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Phenotypic consequences of LYS4 gene disruption in Candida albicans
PublikacjaA BLAST search of the Candida Genome Database with the Saccharomyces cerevisiae LYS4 sequence known to encode homoaconitase (HA) revealed ORFs 19.3846 and 19.11327. Both alleles of the LYS4 gene were sequentially disrupted in Candida albicans BWP17 cells using PCR-based methodology. The null lys4Δ mutant exhibited lysine auxotrophy in minimal medium but was able to grow in the presence of L-Lys and α-aminoadipate, an intermediate...
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Binder-induced surface structure evolution effects on Li-ion battery performance
PublikacjaA comparative investigation on binder induced chemical and morphological evolution of Li4Ti5O12 electrodes was performed via X-ray photoemission spectroscopy, scanning electron microscopy, and electrochemical measurements. Composite electrodes were obtained using three different binders (PAA, PVdF, and CMC) with 80:10:10 ratio of active material:carbon:binder. The electrochemical performances of the electrodes, were found to be...
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Application of non-metal doped titania for inverted polymer solar cells
PublikacjaDomieszkowany jodem oraz domieszkowany jodem ditlenek tytanu uzyto jako warstwę buforową w ogniwie ITO/X-TiO2?P3HT-PCMB /MnO2/ Ag.Warswty X-TiO2 nalożono metodą spin-coatingu z zawiesiny nanocząstek w DMSO. Jednorodne struktury scharakteryzowane pod względem morfologii mikroskopowo metodą AFM. Charakterystyka prądowo-napięciowa urzdzeń wskazuje iż domieszkowany jodem TiO2 pozwala osiągnąć lepsze parametry PCE (1,67%) i prąd zwarcia...
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A New Coupler Concept for Contactless High-Speed Data Transmission Monitoring
PublikacjaThis paper presents a new concept of a couplerthat can be applied to high-speed data transmission contactlessmeasurements. The proposed approach is dedicated for differentialsignal transmission monitoring in microstrip coupled lineson printed circuit boards (PCBs). The coupler, produced on aseparate PCB, is overlayed on the transmission line with thedifferential signal and delivers decoupled differential signal tothe main measurement...
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5-Bromo-2′-deoxycytidine—a potential DNA photosensitizer
PublikacjaA double-stranded oligonucleotide, 80 base pairs in length, was multiply labeled with 5-bromo-2′-deoxycytidine (BrdC) using polymerase chain reaction (PCR). The modified oligonucleotide was irradiated with 300 nm photons and its damage was assayed by employing DHPLC, LC-MS and denaturing polyacrylamide gel electrophoresis (PAGE). Two types of damage were demonstrated, namely, single strand breaks (SSBs) and intrastrand cross-links...
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Persistent organic pollutants (POPs) as an indicator of surface water quality in the vicinity of the Polish Polar Station, Horsund
PublikacjaSurface waters were collected in the vicinity of the Polish Polar Station in Hornsund (Svalbard) to determine their concentration of various persistent organic pollutants (POPs), such as polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs). In addition, parameters such as pH, SEC and TOC were measured. In total, 15 samples of lake water from and 15 samples of stream water were analysed. The sampling took...
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Design of control algorithms for mobile robots in an environment with static and dynamic obstacles
PublikacjaThis article proposes the construction of autonomous mobile robots and designing of obstacle avoidance algorithms for them. Nowadays, mobile robots are gaining more and more popularity on the customer as well as industrial market, for example as automatic vacuum cleaners or lawnmowers. Obstacle avoidance algorithms play an important role in performance of this types of robots. The proposed algorithms were designed for builds with...
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Dopants and gas modifiers in ion mobility spectrometry
PublikacjaThe ion mobility techniques, including the most commonly used drift-tube ion mobility spectrometry (IMS) and differential mobility spectrometry (DMS), are used successfully for the detection of a wide range of organic compounds in the gas phase. In order to improve detection quality, admixtures are added to gas streams flowing through the detector. Dopants mostly prevent the ionization of interfering chemicals however, better detection...
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DYNAMIC ANALYSIS OF THE IMPACT OF VIBRATION ON HISTORICAL BUILDINGS - AN OWN PROJECT OF EXTERNAL STAIRCASE
PublikacjaThis article presents numerical simulations that concern modeling of interaction of the external staircase’s construction with the observation deck on historic water tower located at Gdansk University of Technology. The interaction occurring according to various methods of the staircase's construction support on the wall of the historic tower was compared. The external staircase that allows for entering onto the monument was subjected...
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Information Logistics as a Paradigm
PublikacjaThis paper presents a paradigm-based approach to information logistics. The approach deals with extracting concepts specific to information logistics in categories typical to paradigms. The resulting description should be easily connected to complementary components which are based on other paradigms, e.g. business process management and information systems development. Empirical part aims at checking and enhancing features typical...
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Social entrepreneurship and its hybridity determining resource challenges and chances
PublikacjaIn this conceptual paper, the author aims to grasp what are the particular challenges and opportunities in social entrepreneurship, in terms of resource access and mobilization, considering the hybrid nature of social enterprise. To this end, the author points to the nature of hybridity and discusses what advantages and disadvantages are inherent in financial, human and physical resources access for social enterprises as hybrid...
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Ring thruster - a preliminary optimisation study of ferrofluid seal and propeller
PublikacjaThe article reports the course of preliminary examination of a ferrofluid seal applied in liquid environment.A newly built research rig is described, and results of pressure tests performed for different shaft rotationsare discussed. Other studies carried out in the past only referred to the gas environment, while the presentexamination aims at finding an answer to the question whether magnetic liquids can be successfully used...
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Sound Art and Architecture: New Horizons for Architecture and Urbanism
PublikacjaThe article discusses the crossroad between art and architecture. It sketches out the theoretical and practical aspects of involving art into architecture and multisensory dimensions of space. The analysis is based on examples of innovative experimental activities for architecture: educational projects such as workshops, seminars and courses, combining art and architecture, with special emphasis on sound art, and the consequences...
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Comparison of selected off-the-shelf solutions for emotion recognition based on facial expressions
PublikacjaThe paper concerns accuracy of emotion recognition from facial expressions. As there are a couple of ready off-the-shelf solutions available in the market today, this study aims at practical evaluation of selected solutions in order to provide some insight into what potential buyers might expect. Two solutions were compared: FaceReader by Noldus and Xpress Engine by QuantumLab. The performed evaluation revealed that the recognition...
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Selected problems of sensitivity and reliability of a jack-up platform
PublikacjaThe paper deals with sensitivity and reliability applications to numerical studies of an off-shore platform model. Structural parameters and sea conditions are referred to the Baltic jack-up drilling platform. The study aims at the influence of particular basic variables on static and dynamic response as well as the probability of failure due to water waves and wind loads. The paper presents the sensitivity approach to a generalized...
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A prototype of electrochemical sensor for measurement of carbonyl compounds in air
PublikacjaThe paper presents the results of investigation on a prototype sensor for measurement of benzaldehyde and formaldehyde in air. Sensitivity, limit of quantification and coefficient of selectivity of the sensor have been determined as a function of membrane thickness and electrode type using square wave voltammetry (SWV) technique. The working and counter electrodes were made of platinum and gold. Ionic liquids 1-hexyl, 3-methylimidazolium...
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Corporate Entrepreneurship: A Literature Review and Future Research Perspectives
PublikacjaBackground. Nowadays, corporate entrepreneurship (CE) is perceived as an essential approach to boost the innovation and creativity within existing organizations for achieving higher opportunities in the market. This paper examines this concept, which has been largely discussed in the Anglo-Saxon world over the last thirty years. Like for many other phenomena, also in case of CE, this discussion has provided numerous conceptualizations,...
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Endothelial dysfunction due to eNOS uncoupling: molecular mechanisms as potential therapeutic targets
PublikacjaNitric oxide (NO) is one of the most important molecules released by endothelial cells, and its antiatherogenic properties support cardiovascular homeostasis. Diminished NO bioavailability is a common hallmark of endothelial dysfunction underlying the patho‑ genesis of the cardiovascular disease. Vascular NO is synthesized by endothelial nitric oxide synthase (eNOS) from the substrate L‑arginine (L‑Arg), with tetrahydrobiopterin...
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An Ultra-Low-Energy Analog Comparator for A/D Converters in CMOS Image Sensors
PublikacjaThis paper proposes a new solution of an ultra-low-energy analog comparator, dedicated to slope analog-to-digital converters (ADC), particularly suited for CMOS image sensors (CISs) featuring a large number of ADCs. For massively parallel imaging arrays, this number may be as high as tens-hundreds of thousands ADCs. As each ADC includes an analog comparator, the number of these comparators in CIS is always high. Detailed analysis...
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Interspecific and intraspecific variation in organochlorine pesticides and polychlorinated biphenyls using non-destructive samples from Pygoscelis penguins
PublikacjaAs humans are present in Antarctica only for scientific and tourism-related purposes, it is often described as a pristine region. However, studies have identified measurable levels of Persistent Organic Pollutants (POPs), such as organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs), in the Antarctic region. These are highly toxic anthropogenic compounds with tendency to travel long distances and reach remote environments, where...
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Theoretical study of the structure, energetics and vibrational frequencies of water-acetone and water-2-butanone complexes
PublikacjaKompleksy acetonu i 2-butanonu z 0-2 cząsteczkami wody połączonymi wiązaniem wodorowym z tlenem karbonylowym zbadano z wykorzystaniem teorii funkcjonału gęstości (DFT) na poziomie B3LYP/6-311++G(d,p). Obliczenia w fazie gazowej uzupełniono o optymalizacje w otoczeniu rozpuszczalnika, symulowanego za pomocą modelu polaryzowalnego kontinuum (PCM). Częstości drgań oscylacyjnych otrzymane na podstawie zależności empirycznej sa zgodne...
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Genetic features of clinical Pseudomonas aeruginosa strains
PublikacjaW grupie 62 izolatów klinicznych sprawdzono obecność 6 wybranych czynnikow wirulencji. We wcześniejszych badaniach z wykorzystaniem techniki ERIC - PCR wykazano, że w analizowanej grupie 49 (79%) to szczepy, o dużum podobieństwie genetycznym (ponad 62%), każdy z pozostałych 13 izolatów zaliczono do osobnego genotypu, o zdecydownie niższym stopniu pokrewieństwa. W przypadku wszystkich 49 szczepów z pierwszej grupy stwierdzono obecność...
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Atom-economic thiophosphoroselenenylations of C–H acid esters and amides
PublikacjaThree improved thiophosphoroselenenylation procedures of CHacids, including derivatives of malonic and acetyl-, phosphono-, 4-nitrophenyl- and 3-pyridylacetic acids, have been described and compared to previously reported thiophosphoroselenylation of diethyl malonate using bis(disopropoxyphosphinothioyl)diselenide alone or with the aid of methyl iodide. The use of iodine makes it possible to utilize both equivalents of the selenenylating...
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Spray-deposited carbon-nanotube counter-electrodes for dye-sensitized solar cells
PublikacjaCarbon nanotubes due to their catalytic properties are a promising alternative to platinum counter electrodes (CE) for dye-sensitized solar cells (DSSC). In this study, counter electrodes were made from double-walled carbon nanotube (DWCNT) ink using the spray printing technique and afterwards they were thermally treated at temperatures ranging from 120 to 300 °C. Morphology and structure was studied using scanning electron microscopy...
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Modification of structured bio‑carbon derived from spongin-based scaffolds with nickel compounds to produce a functional catalyst for reduction and oxidation reactions: Potential for use in environmental protection
PublikacjaThree different 3D fibrous-like NiO/Ni(OH)2/Ni‑carbonized spongin-based materials were prepared via a simple sorption–reduction method. Depending on the support used, the catalysts were composed of carbon, nickel oxide, nickel hydroxide and zero-valent nickel, with the surface content of the nickel-containing phase in the range 15.2–26.0 wt%. Catalytic studies showed promising activity in the oxidation of phenolic compounds in water...
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Chronic Rhinosinusitis—Microbiological Etiology, Potential Genetic Markers, and Diagnosis
PublikacjaChronic rhinosinusitis (CRS) is a significant public health problem. Bacterial colonization and impaired mucociliary clearance play a significant role in the inflammatory process. Several inflammatory pathways and host defense elements are altered in CRS, which may contribute to observed differences in the microbiome. To date, researching CRS has been difficult due to limited access to the studied tissue and a lack of available...
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Spray deposited carbon nanotubes counter electrodes for dye-sensitized solar cells
PublikacjaCarbon nanotubes due to their catalytic properties are a promising alternative to platinum counter electrodes (CE) for dye-sensitized solar cells (DSSC). In this study, counter electrodes were made from double-walled carbon nanotube (DWCNT) ink using the spray printing technique and afterwards thermally treated at temperatures ranging from 120 to 300 °C. Morphology and structure was studied using scanning electron microscopy and...
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Properties of recombinant trehalose synthase from Deinococcus radiodurans expressed in Escherichia coli
PublikacjaA trehalose synthase gene from Deinococcus radiodurans(DSMZ 20539)containing 1659 bp reading frame encoding 552 amino acids was amplified using PCR. The gene was finally ligated into pET30Ek/LIC vector and expressed after isopropyl β-d-thiogalactopyranoside induction in Escherichia coli (DE3) Rosetta pLysS. The recombinanttrehalose synthase (DraTreS) containing a His6-tag at the C-terminus was purified by metal affinity chromatography...
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Determination of Odour Interactions of Three-Component Gas Mixtures Using an Electronic Nose
PublikacjaThe paper presents an application of an electronic nose prototype comprised of six TGS-type sensors and one PID-type sensor to identify odour interaction phenomena in odorous three-component mixtures. The investigation encompassed eight odorous mixtures—toluene-acetone-triethylamine and formaldehyde-butyric acid-pinene — characterized by different odour intensity and hedonic tone. A principal component regression (PCR) calibration...
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Recovery and Management of Postindustrial Spaces in the City on Selected Examples od European Implementations
PublikacjaUndeveloped, neglected and then abandoned post-industrial areas often located in a short distance from city centers have become, on one hand a major problem for city stewards due to the formation of dead urban tissue disturbing the continuity and cohesion of urbanized areas, on the other hand are spaces with high potential for a good location. The article aims to formulate, from the architectural and urban perspective, an answer...
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Towards Extending Wikipedia with Bidirectional Links
PublikacjaIn this paper, we present the results of our WikiLinks project which aims at extending current Wikipedia linkage mechanisms. Wikipedia has become recently one of the most important information sources on the Internet, which still is based on relatively simple linkage facilities. A WikiLinks system extends the Wikipedia with bidirectional links between fragments of articles. However, there were several attempts to introduce bidirectional...
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Research-by-design Best Practices in Architectural Lighting Design: Defragmenting Research Theories for their Effective Use in the Practice of Architectural and Spatial Design After Dark
Publikacja"This paper aims to combine theory and applied science; academia and practice for the creative transformation of spaces after dark taking into account a consistent environmental awareness. It is based on the premise that design research in the realm of architectural lighting design should function as a development of practice, instead of being a distraction to it. Architectural lighting design is a field within architecture and...
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Mesh dependence study for numerical assessment of hydrodynamic characteristics of windsurfing fin
PublikacjaThe presented research aims to assess the drag coefficient and lift coefficient versus angle of attack curves for windsurfing fin. Special attention in the research was being paid to the evaluation of the stall angle value. The angle of incidence for which the stall occurs was searched, and the sensitivity of the solution for the mesh resolution was studied. The mesh resolution sensitivity analysis was done by systematically decreasing...
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Positive Risk of Creativity in Software Projects: an Expected Result, a Threat or an Opportunity?
PublikacjaPositive risk of creativity appears when there is an excess of creativity. This paper aims at exploring the concept of positive risk of creativity in software projects. It presents a taxonomy of positive risks as well as case studies of positive risks in diploma software projects. It also attempts to answer the question whether positive risks should be treated as expected results of applying creativity process, or they cause additional...
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THE EUROPEAN UNION AND JAPAN TOWARDS STRATEGIC PARTNERSHIP AGREEMENT AND ECONOMIC PARTNERSHIP AGREEMENT
PublikacjaThis study aims to reveal the evolution of EU-Japan relations towards comprehensive and fully formalized strategic partnership, the main determinants of that process, as well the importance of the political and economic alliance. In the evolution of EU-Japan relations, there were identified four stages - 1960-1990, 1991-2000, 2001-2010, 2011-2018 - which had led up to strategic partnership regulated by the framework political...
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Music Recommendation Based on Multidimensional Description and Similarity Measures . Rekomendacja muzyki na podstawie wielowymiarowego wektora cech i miar podobieństwa
PublikacjaThis study aims to create an algorithm for assessing the degree to which songs belong to genres defined a priori. Such an algorithm is not aimed at providing unambiguous classification-labelling of songs, but at producing a multidimensional description encompassing all of the defined genres. The algorithm utilized data derived from the most relevant examples belonging to a particular genre of music. For this condition to be met,...
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Electrochemical sensor for measurement of volatile organic compounds employing square wave perturbation voltage
PublikacjaThe paper presents the results of investigation on a prototype sensor for measurement of benzaldehyde in air. Sensitivity and limit of quantification of the sensor were determined for different internal electrolyte using the square wave voltammetry (SWV) as a detection technique. The working and counter electrodes were made of platinum. Ionic liquids 1-hexyl, 3-methylimidazolium chloride,1-hexyl, 3-methylimidazolium bis (trifluoromethanesulfonyl)...
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Mobile operators at war: opinion mining and sentiment analysis on social media
PublikacjaConsidering hermetic and very competitive market such as mobile operator ones, social media has become best alternative for contact with customer and gathering data and opinions. Different style of running social media profiles is giving different results. The research presented in this paper aims to show the number of responses gathered from polish Internet users and its sentiment for mobile operator brands. It also presents practical...
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MAlSim - Mobile Agent Malware Simulator
PublikacjaOne of the problems related to the simulation of attacks against critical infrastructures is the lack of adequate tools for the simulation of malicious software (malware). Malware attacks are the most frequent in the Internet and they pose a serious threat against critical networked infrastructures. To address this issue we developed Mobile Agent Malware Simulator (MAISim). The framework uses the technology of mobile agents and...
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Heuristic Method of Safe Manoeuvre Selection Based on Collision Threat Parameters Areas
PublikacjaThis paper is a continuation of papers dedicated to a radar-based CTPA (Collision Threat Parameters Area) display designed to support safe manoeuvre selection. The display visualizes all the ships in an encounter and presents situational overview from the own ship’s point of view. It calculates and displays information on unsafe or unrealistic own ship’s course & speed allowing a user to select a safe manoeuvre. So far only the...
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A solution of the integrated µBIST for functional and diagnostic testing in mixed-signal electronic embedded systems
PublikacjaMain problem of the paper is testing of analog circuits and blocks in mixed-signal electronic embedded systems (EESs), using the built-in self-test (BIST) technique. The integrated mBIST based on reusing signal blocks already present in an EES, such as processors, memories, ADCs, is presented. The novelty of the solution is the extended functionality of the mBIST. It can perform 2 testing functions: functional testing and fault...
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Komitet Chemii Analitycznej PAN i jego działalność : Historia i dzień dzisiejszy
PublikacjaIn the contemporary society analytical chemistry as a scientific discipline and its practical application, termed often ;analysis'', has many important aims, and its role still increases. The Committee, formed in 1955 as a Commission, during several decades has coordinated and supported the activity of Polish analytical chemists on the national and international fields. Its present role includes organization of conferences and...
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Interferometric Sensor of Wavelength Detuning Using a Liquid Crystalline Polymer Waveplate
PublikacjaOperation of a polarization interferometer for measurement of the wavelength changes of a tunable semiconductor laser was investigated. A lambda/8 waveplate made from liquid crystalline polymer is placed in one of interferometers’ arms in order to generate two output signals in quadrature. Wavelength was measured with resolution of 2 pm in the wavelength range 628–635 nm. Drift of the interferometer, measured in the period of 500...
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Sposoby wyznaczania granicy wykrywalności i oznaczalności
PublikacjaW pracy zestawiono podstawowe terminy i definicje dotyczące granicy wykrywalności i oznaczalności. Dokonano także przeglądu metod szacowania i obliczania granicy wykrywalności zgodnie z zaleceniami zawartymi w podstawowych przewodnikach dotyczących walidacji procedur analitycznych takich jak: EURA-CHEM czy ICH. Na przykładzie oznaczania zawartości polichlorowanych bifenyli w próbkach tłuszczu wieprzowego z wykorzystaniem techniki...
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Assessment of the Current Potential of Hydropower for Water Damming in Poland in the Context of Energy Transformation
PublikacjaThe present paper indicates that hydropower, including small hydropower plants (SHPs), may play a very important role in Poland’s energy transformation in the near future. The development of SHPs may also increase water resources in the steppe Poland. Additionally, the aim of the present research is to conduct the PEST analysis of SHPs in Poland, taking into account the SHP potential. For the first time, maps showing the power...
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Analysis of friction ridge evidence for trace amounts of paracetamol in various pharmaceutical industries by Raman spectroscopy
PublikacjaThe detection of potentially harmful substances presents a multifaceted challenge. On one hand, it can directly save lives, on the other, it can significantly aid and enhance police work, thereby increasing the effectiveness of investigations. The research conducted in this study primarily aims to identify paracetamol in fingerprints, considering situations involving direct contact of a person with paracetamol either chronically...
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Image Representation for Cognitive Systems Using SOEKS and DDNA: A Case Study for PPE Compliance
PublikacjaCognitive Vision Systems have gained significant interest from academia and industry during the past few decade, and one of the main reasons behind this is the potential of such technologies to revolutionize human life as they intend to work under complex visual scenes, adapting to a comprehensive range of unforeseen changes, and exhibiting prospective behavior. The combination of these properties aims to mimic the human capabilities...
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Fire-induced spalling of ultra-high performance concrete: A systematic critical review
PublikacjaUltra-high performance concrete (UHPC) is a novel concrete class characterized by a compressive strength of more than 150 MPa. One of the most significant drawbacks of employing UHPC is that is very low permeability owing to its great compactness of dense structure increases the risk of fire-induced spalling. It is challenging for fire safety and structural engineers to predict and analyze this issue due to the lack of widely accepted...
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Postprodukcja nagrania wideo z dzwiekiem dookolnym
PublikacjaOne of the aims of this paper is to present issues related to audio-video correlation. This is presented on the basis of a short film realization employing surround microphone techniques. First, some related works in the domain of sound and vision correlation are presented. Then assumptions concerning scene creation related to both audio and video are shortly described. Another objective is to discuss results of subjective tests...
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Integrated membrane bioreactors modelling: A review on new comprehensive modelling framework
PublikacjaIntegrated Membrane Bioreactor (MBR) models, combination of biological and physical models, have been representing powerful tools for the accomplishment of high environmental sustainability. This paper, produced by the International Water Association (IWA) Task Group on Membrane Modelling and Control, reviews the state-of-the-art, identifying gaps for future researches, and proposes a new integrated MBR modelling framework. In...