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Wyniki wyszukiwania dla: DNA REPAIR ENZYMES
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Genotyping Techniques for Determining the Diversity of Microorganisms
PublikacjaTyping of microbial pathogens, or identifying bacteria at the strain level, is particularly important for diagnosis, treatment, and epidemiological surveillance of bacterial infections. This is especially the case for bacteria exhibiting high levels of antibiotic resistance or virulence, and those involved in nosocomial or pandemic infections. Strain typing also has applications in studying bacterial population dynamics. The part...
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Synthetic lipopeptides as potential topical therapeutics in wound and skin care: in vitro studies of permeation and skin cells behaviour
PublikacjaSeveral Gly-His-Lys analogues were obtained to investigate their antimicrobial properties. Three lipophilic analogues with the structure numbering 1b, 2b, 4b, exhibit significant effect against bacteria and were selected for in vitro evaluation of skin cells behaviour. In the present studies, an in vitro model of wound repair, proliferative cell staining, and tracking of living cells were used. Cell proliferation and cell migration,...
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A simple modification to improve the accuracy of methylation-sensitive restriction enzyme quantitative polymerase chain reaction
PublikacjaDNA digestion with endonucleases sensitive to CpG methylation such as HpaII followed by polymerase chain reaction (PCR) quantitation is commonly used in molecular studies as a simple and inexpensive solution for assessment of region-specific DNA methylation. We observed that the results of such analyses were highly overestimated if mock-digested samples were applied as the reference.We determined DNA methylation levels in several...
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Virtual Engineering Objects: Effective Way of Knowledge Representation and Decision Making
PublikacjaThis paper presents a knowledge representation case study by constructing Decisional DNA of engineering objects. Decisional DNA, as a knowledge representation structure not only offers great possibilities on gathering explicit knowledge of formal decision events but also it is a powerful tool for decision-making process. The concept of Virtual engineering Object (VEO), which is a knowledge and experience representation of engineering...
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VOLTAMMETRIC DETERMINATION OF DEOXYRIBONUCLEIC ACID PRESENCE ON A CARBON ELECTRODE
PublikacjaThis paper presents a voltammetry as a useful method to analyse DNA from different species. This electrochemical method is well know and used in different analyses. The influence of an area of working electrode and the capacity component, is shown at equals and the measurement. In voltammetry DNA measurement the capacity component is very important and must be taken into consideration. All mathematical analyses were verify at laboratory.
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Wiązanie się do DNA pochodnych 4-metylo-1-nitroakrydyny wykazujących właś-ciwości przeciwnowotworowe.**2003, 116 s. 23 rys. 12 tab. bibliogr. 163 poz. maszyn. Rozprawa doktorska /09.05.2003/ P. Gdań. Wydz. Chem. Promotor: prof. dr hab. inż. J. Konopa.
Publikacja.
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Novel primosomal protein B from Clostridium pastuerianum
PublikacjaPriB is a primosomal protein that catalyzes DNA replication in Procaryota. The replication pathway starts with PriA protein - the initiator protein that binds to a DNA replication fork, unwinds double-stranded DNA and role of PriB is to stabilize PriA on the DNA. However there are many biochemical differences in replication mechanism in bacteria and only some of them use PriB proteins. A few of PriB proteins were published and...
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Novel primosomal protein B from Clostridium pasteurianum
PublikacjaPriB is a primosomal protein that catalyzes DNA replication in Procaryota. The replication pathway starts with PriA protein - the initiator protein that binds to a DNA replication fork, unwinds double-stranded DNA and role of PriB is to stabilize PriA on the DNA. However there are many biochemical differences in replication mechanism in bacteria and only some of them use PriB proteins. A few of PriB proteins were published and...
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Multisine impedimetric probing of biocatalytic reactions for label-free detection of DEFB1 gene: How to verify that your dog is not human?
PublikacjaAlbert is a dog (Canis familiaris), but he does not realize this. Albert loves human food (and beer), watching movies on the internet, sleeping in bed, and more. But he should not do all these things. To convince him that, we have desinged a test procedure. The DEFB1 gene is unique to human species. Detecting its presence from saliva and in short periods may offer an advantage in the field of forensic medicine, and influence Albert’s...
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A Strategy for Managing the Operation of Technical Infrastructure Based on the Analysis of “Bad Actors”—A Case Study of LOTOS Group S.A.
PublikacjaThe article covers issues related to the selection of a management strategy for the operation of technical infrastructure. It contains the approaches, methodology and a general model of the system of operation of the Maintenance Services of the LOTOS Group production company, which is an example of a large oil concern. Nowadays, an increasing aspect is put on the efficiency of individual repair processes, as well as the operation...
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Modulation of carcinogen metabolizing cytochromes p450 in rat liver and kidney by cabbage and sauerkraut juices: comparison with the effects of indole-3-carbinol and phenethyl isothiocyanate
PublikacjaThis study investigated the effect of raw cabbage and sauerkraut juices on the activity and expression of CYP1A1, 1A2, 1B1 and 2B in Wistar rat liver and kidney. The results were compared with those of two commercially available products of glucosinolates degradation: indole-3-carbinol (I3C) and phenethyl isothiocyanate (PEITC). Significant differences in the effect of the cabbage juices as well as I3C and PEITC between the liver...
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Real-Time PCR: molecular technique of many applications
PublikacjaReal-Time PCR is a sensitive DNA amplification technique initially applied in genetics and molecular biology. It enables in vivo copying of the selected DNA fragment (flanked by two primers) by the thermostable polymerase (in the presence of magnesium ions and deoxynucleotide triphosphates) and simultaneous measurement of the fluorescence. For one or more specific sequences in a DNA sample, real-time PCR enables both detection...
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Spectroelectroanalytical properties of antitumor agent C1311
PublikacjaZastosowano kilka metod woltamperometrycznych dla charakterystyki właściwości elektrochemicznych przeciwnowotworowej pochodnej imidazoakrydonu o symbolu C-1311. Wyniki wskazały, że możliwym będzie zastosowanie metod elektrochemicznych do badania oddziaływań badanego związku z DNA. Badania spektroelektrochemiczne ujawniły, że oscylacyjne zmiany absorbcji związku obserwowane po cyklicznej polaryzacji elektrody. Oscylacje te znikały...
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ALIS-FLP: amplified ligation selected fragment-length polymorphism method for microbial genotyping
PublikacjaA DNA fingerprinting method known as ALIS-FLP (amplified ligation selected fragment-length polymorphism) has been developed for selective and specific amplification of restriction fragments from TspRI restriction endonuclease digested genomic DNA. The method is similar to AFLP, but differs in that only one specific restriction enzyme (TspRI) is used. The cohesive ends of the DNA fragments are ligated with two types of oligonucleotide....
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Instalacja i wstępna analiza danych batymetrycznych pochodzących z echosondy wielowiązkowej
PublikacjaW akustycznym telemonitoringu środowiska morskiego stosowane są dwa rodzaje sonarów, a mianowicie: sonary boczne i echosondy wielowiązkowe. Oba systemy dostarczają danych zarówno dla batymetrii, jak i dla akustycznego obrazowania dna morskiego. Echosondy wielowiązkowe są zaawansowanymi technologicznie urządzeniami, które dzięki wielokierunkowej obserwacji szerokokątnej dna, przy jednoczesnym utrzymaniu wysokiej rozdzielczości kątowej,...
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STUDY ON THE RELATIONSHIP BETWEEN VEHICLE MAINTENANCE AND FUEL CONSUMPTION
PublikacjaA contemporary road vehicle (RV) is a rather complex system, consisting of a large number of subsystems, assemblies, units, and elements (parts). While operating, an RV interacts with the environment, and its elements interact with each other. Consequently, the properties (parameters) of these elements change in the process - hardness, roughness, size, relative position, gapping, etc. A partial solution to the presented problems...
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Discrimination of hospital isolates of Acinetobacter baumannii using repeated sequences and whole genome alignment differential analysis
PublikacjaAn optimized method for bacterial strain differentiation, based on combination of Repeated Sequences and Whole Genome Alignment Differential Analysis (RS&WGADA), is presented in this report. In this analysis, 51 Acinetobacter baumannii multidrug-resistance strains from one hospital environment and patients from 14 hospital wards were classified on the basis of polymorphisms of repeated sequences located in CRISPR region, variation...
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Adaptive neuro fuzzy and fuzzy decision tree classifiers as applied to sea floor characterization.
PublikacjaPrzedstawiono wyniki badań wpływu różnych parametrów echa odbitego od dna morskiego na dokładność klasyfikacji typu dna przy pomocy sieci neuronowej z logiką rozmytą i przy pomocy drzew decyzyjnych. W szczególności uwzględniono takie parametry echa jak: energia, amplituda i nachylenie opadającego zbocza, wzbogacone o współczynniki falkowe otrzymane z dyskretnej transformacji falkowej (DWT).
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Two-state dynamics for replicating two-strand systems
PublikacjaDynamika dwustanowa została zastosowana do opisów układów dwuniciowych, analogicznych do DNA.
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Modulation of CYP1A1, CYP1A2 and CYP1B1 expression by cabbage juices and indoles in human breast cell lines
PublikacjaEpidemiological studies have shown that consumption of cab- bage and sauerkraut is connected with significant reduction of breast cancer incidences. Estrogens are considered a major breast cancer risk factor and their metabolism by P450 enzymes substan- tially contributes to carcinogenic activity. The aim of this study was to investigate the effect of cabbage and sauerkraut juices of differ- ent origin on the expression profile...
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Nanostructured biocatalysis for biotechnological applications
PublikacjaThe purpose immobilization process is to enhance the performance of an enzyme for commercial processes. A large number of structures have been reported in the literature to boost the effectiveness of immobilized enzymes. The nanomaterials have the optimal properties for equilibrating key parameters that govern the performance of biocatalysts, such as high enzyme loading ability, specific surface area, and mass transfer resistance....
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Homocitrate synthase gene as a new molecular target in diagnostics of Candida spp. infections
PublikacjaNew potential molecular target which will be investigated during this research (homocitrate synthase gen) is the innovative target other than ribosomal DNA. This gene encodes enzyme present only in fungi and some Archaea what significantly increases specificity. It also reduces risk of contamination or influence of other factors (reaction with other DNA present in sample derived from bacteria or host cells) what excludes false...
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System do diagnostyki dermatofitowych zakażeń powierzchniowych oparty na technice Real-Time PCR
PublikacjaDermatofity należą do blisko spokrewnionej grupy grzybów, które wykazują wysokie powinowactwo do skeratynizowanych tkanek. Cecha ta czyni je odpowiedzialnymi za powierzchniowe grzybice skóry, paznokci oraz włosów. Szacuje się, iż nawet do 20% ludzi na całym świecie dotkniętych jest infekcjami powodowanymi przez dermatofity. Ich leczenie wymaga długotrwałego zastosowania leków przeciwgrzybiczych. W celu dobrania odpowiedniego leczenia...
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The concept of estimation of elevator shaft control measurement results in the local 3D coordinate system.
PublikacjaGeodetic control measurements play an important part because they provide information about the current state of repair of the construction, which has a direct impact on the safety assessment of its exploitation. Authors in this paper have focused on control measurements of the elevator shaft. The article discusses the problem of determining the deviation of elevator shaft walls from the vertical plane in the local 3D coordinate...
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Evaluation and cellular responses of modulators of TRF1/TRF2 protein’s function as potential anticancer drugs interfering with telomeric shelterin’s function
PublikacjaA number of proteins that interact with telomeres have been identified in human cells, indicating the high plasticity of human nucleoprotein complex organization. The most important complex is the "shelterin" complex, which consists of six proteins: TRF1, TRF2, TIN2, POT1, TPP1. The TRF1 and TRF2 directly bind to telomeric double-stranded DNA and the TIN2 protein. The TIN2 protein also binds to the TPP1 protein, stabilizing the...
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Selected results of the parametric sounding of the Gdansk Bay
PublikacjaW pracy przedstawiono metodykę i wyniki sondowania dna Zatoki Gdańskiej echosondą parametryczną.
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Experience Based Clinical Decision Support Systems: An Overview and Case Studies
PublikacjaThis chapter briefly overviews the evolution of the application of the Decisional DNA and the Set of Experience Knowledge Structure (SOEKS) in the medical domain and in particular in the specific case of the experience-based decision support systems. Decisional DNA, as a knowledge representation structure, offers great possibilities on gathering explicit knowledge of formal decision events as well as a tool for decision making...
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Immunological response of sheep to injections of plasmids encoding Toxoplasma gondii SAG1 and ROP1 genes
PublikacjaInfection with the intracellular protozoan parasite Toxo¬plasma gondii (T. gondii) causes health problems to both humans and livestock and has a large economic impact worldwide. The immune response in sheep following infection with T. gondii was evaluated using six different combinations of plasmid DNA, recombinant antigen and adjuvant. Sheep were generally vaccinated twice by intramuscular injection with plasmid DNA containing...
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The Toolbox of Methods for Multidirectional Characterization of Dietary Nucleic Acids; Verification for Raw and Processed Food Products
PublikacjaCurrently, the nutritional value of food is associated mainly with components such as proteins, carbohydrates, and lipids. However, another important macromolecules present in many foods are dietary nucleic acids (dietNA), i.e., DNA as well as both coding and non-coding RNAs. In the context of food chemistry and nutrition, dietNA are nowadays vastly neglected. In consequence, there are no dedicated methodologies to characterize...
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Underwater Welding of Duplex Stainless Steel
PublikacjaThe present work was conducted to assess the weldability of duplex stainless steel at underwater conditions. Interest of underwater welding of this steel grade is connected with necessity of preparing welding repair technologies for subsea pipelines widely used in offshore oil and gas industry. The GMA local cavity welding method was used in the investigations. Welded beads were performed underwater at 0.5 m depth and in the air....
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Dynamic Response of the Suspended on a Single Cable Footbridge
PublikacjaThe article presents numerical simulations, dynamic in situ load tests and a structural health monitoring (SHM) system installed in a suspended on a single cable footbridge. Numerical simulations performed prior to construction indicated the possibility of structural dynamics problems, finally confirmed in the course of dynamic test loading. In the dynamic load course the bridge deck developed vibrations displaying accelerations...
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Regression points in non-intrusive polynomial chaos expansion method and D-optimal design
PublikacjaThe paper addresses selected issues of uncertainty quantification in the modelling of a system containing surgical mesh used in ventral hernia repair. Uncertainties in the models occur e.g. due to variability of abdominal wall properties among others. In order to include them, a non-intrusive regression-based polynomial chaos expansion method is employed. Its accuracy depends on the choice of regression points. In the study a relation...
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Developing an Ontology from Set of Experience KnowledgeStructure
PublikacjaWhen referring to knowledge forms,collecting for all decision eventsin a knowledge-explicit way becomes a significant ask for any company. Set of experience knowledge structure can assis in accomplishing this purpose.However,after collecting,distributing and sharing that knowledge as adecisional DNA is even a more important advance.Distributing and sharing companies' decisional DNA through an efficient development of Ontologies...
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Biomechanical causes for failure of the Physiomesh/Securestrap system
PublikacjaThis study investigates the mechanical behavior of the Physiomesh/Securestrap system, a hernia repair system used for IPOM procedures associated with high failure rates. The study involved conducting mechanical experiments and numerical simulations to investigate the mechanical behavior of the Physiomesh/Securestrap system under pressure load. Uniaxial tension tests were conducted to determine the elasticity modulus of the Physiomesh...
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Electrically Conductive Carbon‐based (Bio)‐nanomaterials for Cardiac Tissue Engineering
PublikacjaA proper self-regenerating capability is lacking in human cardiac tissue which along with the alarming rate of deaths associated with cardiovascular disorders makes tissue engineering critical. Novel approaches are now being investigated in order to speedily overcome the challenges in this path. Tissue engineering has been revolutionized by the advent of nanomaterials, and later by the application of carbon-based nanomaterials...
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Badania zespołów przekazujących małe ryby z urządzenia pozycjonującego do zespołu obróbczego
PublikacjaW pracy przedstawiono badania oraz ich wyniki, przekazywania szprota w kanałach o oryginalnej schodkowej konstrukcji dna.
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Genotoxic properties of glucosinolates and isothiocyanates present in Brassica vegetables
PublikacjaThe bioactive phytochemicals found in Brassica vegetables, in this number glucosinolates (GLS), as well as products of their degradation such as isothiocyanates (ITC) and indoles, are regarded as the most promising chemopreventive compounds. These secondary metabolites display stron antibiological activity and constitute defence system preventing the development of agrophages attacking Brassica plants. Such properties suggest...
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Experience based knowledge representation for Internet of Things and Cyber Physical Systems with case studies
PublikacjaCyber Physical Systems and Internet of Things have grown significant attention from industry and academia during the past decade. The main reason behind this interest is the capabilities of such technologies to revolutionize human life since they appear as seamlessly integrating classical networks, networked objects and people to create more efficient environments. However, enhancing these technologies with intelligent skills becomes...
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Novel application of terminal restriction fragments length polymorphism method (t-RFLP) for identification of six clinically important Candida sp.
PublikacjaTerminal restriction fragments length polymorphism method (t-RFLP) is a modified classical RFLP method. The main difference is based on carrying out PCR using a fluorescently labeled primer or primers. The result of such modification is that after digestion of amplicons only terminal fragments of PCR product (containing fluorescent dye) are visualized. T-RFLP method has found application in environmental microbiology and epidemiology....
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Expression of a GDSL esterase from Pseudomonas sp. S9 in Pichiapastoris
PublikacjaCold active lipolytic enzymes are promising to replace the conventional enzymes processes of biotechnological industries. One of the most important feature of the cold-active lipases and esterases is that they offer economic benefits through energy saving. In general, they exhibit high activity at low temperatures and low thermostability at moderate temperatures. Lipolytic enzyme EstS9 was isolated from Pseudomonas sp. S9. A multiple...
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Organic solvents aggregating and shaping structural folding of protein, a case study of the protease enzyme
PublikacjaLow solubility of reactants or products in aqueous solutions can result in the enzymatic catalytic reactions that can occur in non-aqueous solutions. In current study we investigated aqueous solutions containing different organic solvents / deep eutectic solvents (DESs) that can influence the protease enzyme's activity, structural, and thermal stabilities. Retroviral aspartic protease enzyme is responsible for the cleavage of the...
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Nanobiocatalysis for biofuel production
PublikacjaThe human race is relied on the use of conventional and non-renewable energy resources like fossil fuels for centuries. With the rising environmental issues linked with the use of these conventional energy resources, the trend soon shifted towards more robust and sustainable energy resources. In this regard, the different generations of biofuels have revolutionized the industrial sector by ensuring an eco-friendly and more economical...
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Proteases immobilized on nanomaterials for biocatalytic, environmental and biomedical applications: Advantages and drawbacks
PublikacjaProteases have gained significant scientific and industrial interest due to their unique biocatalytic characteristics and broad-spectrum applications in different industries. The development of robust nanobiocatalytic systems by attaching proteases onto various nanostructured materials as fascinating and novel nanocarriers has demonstrated exceptional biocatalytic performance, substantial stability, and ease of recyclability over...
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On long-term prediction of stresses in principal members of ship hull structures.
PublikacjaOpisano krytycznie wymagania towarzystw klasyfikacyjnych dotyczące oceny wytrzymałości wzdłużnych wiązarów i wzdłużnych usztywnień poszycia kadłuba statku. Przedstawiono sposób prognozowania długoterminowego dynamicznych składowych tych naprężeń oraz zaproponowano sposób składania charakterystycznych wartości poszczególnych składowych naprężeń do zastosowania w przepisach Polskiego Rejestru Statków. Sposób ten bazuje na koncepcji...
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KEMR-Net: A Knowledge-Enhanced Mask Refinement Network for Chromosome Instance Segmentation
PublikacjaThis article proposes a mask refinement method for chromosome instance segmentation. The proposed method exploits the knowledge representation capability of Neural Knowledge DNA (NK-DNA) to capture the semantics of the chromosome’s shape, texture, and key points, and then it uses the captured knowledge to improve the accuracy and smoothness of the masks. We validate the method’s effectiveness on our latest high-resolution chromosome...
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ANALYSIS OF THE INCREASE OF CONSTRUCTION COSTS IN URBAN REGENERATION PROJECTS
PublikacjaThe subject of this paper is the analysis and evaluation of the reasons for construction costs increases in urban regeneration projects. The analysis considers major refurbishments of real estates’, as well as heavy repair and modernization of the road system. For the period mentioned, the costs from works and expenditure schedules were compared with the costs of additional works, which shows the percentage cost increase in relation...
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Integrating Experience-Based Knowledge Representation and Machine Learning for Efficient Virtual Engineering Object Performance
PublikacjaMachine learning and Artificial Intelligence have grown significant attention from industry and academia during the past decade. The key reason behind interest is such technologies capabilities to revolutionize human life since they seamlessly integrate classical networks, networked objects and people to create more efficient environments. In this paper, the Knowledge Representation technique of Set of Experience...
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Making digital TV smarter: capturing and resusing experience in digital TV
PublikacjaZaproponowano inteligentny system wspomagania funkcjonowania telewizji cyfrowej opraty na decyzyjnym DNA oraz formalizacji wiedzy w postaci zbioru doswiadczen.
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On the time behaviour of Okazaki fragments
PublikacjaW pracy wyprowadzono wzory na prawdopodobieństwo, że podczas procesu replikacji DNA w chwili t pojawi się n fragmentów Okazaki.
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Rational design, synthesis and biological evaluation of thiadiazinoacridines: a new class of antitumor agents.
PublikacjaZsyntetyzowano serię związków o potencjalnej zdolności do wiązania się z DNA z grupy tiadiazinoakrydyn poprzez cyklizację odpowiednich pochodnych 9-imino-4-nitro-9,10-dihydroakrydyny. Zbadano ich zdolność do wiązania się z DNA oraz cytotoksyczność względem kilku linii komórek nowotworwych. Badania aktywności przeciwnowotworowej wobec białaczki P388 wskazały na zgodność tych wyników z wynikami cytotoksyczności in vitro. Wykazano,...