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Phosphorylation‐mediated regulation of heat shock response in Escherichia coli
PublikacjaEscherichia coli has two heat shock regulons under the transcriptional control of Esigma(32) and Esigma(E) RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that mutations in a tyrosine phosphatase-encoding gene led to decreased activity of these heat shock regulons. The...
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An IoT-Based Computational Framework for Healthcare Monitoring in Mobile Environments
PublikacjaThe new Internet of Things paradigm allows for small devices with sensing, processing and communication capabilities to be designed, which enable the development of sensors, embedded devices and other ‘things’ ready to understand the environment. In this paper, a distributed framework based on the internet of things paradigm is proposed for monitoring human biomedical signals in activities involving physical exertion. The main...
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Potential energy curves, transition and permanent dipole moments of KRb
PublikacjaWe present extensive calculations of 48 adiabatic potential energy curves of the KRb molecule. Efforts have been focused on preparing the appropriate basis sets. Compared to previous approaches, the set of new potential energy curves is extended to higher excitations, including the single-excited K(4s2S)+Rb(5d2D) and double-excited K(4p2P)+Rb(5p2P) atomic limits. Larger distances between nuclei are also taken into account. New...
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Sensing the onset of epoxy coating degradation with combined Raman spectroscopy/atomic force microscopy/electrochemical impedance spectroscopy
PublikacjaThe paper presents the results of investigation on epoxy resin durability upon 12-week exposure to UV radiation. The aim was early determination of the onset of epoxy degradation and for this purpose an epoxy film on steel substrate systems were periodically inspected using Raman spectroscopy, atomic force microscopy and electrochemical impedance spectroscopy. The behaviour of examined polymer could be divided into three periods: immunity,...
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Micro-modelling of shear localization during quasi-static confined granular flow in silos using DEM
PublikacjaThe paper deals with the quasi-static confined flow of cohesionless sand in a plane strain model silo with parallel walls and a slowly movable bottom. Numerical modelling was carried out by the discrete element method (DEM) using spheres with contact moments to approximately capture a non-uniform shape of sand particles. Different initial void ratios of sand and silo wall roughness grades were employed. Regular triangular grooves...
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EXPERIMENTAL AND NUMERICAL INVESTIGATIONS OF CONCRETE BEHAVIOUR AT MESO-LEVEL DURING QUASI-STATIC SPLITTING TENSION
PublikacjaThe paper describes experimental and numerical results of quasi-static splitting tensile tests on concrete specimens at meso-scale level. The loading strip was made of plywood or steel. Fracture in concrete was detected at the aggregate level by means of three non-destructive methods: 3D x-ray micro-computed tomography, 2D scanning electron microscope and manual 2D digital microscope. The discrete element method was used to directly...
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Identification of the Contamination Source Location in the Drinking Water Distribution System Based on the Neural Network Classifier
PublikacjaThe contamination ingression to the Water Distribution System (WDS) may have a major impact on the drinking water consumers health. In the case of the WDS contamination the data from the water quality sensors may be efficiently used for the appropriate disaster management. In this paper the methodology based on the Learning Vector Quantization (LVQ) neural network classifier for the identification of the contamination source location...
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Comparison of Different Measurement Techniques as Methodology for Surveying and Monitoring Stainless Steel Chimneys
PublikacjaThe measurement of a chimney axis is an engineering geodesy task. These measurements allow to assess the technical condition of chimneys and are usually carried out for reinforced concrete chimneys. When measuring high chimneys, the main problem may be the need to go a long distance to take a measurement. The surface of the chimney can also be problematic because it can absorb so much light from the range finder that total station...
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Investigation of the 16-year and 18-year ZTD Time Series Derived from GPS Data Processing
PublikacjaThe GPS system can play an important role in activities related to the monitoring of climate. Long time series, coherent strategy, and very high quality of tropospheric parameter Zenith Tropospheric Delay (ZTD) estimated on the basis of GPS data analysis allows to investigate its usefulness for climate research as a direct GPS product. This paper presents results of analysis of 16-year time series derived from EUREF Permanent Network...
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Chapter 8 – Active Sampling of Air
PublikacjaThis chapter reviews the literature information on analytical techniques and laboratory equipment used for active sampling of air (atmospheric and indoor) in regular monitoring research. It describes popular analytical devices applied for sample collection using various types of polymeric bags, e.g. Tedlar bags, Teflon or Nafion bags, and/or stainless steel vacuum containers. It reviews literature data about the application of...
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Nowoczesne metody termochemicznej konwersji biomasy w paliwa gazowe, ciekłe i stałe = Modern methods of thermochemical biomass conversion into gas, liquid and solid fuels
PublikacjaBiomass utilization through direct- or co-combustion with coal, based on coal, hydrogen and oxygen compounds chemical energy conversion into heat in boilers, is simultaneously the cheapest and - according to experts - economically least effective solution. In case of heat and electricity production in cogeneration process in biomass fueled heat and power stations (wood, straw, energetic plants, RDF etc), investment costs are little...
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Fuel price, income and road safety as determinants of the level of the population’s economic well-being in Poland
PublikacjaThe opportunity to travel is one of the most favorite human activities, given that on a trip a person gets new knowledge, impressions and positive emotions. Recreational trips occupy a prominent place in the concept of the economics of happiness, and the study of factors that influence decision-making regarding travel is important for forecasting the number of tourists, infrastructure development, income and expenses of businesses...
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Ontologie vs. reguły — porównanie metod reprezentacji wiedzy na przykładzie dziedziny zarządzania usługami informatycznymi
PublikacjaTekst stanowi krótki przegląd wybranych konstrukcji służących reprezentacji wiedzy w postaci ontologii opartych na logice opisowej i porównanie ich z odpowiednikami opartymi na zapisie regułowym. Z powodu ograniczonej liczby stron pokazano tylko elementy związane z reprezentacją konceptów, bez uwzględniania ról. Do zapisu ontologii wykorzystano formalizmy języka OWL, zaś reguły wyrażono w Prologu. Dla lepszego zilustrowania tych...
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Analityczna metoda projektowania łuków odwrotnych
PublikacjaW pracy przedstawiono nową metodę projektowania rejonu zmiany kierunku trasy kolejowej, dostosowaną do techniki Mobilnych Pomiarów Satelitarnych. Metoda ta może się okazać szczególnie przydatna wówczas, gdy obydwu prostych kierunków trasy nie można połączyć w sposób elementarny, stosując łuk kołowy z krzywymi przejściowymi; dotyczy to również. zastosowania łuku koszowego. Jedynym rozwiązaniem staje się wówczas wprowadzenie do układu...
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Influence of the ionic strength on the amyloid fibrillogenesis of hen egg white lysozyme
PublikacjaThe study investigates the role of the electrostatic interactions in the fibrillation of the hen egg white lysozyme (HEWL). In order to achieve this aim the influence of the cations Na+, Mg2+ and Al3+ on the amyloid fibril formation and amorphous aggregation was tested. The amyloids are formed in the solution without added salt but the Thioflavin T fluorescence gives the false-negative result. In these conditions, the HEWL fibrils...
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Redox property switching in MOFs with open metal sites for improved catalytic hydrogenation performance
PublikacjaIn this study, a decompression-thermalization strategy was utilized for a Cu-containing metal-organic framework (MOF) to tune its redox and catalytic hydrogenation properties. Remarkably, at just 120 °C, open metal sites (OMSs) were successfully constructed by removing coordinated solvent molecules to generate the daughter MOF-120. The tailored redox property of MOF-120 is correlated with the presence of OMSs. Compared with the...
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Effectiveness of toluene separation from gas phase using supported ammonium ionic liquid membrane
PublikacjaAmmonium ionic liquids (ILs) are relatively cheap in synthesis and environmentally benign and despite that they have been very rarely used in gas separation. In this research we used several ammonium ILs as liquid membranes for removal of residual toluene from gas phase. Ionic liquids used in this study were composed of bis(trifluoromethylsulfonyl)imide anion [Tf2N] and trimethylbutylammonium [N1114], trietylbutylammonium [N2224],...
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Synthesis, structural and spectroscopic properties of asymmetric schiff bases derived from 2,3-diaminopyridine
PublikacjaTwo Schiff base derivatives, 4-(2-amino-3-pyridyliminomethyl)phenol (I) and 3-(2-amino-3- pyridyliminomethyl)nitrobenzene (II ), were synthesised and characterised by spectroscopy. The structure of I was determined by single crystal X-ray diffraction studies. The asymmetric Schiff base derived from 2,3-diaminopyridine selectively recognise transition and heavy metal cations, and some anion. Ligands I and II form stable complexes...
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Contaminants in airport runoff water in the Vicinities of Two International Airports in Poland
PublikacjaThe general aim of the study was to evaluate environmental quality associated with the operations (combustion of fuels, cleaning of aircraft and airport aprons, aircraft, ground vehicle maintenance and repairs, fuelling operations, de/anti-icing operations) of international airports located in central and northern of Poland. These two monitoring areas were set up to monitor PAHs, PCBs, formaldehydes, phenols, detergents (cationic,...
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Enhanced Electrochemical Performance of MnCo1.5Fe0.5O4Spinel for Oxygen Evolution Reaction through Heat Treatment
PublikacjaMnCo1.5Fe0.5O4 spinel oxide was synthesized using the sol−gel technique, followed by heat treatment at various temperatures (400, 600, 800, and 1000 °C). The prepared materials were examined as anode electrocatalysts for watersplitting systems in alkaline environments. Solid-state characterization methods, such as powder X-ray diffraction and X-ray absorption spectroscopy (XAS), were used to analyze the materials’ crystallographic...
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Has EGNOS its own place in maritime navigation?
PublikacjaGNSS (in fact it usually means GPS) is at the moment the fundamental means of positioning for any variants of navigation. However some attributes of it can be not sufficient for every users or in some tasks, so there are any cases when this system needs support. From the mariner point of view pure GPS properties perfectly fulfils expectations of sailors at the open sea, however there are lot of cases, when accuracy of the system...
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General solution of quantum mechanical equations of motion with time-dependent Hamiltonians: A Lie algebraic approach
PublikacjaThe unitary operators U(t), describing the quantum time evolution of systems with a time-dependent Hamiltonian, can be constructed in an explicit manner using the method of time-dependent invariants. We clarify the role of Lie-algebraic techniques in this context and elaborate the theory for SU(2) and SU(1,1). In these cases we give explicit formulae for obtaining general solutions from special ones. We show that the constructions...
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Evolutionary Sets of Cooperating Ship Trajectories: COLREGS Compliance
PublikacjaThe paper presents a newly designed improvement to the method of solving multi-ship encounter situations. In general, the method combines some of the assumptions of game theory with evolutionary programming and aims to find optimal set of cooperating trajectories of all ships involved in an encounter situation. The improvement presented here is a new way of modelling some of the COLREGS rules. Due to this change, the method is...
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On weak solutions of the boundary value problem within linear dilatational strain gradient elasticity for polyhedral Lipschitz domains
PublikacjaWe provide the proof of an existence and uniqueness theorem for weak solutions of the equilibrium problem in linear dilatational strain gradient elasticity for bodies occupying, in the reference configuration, Lipschitz domains with edges. The considered elastic model belongs to the class of so-called incomplete strain gradient continua whose potential energy density depends quadratically on linear strains and on the gradient of...
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Two-Row ESPAR Antenna with Simple Elevation and Azimuth Beam Switching
PublikacjaIn this letter, we propose a two-row electronically steerable parasitic array radiator (ESPAR) antenna designed for direction of arrival (DoA) estimation in Internet of Things (IoT) applications relying on simple microcontrollers. The antenna is capable of elevation and azimuth beam switching using a simple microcontroller-oriented steering circuit and provides 18 directional radiation patterns, which can be grouped in 3 distinctive...
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Secure Quaternion Feistel Cipher for DICOM Images
PublikacjaAn improved and extended version of a quaternion-based lossless encryption technique for digital image and communication on medicine (DICOM) images is proposed. We highlight and address several security flaws present in the previous version of the algorithm originally proposed by Dzwonkowski et al. (2015). The newly proposed secure quater- nion Feistel cipher (S-QFC) algorithm...
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Identification of safety hazards and their sources in tram transport
PublikacjaBoth in Poland and in most countries of the European Union, the tram infrastructure is extensively expanding. Contemporary low-floor trams are constructed according to different technical assumptions than typical railway vehicles or high floor trams. In Poland such vehicles rides often on lines designed according to standards developed to the classical vehicles. At the same time, the intensity of road traffic and the number of...
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Hydrodynamic Model of the New Waterway through the Vistula Spit
PublikacjaThe decision to build a new waterway (strait) in the Polish part of the Vistula Spit was made in 2017. The new connection between the Gulf of Gdańsk and the Vistula Lagoon is planned as an artificial navigable channel with a lock and a small port. During storm surges and wind tides in the gulf or in the lagoon, sluicing will be re-quired for vessels to tackle the Vistula Spit. This procedure does not require significant water flow...
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Nonlinear finite element modeling of vibration control of plane rod-type structural members with integrated piezoelectric patches
PublikacjaThis paper addresses modeling and finite element analysis of the transient large-amplitude vibration response of thin rod-type structures (e.g., plane curved beams, arches, ring shells) and its control by integrated piezoelectric layers. A geometrically nonlinear finite beam element for the analysis of piezolaminated structures is developed that is based on the Bernoulli hypothesis and the assumptions of small strains and finite...
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Theoretical Investigation of the Electron-Transfer Dynamics and Photodegradation Pathways in a Hydrogen-Evolving Ruthenium-Palladium Photocatalyst
PublikacjaTime-dependent density functional theory calcula- tions combinedwith the Marcus theory of electron transfer (ET) were app lied on the molecular photocatalys t [(tbbpy) 2 Ru(tpph z)PdC l 2 ] 2 + in order to elucidate thelight-in- duced relaxation pathways populated upon excitation in the longer wavelength range of its absorption spectrum. The computational resultsshow that after the initial excitation, metal (Ru) to ligand (tpphz)chargetransfer...
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Apprendere camminando.Il metodo della Jane’s Walk per riscoprire San Lorenzo
PublikacjaTratta dal libro rivoluzionario che Jane Jacobs, attivista americana, ha scritto negli anni Sessanta e che ben presto è diventato un importante riferimento per la partecipazione dei cittadini nella progettazione urbana, questa citazione ben esprime l’approccio attraverso il quale ci siamo avvicinate alla riscoperta di San Lorenzo. Quartiere della città storica molto conosciuto, San Lorenzo si è rivelato in grado di disvelare paesaggi...
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Increasing the Seismic Resistance of Wood-frame Buildings by Applying PU Foam as Thermal Insulation
PublikacjaWood-frame buildings are very common in regions that are exposed to earthquakes. Most of residential buildings are constructed using this technology; therefore, the seismic resistance of them is really essential in order to prevent human losses and structural damage. The aim of the present article is to show the results of the detailed numerical FEM analysis focused on the seismic behaviour of the wood-frame house with different...
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The impact of methods the stochastic analysis on swimming safety of multihull floating units (Part1)
PublikacjaThe presented article concerns the application of the methods of the stochastic analysis to solve differential equations for multihull catamaran-type floating unit. There was described the continuous process of Markov and the method of equations of Focker-Planck-Kolmogorov. The analysis of dynamics of the multihull unit was carried out with the assumption that the system model is the linear model with six degrees of freedom, on...
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Real-time simulator of agricultural biogas plant
PublikacjaThis article presents a real-time simulator of an agricultural biogas plant. The project contains biogas and biomass circuits simulation, as well as heating circuit simulation with a complete control system and visualization interface of the whole process. The software tool used to simulate the plant work is CFD (Computational Fluid Dynamics), which enables a user to create and test simulation objects based on fundamental physical...
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Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage
PublikacjaExposure to high levels of different environmental pollutants is known to be associated with induction of DNA damage in humans. Thus DNA repair is of great importance in preventing mutations and contributes crucially to the prevention of cancer. In our study we have focused on quantitative analysis of Gentiana asclepiadea aqueous or methanolic extracts obtained from flower and haulm, their antioxidant potency in ABTS post-column...
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Controlled Random Search Applied to Parameters Estimation of the Longitudinal Solutes Transport Model for Rivers
PublikacjaNumerical computations are presented for the longitudinal transport of passive, conservative solutes in an actual river with the inclusion of geometrical complexities of river channels. A special emphasis is put on the method of the identification of model parameters which is based on a specially designed optimisation procedure using random control search algorithm. Two different situations are considered namely a linear version...
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Investigation on Damage-Involved Structural Response of Colliding Steel Structures during Ground Motions
PublikacjaEarthquakes are the most unpredictable damaging loads which can affect civil engineering structures. Due to insufficient separation distance between adjacent structures with different dynamic properties, structural collisions may occur during ground motions. Although the research on structural pounding has recently been much advanced, the studies have mainly been conducted for concrete structures. The aim of this paper is to show...
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Organizational culture as prerequisite of proactive safety and security management in critical infrastructure systems including hazardous plants and ports
PublikacjaThis article addresses selected aspects of organizational culture to be considered in the context of knowledge based proactive safety and security management of plants, ports and systems of critical infrastructure. It has been often emphasized in the domain literature that business effectiveness of such plants and their resilience against hazards and threats to avoid major accidents depends substantially on human and organizational...
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A method of determining and visualizing safe motion parameters of a ship navigating in restricted waters
PublikacjaThe paper presents a method of displaying ship collision avoidance information which is based on an unconventional Collision Threat Parameters Area (CTPA) technique. The solution presented here extends CTPA's functionality from past works by supporting navigation in restricted waters and handling ship domains analytically instead of numerically. It visualizes potential navigational threats as well as possible collision avoidance...
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Principles of target DNA cleavage and the role of Mg2+ in the catalysis of CRISPR–Cas9
PublikacjaAt the core of the CRISPR–Cas9 genome-editing technology, the endonuclease Cas9 introduces site-specific breaks in DNA. However, precise mechanistic information to ameliorate Cas9 function is still missing. Here, multimicrosecond molecular dynamics, free energy and multiscale simulations are combined with solution NMR and DNA cleavage experiments to resolve the catalytic mechanism of target DNA cleavage. We show that the conformation...
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Low-Barrier Hydrogen Bond Determines Target-Binding Affinity and Specificity of the Antitubercular Drug Bedaquiline
PublikacjaThe role of short strong hydrogen bonds (SSHB) in ligand-target binding remains largely unexplored, thereby hin- dering a potentially important avenue in the rational drug de- sign. Here, we investigate the interaction between bedaquiline (Bq), a potent anti-tuberculosis drug, and the mycobacterial ATP synthase, to unravel the role of a specific hydrogen bond to a conserved acidic residue in the target affinity and specificity....
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WSPÓŁCZESNE OCHŁODZENIE REJONU SZETLANDÓW POŁUDNIOWYCH I PÓŁNOCO-ZACHODNICH KRAŃCÓW PÓŁWYSPU ANTARKTYCZNEGO
PublikacjaThe paper discusses changes of hydro-climatic conditions occurring in the South Shetland Islands and in NW parts of the Antarctic Peninsula in the 1979–2016 years period. These changes are important for evaluation of the transformation of marine and coastal ecosystems. The general feature of these changes was a transition from the strong warming phase to the cooling phase, which took place around the year 2000. The paper presents...
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ANALYSIS OF CLIMATIC ZONES IN POLAND WITH REGARD TO ASPHALT PERFORMANCE GRADING
PublikacjaTowards the end of the last century a new system of bitumen grading was developed and implemented as part of the American Strategic Highway Research Program (SHRP). Its aim was to better adjust the testing methods and requirements for road bitumens to the actual conditions in which those bitumens serve in road pavements. Currently in Poland, similarly as in the other EU countries, road bitumens are tested and classified with regard...
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Determination of benzo(a)pyrene content in PM10 using regression methods
PublikacjaThe paper presents an attempt of application of multidimensional linear regression to estimation of an empirical model describing the factors influencing on B(a)P content in suspended dust PM10 in Olsztyn and Elbląg city regions between 2010 and 2013. During this period annual average concentration of B(a)P in PM10 exceeded the admissible level 1.5-3 times. Conducted investigations confirm that the reasons of B(a)P concentration...
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THE APPLICATION OF KALMAN FILTERING TO PREDICT VERTICAL RAIL AXIS DISPLACEMENTS OF THE OVERHEAD CRANE BEING A COMPONENT OF SEAPORT TRANSPORT STRUCTURE
PublikacjaAs the devices designed to transport materials, the overhead cranes should meet certain geometric requirements for their operation to be safe. The presently available geodetic equipment, in particular total stations, provides opportunities for precise 3D measurements of coordinates of the controlled points. These coordinates make a basis for correcting the height of crane runway axes. The paper presents a method to calculate position...
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Distributed Framework for Visual Event Detection in Parking Lot Area
PublikacjaThe paper presents the framework for automatic detection of various events occurring in a parking lot basing on multiple camera video analysis. The framework is massively distributed, both in the logical and physical sense. It consists of several entities called node stations that use XMPP protocol for internal communication and SRTP protocol with Jingle extension for video streaming. Recognized events include detecting parking...
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Experimental test results of an automatic voltage regulator with independent phase voltage controllers
PublikacjaThe growing number of distributed renewable energy sources and dynamic constant-power loads (e.g. electric vehicle charging stations) pose new challenges for network operators. These changes result in alterations to network load profiles and load flows, leading to greater voltage volatility. One effective solution to these problems can be the use of automatic voltage regulators (AVRs), which stabilize and symmetrize voltage output,...
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The novel sterilization device: the prototype testing
PublikacjaCurrently, there are numerous methods that can be used to neutralize pathogens (i.e., devices, tools, or protective clothing), but the sterilizing agent must be selected so that it does not damage or change the properties of the material to which it is applied. Dry sterilization with hydrogen peroxide gas (VHP) in combination with UV-C radiation is well described and effective method of sterilization. This paper presents the design,...
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The role and scope of architectural and conservation research procedures in the process of restoring the splendour of an architectural monument created after 1945 on the example of the Suburban Railway Station in Gdynia
PublikacjaModern Movement buildings, despite their relatively recent construction, are sometimes affected by radical transformations of layout and form. The railway stations being one of the most important public buildings in the city are a special group of them. Due to long-term and intensive use they may bear the signs of exploitation of parts of its substance and have secondary transformations disharmony with the building. The subject...
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Products of Photo- and Thermochemical Rearrangement of 19-Membered di-tert-Butyl-Azoxybenzocrown
PublikacjaThe preparation and characterization of products of the photochemical and thermochemical rearrangements of 19-membered azoxybenzocrowns with two, bulky, tert-butyl substituents in benzene rings in the para positions to oligooxyethylene fragments (meta positions to azoxy group, i.e., t-Bu-19-Azo-O have been presented. In photochemical rearrangement, two colored typical products were expected, i.e., 19-membered o-hydroxy-m,m′-di-tert-butyl-azobenzocrown...