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CaZnO-based nanoghosts for the detection of ssDNA, pCRISPR and recombinant SARS-CoV-2 spike antigen and targeted delivery of doxorubicin
PublikacjaOverexpression of proteins/antigens and other gene-related sequences in the bodies could lead to significant mutations and refractory diseases. Detection and identification of assorted trace concentrations of such proteins/antigens and/or gene-related sequences remain challenging, affecting different pathogens and making viruses stronger. Correspondingly, coronavirus (SARS-CoV-2) mutations/alterations and spread could lead to overexpression...
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The New Test Procedure for Group-Type Composition of Base Oils of Lubricating Oils, Especially Emitted into the Environment
PublikacjaMineral base oil, a product of multistep fine refining of the relevant fractions from vacuum distillation of crude oil, is a main component of so-called mineral lubricating oils containing aliphatic and alicyclic substituted aromatic hydrocarbons, i.e., derivatives of benzene and biphenyl. Mineral lubricating oil is composed mostly of mineral base oil and a low amount of enriching additives, most often products of advanced organic...
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Recent advancements in LC‐MS based analysis of biotoxins: Present and future challenges
PublikacjaThere has been a rising concern regarding the harmful impact of biotoxins, source of origin, and the determination of the specific type of toxin. With numerous reports on their extensive spread, biotoxins pose a critical challenge to figure out their parent groups, metabolites, and concentration. In that aspect, liquid chromatography‐mass spectrometry (LC‐MS) based analysis paves the way for its accurate identification and quantification....
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Establishing the allosteric mechanism in CRISPR‐Cas9
PublikacjaAllostery is a fundamental property of proteins, which regulates biochemical information transfer between spatially distant sites. Here, we report on the critical role of molecular dynamics (MD) simulations in discovering the mechanism of allosteric communication within CRISPR‐Cas9, a leading genome editing machinery with enormous promises for medicine and biotechnology. MD revealed how allostery intervenes during at least three...
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A Qualitative Study on Non-functional Requirements in Agile Software Development
PublikacjaWidespread use of agile software development (ASD) methods can be observed nowadays. Non-functional requirements (NFRs) are often reported to be a problematic issue for agile projects, since ASD methods tend to neglect NFRs while focusing on incremental delivery of functional features. Despite that, only very few studies have explored the requirements engineering practices used in ASD and dedicated particularly to NFRs. Objective:...
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On Fast Multi-objective Optimization of Antenna Structures Using Pareto Front Triangulation and Inverse Surrogates
PublikacjaDesign of contemporary antenna systems is a challenging endeavor, where conceptual developments and initial parametric studies, interleaved with topology evolution, are followed by a meticulous adjustment of the structure dimensions. The latter is necessary to boost the antenna performance as much as possible, and often requires handling several and often conflicting objectives, pertinent to both electrical and field properties...
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Discrimination of hospital isolates of Acinetobacter baumannii using repeated sequences and whole genome alignment differential analysis
PublikacjaAn optimized method for bacterial strain differentiation, based on combination of Repeated Sequences and Whole Genome Alignment Differential Analysis (RS&WGADA), is presented in this report. In this analysis, 51 Acinetobacter baumannii multidrug-resistance strains from one hospital environment and patients from 14 hospital wards were classified on the basis of polymorphisms of repeated sequences located in CRISPR region, variation...
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Tailoring properties of indium tin oxide thin films for their work in both electrochemical and optical label-free sensing systems
PublikacjaThis work is devoted to the identification properties of indium tin oxide (ITO) thin films responsible for their possible application in combined optical and electrochemical label-free sensing systems offering enhanced functionalities. Since any post-processing would make it difficult to identify direct relation between deposition parameters and properties of the ITO films, especially when deposition on temperature-sensitive substrates...
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Expedited Acquisition of Database Designs for Reduced-Cost Performance-Driven Modeling and Rapid Dimension Scaling of Antenna Structures
PublikacjaFast replacement models have been playing an increasing role in high-frequency electronics, including the design of antenna structures. Their role is to improve computational efficiency of the procedures that normally entail large numbers of expensive full-wave electromagnetic (EM) simulations, e.g., parametric optimization or uncertainty quantification. Recently introduced performance-driven modeling methods, such as the nested...
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Constrained multi-objective optimization of compact microwave circuits by design triangulation and pareto front interpolation
PublikacjaDevelopment of microwave components is an inherently multi-objective task. This is especially pertinent to the design closure stage, i.e., final adjustment of geometry and/or material parameters carried out to improve the electrical performance of the system. The design goals are often conflicting so that the improvement of one normally leads to a degradation of others. Compact microwave passives constitute a representative case:...
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Bee Pollen and Bee Bread as a Source of Bacteria Producing Antimicrobials
PublikacjaThe principal objective of the study was the isolation and identification of bacteria that are present in mature bee bread (BB) and dried (ready for selling and consumption) bee pollen (BP). Obtained isolates were screened for their potential to inhibit select human pathogenic bacteria and their ability to produce enzymes of particular industrial importance. Four and five samples of BP and BB, respectively, were used for the study....
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Global Design Optimization of Microwave Circuits Using Response Feature Inverse Surrogates
PublikacjaModern microwave design has become heavily reliant on full-wave electromagnetic (EM) simulation tools, which are necessary for accurate evaluation of microwave components. Consequently, it is also indispensable for their development, especially the adjustment of geometry parameters, oriented towards performance improvement. However, EM-driven optimization procedures incur considerable computational expenses, which may become impractical...
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Antifungal Effect of Penicillamine Due to the Selective Targeting of L-Homoserine O-Acetyltransferase
PublikacjaDue to the apparent similarity of fungal and mammalian metabolic pathways, the number of established antifungal targets is low, and the identification of novel ones is highly desirable. The results of our studies, presented in this work, indicate that the fungal biosynthetic pathway of L-methionine, an amino acid essential for humans, seems to be an attractive perspective. The MET2 gene from Candida albicans encoding L-homoserine...
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Sources and composition of chemical pollution in Maritime Antarctica (King George Island), part 1: Sediment and water analysis for PAH sources evaluation in the vicinity of Arctowski station
PublikacjaThe paper presents a study regarding the identification of polycyclic aromatic hydrocarbons (PAHs) in fresh waters and surface sediments on the western shore of Admiralty Bay over four sampling seasons from 2017 to 2018. The results were compared to literature data from 2016 to provide a more comprehensive image of the environmental fate of PAHs over the years. The highest value of Σ PAHs was 82.9 ng/L and 445 ng/g dw in water...
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Comparative analysis of mRNA transcripts of HT-29 cell line expressed in identical quantities for pathogenic E. coli strains UM146 and UM147 with control Escherichia coli Nissle 1917
PublikacjaAim of study was comparative analysis of mRNA transcripts of HT-29 cell line, expressed in identical quantities for the combination of pathogenic and non-pathogenic Escherichia coli strains. HT-29 confluent monolayers infection with two pathogenic E. coli strains UM146 and UM147 resulted in two sets of mRNA transcripts that were identical with RNA transcripts obtained for non-pathogenic one strain E. coli Nissle 1917. In this study...
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Modeling and control of a redundantly actuated variable mass 3RRR planar manipulator controlled by a model-based feedforward and a model-based-proportional-derivative feedforward–feedback controller
PublikacjaIn the paper, dynamics of a complex mechatronics system is considered. A redundantly actuated planar manipulator is the base of the mechanical part of it. It is a 3RRR 1 platform based parallel manipulator. To control its trajectory, a model-based feedforward controller is employed. Three aspects are fundamental in the presented investigations. The first focus is on development of an accurate numerical model used to solve the inverse...
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Sub-regional service centres in reality and regional planning in Poland = Podregionalne osrodki obsługi w sferze realnej i planowaniu regionalnym w Polsce
PublikacjaSub-regional centres are defined as towns where the services of the sub-regional level are concentrated. In Poland these are such services that can be located in several towns in a voivodeship (region), but not in every capital of a poviat (county). The transformation of civilization has influenced changes in Christaller’s model of settlement network, but re-gularities are still valid. Models derived from Christaller's Central...
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Linear game non-contextuality and Bell inequalities—a graph-theoretic approach
PublikacjaWe study the classical and quantum values of a class of one-and two-party unique games, that generalizes the well-known XOR games to the case of non-binary outcomes. In the bipartite case the generalized XOR(XOR-d) games we study are a subclass of the well-known linear games. We introduce a 'constraint graph' associated to such a game, with the constraints defining the game represented by an edge-coloring of the graph. We use the...
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Level of public finances decentralization in European Union countries
PublikacjaResearch background: Despite of the universality of the implementation in democratic countries the principle of decentralization resulting from the belief that it is an instrument to improve the efficiency of public funds management, both the scope of public services and the level of decentralization in individual countries are not identical. Purpose of the article: Comparison the scope of fiscal decentralization...
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Refinement of the Hardening Soil model within the small strain range
PublikacjaThe popularity of the elasto-plastic Hardening Soil (HS) model is based on simple parameter identification from standard testing and empirical formulas. The HS model is implemented in many commercial FE codes designed to analyse geotechnical problems. In its basic version, the stress–strain behaviour within the elastic range is subject to the hypoelastic power law, which assures the barotropy of the elastic stiffness. However,...
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Detection of Bioactive Compounds in Organically and Conventionally Grown Asparagus Spears
PublikacjaIn the recent reports, there are contradictory conclusions about the nutritional and health properties of organic and conventionally growing vegetables. We hypothesized that organic cultivation system results in higher quality of asparagus (Asparagus officinalis L.) because of organic manure and effective organisms. Therefore, new analytical methods were applied in order to find the differences in bioactive compounds between the...
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Complementary use of GCxGC–TOF–MS and statistics for differentiation of variety in biosolid samples
PublikacjaFormation of biosolid cakes, which are one of the main wastes generated in wastewater treatment plants, is connected with emission of many hazardous chemical compounds, including odoriferous ones. To optimize particular processes of biosolid cake processing, it is necessary to assess chemical composition of the gas mixtures containing the compounds released from the cakes. The paper proposes application of two-dimensional gas chromatography...
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Machining process sequencing and machine assignment in generative feature-based CAPP for mill-turn parts
PublikacjaProcess selection and sequencing, as one of the most complex issues when evaluated from a mathematical point of view and crucial in CAPP, still attract research attention. For the current trend of intelligent manufacturing, machining features (MFs) are the information carriers for workpiece geometry and topology representation. They are basically derived from CAD models and are used by downstream engineering applications. A feature-based...
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Singlet oxygen in the removal of organic pollutants: An updated review on the degradation pathways based on mass spectrometry and DFT calculations
PublikacjaThe degradation of pollutants by a non-radical pathway involving singlet oxygen (1O2) is highly relevant in advanced oxidation processes. Photosensitizers, modified photocatalysts, and activated persulfates can generate highly selective 1O2 in the medium. The selective reaction of 1O2 with organic pollutants results in the evolution of different intermediate products. While these products can be identified using mass spectrometry...
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Exploring the landscape of automatic cerebral microbleed detection: A comprehensive review of algorithms, current trends, and future challenges
PublikacjaThis paper provides the first review to date which gathers, describes, and assesses, to the best of our knowledge, all available publications on automating cerebral microbleed (CMB) detection. It provides insights into the current state of the art and highlights the challenges and opportunities in this topic. By incorporating the best practices identified in this review, we established guidelines for the development of CMB detection...
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Current advances in membrane processing of wines: A comprehensive review
PublikacjaBackground Membrane-based operations, especially pressure-driven membrane operations, are today well-established procedures for various applications in the wine industry thanks to their intrinsic properties and undoubted advantages over traditional methods. Emerging membrane processes, such as pervaporation, electrodialysis and osmotic distillation, forward osmosis, membrane contactors, offer new and interesting perspectives to...
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Multi-Analytical Techniques for the Study of Burial Clothes of Polish King Sigismund III Vasa (1566–1633) and His Wife Constance Habsburg (1588–1631)
PublikacjaThe subjects of this research are the burial clothes of Polish King Sigismund III Vasa and his wife Constance, which were woven and embroidered with silk and metal threads. Fragments of the textiles underwent spectroscopic, spectrometric, and thermogravimetric analyses. The hydrofluoric acid extraction method was improved to isolate various classes of dyes from the textile samples that had direct contact with human remains. High-performance...
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Conditions for increasing the recognition of degradation in thermal-flow diagnostics, taking into account environmental legal aspects
PublikacjaThe ever-increasing demand for electricity and the need for conventional sources to cooperate with renewable ones generates the need to increase the efficiency and safety of the generation sources. Therefore, it is necessary to find a way to operate existing facilities more efficiently with full detection of emerging faults. These are the requirements of Polish, European and International law, which demands that energy facilities...
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Broadband/Dual-band Metal-Mountable UHF RFID Tag Antennas: A Systematic Review. Taxonomy Analysis, Standards of Seamless RFID System Operation, Supporting IoT implementations, Recommendations and Future Directions
PublikacjaThe employment of broadband/dual-band ultra-high frequency (UHF) radio frequency identification (RFID) tag antennas contributes to the growth of RFID technology, with many potential implications, such as the increase of international trade, and reducing costs thereof. This study presents all reported articles on RFID tags for metal objects that can work seamlessly across different countries. Moreover, it addresses all available...
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On Unsupervised Artificial-Intelligence-Assisted Design of Antennas for High-Performance Planar Devices
PublikacjaDesign of modern antenna structures is a challenging endeavor. It is laborious, and heavily reliant on engineering insight and experience, especially at the initial stages oriented towards the devel-opment of a suitable antenna architecture. Due to its interactive nature and hands-on procedures (mainly parametric studies) for validating suitability of particular geometric setups, typical antenna development requires many weeks...
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The effects of the IM1-12Br ionic liquid and the oxytetracycline mixture on selected marine and brackish microorganisms
PublikacjaThe number of applications and commercialized processes utilizing ionic liquids has been increasing, and it is anticipated that this trend will persist and even intensify in the future. Ionic liquids possess desirable characteristics, such as low vapor pressure, good water solubility, amphiphilicity, and stability. Nevertheless, these properties can influence their environmental behavior, resulting in resistance to biotic and abiotic...
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Discriminating macromolecular interactions based on an impedimetric fingerprint supported by multivariate data analysis for rapid and label-free Escherichia coli recognition in human urine
PublikacjaThis manuscript presents a novel approach to address the challenges of electrode fouling and highly complex electrode nanoarchitecture, which are primary concerns for biosensors operating in real environments. The proposed approach utilizes multiparametric impedance discriminant analysis (MIDA) to obtain a fingerprint of the macromolecular interactions on flat glassy carbon surfaces, achieved through self-organized, drop-cast,...
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Comparison of GC–MS and MEKC methods for caffeine determination in preworkout supplements
PublikacjaIn this study, GC–MS- and MEKC-based methods for determination of caffeine (CAF) in preworkout supplements were developed and validated. The proposed protocols utilized minimal sample preparation (simple dilution and syringe filtration). The developed methods achieved satisfactory validation parameters, i.e. good linearity (R2 0.9988 and R2 0.9985 for GC–MS- and MEKC-based method, respectively), satisfactory intraand interaccuracy...
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Visible light photocatalysis employing TiO2/SrTiO3-BiOI composites: Surface properties and photoexcitation mechanism
PublikacjaA series of binary TiO2/SrTiO3 and ternary TiO2/SrTiO3_BiOI composites have been synthesized using multistep preparation method. Characterization of the obtained photocatalysts covered complex analysis including morphology + y (SEM), BET specific surface area, porosity, optical properties (DRS), chemical composition of surface (XPS), crystal structure and crystal size (XRD). UV–vis and Vis ligh induced photocatalytic activity was...
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Modele mentalne przedsiębiorczości: wskaźniki efektuacyjne vs. kauzalne
PublikacjaGłównym celem artykułu było stworzenie listy wskaźników diagnozujących model mentalny przedsiębiorczości. Zaprezentowano dwa podejścia do przedsiębiorczości: klasyczne podejście kauzalne, w którym przedsiębiorca koncentruje się na osiągnięciu celu wyznaczonego na samym początku oraz podejście efektuacyjne, w którym identyfikowaniu dostępnych zasobów i relacji towarzyszy poszukiwanie możliwości biznesowych i zmienność celów. Analiza...
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Adaptacja kulturowa jako wyzwanie w zespołachmiędzynarodowych
PublikacjaWyzwania dotyczące pracy w międzykulturowym środowisku są najczęściej odnoszone do menedżerów, ze względu na powszechność ich międzynarodowych migracji oraz podejmowanie międzynarodowej współpracy z kooperantami. Rozwój globalnego rynku pracy stymuluje jednak również szeregowych pracowników do poszukiwania pracy w różnych krajach, co sprawia, że wielu z nich pracuje w międzykulturowych zespołach, gdzie doświadczają kulturowego...
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Vibration Surveillance System with Variable Stiffness Holder for Milling Flexible Details
PublikacjaEfficient milling of the flexible details (i.e. rotor blades, thin-walled elements) using slender ball-end tools is a difficult task due to possibility of vibration occurrence. Because of the existence of certain conditions (small depths of cutting, regeneration phenomena), cutting process may lose stability and self-excited chatter vibration may appear. Frequency of the chatter vibration is close to dominant natural frequency...
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‘Green’ multi - residue methods for the determination of high importance currently used pesticides in environmental samples
PublikacjaThe purpose of the research has been to develop and apply ‘green’ analytical methodologies to control and monitor the level of currently used pesticides from different chemical groups in aqueous samples. For extraction of analytes solid - phase microextraction (SPME) and dispersive liquid - liquid microextraction (DLLME) have been applied and compared. For SPME, commonly used materials for coating fibers include: polydimethylsiloxane...
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Analysis of diagnostic informativeness of the exhaust temperature of a naval gas turbine
PublikacjaThe conducted investigations aimed to carry out an analysis of the possibility that the failures identification in the automatic control system of a gas turbine engine could be worked out on the basis of measurements of the exhaust stream temperature behind the gas generator. The exhaust temperature represents a basic control parameter, enabling technical state evaluation of the engine within the operation process, which is observed...
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Detection of Toxoplasma gondii oocysts in environmental samples
PublikacjaToxoplasma gondii is an obligate intracellular parasite that has capacity to infect warm-blooded animals. Human toxoplasmosis is usually asymptomatic, whereas severe complications may occur in immunocompromised patients (e.g. AIDS) or in case of congenitalinfection. One of the sources of acquiring human toxoplasmosis is ingestion of T. gondii oocysts. It is known that newly infected cats can shed millions of oocysts into the environment,...
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New methodology for determination cationic surfactants in liquid environmental samples
PublikacjaBecause of their specific physical and chemical properties (e.g. amphiphilicity, solubility in polar and nonpolar liquids, ability to form micelles, adsorption at phase boundaries, low toxicity) surfactants (surface-active compounds) are widely applied in industry and in the household. As their applications are on a very large scale, it has become necessary to acquire a more detailed understanding of their environmental fate. Mainly,...
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Formation of chlorinated breakdown products during degradation of sunscreen agent, 2-ethylhexyl-4-methoxycinnamate in the presence of sodium hypochlorite
PublikacjaIn this study, a new degradation path of sunscreen active ingredient, 2-ethylhexyl-4-methoxycinnamate (EHMC) and 4-methoxycinnamic acid (MCA) in the presence of sodium hypochlorite (NaOCl), was discussed. The reaction products were detected using gas chromatography–mass spectrometry (GC-MS). Since HOCl treatment leads to more polar products than EHMC, application of polar extracting agents, dichloromethane and ethyl acetate/n-hexane...
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Decolorisation of Methylene Blue with Sodium Persulfate Activated with Visible Light in the Presence of Glucose and Sucrose
PublikacjaThe paper presents the results of research on the methylene blue (MB) degradation with sodium persulfate (PDS) activated with visible light (Vis) in the presence of glucose and sucrose. The following test were examined: absence and presence of sugars in concentrations of 50–300 mM, presence and absence of Vis radiation, pH of the model solution (6.0–12.0), identification of free radicals, kinetics of the pseudo-first-order reaction....
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Habitat-related patterns of soft-bottom macrofaunal assemblages in a brackish, low-diversity system (southern Baltic Sea)
PublikacjaCoastal areas provide a high variety of sedimentary habitats that influence the structure of resident fauna even on small geographical scales. Therefore, examinations of spatial variations in benthic assemblages require background knowledge of the environmental and biotic heterogeneity of habitats in order to understand ecological processes in such areas. The effect of habitat-related abiotic and biological variables on macrofaunal...
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Application of COSMO-RS-DARE as a Tool for Testing Consistency of Solubility Data: Case of Coumarin in Neat Alcohols
PublikacjaCoumarin is a naturally occurring lactone-type benzopyrone with various applications in the pharmaceutical, food, perfume, and cosmetics industries. This hydrophobic compound is poorly soluble in water but dissolves well in protic organic solvents such as alcohols. Despite the extensive use of coumarin, there are only a few reports documenting its solubility in organic solvents, and some reported data are incongruent, which...
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Fault detection in measuring systems of power plants
PublikacjaThis paper describes possibility of forming diagnostic relations based on application of the artifical neural networks (ANNs), intended for the identifying of degradation of measuring instruments used in developed power systems. As an example a steam turbine high-power plant was used. And, simulative calculations were applied to forming diagnostic neural relations. Both degradation of the measuring instruments and simultaneously...
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Application of a Gas Sensor Array to Effectiveness Monitoring of Air Contaminated with Toluene Vapors Absorption Process
PublikacjaThis article demonstrates the application of a gas sensor array to monitor the effectiveness of the absorption process of air stream purification from odorous compounds (toluene vapors). A self-constructed matrix consisting of five commercially available gas sensors was used. Multiple linear regression (MLR) was selected as the statistical technique used to calibrate the matrice. Gas chromatography coupled with a flame ionization...
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Volatile Compound Emissions from Stereolithography Three-Dimensional Printed Cured Resin Models for Biomedical Applications
PublikacjaStereolithography three-dimensional printing is used increasingly in biomedical applications to create components for use in healthcare and therapy. The exposure of patients to volatile organic compounds (VOCs) emitted from cured resins represents an element of concern in such applications. Here, we investigate the biocompatibility in relation to inhalation exposure of volatile emissions of three different cured commercial resins...
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Combination of hydrodynamic cavitation and SR-AOPs for simultaneous degradation of BTEX in water
PublikacjaHydrodynamic cavitation (HC) is an emerging technology gaining interest in water treatment for use in elimination of a wide range of organic pollutants. The energy released during cavitation phenomenon has number of applications and, particularly, it can be utilized for activation of persulfate (PS) and peroxymonosulfate (PMS). In the present study, hybrid techniques: HC combined with persulfates – HC-PS and HC-PMS were tested...
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Spatial differentiation of road safety in Europe based on NUTS-2 regions
PublikacjaRoad safety varies significantly across the regions in Europe. To understand the factors behind this differentiation and the effects they have, data covering 263 NUTS-2 (Nomenclature of Territorial Units for Statistics) regions across Europe (European Union and Norway) have been analysed. The assessment was made using Geographically Weighted Regression (GWR). As a dependent variable the Road Fatality Rate (RFR – number of fatalities...