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Search results for: PCR CONTAMINATION
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Numerical simulation of threshold-crossing problem for random fields of environmental contamination
PublicationCelem artykułu jest analiza szacowania prawdopodobieństwa, że pole losowe zanieczyszczeń nie przekracza pewnej wartości w danej dwuwymiarowej przestrzeni. W analizie wykorzystano metodę modelowania stochastycznego wykorzystując procedurę symulacji warunkowej. Opisany przykład praktycznego zastosowania metody dotyczy pola zanieczyszczenia metalami ciężkimi gruntu w regionie gdańskim.
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Modified and pristine biochars for remediation of chromium contamination in soil and aquatic systems
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Airborne peptidoglycans as a supporting indicator of bacterial contamination in a metal processing plant
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Soil and groundwater fecal contamination as a result of sewage sludge land application.
PublicationThe release of sludge-born bacteria and their further transport in subsurface was studied. The migration of bacteria was investigated in column experiments which were carried out under the conditions corresponding to naturally occurring extreme rainfall. Coal fly ash as well as coarse and medium grained sand, which properties are similar to the soils present in degraded areas, were used as column beds. Sewage sludge was applied...
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Partial characterization of proteins from mussel Mytilus galloprovincialis as a biomarker of contamination
PublicationPrzedmiotem zainteresowania były:- funkcjonalne i przeciwutleniające właściwości białek małża Mytilus galloprovincialis,- poziom zawartości metali ciężkich (ołów i kadm) w tkankach.Badania przeprowadzono dla małży zebranych zarówno w zanieczyszczonych, jak i w czystych obszarach strefy brzegowej Morza Czarnego w regionie Warny (Bułgaria). W badaniach wykorzystano takie techniki analityczne jak: spektroskopia FTIR, fluorescencja,...
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Modyfikacje polimeraz DNA jako rozwiązania problemów w reakcjach PCR
PublicationReakcja PCR już od wielu lat jest podstawowym narzędziem stosowanym w biotechnologii molekularnej. Nadal napotykamy jednak liczne problemy podczas jej przeprowadzania związane z trudnymi matrycami, obecnością inhibitorów itp. Optymalizacja reakcji PCR jest więc niezbędna w celu poprawy wyników. Jednym z rozwiązań tych problemów może być modyfikowanie polimeraz DNA. W powyższym artykule opisano problemy i rozwiązania zależne polimerazy...
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Real-time PCR approach in dermatophyte detection and Trichophyton rubrum identification
PublicationDermatophytes are keratinophilic molds that infect human hair, nails and skin. Diagnosis of dermatophytosis is based on morphological, serological and biochemical features. However, identification is difficult and laborious due to similarities between microorganisms. Thus, there is considerable interest to develop mycological diagnostic procedures based on molecular biology methods. In this study, fast, two-step DNA extraction...
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Diagnostic PCR tests for Microsporum audouinii, M. canis and Trichophyton infections
PublicationSince traditional diagnosis of dermatophyte infections is slow, we present a rapid new pcr test for detection of trichophyton spp., microsporum canis and m. audouinii infections. the performance of the test was evaluated with: 58 dermatophyte isolates; 10 yeast, mould and human dna control samples; 25 routine specimens from patients suspected of having dermatophytosis; 10 hair specimens from guinea pigs experimentally infected...
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Influence of configurations of the beds on contaminations removal in hybrid constructed wetlands.
PublicationThree hybrid constructed wetlands in configuration HF-VF-HF in Poland and one with another configuration VF-HF-Pond in Gremany were analysed. These hybrid constructed wetlands provide the second stage biological treatment. They are located in Pommerania region, in Sarbsk, Wiklino and Wieszyno, and their capacities are 29.7, 18.6 and 24.5 m3/day, respectively. The analysed system in Germany in Waizenfeld (Gemeinde Pommelsbrunn,...
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The sources of contamination and reasons of damage of the flow part of the marine turbine engine in operation
PublicationThe aim of this paperis to show particular sensitivity of the flow part of the marine turbine engine to the presence of contaminations in the intake air. The contaminations form hard-to-remove deposits in the intervene channels that result in a reduced efficiency and performance of the engine. The paper classifies the contaminants according the source of their formation and their destructive force. The performed analyses and syntheses...
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Mercury contamination, a potential threat to the globally endangered aquatic warbler Acrocephalus paludicola
PublicationMercury (Hg) contamination is considered a global concern for humans and wildlife, and although the number of studies dealing with that issue continues to increase, some taxonomic groups such as small passerine birds are largely understudied. In this paper, concentration of mercury in the aquatic warbler (Acrocephalus paludicola) feathers, a globally threatened passerine species, was examined. The concentration differences between...
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Effects of Contamination with Gasoline and Diesel Oil on Shear Strength of Coarse Grained Soil
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Enhancement of PUR/PIR foam thermal stability after addition of Zostera marina biomass component investigated via thermal analysis and isoconversional kinetics
PublicationIn the present work, a thorough thermogravimetric (TG) analysis of bio-based polyurethane–polyisocyanurate (PUR–PIR) foams in both nitrogen and oxygen atmosphere is performed. A sustainable element of the foam is a biopolyol obtained via acid-catalyzed liquefaction of Zostera marina and Enteromorpha Algae biomass. Based on isoconversional analysis and apparent activation energies, several conclusions are obtained. In contradiction...
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Diagnostyka zakażeń Candida spp. oparta o amplifikację DNA techniką PCR
PublicationZakażenia wywoływane przez oportunistyczne grzyby z rodzaju Candida są poważnym problemem klinicznym. Niezbędne jest poszukiwanie nowych szybkich, czułych i pewnych metod do identyfikacji gatunkowej tych drobnoustrojów. W artykule przedstawiono opisane w literaturze metody diagnostyki molekularnej drożdżaków z rodzaju Candida oparte o amplifikację DNA techniką PCR.
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BDNF Val66Met Polymorphism, the Allele-Specific Analysis by qRT-PCR - a Novel Protocol
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Molecular typing of clinical isolates of Escherichia coli by a new PCR MP method
PublicationTypowanie epidemiologiczne bakterii jest rutynową procedurą stosowaną w badaniach zakażeń szpitalnych i wymaga stosowania odpowiednich metod o wysokim potencjale różnicującym. Przedstawiliśmy możliwości nowej techniki PCR MP w różnicowaniu szczepów E. coli. Rezultaty zostały porównane z wynikami uzyskanymi metodą PFGE.
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Molecular typing of clinical isolates of Escherichia coli by a new PCR MP method
PublicationTypowanie epidemiologiczne bakterii jest rutynową procedurą stosowaną w badaniach zakażeń szpitalnych i wymaga stosowania odpowiednich metod o wysokim potencjale różnicującym. Przedstawiliśmy możliwości nowej techniki PCR MP w różnicowaniu szczepów E. coli. Rezultaty zostały porównane z wynikami uzyskanymi metodą PFGE.
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Revista Internacional de Contaminacion Ambiental
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Methods of Assessment of Metal Contamination in Bottom Sediments (Case Study: Straszyn Lake, Poland)
PublicationThe concentrations of six metals (Zn, Cu, Pb, Ni, Cr, and Cd) were investigated in bottom sediments of Straszyn Lake (North Poland). This study was designed to determine a total content of metals and to assess their mobility and bioavailability. The sequential extraction was used to fractionate metals into five fractions: exchangeable, bound to carbonates, bound to Fe–Mn oxides, bound to organic matter, and residual. The evaluation...
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High resolution model for assessment of contamination by chemical warfare agents dumped in the Baltic Sea
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Potential threat to Polish lakes and reservoirs from contamination by objects of environmental flood risk
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Heavy Metals in Sediments of Urban Streams: Contamination and Health Risk Assessment of Influencing Factors
PublicationSediments of two urban streams in northern Poland outflowing to the Baltic Sea were assessed to explain the spatial variation in relation to urbanization level of the catchment, the role of retention tanks (RTs) and identification of pollution level. During the 3 month period of investigation sediment samples were collected from the inflow (IN) and outflow (OUT) of six RTs located on streams for flood protection. Six heavy metals...
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Sources of contamination in sediments of retention tanks and the influence of precipitation type on the size of pollution load
PublicationDensification of cities and urban population contributes to increased runoff and suspended solids and alteration of the urban water cycle. Nowadays, Blue-Green Infrastructure is promoted to increase a city’s resilience to floods; however, stormwater drainage systems, supported with retention tanks are still important in protecting urban areas against floods. Sediment accumulation in stormwater infrastructure relates to an issue...
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Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms
PublicationA significant number of DNA-based techniques has been introduced into the field of microorganisms’ characterization and taxonomy. These genomic fingerprinting methods were developed to detect DNA sequence polymorphisms by using general principles, such as restriction endonuclease analysis, molecular hybridization, and PCR amplification. In recent years, some alternative techniques based on ligation of oligonucleotide adapters before...
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PCR-RFLP assays for species-specific identification of fungi belonging to Scopulariopsis and related genera
PublicationFungi of the Scopulariopsis genus, commonly found in the environment, are opportunistic pathogens that can cause various types of human infections. So far, no efficient molecular method has been developed for species differentiation among Scopulariopsis and related genera. In order to advance this field, we have evaluated performance of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assays, based...
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Opracowanie zestawu diagnostycznego do genetycznego typowania szczepów bakteryjnych metodą PCR MP
PublicationOmówiono etapy tworzenia zestawu diagnostycznego przeznaczonego do genetycznego typowania szczepów bakteryjnych wykorzystującego procedurę PCR MP (PCR Melting Profiles). Dokonano optymalizacji warunków i walidacji metody w celu przystosowania jej do potrzeb epidemiologicznej oceny zakażeń szpitalnych. Opracowany zestaw stwarza możliwości zastosowania go w laboratoriach prowadzących rutynowo badania diagnostyczne.
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Nested PCR for the detection of Aspergillus species in maxillary sinus samples of patients with chronic sinusitis
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Identification of Leaf Rust Resistance Genes in Selected Wheat Cultivars and Development of Multiplex PCR
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ADSRRS-fingerprinting and PCR MP techniques for studies of intraspecies genetic relatedness in Staphylococcus aureus
PublicationW pracy pokazano użyteczność dwóch nowych metod do typowania molekularnego Staphylococcus aureus, ADSRRS-fingerprinting (Amplification of DNA fragments Surrounding Rare Restriction Sites) i PCR MP (PCR melting profile). Przebadano 37 szczepów Staphylococcus aureus izolowanych od pacjentów z czyracznością. Jako metodę referencyjną zastosowano REA-PFGE. Okazało się, że izolaty Staphylococcus aureus z nosa od niektórych pacjentów...
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Identification of the Contamination Source Location in the Drinking Water Distribution System Based on the Neural Network Classifier
PublicationThe contamination ingression to the Water Distribution System (WDS) may have a major impact on the drinking water consumers health. In the case of the WDS contamination the data from the water quality sensors may be efficiently used for the appropriate disaster management. In this paper the methodology based on the Learning Vector Quantization (LVQ) neural network classifier for the identification of the contamination source location...
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Trace Metal Contamination of Bottom Sediments: A Review of Assessment Measures and Geochemical Background Determination Methods
PublicationThis paper provides an overview of different methods of assessing the trace metal (TM) contamination status of sediments affected by anthropogenic interference. The geochemical background determination methods are also described. A total of 25 papers covering rivers, lakes, and retention tanks sediments in areas subjected to anthropogenic pressure from the last three years (2019, 2020, and 2021) were analysed to support our examination...
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Zanieczyszczenie wód gruntowych odciekami ze składowiska odpadów. Groundwater contamination by the leakeage from the landfill.
PublicationJednym z poważnych zagrożeń środowiska są stare składowiska odpadów z uwagi na ich nieodpowiednią lokalizację bądź zabezpieczenia nie spełniające obecnie obowiązujących wymagań i unormowań prawnych. Sytuację taką zaobserwowano na analizowanym obszarze badawczym. Zalegające na tym terenie odpady nie są izolowane od warstwy wodonośnej, w związku z czym wody opadowe filtrujące przez warstwę odpadów powodują zanieczyszczenie wód gruntowych...
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Contamination of runoff waters from roads with high traffic intensity in the urban region of Gdańsk, Poland
PublicationW ramach pracy prowadzone były badania zanieczyszczeń w próbkach wód spływnych z arterii komunikacyjnych. Próbki pobierano z sześciu stałych punktów zlokalizowanych wzdłuż szlaku komunikacyjnego prowadzącego z Redy do Gdańska (z miejsc o dużym natężeniu ruchu pojazdów) oraz z ulicy M. Buczka w Redzie (o mniejszym natężeniu ruchu). Próbki pobierane były przez okres dwóch miesięcy (okres deszczów jesiennych: 24.10 - 18.12.2000) oraz...
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Analysis of the influence of ferromagnetic contaminations in plastics on the measurements of disturbances of magnetic field of the Earth
PublicationPlastics can be contaminated with small particles or filings with ferromagnetic properties. These contaminations cause significant disturbances in the magnetic field in the case of taking measurements of very low magnetic fields. The analysis of the influence of contaminated plastics on magnetic measurements is presented in this paper.
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Bio based PUR-PIR foams thermal degradation (TG) and isoconversional kinetics
Open Research DataIn the present work, we perform a thorough thermogravimetric (TG) analysis of the bio-based polyurethane – polyisocyanurate (PUR-PIR) foams in both nitrogen and oxygen atmosphere. A sustainable element of the foam was a biopolyol obtained via acid-catalyzed liquefaction of Zostera Marina and Enteromorpha Algae biomass. Based on isoconversional analysis...
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Influence of bio-polyols with different molecular weight on properites of PUR-PIR foams
Open Research DataThis work attempts to validate the possibility of replacing petrochemical polyols with previously synthesized bio-polyols and their impact on the structure and properties of rigid polyurethane-polyisocyanurate (PUR-PIR). The influence of bio-polyols addition on foam properties was investigated by mechanical testing, Fourier transform infrared spectroscopy...
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A hypothesis for GPCR activation
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ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
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Zakażenia bakteryjne w przewlekłym zapaleniu ucha środkowego - ocena przydatności diagnostyki molekularnej opartej o technikę PCR. Bacterial infection in chronic otitis media - assessment of usefulness of molecular diagnostic based in PCR method.
PublicationIdentyfikacja zakażeń w przewlekłym zapaleniu ucha środkowego jest trudna, o czym świadczą wysokie odsetki ujemnych wyników badań bakteriologicznych. Przyczyną tego jest trudność w uzyskiwaniu wiarygodnego materiału do badań oraz stosunkowo niska czułość klasycznych metod diagnostycznych. Amplifikacja bakteryjnego DNA techniką PCR jest badaniem, które może przyczynić się do zwiększenia efektywności i czułości badań bakteriologicznych....
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DNA Methylation Changes Induced by Redox-Active Compounds—Choosing the Right PCR-Based Method
PublicationThe impact of catechins on the expression profile of redox-related genes in HT29 cell line has been studied recently by our group using Oxidative Stress RT2 Profiler PCR Array. Within the examined panel of 84 genes, the down-regulation of SRXN1 gene was unique among other up-regulated genes. We hypothesized that the observed down-regulation resulted from DNA methylation and have exploited this observation to choose the proper strategy...
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Evaluation of a PCR Melting Profile method for intra-species differentiation of Trichophyton rubrum and Trichophyton interdigitale
PublicationW celu identyfikacji źródła infekcji spowodowanych przez dermatofity, jak i drogi ich transmisji, istnieje potrzeba szukania nowych metod, które pozwolą na głębokie zróżnicowanie genetyczne szczepów w obrębie rodzaju. W pracy po raz pierwszy pokazano zastosowanie techniki PCR MP opartej o adaptory oligonukleotydowe i różnice w temperaturze topnienia fragmentów restrykcyjnych DNA w reakcji PCR do wewnątrzgatunkowego typowania dermatofitów....
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Application of PCR-HRM method for microsatellite polymorphism genotyping in the LDHA gene of pigeons (Columba livia)
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Evaluation of possibilities of genotyping of Candida glabrata clinical isolates with RAPD-PCR method and microsatellite analysis
PublicationThe aim of the study was to compare the discriminatory power of RAPD-PCR method using RSD10 primer and microsatellite (GACA)4 analysis for genotyping clinical isolates of C. glabrata. Isolates were received from patients of two Polish hospitals: Children's Memorial Health Institute in Warsaw (n=17) and Medical University of Gdansk (n=37). Species identification was confirmed by two phenotypic methods: growth on chromagar plates...
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System do diagnostyki dermatofitowych zakażeń powierzchniowych oparty na technice Real-Time PCR
PublicationDermatofity należą do blisko spokrewnionej grupy grzybów, które wykazują wysokie powinowactwo do skeratynizowanych tkanek. Cecha ta czyni je odpowiedzialnymi za powierzchniowe grzybice skóry, paznokci oraz włosów. Szacuje się, iż nawet do 20% ludzi na całym świecie dotkniętych jest infekcjami powodowanymi przez dermatofity. Ich leczenie wymaga długotrwałego zastosowania leków przeciwgrzybiczych. W celu dobrania odpowiedniego leczenia...
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New PCR test for detection of Candida glabrata based on the molecular target chosen by the RAPD technique
PublicationRapid, reliable diagnosis is a necessary condition for the successful treatment of infections. Such diagnostic assays are continually being developed. #e paper presents a method for selecting the molecular target for PCR-based diagnostics based on the comparison of RAPD patterns. A sequence encoding Candida glabrata CBS138 hypothetical protein was selected. The limit of detection for PCR and real-time PCR reactions with DNA extracted...
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The use of a one-step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets
PublicationIntroduction: Dermatophytes are a closely related group of keratinophilic fungi. They encompass important etiological agents of superficial fungal infections. These fungi are able to invade keratinized tissues of humans and animals, causing dermatophytosis (ringworm) of hair, nails or skin. The aim: Traditional diagnostics of ringworm is based on morphological identification of cultured fungi and is time-consuming. Materials and methods:...
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Usefulness of the PCR technique for bacterial DNA detection in blood of the patients after ''oppened heart'' operations.
PublicationOperacje kardiochirurgiczne w krążeniu pozaustrojowym (KPU) poprzez ogromny uraz, powodowanie występowania zaburzeń odporności oraz uszkodzenie barier anatomicznych stwarzają sytuację wybitnie sprzyjającą rozwojowi powikłań infekcyjnych u tych chorych. Prowadzenie terapii antybiotykowej profilaktycznej i leczniczej, a także selektywna dekontaminacja przewodu pokarmowego może utrudniać i przedłużać czas rozpoznania obecności bakterii...
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Biological responses to environmental contamination. How can metal pollution impact signal honesty in avian species?
PublicationEnvironmental pollution, for example with metals, can significantly affect the ecosystem balance leading to severe changes. Biologically active pigments are relevant for the appearance and condition of birds. Melanin and carotenoid particles are the most frequently deposited pigments in avian integument. They are responsible for the majority of colors of bird plumage. The phenotypic expression can be affected by metal contamination....
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Contamination of Runoff Water at Gdańsk Airport (Poland) by Polycyclic Aromatic Hydrocarbons (PAHs) and Polychlorinated Biphenyls (PCBs)
PublicationAirport runoff can contain high concentrations of various pollutants, in particular polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs), the environmental levels of which have to be monitored. Airport runoff water samples, collected at the Gdańsk-Rębiechowo Airport from 2008 to 2009, were analysed for PAHs and PCBs by gas chromatography. The aromatic fractions were separated by liquid-liquid extraction...
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Identification of the featured-element in fine road dust of cities with coal contamination by geochemical investigation and isotopic monitoring
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