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Search results for: MULTISTAGE CONSTRUCTED WETLAND SYSTEM (MCWS)
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Simulation of hybridized nanofluids flowing and heat transfer enhancement via 3-D vertical heated plate using finite element technique
PublicationThe present study probed the creation of heat energy and concentrating into Newtonian liquids across vertical 3D-heated plates. The role of the Soret and Dufour theories in concentrating and energy formulas is discussed. The role of hybrid nanoparticles is introduced to illustrate particle efciency in terms of solute and thermal energy. It is removed a viscous dissipation process and a changing magnetic feld. The proposed approach...
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Axial-Flux Permanent-Magnet Dual-Rotor Generator for a Counter-Rotating Wind Turbine
PublicationCoaxial counter-rotating propellers have been widely applied in ships and helicopters for improving the propulsion efficiency and offsetting system reactive torques. Lately, the counter-rotating concept has been introduced into the wind turbine design. Distributed wind power generation systems often require a novel approach in generator design. In this paper, prototype development of axial-flux generator with a counter-rotating...
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Calibration of acoustic vector sensor based on MEMS microphones for DOA estimation
PublicationA procedure of calibration of a custom 3D acoustic vector sensor (AVS) for the purpose of direction of arrival (DoA) estimation, is presented and validated in the paper. AVS devices working on a p-p principle may be constructed from standard pressure sensors and a signal processing system. However, in order to ensure accurate DoA estimation, each sensor needs to be calibrated. The proposed algorithm divides the calibration process...
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Superconductivity and itinerant ferromagnetism of Y9Co7 probed by ac susceptibility
PublicationThe ac magnetic susceptibility of a single crystal sample of the compound Y9Co7 has been measured in applied dc fields ranging from 0–6.7 kOe by utilizing a tunnel diode resonator circuit. In agreement with previous measurements on this material, a superconducting transition has been observed to occur at TSC ≈ 2.5 K. A broad maximum has been observed in the zero field susceptibility measurements from 2.5 K < T < 8 K and its behavior...
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Molecular transformation of dissolved organic matter in manganese ore-mediated constructed wetlands for fresh leachate treatment
PublicationThe organic matter (OM) and nitrogen in Fresh leachate (FL) from waste compression sites pose environmental and health risks. Even though the constructed wetland (CW) can efficiently remove these pollutants, the molecular-level transformations of dissolved OM (DOM) in FL remain uncertain. This study reports the molecular dynamics of DOM and nitrogen removal during FL treatment in CWs. Two lab-scale vertical-flow CW systems were...
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A New Expression System Based on Psychrotolerant Debaryomyces macquariensis Yeast and Its Application to the Production of Cold-Active β-D-Galactosidase from Paracoccus sp. 32d
PublicationYeasts provide attractive host/vector systems for heterologous gene expression. The currently used yeast-based expression platforms include mesophilic and thermotolerant species. A eukaryotic expression system working at low temperatures could be particularly useful for the production of thermolabile proteins and proteins that tend to form insoluble aggregates. For this purpose, an expression system based on an Antarctic psychrotolerant...
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Treatment Wetlands in Rural Areas of Poland for Baltic Sea Protection
PublicationWater and wastewater management, especially in rural areas, greatly affects loads of nutrients discharged to the Baltic Sea. In Poland, this management is unbalanced because of the dispersed development and in many locations construction of sewerage system is uneconomic or even impossible. For this reason, a significant part of sewage from single-family houses in rural areas must be discharged into domestic sewage systems. The...
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Fast High-Impedance Spectroscopy Method Using SINC Signal Excitation
PublicationIn this paper the method of fast impedance spectroscopy of technical objects with high impedance (|Zx| > 1 Gohm) is evaluated by means of simulation and practical experiment. The method is based on excitation of an object with a sinc signal and sampling response signals proportional to current flowing through and voltage across the measured impedance. The object impedance spectrum is obtained with use of continuous Fourier transform...
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Anatomy of noise in quantitative biological Raman spectroscopy
PublicationRaman spectroscopy is a fundamental form of molecular spectroscopy that is widely used to investigate structures and properties of molecules using their vibrational transitions. It relies on inelastic scattering of monochromatic laser light irradiating the specimen. After appropriate filtering the scattered light is dispersed onto a detector to determine the shift from the excitation wavelength, which appears in the form of...
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The application of Monod equation to denitrification kinetics description in the moving bed biofilm reactor (MBBR)
PublicationIn this paper, the kinetic constants Vmax and KCOD occurring in the Monod equation, which describe the denitrification process in the moving bed, are determined. For this purpose, a laboratory moving bed biofilm reactor (MBBR) was used. The filling of the reactor consisted of EvU Perl carriers. The experiment was carried out with an excess of nitrate, and denitrification rate was dependent on the concentration of external organic...
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System for automatic singing voice recognition
PublicationW artykule przedstawiono system automatycznego rozpoznawania jakości i typu głosu śpiewaczego. Przedstawiono bazę danych oraz zaimplementowane parametry. Algorytmem decyzyjnym jest algorytm sztucznych sieci neuronowych. Wytrenowany system decyzyjny osiąga skuteczność ok. 90% w obydwu kategoriach rozpoznawania. Dodatkowo wykazano przy pomocy metod statystycznych, że wyniki działania systemu automatycznej oceny jakości technicznej...
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Dynamika bezzałogowego aparatu latającego w układzie czterowirnikowego pionowzlotu
PublicationDziałanie maszyn, w tym również pojazdów latających, jest nieodłącznie związane z przekazywaniem oddziaływań siłowych: statycznych i dynamicznych. Dynamika jest działem mechaniki zajmującym się makroskopowym ruchem ciał przy uwzględnieniu przyczyn wywołujących ten ruch. Pierwszym etapem analizy dynamiki konstrukcji są zwykle obliczenia wartości i postaci drgań własnych modelu konstrukcji. Drgania własne zwykle ulegają szybkiemu...
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Optymalizacja strategii sieci inteligentnych agentów za pomocą programowania genetycznego w systemie rozproszonym realizującym paradygmat volunteer computing
PublicationDynamicznie rosnąca złożoność i wymagania w odniesieniu do rozproszonych systemów informatycznych utrudnia zarządzanie dostępnymi zasobami sprzętowymi i programistycznymi. Z tego powodu celem rozprawy jest opracowanie wielokryterialnej metody programowania genetycznego, która pozwala na optymalizację strategii zespołu inteligentnych agentów programistycznych w zakresie zarządzania systemem realizującym paradygmat volunteer computing....
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A Low-Cost System for Far-Field Non-Anechoic Measurements of Antenna Performance Figures
PublicationPrototype measurements are the key step in the development of antenna structures. Typically, their far-field characteristics are validated in expensive, dedicated facilities such as open range sites, or anechoic chambers. Despite being necessary for obtaining high-precision data (e.g., for device qualification), the use of costly infrastructure might not be fully justified when the main goal of measurements includes demonstration...
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Design and Optimization of Metamaterial-based Highly-isolated MIMO Antenna with High Gain and Beam Tilting Ability for 5G Millimeter Wave Applications
PublicationThis paper presents a wideband multiple-input multiple-output (MIMO) antenna with high gain and isolation, as well as beam tilting capability, for 5G millimeter wave (MMW) applications. A single bow-tie antenna fed by a substrate-integrated waveguide (SIW) is proposed to cover the 28 GHz band (26.5–29.5 GHz) with a maximum gain of 6.35 dB. To enhance the gain, H-shaped metamaterial (MM)-based components are incorporated into the...
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Cloning, expression in Komagataella phaffii, and biochemical characterization of recombinant sequence variants of Pseudomonas sp. S9 GDSL-esterase
PublicationTwo recombinant Komagataella phaffii (formerly Pichia pastoris) yeast strains for production of two sequential variants of EstS9 esterase from psychrotolerant bacterium Pseudomonas sp. S9, i.e. αEstS9N (a two-domain enzyme consisting of a catalytic domain and an autotransporter domain) and αEstS9Δ (a single-domain esterase) were constructed. However, only one of recombinant K. phaffii strains, namely Komagataella phaffii X-33/pPICZαestS9Δ,...
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Treatment of landfill leachate by constructed wetlands: three case studies
PublicationThe performance of three CW systems treating landfill leachate, two located in northern Poland (Szadółki near Gdańsk and Gatka near Miastko) and one in southern Sweden (Örebro), is discussed. The CWs differ in size, hydraulic regime, type of hydrophytic plants and type of leachate pre-treatment before discharging to the CW. The CW in Szadółki consists of two parallel beds with sub-surface, horizontal flow of leachate (HSSF) planted...
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Hybrid constructed wetlands for the National Parks in Poland - a case study, requirements, dimensioning, preliminary results
PublicationWater and wastewater management, especially in rural areas, greatly affects loads of nutrients discharged to the Baltic Sea. In Poland, this management is unbalanced because of the dispersed development and in many locations con struction of sewerage system is uneconomic or even impossible. For this reason, a significant part of sewage from single-family houses in rural areas must be discharged into domestic sewage systems. The...
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Assessment of diversity and composition of bacterial community in Sludge Treatment Reed Bed systems
PublicationDue to their low emission of odours and lack of the need to apply additional chemical agents, sludge treatment reed beds (STRBs) constitute an economically feasible and eco-friendly approach to sewage sludge management. Correctly designed and operated STRBs ensure effective reduction of the dry matter content coupled with the mineralisation of organic compounds. Successful operation of STRBs relies on complex interactions between...
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Applications of Tensor Analysis in Continuum Mechanics
PublicationA tensor field is a tensor-valued function of position in space. The use of tensor fields allows us to present physical laws in a clear, compact form. A byproduct is a set of simple and clear rules for the representation of vector differential operators such as gradient, divergence, and Laplacian in curvilinear coordinate systems. The tensorial nature of a quantity permits us to formulate transformation rules for its components...
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Combining Road Network Data from OpenStreetMap with an Authoritative Database
PublicationComputer modeling of road networks requires detailed and up-to-date dataset. This paper proposes a method of combining authoritative databases with OpenStreetMap (OSM) system. The complete route is established by finding paths in the graph constructed from partial data obtained from OSM. In order to correlate data from both sources, a method of coordinate conversion is proposed. The algorithm queries road data from OSM and provides...
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Viability of decisional DNA in robotics
PublicationThe Decisional DNA is an artificial intelligence system that uses prior experiences to shape future decisions. Decisional DNA is written in the Set Of Experience Knowledge Structure (SOEKS) and is capable of capturing and reusing a broad range of data. Decisional DNA has been implemented in several fields including Alzheimer’s diagnosis, geothermal energy and smart TV. Decisional DNA is well suited to use in robotics due to the...
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Extraction of stable foreground image regions for unattended luggage detection
PublicationA novel approach to detection of stationary objects in the video stream is presented. Stationary objects are these separated from the static background, but remaining motionless for a prolonged time. Extraction of stationary objects from images is useful in automatic detection of unattended luggage. The proposed algorithm is based on detection of image regions containing foreground image pixels having stable values in time and...
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Design and Optimization of a Compact Super-Wideband MIMO Antenna with High Isolation and Gain for 5G Applications
PublicationThis paper presents a super-wideband multiple-input multiple-output (SWB MIMO) antenna with low profile, low mutual coupling, high gain and compact size for microwave and millimeter wave (mm-wave) fifth-generation (5G) applications. A single antenna is a simple elliptical-square shape with a small physical size of 20 × 20 × 0.787 mm3. The combination of both square and elliptical shapes results in an exceptionally broad impedance...
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Life tests of a rotary single-stage magnetic-fluid seal for shipbuilding applications
PublicationUse of the magnetic fluid seal technology in water is much more difficult technological problem in comparisonto gas or vacuum environment. Some seals constructed up to time been are designed as hybrid, two stagesealing structures with shields or mechanical seals used as protection measures to the magnetic fluid seal.Anyway, there is always problem with immediate contact between magnetic fluid and the environmentalliquid. In the...
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General solution of quantum mechanical equations of motion with time-dependent Hamiltonians: A Lie algebraic approach
PublicationThe unitary operators U(t), describing the quantum time evolution of systems with a time-dependent Hamiltonian, can be constructed in an explicit manner using the method of time-dependent invariants. We clarify the role of Lie-algebraic techniques in this context and elaborate the theory for SU(2) and SU(1,1). In these cases we give explicit formulae for obtaining general solutions from special ones. We show that the constructions...
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Density functional LCAO calculations of vibrational modes and phonon density of states in the strained single-layer phosphorene
PublicationThe paper presents an investigation of phosphorene under axial strain on the phonon density of states and vibrational modes. The studies were performed by means of density functional theory (DFT) within the linear combination of atomic orbitals (LCAO). The strained models were constructed using optimised supercell techniques. The vibrational mode spectra were estimated for strains applied for both the zigzag and armchair directions...
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Procesy i efektywność usuwania zanieczyszczeń z odcieków ze składowisk odpadów komunalnych w oczyszczalniach hydrofitowych
PublicationOczyszczanie odcieków z SOK jest jednym z najbardziej istotnych problemów ekologicznych na świecie. Odprowadzenie odcieków do oczyszczalni ścieków komunalnych jest przeważnie niemożliwe, ze względu na ich zmienną ilość oraz skład, bardzo wysokie stężenia ChZT i azotu amonowego, a także obecność trwałych zanieczyszczeń organicznych (TZO) oraz metali ciężkich. Najlepszym rozwiązaniem jest oczyszczanie wód odciekowych na terenie składowiska....
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Framework for gamification of specialized subjects within engineering studies
PublicationGamification is a relatively new term forged along with the occurrence of new information technology, especially easy access to internet and smartphones. Gamification is a use of game mechanics, to modify the behavior of people in non-games situations to increase the involvement of individuals. The method is based on the pleasure that comes from overcoming the obstacles to reach next achievable level, which consists of challenges,...
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Detection of the Incoming Sound Direction Employing MEMS Microphones and the DSP
PublicationA 3D acoustic vector sensor based on MEMS microphones and its application to road traffic monitoring is presented in the paper. The sensor is constructed from three pairs of digital MEMS microphones, mounted on the orthogonal axes. Signals obtained from the microphones are used to compute sound intensity vectors in each direction. With this data, it is possible to compute the horizontal and vertical angle of an incoming sound....
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Deep Eutectic Solvents or Eutectic Mixtures? Characterization of Tetrabutylammonium Bromide and Nonanoic Acid Mixtures
PublicationDeep eutectic solvents have quickly attracted the attention of researchers because they better meet the requirements of green chemistry and thus have the potential to replace conventional hazardous organic solvents in some areas. To better understand the nature of these mixtures, as well as expand the possibilities of their use in different industries, a detailed examination of their physical properties, such as density, viscosity,...
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Size effect at aggregate level in microCT scans and DEM simulation – Splitting tensile test of concrete
PublicationThe paper describes an experimental and numerical study of size effect on concrete cylindrical specimens in splitting tensile test. Own experimental campaign was performed on specimens with 5 various diameters from D = 74, 105, 150, 192 and 250 mm with hardboard loading strips (distributed load according to standard methods) scaled proportionally to the specimen diameter. The crack opening-control system was applied to obtain the...
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Mutual Coupling Reduction in Antenna Arrays Using Artificial Intelligence Approach and Inverse Neural Network Surrogates
PublicationThis paper presents a novel approach to reduce undesirable coupling in antenna arrays using custom-designed resonators and inverse surrogate modeling. To illustrate the concept, two stand-ard patch antenna cells with 0.07λ edge-to-edge distance are designed and fabricated to operate at 2.45 GHz. A stepped-impedance resonator is applied between the antennas to suppress their mutual coupling. For the first time, the optimum values...
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Extreme weather layer method for implementation of nature-based solutions for climate adaptation: Case study Słupsk
PublicationOne of the most severe climate risks that is expected to affect all regions is related to stormwater. Climate models, constructed based on long-term trends, show that extreme weather events such as storms, cloudbursts and a large rise in sea level will be significant in the coming decades. Moreover, even the frequency and intensity of “normal” rainfall events, such as microbursts, are expected to be remarkably higher than today...
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Data-driven, probabilistic model for attainable speed for ships approaching Gdańsk harbour
PublicationThe growing demand for maritime transportation leads to increased traffic in ports. From this arises the need to observe the consequences of the specific speed ships reach when approaching seaports. However, usually the analyzed cases refer only to the statistical evaluation of the studied phenomenon or to the empirical modelling, ignoring the mutual influence of variables such as ship type, length or weather conditions. In this...
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Core loss resistance impact on sensorless speed control of an induction motor using hybrid adaptive sliding mode observer
PublicationInduction motors (IMs) experience power losses when a portion of the input power is converted to heat instead of driving the load. The combined effect of copper losses, core losses, and mechanical losses results in IM power losses. Unfortunately, the core losses in the motor, which have a considerable impact on its energy efficiency, are not taken into account by the generally employed dynamic model in the majority of the studies. Due...
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Seafloor Characterisation and Imaging Using Multibeam Sonar Data
PublicationThe approach to seafloor characterisation and imaging is presented. It relies on the combined, concurrent use of several techniques of multibeam sonar data processing. The first one is based on constructing the grey-level sonar images of seabed using the backscattering strength calculated for the echoes received in the consecutive beams. Then, the set of parameters describing the local region of sonar image is calculated. The second...
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An Experimental and Numerical Analysis of Water Hammer Phenomenon in Slurries
PublicationThe analysis of slurry transportation in pressure pipelines is important both from practical and theoretical point of view. Due to the nature of the medium, the number of problems arising in the course of design, operation, measurements and mathematical modeling is much higher compared to the cases where the flowing liquid is homogeneous. The equations describing the flow are more complex, and higher number of the parameters is...
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Fast Design Closure of Compact Microwave Components by Means of Feature-Based Metamodels
PublicationPrecise tuning of geometry parameters is an important consideration in the design of modern microwave passive components. It is mandatory due to limitations of theoretical design methods unable to quantify certain phenomena that are important for the operation and performance of the devices (e.g., strong cross-coupling effects in miniaturized layouts). Consequently, the initial designs obtained using analytical or equivalent network...
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An examination of an Antarctic soil metagenomic-derivate putative methylthioadenosine phosphorylase gene as a novel reporter gene for promoter trapping
PublicationThe rsfp gene was discovered during examination ofan Antarctic soil-derived metagenome library and encodesa bacterial methylthioadenosine phosphorylase (GenBank accession number GQ202582). In a previous study, we found that E. coli colonies expressing the rsfp gene reveal strong pink fluorescence after exposure to UV light when grown on culture medium supplemented with rhodamine B (Cieśliński et al., 2009). In contrast to that,...
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Expression of a GDSL esterase from Pseudomonas sp. S9 in Pichiapastoris
PublicationCold active lipolytic enzymes are promising to replace the conventional enzymes processes of biotechnological industries. One of the most important feature of the cold-active lipases and esterases is that they offer economic benefits through energy saving. In general, they exhibit high activity at low temperatures and low thermostability at moderate temperatures. Lipolytic enzyme EstS9 was isolated from Pseudomonas sp. S9. A multiple...
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DMSO hydration redefined: Unraveling the hydrophobic hydration of solutes with a mixed hydrophilic–hydrophobic characteristic
PublicationHydrophobic hydration of solutes with a mixed hydrophilic--hydrophobic characteristics is still poorly understood. This is because both experimental and theoretical methods find it difficult to see the ice-like water structure around the nonpolar solute groups, unlike hydrogen bonds with the hydrophilic groups. In order to unravel this problem, we have investigated DMSO hydration by means of infrared spectroscopy and theoretical...
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A vector-enzymatic DNA fragment amplification-expression technology for construction of artificial, concatemeric DNA, RNA and proteins for novel biomaterials, biomedical and industrial applications
PublicationA DNA fragment amplification/expression technology for the production of new generation biomaterials for scientific, industrial and biomedical applications is described. The technology enables the formation of artificial Open Reading Frames (ORFs) encoding concatemeric RNAs and proteins. It recruits the Type IIS SapI restriction endonuclease (REase) for an assembling of DNA fragments in an ordered head-to-tail-orientation. The...
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The treatment of wastewater containing pharmaceuticals in microcosm constructed wetlands: the occurrence of integrons (int1–2) and associated resistance genes (sul1–3, qacEΔ1)
PublicationThe aim of this study was to analyze the occurrence of sulfonamide resistance genes (sul1–3) and other genetic elements as antiseptic resistance gene (qacEΔ1) and class 1 and class 2 integrons (int1–2) in the upper layer of substrate and in the effluent of microcosm constructed wetlands (CWs) treating artificial wastewater containing diclofenac and sulfamethoxazole (SMX), which is a sulfonamide antibiotic. The bacteria in the substrate...
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Efficient production of Staphylococcus simulans lysostaphin in a benchtop bioreactor by recombinant Escherichia coli.
PublicationLysostaphin is an enzyme with bactericidal activity against Staphylococcus aureus and other staphylococcal species. In spite of many advantages and promising results of preliminary research, the enzyme is still not widely used in medicine, veterinary or as a food preservative. One of the most important factors limiting application of the enzyme in clinical or technological practice is the high costs of its production. In the present...
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Comparative analysis of three‐phase dual active bridge converter with different transformer topology and modern universal control for DC microgrids
PublicationThe presented work discusses issues related to the use of modern multiphase topologies of Dual Active Bridge (DAB)-type converters. Converters of this type are widely used in most DC microgrid applications. The introduction emphasizes a comparative analysis between single-phase and multi-phase DAB topologies within high-power DC microgrids, delving into their respective advantages, drawbacks, design procedures, and considerations...
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Recent Advances in Accelerated Multi-Objective Design of High-Frequency Structures using Knowledge-Based Constrained Modeling Approach
PublicationDesign automation, including reliable optimization of engineering systems, is of paramount importance for both academia and industry. This includes the design of high-frequency structures (antennas, microwave circuits, integrated photonic components), where the appropriate adjustment of geometry and material parameters is crucial to meet stringent performance requirements dictated by practical applications. Realistic design has...
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Computationally-Efficient Statistical Design and Yield Optimization of Resonator-Based Notch Filters Using Feature-Based Surrogates
PublicationModern microwave devices are designed to fulfill stringent requirements pertaining to electrical performance, which requires, among others, a meticulous tuning of their geometry parameters. When moving up in frequency, physical dimensions of passive microwave circuits become smaller, making the system performance increasingly susceptible to manufacturing tolerances. In particular, inherent inaccuracy of fabrication processes affect...
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Solid state versus fiber picosecond infrared lasers applied to two-photon vision tests
PublicationDevelopment of new microperimetric tools dedicated for imaging of early functional changes in the retina may help in the monitoring of various ocular diseases progression e.g. Age-Related Macular Degeneration. Recently described two-photon vision may be applied to microperimetric devices. Many subjects with well-known disease history could be investigated with newly developed instrumentation that tests ability of human eye to perceive...
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Cloning, expression, and biochemical characterization of a coldactive GDSL-esterase of a Pseudomonas sp. S9 isolated from Spitsbergen island soil
PublicationAn estS9 gene, encoding an esterase of the psychrotolerant bacterium Pseudomonas sp. S9 was cloned and sequenced. The deduced sequence revealed a protein of 636 amino acid residues with a molecular mass of 69 kDa.Further amino acid sequence analysis revealed that the EstS9 enzyme contained a G-D-S-L motif centered at a catalytic serine, an N-terminal catalytic domain and a C-terminal autotransporter domain. Two recombinant E. coli...