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The New LM-PCR/Shifter Method for the Genotyping of Microorganism
PublikacjaTechniques relies on the ligation of appropriates adapters (LM-PCR) as AFLP, PCR MP and ADSRRS are successfully used for epidemiological studies for prokaryotic and eukaryotic microorganisms. In this study we propose a new method, called the LM-PCR/Shifter, based on the use of a Class IIS restriction enzyme giving restriction fragments with different 4 base 5' overhangs (Shifter) and the ligation of appropriate oligonucleotide...
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DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation
PublikacjaA new diagnostic kit for K. oxytoca specific differentiation based on ddLMS PCR (ang. double digest ligation Mediated PCR) technique is shown. As a species-specific DNA fragment pehX gene, encoding the enzyme polygalactouronase, was chosen. The genome sequence of K. oxytoca is digested with two endonucleases: AclI and BclI which cut DNA before and after pehX gene. The polymorphic DNA fragments are ligated with AclI-end-specific...
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Evidence-Based Risk Management for Civil Engineering Projects Using Bayesian Belief Networks (BBN)
PublikacjaThe authors are seeking new methods for improving the efficiency of the investments associated with the maintenance and operation of existing civil engineering structures. It is demonstrated how the knowledge about the elements of construction and operation phases and their relationships, combined with monitoring data can be used for more effective management of the risks associated with civil engineering projects. The methodology...
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A Model for Risk Assessment and Management of Construction Projects in Urban Conditions
PublikacjaThe authors are seeking new methods for improving the efficiency of the investments associated with the maintenance and operation of existing civil engineering structures. It is demonstrated how the knowledge about the elements of construction and operation phases and their relationships, combined with monitoring data can be used for more effective management of the risks associated with civil engineering projects. The methodology...
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The influence of historical conditions on time and cost of construction project
PublikacjaConducting construction works in a building or in an area entered in the historic register is associated with the contracting authority's risk of incurring higher than planned costs of works, as well as delays in their implementation. In many cases, these deviations are completely independent of contracting authority and contractor. The aim of this article is to indicate the reasons for cost and time changes in the construction...
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Building Information Modelling as an opportunity and risk for stakeholders involved in construction investment process
PublikacjaThe requirements to apply Building Information Modelling (BIM) in public investments worldwide are currently very high. Significant interest (sometimes formulated also as a requirement) in BIM technology can be observed also among private investors. Design technology that applies BIM is supported by many private investors due to its numerous advantages. A growing group of construction designers (steel, concrete and reinforced concrete...
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Modyfikacje polimeraz DNA jako rozwiązania problemów w reakcjach PCR
PublikacjaReakcja PCR już od wielu lat jest podstawowym narzędziem stosowanym w biotechnologii molekularnej. Nadal napotykamy jednak liczne problemy podczas jej przeprowadzania związane z trudnymi matrycami, obecnością inhibitorów itp. Optymalizacja reakcji PCR jest więc niezbędna w celu poprawy wyników. Jednym z rozwiązań tych problemów może być modyfikowanie polimeraz DNA. W powyższym artykule opisano problemy i rozwiązania zależne polimerazy...
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Application of Bayesian Networks in risk diagnostics arising from the degree of urban regeneration area degradation
PublikacjaUrban regeneration as a complex project, generates many extremely specific threats affecting the increase of investment risk. Its unique nature causes that probability parameter, normally applied in the process of risk quantification, is extremely difficult to estimate. Due to lack of historical data urban regeneration related activities are therefore associated with uncertainty. According to the authors, a useful tool for resolving...
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Application of BN in Risk Diagnostics Arising from the Degree of Urban Regeneration Area Degradation
PublikacjaUrban regeneration as a complex project, generates many extremely specific threats affecting the increase of investment risk. Its unique nature causes that probability parameter, normally applied in the process of risk quantification, is extremely difficult to estimate. Due to lack of historical data urban regeneration related activities are therefore associated with uncertainty. According to the authors, a useful tool for resolving...
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The life cycle cost of a building from the point of view of environmental criteria of selecting the most beneficial offer in the area of competitive tendering
PublikacjaThe article analyses environmental and ecological criteria of selecting the most beneficial offer in the aspect of LCC. Construction works contracts and the potential method of defining the above criteria, among others, is pondered on (for example by the recommendation of a material, which is supposed to be used, a ban on substances that are harmful for human health as well for the environment). In the relation to the above, it...
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Building Information Modeling in Small and Middle Sized Buildings – Case Study
PublikacjaBuilding Information Modeling (BIM) is a technology designed to improve and integrate all engineering aspects in the construction design process. Tools that incorporate the idea of BIM are becoming more advanced and also more popular. Available software solutions have already reached an assumable high level of development, but still are not as widespread and used on nearly as many construction projects as could be expected. Therefore...
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Criteria for evaluation and selection of the best offer for the Contract Engineer service
PublikacjaThe purpose of the legal regulations regarding public procurement in EU countries is to ensure effective funds' spending. When assessing and selecting the best offer, the contracting entities have at their disposal many different criteria, including non-price criteria. Their proper selection and application is necessary to ensure the high quality of the ordered product, delivery or service. Making an order for intellectual services,...
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The Multiplatform Environment for Simulation and Features Estimation of Mixed-Signal Devices
PublikacjaThe use of simulation laboratories is gaining popularity in thedomains of engineering programs. However, the experience in teaching showsthat the simulation itself is not very effective in didactic processes. Teachingprocesses in thefield of specialist subjects, designed for students of technicaluniversities, should be based on direct operations performed by the student onreal devices. At the same time, at the later stages of didactic...
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Wpływ metody trawienia i spiekania na właściwości mechaniczne powłok z nanorurek węglowych osadzonych elektroforetycznie na podłożu Ti13Nb13Zr
PublikacjaDo eksperymentu użyto stopu tytanu Ti13Zr13Nb, który ze względu na swój skład chemiczny i właściwości mechaniczne stanowi doskonały materiał do zastosowań w inżynierii medycznej. Celem pracy była ocena wpływu przygotowania podłoża (rodzaj zastosowanego odczynnika trawiącego) oraz obróbki końcowej (proces spiekania) powłok z wielościennych nanorurek węglowych na ich właściwości mechaniczne (nanotwardość, moduł Younga). Do wytworzenia...
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Cost Analysis of Prefabricated Elements of the Ordinary and Lightweight Concrete Walls in Residential Construction
PublikacjaGlobal economic growth causes an increase in natural resources exploitation, particularly in construction branch. The growing use of electricity contributes to climate change. Therefore, it is necessary to search the solutions, which will allow for reducing natural resources exploitation. One of the many opportunities to do that is the application of the recycled materials. The authors of the given article have analyzed three variants...
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Influence of Property Division on the Technical Condition of a Building
PublikacjaIn this article, the correlation between a technical condition of a building and property division is analyzed and assessed. In the course of the usage of a building, the process of its degradation and the increase in the demands of property occupants can be observed. There is a direct connection between the difficult technical condition of a building and the fragmentation of the ownership structure. Although not considered in...
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Analysis and Risk Evaluation on the Case of Alteration, Revitalization and Conversion of a Historic Building in Gdańsk
PublikacjaEach investment plan, including the one concerning a building, is exposed to the consequences of various types of threats taking place. Therefore, in the case of some large-scale, atypical and complicated building ventures, some actions included in the procedure of risk management should be taken. This will allow for the risk to be eliminated or limited. While preparing a building venture, an investor does not possess full information...
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Spatial pattern of ASG-EUPOS sites
PublikacjaThe article presents the spatial pattern analysis of the ASG-EUPOS permanent GNSS stations in Poland. Using different methods and tools (nearest neighbour, Riplay’s K-function, morphology of Thiessen polygons) we proved that the station distribution model changes within scales. At short distances up to 65 km, which are typical lengths in the network, stations are irregularly dispersed. Increasing this distance to 130 km and over...
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Wykorzystanie fourierowskiej spektroskopii w podczerwieni do badania stężenia gazów wylotowych z tlenkowego ogniwa paliwowego zasilanego biogazem
PublikacjaW dzisiejszych czasach bardzo ważne jest pozyskiwanie energii ze źródeł odnawialnych i bezpiecznych dla środowiska. Jedną z obiecujących technologii są tlenkowe ogniwa paliwowe (ang. Solid Oxide Fuel Cell, SOFC). Stanowią one bezpieczną i ekologiczną alternatywę dla energii uzyskanej z paliw kopalnych. SOFC charakteryzuje się sprawnością energetyczną na poziomie 45-60 %, podczas gdy sprawność klasycznych elektrowni cieplnych nie...
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Effect of osmolytes on the thermal stability of proteins: replica exchange simulations of Trp-cage in urea and betaine solutions
PublikacjaAlthough osmolytes are known to modulate the folding equilibrium, the molecular mechanism of their effect on thermal denaturation of proteins is still poorly understood. Here, we simulated the thermal denaturation of a small model protein (Trp-cage) in the presence of denaturing (urea) and stabilizing (betaine) osmolytes, using the all-atom replica exchange molecular dynamics simulations. We found that urea destabilizes Trp-cage...
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Fizyka skoków narciarskich
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Influence of spatial structures of 316L stainless steel on its cavitation erosion resistance
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Entropy measures of heart rate variability for short ECG datasets in patients with congestive heart failure
PublikacjaWe investigated the usefulness of entropy measures calculated for short ECG series in distinguishing healthy subjects from patients with congestive heart failure (CHF). Four entropy measures were tested: Approximate Entropy (ApEn), Sample Entropy (SampEn), Fuzzy Entropy (FuzzyEn) and Permutation Entropy (PE), each computed for ECG series of 1000, 500, 250 and 100 RR intervals. We found that with a reduction of the data set length...
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Percepcja wiarygodności internetowych serwisów handlowych w kontekście zachowań konsumenckich
PublikacjaCelem pracy było sprawdzenie, w jaki sposób zachowania konsumenckie determinowane czynnikami społecznymi różnicują percepcję atrybutów kształtujących wiarygodność internetowych serwisów handlowych. Doświadczenie w transakcjach on-line oraz poczucie własnej skuteczności konsumentów nie przyczyniały się do powstawanie różnic. Kobiety, inaczej niż mężczyźni, potrzebowały więcej rekomendacji i były bardziej wyczulone na nieprofesjonalizm...
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Comparision of mechanical properties of nitrided cases and remelted layers of austenitic stainless steel
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Corrosion degradation monitoring of ship stiffened plates using guided wave phase velocity and constrained convex optimization method
PublikacjaThe study presents an experimental investigation on the corrosion degradation level assessment using nondestructive wave-based methods. The degradation level of ship structural elements has been assessed in two different ways. The first one is based on the spectral decomposition and zero-crossing incorporated reconstruction of the dispersion curve approach of the antisymmetric Lamb wave mode and the best matching of the theoretical...
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The Laboratory Stand for the Evaluation of Heat Dissipation from the Power Cable in a Casing Pipe
PublikacjaThe current carrying capacity of a power cable determines its efficiency, which depends on its capability to dissipate heat produced inside the cable, as a result of current flow, into the surrounding. The studies were conducted to determine how the characteristics of the medium around the cable affected its temperature and, as a result, its ability to transport electricity. The experimentation in the lab and real measurements...
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Experiment-Based Study of Heat Dissipation from the Power Cable in a Casing Pipe
PublikacjaThe paper deals with the important challenges in terms of electricity transmission by means of underground cable lines. The power cable’s performance is characterized by an ampacity that represents its maximum electric current-carrying capacity. The ampacity of power cables depends on their ability to diffuse the heat generated by the current flow into the environment. In the performed research, the analysis of the efficiency of...
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Experimental and numerical identification of corrosion degradation of ageing structural components
PublikacjaThe study presents experimental and numerical identification of corrosion degradation of thin-walled structural components employing guided wave propagation. The steel structural components are subjected to through-thickness varying corrosion degradation levels. The developed approach using the non-destructive guided wave-propagation quantifies the equivalent average corrosion degradation level by exploring a limited number of...
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Quantitative Risk Assessment in Construction Disputes Based on Machine Learning Tools
PublikacjaA high monetary value of the construction projects is one of the reasons of frequent disputes between a general contractor (GC) and a client. A construction site is a unique, one-time, and single-product factory with many parties involved and dependent on each other. The organizational dependencies and their complexity make any fault or mistake propagate and influence the final result (delays, cost overruns). The constant will...
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Evaluating the antibacterial activity of muramyl dipeptide derivatives, retro-tuftsin derivatives, and anthraquinone oligopeptides against a range of pathogenic bacteria
PublikacjaSearch for new and efficient antibiotic is crucial because of microbial drug resistance and problems with side effects of the administered medication. In this study, we evaluate the in vitro microbiological activity of muramyl dipeptide derivatives, retro-tuftsin derivatives (i.e., tuftsin with reversed amino acid sequences), and combinations of retro-tuftsin derivatives with substituted anthraquinones. The potency of the investigated...
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UN Studio- Nowe trendy w architekturze
PublikacjaCelem recenzji jest prezentacja oraz ocena nowatorskich metod projektowych znanej awangardowej grupy projektowej z Holandii - UN Studio. Analiza monograficznej książki "Move" autorstwa Bena van Berkela i Caroline Bos w kontekście dorobku współczesnej światowej praktyki architektonicznej prowadzi do wniosku, że proponowane przez UN Studio metody projektowe oraz nietuzinkowy pogląd na dziedzinę architektury wnoszą znaczący wkład...
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Usability of UML modeling tools
PublikacjaNarzędzia wspomagające modelowanie w UML pełnią istotną rolę w procesie wytwarzania i utrzymania oprogramowania. Badanie użyteczności tych narzędzi ma na celu poprawę produktywności oraz satysfakcji z ich użytkowania. W referacie zaprezentowano badania użyteczności za pomocą trzech metod: eksperymentalnego testowania efektywności, zapytań o aspekty użyteczności testowanych narzędzi oraz analitycznej metody GOMS (ang. Goals, Operators,...
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Optymalizacja alokacji modułów programistycznych w rozproszonym systemie szkolenia wojskowego
PublikacjaW pracy przedstawiono system metodologiczny do wyznaczania i oceny przydziałów modułów programistycznych w rozproszonym systemie informatycznym, bazującym na systemie MOODLE, wspomagającym zdalne nauczanie i szkolenie wojskowe. Opracowano modele matematyczne rozproszonych systemów komputerowych, na podstawie których sformułowano zadania optymalizacji wielokryterialnej. Główny nacisk położono na zastosowanie algorytmów ewolucyjnych...
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Pantographic metamaterials: an example of mathematically driven design and of its technological challenges
PublikacjaIn this paper, we account for the research efforts that have been started, for some among us, already since 2003, and aimed to the design of a class of exotic architectured, optimized (meta) materials. At the first stage of these efforts, as it often happens, the research was based on the results of mathematical investigations. The problem to be solved was stated as follows: determine the material (micro)structure governed by those...
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Robot-Based Intervention for Children With Autism Spectrum Disorder: A Systematic Literature Review
PublikacjaChildren with autism spectrum disorder (ASD) have deficits in the socio-communicative domain and frequently face severe difficulties in the recognition and expression of emotions. Existing literature suggested that children with ASD benefit from robot-based interventions. However, studies varied considerably in participant characteristics, applied robots, and trained skills. Here, we reviewed robot-based interventions targeting...
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The culture of excellence and its dimensions in higher education
PublikacjaPurpose To recognize the existing state of knowledge on a culture of excellence (CoE) in higher education institutions (HEIs) and to define the CoE in HEI and the dimensions that make up that culture. A subsidiary goal is to propose a qualitative tool to measure CoE maturity. Design/methodology/approach The study was based on the qualitative method, the preferred reporting system of systematic reviews and meta-analysis (PRISMA)....
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Supervised-learning-based development of multi-bit RCS-reduced coding metasurfaces
PublikacjaCoding metasurfaces have been introduced as efficient tools allowing meticulous control over the electromagnetic (EM) scattering. One of their relevant application areas is radar cross section (RCS) reduction, which principally relies on the diffusion of impinging EM waves. Despite its significance, careful control of the scattering properties poses a serious challenge at the level of practical realization. This article is concerned...
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Design of Cost-Efficient Optical Fronthaul for 5G/6G Networks: An Optimization Perspective
PublikacjaCurrently, 5G and the forthcoming 6G mobile communication systems are the most promising cellular generations expected to beat the growing hunger for bandwidth and enable the fully connected world presented by the Internet of Everything (IoE). The cloud radio access network (CRAN) has been proposed as a promising architecture for meeting the needs and goals of 5G/6G (5G and beyond) networks. Nevertheless, the provisioning of cost-efficient...
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Amplification of a single-locus variable-number direct-repeats with restriction fragment length polymorphism (DR-PCR/RFLP) for genetic typing of Acinetobacter baumannii strains
PublikacjaW celu poszukiwania nowych metod typowania genetycznego szczepów Acinetobacter baumannii przydatnych w szpitalnych badaniach epidemiologicznych przeprowadzono analizę dostępnych w banku genów sekwencji szczepów referencyjnych na obecność nowych motywów repetytywnych. Do badania polimorfizmu sekwencji repetytywnych w szczepach Acinetobacter sp. wykorzystano proste powtórzenia typu "direct repeat" (DR) zlokalizowane w jednym miejscu...
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Opracowanie zestawu diagnostycznego do genetycznego typowania szczepów bakteryjnych metodą PCR MP
PublikacjaOmówiono etapy tworzenia zestawu diagnostycznego przeznaczonego do genetycznego typowania szczepów bakteryjnych wykorzystującego procedurę PCR MP (PCR Melting Profiles). Dokonano optymalizacji warunków i walidacji metody w celu przystosowania jej do potrzeb epidemiologicznej oceny zakażeń szpitalnych. Opracowany zestaw stwarza możliwości zastosowania go w laboratoriach prowadzących rutynowo badania diagnostyczne.
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Ocena wrażliwości na fluorochinolony drobnoustrojów izolowanych od pacjentów z zakażeniami układu moczowego leczonych w warunkach ambulatoryjnych
PublikacjaWykazano, że drobnoustroje powodujące zakażenia układu moczowego u pacjentów ambulatoryjnych są nadal wrażliwe na fluorochinolony; dotyczy to jednak drobnoustrojów z rodziny Enterobacteriacae. Natomiast w przypadku zakażeń drobnoustrojami Gram-dodatnimi leki te mogą okazać się nieskuteczne. Stwierdzono silne działanie bakteriobójcze preparatu Betadine na wszystkie badane izolaty. Uzyskano redukcję liczby żywych bakterii w zawiesinie...
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Evaluation of a novel method based on amplification of DNA fragments surrounding rare restriction sites (ADSRRS-figerprinting) for typing strains of vancomycin resistant Enterococcus faecium.
PublikacjaBadania objęły wankomycyno oporne szczepy Enterococcus faecium izolowane od pacjentów oddziału Hematologii Szpitala Klinicznego w Gdańsku. Wykazano, że ADSRRS-fingerprinting jest metodą szybką o dużym potencjale różnicującym i dużej powtarzalności w badaniach epidemiologicznych.
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Evaluation and comparison of random amplification of polymorphic DNA, pulsed field gel electrophoresis and ADSRRS-fingerprinting for typing Serratiamarcescens outbreaks.
PublikacjaADSRRS-fingerprinting, nowa metoda genotypowania oparta na supresji reakcji PCR została zastosowana do badań epidemiologicznych izolatów S. marcescens,pochodzących z trzech różnych epidemii Szpitala Publicznego w Gdańsku. Wyniki uzyskane metodą ADSRRS-fingerprinting porównano z wynikami uzyskanymi technikami RAPD-fingerprinting i PFGE.
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Escherichia coli - zapomniany patogen posocznic
PublikacjaPrzedstawiono próbę retrospektywnej analizy częstości występowania bakteriemii Gram-ujemnych, wywołanych przez E. coli u chorych hospitalizowanych w ośmiu grupach oddziałów klinicznych SPSK1 ACK AM w Gdańsku w latach 2002-2004. Dokonano oceny częstości występowania bakteriemii o etologii E. coli, liczby zgonów związanych bezpośrednio z posocznicą, analizy potencjalnych wrót zakażenia oraz analizy lekowrażliwości szczepów E. coli...
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ANODOWE WARSTWY KATALITYCZNE DLA TLENKOWYCH OGNIW PALIWOWYCH ZASILANYCH BIOGAZEM
PublikacjaW pracy wytworzono materiały (LiLaNi-Al2O3) oraz (Sm0.2Ce0.8O1.9), jako potencjalne katalizatory reakcji reformingu biogazu w tlenkowych ogniwach paliwowych. Syntezę przeprowadzono metodą Pechini’ego. Zbadano skład chemiczny oraz strukturę uzyskanych związków. Ponadto ogniwo sprawdzono podczas pracy z biogazem pod kątem odporności na osadzenie węgla, a także wpływu na długość pracy ogniwa.
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Paradygmat jakościowy w analizie interakcji międzykulturowych – interpretacja na bazie wybranych teorii psychologicznych
PublikacjaIntercultural interactions in a multicultural work environment are a peculiar type of social interactions. The results of prior research on the effects of interactions in such environment are inconclusive. The majority of the previous studies have emphasized problems, applied a quantitative methodology and interpreted the results with regard to social identity and categorization theory, information-processing theory and intergroup contact...
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Intercultural interactions at multinational corporations' workplace: Grounded theory.
Publikacjaenvironments is a new challenge for employees and managers. The aim of the paper is to analyze the social interactions in multicultural environments of multinational corporations (MNCs) as well as to propose a model of intercultural social interactions in MNCs’ specifi c context. Design/methodology/approach: The grounded theory approach was applied to create a model of intercultural interactions in MNCs. The data was obtained during...
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Recurrent bowel-blood translocations of Escherichia coli with the unique virulence characteristics over three-year period in the patient with acute myeloid leukaemia – case report
PublikacjaIn patients with haematological malignancies, the bowel remains the main source of Escherichia coli bloodstream infections. We present the clinical example of recurrent bowel-blood translocations of E. coli with the unique virulence characteristics in a 55-year-old male with the diagnosis of acute myeloid leukaemia. The virulent factors profile of examined strains confirmed that the co-existence of genes papC, sfa, usp and cnf1,...
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Leukemia and risk of recurrent Escherichia coli bacteremia: genotyping implicates E. coli translocation from the colon to the bloodstream.
PublikacjaIn patients with leukemia, the portal(s) and reasons for the persistence of an Escherichia coli recurrent bacteremia remain unclear. Adult Hematology Clinic (AHC) databases at the State Clinical Hospital in Gdańsk were reviewed to evaluate the frequency of E. coli bacteremia between 2002 and 2005. Blood and bowel E. coli strains were obtained and the genetic relatedness of the strains was analyzed. The rate of E. coli bacteremia...