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Immobilization on magnetic nanoparticles of therecombinant trehalose synthase from Deinococcusgeothermalis
PublikacjatIn our study the gene encoding trehalose synthase from Deinococcus geothermalis was cloned and overexpressed inEscherichia coli Rosetta (DE3)pLysS. Wild-type trehalose synthase has been purified from host protein after cell dis-ruption and precipitation at 20% ammonium sulphate saturation. Recombinant trehalose synthase was immobilizedonto glutaraldehyde activated silanized magnetic ferrous-ferric oxide by using covalent binding...
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Isolation and properties of recombinant trehalose synthase
PublikacjaBiosynthesis of trehalose is carried out via various matabolic pathways, including the use of trehalose synthase. Bacteria of the genus Deionococcus are microorganisms which produce trehalose synthase catalizing conversion of maltose into trehalose. In this work recombinant trehalose synthase from D.radiodurans was characterized.
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Properties of recombinant trehalose synthase from Deinococcus radiodurans expressed in Escherichia coli
PublikacjaA trehalose synthase gene from Deinococcus radiodurans(DSMZ 20539)containing 1659 bp reading frame encoding 552 amino acids was amplified using PCR. The gene was finally ligated into pET30Ek/LIC vector and expressed after isopropyl β-d-thiogalactopyranoside induction in Escherichia coli (DE3) Rosetta pLysS. The recombinanttrehalose synthase (DraTreS) containing a His6-tag at the C-terminus was purified by metal affinity chromatography...
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Effects of the polyhistidine tag on kinetics and other properties of trehalose synthase from Deionococcus geothermalis
PublikacjaTwo recombinant trehalose synthases from Deinococcus geothermalis (DSMZ 11300) were compared. A significant influence of the artificial polyhistidine tag was observed in protein constitution. The recombinant trehalose synthase from D. geothermalis with His6 -tag has a higher K m value of 254 mM, in comparison with the wild-type trehalose synthase (K m 170 mM), and displayed a lower activity of maltose conversion when compared...
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Smart Grid in Practise - Implementation of the Billaterall Supply in Gdynia Trolleybus Network
PublikacjaSince 2001, trolleybus system in Gdynia has been involved in many activities related to the reduction of power consumption, both in terms of implementation and research and development. In 2011 was introduced the first supercapacitor storage energy system. In PKT started applications of Smart Grid technologies in supply network: the bilateral supply. The paper presents results of these this novel investitions.
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Analiza kosztów i czasu budowy domu jednorodzinnego w technologii drewna krzyżowo klejonego CLT
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Prenatal Clinical Assessment of NT-proBNP as a Diagnostic Tool for Preeclampsia, Gestational Hypertension and Gestational Diabetes Mellitus
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An Experimental and Numerical Analysis of Water Hammer Phenomenon in Slurries
PublikacjaThe analysis of slurry transportation in pressure pipelines is important both from practical and theoretical point of view. Due to the nature of the medium, the number of problems arising in the course of design, operation, measurements and mathematical modeling is much higher compared to the cases where the flowing liquid is homogeneous. The equations describing the flow are more complex, and higher number of the parameters is...
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Bioactive polyurethanes applied as a surgical implants
PublikacjaSynthetic materials are applied in many branches of the industry, i.a. in medicine as a casing of apparatus, elements of artificial organs (hearts, blood vessels), catheters, wound healings, intra aortic balloons, mammary implants to mention but a few. Polymeric material used in such applications must be distinguished by a good biocompatybility. Since many years extensive research are employed to develop a new polymers that can...
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Facilitated water transport in composite reduced graphene oxide pervaporation membranes for ethanol upgrading
PublikacjaHigh purity ethanol is one of the most sought-after renewable energy sources. However, standard production methods yield ethanol of insufficient quality. Membrane processes such as pervaporation are recognized as a viable method for upgrading ethanol. Their performance and selectivity depend solely on membrane employed. Hydrophilic polyvinyl alcohol (PVA) membranes are used industrially for this purpose, but there is a trade-off...
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The Role of Hypoxia-Inducible Factor-1α, Glucose Transporter-1, (GLUT-1) and Carbon Anhydrase IX in Endometrial Cancer Patients
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Palladium versus Platinum: The Metal in the Catalytic Center of a Molecular Photocatalyst Determines the Mechanism of the Hydrogen Production with Visible Light
PublikacjaTo develop highly efficient molecular photocatalysts for visible light-driven hydrogen production, a thorough understanding of the photophysical and chemical processes in the photocatalyst is of vital importance. In this context, in situ X-ray absorption spectroscopic (XAS) investigations show that the nature of the catalytically active metal center in a (N^N)MCl2 (M=Pd or Pt) coordination sphere has a significant impact on the...
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Innovative cold-formed GEB section under compression
PublikacjaCold-formed steel sections extensively affect the modern steel construction industry. Not only are they used as secondary elements like purlins or sheeting, but also they can serve as primary load-bearing members in fabricated steel panels and trusses. A new cross-section called shortly a GEB may serve as that member. However, its existence of the metal building structures industry depends on the exact, optimal dimensional parameters...
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Stability and load bearing capacity of a braced truss under upward wind loading
PublikacjaThe paper is focused on the numerical and experimental investigation of stability of a steel truss under upward wind loading. The structure was stiffened by elastic braces situated at the top and bottom chord. Usually the lateral (translational) brace stiffness is considered. However, the rotational stiffness of braces caused by interaction between torsional stiffness of the truss top chord and bending stiffness of the roof elements...
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Stability and load bearing capacity of a braced truss under upward wind loading
PublikacjaThe paper is focused on the numerical and experimental investigation of stability of a steel truss under upward wind loading. The structure was stiffened by elastic braces situated at the top and bottom chord. Usually the lateral (translational) brace stiffness is considered. However, the rotational stiffness of braces caused by interaction between torsional stiffness of the truss top chord and bending stiffness of the roof elements...
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Komputerowa technika pomiarowa : oprogramowanie wirtualnych przyrządów pomiarowych w LabVIEW
PublikacjaKsiążkę rozpoczyna omówienie pojęcia przyrządu wirtualnego, struktury przyrządów i ich klasyfikacji. Podano przykłady przyrządów wirtualnych, stanowiących zarówno odpowiedniki przyrządów rzeczywistych, jak i nietypowych, do specjalistycznego zastosowania. Następnie dokonano przeglądu metod programowania przyrządów wirtualnych, m.in. z użyciem środowisk LabWindows/CVI, TestPoint i DASYLab. Zasadniczą część książki zajmuje szczegółowy...
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Design and Performance Evaluation of the Energy Subsystem of a Hybrid Light andWave Energy Harvester
PublikacjaThe paper presents the design and performance of an energy subsystem (ES) dedicated to hybrid energy harvesters (HEHs): wave energy converters (WECs) combined with photovoltaic panels (PVPs). The considered ES is intended for compact HEHs powering autonomous end-node devices in distributed IoT networks. The designed ES was tested experimentally and evaluated in relation to the mobile and wireless distributed communication use case....
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Advances in Modelling and Analysis of Strength of Corroded Ship Structures
PublikacjaThe present study reviews the recent advances in modelling and analyses the strength of corroded ship structures. Firstly, the time-variant methodologies that consider only the mean structural element thickness loss due to corrosion degradation are identified. Corrosion degradation is regarded as the phenomenon that causes uneven thinning of specimens. This has been captured by various researchers as the loss of mechanical properties...
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Low-Voltage Low-Power Filters with Independent ω0 and Q Tuning for Electronic Cochlea Applications
PublikacjaAn acoustic second-order low-pass filter is proposed for filter banks emulating the operation of a human cochlea. By using a special filter structure and an innovative quality (Q)-factor tuning technique, an independent change of the cutoff frequency (ω0) and the Q-factor with unchanged gain at low frequencies is achieved in this filter. The techniques applied result in a simple filter design with low Q-factor sensitivity to component...
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Biochemical assays of intensified methane content in biogas from low-temperature processing of waste activated sludge
PublikacjaAnaerobic digestion (AD) is implemented as an important ‘waste to energy’ approach for converting organic-rich byproducts such as sewage sludge into biogas and nutrient-rich digestate. Sewage sludge consists of primary sludge and waste activated sludge (WAS), and low biodegradability of WAS limits methane production. This study presents the influence of the low-temperature pretreatment (LT-PT) of WAS on the efficiency of AD. Different...
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ENERGY SAVINGS BY APPLICATION OF SUPERCAPACITOR STORAGE IN TROLLEYBUS SUPPLYING STATION – ANALYSIS OF EXPERIMENTAL RESULTS
PublikacjaIn 2011–2013, stationary supercapacitor storage developed by the Electrotechnical Institute in Warsaw was put into trial operation in the trolleybus substation “Północna” (North) located in Gdynia. The article presents the results recorded during supercapacitor storage operation and discusses the impact of the storage on energy consumption savings. Guidelines for selecting a location for stationary supercapacitor storage...
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One-step formation of n-alkenyl-malonamides and n-alkenyl-thiomalonamides from carbamoyl meldrum's acids.
PublikacjaA one-pot synthesis for the preparation of N-alkenyl-malonamides and N-alkenyl-thiomalonamides was developed. 5-[Hydroxy/mercapto(aryl/alkylamino)methylene]-2,2-dimethyl-1,3-dioxane-4,6-dione act as a source of ketenes that react with the tautomeric form of alkyl-(2-phenyl-propylidene)-amines. A possible [2+2] or [4+2] cycloaddition product of ketene to imines was not observed.
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Ocena zdatności hydraulicznych zespołów sterujących uruchamianych w niskich temperaturach otoczenia
PublikacjaPrzedstawiono wyniki badań eksperymentalnych i modelowych zespołów sterujących układów hydraulicznych (zawór przelewowy UZPP16 firmy PONAR WADOWICE, rozdzielacze 4WEH16C33/6AW220-50 firmy REXROTH i RE2510/101 firmy HYDROTOR, rozdzielacz proporcjonalny PVG 32 firmy SAUER DANFOSS, serwozawór 4WS2EM10 - 45/20B2T315Z8EM firmy REXROTH) w niskich temperaturach otoczenia
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Impact of TiO2 Nanostructures on Dye-Sensitized Solar Cells Performance
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Simulating Power Generation from Photovoltaics in the Polish Power System Based on Ground Meteorological Measurements—First Tests Based on Transmission System Operator Data
PublikacjaThe Polish power system is undergoing a slow process of transformation from coal to one that is renewables dominated. Although coal will remain a fundamental fuel in the coming years, the recent upsurge in installed capacity of photovoltaic (PV) systems should draw significant attention. Owning to the fact that the Polish Transmission System Operator recently published the PV hourly generation time series in this article, we aim...
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Długie pociągi
PublikacjaWskazano, że przy wprowadzaniu długich pociągów pojawiają się możliwości, ale i ograniczenia różnej natury oraz problemy do rozwiązania. Inne w przypadku długich pociągów i inne w przypadku ciężkich pociągów. Pokazano długie pociągi, jako szansę na polepszenie bezpieczeństwa na drogach, poprzez ograniczenie na nich ruchu samochodów ciężarowych. Łatwiej będzie w praktyce realizować pomysł „Tiry na tory” Długie pociągi są szansą...
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Endocrine Disrupting Compounds – Problems and Challenges
PublikacjaIn this chapter, information about some of the estrogenic compounds and their environmental fate and biological influence can be found. Special attention is paid to the review of the analytical approaches used at the stages of detection and determination of Endocrine Disrupting Compounds (EDCs) in the environmental samples. Also, a brief characterization of both cellular and non-cellular bioassays is presented. The discovery of...
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Novel Biodegradable Potato Starch-based Compositions as Candidates in Packaging Industry, Safe for Marine Environment
PublikacjaAbout 70 % of our planet’s surface is covered by seas and oceans to which even 10 million tons of waste go every year. It makes these places the largest global landfills, containing up to 90 % of plastic waste. In this article we present the results of research on novel starch-based compositions expected to be more safe for the marine environment. For these purpose biopolymers such as, thermoplastic starch (TPS), polylactide (PLA)...
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Selected issues on the role of electric lighting in the regeneration programs of urban spaces in Poland
PublikacjaThis paper reflects on the basic research done in the area of outdoor public lighting in regard to regeneration processes. The general purpose of this scientific paper is to investigate the role that lighting design plays within regeneration frameworks. Referring to a wide spectrum of academic publications, the paper provides an overview of regeneration and outdoor public lighting objectives. Emphasize is put on the obstacles for lighting...
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Peptide modification with thiomalonoamide moiety
PublikacjaAbstract: Meldrum acid derivatives under the thermal decomposition are well known source of ketenes. Recently we investigated chemical properties of thiocarbamolyl meldrum acids. We found that 5-(α-alkylamino- α'-sulfhydryl)methylene Meldrum's acids hated in the presence of amines produce thiomalonamides. We used this reaction to the one pot synthesis of series of short peptides containing thiomalonoamide motif.
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Excitation energy migration in uniaxially oriented polymer films.
PublikacjaPrzedstawiono wyniki pomiarów i symulacji Monte Carlo anizotropii emisji dla procesu bezpromienistego transportu energii w orientowanych osiowo foliach polimerowych. Badany układ stanowiły molekuły DTCI (3,3´-diethylthiacarbocyamine iodine) umieszczony w folii PVA (poliwinyl). Za przestrzenny rozkład orientacji dipoli emisji molekuł w symulacjach Monte Carlo przyjęto rozkład Tanizaki.
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Paweł Madej mgr inż.
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Stromal Derived Factor-1 (SDF-1) and Its Receptors CXCR4 and CXCR7 in Endometrial Cancer Patients
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Assessment of the SFlt-1 and sFlt-1/25(OH)D Ratio as a Diagnostic Tool in Gestational Hypertension (GH), Preeclampsia (PE), and Gestational Diabetes Mellitus (GDM)
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Serum 25(OH) Vitamin D Levels in Polish Women during Pregnancies Complicated by Hypertensive Disorders and Gestational Diabetes
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Ocena ekonomiczna recyklingu krzemowych ogniw fotowoltaicznych, realizowanego w skali laboratoryjnej
PublikacjaRecykling zużytych modułów PV umożliwia odzysk cennych i wartościowych materiałów wtórnych, które nadają się do ponownego zastosowania. Poruszane zagadnienia- ważne z punktu widzenia oszczędności energii i zmniejszania obciążenia środowiska- powinny również przyczynić się do lepszego projektowania, wykonawstwa oraz eksploatacji krzemowych modułów PV. Przeprowadzona wstępna analaiza ekonomiczna procesu recyklingu zużytych krzemowych...
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Photovoltaic Roof Tiles: The Influence of Heat Recovery on Overall Performance
PublikacjaA photovoltaic (PV) roof tile serves both as a roofing material and as an electricity-producing surface. The main aim of the present study was to increase the overall system efficiency of PV tiles by using heat recovery. The chosen strategy was two-fold: determine the operational efficiency of PV tiles and optimize construction to increase the cooling effect when using air as the working medium. A detailed experimental study was...
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Fast Low-fidelity Wing Aerodynamics Model for Surrogate-Based Shape Optimization
PublikacjaVariable-fidelity optimization (VFO) can be efficient in terms of the computational cost when compared with traditional approaches, such as gradient-based methods with adjoint sensitivity information. In variable-fidelity methods, the directoptimization of the expensive high-fidelity model is replaced by iterative re-optimization of a physics-based surrogate model, which is constructed from a corrected low-fidelity model. The success...
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Application of gas chromatography,mass spectrometry and olfactometry for quality assessment of selected food products = Zastosowanie chromatografii gazowej, spektrometrii mas i olfaktometrii w ocenie jakości wybranych produktów spożywczych
PublikacjaStosując mikroekstrakcję do fazy stacjonarnej z fazy nadpowierzchniowej jako metodę przygotowania próbek i chromatografię gazową (GC) ze spektrometrią mas (MS) i olfaktometrią (O) jako metodę oznaczeń końcowych oznaczono lotne związki w spirytusach i miodach. Identyfikację przeprowadzono przez porównanie widm masowych z widmami z biblioteki NIST. Dodatkowo, wykrywane przez panel oceniający związki zapachowe rejestrowano w formie...
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Connected & Automated Urban Mobility, Zombie Cars and Kitchen Knives: Will Autonomous Automobiles, Self-Driving Car-Sharing and Ride-Hailing, and Driverless Shuttles Harm Cities?
PublikacjaThere is a deficiency of supportive evidence to verify thesis that cities are prepared for mobility revolution which already begun and will advance exponentially. Furthermore, present revisions mostly emphasize how emerging mobility solutions will alter transport efficiency or affect mass transit usage, rather than on urban morphology, metropolitan society, nor local communities. Author is thoroughly concerned that municipalities...
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Labour share and income inequalities in the European Union, taking into account the level of development of economies
Publikacjaesearch background: The relationship between labour share and income inequality is a complex and multifaceted problem. Despite ongoing discussions among economists, there is still no consensus on the direction of the relationship between labour share and income ine-quality. Purpose of the article: The article aims to assess the impact of labour share on income inequal-ity, which is measured in three ways: the Gini index...
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Determinants of the incidence of non-academic staff in European and US HEIs
PublikacjaIn this article, we contribute to the scant literature covering quantitative studies on the determinants of the non-academic staff incidence in higher education institutions by analysing how the proportion of non-academic staff is related to key features such as size, prestige, year of foundation and financial structure of universities. We apply nonlinear regression analysis to compare HEIs across Europe and the USA, taking into...
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Post-Effort Changes in Autophagy- and Inflammation-Related Gene Expression in White Blood Cells of Healthy Young Men
PublikacjaAcute, strenuous physical exertion requiring high levels of energy production induces the production of reactive oxygen species and metabolic disturbances that can damage the mitochondria. Thus, selective autophagic elimination of defective mitochondria may improve resistance to oxidative stress and potentially to inflammation. The main goal of this study was to evaluate the impacts of intense effort on changes in the expression...
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CURRENT TRENDS IN RECYCLING OF PHOTOVOLTAIC SOLAR CELLS AND MODULES WASTE
PublikacjaIn comparison to other energy producing techniques, photovoltaics (PV) is one of the most promising options: no emission of any matter into the environment during operation; extremely long operation period (estimated average: 25 years), minimum maintenance, robust technique, aesthetic aspects. The use of photovoltaics is rapidly increasing, and the respective market is developing accordingly. Although PV manufacturing equipment...
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Recycling of Photovoltaic Solar Cells and Modules - Te State - Of - Art
PublikacjaIn comparison to other energy producing techniques, photovoltaics (PV) is one of the most promising options: no emission of any matter into the environment during operation; extremely long operation period (estimated average: 25 years), minimum maintenance, robust technique, aesthetic aspects. The use of photovoltaics is rapidly increasing, and the respective market is developing accordingly. Although PV manufacturing equipment...
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Energy Yield Generated by a Small Building Integrated Photovoltaic Installation
PublikacjaIn the recent years photovoltaic (PV) industry has experienced a major growth, caused by the ever present annual decrease in module production prices and the expanding awareness of the general public in terms of renewable energy. There are numerous ways to implement PV modules as an additional energy source for a building, be it mounted on the rooftop, or building integrated (BIPV). An analysis of BIPV consisting of 8 modules with...
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Evaluation of fire hazard in electrical installations due to unfavorable ambient thermal conditions
PublikacjaEvaluation of fire hazard caused by electrical installations is a difficult and important topic, and it limits the possibility of loss of life or health of people, as well as the occurrence of significant property damage. In particular, this hazard was caused by power cables and especially photovoltaic (PV) installations. The authors proved that power cables directly exposed to strong solar radiation are likely to exceed maximum...
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Lighting design for urban revitalization. The case of Poland
PublikacjaThis paper reflects on the basic research done in the area of public lighting in regard to revitalization processes. The general purpose of this scientific paper is to research a role of an implementation of public lighting design within revitalization frameworks. Referring to a wide spectrum of academic publications, the paper provides an overview of revitalization and public lighting objectives. The emphasize is put on a need...
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Aspekty ekologiczne i ekonomiczne recyklingu krzemowych ogniw i modułów fotowoltaicznych - efektywne wykorzystanie zasobów energetycznych.
PublikacjaStale rosnący udział modułów fotowoltaicznych (PV) w światowej produkcji energii elektrycznej powoduje, iż zwiększająca się ilość odpadów - w postaci zużytych lub uszkodzonych ogniw i modułów PV - spowoduje w najbliższych latach konieczność bardziej racjonalnego ich zagospodarowania. W artykule przedstawiono doświadczenia i wnioski autorów z realizacji badań, związanych z recyklingiem ogniw i modułów fotowoltaicznych z krystalicznego...
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Wpływ środowiska spawania na własności złączy próbnych Tekken ze stali S355J2C+N
PublikacjaIstnieją trzy metody spawania pod wodą, sucha, mokra oraz pośrednia między nimi, wykorzystująca lokalną komorę suchą. Ze względu na niskie koszty najszerzej rozpowszechnioną jest metoda mokra z wykorzystaniem elektrod otulonych. Woda jako środowisko spawania znacznie utrudnia przeprowadzenie procesu, którego realizacja niesie ze sobą wiele problemów. Pierwszym z nich jest zła widoczność po-wstającego złącza oraz niestabilność...