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A new cold-adapted beta-D-galactosidase from the Antarctic Arthrobacter sp. 32c - gene cloning, overexpression, purification and properties
PublikacjaThe development of a new cold-active β-D-galactosidases and microorganisms that efficiently ferment lactose is of high biotechnological interest, particularly for lactose removal in milk and dairy products at low temperatures and for cheese whey bioremediation processes with simultaneous bio-ethanol production. In this article, we present a new β-D-galactosidase as a candidate to be applied in the above mentioned biotechnological...
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Statistical radii associated with amino acids to determine the contact map: fixing the structure of a type I cohesin domain in theClostridium thermocellumcellulosome
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Excess volumes of mixing in (N,N-Dimethylacetamide+methanol+water) and (N,N-Dimethylacetamide+ethanol+water) at the temperature 313.15 K
PublikacjaW pracy podane są rezultaty precyzyjnych pomiarów namiarowych objętości molowych dla dwóch układów trójskładnikowych zawierających wodę, amid alifatyczny (N,N-dimetyloacetamid) oraz alkohol: metanol lub etanol. Dla wszystkich badanych układów obserwowane są duże i ujemne wartości nadmiarowych objętości mieszania, wskazujące na obecność silnych oddziaływań międzycząsteczkowych.
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Joining of tubular elements by internal rolling with use of burnishing tools.Łączenie elementów rurowych przez wewnętrzne rozpęczanie z użyciem nagniataków.
PublikacjaW referacie przedstawiono wyniki prac zmierzających do zastosowania nagniataków do łączenia rur aluminiowych przez ich rozgniatanie. Zaprezentowano analityczny szacunek wytrzymałości złącz i porównano z wynikami prób ich rozłączania na maszynie wytrzymałościowej.
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Structure-function analysis of decay-accelerating factor: identification ofresidues important for binding of the Escherichia coli Dr adhesin and complement regulation
PublikacjaZidentyfikowano ważne dla wiązania adhezyny Dr uropatogennych Escherichiacoli reszty aminokwasowe w ludzkim receptorze DAF SCR-3.
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Towards evaluation of the Second Generation Intact Stability Criteria - examination of a fishing vessel vulnerability to surf-riding, based on historical capsizing
PublikacjaThe Second Generation Intact Stability Criteria (SGISC) are the latest achievements in the field of intact ship stability assessment. The criteria are the result of massive international cooperative efforts and extensive research that lasted more than 15 years. Even though they are based on a solid scientific foundation, the trial period has commenced to collect user experience and further validation. One of possible approaches...
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How do sterols determine the antufungal activitiy of amphotericin B? Free energy of binding between the drug and its membrane targets.
PublikacjaAmphotericin B (AmB) is a well-known polyene antibiotic used to treat systemic fungal infections. It is commonly accepted that the presence of sterols in the membrane is essential for the AmB biological activity, that is, for the formation of transmembrane ion channels. The selective toxicity of AmB for fungal cells is attributed to the fact that it is more potent against fungal cell membranes containing ergosterol than against...
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The influence of combination of binding agents on fatigue properties of deep cold in-place recycled mixtures in Indirect Tensile Fatigue Test (ITFT)
PublikacjaThe publication presents fatigue properties of cold recycled mixtures for eight combinations of binding agents (cement and bituminous emulsion). Cold recycled mixtures were evaluated in Indirect Tensile Fatigue Test (ITFT) at the temperature of 20 C in controlled stress mode. As a function of horizontal stress, fatigue life is strongly influenced by combination of the binding agents. When fatigue life is analyzed as a function...
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THE GENE CLONING, OVEREXPRESSION, PURIFICATION AND BIOCHEMICAL CHARACTERISATION OF A NEW COLD-ADAPTED β–GALACTOSIDASE FROM ARTHROBACTER SP. VII-4
PublikacjaINTRODUCTION. β-Galactosidase [EC 3.2.1.23] is an enzyme that catalyzes the hydrolysis of O-glycosidic linkages in galactosides. It is commercially used in dairy industry for the production of milk with reduced lactose content. Potentially, the best method for lactose removal under cooling conditions should be carried out with a cold-adapted enzyme. AIM. The aim of this study was to determine the taxonomic affiliation of the isolate...
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Iso-Partricin, an Aromatic Analogue of Amphotericin B: How Shining Light on Old Drugs Might Help Create New Ones
PublikacjaPartricin is a heptaene macrolide antibiotic complex that exhibits exceptional antifungal activity, yet poor selective toxicity, in the pathogen/host system. It consists of two compounds, namely partricin A and B, and both of these molecules incorporate two cis-type bonds within their heptaenic chromophores: 28Z and 30Z. In this contribution, we have proven that partricins are susceptible to a chromophore-straightening photoisomerization...
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Algebraic periods and minimal number of periodic points for smooth self-maps of 1-connected 4-manifolds with definite intersection forms
PublikacjaLet M be a closed 1-connected smooth 4-manifolds, and let r be a non-negative integer. We study the problem of finding minimal number of r-periodic points in the smooth homotopy class of a given map f: M-->M. This task is related to determining a topological invariant D^4_r[f], defined in Graff and Jezierski (Forum Math 21(3):491–509, 2009), expressed in terms of Lefschetz numbers of iterations and local fixed point indices of...
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Reducing the number of periodic points in the smooth homotopy class of a self-map of a simply-connected manifold with periodic sequence of Lefschetz numbers
PublikacjaLet f be a smooth self-map of an m-dimensional (m >3) closed connected and simply-connected manifold such that the sequence of the Lefschetz num- bers of its iterations is periodic. For a fixed natural r we wish to minimize, in the smooth homotopy class, the number of periodic points with periods less than or equal to r. The resulting number is given by a topological invariant J[f] which is defned in combinatorial terms and is...
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School psychologist’s interventions to promote successful school re-entry of chronically ill children aiming at altering peers’ attitudes towards them
PublikacjaThe aim of this article is to outline school psychologist’s interventions, which might be useful while supporting a chronically, somatically ill child in their re-entry to school, aiming at broadening their peers’ knowledge about the chronic disease and positively changing their attitudes towards the ill child. In the first part, the importance of peer relationships in childhood and adolescence, the relation of their quality...
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Multiple intermodal-vectorial four-wave mixing bands generated by selective excitation of orthogonally polarized LP01 and LP11 modes in a birefringent fiber
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Probing the binding selected metal ions and biologically active substances to the antimicrobial peptide LL-37 using DSC, ITC measurements and calculations
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On the rising extra storage capacity of ultra-small Fe3O4particles functionalized with HCS and their potential as high-performance anode material for electrochemical energy storage
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Badania teoretyczne łożysk ślizgowych podpartych na czaszy kulistej = Theoretical tests of hydrodynamic journal bearing with self-aligning spherical support
PublikacjaPrzedstawiono metodykę badań teoretycznych i doświadczalnych łożysk ślizgowych podpartych na czaszy kulistej. Badania teoretyczne prowadzono z wykorzystaniem programu komputerowego MES oraz programu wyznaczającego charakterystyki filmu olejowego. W celu opisu systemu tribologicznego łożysko-podpora stworzono trójwymiarowy model MES łożyska i gniazda. W czasie badań analizowano wpływ wielu czynników mogących ułatwiać lub utrudniać...
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A new B-D-galactosidase with a low temperature optimum isolated from the Antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization.
PublikacjaA psychrotrophic bacterium producing a coldadaptedB-galactosidase upon growth at low temperatureswas classiWed as Arthrobacter sp. 20B. A genomic DNAlibrary of strain 20B introduced into Escherichia coliTOP10F' and screening on X-Gal (5-bromo-4-chloro-3-indolyl-B-D-galactopyranoside)-containing agar plates ledto the isolation of B-galactosidase gene. The B-galactosidasegene (bgaS) encoding a protein of 1,053 amino acids,with a...
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Analysis of interactions responsible for vasopressin binding to human neurohypophyseal hormone receptors—molecular dynamics study of the activated receptor–vasopressin–Gα systems
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Enormously large (approaching 14 eV!) electron binding energies of [HnFn+1]- (n= 1–5, 7, 9, 12) anions
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The extended model predictive-sliding mode control of three-level AC/DC power converters with output voltage and load resistance variations
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Recycling of Industrial Waste as Soil Binding Additives—Effects on Soil Mechanical and Hydraulic Properties during Its Stabilisation before Road Construction
PublikacjaTo improve the in situ soil stabilization, different chemical additives are used (ion exchange compounds, additives based on H2SO4 or vinyl polymers, and organic additives using lignosul- fonates). One interesting alternative is the production of additives from various waste materials. The extensive testing of waste-based blends with soil was performed; the mechanical (unconfined compressive strength (UCS)) and hydraulic (capillary...
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A novel cold-active β-D-galactosidase with transglycosylation activity from the Antarctic Arthrobacter sp. 32cB - gene cloning, purification and characterization
PublikacjaA gene encoding a novel β-D-galactosidase from the psychrotolerant Antarctic bacterium Arthrobacter sp. 32cB was isolated, cloned and expressed in Escherichia coli. The active form of recombinant β-D-galactosidase consists of two subunits with a combined molecular weight of approximately 257 kDa. The enzyme's maximum activity towards o-nitrophenyl-β-D-galactopyranoside was determined as occurring at 28 °C and pH 8.0. However, it...
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Influence of the Grain Size Distribution of the Limestone Additives on the Color Properties and Phase Composition of Sintered Ceramic Materials Based on Cream-Firing Clays
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Cloning and characterization of a novel cold-active glycoside hydrolase family 1 enzyme with beta-glucosidase, beta-fucosidase and beta-galactosidase activities.
PublikacjaBackground: Cold-active enzymes, sourced from cold-adapted organisms, are characterized by high catalytic efficiencies at low temperatures compared with their mesophilic counterparts, which have poor activity. This property makes them advantageous for biotechnology applications as it: (i) saves energy costs, (ii) shortens the times for processes operated at low temperatures, (iii) protects thermosensitive substrates or products...
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Flexible Knowledge–Vision–Integration Platform for Personal Protective Equipment Detection and Classification Using Hierarchical Convolutional Neural Networks and Active Leaning
PublikacjaThis work is part of an effort to develop of a Knowledge-Vision Integration Platform for Hazard Control (KVIP-HC) in industrial workplaces, adaptable to a wide range of industrial environments. The paper focuses on hazards resulted from the non-use of personal protective equipment (PPE). The objective is to test the capability of the platform to adapt to different industrial environments by simulating the process of randomly selecting...
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Mechanism of Binding of Antifungal Antibiotic Amphotericin B to Lipid Membranes: An Insight from Combined Single-Membrane Imaging, Microspectroscopy, and Molecular Dynamics
PublikacjaAmphotericin B is a lifesaving polyene antibiotic used in the treatment of systemic mycoses. Unfortunately, the pharmacological applicability of this drug is limited because of its severe toxic side effects. At the same time, the lack of a well-defined mechanism of selectivity hampers the efforts to rationally design safer derivatives. As the drug primarily targets the biomembranes of both fungi and humans, new insights into the...
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Activation of N2O and SO2 by the P–B Bond System. Reversible Binding of SO2 by the P–O–B Geminal Frustrated Lewis Pair
PublikacjaHerein, we present the first transformation of borylphosphine into borylphosphinite using nitrous oxide. Borylphosphine reacts with N2O via insertion of a single oxygen atom into the P−B bond and formation of a P−O−B bond system. Borylphosphine and borylphosphinite capture SO2 and activate it in an irreversible and reversible manner, respectively.
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THE ANALYSIS OF THE INFLUENCE OF STRESS DISTRIBUTION ON WEAR PROFILE IN LUBRICATED SLIDING CONTACT OF UHMW-PE VS TITANIUM Ti-13Nb-13Zr ALLOY
PublikacjaMetal – polymer sliding contacts are a typical combination in industry and medicine. For decades such a set of materials has been the primary choice in human joints endoprosthetic technology. In this paper tribological issues of are presented from a research on the potential for practical use of Ti-13Nb-13Zr/UHMW-PE couple for orthopedic endoprosthesis. In tests on simplified models it is critically important to carefully...
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Comparative wear test of journal sliding bearings with sintered bronze and Babbitt alloy bushes lubricated by environmentally acceptable/adapted lubricants (EAL)
PublikacjaA growing awareness of the negative effects of mineral oils on the natural environment has resulted in the introduction of new regulations related to environmental protection. One of these regulations requires the use of environmentally acceptable/adapted lubricants (EAL) to lubricate marine main shaft bearings, in place of the mineral lubricating oils that have been used for decades. Classification Societies, which supervise...
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Methods of mapping out the route contour of a railway line on the measured rail irregularities
PublikacjaStosowane obecnie toromierze elektroniczne wykorzystywane są przede wszystkim do pomiaru geometrii toru. Dane z pomiarów nierówności mogą być również wykorzystane w procesie projektowania regulacji toru (np. podczas usuwania dołków). W mongrafii przedstawiono metodę wyznaczenie profilu podłużnego linii kolejowej na podstawie pomierzonych nierówności pionowych. Wyznaczony w ten sposób profil w dalszym etapie wykorzystany by był...
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Investigations of the isolators containing microstrip FCL section
PublikacjaW pracy opisany został projekt układu izolatora zawierającego podłużnie magnesowane ferrytowe sprzężone linie mikropaskowe (FCL). W warstwowej strukturze układu ferryt został wprowadzony jako nakładka. Obciążenie w układzie izolatora zostało zrealizowane na dwa sposoby: jako obciążenie współosiowe oraz jako mikopaskowy układ planarny zawierający skupiony rezystor. Uzyskane z symulacji wyniki zostały zweryfikowane eksperymentalnie....
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PM10 concentration and microbiological assessment of air in relation to the number of acute cases of type 1 diabetes mellitus in the Lubelskie Voivodeship. Preliminary report
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Phenolic Acid Content and Antioxidant Properties of Edible Potato (Solanum tuberosum L.) with Various Tuber Flesh Colours
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Does the community-based combined Meeting Center Support Programme (MCSP) make the pathway to day-care activities easier for people living with dementia? A comparison before and after implementation of MCSP in three European countries
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Conformational latitude – activity relationship of KPPR tetrapeptide analogues toward their ability to inhibit binding of vascular endothelial growth factor 165 to neuropilin‐1
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Lower rim substituted p-tert-butylcalix[4]arene; Part 14. Synthesis, structures and binding studies of calix[4]arene thioamides
PublikacjaA number of p-tert-butylcalix[4]arene thioamides were synthesized and characterized by 1H-NMR and elemental analysis. Compounds 1-5 are O-substituted derivatives with -CH2-C(=S)-N-X groups, where NX = morpholidyl, NEt2, NHC2H4Ph, NHCH2Ph and NHEt,respectively. The X-ray structures of the ligands 1, 3, 5 and of the complex 3Pb(ClO4)2, (compound 6), are presented and their slightly distorted cone conformation is established. The...
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Cloud-to-ground lightning dipole moment from simultaneous observations by ELF receiver and combined direction finding and time-of-arrival lightning detection system
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Effect of mixing method and particle size on hardness and compressive strength of aluminium based metal matrix composite prepared through powder metallurgy route
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Inhibition of inflammatory response in human keratinocytes by magnetic nanoparticles functionalized with PBP10 peptide derived from the PIP2-binding site of human plasma gelsolin
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Design, Chemical Synthesis and Kinetic Studies of Trypsin Chromogenic Substrates Based on the Proteinase Binding Loop of Cucurbita maxima Trypsin Inhibitor (CMTI-III)
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Modelling of SO2 and NOx Emissions from Coal and Biomass Combustion in Air-Firing, Oxyfuel, iG-CLC, and CLOU Conditions by Fuzzy Logic Approach
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Dynamics of changes in iron concentration and total iron binding capacity in blood plasma of goat kids during their first month of life (Short Communication)
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Single and Dual-GPU Generalized Sparse Eigenvalue Solvers for Finding a Few Low-Order Resonances of a Microwave Cavity Using the Finite-Element Method
PublikacjaThis paper presents two fast generalized eigenvalue solvers for sparse symmetric matrices that arise when electromagnetic cavity resonances are investigated using the higher-order finite element method (FEM). To find a few loworder resonances, the locally optimal block preconditioned conjugate gradient (LOBPCG) algorithm with null-space deflation is applied. The computations are expedited by using one or two graphical processing...
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Exploring thiophene-2-acetate and thiophene-3-acetate binding modes towards the molecular and supramolecular structures and photoluminescence properties of Pb(ii) polymers
PublikacjaTo evaluate the impact of the flexible positional isomeric ligands thiophene-2-acetate (2tpacCOO) andthiophene-3-acetate (3tpacCOO) on the construction and self-assembly process of Pb(II) polymers, twonovel compounds, [Pb(2tpacCOO)2(H2O)]n(1) and [Pb(3tpacCOO)2]n(2), were preparedviaanonhydro-thermal method with respect to green chemistry rules. The obtained polymers were fully characterized byelemental analysis, TG/DTG and PXRD,...
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The Product of Matrix Metalloproteinase Cleavage of Doxorubicin Conjugate for Anticancer Drug Delivery: Calorimetric, Spectroscopic, and Molecular Dynamics Studies on Peptide–Doxorubicin Binding to DNA
PublikacjaMatrix metalloproteinases (MMPs) are extracellular matrix degradation factors, promoting cancer progression. Hence, they could provide an enzyme-assisted delivery of doxorubicin (DOX) in cancer treatment. In the current study, the intercalation process of DOX and tetrapeptide-DOX, the product of the MMPs' cleavage of carrier-linked DOX, into dsDNA was investigated using stationary and time-resolved fluorescence spectroscopy, UV-Vis...
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Simultaneous removal of heavy metals and dyes in water using a MgO-coated Fe3O4 nanocomposite: Role of micro-mixing effect induced by bubble generation
PublikacjaThis study focused on the development of a nano-adsorbent for contaminant removal without the use of any external energy. An eco-friendly Fe3O4@MgO core-shell nanocomposite was synthesized and tested for the removal of a heavy metal, lead (Pb2+) and a dye, rhodamine B (RhB). The addition of H2O2 into the system enabled the self-mixing of the aqueous solution containing Fe3O4@MgO through the generation of bubbles. This system showed...
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An insight into the mixed quantum mechanical-molecular dynamics simulation of a ZnII-Curcumin complex with a chosen DNA sequence that supports experimental DNA binding investigations
PublikacjaAn important aspect of research pertaining to Curcumin (HCur) is the need to arrest its degradation in aqueous solution and in biological milieu. This may be achieved through complex formation with metal ions. For this reason, a complex of HCur was prepared with ZnII, that is not likely to be active in redox pathways, minimizing further complications. The complex is monomeric, tetrahedral, with one HCur, an acetate and a molecule...
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Simultaneous voltammetric determination of Cd2+, Pb2+, and Cu2+ ions captured by Fe3O4@SiO2 core-shell nanostructures of various outer amino chain length
PublikacjaIn the present study, we examined a novel functionalised magnetic nanoparticles Fe3O4@SiO2-Nn as a nano adsorbent for binding of Cd2+, Pb2+, Cu2+ ions in an aqueous solution. First, we obtained the nanoparticles functionalised with various carbon chains containing different number of amino groups: (3-amino)propyltriethoxysilane (Fe3O4@SiO2-N1), N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (Fe3O4@SiO2-N2) and N1-(3-trimethoxysilylpropyl)diethylenetriamine...
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Cross-talk modeling in coupled transmission lines terminated with nonlinear loads
PublikacjaW pracy przedstawiono przegląd dostępnych metod symulacji zjawiska przesłuchu kładąc szczegółny nacisk na efekty nieliniowości rzeczywistych obciążeń linii sygnałowych na płytach drukowanych. Wykorzystując metodę analizy opartą o szeregi Volterry przeanalizowano efekt przesłuchu w strukturze sprzężonych linii mikropaskowych obciążonych na wyjściu toru transmisyjnego diodą Schottky'ego z niską barierą potencjalu. Uzyskano bardzo...