Search results for: LAPC (YEJM)
-
Regulation of the First Committed Step in Lipopolysaccharide Biosynthesis Catalyzed by LpxC Requires the Essential Protein LapC (YejM) and HslVU Protease
PublicationWe previously showed that lipopolysaccharide (LPS) assembly requires the essential LapB protein to regulate FtsH-mediated proteolysis of LpxC protein that catalyzes the first committed step in the LPS synthesis. To further understand the essential function of LapB and its role in LpxC turnover, multicopy suppressors of ΔlapB revealed that overproduction of HslV protease subunit prevents its lethality by proteolytic degradation...
-
A new factor LapD is required for the regulation of LpxC amounts and lipopolysaccharide trafficking
PublicationLipopolysaccharide (LPS) constitutes the major component of the outer membrane and is essential for bacteria, such as Escherichia coli. Recent work has revealed the essential roles of LapB and LapC proteins in regulating LPS amounts; although, if any additional partners are involved is unknown. Examination of proteins co-purifying with LapB identified LapD as a new partner. The purification of LapD reveals that it forms a complex...
-
LDPC Codes performance investigation
Publication...
-
Badanie efektywności kodów LDPC
PublicationW referacie zaprezentowano wyniki badań symulacyjnych jakości transmisji z wykorzystaniem kodów LDPC i turbokodu w interfejsie radiowym systemu UMTS Badania symulacyjne zostały przeprowadzone dla szybkości transmisji 144 kb/s w środowisku propagacyjnym miejskim, wokołobudynkowym. Oprócz tego została przedstawiona zasada działania i algorytmy dekodowania kodów LDPC.
-
Porównanie efektywności kodów LDPC
PublicationW referacie zaprezentowano porównanie wyników badań symulacyjnych jakości transmisji z wykorzystaniem kodów LDPC w interfejsie radiowym systemu UMTS. Badania symulacyjne zostały przeprowadzone dla szybkości transmisji 144 kb/s w środowisku propagacyjnym Outdoor to Indoor & Pedestrian B. W referacie przedstawiono również zasadę działania i algorytmy dekodowania kodów LDPC.
-
Suppressors of lapC mutation identify new regulators of LpxC, which mediates the first committed step in lipopolysaccharide biosynthesis
PublicationGram-negative bacteria, such as Escherichia coli, are characterized by an asymmetric outer membrane (OM) with lipopolysaccharide (LPS) located in the outer leaflet and phospholipids facing the inner leaflet. E. coli recruits LPS assembly proteins LapB, LapC and LapD in concert with FtsH protease to ensure a balanced biosynthesis of LPS and phospholipids. We recently reported that bacteria either lacking the periplasmic domain of...
-
Regulation of LPS assembly via controlled proteolysis and sensing of LPS stress in Escherichia coli
PublicationLipopolysaccharide (LPS) is a complex glycolipid, essential for the bacterial viability and along with phospholipids, it constitutes the major amphiphilic component of outer membrane (OM) in most of the Gram-negative bacteria, including Escherichia coli. LPS molecules confer an effective permeability barrier to the OM and play a crucial role in bacteria-environment and -host interactions. The synthesis and accumulation of this...
-
BADANIE EFEKTYWNOŚCI DEKODOWANIA ITERACYJNEGO KODÓW LDPC
PublicationW referacie zaprezentowano wyniki badań symulacyjnych jakości transmisji z wykorzystaniem nieregularnych kodów LDPC. Badania przeprowadzono z wykorzystaniem modulacji BPSK w kanale z addytywnym szumem gaussowskim AWGN. Na wykresach zostały przedstawione zależności bitowej stopy błędów BER od liczby iteracji dekodera oraz zależność liczby iteracji, które trzeba wykonać, aby uzyskać korekcję wszystkich błędów dla różnych wartości...
-
Monitoring of single-side lap grinding with electroplated tools
PublicationThe results presented in the paper show that utilising microphones as audible sound sensors is a suitable approach for monitoring a single-side lap grinding process due to the low levels of noise generated by the drives of a machine tool. The results confirm that sound signal analysis is a feasible and relatively simple method to monitor a lap grinding process with the use of an audible sound sensor. Proposed method can be used...
-
Lamb wave propagation in a single lap adhesive joint
Publication -
Influence on micrograins size on the distance between the workpiece and the lap.
PublicationNa kształtowanie powierzchni docieranej główny wpływ ma błąd kształtu zastosowanego narzędzia. Intensywność docierania zależy od wielkości zastosowanych ziaren ściernych, które również wpływają na błędy kształtu przedmiotu. W referacie przedstawiono wyniki obliczeń odległości przedmiotu od powierzchni narzędzia w funkcji wielkości ziaren ściernych.
-
Lamb wave propagation in a single lap adhesive joint
PublicationThe aim of the paper is the analysis of Lamb wave propagation in adhesive joints. The research was performed on a single lap adhesive joint of two steel plates. Two types of joints, namely an intact and with defect were considered. In experimental investigations the condition assessment of the joint was performed with the use of antisymmetric Lamb waves excited by a plate piezo actuators and measured by a laser vibrometer. Numeri-cal...
-
Assembly of Lipopolysaccharide in Escherichia coli Requires the Essential LapB Heat Shock Protein
PublicationHere, we describe two new heat shock proteins involved in the assembly of LPS in Escherichia coli, LapA and LapB (lipopolysaccharide assembly protein A and B). lapB mutants were identified based on an increased envelope stress response. Envelope stress responsive pathways control key steps in LPS biogenesis and respond to defects in the LPS assembly. Accordingly, the LPS content in ΔlapB or Δ(lapA lapB) mutants was elevated, with...
-
Diagnostics of bolted lap joint using guided wave propagation
PublicationThe paper presents numerical and experimental analyses of elastic waves propagation in a bolted lap joint. In experimental investigations condition assessment of the joint was performed with the use of symmetric waves excited by a piezoactuator. Numerical calculations were conducted in commercial finite element method software Abaqus. The influence of number of bolts and the value of the initial stress on recorded signals was examined....
-
Damage detection in a bolted lap joint using guided waves
PublicationThe paper presents the experimental investigation of guided waves application to the condition assessment of prestressed bolted connections and a concept of new quantitative indicator. The main goal of the research was the analysis of the influence of the contact conditions changes to the characteristics of a propagating disturbance. The experimental tests were carried out for a single bolted lap joint. The excitation and acquisition...
-
Lamb wave-based monitoring of shear failure of an adhesive lap joint
PublicationThe paper presents a study on the elastic wave propagation in adhesive joints of steel plates subjected to tensile loading. A single lap joint was chosen for analysis because of its simplicity and plurality of applications. Experimental investigations consisted of the uniaxial extension of prepared specimens. Force and displacement values were recorded by a testing machine. Simultaneously, guided Lamb waves were excited and signals...
-
Wear of electroplated diamond tools in lap-grinding of Al2O3 ceramic materials
PublicationCurrent development of modern products, together with ever-increasing demands for their operation and usage, necessitate the search for new processing methods. Abrasive machining is widely used in many industrial areas, especially for processing difficult-to-machine materials such as advanced ceramics. Grinding with lapping kinematics, also called lap-grinding, is still one of the innovative methods of abrasive processing being...
-
New kinematic systems in single-sided lapping and their influence on lap wear
PublicationPrevious studies on one-sided lapping allow to state that not only parameters of lapping elements (i.e. properties of workpiece, abrasive grains and lapping plate) impact on a lapping efficiency. Influential are also kinematics and dynamics of the process. It is crucial to control an average velocity of lapping (v), a distribution of tangential acceleration (at), a nominal pressure (p), a lapping time (t) and disposal of workpiece...
-
Identyfikacja dźwięków serca za pomocą algorytmu LPC oraz sztucznej sieci neuronowej.
PublicationW artykule przedstawiono algorytm klasyfikacji sygnału fonokardiograficznego, który umożliwia skuteczną identyfikację 12 różnych stanów. Poprzez połączenie ze sobą algorytmu kodowania liniowego (LPC) wraz ze sztuczną siecią neuronową uzyskano skuteczność klasyfikacji sięgającą 82% oraz pełną skuteczność w rozróżnieniu pomiędzy stanami: braku lub występowania schorzenia. Najlepsze rezultaty uzyskano dla jednokierunkowych, dwuwarstwowych...
-
Wykorzystanie algorytmu kompresji sygnału mowy MP-LPC do modelowania przebiegu fonokardiograficznego
PublicationW artykule przedstawiono nową metodę modelowania dźwięków serca, która może znaleźć zastosowanie w systemach diagnostyki urządzeń medycznych oraz stanowić podstawę opracowania inteligentnego stetoskopu. Przedstawiona propozycja stanowi rozwinięcie algorytmu MP-LPC wykorzystywanego w kompresji sygnału mowy. Wykazano, że do dokładnego modelowania przebiegu fonokardiograficznego, który pozwala na odwzorowywanie różnorodnych stanów...
-
The use of Preston equation to determine material removal during lap-grinding with electroplated CBN tools
PublicationGrinding executed in a lapping configuration is an alternative finishing process benefiting from both grinding and free-abrasive machining, while minimizing the heat effect impact. Electroplated tools can be effectively used in different abrasive processes, including high-speed grinding, however, the assessment of machining performance over time is a key factor in their correct use to achieve satisfactory technological results....
-
Efficient FPGA Implementation of an RFIR Filter Using the APC–OMS Technique with WTM for High-Throughput Signal Processing
PublicationNowadays, Finite Impulse Response (FIR) filters are used to change the attributes of a signal in the time or frequency domain. Among FIR filters, a reconfigurable filter has the advantage of changing the coefficient in real-time, while performing the operation. In this paper, the Anti-Symmetric Product Coding (APC) and Odd Multiple Storage (OMS) modules are utilized to implement the reconfigurable FIR filter (RFIR–APC–OMS). Herein,...
-
The photodynamic effect of far-red range phthalocyanines (AlPc and Pc green) supported by electropermeabilization in human gastric adenocarcinoma cells of sensitive and resistant type
Publication -
Agnieszka Potęga dr hab. inż.
People -
NCN: Webinarium OPUS 24 + LAP/ Weave
EventsPodczas wydarzenia zostaną przybliżone założenia programu Weave oraz szczegółowo omówione zasady składania wniosków. Szkolenie poprowadzą pracownicy NCN.
-
Checkpoints that regulate balanced biosynthesis of lipopolysaccharide and its essentiality in Escherichia coli
PublicationThe outer membrane (OM) of Gram-negative bacteria, such as Escherichia coli, is essential for their viability. Lipopolysaccharide (LPS) constitutes the major component of OM, providing the permeability barrier, and a tight balance exists between LPS and phospholipids amounts as both of these essential components use a common metabolic precursor. Hence, checkpoints are in place, right from the regulation of the first committed step...
-
The Sejm Review
Journals -
LAP LAMBERT Academic Publishing
Publishing Houses -
Investigating the coordinated regulation of lipopolysaccharide (LPS) and phospholipid amounts by the essential LPS assembly proteins LapB/LapC and the involvement of a new thioesterase
ProjectsProject realized in Laboratorium Genetyki Bakterii according to UMO-2023/49/B/NZ1/01986 agreement
-
Polityczne, prawne i ekonomiczne aspekty ustawy o języku polskim
PublicationW artykule omówiono proces przyjmowania przez Sejm i Senat RP ustawy o języku polskim z 7 października 1999 r. oraz jej nowelizacji z 4 marca 2004 r. Przestawiono aspekty polityczne, prawne i ekonomiczne tej ustawy oraz wybrane komentarze prasowe.
-
System monitorowania prac organów przedstawicielskich - doświadczenie z zakresu e-demokracji
PublicationArtykuł zawiera wprowadzenie w tematykę e-demokracji oraz przedstawia uruchomiony w połowie roku 2004 ''System Monitorowania Prac Sejmu'', który udostępnia obywatelom informację dotyczącą zachowań ich przedstawicieli - posłów na Sejm RP. Przedstawiono obecną funkcjonalność systemu, zastosowane rozwiązania techniczne oraz plany rozwojowe.
-
The idea of a robotic single-sided lapping system
PublicationThis paper reviews the idea of a robotic lapping system. Robot’s assistance automates the lap-ping process and supports the development of a flexible lapping system. However, the main aim behind the idea of a robotic lapping system is to provide improved means for controlling the posi-tion of conditioning rings on a lapping plate. Due to the kinematics of lapping process, the profile wear of the tool is not constant along the radius....
-
Rok 2011 rokiem Jana Heweliusza
PublicationSejm Rzeczypospolitej Polskiej wraz z Radą Miasta Gdańska ustanowił rok 2011 ''Rokiem Jana Heweliusza''. poprzez organizację szeregu naukowych i kulturalnych wydarzeń skierowanych do różnych grup społecznych Miasto Gdańsk świętuje czterechsetną rocznice urodzin Wielkiego Gdańszczanina, którego odkrycia przyczyniły się do rozwoju europejskiej nauki.
-
Poszukiwanie sankcji optymalnej w perspektywie projektu ustawy z dnia 7 lipca 2022 r. o zmianie ustawy Kodeks karny oraz niektórych innych ustaw. The search for the optimal sanction in the perspective of the draft act of 7 July 2022 amending the Penal Code and some other acts.
PublicationPolski ustawodawca opublikował w połowie września 2021 r. na stronie Rządowego Centrum Legislacji projekt gruntownej zmiany – między innymi – Kodeksu karnego. W dniu 7 lipca 2022 r. Sejm RP przyjął ww. projekt ustawy (druk sejmowy nr 2024). W zasadniczym stopniu nowelizacja - między innymi - zwiększa wysokość kar grożących za poszczególne przestępstwa. Ustawodawca – poprzez zabiegi legislacyjne –...
-
Shaping of the workpiece surface in single-disc lapping
PublicationShape errors of the tool exert a dominant influence on the shape accuracy of the workpiece. The correlation between flatness errors of the lap and flatness errors of the workpieces was checked experimentally and determined analytically. Computer model of the workpiece shaping by lapping was developed. Evaluation method of the workpiece orientation as well as some simulation results for a lap with shape errors of convexity and cincavity...
-
A Population-Based Method with Selection of a Search Operator
PublicationThis paper presents a method based on a population in which the parameters of individuals can be processed by operators from various population-based algorithms. The mechanism of selecting operators is based on the introduction of an additional binary parameters vector located in each individual, on the basis of which it is decided which operators are to be used to modify individuals’ parameters. Thus, in the proposed approach,...
-
Electroabsorption in triphenylamine-based hole-transporting materials for organic light-emitting diodes
PublicationMetodą elektroabsorpcji (EA) badano stany wzbudzenia elektronowego w próżniowo naparowanych warstwach diamin (TPD, TAPC) i dendrymeru aminowego m-MTDATA. Widma EA amin złożonych porównano z widmem monomeru trifenylaminy (TPA). Kształt widm EA świadczy o obecności zdegenerowanych i quasi-zdegenerowanych stanów elektronowych.
-
Technological Problems in Lapping on Flat Surfaces of Ceramic Parts
PublicationThis paper will present the results of research on single-disc lapping of flat surfaces of small ceramic elements. Machining with the use of different tools such as metallic, two-metallic and abrasive-metallic will be analyzed. The basics and techniques of reinforcement of laps will be discussed. The results of the influence of parameters on the surface quality of ceramic conponents (sealing) for three treatments on lapping machines...
-
Internal electric fields in vacuum-evaporated films as studied by electroabsorption spectroscopy
PublicationMierzono sygnały elektroabsorpcji na I-szej harmonicznej częstości przykładanego sinusoidalnego pola elektrycznego w komórkach sandwiczowych Al/warstwa organiczna/Al. Wyznaczono elektryczne pola wewnętrzne (Fi) dla materiałów stosowanych w organicznych diodach elektroluminescencyjnych (TPD, TAPC, MTDATA, QAC, Alq3, Ir(ppy)3, PtOEP). Mechanizm powstawania pól Fi powiązano z pułapkowaniem ładunku w obszarach przyelektrodowych.
-
Electronic structure of CeBa2Cu3O7.
PublicationPrzeprowadzono obliczenia ab initio struktury elektronowej związku CeBa2Cu3-O7(Ce123) metodą LAPW oraz porównano z analogicznymi strukturami otrzymanymi dla YBa2Cu3O7 (Y123), NdBa2Cu3O7(Nd123) i PrBa2Cu3O7(Pr123). Wszystkie wyliczone struktury pasmowe są zasadniczo podobne. W pracy postawiono hipotezę dotyczącą korelacji pomiędzy silnym rozszczepieniem spinowym w Pr123 i Ce123a niestabilnością strukturalną tych układów.
-
Migration of trace elements and radioisotopes to various fractions of solid wastes generated as a result of the sewage sludge incineration process
PublicationThe research was aimed at providing new knowledge in the field of chemical characteristics of solid waste generated in the process of combustion of sewage sludge in fluidized bed furnaces. The research material consisted of disposed fluidized beds (DFB), sewage sludge ash (SSA) and air pollution control residues (APC) from three Polish installations for the thermal treatment of sewage sludge. Natural radionuclides as well as anthropogenic...
-
Recurrent bowel-blood translocations of Escherichia coli with the unique virulence characteristics over three-year period in the patient with acute myeloid leukaemia – case report
PublicationIn patients with haematological malignancies, the bowel remains the main source of Escherichia coli bloodstream infections. We present the clinical example of recurrent bowel-blood translocations of E. coli with the unique virulence characteristics in a 55-year-old male with the diagnosis of acute myeloid leukaemia. The virulent factors profile of examined strains confirmed that the co-existence of genes papC, sfa, usp and cnf1,...
-
Ustawa o WOH a planowanie przestrzenne
Publication11 maja Sejm uchwalił budzącą wiele emocji Ustawę o tworzeniu i działaniu wielkopowierzchniowych obiektów handlowych (o WOH). Zgodnie z uchwaloną definicją wielkopowierzchniowym obiektem handlowym jest obiekt o powierzchni sprzedaży powyżej 400 m2, prowadzący jakąkolwiek działalność handlową. Mimo iż Ustawie o WOH przyświecają szczytne cele racjonalizacji wykorzystania zasobów miejskich, jej skutki mogą w istocie negatywnie wpłynąć...
-
Characterisation of Escherichia coli isolates from the blood of haematological adult patients with bacteraemia: translocation from gut to blood requires the cooperation of multiple virulence factors
PublicationThe aim of the study was to investigate whether there are unique pathotypes of Escherichia coli capable of transmission from the gastrointestinal tract to the vascular bed. The study included E. coli strains isolated from clinical materials collected from 115 patients suffering from haematologic malignancies diagnosed with bacteraemia. The genotyping techniques established that 89 E. coli isolates from the blood had the same genotype...
-
Utilization of New Activated Carbon Derived from an Oak Leaves for Removal of Crystal Violet from Aqueous Solution
PublicationSignificant negative environmental impact has been noticed at the campus garden of Gdansk University of Technology , Poland, when the withered leaves of Quercus robur Oak Tree (OL) cover large areas at such places. In this regards,an attempt has been made to recycle the most abundant agricultural leaf waste into a high quality local activated carbon (LAC) for use in the decolorizating processes of dye industrial waste- water.
-
A random field-based simulational identification of possible levels of material imperfections of adhesive-bonded joints
PublicationRecently, structural adhesives have become significant in the shaping of structural elements, especially in thin-walled structures, where they replace or supplement traditional connection methods. However, adhesive-bonded joints are highly susceptible to internal structural imperfections due to their application technique and the nature of the adhesive. These material inconsistencies impact the strength parameters and the mechanical...
-
The Effect of Hyperbaric Storage on the Nutritional Value and Retention of Certain Bioactive Proteins in Human Milk
PublicationHuman milk (HM) contains the essential macronutrients and bioactive compounds necessary for the normal growth and development of newborns. The milk collected by human milk banks is stored frozen and pasteurized, reducing its nutritional and biological value. The purpose of this study was to determine the effect of hyperbaric storage at subzero temperatures (HS-ST) on the macronutrients and bioactive proteins in HM. As control samples,...
-
Research on assessment of bolted joint state using elastic wave propagation
PublicationThe work contains results of experimental investigation of elastic wave propagation in a bolted single-lap joint. Tests were carried out for the excitation perpendicular to the connection plane. In experimental studies, PZT transducers were used for both excitation and registration of ultrasonic waves. The analyses took into account varying contact conditions between the elements of the connection depending on the value of the...
-
Experimentation, material modelling and simulation of bonded joints with a flexible adhesive
PublicationThe paper deals with material parameters identification of a flexible adhesive and the numerical simulation of its behaviour in a single lap bonded joint. The material modelling was based on the simple shear and tensile-shear laboratory tests of the bonded joints made of thin aluminium and polyurethane plates with the flexible adhesive Terostat MS 9360 manufactured by Henkel AG & Co. The adhesive was considered as a hyperelastic...
-
Regulated assembly of lipopolysaccharide and sensing of its alterations in Escherichia coli
PublicationThis thesis describes mechanism of the regulation of the transcription of the rpoE gene encoding an essential RNA polymerase subunit in Escherichia coli. The RpoE regulates extracytoplasmic stress response regulon and is required to initiate transcription of genes, whose products are involvedin the folding of periplasmic proteins and synthesis and transport of outer membrane components. The transcriptional regulation of the rpoE...