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DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation
PublikacjaA new diagnostic kit for K. oxytoca specific differentiation based on ddLMS PCR (ang. double digest ligation Mediated PCR) technique is shown. As a species-specific DNA fragment pehX gene, encoding the enzyme polygalactouronase, was chosen. The genome sequence of K. oxytoca is digested with two endonucleases: AclI and BclI which cut DNA before and after pehX gene. The polymorphic DNA fragments are ligated with AclI-end-specific...
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Acoustic emission studies of the 7075 aluminium alloy pitting corrosion process
Dane BadawczeThe dataset contains the cyclic polarization studies and corresponding acoustic emission measurements, revealing the pitting corrosion process of the passive layer at the surface of the 7075 aluminum alloy in the borate buffer with different chloride ions concentrations, in the range between 0.05 and 25 mM.
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Loop-mediated isothermal amplification (LAMP) as a diagnostic tool in detection of infectious diseases
PublikacjaLoop-mediated isothermal amplification (LAMP) is a gene amplification method which amplifies DNA with high specificity and efficiency under isothermal conditions. Because of its rapidity and simplicity, it is a valuable diagnostic tool in the early detection and identification of infectious diseases. LAMP method is based on the use of a set of four to six specially designed primers spanning six to eight distinct sequences on the...
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Excitation-independent constant conductance isfet driver
PublikacjaA new constant conductance driver for ISFETs sensors has been developed. The proposed circuit maintains the sensor operating point at constant drain-source conductance. The combination of a simple, self-balancing resistance bridge and the subtraction half (or similar fraction) of source-drain voltage from the gate-source voltage provides the independence of output signal from current and voltage drivers instability. The use of...
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PCR-RFLP assays for species-specific identification of fungi belonging to Scopulariopsis and related genera
PublikacjaFungi of the Scopulariopsis genus, commonly found in the environment, are opportunistic pathogens that can cause various types of human infections. So far, no efficient molecular method has been developed for species differentiation among Scopulariopsis and related genera. In order to advance this field, we have evaluated performance of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assays, based...
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Multiple output differential OTA with linearizing bulk-driven active-error feedback loop for continuous-time filter applications
PublikacjaA CMOS circuit realization of a highly linear multiple-output differential operational transconductance amplifier (OTA) has been proposed. The presented approach exploits a differential pair as an input stage with both the gate and the bulk terminals as signal ports. For the proposed OTA, improved linearity is obtained by means of the active-error feedback loop operating at the bulk terminals of the input stage. SPICE simulations...
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A novel microstrip dual-layer rat-race coupler with compact size and enhanced bandwidth
PublikacjaMicrowave hybrid couplers are crucial components of mixers, phase shifters, amplifiers and other high-frequency systems. Conventional couplers are characterized by large size which limits their usefulness in modern applications. In this work, a novel compact rat-race coupler with enhanced bandwidth has been proposed. The structure consists of six compact microstrip resonant cells. It is implemented on two separate layers which permits...
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Genotyping of clinical isolates of fluconazole-resistant Candida albicans
PublikacjaThe aim of the study was to compare the discriminatory power of RAPD-PCR method with using RSD10 primer and microsatellite analysis with using (GACA)4 primer for genotyping clinical isolates of fluconazole-resistant C. albicans. Isolates were received from patients of Children's Memorial Health Institute in Warsaw. However in the case of both tested methods low number of amplified fragments was generated, nine and six different...
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Technique to improve CMRR at high frequencies in CMOS OTA-C filters
PublikacjaIn this paper a technique to improve the common-mode rejection ratio (CMRR) at high frequencies in the OTA-C filters is proposed. The technique is applicable to most OTA-C filters using CMOS operational transconductance amplifiers (OTA) based on differential pairs. The presented analysis shows that a significant broadening of CMRR bandwidth can be achieved by using a differential pair with the bodies of transistors connected to...
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Digital microcontroller for sonar waveform generator
PublikacjaGenerating sounding signals is essential for the operation of active sonar. The system should be highly reliable. This can be achieved through architecture, communication between the devices, and a well-designed and self–testing software. The system presented in the article is responsible for the generation of hydroacoustic sounding signals, and ensures proper interaction between power amplifiers and power supplies. Thanks to its...
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The role of EMG module in hybrid interface of prosthetic arm
PublikacjaNearly 10% of all upper limb amputations concern the whole arm. It affects the mobility and reduces the productivity of such a person. These two factors can be restored by using prosthetics. However, the complexity of human arm makes restoring its basic functions quite difficult. When the osseointegration and/or targeted muscle reinnervation (TMR) are not possible, different modalities can be used to control the prosthesis. In...
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ReFlexeNN - the Wearable EMG Interface with Neural Network Based Gesture Classification
PublikacjaThe electromyographic activity of muscles was measured using a wireless biofeedback device. The aim of the study was to examine the possibility of creating an automatic muscle tension classifier. Several measurement series were conducted and the participant performed simple physical exercises - forcing the muscle to increase its activity accordingly to the selected scale. A small wireless device was attached to the electrodes placed...
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Properties of recombinant trehalose synthase from Deinococcus radiodurans expressed in Escherichia coli
PublikacjaA trehalose synthase gene from Deinococcus radiodurans(DSMZ 20539)containing 1659 bp reading frame encoding 552 amino acids was amplified using PCR. The gene was finally ligated into pET30Ek/LIC vector and expressed after isopropyl β-d-thiogalactopyranoside induction in Escherichia coli (DE3) Rosetta pLysS. The recombinanttrehalose synthase (DraTreS) containing a His6-tag at the C-terminus was purified by metal affinity chromatography...
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Fusion of DNA-binding domain of Pyrococcus furiosus ligase with TaqStoffel DNA polymerase as a useful tool in PCR with difficult targets
PublikacjaThe DNA coding sequence of TaqStoffel polymer- ase was fused with the DNA-binding domain of Pyrococcus furiosus ligase. The resulting novel recombinant gene was cloned and expressed in E. coli. The recombinant enzyme was purified and its enzymatic features were studied. The fusion protein (PfuDBDlig-TaqS) was found to have enhanced processivity as a result of the conversion of the Taq DNA polymerase from a relatively low processive...
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Realistic noise-tolerant randomness amplification using finite number of devices
PublikacjaRandomness is a fundamental concept, with implications from security of modern data systems, to fundamental laws of nature and even the philosophy of science. Randomness is called certified if it describes events that cannot be pre-determined by an external adversary. It is known that weak certified randomness can be amplified to nearly ideal randomness using quantum-mechanical systems. However, so far, it was unclear whether randomness amplification...
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Low cost set-up for supercapacitors parameters evaluation
PublikacjaSupercapacitors are capable to store relatively high amount of energy comparing to its mass. Growing number of these devices applications requires development of new testing methods. Standard methods of evaluation of supercapacitor parameters, as cycling voltammetry, CV, galvanostatic cycling with potential limitation, GCPL, impedance measurements, require equipment of high cost...
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Mucinous carcinoma - Female, 69 - Tissue image [5040730020275231]
Dane BadawczeThis is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Mucinous carcinoma - Female, 69 - Tissue image [5040730020277561]
Dane BadawczeThis is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Mucinous carcinoma - Female, 69 - Tissue image [5040730020274231]
Dane BadawczeThis is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Mucinous carcinoma - Female, 69 - Tissue image [5040730020274161]
Dane BadawczeThis is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Towards classification of patients based on surface EMG data of temporomandibular joint muscles using self-organising maps
PublikacjaThe study considers the need for an effective method of classification of patients with a temporomandibular joint disorder (TMD). The self-organising map method (SOM) was applied to group patients and used together with the cross-correlation approach to interpret the processed (rectified and smoothed by using root mean square (RMS) algorithm) surface electromyography signal (sEMG) obtained from testing the muscles (two temporal...
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The shallow sea experiment with usage of linear hydrophone array
PublikacjaPurpose of this article is to present designed and made linear hydrophone array and the results obtained during in situ trails on Gulf of Gdańsk. The measuring system allowed to localize hydrophones in the selected points and perform measurements in both the horizontal antenna positioning and vertical. Made in this way recordings allow creating accurate 3D imaging of sound intensity/propagation. During research three floating objects...
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A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms
PublikacjaABSTRACT Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic...
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Fully Tunable Analog Biquadratic Filter for Low-Power Auditory Signal Processing in CMOS Technologies
PublikacjaA novel Gm-C structure of a second-order continuous-time filter is proposed that allows for the independent control of the filter’s natural frequency (ω0) and quality factor (Q). The structure consists of two capacitors and four transconductors. Two transconductors together with the capacitors form a lossless second-order circuit with tunable ω0. The other two transconductors form a variable gain amplifier (VGA) which realizes...
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Investigating lubricants behaviour in microgravity for vibration damping purposes
PublikacjaVibration is one of the harshest environments an object is exposed to during and after launch into space on a launch vehicle. Such vibrations should be damped to avoid destruction of the spacecraft or its elements. Currently, active and material damping is used. A possibility of using lubrication as the damping factor is suggested by some research. MoS2 is a typical solid lubricant used in space applications. Its properties vary...
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Expression of Deinococcus geothermalis Trehalose Synthase Gene in Escherichia coli and Its Enzymatic Properties
PublikacjaA novel trehalose synthase gene from Deinococcus geothermalis (DSMZ 11300) containing 1,692 bp reading-frame encoding 564 amino acids was amplified using PCR. The gene was ligated into pET30Ek/LIC vector and expressed after isopropyl alfa-D-thiogalactopyranoside induction in Escherichia coli BL21(DE3)pLysS. The recombinant trehalose synthase (DgeoTreS) containing a His6 tag at the C-terminus was purified by metal affinity chromatography...
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Portable exhaled breath analyzer employing fluctuation-enhanced gas sensing method in resistive gas sensors
PublikacjaThis paper presents a portable exhaled breath analyser, developed to detect selected diseases. The set-up employs resistive gas sensors: commercial MEMS sensors and prototype gas sensors made of WO3 gas sensing layers doped with various metal ingredients. The set-up can modulate the gas sensors by applying UV light to induce physical changes of the gas sensing layers. The sensors are placed in a tiny gas chamber of a volume...
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Deep-Learning-Based Precise Characterization of Microwave Transistors Using Fully-Automated Regression Surrogates
PublikacjaAccurate models of scattering and noise parameters of transistors are instrumental in facilitating design procedures of microwave devices such as low-noise amplifiers. Yet, data-driven modeling of transistors is a challenging endeavor due to complex relationships between transistor characteristics and its designable parameters, biasing conditions, and frequency. Artificial neural network (ANN)-based methods, including deep learning...
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Low frequency nois of supecapacitor
Dane BadawczeDataset contains results of low frequency noise observation during discharge of commercially available supercapacitor, type DRL 2.7V 10F, with a nominal capacitance 10 F and a nominal voltage 2.7 V. Supercapacitor was charged to various voltages and discharged through a resistor. In the experiment the 50 Ohm and 200 Ohm resistors were used. The supercapacitor...
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The New LM-PCR/Shifter Method for the Genotyping of Microorganisms Based on the Use of a Class IIS Restriction Enzyme and Ligation Mediated PCR
PublikacjaThis study details and examines a novel Ligation-Mediated - Polymerase Chain Reaction (LM-PCR) method. Named the LM-PCR/Shifter, it relies on the use of a Class IIS restriction enzyme giving restriction fragments with different 4 base, 5' overhangs, this being the Shifter, and the ligation of appropriate oligonucleotide adapters. A sequence of 4-base, 5' overhangs of the adapter and a 4-base sequence of the 3' end of the primer(s)...
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Highly Reliable Passive RFID-Based Inductor–Capacitor Sensory System Strengthened by Solvatochromism for Fast and Wide-Range Lactate Detection
PublikacjaA passive radio frequency-identification-based inductor-capacitor (LC) lactate sensory system with a specific dye-containing interdigitated capacitor (IDC) in which the sensing signal is amplified by the solvatochromic effect is proposed. When a lactate solution contacts the IDC of the LC lactate sensor, the capacitance of the IDC changes, changing the resonance frequency of the sensor. This changes the oscillation frequency of...
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Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms
PublikacjaA significant number of DNA-based techniques has been introduced into the field of microorganisms’ characterization and taxonomy. These genomic fingerprinting methods were developed to detect DNA sequence polymorphisms by using general principles, such as restriction endonuclease analysis, molecular hybridization, and PCR amplification. In recent years, some alternative techniques based on ligation of oligonucleotide adapters before...
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A nosocomial outbreak of Candida parapsilosis in southern Sweden verified by genotyping
PublikacjaIn a haematology ward, Candida parapsilosis was found in blood cultures from 4 patients within a month. As C. parapsilosis is known to have a restricted genetic diversity, a combined methodological approach was adopted to establish a possible epidemiological relationship among the isolates (n = 9). Multilocus sequence typing and random amplified polymorphic DNA analysis suggested a clonal origin of the isolates. The clonal origin...
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A New Rehabilitation Device for Balance Impaired Individuals
PublikacjaIn the paper authors present a device designed to improve the rehabilitation process of people with balance impairment. The discussed device (JStep) utilizes a commercially available static standing frame (stander) modified in order to fit force sensing units under the feet and in the pillows around the hips of a patient. While executing rehabilitation tasks, the patient may compensate his balance deficiency by leaning on the pillows...
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Formaldehyde detection with chemical gas sensors based on WO3 nanowires decorated with metal nanoparticles under dark conditions and UV light irradiation
PublikacjaWe report results of formaldehyde gas (CH2O) detection under dark conditions and UV light irradiation with pristine tungsten trioxide nanowires (WO3 NWs) and metal nanoparticles decorated WO3 NWs gas sensing layers. The resistive layers were deposited by one step aerosol assisted chemical vapor deposition (AACVD) on commercial alumina substrates with 10-pair interdigitated platinum electrodes. The elaborated gas sensors, based...
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Voltage fluctuations on the main switchgear of the industrial power system supplying the rolling mill motors
Dane BadawczeThe dataset presents the voltage waveforms on the bus bars of the main switchgear of the industrial power network for the supply of rolling mills. The data was recorded during an experiment whose purpose was to determine a level of short-term and long-term flicker caused by voltage fluctuations. In the virtual application of flickermeter, a hardware...
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The conducted immunity test of a power supply unit in accordance with EMC standards
Dane BadawczeThe dataset presents a result of measurements that are a part of immunity tests to conducted disturbances, induced by radio-frequency fields. The immunity tests were carried out on the mains cable of the DF1723003TC NDN power supply. Tests of immunity of electronic systems to conducted disturbances in the frequency range from 150 kHz to 230 MHz are...
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The New LM-PCR/Shifter Method for the Genotyping of Microorganism
PublikacjaTechniques relies on the ligation of appropriates adapters (LM-PCR) as AFLP, PCR MP and ADSRRS are successfully used for epidemiological studies for prokaryotic and eukaryotic microorganisms. In this study we propose a new method, called the LM-PCR/Shifter, based on the use of a Class IIS restriction enzyme giving restriction fragments with different 4 base 5' overhangs (Shifter) and the ligation of appropriate oligonucleotide...
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Evaluation of possibilities of genotyping of Candida glabrata clinical isolates with RAPD-PCR method and microsatellite analysis
PublikacjaThe aim of the study was to compare the discriminatory power of RAPD-PCR method using RSD10 primer and microsatellite (GACA)4 analysis for genotyping clinical isolates of C. glabrata. Isolates were received from patients of two Polish hospitals: Children's Memorial Health Institute in Warsaw (n=17) and Medical University of Gdansk (n=37). Species identification was confirmed by two phenotypic methods: growth on chromagar plates...
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IN VITRO PROPAGATION OF RHODODENDRON TOMENTOSUM - AN ENDANGERED ESSENTIAL OIL BEARING PLANT FROM PEATLAND
PublikacjaRhododendron tomentosum Harmaja (formerly Ledum palustre L.) is a medicinal peat bog plant native to northern Europe, Asia and North America. This plant has a distinctive aroma thanks to the presence of essential oil, to which it also owes its anti-inflammatory, analgesic, antimicrobial and insecticidal properties. However, in Europe R. tomentosum is classified as an endangered species, mainly due to degradation of peatlands. In...
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Performance Analysis of Machine Learning Methods with Class Imbalance Problem in Android Malware Detection
PublikacjaDue to the exponential rise of mobile technology, a slew of new mobile security concerns has surfaced recently. To address the hazards connected with malware, many approaches have been developed. Signature-based detection is the most widely used approach for detecting Android malware. This approach has the disadvantage of being unable to identify unknown malware. As a result of this issue, machine learning (ML) for detecting malware...
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Activation Energy and Inclination Magnetic Dipole Influences on Carreau Nanofluid Flowing via Cylindrical Channel with an Infinite Shearing Rate
PublikacjaThe infinite shear viscosity model of Carreau fluid characterizes the attitude of fluid flow at a very high/very low shear rate. This model has the capacity for interpretation of fluid at both extreme levels, and an inclined magnetic dipole in fluid mechanics has its valuable applications such as magnetic drug engineering, cold treatments to destroy tumors, drug targeting, bio preservation, cryosurgery, astrophysics, reaction kinetics,...
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The voltage on bus bars of the main switchboard of the car carrier electrical power system during sea trials
Dane BadawczeThe dataset is part of the research results on the quality of supply voltage on bus bars of the main switchboard of the ship's electrical power system in different states of ship exploitation. The attached dataset contains the measurement results conducted onboard the car carrier during sea trials.
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The voltage on bus bars of the main switchboard of the car carrier electrical power system at sea trials during maneuvering
Dane BadawczeThe dataset is part of the research results on the quality of supply voltage on bus bars of the main switchboard of the ship's electrical power system in different states of ship exploitation. The attached dataset contains the measurement results conducted onboard the car carrier at sea trials during maneuvering.
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The conducted immunity test of an AC adaptor in accordance with EMC standards
Dane BadawczeThe dataset presents a result of measurements that are a part of immunity tests to conducted disturbances, induced by radio-frequency fields. The immunity tests were carried out on the mains cable of the ac adaptor PHILIPS DC power supply SBC 6654. Tests of immunity of electronic systems to conducted disturbances in the frequency range from 150 kHz...
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UV Light-Modulated Fluctuation-Enhanced Gas Sensing by Layers of Graphene Flakes/TiO2 Nanoparticles
PublikacjaWe present experimental results of fluctuation-enhanced gas sensing by low-cost resistive sensors made of a mixture of graphene flakes and TiO2 nanoparticles. Both components are photocatalytic and activated by UV light. Two UV LEDs of different wavelengths (362 and 394 nm) were applied to modulate the gas sensing of the layers. Resistance noise was recorded at low frequencies, between 8 Hz and 10 kHz. The sensors’ response was...
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Broken rotor bar impact on sensorless control of induction machine
PublikacjaThe aim of the research is analysis of the sensorless control system of induction machine with broken rotor for diagnostic purposes. Increasing popularity of sensorless controlled variable speed drives requires research in area of reliability, range of stable operation, fault symptoms and application of diagnosis methods. T transformation (Cunha et al.,2003) used for conversion of instantaneous rotor currents electrical circuit...
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Genotyping Techniques for Determining the Diversity of Microorganisms
PublikacjaTyping of microbial pathogens, or identifying bacteria at the strain level, is particularly important for diagnosis, treatment, and epidemiological surveillance of bacterial infections. This is especially the case for bacteria exhibiting high levels of antibiotic resistance or virulence, and those involved in nosocomial or pandemic infections. Strain typing also has applications in studying bacterial population dynamics. The part...
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Molecular identification and genotyping of Staphylococci: genus, species, strains, colnes, lineages, and interspecies exchanges
PublikacjaStaphylococci are increasingly recognized as etiological agents of many opportunistic human and animal infections, emphasizing the need for a rapid and accurate identification, even to a genotypical level of these bacteria. In the recent years, there has been a significant progress in typing and phylogenetic study of Staphylococcus species. Here, we describe molecular methods used in taxonomy as well as staphylococci characterization....
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Mathematical modeling and prediction of pit to crack transition under cyclic thermal load using artificial neural network
PublikacjaThe formation of pitting is a major problem in most metals, which is caused by extremely localized corrosion that creates small holes in metal and subsequently, it changes into cracks under mechanical load, thermo-mechanical stress, and corrosion process factors. This research aims to study pit to crack transition phenomenon of steel boiler heat tubes under cyclic thermal load, and mathematical modeling...