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Search results for: TERMOSTABILNA L-AMYLAZA
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The psychrotrophic yeast Sporobolomyces roseus LOCK 1119 as a source of a highly active aspartic protease for the in vitro production of antioxidant peptides
PublicationA psychrotrophic yeast strain producing a cold-adapted protease at low temperature was classified as Sporobolomyces roseus. In standard YPG medium, S. roseus LOCK 1119 synthesized an extracellular protease with an activity of approximately 560 U/L. Optimization of medium composition and process temperature considerably enhanced enzyme biosynthesis; an approximate 70% increase in activity (2060 U/L). The native enzyme was purified...
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Water and chemical properties of hydrometeors over central european mountains
PublicationAtmospheric pollutants are transferred to the groundby the contribution of various types of hydrometeors. Because ofthe different techniques of measurement, comparative analysesbetween them are often neglected. Hence, the main goal is tocompare water volume and chemistry of different types ofhydrometeors and their role in both: water balance and pollutantsdeposition. The results of water input and atmospheric depositschemistry...
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Merging Proline:Xylitol Eutectic Solvent in Crosslinked Chitosan Pervaporation Membranes for Enhanced Water Permeation in Dehydrating Ethanol
PublicationThe scope of this research aims at merging a new deep eutectic mixture (DES) into a biopolymer-based membrane for a pervaporation application in dehydrating ethanol. Herein, an L-proline:xylitol (at 5:1) eutectic mixture was successfully synthesized and blended with chitosan (CS). A complete characterization of the hybrid membranes, in terms of morphology, solvent uptake, and hydrophilicity, has been conducted. As part of their...
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Polymorphism in the chemokine receptor 7 gene (CCR7) is associated with previous myocardial infarction in patients undergoing elective coronary angiography
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A Measurable Selector in Kadison’s Carpenter’s Theorem
PublicationWe show the existence of a measurable selector in Carpenter’s Theorem due to Kadison. This solves a problem posed by Jasper and the first author in an earlier work. As an application we obtain a characterization of all possible spectral functions of shift-invariant subspaces of L 2 (R d ) and Carpenter’s Theorem for type I ∞ von Neumann algebras.
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Experimental guinea pig model of dermatophytosis: a simple and useful tool for the evaluation of new diagnostics and antifungals
PublicationCelem badań było opracowanie prostego modelu zwierzęcego (świnki morskie) odpowiedniego do sprawdzania systemów diagnostycznych oraz terapii infekcji dermatofitów. Następujące zmienne były analizowane: przygotowanie skóry przez golenie versus użycie taśmy, testowy szczep Microsporum canis czy Trichophyton mentagrophytes jako czynnik etiologiczny, sposób inokulacji. Przebieg infekcji był oceniany poprzez ocenę zaczerwienienia i...
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Możliwości zastosowania procesu anammox do usuwania azotu amonowego ze ścieków zawierających sulfonamidy
PublicationProces Anammox zastosowano do usuwania azotu amonowego ze ścieków zawierających p-toluenosulfonamid. Stwierdzono, że jedynie bardzo wysokie stężenie tego związku (>100 mg/l) obniża wydajność i szybkość procesu. Nie stwierdzono pozytywnego efektu aklimatyzacji bakterii Anammox na szybkość i wydajność procesu
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Selectivity Tuning by Natural Deep Eutectic Solvents (NADESs) for Extraction of Bioactive Compounds from Cytinus hypocistis—Studies of Antioxidative, Enzyme-Inhibitive Properties and LC-MS Profiles
PublicationIn the present study, the extracts of Cytinus hypocistis (L.) L using both traditional solvents (hexane, ethyl acetate, dichloromethane, ethanol, ethanol/water, and water) and natural deep eutectic solvents (NADESs) were investigated in terms of their total polyphenolic contents and antioxidant and enzyme-inhibitive properties. The extracts were found to possess total phenolic and total flavonoid contents in the ranges of 26.47–186.13...
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Preliminary studies of the chemical composition and cytotoxic activity of Hottonia palustris herb
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Corrosion studies using potentiodynamic and EIS electrochemical techniques of welded lean duplex stainless steel UNS S82441
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The effect of the type of substrate and its surface treatment on the pull-off strength of gypsum plasters
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The influence of texturing of the surface of concrete substrate on its adhesion to cement mortar overlay
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Quantum metrology: Heisenberg limit with bound entanglement
PublicationQuantum entanglement may provide a huge boost in the precision of parameter estimation. However, quantum metrology seems to be extremely sensitive to noise in the probe state. There is an important still open question: What type of entanglement is useful as a resource in quantum metrology? Here we raise this question in relation to entanglement distillation. We provide a counterintuitive example of a family of bound entangled states...
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Backscattering properties of southern Baltic herring
PublicationReliable TS(L) relationship is required to improve acoustic algorithms of abundanceestimation of Baltic herring. The relationships, empirically obtained in different parts of theBaltic Sea, produce up to 8 dB – difference in the herring TS. In order to develop an accurateTS(L) relationship, it is important to analyze factors controlling the TS variability. Thepossible impact of the regional difference...
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Deep eutectic solvent (DES) with silver nanoparticles (Ag-NPs) based assay for analysis of lead (II) in edible oils
PublicationThis paper presents an application of silver nanoparticles impregnated by Deep Eutectic Solvents (DES) as ultrasonication aided microextraction system for lead (II) determination in edible oils. The paper presents a systematic optimization of method parameters and examples of its application for analysis of real samples. Maximum recovery for lead (II) extraction was obtained for choline chloride and phenol with a 1:2 molar ratio....
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Green, simple analytical method for biogenic amines determination in fruit juice samples using salting-out assisted liquid-liquid microextraction and gas chromatography-mass spectrometry
PublicationSalting-out assisted liquid-liquid microextraction (SALLME) was integrated with the derivatization procedure to establish a one-step sample pre-treatment approach for rapid analysis of 14 biogenic amines (BAs) in fruit juices. The methodology consists of salting-out of analytes, derivatization with ethyl chloroformate (ECF), extraction with ethyl acetate (EtAc), and the analysis of the derivatized BAs using gas chromatography-mass...
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Formation of Protein Networks between Mucins: Molecular Dynamics Study Based on the Interaction Energy of the System
PublicationMolecular dynamics simulations have been performed for a model aqueous solution of mucin. As mucin is a central part of lubricin, a key component of synovial fluid, we investigate its ability to form cross-linked networks. Such network formation could be of major importance for the viscoelastic properties of the soft-matter system and crucial for understanding the lubrication mechanism in articular cartilage. Thus,the inter- and...
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Granulometric characterization of Arctic driftwood sawdust from frame sawing process
PublicationArctic driftwood can be used as an alternative source of wood as construction timber and furniture material, especially in Iceland and Greenland. The use of Arctic driftwood can help in the fight against climate change, by developing land reforestation processes and reducing the volume harvested wood from forests and sustainability of harvesting processes. In this paper the results of an analysis of the effect of long-term residence...
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A magnetic imprinted polymer nano-adsorbent with embedded quantum dots and mesoporous carbon for the microextraction of triazine herbicides
PublicationA magnetic molecularly imprinted polymer (MMIP) adsorbent incorporating amino-functionalized magnetite nanoparticles, nitrogen-doped graphene quantum dots and mesoporous carbon (MIP@MPC@NGQDs@ Fe3O4–NH2) was fabricated to extract triazine herbicides from fruit juice. The embedded magnetite nanoparticles simplified the isolation of the adsorbent from the sample solution. The N-GQDs and MPC enhanced adsorption by affinity binding...
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Effect of TiO2 Concentration on Microstructure and Properties of Composite Cu–Sn–TiO2 Coatings Obtained by Electrodeposition
PublicationIn this work, Cu–Sn–TiO2 composite coatings were electrochemically obtained from a sulfate bath containing 0–10 g/L of TiO2 nanoparticles. The effect of TiO2 particles on kinetics of cathodic electrodeposition has been studied by linear sweep voltammetry and chronopotentiometry. As compared to the Cu–Sn alloy, the Cu–Sn–TiO2 composite coatings show rougher surfaces with TiO2 agglomerates embedded in the metal matrix. The highest...
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Ammonia amendment promotes high rate lactate production and recovery from semi-continuous food waste fermentation
PublicationIn this study, a reliable approach using ammonia nitrogen was proposed to increase lactate production during semi-continuous food waste (FW) fermentation under mesophilic conditions. Both free ammonia nitrogen (FAN) and ammonium ion (NH4+-N) were present in mesophilic reactors, with a wide FAN/NH4+-N ratio variation due to the intermittent pH control. The investigation of responsible mechanisms revealed that the increased production...
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Letter to the Editor: Complete resonance assignments of the 'donor-strand' AfaD: The afimbrial invasin from Diffusely Adherent E. coli.
PublicationUstalono strukturę NMR rekombiantowego białka AfaD-dsc. Uzyskane wyniki są podstawą do badań rentgenograficznych struktury białek AfaD/DraD.
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Impact of abiotic stressors on nutrient removal and rhizomicrobiome composition in floating treatment wetland with Equisetum hyemale
PublicationFloating treatment wetlands (FTW) are receiving growing interest as a phyto-technology. However, there are significant research gaps regarding the actual role of plant species and plant-microbiome interactions. In this study, the nutrient uptake of Equisetum hyemale was examined in FTW microcosms under the influence of abiotic stressors: As (3 mg/L) and Pb (3 mg/L) as well as Cl− (300 and 800 mg/L) in reference to a control during...
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Influence of the boron doping level on the electrochemical oxidation of raw landfill leachates: advanced pre-treatment prior to the biological nitrogen removal
PublicationThe electrochemical oxidative treatment of landfill leachates (LLs) containing high amounts of ammonia nitrogen and organic matter was used as a promising method, prior to biological processes, to achieve the final effluent quality that would be acceptable by current regulations. The deposited boron-doped diamond electrodes (BDDs) with different boron doping concentrations (10000, 5000 and 500 ppm of B) were applied as anodes....
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Sequential treatment of landfill leachate by electrocoagulation/aeration, PMS/ZVI/UV and electro-Fenton: Performance, biodegradability and toxicity studies
PublicationThis study presents a systematic study on sequential treatment of highly resistant landfill leachate by electrocoagulation (EC)/aeration, sulfate radical advanced oxidation process (SR-AOP) and electro-Fenton (EF). In case of SR-AOP, peroxymonosulfate (PMS) catalyzed by zero valent iron (ZVI) and ultraviolet irradiation (UV) system was developed. Treatment process was optimized in respect to COD removal. Analysis of results revealed...
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Determination of metals in water samples by atomic absorption spectrometry and flame emission spectrometry
PublicationMetal species in environmental waters are relevant to characterize the metal toxicity, the mobility between different environmental compartments, and for remediation purposes in case of contamination. The concentration of many trace metals in natural water samples are generally at µg/l level. The paper describes determination of metals in water samples based on flame atomic emission and flame atomic absorption spectrometry.
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Equitable coloring of corona multiproducts of graphs
PublicationWe give some results regarding the equitable chromatic number for l-corona product of two graphs: G and H, where G is an equitably 3- or 4-colorable graph and H is an r-partite graph, a cycle or a complete graph. Our proofs lead to polynomial algorithms for equitable coloring of such graph products provided that there is given an equitable coloring of G.
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Hydrophilic domain II of Escherichia coli Dr fimbriae facilitates cell invasion
PublicationStosując techniki mutagenezy ukierunkowanej oraz mikroskopii elektronowej stwierdzono, że hydrofilowa domena II białka podjednostkowego DraE fimbrii jest odpowiedzialna za inwazję komórki. Otrzymane wyniki są zgodne z interpretacją, że fenotypy fimbrii Dr wiązania i inwazji komórki HeLa mogą być oddzielone.
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''Computing with words'' concept applied to musical instrument recognition. W: [CD-ROM] International Symposium of Musical Acoustics. ISMA MEXICO CITY. Mexico City, 9-13 December 2002. Mexico City: Escuela Nacional de Musica UNAM**2002, 8 s. 3 rys. 3 tab. bibliogr. 25 poz. Automatyczne rozpoznawanie klas instrumentów muzycznych w oparciu o wyraże- nia opisujące barwę dźwięku.
PublicationW referacie przedstawiono nowy sposób automatycznego przetwarzania danychmuzycznych w oparciu o paradygmat zaproponowany przez L. Zadeha. Pozwala tona automatyczne rozpoznawanie klas instrumentów muzycznych wykorzystując o-pis słowny barwy dźwięku. Przedstawiono system realizujący automatyczną kla-syfikację instrumentów muzycznych oparty o metodę zbiorów przybliżonych ilogikę rozmytą.
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Własności kwantowe przemian energetycznych zachodzących w silnikach o zapłonie samoczynnym = Quantum properties of energy transformation in diesel engines
PublicationW pracy uzasadniono, że w badaniach własności energetycznych silników spalinowych o zapłonie samoczynnym należy uwzględnić istniejącą w ich eksploatacji przypadkowość i nieprzewidywalność zdarzeń. Nawiązano do osiągnięć mechaniki kwantowej wskazując na wynikający z nich postulat, że wielkości nazywane komplementarnymi, mają istotną własność, która polega na tym, że niemożliwy jest jednoczesny i zarazem dokładny pomiar ich wartości....
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GC-FID determination of C2-C4 aliphatic monocarboxylic acids in aqueous samples preceded by solvent extraction
PublicationEkstrakcja za pomocą eteru metylo-tert-butylowego (MTBE) została wykorzystana do izolacji krótkołańcuchowych alkanowych kwasów monokarboksylowych (2-4 atomy węgla w cząsteczce) z próbek o matrycy wodnej. Całkowita wydajność dwukrotnej ekstrakcji z próbek wodnych kwasów: octowego, propionowego i masłowego wynosiła odpowiednio ok. 55% , ok. 90% i ponad 90%. Wydajności ekstrakcji są porównywalne z wartościami uzyskiwanymi w przypadku...
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Development and validation of an eco-compatible UV–Vis spectrophotometric method for the determination of Cu2+ in aqueous matrices
PublicationCu 2+ are ubiquitous ions in the ecosystem and are responsible of serious environmental pollution. Indeed, the development of sensitive methods for Cu 2+ detection is an urgent demand. In this work, we proposed a new spectrophotometric method for Cu 2+ determination in different water matrices (distilled water, drinking water, wastewater, and river water). The method employs a bio-based organic ligand namely tetrasodium iminodisuccinate...
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Chapter 13. Immunological determination of the pharmaceutical diclofenac in environmental and biological samples
PublicationOpracowano wysoce specyficzną i czułą metodę immunologiczną oznaczania przeciwzapalnego leku diklofenaku. Jest to jeden z najczęściej wykrywanych farmaceutyków w środowisku. Granicę wykrywalności oraz wartość IC50 określono na poziomie 6 ng/L oraz 60 ng/L dla wody wodociągowej, odpowiednio. W badaniu porównawczym, stosując test ELISA oraz GC-MS określono poziomy stężeń diklofenaku w wodach ściekowych z 21 oczyszczalni ścieków i...
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New Trends in Sample Preparation Techniques for the Analysis of the Residues of Pharmaceuticals in Environmental Samples
PublicationPharmaceutical residues in the environment is a field of special interest due to the adverse effects to either human health or aquatic and soil environment. Pharmaceuticals have been already detected in underground, surface and wastewaters, soils, manure and sediments. The growing awareness of environmental pollution arising from human activity forces the need for their comprehensive determination. Environmental samples are complex...
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Fracture Toughness and Shear Yield Strength Determination for Two Selected Species of Central European Provenance
PublicationWhen offcut of wood is formed by shearing, Atkins’s analyses of sawing processes can be applied. Using this modern approach, it is possible to determine the fracture toughness and shear yield strength of wood. This model is only applicable for the axial-perpendicular cutting direction because both of these parameters are suitable for the given direction of cutting edge movement and cannot be considered material constants. Alternatively,...
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Immediate and late benefits of treating very elderly people with hypertension: results from active treatment extension to Hypertension in the Very Elderly randomised controlled trial
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Determination of tributyltin in marine sediment: Comit� Consultatif pour la Quantit� de Mati�re (CCQM) pilot study P-18 international intercomparison
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Homoclinic orbits for a class of singular second order Hamiltonian systems in ℝ3
PublicationWe consider a conservative second order Hamiltonian system \ddot{q}+ ∇V(q)=0 in R3 with a potential V having a global maximum at the origin and a line l ∩ {0} = ∅ as a set of singular points. Under a certain compactness condition on V at infinity and a strong force condition at singular points we study, by the use of variational methods and geometrical arguments, the existence of homoclinic solutions of the system.
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Badania porównawcze i ocena biodegradowalnego oleju do układów tnących pilarek łańcuchowych ''Profesjonalny Pilarz BIO''
PublicationCelem przeprowadzonych badań było sprawdzenie i porównanie parametrów oraz właściwości smarnych i przeciwzużyciowych z olejem maszynomym L-AN 68 (produktem stosowanym kiedyś w gospodarce leśnej do smarowania układów tnących pilarek łańcuchowych) oraz olejem Vexol Eko - biodegradowalnym produktem do smarowania prowadnic i łańcuchów pił, używanym obecnie przez OTL Świebodzin.
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The effect of surfactants on activated sludge process
PublicationBadano wpływ anionowego surfaktantu (SDBS) na proces biologicznego oczyszczania ścieków metoda osadu czynnego. Stwierdzono, że stężenie SDBS powyżej 50 mg/l miało wyraźny wpływ na przemiany związków azotu i fosforu w czasie oczyszczania ścieków. Wpływ badanego związku na usuwanie związków organicznych (wyrażonych jako ChZT) był mniej wyraźny.
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Organic pollutants photodegradation increment with use of TiO2 nanotubes decorated with transition metals after pulsed laser treatment
PublicationAmong various titanium(IV) oxide (TiO2, titania) structures, 1D nanotubes (TiO2 NTs) produced during the two-electrode anodization process, are extensively utilized in sensors or supercapacitors as well as in photo(electro)catalytic water splitting. However, due to wide bandgap and fast electron-hole recombination additional modifications, mostly concerned on materials surface, are required. According to the recent research, TiO2...
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Thermodynamics of new piroxicam derivatives in terahertz light
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Copper containing protein sites - structure, properties and models
PublicationEnzymy organizmów żywych katalizują wiele reakcji stosowanych w przemyśle chemicznym. Przykładem potencjalnego zastosowania enzymu w produkcji jest synteza L-Dopy przy udziale tyrozynazy. Ze względu na możliwe praktyczne zastosowanie w katalizie, otrzymano i opisano wiele prostych modeli miejsc aktywnych enzymów. W artykule opisano niektóre modele białek zawierających miedź.
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Wyznaczanie rozdzielczości pomiarów widm Ramana
PublicationOpisano parametry wpływające na rozdzielczość pomiarów widm Ramana. Następnie przedstawiono sposób wyznaczania rozdzielczości dla przykładowego układu spektrometru, wykorzystywanego w projekcie badawczym budowy przenośnego spektrometru Ramana. Wyniki analizy przeprowadzonej na podstawie prążków występujących w kilku pomierzonych widmach potwierdziły zgodność obliczeń z obserwowaną rozdzielczością pomiarów w przedziale 8,5-10,5...
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Mangiferin Affects Melanin Synthesis by an Influence on Tyrosinase: Inhibition, Mechanism of Action and Molecular Docking Studies
PublicationMangiferin is a strong antioxidant that presents a wide range of biological activities. The aim of this study was to evaluate, for the first time, the influence of mangiferin on tyrosinase, an enzyme responsible for melanin synthesis and the unwanted browning process of food. The research included both the kinetics and molecular interactions between tyrosinase and mangiferin. The research proved that mangiferin inhibits tyrosinase...
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Endothelial Dysfunction Driven by Hypoxia—The Influence of Oxygen Deficiency on NO Bioavailability
PublicationCardiovascular diseases (CVDs) are the leading cause of death worldwide. The initial stage of CVDs is characterized by endothelial dysfunction, defined as the limited bioavailability of nitric oxide (NO). Thus, any factors that interfere with the synthesis or metabolism of NO in endothelial cells are involved in CVD pathogenesis. It is well established that hypoxia is both the triggering factor as well as the accompanying factor...
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Rola mikroorganizmów przy wpracowywaniu kwarcowych złóż odmanganiających
PublicationCelem badań prezentowanych w publikacji było określenie znaczenia bakterii utleniających mangan w okresie wpracowywania świeżych złóż kwarcowych (przeznaczonych do usuwania manganu z wód podziemnych), tj. w procesie formowania się katalitycznej warstwy wyższych tlenków manganu.Stwierdzono, że zasiedlenie złóż filtracyjnych bakteriami utleniającymi mangan może mieć decydujące znaczenie dla procesu wpracowywania się filtrów. Już...
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Disinfection characteristics of an advanced rotational hydrodynamic cavitation reactor in pilot scale
PublicationHydrodynamic cavitation is a promising technique for water disinfection. In the present paper, the disinfection characteristics of an advanced hydrodynamic cavitation reactor (ARHCR) in pilot scale were studied. The effects of various flow rates (1.4–2.6 m3/h) and rotational speeds (2600–4200 rpm) on the removal of Escherichia coli (E. coli) were revealed and analyzed. The variation regularities of the log reduction and reaction...
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Rearrangements of the nitrifiers population in an activated sludge system under decreasing solids retention times
PublicationDue to the key role of nitrite in novel nitrogen removal systems, nitrite oxidizing bacteria (NOB) have been receiving increasing attention. In this study, the coexistence and interactions of nitrifying bacteria were explored at decreasing solids retention times (SRTs). Four 5-week washout experiments were carried out in laboratory-scale (V=10 L) sequencing batch reactors (SBRs) with mixed liquor from two full-scale activated sludge...
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Fluorescent analogs of trypsin inhibitor SFTI-1 isolated from sunflower seeds-synthesis and applications
PublicationThis article describes the synthesis and enzymatic study of newly synthesized analogs of trypsin inhibitors SFTI-1 that were fluorescent labeled on their N-terminal amino groups. Two fluorescent derivatives of benzoxazole (3-[2-(4-diphenylaminophenyl) benzoxazol-5-yl]-L-alanine[(4NPh(2)) Ph] Box-Ala and 3-[2-(2', 4', 5'-trimethoxyphenyl) benzoxazol-5-yl]-l-alanine-[2,4,5-(OMe)3Ph] Box-Ala) were used as efficient fluorescent labels....