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Spatial characterization of H 2: CH 4 dissociation level in microwave ECR plasma source by fibre-optic OES
PublikacjaSpatially resolved optical emission spectroscopy (SR-OES) was used to investigate microwave activated H2/Ar/CH4 plasma under conditions of the electron cyclotron resonance (ECR). The chemistry and composition of the gas phase were studied using self-designed fibre-optic system with echelle type spectrometer during CVD deposition of polycrystalline diamond. One-dimensional intensity profiles of the main species were collected along...
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Biological responses to environmental contamination. How can metal pollution impact signal honesty in avian species?
PublikacjaEnvironmental pollution, for example with metals, can significantly affect the ecosystem balance leading to severe changes. Biologically active pigments are relevant for the appearance and condition of birds. Melanin and carotenoid particles are the most frequently deposited pigments in avian integument. They are responsible for the majority of colors of bird plumage. The phenotypic expression can be affected by metal contamination....
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Effect of Agitation and Temporary Immersion on Growth and Synthesis of Antibacterial Phenolic Compounds in Genus Drosera
PublikacjaSundews (Drosera sp.) are the source of biologically active secondary metabolites: phenolic acids, flavonoids, and 1,4-naphtoquinones. Because obtaining them from the natural environment is impossible (rare and endangered species), in this study modifications of traditional tissue cultures grown in solid medium (SM), such as agitated cultures (ACs) (cultures in liquid medium with rotary shaking) and temporary immersion bioreactors...
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The consideration to the dynamic systems parameter identification
PublikacjaIn this paper, a concept for continuous-time dynamic systems parameter identification using modulating function approach is presented. It refers to linear as well as selected non-linear systems. It shows the possibility of direct application without converting differential equation. In particular cases direct application can decrease the amount of computation in non-linear system identification, which generally requires Fourier...
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Identification and antimicrobial resistance of Enterococcus spp. isolated from surface water
PublikacjaIn the study species distributions and antimicrobial resistance profiles were determined among riverine Enterococcus spp. Susceptibility of the isolates was tested against: ampicillin, imipenem, teicoplanin, vancomycin, erythromycin, linezolid, fosfomycin, ciprofloxacin,levofloxacin, moxifloxacin, tetracycline, high levels of gentamicin and streptomycin. The enterococci were detected in all of the analyzed water samples, periodically...
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The relationship between standard reduction potentials of catechins and biological activities involved in redox control
PublikacjaRedox homeostasis involves factors that ensure proper function of cells. The excess reactive oxygen species (ROS) leads to oxidative stress and increased risk of oxidative damage to cellular components. In contrast, upon reductive stress, insufficient ROS abundance may result in faulty cell signalling. It may be expected that dietary antioxidants, depending on their standard reduction potentials (E°), will affect both scenarios....
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Seasonal changes of mercury speciation in the coastal sediments
PublikacjaPurpose Mercury speciation in sediments is linked to environmental conditions and processes. Domination of particular mercury species depends on its source, displays considerable seasonal behavior, and may be further modified due to oxygen levels, icing conditions, or the input of fresh organic matter. The purpose of this study was to examine the coastal area of the Gulf of Gdańsk in terms of mercury contamination and the influence...
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Optimization of carbamazepine photodegradation on defective TiO2-based magnetic photocatalyst
PublikacjaIn this work, carbamazepine (CBZ) degradation over defective Fe3O4@SiO2/d-TiO2/Pt photocatalyst was studied. Within the titania structure, Ti vacancies and Pt nanoparticles were introduced to enhance the photocatalyst’s light absorption and influence charge carriers’ mobility. For the carbamazepine degradation, process parameters, e.g., temperature, flux intensity, photocatalyst loading, aeration, pH, and addition of H2O2, were optimized...
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Analyzing sets of phylogenetic trees using metrics
PublikacjaThe reconstruction of evolutionary trees is one of the primary objectives in phylogenetics. Such a tree represents historical evolutionary relationships between different species or organisms. Tree comparisons are used for multiple purposes, from unveiling the history of species to deciphering evolutionary associations among organisms and geographical areas. In this paper, we describe a general method for comparing phylogenetictrees...
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Positron binding to alkali-metal hydrides: The role of molecular vibrations
PublikacjaThe bound vibrational levels for J=0 have been computed for the series of alkali-metal hydride molecules from LiH to RbH, including NaH and KH. For all four molecules the corresponding potential-energy curves have been obtained for each isolated species and for its positron-bound complex (e+XH). It is found that the calculated positron affinity values strongly depend on the molecular vibrational state for which they are obtained...
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Biodiversity of microorganisms in the Baltic Sea: the power of novel methods in the identification of marine microbes
PublikacjaUntil recently, the data on the diversity of the entire microbial community from the Baltic Sea were relatively rare and very scarce. However, modern molecular methods have provided new insights into this field with interesting results. They can be summarized as follows. (i) Although low salinity causes a reduction in the biodiversity of multicellular species relative to the populations of the North–East Atlantic, no such reduction...
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Fast GC as a useful tool for authenticity assessment of kiwifruit
PublikacjaKiwifruit is one of the healthiest fruits due to its high content of biologically active substances and nutrients. The most popular species of Actinidia (kiwifruit) are the Actinidia deliciosa fruits called kiwi and Actinidia chinensis commonly called golden kiwi, while the lesser known species is Actinidia arguta known as mini kiwi. Bioactivities and nutrients of Actinidia are influenced by species and cultivars. It is very important...
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First report on echinocandin resistant Polish Candida isolates
PublikacjaPurpose: Candida spp. are ranked as one of the four major causative agents of fungal infections. The number of infections caused by Candida species resistant to fluconazole, which is applied as the first line drug in candidiasis treatment, increases every year. In such cases the application of echinocandin is necessary. Echinocandin susceptibility testing has become a routine laboratory practice in many countries due to the increasing...
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Characterization of GM3 Gangliosides in Human Milk throughout Lactation: Insights from the Analysis with the Use of Reversed-Phase Liquid Chromatography Coupled to Quadrupole Time-Of-Flight Mass Spectrometry
PublikacjaGangliosides are complex lipids found in human milk that play important structural and biological functions. In this study, we utilized reversed-phase liquid chromatography coupled to quadrupole time-of-flight mass spectrometry to evaluate the molecular distribution of GM3 in human milk samples collected at distinct lactation stages, ranging from colostrum to advanced lactation samples. Throughout lactation, GM3 d40:1 emerged as...
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Hierarchical MnO2 nanoflowers blooming on 3D nickel foam: A novel micro-macro catalyst for peroxymonosulfate activation
PublikacjaIn this work, birnessite-type δ-MnO2 nanoflowers were uniformly deposited on 3D nickel foam (NF) by one-step hydrothermal route for high-efficient activation of peroxymonosulfate (PMS) towards degradation of acid orange 7 (AO7). High specific surface area, large pore volume and 3D hierarchical structure promotes the mass and electron transfer for great catalytic activity. Low reaction energy barrier (Ea=27.5 kJ/mol) and outstanding...
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High-temperature Co-electrolysis of CO2/H2O and direct methanation over Co-impregnated SOEC. Bimetallic synergy between Co and Ni
PublikacjaTo study the synergy between the transition metals for enhancing the electrochemical and chemical activity, a series of SOECs were modified with a small amount of Co ions, namely 1.8, 3.6, and 5.4 wt% in the reduced state. The addition of βCD into the precursor solution allowed for extremely fine dispersion of Co species across the Ni-YSZ cermet structure. The sample containing 3.6 wt% Co reached an outstanding over 2.5-times-higher...
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Nowe stanowiska Dicranum viride (Dicranaceae) w północno-wschodniej Polsce na tle jego rozmieszczenia w województwie podlaskim
PublikacjaDicranum viride (Sull. & Lesq.) Lindb. is a rare moss species from the Dicranaceae family. It is strictly protected in Poland and is considered as a relic of primeval forests. The plant is also included in Annex II of the Natura 2000 Habitats Directive and Annex I of the Bern Convention. Its populations are threatened by habitat fragmentation and economic use of forest...
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DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation
PublikacjaA new diagnostic kit for K. oxytoca specific differentiation based on ddLMS PCR (ang. double digest ligation Mediated PCR) technique is shown. As a species-specific DNA fragment pehX gene, encoding the enzyme polygalactouronase, was chosen. The genome sequence of K. oxytoca is digested with two endonucleases: AclI and BclI which cut DNA before and after pehX gene. The polymorphic DNA fragments are ligated with AclI-end-specific...
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Species Diversity
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What should we know when choosing feather, blood, egg or preen oil as biological samples for contaminants detection? A non-lethal approach to bird sampling for PCBs, OCPs, PBDEs and PFASs
PublikacjaBirds are considered as good bio-monitors and they can provide highly valuable data about the level of contamination in their habitat. During the design of biomonitoring studies one of the first issues after choosing species is the choice of biological material. Non-lethally collected samples have recently been gaining greater attention as they offer several ethical and practical advantages. However, not all sample matrices are...
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High performance ozone nanobubbles based advanced oxidation processes (AOPs) for degradation of organic pollutants under high pollutant loading
PublikacjaAdvanced Oxidation Processes (AOPs) have proven to be an effective solution for chemical wastewater treatment, particularly for degradation of organic pollutants, especially dyes. Ozonation is recognized as one of the most prevalent AOPs. Nevertheless, some cases show a lowered efficiency of O3 utilization which is attributed to its inadequate distribution in the treated water causing low residence time, low mass transfer coefficient...
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A new dilution-enrichment sample preparation strategy for expanded metabolome monitoring of human breast milk that overcomes the simultaneous presence of low- and high-abundance lipid species
PublikacjaThe complex nature of human breast milk (HBM) makes samples difficult to analyze, requiring several extraction techniques and analytical platforms to obtain high metabolome coverage. In this work, we combined liquid-liquid extraction (LLE) and solid-phase extraction (SPE) techniques to prepare HBM samples to overcome the challenge of low- and high-abundance lipid species, enabling the semiquantitative analysis of total HBM lipids...
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Silicon’s Influence on Polyphenol and Flavonoid Profiles in Pea (Pisum sativum L.) under Cadmium Exposure in Hydroponics: A Study of Metabolomics, Extraction Efficacy, and Antimicrobial Properties of Extracts
PublikacjaThe current study aimed to investigate the impact of silicon (Si) supplementation in the form of Na2SiO3 on the metabolome of peas under normal conditions and following exposure to cadmium (Cd) stress. Si is known for its ability to enhance stress tolerance in various plant species, including the mitigation of heavy metal toxicity. Cd, a significant contaminant, poses risks to...
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Comparative analysis of mRNA transcripts of HT-29 cell line expressed in identical quantities for pathogenic E. coli strains UM146 and UM147 with control Escherichia coli Nissle 1917
PublikacjaAim of study was comparative analysis of mRNA transcripts of HT-29 cell line, expressed in identical quantities for the combination of pathogenic and non-pathogenic Escherichia coli strains. HT-29 confluent monolayers infection with two pathogenic E. coli strains UM146 and UM147 resulted in two sets of mRNA transcripts that were identical with RNA transcripts obtained for non-pathogenic one strain E. coli Nissle 1917. In this study...
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Bee Pollen and Bee Bread as a Source of Bacteria Producing Antimicrobials
PublikacjaThe principal objective of the study was the isolation and identification of bacteria that are present in mature bee bread (BB) and dried (ready for selling and consumption) bee pollen (BP). Obtained isolates were screened for their potential to inhibit select human pathogenic bacteria and their ability to produce enzymes of particular industrial importance. Four and five samples of BP and BB, respectively, were used for the study....
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Evaluation of possibilities of genotyping of Candida glabrata clinical isolates with RAPD-PCR method and microsatellite analysis
PublikacjaThe aim of the study was to compare the discriminatory power of RAPD-PCR method using RSD10 primer and microsatellite (GACA)4 analysis for genotyping clinical isolates of C. glabrata. Isolates were received from patients of two Polish hospitals: Children's Memorial Health Institute in Warsaw (n=17) and Medical University of Gdansk (n=37). Species identification was confirmed by two phenotypic methods: growth on chromagar plates...
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Novel decahedral TiO2 photocatalysts modified with Ru or Rh NPs: Insight into the mechanism
PublikacjaNovel decahedral TiO2 modified with Ru or Rh NPs were successfully synthesized by hydrothermal method. The effect of metal type as well as amount of noble metal precursor (0.05, 0.1, 0.2, 0.3 and 0.5 wt.%) on the photocatalytic activity were investigated. Decahedral TiO2 decorated by Ru and Rh nanoparticles was prepared by photodeposition method, whereas Rh doped TiO2 was obtained by onestep hydrothermal method with introduction...
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Effect of the Drying Method of Pine and Beech Wood on Fracture Toughness and Shear Yield Stress
PublikacjaThe modern wood converting processes consists of several stages and material drying belongs to the most influencing future performances of products. The procedure of drying wood is usually realized between subsequent sawing operations, affecting significantly cutting conditions and general properties of material. An alternative methodology for determination of mechanical properties (fracture toughness and shear yield stress) based...
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Ciliated protozoa in the impact zone of the Uzhgorod treatment plant
PublikacjaThis paper presents the results of studies on the Uzh River (Ukraine, Zakarpattia Oblast) near the effluent point of a sewage treatment plant in Uzhgorod. The samples were taken at various sites of the treatment plant along the stages of purification process, as well as in the river, at a number of different points above and below the wastewater discharge. At each of these objects, the temperature...
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Mercury contamination, a potential threat to the globally endangered aquatic warbler Acrocephalus paludicola
PublikacjaMercury (Hg) contamination is considered a global concern for humans and wildlife, and although the number of studies dealing with that issue continues to increase, some taxonomic groups such as small passerine birds are largely understudied. In this paper, concentration of mercury in the aquatic warbler (Acrocephalus paludicola) feathers, a globally threatened passerine species, was examined. The concentration differences between...
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Chromium (III) removal by perennial emerging macrophytes in floating treatment wetlands
PublikacjaFloating treatment wetlands (FTWs) are a sustainable solution to treat polluted water, but their role in chromium (Cr(III)) removal under neutral pH conditions remains poorly understood. This study evaluated the potential of FTWs planted with two perennial emergent macrophytes, Phragmites australis and Iris pseudacorus, to remove Cr(III) and nutrients (N and PO4-P) from water containing 7.5 mg/L TN, 1.8 mg/L PO4-P, and Cr(III)...
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Milena Marycz dr inż.
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Virulence, antifungal susceptibility and molecular mechanisms of echinocandin resistance among Candida isolates recovered from clinical specimens
PublikacjaFungi of the genus Candida belong to the natural microflora of healthy individuals. However, they can also be a cause of opportunistic infections especially among patients with an impaired immune system. The first line therapy of Candida infections is based on triazoles. However, in recent years there an increase of azole resistant Candida spp., in particular C. glabrata and C. krusei, has been observed. For this reason, echinocandin...
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Occurrence of Cyanobacteria in the Gulf of Gdańsk (2008–2009)
PublikacjaBlooms of cyanobacteria develop each summer in the Baltic Sea. Collecting complete data on this phenomenon is helpful in understanding the changes taking place in the Baltic Sea and forecasting the occurrence of these phenomena in the future. This dataset includes unpublished information about the occurrence of cyanobacteria in the Gulf of Gdańsk (Southern Baltic) in 2008 and 2009. The presented data combines basic physic-ochemical...
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VUV photofragmentation of the six-membered heterocyclic molecules containing oxygen
PublikacjaCancer is a significant public health concern worldwide, which results in millions of deaths each year. The standard cure routine for cancer is surgery, and nowadays, radiotherapy or a hadrontherapy. Depending on the type of cancer, patients may undergo additional treatment, including targeted therapy. A combination of radio- or hadron-therapy with proper drug treatment can inhibit the proliferation of cancer cells and thus can...
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Isolation and Characterization of Phenol-Degrading Psychrotolerant Yeasts
PublikacjaIn this study, the potential of selected psychrotolerant yeast strains for phenol biodegradation was studied. From 39 strains isolated from soil and water samples from Rucianka peat bog, three psychrotolerant yeast strains: A011, B021 and L012 showed the ability to phenol degradation. The result shows that all three yeast strains could degrade phenol at 500 mg l-1 and 750 mg l-1 concentration, whereas strains A011 and L012 could...
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Cuticular lipids of insects as potential biofungicides
PublikacjaThe term ''lipids'' refers to fatty acids, their derivatives and substances related with them biochemically or by the functions in the body. Lipids include compounds with different chemical structure. There are lipids with structures based on long-chain carboxylic acids and their direct derivatives, namely: fatty acids, alkanes, some alkenes, aldehydes and long chain alcohols and their derivatives (esters). The variety of lipid...
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Relationship between conversion rate of glucosinolates to isothiocyanates/indoles and genotoxicity of individual parts of Brassica vegetables
PublikacjaThe studies on the characterisation of glucosinolates (GLs) and their breakdown products in Brassicaceae species focus mainly on the edible parts. However, other products, e.g., dietary supplements, may be produced also from non-edible parts such as roots or early forms of growth: seeds or sprouts. Biological activity of these products depends on quantitative and qualitative GL composition, but is also strictly determined by GL...
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TiO 2 Co x O y composite nanotube arrays via one step electrochemical anodization for visible light–induced photocatalytic reaction
PublikacjaTiO2CoxOy (x = 1, y = 1 or x = 3, y = 4) composite photocatalysts have been synthesized via one step anodic anodization of TiCo alloys. The effects of the cobalt content in the alloy (5, 10 and 15 wt%), water content (2, 5 and 10%) in the electrolyte solution, and applied voltage (30, 40 and 50 V) during the anodization process on the morphology and surface properties. Additionally, the dependence of cobalt content in the nanotubes...
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TiO2 /SrTiO3 and SrTiO3 microspheres decorated with Rh, Ru or Pt nanoparticles: Highly UV–vis responsible photoactivity and mechanism
PublikacjaA series of TiO2/SrTiO3 and SrTiO3 microspheres decorated by Rh, Ru or Pt NPs were prepared by facile hydrothermal method. The as-prepared samples were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) with energy-dispersive Xray (EDX) spectroscopy, scanning electron microscopy (SEM), photoluminescence spectrometry (PL), Fourier transform infrared (FT-IR) and...
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Investigation of H2:CH4 plasma composition by means of spatially resolved optical spectroscopy
PublikacjaThe system based on spatially resolved optical emission spectroscopy dedicated for in situ diagnostics of plasma assisted CVD processes is presented in this paper. Measurement system coupled with chemical vapour deposition chamber by dedicated fiber-optic paths enables investigation of spatial distribution of species densities (Hx, H+, CH, CH+) during chemical vapour deposition process. Experiments were performed for a various...
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Reactivity of bulky aminophosphanes towards small molecules: Activation of dihydrogen and carbon dioxide by aminophosphane/borane frustrated Lewis pairs
PublikacjaA series of mono- and bisaminophosphanes with formulas R2NPR’R’’ and (R2N)2PR’ (R = iPr, Cy; R’ = Ph, Cy; R’’ = iPr) were characterized by X-ray analysis, NMR spectroscopy and computational methods. The common structural futures of these species are short and polarized PAN bonds, a pyramidal geometry at the P atom and an almost planar geometry at the N atoms. According to DFT calculations, these...
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Classification and fingerprinting of kiwi and pomelo fruits by multivariate analysis of chromatographic and spectroscopic data
PublikacjaThe fingerprinting capacity of two analytical techniques, HPLC and UV-Vis spectroscopy in the case of fruits samples (kiwi and pomelo) has been investigated. The multivariate exploratory techniques were used for sample discrimination according to the fruit species and subspecies. The combination of principal component analysis with linear discriminant analysis is opening new directions in the fingerprinting analysis. The classifications...
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Speciation of Iron in the Aquatic Environment
PublikacjaIron is one of the basic metals occurring in the aquatic environment and it is considered a macroelement with regard to live organisms. This metal has a broad range of applications that, together with factors conditioning its chemical transitions, results in the occurrence of many iron species in water. Depending on water and land development type, as well as the use of water for household and industrial purposes, iron may be found...
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New locations, distribution and protection of the red helleborine Cephalanthera rubra (L.) Rich. (Orchidaceae) in the Augustów Primeval Forest (NE Poland)
PublikacjaCephalanthera rubra (L.) Rich. is a species under strict protection, very rare in the Suwałki region. In recent years, two sites were discovered in the Wigry National Park. The article presents a description of new localities and the distribution of the red helleborine in the Augustów Primeval Forest.
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Serodiagnosis of Toxoplasma gondii infection in farm animals (horses, swine, and sheep) by enzyme-linked immunosorbent assay using chimeric antigens
PublikacjaToxoplasma gondii infects all warm-blooded animals including humans, causing serious public health problems and great economic loss in the animal husbandry. Commonly used serological tests for diagnosis of toxoplasmosis involve preparation of whole Toxoplasma lysate antigen (TLA) from tachyzoites. The production of this antigen is associated with high costs and lengthy preparation and the possibility of staff infection. There are...
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An experimental assessment on a diesel engine powered by blends of waste-plastic-derived pyrolysis oil with diesel
PublikacjaThe utilization of plastic solid wastes for sustainable energy production is a crucial aspect of the circular economy. This study focuses on pyrolysis as an effective method to convert this feedstock into renewable drop-in fuel. To achieve this, it is essential to have a comprehensive understanding of feedstock composition, pyrolysis process parameters, and the physicochemical characteristics of the resulting fuel, all correlated...
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High-Temperature Oxidation of Heavy Boron-Doped Diamond Electrodes: Microstructural and Electrochemical Performance Modification
PublikacjaIn this work, we reveal in detail the effects of high-temperature treatment in air at 600 °C on the microstructure as well as the physico-chemical and electrochemical properties of boron-doped diamond (BDD) electrodes. The thermal treatment of freshly grown BDD electrodes was applied, resulting in permanent structural modifications of surface depending on the exposure time. High temperature affects material corrosion, inducing...
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High-performance activation of ozone by sonocavitation for BTEX degradation in water
PublikacjaThis work presents a novel advanced oxidation process (AOP) for degradation of emerging organic pollutants – benzene, toluene, ethylbenzene and xylenes (BTEXs) in water. A comparative study was performed for sonocavitation assisted ozonation under 40–120 kHz and 80–200 kHz dual frequency ultrasounds (DFUS). Based on the obtained results, the combination of 40–120 kHz i.e., low-frequency US (LFDUS) with O3 exhibited excellent oxidation...
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Microbial diversity of inflamed and noninflamed gut biopsy tissues in inflammatory bowel disease.
PublikacjaBACKGROUND: Inflammatory bowel disease (IBD) is a chronic gastrointestinal condition without any known cause or cure. An imbalance in normal gut biota has been identified as an important factor in the inflammatory process. METHODS: Fifty-eight biopsies from Crohn's disease (CD, n = 10), ulcerative colitis (UC, n = 15), and healthy controls (n = 16) were taken from a population-based case-control study. Automated ribosomal intergenic...