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Search results for: SP ECTROSCOPIC METHODS
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Opracowanie dogodnej metody tworzenia wiązań S-C o hybrydyzacji atomu węgla sp oraz sp2
PublicationBadania przeprowadzone w Katedrze Chemii Organicznej Wydziału Chemicznego Politechniki Gdańskiej na przestrzeni ostatnich kilku lat, pokazały wszechstronność pochodnych kwasu neopentylidenofosforoditiowego. Dotychczasowe wyniki badań doprowadziły mnie do opracowania nowych strategii syntetycznych, umożliwiających otrzymywanie związków zawierających wiązanie C-S o hybrydyzacji atomu węgla sp oraz sp2. Analizując wyniki badań opracowałam...
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Characterization of a cryptic plasmid pSFKW33 from Shewanella sp. 33b
PublicationA cryptic plasmid pSFKW33 from psychrotrophic bacterium Shewanella sp. 33B, an isolate from the Gulf of Gdansk (the Baltic Sea), was sequenced and characterized. It is an 8021 bpcircular molecule with 38% GC content, which shows a distinctive nucleotide sequence without homology to other known plasmids. The nucleotide sequence analysis predicts eight open reading frames. The deduced amino acid sequence of ORF-1 shared significant...
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A cold-adapted esterase from psychrotrophic Pseudoalteromonas sp. strain 643A
PublicationDzięki zastosowaniu lipaz możliwa jest biotechnologiczna produkcja m.in. biodegradowalnych biopolimerów, biopaliw (tzw. biodiesel), wielu terapeutyków, herbicydów, pestycydów, kosmetyków, substancji smakowych, serów i in. Szczególnie interesującą grupę stanowią enzymy lipolityczne, pochodzące z organizmów psychrofilnych i psychrotrofowych, ze względu na zdolność do wydajnego katalizowania reakcji w temperaturach rzędu 10-20 ºC....
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Structure of EstA esterase from psychrotrophic Pseudoalteromonas sp. 643A covalently inhibited by monoethylphosphonate.
PublicationThe crystal structure of the esterase EstA from the cold-adapted bacteriumPseudoalteromonas sp. 643A was determined in a covalently inhibited form at aresolution of 1.35 A˚. The enzyme has a typical SGNH hydrolase structureconsisting of a single domain containing a five-stranded beta-sheet, with threehelices at the convex side and two helices at the concave side of the sheet, and isornamented with a couple of very short helices...
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Extracellular secretion of Pseudoalteromonas sp. cold-adapted esterase in Escherichia coli in the presence of Pseudoalteromonas sp. components of ABC transport system
PublicationEnzymy lipolityczne, głównie lipazy i esterazy, stanowią cenną grupę biokatalizatorów wykorzystywanych w przemyśle. Coraz większe zainteresowanie tą klasą enzymów sprawiło, że poszukuje się alternatywnych źródeł ich pozyskiwania. W celu zbadania wpływu białek transportu ABC Pseudoalteromonas sp. 643A na ekspresję genu, sekrecję i aktywność esterazy EstA z tego mikroorganizmu w systemie ekspresyjnym E. coli skonstruowano układ do...
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Molecular characterization of a cryptic plasmid from the psychrotrophic antarctic bacterium Pseudoalteromonas sp. 643A
PublicationW ostatnich latach znacznie wzrosło zainteresowanie organizmami zimnolubnymi, głównie z powodu ich enzymów o biotechnologicznym znaczeniu. Jednak, aby wydajnie produkować takie enzymy w skali przemysłowej użyteczne jest stworzenie rekombinantowych systemów ekspresyjnych, które pozwolą na ich opłacalną produkcję. W osiągnięciu tego celu mogą pomóc odpowiednie wektory, konstruowane na bazie plazmidów pozyskiwanych z bakterii psychrofilnych/psychrotrofowych....
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A new B-D-galactosidase with a low temperature optimum isolated from the Antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization.
PublicationA psychrotrophic bacterium producing a coldadaptedB-galactosidase upon growth at low temperatureswas classiWed as Arthrobacter sp. 20B. A genomic DNAlibrary of strain 20B introduced into Escherichia coliTOP10F' and screening on X-Gal (5-bromo-4-chloro-3-indolyl-B-D-galactopyranoside)-containing agar plates ledto the isolation of B-galactosidase gene. The B-galactosidasegene (bgaS) encoding a protein of 1,053 amino acids,with a...
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Optimization of Polycrystalline CVD Diamond Seeding with the Use of sp³/sp² Raman Band Ratio
PublicationThe influence of various nanodiamond colloids used for seeding nondiamond substrates in microwave plasma enhanced chemical vapour deposition diamond process was investigated. Colloids based on deionized water, isopropanol alcohol and dimethyl sulfoxide (DMSO) were used with different grain size dispersion: 150, 400 and 35 nm, respectively. The influence of growth time was also taken into consideration and bias enhanced nucleation....
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Expression of a GDSL esterase from Pseudomonas sp. S9 in Pichiapastoris
PublicationCold active lipolytic enzymes are promising to replace the conventional enzymes processes of biotechnological industries. One of the most important feature of the cold-active lipases and esterases is that they offer economic benefits through energy saving. In general, they exhibit high activity at low temperatures and low thermostability at moderate temperatures. Lipolytic enzyme EstS9 was isolated from Pseudomonas sp. S9. A multiple...
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Environmental consequences of emulsifying properties of biosurfactant produced by Pseudomonas sp.
PublicationBiosurfactants are natural surfactants produced by variety of microorganisms. They are very prospective product due to good biodegradability, low toxicity, good physicochemical properties and high efficiency in many applications. Moreover, they are efficient in wide range of pH, temperatures and salinity. Thus, they have potential to be used in many applications. However, their later presence in municipal and industrial wastewater...
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Identyfikacja i izolacja genu kodującego zaadaptowaną do zimna β-galaktozydazę Paracoccus sp. = Identification and izolation of the gene encoding cold-active β-galactosidase from paracoccus sp.
PublicationZaprezentowane badania były prowadzone u eehi wyizolowania ze środo¬wiska organizmu psychrofilnego, zdolnego do hydrolizy laktozy w niskiej tempe¬raturze, oraz zidentyfikowania genu kodującego (i-galaktozydazę zaadaptowaną do zimna.Poszukiwan gen został w izolowany z psychrofilnego szczepu autarkicz¬nego Paracoccus sp. 321). Na podstawie sekwencji nukleotydowej genu kodują¬cego fS-galaktozdaze ustalono skład aminokwasów),...
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Beta-Galactosidases of Pseudoalteromonas sp. – Characteristics and application for lactose hydrolysis and galactopyranosides synthesis
PublicationBeta-galactosidase [EC 3.2.1.23] is an enzyme that catalyzes the hydrolysis of O-glycosidic linkages in galactosides. It has biotechnological applications in the dairy industry for the production of milk with a low content of lactose and galactooligosacharides for use in healthy food. The transgalactosylation activity of beta-galactosidase can be used in the synthesis of alkyl galactopyranosides. Due to the fact that conditions of...
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B-GALACTOSIDASE ARTHROBACTER SP. 32cB - OBTAINING THE GENE SEQUENCE, CONSTRUCTION OF THE EXPRESSION SYSTEM, BIOSYNTHESIS AND BIOCHEMICAL CHARACTERIZATION OF THE ENZYME
PublicationINTRODUCTION: β-Galactosidase is an enzyme which catalyzes the hydrolysis of O glycosidic bond in β-galactosides. Another activity of β galactosidase is a transglycosylation activity. The main industrial use of this protein is the hydrolysis of lactose in milk in a cooling conditions. Synthesis of galactooligosaccharides, which are mostly used as a prebiotics added to some foods or available as dietary supplements, is only one...
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A new cold-active β-galactosidase from Arthrobacter sp. S3* - gene cloning, overexpression, purification and properties
PublicationA psychrotrophic bacterium producing a cold-active β-galactosidase was isolated from Spitsbergen soil and classified as Arthrobacter sp. S3*. The gene encoding β-galactosidase was isolated from the genomic DNA library, sequenced, cloned, expressed in Escherichia coli, purified by ion exchange chromatography and characterized. The Arthrobaster sp. S3* β-galactosidase is a homotrimeric enzyme composed of 74,4 kDa subunits. It is...
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Antarctic marine bacterium Pseudoalteromonas sp. 22b as a source of cold adapted á-galaktosidase.
PublicationAntarktyczny morski szczep Pseudoalteromonas sp. 22b. jest źródłem wewnątrzkomórkowej beta-galaktozy. Enzym ten wykazuje wysoką aktywność hydrolizy laktozy w zakresie temperatur od 0 do 20 st. C oraz pH 6,0-8,0. W niniejszej pracy opisano metodykę uzyskania homogennego preparatu tego enzymu, oraz pełną charakterystykę jego własności enzymatycznych i kinetycznych.
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Comparison of the content of phenolic compounds and antioxidant activity of white, red and black currants (Ribes sp.) extracts
PublicationThe aim of this study was to compare the content of antioxidant compounds and the antioxidant activity of extracts from white, red and black currants (Ribes sp.). The chemical properties verified included determinations of anthocyanins and other phenols by HPLC-DAD-MS, total antioxidant activity by standard spectrophotometric tests (ABTS, DPPH and FCR), and profiles of antioxidants by TLC with visualization reagents (ABTS, DPPH,...
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Cloning, expression, and purification of a recombinant cold-adapted β-galactosidase from antarctic bacterium Pseudoalteromonas sp. 22b
PublicationAntarktyczny szczep, gram-ujemnych bakterii Pseudoalteromonas sp. 22b, wyizolowanych z przewodu pokarmowego kryla z gatunku Thyssanoessa macrura, jest producentem wewnątrzkomórkowej zimnolubnej beta-galaktozydazy. Białko to jest homotetramerem, w którym każda z jednostek monomeru zbudowana jest z 1028 reszt aminokwasowych. Gen kodujący ten enzym został sklonowany i eksprymowany w komórkach E. coli. Następnie z zastosowaniem procedury...
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Projekt belki hybrydowej 1b dla firmy H+H Polska sp. z o.o.
PublicationNiniejsze opracowanie stanowi projekt belki nadprożowej o długości 3,0 m o przekroju poprzecznym belki hybrydowej (beton konstrukcyjny łączony z betonem komórkowym) nawiązującej do wymiaru bloczka z betonu komórkowego firmy H+H Polska sp. z o.o. o wymiarach (szerokość × wysokość × długość): 10 × 24 × 60 cm, odmiany D600 o średniej wytrzymałości na ściskanie 3,0 MPa. Całkowita wysokość belki hybrydowej wynosi 24 cm.
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Projekt belki hybrydowej 1a dla firmy H+H Polska sp. z o.o.
PublicationNiniejsze opracowanie stanowi projekt belki nadprożowej o długości 3,0 m o przekroju poprzecznym belki hybrydowej (beton konstrukcyjny łączony z betonem komórkowym) nawiązującej do wymiaru bloczka z betonu komórkowego firmy H+H Polska sp. z o.o. o wymiarach (szerokość × wysokość × długość): 24 × 24 × 60 cm, odmiany D600 o średniej wytrzymałości na ściskanie 3,0 MPa. Całkowita wysokość belki hybrydowej wynosi 27 cm (wraz z 3 cm...
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Antarctic, cold-adapted beta-galactosidase of Pseudoalteromonas sp. 22b as an effective tool for alkyl galactopyranosides synthesis
PublicationPrzedstawione jest zastosowanie arktycznej beta-galaktozydazy Pseudoalteromonas sp. 22b do katalizy syntezy alkilowych galaktopyranozydów. Njwyższą wydajność syntezy uzyskuje się przy pH 6-9 i 10-30% stężenia wody. Podobnie jak dla mezofilnych beta-galaktozydaz enzym antarktyczny syntetyzował najwydajniej kiedy reakcja przebiega w mieszaninie buforu i rozpuszczalnika organiczego (poniżej 50%, v/v).
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THE GENE CLONING, OVEREXPRESSION, PURIFICATION AND BIOCHEMICAL CHARACTERISATION OF A NEW COLD-ADAPTED β–GALACTOSIDASE FROM ARTHROBACTER SP. VII-4
PublicationINTRODUCTION. β-Galactosidase [EC 3.2.1.23] is an enzyme that catalyzes the hydrolysis of O-glycosidic linkages in galactosides. It is commercially used in dairy industry for the production of milk with reduced lactose content. Potentially, the best method for lactose removal under cooling conditions should be carried out with a cold-adapted enzyme. AIM. The aim of this study was to determine the taxonomic affiliation of the isolate...
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Ocena koncepcji estetyzacji dworca Warszawa Centralna w Warszawie (Sud Architekt Polska Sp. z o.o., luty 2014)
PublicationOcena funkcjonalno-przestrzenna koncepcji estetyzacji dworca Warszawa Centralna w Warszawie, wykonanej przez Sud Architekt Polska Sp. z o.o., luty 2014
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A novel cold-active beta-D-galactosidase from the Paracoccus sp. 32d - gene cloning, purification and characterization
PublicationBeta-D-galactosidase (EC 3.2.1.23) catalyze the hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Cold-active beta-D-galactosidases have recently become a focus of attention of researchers and dairy product manufactures owing to theirs ability to: (I) eliminate of lactose from refrigerated milk for people afflicted with lactose intolerance, (II) convert lactose to glucose and galactose which...
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BIOSYNTEZA ESTERAZY Z PSEUDOMONAS SP. S9 W KOMÓRKACH PICHIA PASTORIS, OCZYSZCZANIE I CHARAKTERYSTYKA
PublicationEnzymy lipolityczne ze względu na swoje unikalne właściwości takie jak stereo-, chemo- i regiospecyficzność znajdują szereg zastosowań w różnych gałęziach przemysłu. Lipazy i esterazy wykorzystywane są w przemyśle chemicznym, farmaceutycznym, spożywczym oraz w syntezie organicznej. W ramach wcześniejszych badań zidentyfikowałam i scharakteryzowałam aktywną w niskiej temperaturze esterazę z Pseudomonas sp. S9. Esteraza Pseudomonas...
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Structural studies of a cold-adapted dimeric Beta-D-galactosidase from Paracoccus sp. 32d
PublicationThe crystal structure of a novel dimeric [beta]-D-galactosidase from Paracoccus sp. 32d (Par[beta]DG) was solved in space group P212121 at a resolution of 2.4 Å by molecular replacement with multiple models using the BALBES software. This enzyme belongs to glycoside hydrolase family 2 (GH2), similar to the tetrameric and hexameric [beta]-D-galactosidases from Escherichia coli and Arthrobacter sp. C2-2, respectively. It is the second...
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Review of Segmentation Methods for Coastline Detection in SAR Images
PublicationSynthetic aperture radar (SAR) images acquired by airborne sensors or remote sensing satellites contain the necessary information that can be used to investigate various objects of interest on the surface of the Earth, including coastlines. The coastal zone is of great economic importance and is also very densely populated. The intensive and increasing use of coasts and changes of coastlines motivate researchers to try to assess...
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TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp.,recognizes novel asymmetric sequence 5´-ACGGA(N11/9)-3
PublicationA novel prototype class-IIS restriction endonuclease, TspGWI, was isolated from the thermophilic bacterium Thermus sp. GW. The recognition sequence and cleavage positions have been established: TspGWI recognizes the non-palindromic 5-bp sequence 5′-ACGGA-3′ and cleaves the DNA 11 and 9 nt downstream in the top and bottom strand, respectively. In addition, an accompanying endonuclease, TspGWII, an isoschizomer of Pst I, was found...
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Projekt dwóch belek hybrydowych dla firmy H+H Polska sp. z o.o.
PublicationOpracowanie stanowi projekt dwóch belek nadprożowych o długości 3,0 m każda o przekroju poprzecznym belki hybrydowej (beton konstrukcyjny łączony z betonem komórkowym) nawiązującej do wymiaru bloczka z betonu komórkowego firmy H+H Polska sp. z o.o. o wymiarach (szerokość × wysokość × długość): 24 × 24 × 60 cm, odmiany D600 o średniej wytrzymałości na ściskanie 3,0 MPa (zgodnie z kartą techniczną w załączniku do opracowania). Całkowita...
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Projekt belki hybrydowej 1b dla firmy H+H Polska sp. z o.o. wariant 2
PublicationNiniejsze opracowanie stanowi projekt belki nadprożowej o długości 3,0 m o przekroju poprzecznym belki hybrydowej (beton konstrukcyjny łączony z betonem komórkowym) nawiązującej do wymiaru bloczka z betonu komórkowego firmy H+H Polska sp. z o.o. o wymiarach (szerokość × wysokość × długość): 10 × 24 × 60 cm, odmiany D600 o średniej wytrzymałości na ściskanie 3,0 MPa. Całkowita wysokość belki hybrydowej wynosi 24 cm.
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Projekt belki hybrydowej 1a dla firmy H+H Polska sp. z o.o. wariant 2
PublicationNiniejsze opracowanie stanowi projekt belki nadprożowej o długości 3,0 m o przekroju poprzecznym belki hybrydowej (beton konstrukcyjny łączony z betonem komórkowym) nawiązującej do wymiaru bloczka z betonu komórkowego firmy H+H Polska sp. z o.o. o wymiarach (szerokość × wysokość × długość): 24 × 24 × 60 cm, odmiany D600 o średniej wytrzymałości na ściskanie 3,0 MPa. Całkowita wysokość belki hybrydowej wynosi 27 cm (wraz z 3 cm...
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A novel cold-active β-D-galactosidase with transglycosylation activity from the Antarctic Arthrobacter sp. 32cB - gene cloning, purification and characterization
PublicationA gene encoding a novel β-D-galactosidase from the psychrotolerant Antarctic bacterium Arthrobacter sp. 32cB was isolated, cloned and expressed in Escherichia coli. The active form of recombinant β-D-galactosidase consists of two subunits with a combined molecular weight of approximately 257 kDa. The enzyme's maximum activity towards o-nitrophenyl-β-D-galactopyranoside was determined as occurring at 28 °C and pH 8.0. However, it...
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Fungal Typin Methods
PublicationThe broad application of the molecular techniques in mycoses diagnosis is related to increase of infections caused by fungi in many countries. The oldest typing methods relaying on fenotypic observation, physiological and biochemical examination have had very limited importance from decades. Novadays, the molecular biology methods took their place. Most of the genotyping methods have been devoleped to be applied for typing of bacteria...
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Methods for biomaterials printing: A short review and perspective
PublicationPrinting technologies have opened larger windows of innovation and creativity to biomaterials engineers by providing them with the ability to fabricate complex shapes in a reasonable time, cost, and weight. However, there has always been a trouble with function adjusting in printing technologies in view of the multiplicity of materials and apparatus parameters. 3D printing, also known as additive manufacturing, revolutionized biomaterials...
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In Situ Random Microseeding and Streak Seeding Used for Growth of Crystals of Cold-Adapted beta-D-Galactosidases: Crystal Structure of betaDG from Arthrobacter sp. 32cB
PublicationThere is an increasing demand for cold-adapted enzymes in a wide range of industrial branches. Nevertheless, structural information about them is still scarce. The knowledge of crystal structures is important to understand their mode of action and to design genetically engineered enzymes with enhanced activity. The most difficult task and the limiting step in structural studies of cold-adapted enzymes is their crystallization,...
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Advanced operating methods
PublicationSelected operating methods of resistive gas sensors were presented. The methods utilize flicker noise (1/f noise), which gives additional information about ambient gas when compared with the recorded changes of DC resistance only. Methods of flicker noise measurements were outlined. Recently developed prototype gas sensors comprising golden nanoparticle functionalized with organic ligands can generate intense flicker noise induced...
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Kalanchoe sp. Extracts—Phytochemistry, Cytotoxic, and Antimicrobial Activities
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Dokumentacja oraz wnioski z badań belek hybrydowych dla firmy H+H Polska sp. z o.o.
PublicationOpracowanie stanowi dokumentację oraz wnioski z badań belek nadprożowych o długości 3,0 m każda o przekroju poprzecznym belki hybrydowej (beton konstrukcyjny łączony z betonem komórkowym) nawiązującej do wymiaru bloczka z betonu komórkowego firmy H+H Polska sp. z o.o. o wymiarach (szerokość × wysokość × długość): 24 × 24 × 60 cm, odmiany D600 o średniej wytrzymałości na ściskanie 3,0 MPa (zgodnie z kartą techniczną w załączniku...
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Purification and biochemical characteristic of a cold-active recombinant esterase from Pseudoalteromonas sp. 643A under denaturing conditions.
PublicationIn this paper production of a cold-active esterase EstA from the Antarctic bacterium Pseudoalteromonas sp. 643A in E. coli expression system was described. The purification and biochemical characteristic of EstA were performed in the presence of urea and then compared with results obtained for the esterase with no addition of urea and isolated from the native source. In both cases the cold-active enzyme displayed similar properties....
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Explicit and implicit difefrence methods for quasilinear first order partial functional differential equations.
PublicationInitial boundary value problems of the Dirichlet type for quasilinear functional differential equations are considered. Explicit difference schemes of the Euler type and implicit difference methods are investigated. Suffcient conditions for the convergence of approximate solutions are given and comparisons of the methods are presented. It is proved that assumptions on the regularity of given functions are the same for both classes...
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Modern Methods of Software Development
PublicationSoftware development methods consist of such activities like analysis, planning, development, testing, deployment, maintenance and retirement. All of them can be divided into two main categories - traditional and agile. The objective of this paper is to review some of the most popular traditional, agile and open source development methods. Special attention was paid to the common stages of all methods - testing and maintenance.
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Mapping the Transglycosylation Relevant Sites of Cold-Adapted β-D-Galactosidase from Arthrobacter sp. 32cB
PublicationB-Galactosidase from Arthrobacter sp. 32cB (ArthbetaDG) is a cold-adapted enzyme able to catalyze hydrolysis of beta-D-galactosides and transglycosylation reaction, where galactosyl moiety is being transferred onto an acceptor larger than a water molecule. Mutants of ArthbetaDG D207A and E517Q were designed to determine the significance of specific residues and to enable formation of complexes with lactulose and sucrose and to...
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A new cold-adapted beta-D-galactosidase from the Antarctic Arthrobacter sp. 32c - gene cloning, overexpression, purification and properties
PublicationThe development of a new cold-active β-D-galactosidases and microorganisms that efficiently ferment lactose is of high biotechnological interest, particularly for lactose removal in milk and dairy products at low temperatures and for cheese whey bioremediation processes with simultaneous bio-ethanol production. In this article, we present a new β-D-galactosidase as a candidate to be applied in the above mentioned biotechnological...
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Metody badania interferencji wywoływanych przez prądy błądzące na przykładzie pomiarów wykonanych na terenie Mazowieckiej Spółki Gazownictwa sp. z o.o.
PublicationOmówiono stosowane w praktyce metody badania interferencji wywoływanych przez prądy błądzące na rurociągach podziemnych. Zaprezentowano przykładowe wyniki pomiarów wraz z analizą zagrożenia korozją elektrolityczną, które wykonano podczas diagnostyki stalowej dystrybucyjnej sieci gazowej eksploatowanej przez Mazowiecką Spółkę Gazownictwa sp. z o.o.
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Methods of ZnO nanoparticles synthesis
PublicationZinc oxide nanostructures are interesting nanomaterials with a wide range of applications. Since the physical and chemical properties of ZnO nanoparticles are influenced both by their shape and size, a control of morphology of ZnO structures is needed for their commercial usage. Different chemical, physical, and biological methods used to produce ZnO nanostructures can be found in the literature. The production of ZnO nanoparticles...
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Investigating the Interaction Between Streptomyces sp. and Titania/Silica Nanospheres
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Cloning, expression in Komagataella phaffii, and biochemical characterization of recombinant sequence variants of Pseudomonas sp. S9 GDSL-esterase
PublicationTwo recombinant Komagataella phaffii (formerly Pichia pastoris) yeast strains for production of two sequential variants of EstS9 esterase from psychrotolerant bacterium Pseudomonas sp. S9, i.e. αEstS9N (a two-domain enzyme consisting of a catalytic domain and an autotransporter domain) and αEstS9Δ (a single-domain esterase) were constructed. However, only one of recombinant K. phaffii strains, namely Komagataella phaffii X-33/pPICZαestS9Δ,...
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CE-MS and GC-MS as “Green” and Complementary Methods for the Analysis of Biogenic Amines in Wine
PublicationTwo novel complementary analytical methods, namely an extraction-free capillary electrophoresis coupled to mass spectrometry (CE-MS) and direct immersion-solid-phase microextraction combined with gas chromatography-mass spectrometry (DI-SPME-GC-MS), have been developed and successfully applied for the determination of biogenic amines (BAs) in wine and fruit wine. They have been rigorously compared to each other in terms of various...
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Application of analytical methods for the determination of bioactive compounds in some berries
PublicationFluorometry, ESI-MS, FTIR, and radical scavenging assays were used for characterization of bioactive compounds and the levels of their antioxidant activities. Polyphenols, flavonoids, anthocyanins, and ascorbic acid and the level of antioxidant activity of water extracts of “Murtilla-like” [Myrteola nummularia (Poiret) Berg.], and other widely consumed berries were determined and compared. The contents of bioactive compounds and...
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Review on Wikification methods
PublicationThe paper reviews methods on automatic annotation of texts with Wikipedia entries. The process, called Wikification aims at building references between concepts identified in the text and Wikipedia articles. Wikification finds many applications, especially in text representation, where it enables one to capture the semantic similarity of the documents. Also, it can be considered as automatic tagging of the text. We describe typical...
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Color transformation methods for dichromats
PublicationColor blindness is a serious perception problem. Suffering individuals cannot understand messages, which are carrying by many images, especially those, distributed by WWW. In this paper we are proposing three image processing methods to enhance image recognition and understanding by persons with dichromacy. Color difference image is introduced to represent color perception dissimilarity. Two color transformation methods are presented....