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Search results for: ESCHERICHIA COLI . WASTEWATER TREATMENT PLANT . MARINE OUTFALLS . INTEGRONS . GENE CASSETTES . ANTIBIOTIC RESISTANCE
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A Concept for Safe and Less Expensive Acceleration of a Marine Steam Turbine Start-up
PublicationThis paper analyses the issue of accelerated start-up of a marine steam turbine, which is an important problem because the start-up of a steam machine involves the combustion of fuel that is not transformed into useful energy. To find novel technologies that offer improvements in this aspect is essential due to restrictions on reducing ship emissions. Thus, the shorter the start-up time, the better for the environment and economy....
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Study of the influence of medium composition on motility and aggregation of recombinant Escherichia coli strain AAEC191A/pCC90 Dra D-mut-PBS, PBS+0.2% glucose, PBS+0.5% glucose
Open Research DataMicrobial motility is a fundamental aspect of many microbial life cycles and is a key survival mechanism that enables microorganisms to navigate diverse and dynamic environmental conditions. This phenomenon becomes particularly important in response to changes in stimuli in time and space. The following experiment aimed to investigate how the composition...
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Study of the influence of medium composition on motility and aggregation of recombinant Escherichia coli strain AAEC191A/pCC90 Dra D-mut-LB, LB+0.2% glucose, LB+0.5% glucose
Open Research DataMicrobial motility is a fundamental aspect of many microbial life cycles and is a key survival mechanism that enables microorganisms to navigate diverse and dynamic environmental conditions. This phenomenon becomes particularly important in response to changes in stimuli in time and space. The following experiment aimed to investigate how the composition...
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Study of the influence of medium composition on motility and aggregation of recombinant Escherichia coli strain BL21(DE3)/pCC90 D54-STOP-LB, LB+0.2% glucose, LB+0.5% glucose
Open Research DataMicrobial motility is a fundamental aspect of many microbial life cycles and is a key survival mechanism that enables microorganisms to navigate diverse and dynamic environmental conditions. This phenomenon becomes particularly important in response to changes in stimuli in time and space. The following experiment aimed to investigate how the composition...
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Study of the influence of medium composition on motility and aggregation of recombinant Escherichia coli strain BL21(DE3)/pCC90 D54-STOP-PBS, PBS+0.2% glucose, PBS+0.5% glucose
Open Research DataMicrobial motility is a fundamental aspect of many microbial life cycles and is a key survival mechanism that enables microorganisms to navigate diverse and dynamic environmental conditions. This phenomenon becomes particularly important in response to changes in stimuli in time and space. The following experiment aimed to investigate how the composition...
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Application of Eco-innovative Technologies of Nutrients Removal in Wastewater – Case Study BARITECH Project
PublicationEco-innovative technologies in wastewater treatment should provide not only stringent standards for the quality of treated wastewater but also ensure maximum recovery of energy and raw materials from wastewater. One of the ways to improve the removal efficiency of nitrogen and phosphorus compounds in existing conventional wastewater treatment plants is pretreatment of reject water generated during the mechanical...
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Web-based GIS as a tool for supporting marine research
PublicationZbieranie danych morskich podczas badań hydroakustycznych jest złożonym zadaniem, na które składają się nie tylko pomiary, ale także analiza, weryfikacja i interpretacja informacji uzyskanych przez różne czujniki. Automatyczna integracja, wizualizacja i przetwarzanie zgromadzonych danych pozwala na bardziej precyzyjne rozpoznanie badanych zjawisk. W pracy przedstawiono dedykowany sieciowy system syntezy i rozpowszechniania danych...
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Constituents of organic alkalinity in pore water of marine sediments
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Hopf bifurcation in time‐delayed gene expression model with dimers
PublicationWe study a mathematical model of gene transcription and protein synthesis with negative feedback. We consider a system of equations taking into account the formation of dimers (i.e., complex formed by two protein monomers), the way in which dimers bind to DNA and time delay in translation process. For the model consisting of three ordinary differential equations with time delay, we derive conditions for stability of the positive...
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Hydrochar-nanoparticle integration for arsenic removal from wastewater: Challenges, possible solutions, and future horizon
PublicationArsenic (As) contamination poses a significant threat to human health, ecosystems, and agriculture, with levels ranging from 12 to 75% attributed to mine waste and stream sediments. This naturally element is abundant in Earth's crust and gets released into the environment through mining and rock processing, causing ≈363 million people to depend on As-contaminated groundwater. To combat this issue, introducing a sustainable hydrochar...
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INFLUENCE OF LOAD AND INFLATION PRESSURE ON THE TYRE ROLLING RESISTANCE
PublicationTyre load and inflation pressure are important factors controlling rolling resistance of road vehicles. The article presents results obtained in the Technical University of Gdańsk during laboratory and road measurements of different car tyres rolling on different pavements. The knowledge of rolling resistance characteristics is important for modelling car dynamics as well as fuel consumption. It is also necessary to establish proper...
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INFLUENCE OF TEMPERATURE ON THE TYRE ROLLING RESISTANCE
PublicationTemperature is a very important factor controlling rolling resistance of road vehicle tyres. There are at least three different temperatures that may be considered as important factors controlling thermal conditions of the rolling tyre. The most common measure of the thermal conditions during tyre rolling is ambient air temperature. The other two are: pavement temperature and “tyre” temperature. Tyre temperature is the most difficult...
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Engineering method of tire rolling resistance evaluation
PublicationTire rolling resistance is one of the most difficult tire parameters to measure. The reason is that for modern tires the force of rolling resistance corresponds to 0.5–1% of tire load, thus measurements of very small forces must be performed in a heavily loaded system. This constitutes great problems, especially in road conditions. Laboratory measurements are easier to perform, as the environment may be better controlled, but roadwheel...
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Sonocatalytic degradation of tetracycline antibiotic using zinc oxide nanostructures loaded on nano-cellulose from waste straw as nanosonocatalyst
PublicationThe aim of the present investigation was the combination of ZnO nanostructures with nano-cellulose (NC) for the efficient degradation of tetracycline (TC) antibiotic under ultrasonic irradiation. The removal efficiency of 12.8% was obtained by the sole use of ultrasound (US), while the removal efficiency increased up to 70% by the US/ZnO treatment process. Due to the integration of ZnO nanostructures with NC, the removal efficiency...
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Advantages of continuous Fenton reaction over the traditional batch process in wastewater treatment in a single and two-step mode
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Low frequency of itraconazole resistance found among avian Aspergillus fumigatus isolates
PublicationAspergillus fumigatus is the most frequent etiological factor of invasive aspergillosis in human as well as animals, including birds. Aspergillosis caused by drug-resistant strains poses a significant therapeutic issue, especially if resistance pattern includes insensitivity to triazoles, i.e. drugs recommended for the primary therapy purposes. Majority of resistant isolates hold the TR34/L98H...
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Corrosion and Cavitation Resistances of Low Alloy Steel After Frictional-Mechanical Treatment
PublicationPaper presents the results of the studies of wear, corrosion and cavitation resistances of the S355J2 low alloy medium carbon steel subjected to surface modification by friction-mechanical treatment, as tested in sea water simulated solution. To evaluate the effect of the surface treatment, the comparative studies of the corrosive properties of the steel before and after treatment have been considered. The treatment due to a severe...
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Road texture influence on tire rolling resistance
PublicationTire rolling resistance is one of the most important resistive forces acting on road vehicles. It depends on tire and surface characteristics as well as vehicle operating parameters. This paper deals with the influence of road surface texture on rolling resistance of passenger car tires. Results of road measurements performed on variety of road surfaces are presented.
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Regeneration of marine engine valves using laser surfacing
PublicationThis article presents an analysis of the applicability of laser surfacing of the exhaust valve face of a marine diesel engine using cobalt-based powder. After preparation by machining, the selected valves were subjected to laser surfacing using a high-power ROFIN DL020 laser. The EuTroLoy 16012 powder was used in the surfacing process. One of the valves was cut and subjected to metallographic examination and hardness measurements,...
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Tyre rolling resistance and its influence on fuel consumption
PublicationRolling resistance of tyres is one of the major resistive forces acting on any wheeled vehicle. Unfortunately, it is also one of the forces very difficult to measure. It is estimated that in certain traffic conditions (like for example constant speed driving with slow or moderate speed) so called Rolling Resistance Impact Factor may be as high as 0.3. This means that reduction of rolling resistance by 50% would lead to 15% of energy...
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Transport deficiency is the molecular basis of Candida albicans resistance to antifungal oligopeptides
Publication(FMDP), an inhibitor of glucosamine-6-phosphate synthase, exhibited growth inhibitory activity against Candida albicans, with minimal inhibitory concentration values in the 0.05–50 mg/L range. Uptake by the peptide permeases was found to be the main factor limiting an anticandidal activity of these compounds. Di- and tripeptide containing FMDP (F2 and F3) were transported by Ptr2p/Ptr22p peptide transporters (PTR) and FMDP-containing...
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Antibacterial Activity and Cytocompatibility of Bone Cement Enriched with Antibiotic, Nanosilver, and Nanocopper for Bone Regeneration
PublicationBacterial infections due to bone replacement surgeries require modifications of bone cement with antibacterial components. This study aimed to investigate whether the incorporation of gentamicin or nanometals into bone cement may reduce and to what extent bacterial growth without the loss of overall cytocompatibility and adverse effects in vitro. The bone cement Cemex was used as the base material, modified either with gentamicin...
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The thermal effort during marine steam turbine flooding with water
PublicationThe work discussed an extreme case of cooling a steam turbine. The ship's steam turbine was used as an example. In marine transport units with low-speed engines dominate, however, there are also units with steam turbines. An example of analysed marine steam turbine is shown in Fig. 1. When considering the issue of cooling steam turbines, it is necessary to estimate the extreme conditions that may occur during operation of the turbine....
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Study of the influence of medium composition on motility and aggregation of recombinant Escherichia coli strain BL21(DE3)/pCC90 Dra D-mut-PBS, PBS+0.2% glucose, PBS+0.5% glucose
Open Research DataMicrobial motility is a fundamental aspect of many microbial life cycles and is a key survival mechanism that enables microorganisms to navigate diverse and dynamic environmental conditions. This phenomenon becomes particularly important in response to changes in stimuli in time and space. The following experiment aimed to investigate how the composition...
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Study of the influence of medium composition on motility and aggregation of recombinant Escherichia coli strain BL21(DE3)/pCC90 Dra D-mut-LB, LB+0.2% glucose, LB+0.5% glucose
Open Research DataMicrobial motility is a fundamental aspect of many microbial life cycles and is a key survival mechanism that enables microorganisms to navigate diverse and dynamic environmental conditions. This phenomenon becomes particularly important in response to changes in stimuli in time and space. The following experiment aimed to investigate how the composition...
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INFLUENCE OF DREDGER POWER PLANT TYPE AND DREDGER SIZE ON OUTPUT OF POWER PLANT INSTALLED ON TRAILING SUCTION HOPPER DREDGER
PublicationThis paper presents results of investigations dealing with influence of parameters characterizing size of suction hopper dredger as well as type of power system on magnitude of total output of combustion engines installed in dredger power plant.
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Decreasing CO2 Emissions By Reducing Tire Rolling Resistance
PublicationThe first motor vehicle was built by Nicolas Cugnot almost 250 years ago and since then there has been a continuous process of motor vehicles’ improvements, as well as development of new road pavements. The fuel crisis of the 70s of the 20th century made it clear to vehicle builders that measures should be taken to reduce vehicle fuel consumption and it contributed to an increased interest in electric vehicles. Although significant...
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Pathways of Nitrogen Removal in Hybrid Treatment Wetlands
PublicationHybrid Treatment Wetlands (HTWs) are composed of two or more filters with different modes of flow, allowing the benefits of both types of bed to be combined, resulting in better effluent quality (nitrogen and organic compounds removal). Such a heterogeneous environment creates possibilities for different mechanisms of nitrogen removal. The objective of the present study was to investigate the removal of nitrogen versus a range...
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Influence of Road Wetness on Tire-Pavement Rolling Resistance
PublicationRolling resistance of tires is one of the most important factors influencing energy consumption of road vehicles, especially on rural roads. For practical reasons, most of rolling resistance measurements are usually performed for dry road conditions. Based on the fact that roads are wet during a considerable time over the year and as part of the projects MIRIAM, ROLRES and ROSANNE, the TUG (Technical University of Gdańsk) in Poland...
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The importance and availability of marine certified reference materials
PublicationTraceability and reliable results are the two pillars of analytical methods; certified reference materials (CRMs) meet this requirement. ISO 17034:2016 credentials provide brief information on general requirements for the competence of Reference Material Producers (RMPs). The different types of CRMs have been produced in recent years for chemical analysis in food, water, soil, and sediment matrices in recent years. This review...
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The sensitivity of bacteria to heavy metals in presence of mineral ship motor oil in coastal marine sediments and waters.
PublicationThe sensitivity of bacteria, isolated from coastal marine sediments and waters of Sopot beach, Gdańsk Bay (Poland), to heavy metals in the presence of mineral ship motor oil were studied. All isolated heterotrophic bacteria were resistant to the 0.1 mM lead concentration occurring in the environment. Metal resistance of bacteria isolated from coastal water was strongly related to mineral ship motor oil concentration. At 0.1% mineral...
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Analysis of the macrostructure of the fuel spray atomized with marine engine injector
PublicationOne of the main problem influencing the combustion process in the cylinder of the marine engine is an fuel spray phenomena. The parameters describing the shape of the fuel spray are named macro parameters. This article presents the research results of the macrostructure parameters of the fuel spray atomized with the marine engine injector. The research were carried out by optical visualization measurement method of Mie scattering....
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DNA structure and integrity checkpoints in the cell cycle and their role in drug targeting and resistance of tumor cells to anticancer treatment
PublicationW artykule dokonaliśmy przeglądu literatury dotyczącej różnych typów uszkodzeń DNA indukowanych przez różne czynniki terapeutyczne w komórkach nowotworowych. Omówiliśmy także w jaki sposób aktywują różne typy punktów kontrolnych w cyklu komórkowym i w jaki sposób punkty te są regulowane przez inne szlaki sygnalizacji wewnątrzkomórkowej takie jak szlaki naprawy uszkodzeń DNA, stresu komórkowego i śmierci-przeżycia. W szczególności...
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Non-Nutritive Bioactive Compounds in Food of Plant Origin
PublicationThe increasing knowledge on the health benefits of certain food ingredients, in particular, those of plant origin, opened the discussion of the possibility of using edible plants or their active components in the prevention of non-communicable diseases. The health-promoting properties of plant foods are related to the presence of non-nutritive compounds, mainly plant secondary metabolites, which can affect many biological mechanisms...
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Research on the effect of low-sulphur marine fuels on the dynamic characteristics of a CI engine
PublicationThe implementation of low-sulphur, so-called modified marine fuels into operation requires prior labora- tory engine tests to assess the energy, emission and structural effects of their usage. This type of research are carried out on the test bed of a diesel engine as a small-scale physical model that reproduces the adequate design and process (parametric) features of a full-size marine engine. Their key stage is to determine the energy...
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Advancing sustainable wastewater management: A comprehensive review of nutrient recovery products and their applications
PublicationWastewater serves as a vital resource for sustainable fertilizer production, particularly in the recovery of nitrogen (N) and phosphorus (P). This comprehensive study explores the recovery chain, from technology to final product reuse. Biomass growth is the most cost-effective method, valorizing up to 95 % of nutrients, although facing safety concerns. Various techniques enable the recovery of 100 % P and up to 99 % N, but challenges...
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Mathematical analysis of a generalised p53-Mdm2 protein gene expression model
PublicationWe propose the generalisation of the p53-Mdm2 protein gene expression model introduced by Monk (2003). We investigate the stability of a unique positive steady state and formulate conditions which guarantee the occurrence of the Hopf bifurcation. We show that oscillatory behaviour can be caused not only by time lag in protein transcription process, but also can be present in the model without time delay. Moreover, we investigate...
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Expression of cyclin D1 after treatment with doxorubicin in the HL-60 cell line
PublicationIncreased levels of cyclin D1 and amplification of CCND1 gene occur in many types of cancers. We have followed the expression of cyclin D1 after treatment with doxorubicin with reference to cell death and other possible therapeutic implications. The effect of the treatment on the cell cycle, survival, intracellular level (flow cytometry), and intracellular localization of cyclin D1 (fluorescence microscopy) and expression of...
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Security Assessment of a Turbo-Gas Power Plant
PublicationCritical infrastructures are exposed to new threats due to the large number of vulnerabilities and architectural weaknesses introduced by the extensive use of information and communication technologies. This paper presents the results of an exhaustive security assessment for a turbo-gas power plant.
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Absolute configurations of C34 and C35 of antibiotic niphimycin A
PublicationThe relative configurations of four stereogenic centers of the C33-C42 fragment of niphimycin A were assigned as 2S*, 3R*, 4S* and 6S*, based upon 1H NMR analysis with double-quantum filtered COSY and nuclear Overhauser spectroscopy experiments. These data were then correlated with absolute configurations at C36 and C38 of niphimycin A, which were declared previously as 36S and 38S [3]. This allowed for the assignment of the absolute...
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Starter cultures as a reservoir of antibiotic resistant microorganisms
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Absolute configurations of C34 and C35 of antibiotic niphimycin A
PublicationThe relative configurations of four stereogenic centers of the C33-C42 fragment of niphimycin A were assigned as 2S*, 3R*, 4S* and 6S*, based upon (1)H NMR analysis with double-quantum filtered COSY and nuclear Overhauser spectroscopy experiments. These data were then correlated with absolute configurations at C36 and C38 of niphimycin A, which were declared previously as 36S and 38S [3]. This allowed for the assignment of the...
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Mechanism of Binding of Antifungal Antibiotic Amphotericin B to Lipid Membranes: An Insight from Combined Single-Membrane Imaging, Microspectroscopy, and Molecular Dynamics
PublicationAmphotericin B is a lifesaving polyene antibiotic used in the treatment of systemic mycoses. Unfortunately, the pharmacological applicability of this drug is limited because of its severe toxic side effects. At the same time, the lack of a well-defined mechanism of selectivity hampers the efforts to rationally design safer derivatives. As the drug primarily targets the biomembranes of both fungi and humans, new insights into the...
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TEMPERATURE INFLUENCE ON TIRE ROLLING RESISTANCE MEASUREMENTS QUALITY
PublicationGlobal warming makes it necessary to reduce energy consumption, which in the case of motor vehicles, is connected, among other things, with reduction of resistive forces acting on a vehicle during its motion. One of the most important components of those forces is rolling resistance, which is very difficult to measure, especially in road conditions. The article deals with issues related to the influence of the thermal state of...
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Multibeam Sonar Characterisation of Seafloor in the Context of Visualisation and Dissemination of Marine Data
PublicationThe paper presents the seafloor characterisation method based on multibeam sonar data. it relies on using three types of multibeam seafloor sensing data: 1) the grey-level sonar images (echograms) of seabed, 2) the 3D model of the seabed surface which consists of high resolution bathymetric data, 3) the set of time domain bottom echo envelopes received in the consecutive sonar beams. The classification is performed by utilisation...
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WATER LUBRICATED MAIN SHAFT BEAR INGS WITH THREE LAYER BUSH – MODERN SOLUTION FOR MARINE INDUSTRY
PublicationWater lubricated sliding bearings are increasingly popular in marine and hydro power industry. Such popularity is partly due to their simple construction which also means a relatively affordable price. Properly designed and installed water lubricated bearings may well last for over a decade. During the last decade their traditional range has been expanded with new, modern products like three layer bearing bush. The work presents...
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Analysis of Rolling Resistance of Tires With Run Flat Insert
PublicationRun flat properties are required for tires intended for special applications like military or rescue vehicles. Run-Flat inserts make possible to drive a car or truck, with certain restrictions, also when inflation pressure is very low or non-existing (after tire damage). Rolling resistance of tires rolling the inserts increases considerably in relation to the rolling resistance of properly inflated tires. This paper presents results...
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Intramolecular transformation of an antifungal antibiotic nystatin A1 into its isomer, iso-nystatin A1 - structural and molecular modeling studies
PublicationNystatin A1, a polyene macrolide antifungal antibiotic, in a slightly basic or acidic solution undergoes an intramolecular transformation, yielding a structural isomer, the translactonization product, iso-nystatin A1 with lactone ring diminished by two carbon atoms. Structural evidence is provided by advanced NMR and Mass Spectrometry (MS) studies. Molecular dynamics simulations and quantum mechanics calculations gave the insight...
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The Relationship between Gene Activity and Nitrous Oxide Production during Nitrification in Activated Sludge Systems
PublicationNitrite is an important factor which inhibits the first stage of the nitrification, the effect is stronger with the decrease of the DO concentrations. NO2- presence at concentration above 15 mg N – NO2 in the aeration tanks stimulates N2O production regardless of the DO concentration. The significant nirS gene induction, observed especially during the experiments with the nitrite addition indicates that N2O production is basically...
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Modernization of the Trailer for Tyre/Road Rolling Resistance Measurements
PublicationIn the article the ways of defining tyre rolling resistance are presented. The advantages of the laboratory tests of tyre/road rolling resistance and the advantages and disadvantages of on the road tyre/road rolling resistance tests are described. The description of the special trailer used for tyre/road rolling resistance measurements designed and constructed in the Faculty of Mechanical Engineering at Gdansk University of Technology,...