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Search results for: ABIOTIC AND BIOTIC ELICITATIONGC/MS ANALYSISIN VITRO CULTURESINSECT-INDUCED STRESSRITA BIOREACTORTERPENES AND TERPENOIDS
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The role of the enzymatic antioxidant system in cylindrospermopsin-induced toxicity in human lymphocytes
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Earthquake-Induced Structural Pounding
PublicationEarthquake-induced structural pounding between insufficiently separated build-ings, or bridge segments, has been repeatedly observed during ground motions. The reports after earthquakes indicate that it may lead to some local damage in the case of moderate seismic events or may result in considerable destruction or even collapse of colliding structures during severe ground motions. Pounding in build-ings is usually caused by the...
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Rapid Evaluation of Poultry Meat Shelf Life Using PTR-MS
PublicationThe use of proton transfer reaction mass spectrometry (PTR-MS) for freshness classification of chicken and turkey meat samples was investigated. A number of volatile organic compounds (VOCs) were selected based on the correlation (> 95%) of their concentration during storage at 4 °C over a period of 5 days with the results of the microbial analysis. In order to verify if the selected compounds are not sample-specific, a number...
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LC-MS and LC-NMR as Complementary Techniques for the Determination of Pharmaceuticals in Dosage Formulations
PublicationPharmaceuticals contain not only pharmacologically active compounds but also a range of excipients. The danger exists that impurities may also be present in such drugs, which may adversely affect their efficacy and even endanger patient health and life. Monitoring the composition of pharmaceutical products is therefore essential. The trace amounts of such impurities in pharmaceuticals are often identified and determined with liquid...
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Transcriptional activity of epigenetic remodeling genes declines in keratinocytes after in vitro expansion
PublicationPURPOSE: In vitro expansion is an invaluable method to obtain keratinocytes in amounts necessary for effective transplantation therapies. In vitro cell culturing provokes questions concerning potential epigenetic alterations occurring in expanded cells in the context of usefulness for transplantation and safety. The purpose of this study was to investigate as to whether keratinocyte expansion is associated with changes in the activity...
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Problems of PAH quantification by GC-MS method using isotope-labelled standards.
PublicationChromatografia gazowa w połączeniu ze spektromerią mas (GC-MS) jest obecnie rutynowo stosowaną metodą w analityce środowiskowej do oznaczania śladowych ilości zanieczyszczeń organicznych. Stosowanie detektora mas sprawia, że wygodnym wzorcem wewnętrznym są stabilne izotopowo analogi oznaczanych związków. Związki te są wykorzystywane do śledzenia i kompensacji strat analitów podczas wykonywania określonych operacji związanych z...
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Comparative in vitro study of single and four layer graphene oxide nanoflakes — Cytotoxicity and cellular uptake
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Robust HPLC–MS/MS method for levofloxacin and ciprofloxacin determination in human prostate tissue
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Preparation of Internal Standards for 2D-UPLC-MS/MS Quantification of Noncanonical DNA Bases
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Development and Validation of SPE-HPLC-MS/MS Method for Determining Cyclophosphamide in Surface Waters
PublicationA rapid and selective method for trace amounts determination of cyclophosphamide in surface water samples has been developed. A solid phase extraction SPE method for extraction and clean-up procedure has been optimized for determination by reversed-phase high-performance liquid chromatography with tandem mass spectrometry. The analyses proceed in the positive ion mode by means of the electrospray ionization method (ESI). Clean...
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Traffic-induced vibrations. The impact on buildings and people
PublicationTraffic-induced vibrations have recently been of major concern to engineers as one of the aspects of so called physical environmental pollution. The interest is related to the observations of serious negative effects of such kind of vibrations on buildings and their inhabitants. Traffic-induced vibrations may cause plaster cracks, structural damage and even failure and collapse of the structure. They may also be the reason of serious...
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IN VITRO PROPAGATION OF RHODODENDRON TOMENTOSUM - AN ENDANGERED ESSENTIAL OIL BEARING PLANT FROM PEATLAND
PublicationRhododendron tomentosum Harmaja (formerly Ledum palustre L.) is a medicinal peat bog plant native to northern Europe, Asia and North America. This plant has a distinctive aroma thanks to the presence of essential oil, to which it also owes its anti-inflammatory, analgesic, antimicrobial and insecticidal properties. However, in Europe R. tomentosum is classified as an endangered species, mainly due to degradation of peatlands. In...
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Revealing dynamic changes of the volatile profile of food samples using PTR–MS
PublicationVolatile compounds carry valuable information regarding the properties of foodstuffs. Volatiles emitted from food can be used as, for example, indicators of quality, shelf-life, or authenticity. A better understanding of the multitude of transformations which occur during food processing could facilitate the optimisation of production, increase the desirability of food products, and also their wholesomeness. However, as some of...
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Recent advancements in LC‐MS based analysis of biotoxins: Present and future challenges
PublicationThere has been a rising concern regarding the harmful impact of biotoxins, source of origin, and the determination of the specific type of toxin. With numerous reports on their extensive spread, biotoxins pose a critical challenge to figure out their parent groups, metabolites, and concentration. In that aspect, liquid chromatography‐mass spectrometry (LC‐MS) based analysis paves the way for its accurate identification and quantification....
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A novel methodology for the enantiomeric resolution of methamphetamine, its precursors and intermediates by GC-MS
PublicationImpurity profiling and classification of abused drugs using chiral analytical techniques is of particular interest and importance because of the additional information obtained from this approach. When these methods are applied to the synthesis of illicitly used substances, they can supply valuable information about the conditions/chemicals used in the synthesis. In this study, simultaneous chiral separation of methylamphetamine,...
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A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells
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OZNACZANIE POZOSTAŁOŚCI PESTYCYDÓW W ORGANIZMACH PSZCZELICH ORAZ W MIODZIE Z WYKORZYSTANIEM PROCEDURY OPARTEJ NA ZASTOSOWANIU CHROMATOGRAFÓW SPRZĘŻONYCH Z TANDEMOWYMI SPEKTROMETRAMI MAS (GC-MS/MS i LC-MS/MS)
PublicationPestycydy to bardzo liczna grupa różnorodnych związków chemicznych stosowa-nych w rolnictwie. Ze względu na swoją trwałość gromadzą się w różnych elementach środowiska i mogą występować w obszarze, na którym nigdy nie zostały użyte. Zachodzi także proces ich bioakumulacji w tkankach oraz narządach zwierząt i roślin. Stosowane w rolnictwie środki ochrony roślin (szczególnie w okresie wzmożonych zabiegów agro-technicznych wiosną...
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The interaction of different abiotic conditions on the value of the component traits of the technological yield of sugar beet
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PTD4 Peptide Increases Neural Viability in an In Vitro Model of Acute Ischemic Stroke
PublicationIschemic stroke is a disturbance in cerebral blood flow caused by brain tissue ischemia and hypoxia. We optimized a multifactorial in vitro model of acute ischemic stroke using rat primary neural cultures. This model was exploited to investigate the pro-viable activity of cell-penetrating peptides: arginine-rich Tat(49–57)-NH2 (R49KKRRQRRR57-amide) and its less basic analogue, PTD4 (Y47ARAAARQARA57-amide). Our model included glucose...
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Mosty tymczasowe typu BAILEY - MS 22-80 w świetle badań in SITU
PublicationW pracy przedstawiono i omówiono zakres badań, wybrane modele obliczeniowe, wyniki analiz teoretycznych i badań odbiorowych (próbnych obciążeń o rozszerzonym zakresie), oraz spostrzeżenia dotyczące zachowania się konstrukcji na przykładzie kilku badanych obiektów mostów tymczasowych typu bailey - MS 22-80.
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Phenolic Content and Related Antioxidant Activities in in vitro Cultures of Cephalotaxus Harringtonia L. Grown in Egypt
PublicationAbstract: Quantitative estimation of the total phenolics, total flavonoids, antioxidant activity and HPLC analysis of phenolic compounds in the mother plant, in vitro shooting and callus cultures of Cephalotaxus harringtonia L. grown in Egypt were measured. For shooting behaviour, using Woody plant medium (WPM) with 2 mg/l Benzyladinin (BA) in the second subculture gave the highest number of shootlet/explant (2.33 shootlet/explant)...
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Quantification of methadone and its metabolites: EDDP and EMDP determined in autopsy cases using LC‐MS/MS
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HPLC–MS/MS method for dexmedetomidine quantification with Design of Experiments approach: application to pediatric pharmacokinetic study
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Analysis of magnetic disturbances of air platform caused by induced magnetisation
PublicationMagnetometric systems installed on air platforms like airplanes or helicopters are equipped in compensators of magnetic disturbances. Time changes of the platform's position caused generation of magnetic disturbances. The permanent and induced magnetization of ferromagnetic elements and eddy currents induced in conducting parts of the platform are sources of these disturbances. The linear model of magnetic disturbances of platforms...
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Determination of odour concentration by TD-GC×GC–TOF-MS and field olfactometry techniques
PublicationField olfactometry is one of the sensory techniques used to determine odour concentration, in atmospheric air, directly in emission sources. A two-dimensional gas chromatography with time of flight mass spectrometer (GC×GC–TOF-MS) allows performing the chemical characterization of various groups of chemical compounds, even in complex mixtures. Application of these techniques enabled determination of odour concentration level in...
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Prediction of Overall In Vitro Microsomal Stability of Drug Candidates Based on Molecular Modeling and Support Vector Machines. Case Study of Novel Arylpiperazines Derivatives
PublicationOther than efficacy of interaction with the molecular target, metabolic stability is the primary factor responsible for the failure or success of a compound in the drug development pipeline. The ideal drug candidate should be stable enough to reach its therapeutic site of action. Despite many recent excellent achievements in the field of computational methods supporting drug metabolism studies, a well-recognized procedure to model...
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Bile conjugation and its effect on in vitro lipolysis of emulsions
PublicationBile Salts (BS) are responsible for stimulating lipid digestion in our organism. Gut microbiota are responsible for the deconjugation process of primary conjugated to secondary unconjugated BS. We use two structurally distinct BS and characterize the rate of lipolysis as a compound parameter. A static in-vitro digestion model as well as meta-analysis of literature data has been performed to determine the most influential factors...
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Main strategies, analytical trends and challenges in LC-MS and ambient mass spectrometry-based metabolomics
PublicationA plethora of analytical LC-MS-based methods have been successfully applied to analyses of complex samples of various origin for metabolomics investigations. Over the last ten years, the substantial evolution of ambient mass spectrometry (AMS) techniques has highlighted their tremendous potential in metabolomic studies due to the minimal sample pretreatment requirement and the ability to analyse samples in their native state. This...
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Specificity of Infant Digestive Conditions: Some Clues for Developing Relevant In Vitro Models
PublicationDigestion of nutrients is an essential function of the newborn infant gut to allow growth and development and understanding infant digestive function is essential to optimize nutrition and oral drug delivery. Ethical considerations prohibit invasive in vivo trials and as a consequence in vitro assays are often conducted. However, the choice of in vitro model parameters are not supported by an exhaustive analysis of the literature...
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New Chalcones Derivatives With Potent Antitumor Activity And Their Metabolism In Vitro
PublicationChalcone derivatives exhibit a broad range of biological activities, with antioxidant and cytotoxic properties among the most studied. Despite numerous reports on chalcones as cytotoxic, and potentially anticancer agents, very little is know on their actual mechanism of activity. Generally, chalcones are considered as antimitotic agents, which interfere with tubulin assembly.We have recently developed a new class of chalcones with...
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Zastosowanie techniki LC-MS/MS oraz biotestów w analityce próbek środowiskowych w celu oznaczenia ich potencjału endokrynnego i zawartości wybranych związków endokrynnych
PublicationWyniki wielu badań prowadzonych w przeciągu ostatnich lat mogą stanowić podstawę do stwierdzenia, że zanieczyszczenia emitowane do środowiska ze źródeł antropogennych mogą stanowić kolejny rodzaj zagrożenia, z którego nie zdawano sobie sprawy wcześniej. Zanieczyszczeniami takimi są substancje z grupy EDC definiowane jako jakikolwiek czynnik, który wpływa na syntezę, wydalanie, transport, wiązanie, działanie lub wydalanie hormonów...
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Use of Cytokine Mix-, Imiquimod-, and Serum-Induced Monoculture and Lipopolysaccharide- and Interferon Gamma-Treated Co-Culture to Establish In Vitro Psoriasis-like Inflammation Models
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Local ordering of nano-structured Pt probed by multiple-scattering XAFS
PublicationW pracy przedstawiono wyniki analizy MS EXAFS krystalicznej platyny i nanostruktur platyny (klastry o rozmiarach od 1nm do 7nm). Rezultaty analizy masowej platyny (uwzględniające rozkłady dwu i trój-ciałowe) są zgodne z wcześniejszymi danymi strukturalnymi i wibracyjnymi i stały się podstawą do realistycznej analizy EXAFS nanocząstek Pt realizownej w połączeniu z wynikami pomiarów TEM, SEM i XRD. Badaniom poddano nanocząstki Pt...
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Nano-structured Pt embedded in the acidic salts of heteropolymolybdate matrices: MS XAFS study
PublicationMultiple-scattering extended X-ray absorption fine structure (MS EXAFS) study combined with TEM and XRD analysis of a novel Pt-based catalyst operating at low temperature fuel cells (FCs) is presented. Innovation in the case of the considered catalyst resides in the use of a porous inorganic matrix of acidic heteropolymolybdate salts as a catalyst support. Obtained results show the relation between the matrix composition and the...
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The Distribution of Glucosinolates in Different Phenotypes of Lepidium peruvianum and Their Role as Acetyl- and Butyrylcholinesterase Inhibitors—In Silico and In Vitro Studies
PublicationThe aim of the study was to present the fingerprint of different Lepidium peruvianum tu- ber extracts showing glucosinolates-containing substances possibly playing an important role in preventinting dementia and other memory disorders. Different phenotypes of Lepidium peruvianum (Brassicaceae) tubers were analysed for their glucosinolate profile using a liquid chromatograph coupled with mass spectrometer (HPLC-ESI-QTOF-MS/MS platform)....
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Validated HPTLC method for determination of ledol and alloaromadendrene in the essential oil fractions of Rhododendron tomentosum plants and in vitro cultures and bioautography for their activity screening
PublicationRhododendron tomentosum (Ledum palustre) is a bog shrub used in traditional medicine for treatment of respiratory and rheumatic diseases. Due to the large variability of the chemical composition of its essential oil, depending on the habitat, the in vitro cultures were established as the alternative source of the volatile fraction. There is a need to monitor a quality of the field grown as well as in vitro plant material, especially...
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Impact of configuration of earth continuity conductor on induced sheath voltages in power cables
PublicationIn high voltage power cable systems a problem of induced sheath voltages exist. Due to these voltages electric shock and damage of non-metallic outer sheath of the cables may occur. Exposure to voltage of the outer sheath may be very high in case of earth fault with high value of earth current. In order to reduce induced sheath voltages an earth continuity conductor (conductors) along power cables is applied. Configuration of this...
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Ball milling induced borophene flakes fabrication
PublicationTo fill the knowledge gap for borophene, as the youngest member of the two-dimensional (2D)nanomaterials family, a facile, cost effective, scalable and reproducible fabrication route is still stronglyrequired. Among so far studied techniques the potential of pure mechanical processes such as ballmilling is not explored yet. Therefore, in this contribution, we explore the efficiency...
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On Kinetic Nature of Hysteresis Phenomena in Stress-Induced Phase Transformations
PublicationA simplest model is developed which demonstrates that hysteresis phenomena in stress-induced phase transformations may have a kinetic nature and follow from the discrepancy between strain rate and characteristic rate of the new phase growth.
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A standardised static in vitro digestion method suitable for food – an international consensus
PublicationSimulated gastro-intestinal digestion is widely employed in many fields of food and nutritional sciences, as conducting human trials are often costly, resource intensive, and ethically disputable. As a consequence, in vitro alternatives that determine endpoints such as the bioaccessibility of nutrients and non-nutrients or the digestibility of macronutrients (e.g. lipids, proteins and carbohydrates) are used for screening and building...
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In vitro and in silico assessment of anti-inflammatory activity of cocoa powders
PublicationPlants are considered the major sources of biologically active compounds, which provide unlimited opportunities for their use either as medical treatments or as novel drug formulations. Cocoa powder is frequently used in nutrition and is known to have many benefits thanks to its wide range of biological activities. The presented study was focused on th evaluation of the anti-inflammatory potential of extracts obtained from cocoa...
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Earthquake-Induced Pounding Between Asymmetric Steel Buildings
PublicationEarthquake-induced pounding between buildings has been the subject of numerous numerical and experimental studies in the resent years. The phenomenon may cause severe damage to structural elements as well as may also lead to the total collapse of colliding structures. A major reason leading to pounding between adjacent, insufficiently separated buildings results from the differences in their dynamic properties. The aim of this...
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Urine metabolic fingerprinting using LC–MS and GC–MS reveals metabolite changes in prostate cancer: A pilot study
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Quantification and distribution of 4-fluoroisobutyryl fentanyl (4-FiBF) in postmortem biological samples using UHPLC–QqQ-MS/MS
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LC-PDA-MS and GC-MS Analysis of Scorzonera hispanica Seeds and Their Effects on Human Breast Cancer Cell Lines
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Earthquake-induced pounding between superstructure segments of isolated bridge
PublicationThe use of seismic isolation in bridges is considered as one of the most promising advanced technologies to enhance the structural safety during earthquakes. However, the method results in larger displacements of superstructure segments increasing the probability of pounding between them. Reports after severe ground motions show that the earthquake-induced structural pounding may lead to significant damage in isolated bridges....
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Earthquake-induced pounding between asymmetric steel buildings
PublicationEarthquake-induced pounding between buildings has been the subject of numerous numerical and experimental studies in the resent years. The phe-nomenon may cause severe damage to structural elements as well as may also lead to the total collapse of colliding structures. A major reason leading to pounding between adjacent, insufficiently separated buildings results from the differences in their dynamic properties. The aim of this...
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Cancer stem cells and escape from drug-induced premature senescence in human lung tumor cells: implications for drug resistance and in vitro drug screening models
PublicationBadania z użyciem modelu komórek nowotworu płuc A549 wykazały, że komórki te po traktowaniu lekami uszkadzającymi DNA zatrzymują proliferację i wchodzą w stan starzenia komórkowego. Jednak post-inkubacja tych komórek prowadzi do powrotu do stanu proliferacji małej frakcji komórek. Wykazaliśy, że frakcja ta związana jest z obecnością komórek pierwotnych nowotworu w populacji komórek A549. Powrót do stanu proliferacji komóek A549...
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Field-induced suppression of charge density wave in GdNiC2
PublicationWe report the specific heat, magnetic, magnetotransport, and galvanomagnetic properties of polycrystalline GdNiC 2 . In the intermediate temperature region above T N = 20 K, we observe large negative magnetoresistance due to Zeeman splitting of the electronic bands and partial destruction of a charge density wave ground state. Our magnetoresistance and Hall measurements show that at low temperatures a magnetic field-induced transformation from...
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VIBRATION PROBLEMS OF AN EXAMPLE OF TEMPORARY STEEL GRANDSTAND UNDER HUMAN-INDUCED EXCITATION
PublicationLighter and slender structural members of a typical grandstand leads to a significant reduction in the frequencies, that is why such structures are more easily induced to vibrations. The aim of the present paper is to show the results of our experimental studies and the numerical analyses, focused on the behaviour of a part of a typical temporary steel grandstand under dynamic loads. The peak values of accelerations of a steel...