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Search results for: SERINE PROTEINASE
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Possibility of valuation of operation of marine diesel engines
PublicationW artykule zaproponowano interpretację wartościującą działania okrętowych silników spalinowych, które (podobnie jak przedstawione mechanice klasycznej działania Hamiltona i Maupertiusa oraz działanie wynikające ze zmiany pędu ciała) jest rozpatrywane jako wielkość fizyczna o jednostce miary zwanej dżulosekundą [dżulsekunda]. Przedstawiono oryginalną metodę analizy i oceny działania silników o zapłonie samoczynnym w ujęciu energetycznym...
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Path Loss Modelling for Location Service Applications
PublicationThe aim of this paper is the path loss modeling for the radiolocation services in radiocommunication networks, particularly in cellular networks. The main results of the measurements obtained in the physical layer of the UMTS are introduced. A new method for the utilization of the multipath propagation phenomenon to improve the estimation of the distance between the mobile station (MS) and the base station (BS) is outlined. This...
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Evaluation of Workflow Runtime Platforms in Service Composition
PublicationTypically, workflow applications are constructed from basic functionalities that may be realized by alternative services deployed in heterogeneous runtime platforms. Depending on workflow structure and selection of services, the applications differ in attributes such as price, Quality of Service (QoS) and others. In the paper, we propose a method of evaluation of workflow runtime platforms using Data Envelopment Analysis. We present...
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Experimental and Numerical Study on Pounding of Structures in Series
PublicationPounding between structures in series during earthquakes may cause serious damage in the structural elements. The aim of this paper is to show the results of an experimental and numerical study that is focused on pounding between more than two structures which may be described as “structures in series”. In this study, the shaking table experiments, as well as the numerical analyses, were performed using three tower models including...
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Application of Fast Cameras to String Vibrations Recording
PublicationA hardware and software solution for guitar string vibration measurement by fast cameras is described. Orthogonal setup for 3D image acquisition is proposed capable to capture several thousand image frames per second. Dedicated image processing algorithm was developed and described in the paper, aimed at tracking the movement of some selected points along the string. Fast and accurate tracking results provided a detailed information...
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Chalcogen bonding interactions in a series of aromatic selenocyanates
PublicationSelenium atoms in aromatic selenocyanates are characterized by the occurrence of two σ-holes, a stronger one in the prolongation of the NC–Se bond and a weaker one in the prolongation of the Ar–Se bond. The crystal structures of several bis(selenocyanato) derivatives, prepared by a method originally developed for ortho bis-substituted derivatives, illustrate very well this difference, with a short NC–Se⋯NC ChB interaction organizing...
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Impact of SDN Controller’s Performance on Quality of Service
PublicationSoftware Defined Networking is a paradigm in network architecture; that is quickly becoming commonplace in modern telecommunication systems. It facilitates network customization for the requirements of different applications and simplifies the implementation of new services. Since its proposal, a significant evolution in its functionality has occurred. However, this development brought along problems of efficiency and performance,...
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Service-based Resilience for Embedded IoT Networks
PublicationEmbedded IoT networks are the backbone of safety-critical systems like smart factories, autonomous vehicles, and airplanes. Therefore, resilience against failures and attacks should be a prior concern already in their design stage. In this study, we introduce a service-based network model as an MILP optimization problem for the efficient deployment of a service overlay to the embedded network by meeting QoS and resilience requirements....
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Digital phase-location system for marine applications
PublicationThis paper presents concept and implementation of digital phase-location system, designed as a navigational aid for marine applications. Main feature of proposed system is the ability to work in both synchronous mode, with one master station and set of slave stations synchronized with master, and in asynchronous mode with independent clocking of all stations.
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Mobile Laser Scanning Calibration on a Marine Platform
PublicationThe article describes the method of calibration of the Riegl VMZ-400 mobile scanning system on a floating platform and an experiment aimed at conducting measurements from a previously calibrated instrument. The issue of factors affecting the quality of measurements was discussed. Mobile laser scanning is an increasingly popular measurement solution, but it is still innovative on a European scale. The use of a floating platform...
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Sounding of Layered Marine Bottom - Model Investigations
PublicationThe necessary actions connected with interpretations of examining of physical properties of sea bottom structure is developing of proper procedure of modeling of the layered structure. The implementation of the acoustic waves for the determination of seabed stratification requires a precise diagnosis of the topic from both the theoretical and practical side. Little differences between the parameters of sediments, as well as irregular...
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Design of a Multidomain IMS/NGN Service Stratum
PublicationThe paper continues our research concerning the Next Generation Network (NGN), which is standardized for delivering multimedia services with strict quality and includes elements of the IP Multimedia Subsystem (IMS). A design algorithm for a multidomain IMS/NGN service stratum is proposed, which calculates the necessary CSCF servers CPU message processing times and link bandwidths with respect to the given maximum values of mean...
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TIME SERIES DATA FOR 3D FLOOD MAPPING
PublicationThanks to the ability to collect information about large areas and with high frequency in time areas threatened by floods can be closely monitored. The effects of flooding are socio-economic losses. In order to reduce those losses, actions related to the determination of building zones are taken. Moreover, the conditions to be met by facilities approved for implementation in such areas are determined. Therefore, satellite data...
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Data visualization of marine objects on digital maps
PublicationThe paper presents the implementation of two multithreaded applications for data visualization of marine objects written in C#, designed to run on operator consoles with 32-bit or 64-bit Windows 7 OS. The article describes the most important functionality and features of the developed C# .NET user controls for data visualization on digital maps and in the configurable tables.
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Photoelectron spectroscopy of a series of acetate and propionate esters
PublicationThe electronic state and photoionization spectroscopy of a series of acetate esters: methyl acetate, isopropyl acetate, butyl acetate and pentyl acetate as well as two propionates: methyl propionate and ethyl propionate, have been determined using vacuum-ultraviolet photoelectron spectroscopy. These experimental investigations are complemented by ab initio calculations. The measured first adiabatic and vertical ionization energies...
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Influence of the jitter buffer on the quality of service VoIP.
PublicationVoIP (Voice over IP) is a modern service with enormous potential for yet further growth. It uses the already available and universally implemented IP transport platform. One significant problem, however, is ensuring the Quality of Service, abbreviated QoS. This paper addresses exactly that issue. In an extensive investigation the influence of jitter buffers on QoS is being examined in depth. Two implementations, namely a passive...
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Digital Public Service Innovation: Framework Proposal
PublicationThis paper proposes the Digital Public Service Innovation Framework that extends the "standard" provision of digital public services according to the emerging, enhanced, transactional and connected stages underpinning the United Nations Global e-Government Survey, with seven example "innovations" in digital public service delivery -- transparent, participatory, anticipatory, personalized, co-created, context-aware and context-smart....
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A wireless network for off-shore marine research
PublicationThis paper presents functionality, the most important technology developments and potential use cases of digital communication system, developed to facilitate selected tasks commonly required during off-shore operations and marine research, including: broadband information sharing between platforms isolated from an on-shore communication infrastructure, acquisition of large data-sets from variety of off-shore...
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Investigation of noises in the EPN weekly time series
PublicationThe constantly growing needs of permanent stati ons’ velocities users cause their stability level to increase. To this research we included more than 150 stations located across Europe operating within the EUREF Permanent Network (EPN) w ith weekly changes in the ITRF2005 reference frame. The obvious long-range dependencies in the stochastic part of GPS time series were p roven by Ljung-Box...
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Extending Service Selection Algorithms with Interoperability Analysis
PublicationApplication development by integration of existing, atomic services reduces development cost and time by extensive reuse of service components. In Service Oriented Architecture, there exist alternative versions of services supplying the same functionality but differing in Quality of Service (QoS) attributes, which enables developers to select services with optimal QoS. Existing algorithms of service selection focus on the formal...
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The importance and availability of marine certified reference materials
PublicationTraceability and reliable results are the two pillars of analytical methods; certified reference materials (CRMs) meet this requirement. ISO 17034:2016 credentials provide brief information on general requirements for the competence of Reference Material Producers (RMPs). The different types of CRMs have been produced in recent years for chemical analysis in food, water, soil, and sediment matrices in recent years. This review...
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The essence of marine and coastal space – an interdisciplinary perspective
PublicationSea space has been undergoing a profound transformation. Although it retains its inspirational function in arts, literature and philosophy, it has been gaining new anthropogenic dimensions in eco-nomics and urban planning as a source of satisfying human needs i.e. the provision of harmony, beauty, off-shore energy, and biotech substances. Therefore, in this paper marine space is analyzed from a mul-tidimensional perspective of...
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Sources and Fate of PAHs and PCBs in the Marine Environment
PublicationThe assessment of a hazard resulting from the pollution of the environment by chemical compounds is in principle limited to the determination of their concentrations in its various compartments. But for solving many problems in this context, knowledge of the emission sources, transport pathways, and sites of deposition is of great benefit. By far the largest amounts of pollutants, regardless of where they were discharged, end up...
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Traffic risk modelling using time-series
PublicationW referacie przedstawiono metodę prognozowania ryzyka w ruchu drogowym powstałą na bazie analizy szeregów czasowych. W jej oparciu dla danych o liczbie śmiertelnych ofiar wypadków drogowych w Polsce w latach 1989-2000 zbudowano model i wykonano prognozę rozwoju trendu w przyszłości.
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Water hammer analysis in two pipes in series
PublicationW pracy przedstawiono wyniki badań laboratoryjnych przepływu nieustalonego w rurociągu skokowo zmiennej średnicy. Dokonano porównania czynników pomiarów oraz obliczeń uderzenia hydraulicznego zmodyfikowaną metodą elementów skończonych.
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Evaluation of IEEE 802.11E extensions for quality of service
PublicationZaprezentowano założenia wersji "e" standardu 802.11, wspierającej obsługę zróżnicowanych, pod względem potrzeb QoS, strumień ruchu. Przeprowadzono różnorodne badania symulacyjne dla opcji EDCF oraz HCF tego standardu. Wskazano na mankamenty oraz zalety nowych rozwiązań.
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Outline of marine piston engines' technology development.
PublicationW referacie przedstawiono najistotniejsze wydarzenia mające wpływ na produkcję i rozwój silników o zapłonie samoczynnym. Zasygnalizowano najistotniejsze osiągnięcia w rozwoju tych silników i podano nazwy firm, w których one zostały zastosowane. Podano także najważniejsze fakty związane z produkcją silników w Polsce.
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Marine turbines: cogeneration and combined propulsion systems.
PublicationOmówiono główne aspekty kombinowanych siłowni okrętowych, zwracając szczególną uwagę na układy parowo-gazowe oraz skojarzone wytwarzanie pracy mechanicznej i ciepła. Przedstawiono perspektywy rozwoju tego napędu
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Traffic fatalities modelling using time-series.
PublicationReferat zawiera opis jednaj z metod analizowania trendów bezpieczeństwa ruchu drogowego opartej na teorii szeregów czasowych. Przedstawiono w nim aplikację tej metody do badania związku pomiędzy liczbą śmiertelnych ofiar wypadków drogowych w Polsce w latach 1991-2003 a wielkością bezrobocia w tym czasie.
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XXIV Aukcja Wielkie Serce
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XXVIII Aukcja Wielkie Serce
PublicationAukcja to wyjątkowa okazja dla miłośników i kolekcjonerów polskiej sztuki współczesnej. Kolejny raz będzie można obcować z dziełami artystów, którzy tworzą kanon, są „żywą encyklopedią” grafiki i malarstwa polskiego. Od początku lat 90. ubiegłego stulecia, organizatorzy aukcji szczycą się i zaufaniem najwybitniejszych polskich twórców. Pieniądze z licytacji ich dzieł, jak co roku, przekazane zostaną na rzecz wychowanków Specjalnego...
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Optimized procedure of extraction, purification and proteomic analysis of nuclear proteins from mouse brain
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An animal model of the procognitive properties of cysteine protease inhibitor and immunomodulatory peptides based on colostrum.
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The role of the LB structural loop and its interactions with the PDZ domain of the human HtrA3 protease
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The LA Loop as an Important Regulatory Element of the HtrA (DegP) Protease from Escherichia coli
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Electrodes Based on a Titanium Dioxide Nanotube–Spherical Silver Nanoparticle Composite for Sensing of Proteins
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Expression of INHβA and INHβB proteins in porcine oocytes cultured in vitro is dependent on the follicle size
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Elastic-contractile model proteins: Physical chemistry, protein function and drug design and delivery
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Efficient parallel algorithms in global optimization of potential energy functions for peptides, proteins, and crystals
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Post mortem development of meat quality as related to changes in cytoskeletal proteins of chicken muscles
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Identification and properties of the psychrophilic bacterium Desulfolatea psychrophila single-stranded DNA binding proteins
PublicationTo study the biochemical properties of SSB from Desulfolatea psychrophila (DpsSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 426 nucleotides encoding SSB protein of 142 amino acids with a calculated molecular mass of 15.6 kDa and it is the smallest known bacterial SSB protein indentified to date. The amino-acid sequence...
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RecA Proteins from Deinococcus geothermalis and Deinococcus murrayi - Cloning, Purification and Biochemical Characterisation
PublicationEscherichia coli RecA plays a crucial role in recombinational processes, the induction of SOS response and mutagenic lesion bypasses. It has also been demonstrated that RecA protein is indispensable when it comes to the reassembly of shattered chromosomes in gamma-irradiated Deinococcus radiodurans, one of the most radiation-resistant organisms known. Moreover, some functional differences between E. coli and D. radiodurans RecA...
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Interactions of telomeric proteins with nucleic acids: sequence recognition on intact and oxidatively damaged telomeres
PublicationTelomeres are complex nucleoprotein assemblies that play a vital role in the maintenance of functional ends of linear chromosomes. Telomeric DNA, composed of tandem repeats of the 5'-TTAGGG-3' motif, solves the so-called end replication problem: as chromosomes shorten with each cell division, no information is lost, and the telomere can be re-extended. In the cell, many protein factors regulate telomere length, nuclear positioning...
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The Effect of Hyperbaric Storage on the Nutritional Value and Retention of Certain Bioactive Proteins in Human Milk
PublicationHuman milk (HM) contains the essential macronutrients and bioactive compounds necessary for the normal growth and development of newborns. The milk collected by human milk banks is stored frozen and pasteurized, reducing its nutritional and biological value. The purpose of this study was to determine the effect of hyperbaric storage at subzero temperatures (HS-ST) on the macronutrients and bioactive proteins in HM. As control samples,...
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Optimization of parallel implementation of UNRES package for coarse‐grained simulations to treat large proteins
PublicationWe report major algorithmic improvements of the UNRES package for physics-based coarse-grained simulations of proteins. These include (i) introduction of interaction lists to optimize computations, (ii) transforming the inertia matrix to a pentadiagonal form to reduce computing and memory requirements, (iii) removing explicit angles and dihedral angles from energy expressions and recoding the most time-consuming energy/force terms...
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Nanoparticle Tracking Analysis of Urinary Extracellular Vesicle Proteins as a New Challenge in Laboratory Medicine
PublicationUrinary extracellular vesicle (uEV) proteins may be used as specific markers of kidney damage in various pathophysiological conditions. The nanoparticle-tracking analysis (NTA) appears to be the most useful method for the analysis of uEVs due to its ability to analyze particles below 300 nm. The NTA method has been used to measure the size and concentration of uEVs and also allows for a deeper analysis of uEVs based on their protein...
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Organic solvents aggregating and shaping structural folding of protein, a case study of the protease enzyme
PublicationLow solubility of reactants or products in aqueous solutions can result in the enzymatic catalytic reactions that can occur in non-aqueous solutions. In current study we investigated aqueous solutions containing different organic solvents / deep eutectic solvents (DESs) that can influence the protease enzyme's activity, structural, and thermal stabilities. Retroviral aspartic protease enzyme is responsible for the cleavage of the...
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Interfacial water controls the process of adsorption of hyperactive antifreeze proteins onto the ice surface
PublicationA mechanism of interactions between the ice-binding surface of a hyperactive antifreeze protein molecule and the ice surface is proposed, involving the influence of water present between the two surfaces on the behavior of the approaching molecule. It is demonstrated that the interfacial water, even before its full solidification, can act as a factor that pushes away or pulls nearer the protein molecule to ensure its proper positioning....
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Effect of urea and glycine betaine on the hydration sphere of model molecules for the surface features of proteins
PublicationWater properties may significantly affect protein stability. Osmolytes are compounds that intrinsically affect water in many different ways and thus can influence proteins with this type of indirect mechanism. In this study, we characterize water properties in ternary solutions: model–water–osmolyte, with two model molecules: N-methylacetamide (NMA) and dimethyl sulfoxide (DMSO) and two osmolytes: glycine betaine (TMG)and urea....
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Two bacterial small heat shock proteins, IbpA and IbpB, form a functional heterodimer
PublicationSmall heat shock proteins (sHsps) are a conserved class of ATP-independent chaperones which in stress conditions bind to unfolded protein substrates and prevent their irreversible aggregation. Substrates trapped in sHsps-containing aggregates are efficiently refolded into native structures by ATP-dependent Hsp70 and Hsp100 chaperones. Most γ-proteobacteria possess a single sHsp (IbpA), while in a subset of Enterobacterales, as...