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A Colorimetric Microplate Assay for DNA-Binding Activity of His-Tagged MutS Protein
PublicationA simple microplate method was designed for rapid testing DNA-binding activity of proteins. The principle of the assay involves binding of tested DNA by his-tagged protein immobilized on a nickel-coated ELISA plate, following colorimetric detection of biotinylated DNA with avidin conjugated to horseradish peroxidase. The method was used to compare DNA mismatch binding activities of MutS proteins from three bacterial species. The...
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An assessment of Islamic banking in Bosnia and Herzegovina – a comparative analysis using the CAMELS approach
PublicationThis paper examines the phenomenon of Islamic banking in Bosnia and Herzegovina. Although almost half of the Bosnian population is recognized as Islamic, there is only one bank purely based on Sharia operating in this country. The aim of the article is to conduct a comparative analysis of the financial standing of this bank and the banking sector in this country and region. The chosen method of analysis is CAMELS which examines...
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Ranking of ecotoxisity tests for underground water assessment using the Hasse diagram technique
PublicationThe present study deals with the novel application of the Hasse diagram technique (HDT) for the specific ranking of ecotoxicity tests capable of assessment of underground water quality. The area studied is a multi-municipal landfill in the northern Poland. The monitoring network of the landfill constitutes of 27 piezometers for underground water monitoring and two observation points at surface water courses. After sampling, chemical...
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Accuracy Investigations of Turbine Blading Neural Models Applied to Thermal and Flow Diagnostics
PublicationPossibility of replacing computional fluid dynamics simulations by a neural model for fluid flow and thermal diagnostics of steam turbines is investigated. Results of calculations of velocity magnitude of steam for 3D model of the stator of steam turbine is presented.
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A new prototype of piezoelectric bending resonant transducer for analysis of soft tissues properties
PublicationThis paper is devoted to a new piezoelectric bending resonant transducer prototype dedicated to the characterization of the mechanical properties of soft tissue. A general description of the actuator’s structure is presented including the basic principles of the measurement. The chosen geometry of the prototype is discussed and compared with the existing version. Constitutive equations are presented for the active and passive layer...
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A chemo-mechano-thermodynamical contact theory for adhesion, friction, and (de)bonding reactions
PublicationThis work presents a self-contained continuum formulation for coupled chemical, mechanical, and thermal contact interactions. The formulation is very general and, hence, admits arbitrary geometry, deformation, and material behavior. All model equations are derived rigorously from the balance laws of mass, momentum, energy, and entropy in the framework of irreversible thermodynamics, thus exposing all the coupling present in the...
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DNAffinity: a machine-learning approach to predict DNA binding affinities of transcription factors
PublicationWe present a physics-based machine learning approach to predict in vitro transcription factor binding affinities from structural and mechanical DNA properties directly derived from atomistic molecular dynamics simulations. The method is able to predict affinities obtained with techniques as different as uPBM, gcPBM and HT-SELEX with an excellent performance, much better than existing algorithms. Due to its nature, the method can...
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Imidazo-thiazine, -diazinone and -diazepinone derivatives. Synthesis, structure and benzodiazepine receptor binding
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Analysis of Business Customers’ Energy Consumption Data Registered by Trading Companies in Poland
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Molecular insights into receptor binding energetics and neutralization of SARS-CoV-2 variants
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Sensitivity analysis of buckling loads of bisymmetric I-section columns with bracing elements
PublicationWyznaczono pierwsza wariacje siły krytycznej wyboczenia słupa o przekroju bisymetrycznym otwartym przy wariacji sztywnosci i połozenia stezen. Przyjeto załozenia klasycznej teorii pretów cienkosciennych o nieodkształcalnym przekroju poprzecznym. Analizowano zarówno stezenia boczne jak i stezenia ograniczajacespaczenie oraz skrecenie przekroju poprzecznego. Stezenia modelowano jako podpory sprezyste. W przykładzie numerycznym zbadano...
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Hybrid materials comprising trimethylglycinamide groups: immobilization consequences for anion binding affinities
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Assessment of Adjustment of GNSS Railway Measurements with Parameter-Binding Conditions in a Stationary Scenario
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NK1 receptor binding of a few low molecular weight 3,5-bistrifluoromethylbenzene derivatives
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Fentanyl Family at the Mu-Opioid Receptor: Uniform Assessment of Binding and Computational Analysis
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The crack surface morphology investigation of S355J2 steel after bending-torsion fatigue
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Minimum vertex ranking spanning tree problem for chordal and proper interval graphs
PublicationW pracy rozważamy problem szukania, dla danego grafu prostego, drzewa spinającego, którego uporządkowana liczba chromatyczna jest minimalna. K.~Miyata i inni dowiedli w [Np-hardness proof and an approximation algorithm for the minimum vertex ranking spanning tree problem,Discrete Appl. Math. 154 (2006) 2402-2410], że odpowiedni problem decyzyjny jest NP-trudny już w przypadku pytania o istnienie uporządkowanego 4-pokolorowania....
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Pareto Ranking Bisection Algorithm for Expedited Multi-Objective Optimization of Antenna Structures
PublicationThe purpose of this letter is introduction of a novel methodology for expedited multi-objective design of antenna structures. The key component of the presented approach is fast identification of the initial representation of the Pareto front (i.e., a set of design representing the best possible trade-offs between conflicting objectives) using a Pareto-ranking bisection algorithm. The algorithm finds a discrete set of Pareto-optimal...
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Utilization of the zero unitarization method for the building of a ranking for diagnostic marine engine parameters
PublicationChanging some of the parameters of the engine structure affects the emission of harmful components in the exhaust gases This primarily concerns damage in the cargo exchange system as well as in the fuel system and engine boost system. Changes in emissions of harmful compounds are often ambiguous, depending largely on the parameters that shape the combustion process. An additional problem is that often simple but undesired interactions...
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Evaluation of Asphalt Mixture Low-Temperature Performance in Bending Beam Creep Test
PublicationLow-temperature cracking is one of the most common road pavement distress types in Poland. While bitumen performance can be evaluated in detail using bending beam rheometer (BBR) or dynamic shear rheometer (DSR) tests, none of the normalized test methods gives a comprehensive representation of low-temperature performance of the asphalt mixtures. This article presents the Bending Beam Creep test performed at temperatures from −20...
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Efficient three-dimensional fluorescence measurements for characterization of binding properties in some plants
PublicationThe main aim of this research was to characterize some plants and to determine their similarities and differences, using spectroscopic methods. The interactions of soluble polyphenols of different plants with human serum albumin (HSA) were investigated by 3D-fluorescence. The obtained fluorescence results allow to classify the investigated plants according to their binding properties. The HSA-binding capacities of these plants...
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An influence of the ship's block coefficient implementation on the evaluation of it's hull girder bending
PublicationAn influence of the three different ways of implementation ship's block coefficient δ (three geometrical models) on the stresses due to wave bending moment have been investigated. Two models have been applied and compared: beam one (description of the shape usingparameters) and FEM shell model (direct representation of the shape). The outcomes have been compared to Polish Register of Shipping (PRS) rules. The results show that...
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Optimization of the efficiency of braking energy recovery in rail transport by changing arrival time
PublicationThe article refers to the previous work of the authors, in which the model of traffic organization of cooperating trains including the optimization of the use of energy returned to the catenary was presented. In the presented article, the model was modified by changing the main control variable, which affects the efficient use of energy. Departure time was changed for the arrival time of the train to the stop or station. The optimization...
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Generalized Pareto ranking bisection for computationally feasible multi-objective antenna optimization
PublicationMulti-objective optimization (MO) allows for obtaining comprehensive information about possible design trade-offs of a given antenna structure. Yet, executing MO using the most popular class of techniques, population-based metaheuristics, may be computationally prohibitive when full-wave EM analysis is utilized for antenna evaluation. In this work, a low-cost and fully deterministic MO methodology is introduced. The proposed generalized...
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Anion binding by p-aminoazobenzene-derived aromatic amides: spectroscopic and electrochemical studies
PublicationThe synthesis and complexing properties of p-aminoazobenzene-derived mono-, bis-, and trisamides were described. Ligands 3 and 4 bind anions, including fluorides, chlorides, bromides, acetates, benzoates, dihydrogen phosphates, hydrogen sulfates, and p-toluenesulfonates, in chloroform forming 1 : 1 complexes. The highest value of stability constant was evaluated for the 4-F− complex (log K = 5.63 ± 0.21). On the basis of 1H NMR,...
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Geometry optimization of steroid sulfatase inhibitors - the influence on the free binding energy with STS
PublicationIn the paper we review the application of two techniques (molecular mechanics and quantum mechanics) to study the influence of geometry optimization of the steroid sulfatase inhibitors on the values of descriptors coded their chemical structure and their free binding energy with the STS protein. We selected 22 STS-inhibitors and compared their structures optimized with MM+, PM7 and DFT B3LYP/6–31++G* approaches considering separately...
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Crystal structure, chemical bonding, and physical properties of layered AIrSn2 (A = Sr and Ba)
PublicationWe report the experimental and theoretical investigation of structure, chemical bonding interactions, and physical properties of new ternary stannides AIrSn2 (A = Sr and Ba). AIrSn2 (A = Sr and Ba) crystallizes in the orthorhombic Re3B-type structure with the space group Cmcm (No. 64). According to single-crystal X-ray diffraction results, the structure of AIrSn2 (A = Sr and Ba) can be considered as a Zintl-type compound with heterogeneous...
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A Concept of Automatic Film Color Grading Based on Music Recognition and Evoked Emotions
PublicationThe article presents the aspects of the final selection of the color of shots in film production based on the psychology of color. First of all, the elements of color processing, contrast, saturation or white balance in the film shots were presented and the definition of color grading was given. In the second part of the article the analysis of film music was conducted in the context of stimulating appropriate emotions while watching...
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Subjective tests for gathering knowledge for applying color grading to video clips automatically
PublicationThe analysis of film music concerning caused emotions may allow for a more accurate adaptation of the color of the film in the context of color grading. Therefore, this paper aims to gather knowledge on the correlation between the applied color palette to a video clip, music associated with a particular shot, and emotions evoked. For that purpose, subjective tests are prepared in which several video clips are presented with or...
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Dynamic analysis of temporary steel grandstand equipped with different types of bracing system
PublicationIn the paper, behaviour of a temporary steel grandstand equipped with two different types of bracing system has been analysed through the numerical study. A typical solution concerning application of a diagonal tubular members has been compared with elements proposed by authors and called polymer dampers. A polymer element consists of two L-shape steel members bonded with polymer mass. The aim of the paper is to verify the effectiveness...
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Analysis of temporary steel grandstand with different bracing systems exposed to crowd load
PublicationGrandstands are structures which are regularly subjected to dynamic loads generated by crowd motions. It is a dangerous situation when spectators induce rhythmic jumping, dancing, stamping, etc. If the synchronized movement of spectators excites a natural frequency of the structure, resonant response might occur. To avoid such situations, temporary steel grandstands are commonly strengthened using additional elements that create...
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Banking stability during the economic transformation process in selected countries of the Western Balkans
PublicationEconomic transformation is still an ongoing process in many European countries. Despite common economic roots, the current economic situations are developing differently in different countries. This article will consider the process of economic transformation in terms of the banking sector. It aims to assess the level of banking stability throughout the transformation process in the Western Balkan region, and to assess the determinates...
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Single-stranded DNA-binding proteins (SSBs) - sources and applicationsin molecular biology
PublicationBiałka wiążące się do jednoniciowego DNA (SSB) odgrywają ważną rolę w replikacji, rekombinacji i naprawie DNA u bakterii, archea i eukariota. Ostatnio wzrosło zainteresowanie białkami SSB jako przydatnymi narzędziami w metodach badawczych biologii molekularnej, analitycznych i diagnostycznych. Większość znanych prokariotycznych białek SSB zbudowanych jest z czterech podjednostek (homotetramer) z jedną domeną wiążąca (OB) jednoniciowe...
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SIMULATIONS OF FRACTURE IN CONCRETE BEAMS UNDER BENDING USING A CONTINUUM AND DISCRETE APPROACH
PublicationThe paper describes two-dimensional meso-scale results of fracture in notched concrete beams under bending. Concrete was modelled as a random heterogeneous 4-phase material composed of aggregate particles, cement matrix, interfacial transitional zones and air voids. Within continuum mechanics, the simulations were carried out with the finite element method based on a isotropic damage constitutive model enhanced by a characteristic...
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Analysis of Roughness, the Material Removal Rate, and the Acoustic Emission Signal Obtained in Flat Grinding Processes
PublicationIn this work, the analysis of the acoustic emission (AE) signal in grinding processes is addressed. The proposed analysis method decomposes the acoustic signal into three frequency ranges. The total energy of each range is determined, as well as the highest frequency. Different grinding experiments were carried out, according to a full factorial design of experiments (DOE), in which feed speed, depth of cut, and transversal step...
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The use of Preston equation to determine material removal during lap-grinding with electroplated CBN tools
PublicationGrinding executed in a lapping configuration is an alternative finishing process benefiting from both grinding and free-abrasive machining, while minimizing the heat effect impact. Electroplated tools can be effectively used in different abrasive processes, including high-speed grinding, however, the assessment of machining performance over time is a key factor in their correct use to achieve satisfactory technological results....
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Efficiency of hybrid equipment combining operations of surface hardening by high frequency currents and abrasive grinding
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The Influence of the Depth of Cut in Single-Pass Grinding on the Microstructure and Properties of the C45 Steel Surface Layer
PublicationThe paper contains the results of a metallographic examination and nanoindentation test conducted for the medium carbon structural steel with low content of Mn, Si, Cu, Cr, and Ni after its grinding to a depth ranging from 2 μm to 20 μm, at constant cutting speed (peripheral speed) of vs = 25 ms−1 and constant feed rate of vft = 1 m/min. Applied grinding parameters did not cause the surface layer hardening, which could generate...
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Road mortality of pond-breeding amphibians during spring migrations in the Mazurian Lakeland, NE Poland
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Enhancing seismic performance of buckling-restrained brace frames equipped with innovative bracing systems
PublicationNowadays, to improve the performance of conventional bracing systems, in which, buckling in the pressure loads is the main disadvantage, the buckling-restrained brace (BRB) is introduced as a solution. In this study, the performance of the BRB system was improved with innovative lateral-resisting systems of double-stage yield buckling-restrained brace (DYB), and a combination of DYB improved with shape memory alloy (SMA) materials...
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Effect of bending-torsion on fracture and fatigue life for 18Ni300 steel specimens produced by SLM
PublicationIn this study, different fracture surfaces caused by fatigue failure were generated from 18Ni300 steel produced by selective laser melting (SLM). Hollow round bars with a transverse hole were tested under bending-torsion to investigate the crack initiation mechanisms and fatigue life. Next, the post-failure fracture surfaces were examined by optical profilometer and scanning electron microscope. The focus is placed on the relationship...
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Coda wave interferometry in monitoring the fracture process of concrete beams under bending test
PublicationEarly detection of damage is necessary for the safe and reliable use of civil engineering structures made of concrete. Recently, the identification of micro-cracks in concrete has become an area of growing interest, especially using wave-based techniques. In this paper, a non-destructive testing approach for the characterization of the fracture process was presented. Experimental tests were made on concrete beams subjected to mechanical...
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Sensitivity, Specificity and Prioritization of Gene Set Analysis When Applying Different Ranking Metrics
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Novel Potential Binding Sites for Selective Inhibitor Design of Human Soluble Epoxide Hydrolase
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Fracture simulations in concrete beam under bending using a mesoscopic model with cohesive elements
PublicationThe main aim of this paper was to investigate a complex fracture process in a concrete beam subjected to 3-point bending test by means of the 2D meso-scale FEM with 4-node cohesive elements embedded in the initial mesh of 3-node solid elements. The material heterogeneity was taken into account by considering 3 different phases (aggregate, cement matrix, ITZs) on the basis of randomly generated internal structure of concrete and...
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Conventional and advanced bending strength analysis of common and castellated, homogeneous and hybrid I-beams
PublicationAnaliza wytrzymałości na zginanie belek dwuteowych
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Degradation of stiffness of building columns exposed to bending and impact load during ground motions
PublicationThe safety of civil engineering structures under external loads, including ground motions, is one of the issues related to the idea of sustainable society. The earthquake-induced soft-storey failure is a typical type of damage observed in buildings due to seismic excitation. The failure of an intermediate soft storey of a structure results in large vertical impact loading acting on the lower floors due to the fall of the upper...
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Novel single-stranded DNA-binding protein from psyvhrophilic bacterium Psychrobacter arcticus
PublicationTo study the biochemical properties of SSB from Psychrobacter arcticus (ParSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 642 nucleotides encoding SSB protein of 213 amino acids with a calculated molecular mass of 22.8 kDa. The amino-acid sequence of ParSSB exhibits 49% identity and 57% similarity to Escherichia coli SSB....
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Induced-fit binding of laccase to gold and carbon electrodes for the biological fuel cell applications
PublicationAnalogues of laccase natural substrates (syringic, veratric, ferulic, vanillic, isovanillic, 3,5-dimethoxybenzoic aldehydes) were employed to bind and orient laccase molecules in a way which facilitates adsorption of the catalyst molecules and their electrical connection with the conductive support. Laccase was bound efficiently to these substrates both on gold and carbon electrodes forming, respectively, 2D and 3D films sensitive...
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Unusual dynamic properties of water near the ice-binding plane of hyperactive antifreeze protein
PublicationThe dynamical properties of solvation water of hyperactive antifreeze protein from Choristoneura fumiferana (CfAFP) are analyzed and discussed in context of its antifreeze activity. The protein comprises of three well-defined planes and one of them binds to the surface of ice. The dynamical properties of solvation water around each of these planes were analyzed separately; the results are compared with the dynamical properties...