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International Journal of Evidence & Proof
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Iron–Sulfur Cluster Biogenesis Chaperones: Evidence for Emergence of Mutational Robustness of a Highly Specific Protein–Protein Interaction
PublicationBiogenesis of iron–sulfur clusters (FeS) is a highly conserved process involving Hsp70 and J-protein chaperones. However, Hsp70 specialization differs among species. In most eukaryotes, including Schizosaccharomyces pombe, FeS biogenesis involves interaction between the J-protein Jac1 and the multifunctional Hsp70 Ssc1. But, in Saccharomyces cerevisiae and closely related species, Jac1 interacts with the specialized Hsp70 Ssq1,...
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Validation of high-resolution 3D patient QA for proton PBS and IMPT using laser CT of improved polymer gel dosimeters
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Kinetic and equilibrium studies of the proton and deuteron transfer reaction between bis(2,4-dinitrophenyl)methane and strong nitrogen bases in acetonitrile
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SU‐E‐T‐103: Three‐Dimensional Measurements of Dose and LET from a Proton Beam via Polymer Gel Dosimetry
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Computational analysis of substituent effects on proton affinity and gas-phase basicity of TEMPO derivatives and their hydrogen bonding interactions with water molecules
PublicationThe study investigates the molecular structure of 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) and its derivatives in the gas phase using B3LYP and M06-2X functional methods. Intermolecular interactions are analyzed using natural bond orbital (NBO) and atoms in molecules (AIM) techniques. NO2-substituted TEMPO displays high reactivity, less stability, and softer properties. The study reveals that the stability of TEMPO derivatives...
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Pendant Proton-Relays Systematically Tune the Rate and Selectivity of Electrocatalytic Ammonia Generation in a Fe-Porphyrin Based Metal-Organic Framework
PublicationElectrocatalytic nitrite reduction (eNO2RR) is a promising alternative route to produce ammonia (NH3). Until now, several molecular catalysts have shown capability to homogeneously reduce nitrite to NH3, while taking advantage of added secondary-sphere functionalities to direct catalytic performance. Yet, realizing such control over heterogeneous electrocatalytic surfaces remains a challenge. Herein, we demonstrate that heterogenization...
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Electrochemical performance of Co3O4/CeO2 electrodes in H2S/H2O atmospheres in a proton-conducting ceramic symmetrical cell with BaZr0.7Ce0.2Y0.1O3 solid electrolyte
PublicationThe electrochemical performance of Co3O4/CeO2 mixed oxide materials as electrodes, when exposed to H2S/H2O atmospheres, was examined employing a proton conducting symmetrical cell, with BaZr0.7Ce0.2Y0.1O3 (BZCY72) as the solid electrolyte. The impact of temperature (700–850 °C) and H2S concentration (0–1 v/v%) in steam-rich atmospheres (90 v/v% H2O) on the overall cell performance was thoroughly assessed by means of electrochemical...
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Complementary Use of Multi-dimensional Gas Chromatography and Proton Transfer Reaction Mass Spectrometry for Identification of Rapeseed Oil Quality Indicators
PublicationThe change of concentration of various volatiles generated during frying may be an indicator of oil quality. Chemical compounds such as aldehydes, ketones, alcohols and carboxyl acids are the products of oxidation during thermal degradation of edible oils. Shown in this work is a complementary use of GC×GC-TOFMS and PTR-MS for the detection and determination of quality indicators of rapeseed oil. The former technique was used for...
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An integral-differential method for impedance determination of the hydrogen oxidation process in the presence of carbon monoxide in the proton exchange membrane fuel cell
PublicationThe impedance of a proton exchange membrane fuel cell powered by hydrogen contaminated with carbon monoxide, ranging from 150 to 300 ppb, is measured and discussed. The tested range of CO concentration complied with the fuel standard specified in the ISO standards. Studies of influence of CO contamination on operation of PEMFC are crucial for further development and commercialization of fuel cells for automotive applications. Based...
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Photon Physics and Plasma Research, Photonics Applications and Web Engineering, Wilga, May 2012
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Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island film
PublicationOpisano fluorescencję barwnika CY5 połączonego z DNA w roztworze buforowym na wysepkach srebrowych.
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The Prison System Review
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Some applications of thermophiles and their enzymes for protein processing
PublicationPrzedstawiono charakterystykę, właściwości oraz możliwości zastosowań enzymów proteolitycznych wytwarzanych przez termofile. Zaletą tych biokatalizatorów jest między innymi duża odporność na działanie rozpuszczalników organicznych, detergentów i innych czynników denaturujących białka oraz mała wrażliwość na zmiany pH. Dokonano też przeglądu czynników powodujących dużą stabilność proteaz z termofili.
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Pea Protein for Hempseed Oil Nanoemulsion Stabilization
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The Sense of Security of the Prison Service Offi cers
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The investigation of the effects of counterions in protein dynamics simulations
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Phonon Mechanism of the Magnetostructural Phase Transition in MnAs
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Phonon mode potential and its contribution to anharmonism
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Polysaccharide–Protein Complexes in a Coarse-Grained Model
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The Eliashberg study of the electron–phonon superconductivity inYSn3compound
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Phonon-mediated superconductivity in bismuthates by nonadiabatic pairing
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Axial piston pumps with camdriven commutation unit
PublicationPrzedstawiono wyniki badań prototypów pomp wielotłoczkowych osiowych wyposażonych w rozrząd krzywkowy oraz ich porównanie z rezultatami osiąganymi przez produkowane obecnie jednostki z rozrządem czołowym. Podano dane techniczne pomp nowego typu wchodzących do produkcji.
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Outline of marine piston engines' technology development.
PublicationW referacie przedstawiono najistotniejsze wydarzenia mające wpływ na produkcję i rozwój silników o zapłonie samoczynnym. Zasygnalizowano najistotniejsze osiągnięcia w rozwoju tych silników i podano nazwy firm, w których one zostały zastosowane. Podano także najważniejsze fakty związane z produkcją silników w Polsce.
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ANALYSIS OF THE p53 PROTEIN GENE EXPRESSION MODEL
PublicationWe study the asymptotic behaviour of the solutions of the p53-Mdm2 model proposed by Monk (2003). The p53 gene is crucial for cellular inhibition of the angiogenesis process, while Mdm2 is a negative regulator of the p53 tumor-suppressor. We investigate the stability of the positive steady state and perform some numerical experiments.
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Protein thermal stabilization in aqueous solutions of osmolytes
PublicationProteins’ thermal stabilization is a significant problem in various biomedical, biotechnological, and technological applications. We investigated thermal stability of hen egg white lysozyme in aqueous solutions of the following stabilizing osmolytes: Glycine (GLY), N-methylglycine (NMG), N,N-dimethylglycine (DMG), N,N,N-trimethylglycine (TMG), and trimethyl-N-oxide (TMAO). Results of CD-UV spectroscopic investigation were compared...
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Modelling gene expression of a self-regulating protein
PublicationWe analyze a model of gene transcription and protein synthesis. We take into account the number of sites on the protein’s promoter at which the protein’s dimers can bind blocking transcription of protein mRNA.
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Apple pectin complexes with whey protein isolate
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Taurine as a water structure breaker and protein stabilizer
PublicationThe enhancing effect on the water structure has been confirmed for most of the osmolytes exhibiting both stabilizing and destabilizing properties in regard to proteins. The presented work concerns osmolytes, which should be classified as “structure breaking” solutes: taurine and N,N,N-trimethyltaurine (TMT). Here, we combine FTIR spectroscopy, DSC calorimetry and DFT calculations to gain an insight into the interactions between...
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Problematyka implantacji protez piersiowych - przegląd literatury
PublicationOperacje związane z wykorzystaniem implantów piersi są jednymi z najczęściej przeprowadzanych zabiegów chirurgii plastycznej, dlatego istotne jest, aby zminimalizować ryzyko powikłań. Do najczęściej występujących zalicza się: obrzęk, wysięk, krwiaki, problemy z gojeniem ran oraz infekcje. Zakłada się, że infekcja jest głównym czynnikiem prowadzącym do degradacji implantu. Agresywne środowisko gospodarza, ciągłe zmiany pH i temperatury...
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The difficult heritage. The reuse of former prison buildings
PublicationIn recent years, there has been a trend to reuse abandoned buildings. Adaptive re-use allows preservation of the original structure and implements a new function to it. Such activity could help to preserve the historic value of buildings; moreover, many other advantages can be found in educational, ecological and economic fields. However, the question arises of whether every object can be freely adapted. This article examines the...
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Robustness of the Rotor-router Mechanism
PublicationW pracy rozważano model eksploracji grafu nieskierowanego przez pojedynczego agenta, w którym sterowanie agentem odbywa się zgodnie z zasadą ''rotor-router'' (inaczej: ''Propp machine''). Przeanalizowano czas stabilizacji agenta do trajektorii w postaci cyklu Eulera w przypadku wystąpienia zaburzeń w grafie: usunięcie krawędzi, dodanie krawędzi, lokalna zamiana portów
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HYBRID REDUCED MODEL OF ROTOR
PublicationIn the paper, the authors describe the method of model reduction of a rotor system. The proposed approach enables to obtain a low order model including e.g. nonproportional damping or the gyroscopic effect. This method is illustrated using the example of a rotor system. First, a model of the system was built without gyroscopic and damping effects by using the rigid finite element method. Next, this model was reduced. Finally, two...
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Structure and thermoelectric properties of Bi–Te alloys obtained by novel method of oxide substrates reduction
PublicationA novel method of thermoelectric materials fabrication has been developed. Oxide reagents have been melted at high temperature in air, quenched, milled to powder and reduced in hydrogen to form Bi–Te alloys. Structural and thermoelectric properties of samples have been investigated depending on initial composition and temperature of reduction process. Sample 25Bi2O3–75TeO2 reduced at 340 °C for 10 h has been found to be a p-type...
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Function and Frustration of Multi-Drug ABC Exporter Protein and Design of Model Proteins for Drug Delivery Using Protein Hydration Thermodynamics
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3D dose verification with polymer gel detectors of brain-spine match line for proton pencil beam cranio-spinal: A preliminary study
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Analysis of volatile fraction of sweetie (Citrus maxima × Citrus paradisi) and its parent fruit using proton transfer reaction mass spectrometry
PublicationThe quality of the fruit is affected by several main ingredients and the aroma plays a fundamental role during the selection of fruit by consumers. In the case where several fruit have similar aromas and only one of them has specific health properties, it is very important to find the differences in the volatile organic compounds (VOCs) composition to distinguish these samples. Such situations are often found for hybrid fruit....
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A new method for real-time monitoring of volatiles in frying fumes using proton transfer reaction mass spectrometry with time-of-flight analyse
PublicationTo safeguard the consumers’ well-being, it is necessary to develop novel methods for determination of carcinogens in food, including volatiles generated during frying. The currently used procedures for analysis of volatile fraction of vegetable oils are not based on real-time measurements and thus do not enable the determination of carcinogenic compounds in frying fumes; instead, only the headspace or liquid fraction is sampled....
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Investigation of the Dynamism of Nanosized SOA Particle Formation in Indoor Air by a Scanning Mobility Particle Sizer and Proton-Transfer-Reaction Mass Spectrometry
PublicationTerpenes are VOCs of particular importance, since they are emitted from a wide range of indoor sources and are considered to be precursors of Secondary Organic Aerosol (SOA) formation. It has been proven that SOA particles, especially nanosized ones, pose a threat to human health. In this research, experiments with the application of an environmental chamber and real-time measurement techniques were carried out to investigate in...
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Pupil detection supported by Haar feature based cascade classifier for two-photon vision examinations
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A DSC and NMR-Relaxation study of the molecular mobility of water protons interacting with chemically modified starches
PublicationChanges in the mobility of water protons in the chemically modified starches (CMS)–water system are studied by differential scanning calorimetry and NMR relaxation. The amounts of unfrozen water at negative temperatures and additional (after gelation) unfrozen for CMS are lower than those for native starch. The proton spin–spin relaxation time T2 for CMS samples, conventionally attributed to the water fraction in starch granules,...
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In vivo imaging of the human eye using a two-photon excited fluorescence scanning laser ophthalmoscope
PublicationBACKGROUND. Noninvasive assessment of metabolic processes that sustain regeneration of human retinal visual pigments (visual cycle) is essential to improve ophthalmic diagnostics and to accelerate development of new treatments to counter retinal diseases. Fluorescent vitamin A derivatives, which are the chemical intermediates of these processes, are highly sensitive to UV light; thus, safe analyses of these processes in humans...
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Pupil detection supported by Haar feature based cascade classifier for two-photon vision examinations
PublicationThe aim of this paper is to present a novel method, called Adaptive Edge Detection (AED), of extraction of precise pupil edge coordinates from eye image characterized by reflections of external illuminators and laser beams. The method is used for monitoring of pupil size and position during psychophysical tests of two-photon vision performed by dedicated optical set-up. Two-photon vision is a new phenomenon of perception of short-pulsed...
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Solid state versus fiber picosecond infrared lasers applied to two-photon vision tests
PublicationDevelopment of new microperimetric tools dedicated for imaging of early functional changes in the retina may help in the monitoring of various ocular diseases progression e.g. Age-Related Macular Degeneration. Recently described two-photon vision may be applied to microperimetric devices. Many subjects with well-known disease history could be investigated with newly developed instrumentation that tests ability of human eye to perceive...
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Tomasz Wąsowicz dr hab.
PeopleTomasz Wąsowicz's research was first related to high-resolution atomic spectroscopy and focused on measurements and analysis of the transition probabilities of the forbidden lines, the hyperfine and isotopic structure of spectral lines of heavy elements, Stark effect in the helium atom. Tomasz Wąsowicz currently studies physicochemical processes occurring during interactions of various forms of radiation with atoms and molecules...
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Characterization of a single-stranded DNA-binding-like Protein from Nanoarchaeum equitans - a nucleic acid binding protein with broad substrate specificity
PublicationBackground SSB (single-stranded DNA-binding) proteins play an essential role in all living cells and viruses, as they are involved in processes connected with ssDNA metabolism. There has recently been an increasing interest in SSBs, since they can be applied in molecular biology techniques and analytical methods. Nanoarchaeum equitans, the only known representative of Archaea phylum Nanoarchaeota, is a hyperthermophilic, nanosized,...
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Analyses of influence of the rotor slots' skew on inductances of the stator and rotor windings of a squirell-cage asynchronous motor
PublicationObwodowy model matematyczny silnika asynchronicznego klatkowego ujęty w osiach naturalnych zawiera indukcyjności zależne od kąta położenia wirnika. Indukcyjności te zawierają szereg wyższych harmonicznych zależnych od układu geometrycznego strefy przyszczelinowej. Jednym z elementów tego układu jest skos żłobków wirnika. W referacie przedstawiono wyniki obliczeń elektromagnetycznych silnika magnesowanego pojedynczą cewką uzwojenia...
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A bifunctional chimeric protein consisting of MutS and beta-galactoside
PublicationPraca pokazuje konstrukcję DNA plazmidowego kodującego bifunkcjonalne białko chimeryczne złożone z MutS Thermus thermophilus i beta-galaktozydazy E.coli, optymalizację jego ekspresji i oczyszczania. Białko to testowano w metodzie wykrywania mutacji punktowych, wykorzystując kolorymetryczne mierzenie aktywności domeny reporterowej beta galaktozydazy.
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Effect of increased protein intake on the risk of female infertility
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Platon Scientific HD TV Platform in PIONIER Network
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