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Search results for: IN-SITU BIOSENSORS
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A Highly Selective Biosensor Based on Peptide Directly Derived from the HarmOBP7 Aldehyde Binding Site
PublicationThis paper presents the results of research on determining the optimal length of a peptide chain to eectively bind octanal molecules. Peptides that map the aldehyde binding site in HarmOBP7 were immobilized on piezoelectric transducers. Based on computational studies, four Odorant Binding Protein-derived Peptides (OBPPs) with dierent sequences were selected. Molecular modelling results of ligand docking with selected peptides were...
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Capillary waveguide biosensor
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Diamond-based protective layer for optical biosensors
PublicationOptical biosensors have become a powerful alternative to the conventional ways of measurement owing to their great properties, such as high sensitivity, high dynamic range, cost effectiveness and small size. Choice of an optical biosensor's materials is an important factor and impacts the quality of the obtained spectra. Examined biological objects are placed on a cover layer which may react with samples in a chemical, biological...
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Electronic Circuits for Graphene-Based Biosensor
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In situ soil mixing.
PublicationOpracowanie stanowi 9-ty rozdział w książce ''Ground Improvement'' (Wzmacnianie gruntu).W podrozdziałach 9.1 i 9.2 przedstawiono rys historyczny oraz aktualny stan zaawansowania technologii mieszania gruntu na sucho i na mokro. Wprowadzono także klasyfikację metod wdrożonych na świecie przez firmy specjalistyczne. W podrozdziale 9.3 opisano sprzęt i technologię i przebieg mieszania, z podziałem na urządzenia do mieszania głębokiego...
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In-situ and ex-situ resistance measurements of polypyrrole film using double-band electrode
PublicationMany in-situ techniques are performed in order to determine the resistance of conducting polymer film. However, the resistance measured in solution can be the combination of polymer resistance and that of other components, such as resistance of supporting electrolyte. Therefore, in this work, the influence of the solution on the resistance of polypyrrole (PPy) film has been studied. PPy film was electrchemically synthesized onto...
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Low-Coherence Interferometric Fiber-Optic Sensors with Potential Applications as Biosensors
PublicationFiber-optic Fabry-Pérot interferometers (FPI) can be applied as optical sensors, and excellent measurement sensitivity can be obtained by fine-tuning the interferometer design. In this work, we evaluate the ability of selected dielectric thin films to optimize the reflectivity of the Fabry-Pérot cavity. The spectral reflectance and transmittance of dielectric films made of titanium dioxide (TiO2) and aluminum oxide (Al2O3) with...
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Low-Coherence Interferometric Fiber-Optic Sensors with Potential Applications as Biosensors
PublicationFiber-optic Fabry-Pérot interferometers (FPI) can be applied as optical sensors, and excellent measurement sensitivity can be obtained by fine-tuning the interferometer design. In this work, we evaluate the ability of selected dielectric thin films to optimize the reflectivity of the Fabry-Pérot cavity. The spectral reflectance and transmittance of dielectric films made of titanium dioxide (TiO2) and aluminum oxide (Al2O3) with...
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Olfactory receptor-based biosensors as potential future tools in medical diagnosis
PublicationThe detection of biomarkers is the future of non-invasive medical diagnosis and screening. Discovery and identification of reliable disease specific volatile organic compounds is dependent on repeatable, accurate analysis of trace level gaseous analytes mainly in breath samples. Natural variety of the olfactory systems and the compounds capable of gas molecules binding creates wide possibilities of acquisition and implementation...
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Sulfonated poly(benzoyl paraphenylene) as a membrane for PEMFC: Ex situ and in situ experiments of thermal and chemical stability
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Ground improvement with in situ Soil Mixing
PublicationOmówiono metodę wzmacniania słabego gruntu za pomocą technologii wgłębnego mieszania na sucho i mokro. W metodzie mokrej stosuje się składniki wiążące (głownie cementy) wymieszane z wodą, podawane w formie zaczynu. W metodzie suchej materiały wiążące podawane są w postaci sproszkowanej z udziałem sprężonego powietrza. Przedstawiono główne obszary zastosowania w geotechnice oraz podano przykłady kilku realizacji.
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Influence of Thermal Modification and Morphology of TiO2 Nanotubes on Their Electrochemical Properties for Biosensors Applications
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Chiral-based optical and electrochemical biosensors: Synthesis, classification, mechanisms, nanostructures, and applications
PublicationThis review seeks to collect, summarize, classify and discuss the latest advances in chiral-based optical biosensors. Starting from the identification of chiral molecules, photoluminescence, and electrochemical sensors, applications of chiral structures in biosensing molecules are reviewed. Then, biosensors working on the basis of chirality are classified, followed by summarizing the outcomes of research works on design, synthesis,...
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Terahertz Dual-Band Metamaterial Biosensor for Cervical-Cancer Diagnostics
PublicationThis study highlights the potential of employing terahertz metamaterial structures as dual-band biosensors for the early detection of cancerous biological tissue. The fundamental principle leveraged here is the alteration of the effective dielectric constant of biological tissue by cancerous cells. The change in the dielectric constant, in turn, induces a shift in the resonance frequency of the metamaterial sensor. One notable...
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Evaluation of Linkers’ Influence on Peptide-Based Piezoelectric Biosensors’ Sensitivity to Aldehydes in the Gas Phase
PublicationRecent findings qualified aldehydes as potential biomarkers for disease diagnosis. One of the possibilities is to use electrochemical biosensors in point-of-care (PoC), but these need further development to overcome some limitations. Currently, the primary goal is to enhance their metrological parameters in terms of sensitivity and selectivity. Previous findings indicate that peptide OBPP4 (KLLFDSLTDLKKKMSEC-NH2) is a promising...
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Development and Assessment of Regeneration Methods for Peptide-Based QCM Biosensors in VOCs Analysis Applications
PublicationCleaning a quartz crystal microbalance (QCM) plays a crucial role in the regeneration of its biosensors for reuse. Imprecise removal of a receptor layer from a transducer’s surface can lead to unsteady operation during measurements. This article compares three approaches to regeneration of the piezoelectric transducers using the electrochemical, oxygen plasma and Piranha solution methods. Optimization of the cleaning method allowed...
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Functional fluorine-doped tin oxide coating for opto-electrochemical label-free biosensors
PublicationSensors operating in multiple domains, such as optical and electrochemical, offer properties making biosensing more effective than those working in a single domain. To combine such domains in one sensing device, materials offering a certain set of properties are required. Fluorine-doped tin oxide (FTO) thin film is discussed in this work as functional optically for guiding lossy modes and simultaneously electrochemically, i.e....
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Wzmacnianie gruntu in situ metodą wgłębnego mieszania.
PublicationOpisano technologie mieszania wgłębnego na sucho i mokro. Przedstawiono przykłady zastosowania przy budowie dróg w Polsce na słabym podłożu.
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Ground improvement with in-situ wet soil mixing
PublicationOpisano rozwój technologii mieszania wgłębnego gruntu na świecie i w Polsce. Przedstawiono bliżej aktualnie wykorzystywane metody mieszania, wprowadzane przez specjalistyczne firmy. W syntetyczny sposób podsumowano zasady projektowania oraz doświadczenia wykonawcze. Zamieszczono bliższe informacje odnośnie kilku przykładów zastosowania w Polsce.
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In-Situ Processing of Biocomposites via Reactive Extrusion
PublicationLaw regulations, economic and environmental factors are the main causes for the rapidly growing interests in biocomposites’ research, conducted currently in a number of academic and industrial scientific centers. However, weak polymer matrix/filler interactions, common in biocomposites, result in unsatisfactory mechanical properties, which limit their practical applications. From many attempts performed to solve this problem, in-situ...
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Oxygen biosensor based on carbon nanotubes directly grown on graphitic substrate
PublicationFilms of carbon nanotubes were synthesized on the graphitic substrates by CVD method in the presence of catalyst. Two different catalysts were used, that is nickel and iron, supported on graphitic substrates. The as-obtained nanotubes were analyzed by SEM imaging with EDX and Raman spectroscopy. The results revealed their multi-walled morphology. Free radical reaction was carried out in order to chemically functionalize synthesized...
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In situ thermo-optical studies of polymer:fullerene blend films
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Modelowanie konstrukcji mostowych - obliczenia i weryfikacja ''in situ''
PublicationObiekty mostowe są konstrukcjami inżynierskimi, które ze względu na ich odpowiedzialność i długi okres eksploatacji - przed oddaniem do użytkowania poddawane są wszechstronnej ocenie w formie badań, zwanych potocznie próbnym obciążeniem. Zadaniem tych badań jest sprawdzenie poprawności pracy konstrukcji i przyjętych założeń projektowych. Przyciągające uwagę społeczeństwa próbne obciążenie na moście poprzedza projekt jego badań....
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In situ observations of fullerene fusion and ejection in carbon nanotubes
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In Situ Verification of Numerical Model of Water Hammer in Slurries
PublicationThis paper presents a numerical model of transient flow in a pressure slurry pipeline network with verification based on in situ measurements. The model, primarily verified in laboratory conditions, has been extended and applied to the case of a large and complex slurry pipeline network in Poland. In the model, the equivalent density concept was applied. In situ experiments were performed for various unsteady flow episodes, caused by...
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Przenośne urządzenia analityczne do przeprowadzania pomiarów in-situ
PublicationZe względu na złożoność procedury analitycznej, jej czasochłonność oraz koszty jakie należy ponieść w celu oznaczania zanieczyszczeń obecnych w powietrzu atmosferycznym, zaczęto interesować się analizą zanieczyszczeń powietrza, wykonywaną w konkretnym miejscu lub na danym obszarze. Dlatego coraz powszechniejsze stają się urządzenia, które umożliwiają przeprowadzenie analizy w danym miejscu (in situ) bez konieczności transportowania...
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Otrzymywanie kompozytów in situ Al-Si-Fe-Cu
PublicationPrzedstawiono metodykę otrzymywania kompozytów metalowych przez mieszanie mechaniczne ciekłego Al-Si z proszkami Fe i Cu, dzięki samorzutnej reakcji zachodzącej in situ w roztopionym stopie. Przeprowadzono badania budowy kompozytów za pomocą mikroskopu SEM. Wykazano, że fazą wzmacniającą w kompozycie jest Al4SiFe (Vf=15-35%) o twardości 250-350 HV(0,02). Rozmiar i udział objętościowy fazy zbrojącej uzależniony był od sposobu odlewania...
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Microsphere structure application for supercapacitor in situ temperature monitoring
PublicationConstant, real-time temperature monitoring of the supercapacitors for efficient energy usage is in high demand and seems to be crucial for further development of those elements. A fiber-optic sensor can be an effective optoelectronic device dedicated for in-situ temperature monitoring of supercapacitors. In this work, the application of the fiber-optic microstrucutre with thin zinc oxide (ZnO) coating fabricated in the atomic layer...
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Effect of the ex situ physical and in situ chemical modification of bacterial nanocellulose on mechanical properties in the context of its potential applications in heart valve design
PublicationBacterial nanocellulose (BNC) is a promising material for heart valve prostheses. However, its low strength properties limit its applicability in cardiovascular surgery. To overcome these limitations, the mechanical properties of BNC can be improved through modifications. The aim of the research was to investigate the extent to which the mechanical properties of BNC can be altered by modifying its structure during its production...
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Novel electrogravimetric biosensors for the ultrasensitive detection of plasma matrix metalloproteinase-2 considered a potential tumor biomarker
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Fluoroaromatic substituents attached to carbon nanotubes help to increase oxygen concentration on biocathode in biosensors and biofuel cells
PublicationBased on the known ability of perfluorodecalin/perfluorohydrocarbons to enhance oxygen solubility we modified oxygen sensitive biocathode by adding perfluorinated components to the catholite. This procedure improved the efficiency of the oxygen sensitive cathodes. Glassy carbon electrodes covered with single-wall carbon nanotubes (SWCNTs) with covalently bonded perfluoroaromatic groups were shown to be more sensitive to oxygen,...
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New approach in SARS-CoV-2 surveillance using biosensor technology: a review
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New approach in SARS-CoV-2 surveillance using biosensor technology: a review
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An electrochemical biosensor for the determination of hormone Human Chorionic Gonadotropin (hCG) in human serum
PublicationThis work describes the modification of a gold electrode to create an electrochemical biosensor capable of detecting human chorionic gonadotropin (hCG). The biosensor was obtained by modifying the gold electrode with cysteamine and oligopeptide (PPLRINRHILTR). The modification steps of the gold electrode were confirmed by cyclic voltammetry (CV) and impedance electrochemical spectroscopy (EIS) measurements. The conducted EIS experiments...
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High-performance graphene-based biosensor using a metasurface of asymmetric silicon disks
PublicationIn recent years, optical biosensors widely applicable for medical applications, have received much attention. In this paper, we propose a high-performance polarization-insensitive optical biosensor based on a graphene-dielectric metasurface. The metasurface consists of an asymmetric dielectric disk array that supports a high Q-factor Fano resonance. The provided sharp Fano resonance results in an enhanced light-matter interaction...
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THE CIRCUS SITE
PublicationThe location of project is a site of over 1,2 ha, situated on the southern fringes of Gdynia formerly used as an area to host a Circus. Here artists were performing for the public, newcomers were meeting the locals, the temporality of the event was enhancing the experience of exchange. Circus used to be the place where people live, work and create art. Currently the site stays empty but surely not for long. Its potential, attractive...
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Design and simulation of excitation laser system for in-situ Raman monitoring
PublicationPraca opisuje projekt oraz kontrukcję laserowego pobudzania próbek cienkowarstwowych. Zaprezentowana modelowanie optyczne systemu pobudzania oraz konfigurację prototypu. Układ znajdzie zastosowanie w długoogniskowym spektroskopie ramanowskim pracującym na stanowisku PA CVD.
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Electrospun Nanofiber Electrodes with in situ Exsolved Nanocatalysts for Symmetrical SOCs
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Electrospun Nanofiber Electrodes with in situ Exsolved Nanocatalysts for Symmetrical SOCs
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Numerical analysis and in situ tests of the Grot Rowecki Bridge in Warsaw
PublicationThe paper presents the FEM analysis of the reconstructed the Grot-Rowecki Bridge over Vistula river in Warsaw. The bridge has seven spans and consists of two independent structures with the longest spans of 120 m. After reconstruction the bridge is over 10 m wider. The numerical, nonlinear analysis has been con-ducted on a global and local FEM models. These models are defined so as they strictly comply with global structural bridge...
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In-situ optical diagnostics of boron-doped diamond films growth
PublicationInterferometry is a desirable method for in-situ measurement of thin, dielectric film growth, as it don't modify conditions of film deposition. Here we present interferometrical measurements of thickness of doped diamond films during Chemical Vapor Deposition (CVD) process. For this purpose we used a semiconductor laser with a 405nm wavelength. Additional ex-situ measurement using spectral interferometry and ellipsometry...
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Detection of sulfonamide resistance genes via in situ PCR-FISH
PublicationDue to the rising use of antibiotics and as a consequence of their concentration in the environment an increasing number of antibiotic resistant bacteria is observed. The phenomenon has a hazardous impact on human and animal life. Sulfamethoxazole is one of the sulfonamides commonly detected in surface waters and soil. The aim of the study was to detect sulfamethoxazole resistance genes in activated sludge biocenosis by use of...
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Triple correlation states between in-situ tested soil parameters
PublicationWhen testing soil parameters, the measured parameter values are only approximations of the true values. The measurand is determined based on metrological uncertainties or using statistical models for analysing data. Some parameters of the soil state present strong correlations, but others do not always provide simple correspondences. Multiple correlations between geotechnical parameters can provide a new perspective regarding the...
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Mobilne urządzenia analityczne przeznaczone do przeprowadzania pomiarów in-situ
PublicationAnaliza jakości powietrza atmosferycznego wykonywana w danym miejscu (in-situ) z wykorzystaniem mobilnych urządzeń analitycznych staje się coraz częściej praktyką analityczną. W artykule zamieszczono informacje na temat urządzeń wchodzących w skład wyposażenia mobilnych laboratoriów wykorzystywanych do monitorowania i oceny jakości powietrza atmosferycznego. Ponadto zamieszczono przegląd przenośnych chromatografów gazowych oraz...
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An in situ technique for the assessment of adhesive properties of a joint under load
PublicationSlow crack propagation in adhesive bonded joints has been characterised using an asymmetric wedge test. Crack position was evaluated from strain gauge measurements, both in the debonded partof the joint and in the bonded zone. Test temperature was changed during loading, giving insight into bond evolution. The technique allows accurate, and virtually continuous, determination of crack position to be made, and therefore the evaluation...
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Processing and properties of Al-Si-Fe-Cr in situ composites
PublicationPraca jest kontynuacją badań nad wytwarzaniem tanich kompozytów in situ o osnowie stopu Al-Si wzmocnionego związkami międzymetalicznymi Al-Fe-Si lub Al-Fe-Cr-Si, otrzymanych poprzez reakcję ciekłego stopu Al (Al-Si11, Al-Si25) z proszkiem Fe lub Cr. Żelazo zostało wybrane ze względu na niską cenę, niską temperaturę wytwarzania, wysoką reaktywność z Al i heksagonalną sieć krystalograficzną związku Al-Fe-Si, co sprzyja tworzeniu...
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Właściwości kompozytów Al-Si-Fe-Cu otrzymanych in situ
PublicationPrzeprowadzono badania wybranych właściwości kompozytów o osnowie Al-Si z wydzieleniami fazy AlSi4Fe utworzonej in situ przez mieszanie proszków Fe i Cu z ciekłym stopem Al. Zbadano zachowanie materiału przy ściskaniu, twardość oraz zużycie ścierne w warunkach tarcia suchego o płytkę stalową poruszającą się ruchem posuwisto zwrotnym. Przeanalizowano obrazy SEM powierzchni tarcia oraz przekrojów, w celu określenia mechanizmu zużycia...
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Electrooxidation of dissolved dsDNA backed by in situ UV-Vis spectroscopy
PublicationPrzeprowadzono badania elektrochemicznego utleniania dsDNA stosując metody cyklowoltametryczne na elektrodzie weglowej, platynowej, platynowej w formie siatki oraz z siatki platynowej osadzonej na węglu. Otrzymane wyniki analizy spektroelektrochemicznej wykazały, że wraz ze wzrostem potencjału i piku prądowego absorpcja roztworu dsDNA wzrasta w sposób zależny od typu elektrody. Jednak położenie maksimum absorpcji nie zmieniało...
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A novel dual mode capacitor biosensor for real-time, label-free DNA detection
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Performance of a fuel cell optimized for in situ X-ray absorption experiments
PublicationKomercyjna komórka ogniwa paliwowego typu PEM została zmodyfikowana w celu realizacji pomiaru XAS w warunkach pracy ogniwa. Modyfikacja komórki miała na celu optymalizację jej parametrów pod kątem eksperymentu XAS realizowanego w modzie transmisyjnym i fluorescencyjnym, poprzez minimalizację absorpcji komórki, przy zachowaniu jej parametrów elektrochemicznych. W pracy zostały zaprezentowane szczegóły techniczne komórki a jej efektywność...