is a review of the polyurethane(PU)-based composites and nanocomposites. The chapter details different components in the manufacturing of PU polymers, composites, and nanocomposites. The different types of glycols, isocyanates, and catalysts that are used for the synthesis of different polyurethanes are discussed. The applications and specialties of polyurethane composites and nanocomposites are also elaborated on.
-
Hybrid nanolayers of star polymers and silver nanoparticles with antibacterial activity
Publikacja -
The Role of Specific Side Groups and pH in Magnetization Transfer in Polymers
Publikacja -
Raman investigation of sol-gel-derived hybrid polymers for optoelectronics
PublikacjaPrzedstawiono wykorzystanie spektroskopii ramanowskiej do kompleksowej diagnostyki polimerów hybrydowych stosowanych w optoelektronice. Zbadano efektywność kolejnych etapów procesu technologicznego: od monomerów do etapu produktu finalnego - cienkich warstw.
-
Application of molecularly imprinted polymers in analytical chiral separations and analysis
PublikacjaOver the last two decades the process of development and application of a new types of molecular imprinted polymer (MIP) sorbents in the field of analytical chemistry have been widely described in the literature. One of the new trends in analytical chemistry practice is the use of new types of MIP sorbents as specific sorption materials constituting the stationary phase in advanced separation techniques. The following review paper...
-
Sustainable polymers targeted at the surgical and otolaryngological applications: Circularity and future
PublikacjaThe ongoing climate changes, high air and noise pollution have significant impact on humans’ health. This influence is especially visible in otolaryngology, which focuses on respiratory and hearing systems disfunctions. However, even though surgeries are done in response to diseases related to climate changes, they also have a negative impact on the environment, mostly connected with the inherence of single-use fossil fuel derived...
-
Molecularly imprinted polymers applied in capillary electrochromatography and electrophoresis techniques
PublikacjaMolecularly imprinted polymers (MIPs) are synthetic materials with predetermined selectivity for a particular analyte or group of structure-linked chemicals which make them an ideal separation component. The process of developing and applying new types of MIPs in the field of environmental analytical chemistry has been broadly discussedi n many scientific studies in recent years. The use of new types of MIP sorbents as unique sorption...
-
Bringing a New Flexible Mercaptoacetic Acid Linker to the Design of Coordination Polymers
Publikacja -
Star Polymers vs. Dendrimers: Studies of the Synthesis Based on Computer Simulations
Publikacja -
The use of fibre-reinforced polymers (FRP) in bridges as a favourable solution for the environment
PublikacjaThe purpose of this article is to show the modern engineering, in which sustainability and taking care of ecology play a significant role. The authors are focused on FRP composite materials and their applications in civil engineering. Case studies showing renovation and design of new bridges with the use of FRP are presented and discussed to clarify benefits, which this solution provides. Main advantages of FRP materials in comparison...
-
The Application of Phenolic Acids in The Obtainment of Packaging Materials Based on Polymers—A Review
Publikacja -
Modelling of optical components made of liquid crystals and liquid crystalline polymers
PublikacjaW artykule przedstawiono koncepcję wykorzystania ciekłych kryształów i polimerów ciekłokrystalicznych do budowy dwójłomnych elementów optycznych - płytek opóźniających, pryzmatów Wollastona i Nomarskiego. Przedstawiono wyniki modelowania pryzmatów Nomarskiego wykonanych z polimerów ciekłokrystalicznych i dokonano porównania ich właściwości z właściwościami pryzmatów wykonanych z typowych materiałów dwójłomnych.
-
A new concept of measuring the friction forces for cartilage and polymers with viscoelastic properties
PublikacjaReferat przedstawia nową koncepcję dotyczącą pomiaru niskich sił tarcia oraz właściwości materiałów lepkosprężystych. Zaprezentowano: ogólny zarys metody pomiaru, wskazania dotyczące projektowania i budowy urządzenia pomiarowego, model matematyczny oraz sposób identyfikacji parametrów modelu. Metoda ta jest szczególnie przydatna w przypadku analizy właściwości materiałowej polimerów oraz tkanki chrząstki stawowej człowieka, zarówno...
-
The Importance of Interferon-Tau in the Diagnosis of Pregnancy
Publikacja