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Search results for: MOTOR PROTEINS
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Delay induced oscillations in gene expression of Hes1 protein model
PublicationW pracy został omówiony model produkcji białka Hes1. Stężenie Hes1 jest kontrolowane przez ujemne sprzężenie zwrotne wbudowane w białko i jego mRNA. Przeprowadzona analiza potwierdziła znane wyniki numeryczne, że oscylacje wywołane są przez opóźnienie. Pokazaliśmy również, że jeśli współczynnik Hilla jest większy niż 1, to istnieje krytyczna wartość opóźnienia, dla której ma miejsce bifurkacja Hopfa i pojawiają się rozwiązania...
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MP3vec: A Reusable Machine-Constructed Feature Representation for Protein Sequences
Publication—Machine Learning (ML) methods have been used with varying degrees of success on protein prediction tasks, with two inherent limitations. First, prediction performance often depends upon the features extracted from the proteins. Second, experimental data may be insufficient to construct reliable ML models. Here we introduce MP3vec, a transferable representation for protein sequences that is designed to be used specifically for sequence-to-sequence...
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X-ray and UV Radiation Damage of dsDNA/Protein Complexes
PublicationRadiation and photodynamic therapies are used for cancer treatment by targeting DNA. However, efficiency is limited due to physico-chemical processes and the insensitivity of native nucleobases to damage. Thus, incorporation of radio- and photosensitizers into these therapies should increase both efficacy and the yield of DNA damage. To date, studies of sensitization processes have been performed on simple model systems, e.g.,...
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Prediction of protein assemblies, the next frontier: The CASP14‐CAPRI experiment
PublicationWe present the results for CAPRI Round 50, the 4th joint CASP-CAPRI protein assembly prediction challenge. The Round comprised a total of 12 targets, including 6 dimers, 3 trimers, and 3 higher-order oligomers. Four of these were easy targets, for which good structural templates were available either for the full assembly, or for the main interfaces (of the higher-order oligomers). Eight were difficult targets for which only distantly...
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Molecular targets for antifungals in amino acid and protein biosynthetic pathways
PublicationFungi cause death of over 1.5 million people every year, while cutaneous mycoses are among the most common infections in the world. Mycoses vary greatly in severity, there are long-term skin (ringworm), nail or hair infections (tinea capitis), recurrent like vaginal candidiasis or severe, life-threatening systemic, multiorgan infections. In the last few years, increasing importance is attached to the health and economic problems...
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Mechanism of antifreeze protein functioning and the “anchored clathrate water” concept
PublicationIn liquid water, there is a natural tendency to form aggregates that consist of water molecules linked by hydrogen bonds. Such spontaneously formed aggregates are surrounded by a "sea" of disordered water molecules, with both forms remaining in equilibrium. The process of creating water aggregates also takes place in the solvation water of proteins, but in this case the interactions of water molecules with the protein surface shift...
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The Unfolded Protein Response: A Double-Edged Sword for Brain Health
PublicationEfficient brain function requires as much as 20% of the total oxygen intake to support normal neuronal cell function. This level of oxygen usage, however, leads to the generation of free radicals, and thus can lead to oxidative stress and potentially to age-related cognitive decay and even neurodegenerative diseases. The regulation of this system requires a complex monitoring network to maintain proper oxygen homeostasis. Furthermore,...
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Open-Phase Fault Detection Method for Sensorless Five-Phase Induction Motor Drives with an Inverter Output Filter
PublicationThe paper presents the sensorless five-phase induction motor drive implementation with an inverter output filter with third harmonic injection. For the sensorless operation, the required observers are presented for the first and third harmonics with consideration of the inverter output filter. Moreover, the interaction of the observers and the control system is capable to handle a deactivation of one or two phases, which improves...
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A Comparative Study of Fuzzy SMC with Adaptive Fuzzy PID for Sensorless Speed Control of Six-Phase Induction Motor
PublicationMulti-phase motors have recently replaced three-phase induction motors in a variety of applications due to the numerous benefits they provide, and the absence of speed sensors promotes induction motors with variable speed drives. Sensorless speed control minimizes unnecessary speed encoder cost, reduces maintenance, and improves the motor drive’s reliability. The performance comparison of the fuzzy sliding mode controller (FSMC)...
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Analysis of Cogging Torque Reduction Method Effectiveness on the Example of a Surface Mounted Permanent Magnet Synchronous Motor Model
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A Comparative Study of Fuzzy SMC with Adaptive Fuzzy PID for Sensorless Speed Control of Six-Phase Induction Motor
PublicationMulti-phase motors have recently replaced three-phase induction motors in a variety of applications due to the numerous benefits they provide, and the absence of speed sensors promotes induction motors with variable speed drives. Sensorless speed control minimizes unnecessary speed encoder cost, reduces maintenance, and improves the motor drive’s reliability. The performance comparison of the fuzzy sliding mode controller (FSMC)...
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Amides as models to study the hydration of proteins and peptides — spectroscopic and theoretical approach on hydration in various temperatures
PublicationInteractions with water are one of the key factors which determine protein stability and activity in aqueous solutions. However, the protein hydration is still insufficiently understood. N-methylacetamide (NMA) is regarded as a minimal part of the peptide backbone and the relative simplicity of its structure makes it a good model for studies on protein–water interactions. In this paper, the influence of NMA and N,N-dimethylacetamide...
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Protective Effect of Black Tea Against Ethanol-Induced Oxidative Modifications of Liver Proteins and Lipids
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Discrimination of normal oral mucosa from oral cancer by mass spectrometry imaging of proteins and lipids
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Bacterial SSB-like proteins of family Deinococcaceae - indentification, isolation, obtaining, purification and molecular characteristic
PublicationWiększość poznanych białek SSB jest aktywna w formie homotetramerycznej. Od 2002 roku znane są białka organizmów ekstremofilnych. Scharakteryzowano białka mikroorganizmów rodziny Deinococaceae. Uzyskane termostabilne białka stanowią atrakcyjną alternatywę w stosunku do znanych białek SSB w metodach diagnostyki molekularnej, technikach biologii molekularnej i analityce.
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Two highly thermostable paralogous single-stranded DNA-binding proteins from Thermoanaerobacter tengcongensis
PublicationThe thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. coli overexpression systems. The TteSSB2 and TteSSB3 consist of 153 and 150 amino acids with a calculated molecular...
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Efficient removal of dyes and proteins by nitrogen-doped porous graphene blended polyethersulfone nanocomposite membranes
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Investigations of changes in the arabinogalactan proteins (AGPs) structure, size and composition during the fruit ripening process
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Ovocystatin Induced Changes in Expression of Alzheimer’s Disease Relevant Proteins in APP/PS1 Transgenic Mice
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Identification and properties of the Deinococcus grandis andDeinococcus proteolyticus single-stranded DNA binding proteins (SSB)
PublicationW celu poznania właściwości biochemicznych białek SSB pochodzących z Deinococcus grandis (DgrSSB) i Deinococcus proteolyticus (DprSSB) sklonowano zaamplifikowane w reakcji PCR geny ssb w systemie ekspresyjnym Escherichia coli. Geny składają się z 891(DgrSSB) i 876 (DprSSB) nukleotydów kodujących odpowiednio 296 i 291 reszt aminokwasowych o teoretycznie wyznaczonej masie cząsteczkowej dla białek, równej 32,29 i 31,33 kDa. Sekwencja...
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Extracellular Matrix Proteins Expression Profiling in Chemoresistant Variants of the A2780 Ovarian Cancer Cell Line
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Prediction of the structures of proteins with the UNRES force field, including dynamic formation and breaking of disulfide bonds
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Human sperm proteins identified by 2-dimensional electrophoresis and mass spectrometry and their relevance to a transcriptomic analysis
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The Immunogenic and Immunoprotective Activities of Recombinant Chimeric T. gondii Proteins Containing AMA1 Antigen Fragments
PublicationToxoplasmosis, one of the most common parasitoses worldwide, is potentially dangerous for individuals with a weakened immune system, but specific immunoprophylaxis intended for humans is still lacking. Thus, efforts have been made to create an efficient universal vaccine for both animals and humans to overcome the shortcomings of currently used treatment methods and protect all hosts against toxoplasmosis. The current work represents...
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Unraveling the Interplay between DNA and Proteins: A Computational Exploration of Sequence and Structure-Specific Recognition Mechanisms
PublicationMy PhD dissertation focused on DNA-protein interactions and the recognition of specific DNA sequences and structures. I discovered that acidic amino acid residues (Asp/Glu) play a crucial role by exhibiting a preference for cytosine. Their contribution to binding affinity depends on nearby cytosines, balancing electrostatic repulsion with specific interactions. Acidic residues act as negative selectors, discouraging non-cytosine...
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Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus-expression and purification
PublicationSklonowano w system ekspresyjny pET-30LIC Escherichia coli gen kodujący białka SSB-podobne Thermus thermophilus i T. aquaticus. Oczyszczono do homogenności białka SSB-podobne, a uzyskane preparaty umożliwiają wykonanie badań strukturalnych w/w białek.
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Relationship between seawater pollution and qualitative changes in the extracted proteins from mussels Mytilus galloprovincialis
PublicationCelem badań było znalezienie wiarygodnego bioznacznika dla oceny poziomu zanieczyszczenia wody morskiej. W tym celu porównano:-zawartość żelaza (Fe) oraz miedzi (Cu)-białek-poziomu aktywności przeciwutleniającejmałży (Mytilus galloprovincialis)zebranych z rejonów zanieczyszczonych i niezanieczyszczonych bułgarskiej strefy ekonomicznej Morza Czarnego.W badanich wykorzystano takie techniki analityczne jak: -spektroskopia w podczerwieni...
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The biological role of prolyl oligopeptidase and the procognitive potential of its peptidic inhibitors from food proteins
PublicationProlyl oligopeptidase (POP) is a conserved serine protease belonging to proline-specific peptidases. It has both enzymatic and non-enzymatic activity and is involved in numerous biological processes in the human body, playing a role in e.g., cellular growth and differentiation, inflammation, as well as the development of some neurodegenerative and neuropsychiatric disorders. This article describes the physiological and pathological...
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Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus - expression and purification
PublicationBiałka SSB są niezbędne do życia każdemu organizmowi. W tej pracy prezentujemy wyniki otrzymania i oczyszczania białek SSB Thermus thermophilus i T. aquaticus. Dotąd były znane białka SSB jedynie o budowie homotetrameru lub heterotrimeru. Białka SSB Thermus thermophilus i T. aquaticus stanowią nową klasę białek SSB - białek homodimerycznych. Ponadto dowiedziono, iż białka te są użyteczne w reakcji PCR.
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Impact of rotor geometry optimization on the off-design ORC turbine performance
PublicationThe paper describes the method of CFD based Nelder-Mead optimization of a 10 kW single-stage axial turbine operating in an ORC system working on R7100. The total-to-static isentropic efficiency is defined as an objective function. Multi-point linear regression is carried out to determine the significance of the objective function arguments and to pick up the set of particular variables and characteristic quantities (e.g. flow angles)...
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Artificial-Neural-Network-Based Sensorless Nonlinear Control of Induction Motors
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IPMSM rotor position estimator based on analysis of phase current derivatives
PublicationThis paper describes an algorithm for estimation of IPMSM angular rotor position. The algorithm uses derivatives of motor phase currents resulting from PWM modulation to obtain the rotor position. The presented method is designed for medium- and high-speed range, since it is based on determination of the EMF vector. Algorithm is characterised by a very simple formulae. The calculation of rotor position is performed in every PWM...
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Properties of sensorless control systems based on multiscalar models of the induction motors
PublicationCelem artykułu jest opracowanie ogólnych postaci multiskalarnych modeli silnika indukcyjnego i zaprezentowanie właściwości bezczujnikowych układów regulacji opartych na takich modelach. Poprzednio prezentowane modele multiskalarne silnika indukcyjnego oparte na wykorzystaniu wektorów prądu stojana i strumienia wirnika zostły uogólnione jako model typu 1. Nowy model typu 2 zdefiniowany został dla wektora prądu stojana i innego wektora...
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Slot harmonics in cage motors due to saturation of a main magnetic circuit
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Thermal failure of a second rotor stage in heavy duty gas turbine
PublicationThe impulse mode of operation and the supply of various types of fuels causes frequent failures even in the heavy duty gas turbines. The paper presents the ravages of second rotor stage failure in a gas turbine. The excessive thermal elongation rise caused by fuel change was indicated as the main cause. We applied nonlinear numerical analysis, preceded by thermodynamic calculations of the turbine and visual inspection of the effects...
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An Innovative Floating System with a Savonius Rotor as a Horizontal-Axis Wind Turbine
PublicationIn this project, an innovative wind turbine was designed for a floating plant. A large Savonius rotor was replaced with a double-rotor wind turbine implemented as a horizontal-axis turbine. This double rotor was positioned on the tip of a thrust plate and fixed to the deck of a catamaran. Simple 2D numerical simulations were performed to confirm the effectiveness of the concept. An analysis of the floating system configuration...
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Rotor-Flux Vector based Observer of Interior Permanent Synchronous Machine
PublicationThe sensorless control system of the interior permanent magnet machine is considered in this paper. The control system is based on classical linear controllers. In the machine, there occurs non-sinusoidal distribution of rotor flux together with the slot harmonics, which are treated as the control system disturbances. In this case, the classical observer structure in the (d-q) is unstable for the low range of rotor speed resulting...
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Artificial Neural Network-Based Sensorless Nonlinear Control Of Induction Motors
PublicationW niniejszym artykule przedstawiono strukturę sztucznej sieci neuronowej służącej do korygowania działania układu estymacji prędkości kątowej wirnika. Odtworzona prędkość kątowa wirnika zostały wykorzystane w bezczujnikowym układzie sterowania silnikiem indukcyjnym pracującym w zamkniętej pętli sprzężenia prędkościowego.Przedstawiono wyniki badań eksperymentalnych z silnikiem o mocy 1,1kW.
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Protein overproduction alters exosome secretion in Chinese hamster ovary cells
PublicationDespite the abundance of available cell lines, nearly 70% of all recombinant therapeutic proteins today are produced in Chi-nese hamster ovary (CHO) cells. The impact of protein overproduction on the secretion of exosomes by CHO cells has been investigated here. Increased secretion of extracellular vesicles (EVs) by protein overexpressing CHO cells was demonstrated with protein content assay, nanoparticle tracking analysis, and...
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The impact of training and neurotrophins on functional recovery after spinal cord transection: cellular and molecular mechanisms contributing to motor improvement
PublicationBeneficial effects of locomotor training on the functional recovery after complete transection of the spinal cord indicate that in chronic spinal animals spontaneous recovery processes are enhanced and shaped by the training. The mechanisms of that use-dependent improvement are still not fully understood. This review tackles three aspects of this issue: (1) neurochemical attributes of functional improvement...
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Switching Regulation in the Control of 5-Phase Permanent Magnet Synchronous Motor Fed by 3×5 Direct Matrix Converter
PublicationMatrix converter is an AC-AC direct power converter comprising of an array of bi- directional switches. It does not require an intermediate DC-link and allows sinusoidal output waveforms with varying amplitudes and frequencies. The configuration of these bi- directional switches decides the number of inputs and outputs...
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Thermal analysis and experimental verification of permanent magnet synchronous motor by combining lumped-parameter thermal networks with analytical method
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Core loss resistance impact on sensorless speed control of an induction motor using hybrid adaptive sliding mode observer
PublicationInduction motors (IMs) experience power losses when a portion of the input power is converted to heat instead of driving the load. The combined effect of copper losses, core losses, and mechanical losses results in IM power losses. Unfortunately, the core losses in the motor, which have a considerable impact on its energy efficiency, are not taken into account by the generally employed dynamic model in the majority of the studies. Due...
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A coarse‐grained approach to NMR ‐data‐assisted modeling of protein structures
PublicationThe ESCASA algorithm for analytical estimation of proton positions from coarse-grained geometry developed in our recent work has been implemented in modeling protein structures with the highly coarse-grained UNRES model of polypeptide chains (two sites per residue) and nuclear magnetic resonance (NMR) data. A penalty function with the shape of intersecting gorges was applied to treat ambiguous distance restraints, which automatically...
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CAVER 3.0: A Tool for the Analysis of Transport Pathways in Dynamic Protein Structures
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BALCONY: an R package for MSA and functional compartments of protein variability analysis
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Myotilin, a New Topotecan Resistant Protein in Ovarian Cancer Cell Lines
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C-reactive protein as a diagnostic and prognostic factor in inflammatory bowel diseases
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The Nutritional Value and Biological Activity of Concentrated Protein Fraction of Potato Juice
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Serum Fatty Acid-Binding Protein 4 is Increased in Patients with Psoriasis
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