Wyniki wyszukiwania dla: antifungal%20targets
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Prediction of protein assemblies, the next frontier: The CASP14‐CAPRI experiment
PublikacjaWe present the results for CAPRI Round 50, the 4th joint CASP-CAPRI protein assembly prediction challenge. The Round comprised a total of 12 targets, including 6 dimers, 3 trimers, and 3 higher-order oligomers. Four of these were easy targets, for which good structural templates were available either for the full assembly, or for the main interfaces (of the higher-order oligomers). Eight were difficult targets for which only distantly...
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The complete stereochemistry of the antibiotic candicidin A3 (syn. ascosin A3, levorin A3)
PublikacjaHerein, the stereostructure of the aromatic heptaene macrolide (AHM) antifungal antibiotic candicidin A3 (syn. ascosin A3, levorin A3) has been established upon the 2D NMR studies, consisting of DQF-COSY, TOCSY, ROESY, HSQC and HMBC experiments, as well as upon extensive molecular dynamics simulations. The geometry of the heptaenic chromophore was defined as: (22E, 24E, 26Z, 28Z, 30E, 32E, 34E). The previously unreported absolute...
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Terrestrial Laser Scan and Survey Images - 3D Model - Simulated Car Accident - MP1 - Reigl
Dane BadawczeDataset description: Raw images from photogrammetric and terresttial laser scaning survey. Object: Simulated Car AccidentLocation: Puck Pomorskie, PolandDrone type: DJI Mavic Pro 1Flight plan: Circle | Point Of InterestTarget Product: 3D ModelDate: 07.07.2017Direct georeferencing: yesMetadata data: Yes/GPSGCP: Yes - some targets on the object are marked...
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Synthesis and antimicrobial activity of 6-sulfo-6-deoxy-D-glucosamine and its derivatives
Publikacja6-Sulfo-6-deoxy-D-glucosamine (GlcN6S), 6-sulfo-6-deoxy-D-glucosaminitol (ADGS) and their N-acetyl and methyl ester derivatives have been synthesized and tested as inhibitors of enzymes catalyzing reactions of the UDP-GlcNAc pathway in bacteria and yeasts. GlcN6S and ADGS at micromolar concentrations inhibited glucosamine-6-phosphate (GlcN6P) synthase of microbial origin. The former was also inhibitory towards fungal GlcN6P N-acetyl...
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Terrestrial Laser Scan and Survey Images - 3D Model - Submarine Kobben - Canon - Reigl
Dane BadawczeDataset description: Raw images from photogrammetric and terresttial laser scaning survey. Object: Submarine Kobben - decomisionedLocation: Gdynia, Pomorskie, PolandDrone type: n/a (Teresstrial images)Flight plan: Free - walk arround the objectTarget Product: 3D ModelDate: 05.07.2017Direct georeferencing: noMetadata data: NoGCP: N0 - some targets on...
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Review on Current Status of Echinocandins Use
PublikacjaFungal infections are rising all over the world every year. There are only five medical compound classes for treatment: triazoles, echinocandins, polyenes, flucytosine and allylamine. Currently, echinocandins are the most important compounds, because of their wide activity spectrum and much lower sides effects that may occur during therapy with other drugs. Echinocandins are secondary metabolites of fungi, which can inhibit the...
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Investigation of the uniformity of TeO2:Eu layer
Dane BadawczeTeO2 doped by Eu thin films manufactured by magnetron sputtering method were measured by XPS method. Te-Eu mosaic target with diameter of 50.8 mm was sputtered for about 45 min in argon-oxygen atmosphere what resulted in 300 nm film thickness deposition. The pressure in the deposition chamber was below 0.2 Pa and substrate was heated at 200 oC during...
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Modeling protein structures with the coarse-grained UNRES force field in the CASP14 experiment
PublikacjaThe UNited RESidue (UNRES) force field was tested in the 14th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP14), in which larger oligomeric and multimeric targets were present compared to previous editions. Three prediction modes were tested (i) ab initio (the UNRES group), (ii) contact-assisted (the UNRES- contact group), and (iii) template-assisted (the UNRES-template...
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Isolation of the GFA1 gene encoding glucosamine-6-phosphate synthase of Sporothrix schenckii and its expression in Saccharomyces cerevisiae
PublikacjaGlucosamine-6-phosphate synthase (GlcN-6-P synthase) is an essential enzyme involved in cell wall biogenesis that has been proposed as a strategic target for antifungal chemotherapy. Here we describe the cloning and functional characterization of Sporothrix schenckii GFA1 gene which was isolated from a genomic library of the fungus. The gene encodes a predicted protein of 708 amino acids that is homologous to GlcN-6-P synthases...
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Construction of glucosamine-6-phosphate synthase with an internal hexahistydyl fragment
PublikacjaL-Glutamine: D-fructose-6-phosphate aminotransferase (EC 2.6.1.16), known also as glucosamine-6-phosphate (GlcN-6-P) syntase, catalyzes the first committed step in the amino sugar biosynthetic pathway in prokaryotic and eukaryotic organisms. The enzyme carries out a complex reaction involving ammonia transfer and sugar phosphate isomerization. GlcN-6-P syntase is an important point of metabolic control of amino sugar biosynthesis...
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Influence of a lipid bilayer on the conformational behavior of amphotericin B derivatives - A molecular dynamics study.
PublikacjaAmphotericin B (AmB) is an effective but very toxic antifungal antibiotic. In our laboratory a series of AmB derivatives of improved selectivity of action was synthesized and tested. To understand molecular basis of this improvement, comparative conformational studies of amphotericin B and its two more selectivederivatives were carried out in an aqueous solution and in a lipid membrane. These molecular simulation studies revealed...
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New Analogues of Mycophenolic Acid
PublikacjaMycophenolic acid (MPA) possesses antibacterial, antifungal, antiviral, immunosuppressive and anticancer properties. It is a non-competitive and reversible inhibitor of dehydrogenase inosine-5'-monophosphate (IMPDH). This compound belongs to the immunosuppressive drugs used for the prevention of both acute and chronic transplant rejection. Until now, two derivatives of MPA have been used clinically: mycophenolate mofetil (MMF,...
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X-Ray diffraction of the metallic nanostructures
Dane BadawczeMetallic nanostructures (gold and silver) were manufactured as a thermal annealing of gold or silver thin film. Gold films with thickness of 2.8 nm were deposited on a silicon substrates using a table-top dc magnetron sputtering coater (EM SCD 500, Leica), equipped with quartz microbalance for in-situ thickness measurements. Films were deposited from...
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Optical properties of tellurium dioxide thin films
Dane BadawczeTeO2 and TeO2 doped by Eu thin films manufactured by magnetron sputtering method were measured by optical spectroscopy. Metallic Te target and Te-Eu mosaic target with diameter of 50.8 mm were sputtered for about 45 min in argon-oxygen atmosphere what resulted in 300 nm film thickness deposition. The pressure in the chamber was below 0.2 Pa and substrate...
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Determining and visualizing safe motion parameters of a ship navigating in severe weather conditions
PublikacjaThe paper presents a method of determining, organizing and displaying ship collision avoidance information, which is based on the Collision Threat Parameters Area (CTPA) technique. The method makes it possible to visualize navigational threats as well as possible collision avoidance manoeuvres. The solution is focused on supporting navigation in severe weather conditions. Normally collision avoidance decisions are made taking into...
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Rhodotorulic Acid and its Derivatives: Synthesis, Properties, and Applications
PublikacjaSiderophores are low molecular weight compounds produced by microorganisms to scavenge iron in iron-deficient environments. Rhodotorulic acid (RA), a natural hydroxamate siderophore, plays a vital role in iron acquisition for fungi and bacteria. As the simplest natural hydroxamate siderophore, RA exhibits a high affinity for ferric ions, enabling it to form stable complexes that facilitate iron uptake and transport within microorganisms....
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The Effect of Sterols on Amphotericin B Self-Aggregation in a Lipid Bilayer as Revealed by Free Energy Simulations
PublikacjaAmphotericin B (AmB) is an effective but toxic antifungal drug, known to increase the permeability of the cell membrane, presumably by assembling into transmembrane pores in a sterol-dependent manner. The aggregation of AmB molecules in a phospholipid bilayer is, thus, crucial for the drug’s activity. To provide an insight into the molecular nature of this process, here, we report an atomistic molecular dynamics simulation study...
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Positron-electron correlation-polarization potentials for the calculation of positron collisions with atoms and molecules
PublikacjaWe present correlation-polarization potentials for the calculation of scattering cross sections of positrons with atoms and molecules. The potentials are constructed from a short-range correlation term and a long-range polarization term. For the short-range correlation term we present four different potentials that are derived from multi-component density functionals. For the long-range polarization term we employ a multi-term...
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Potential of Liquid Biopsy in Papillary Thyroid Carcinoma in Context of miRNA, BRAF and p53 Mutation
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Protective Effect of Aronia Melanocarpa Polyphenols on Cadmium Accumulation in the Body: A Study in a Rat Model of Human Exposure to this Metal
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Aqueous Extracts of Selected Potentilla Species Modulate Biological Activity of Human Normal Colon Cells
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New Insights into Dietary Supplements Used in Sport: Active Substances, Pharmacological and Side Effects
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Complexation of Bioelements and Toxic Metals by Polyphenolic Compounds - Implications for Health
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Antioxidants as a Potential Preventive and Therapeutic Strategy for Cadmium
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Targeting shelterin proteins for cancer therapy.
PublikacjaAs a global health challenge, cancer prompts continuous exploration for innovative therapies that are also based on new targets. One promising avenue is targeting the shelterin protein complex, a safeguard for telomeres crucial in preventing DNA damage. The role of shelterin in modulating ataxia- telangiectasia mutated (ATM) and ataxia-telangiectasia and Rad3-related (ATR) kinases, key players in the DNA damage response (DDR),...
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Composition and antimicrobial activity of fatty acids detected in the hygroscopic secretion collected from the secretory setae of larvae of the biting midge Forcipomyia nigra (Diptera: Ceratopogonidae)
PublikacjaThe hygroscopic secretion produced by the secretory setae of terrestrial larvae of the biting midge Forcipomyia nigra (Winnertz) was analysed using gas chromatography coupled with mass spectrometry (GC-MS). The viscous secretion is stored at the top of each seta and absorbs water from moist air. GC-MS analyses (four independent tests) showed that the secretion contained 12 free fatty acids, the most abundant of which were oleic...
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Hydrazinolysis Products of Selected Sugar Lactones—Crystal Structure and Microbiological Activity
PublikacjaCommercially available lactones, as well as those synthesized by us, turned out to be good substrates for the synthesis of sugar hydrazides. The exception was L-ascorbic acid, whose hydrazinolysis led to the formation of a hydrazinium salt, not the hydrazide as expected. The structure of all compounds was confirmed by NMR and X-ray analyses. The lower durability of hydrazinium L-ascorbate was additionally confirmed by thermogravimetric...
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Engineering Candida albicans glucosamine-6-phosphate synthase for efficient enzyme purification
PublikacjaRationally designed muteins of Candida albicans glucosamine-6-phosphate synthase, an enzyme known as a promising target for antifungal chemotherapy, were constructed, overexpressed in Escherichia coli and purified to near homogeneity. To facilitate and to optimize the purification of the enzyme, three recombinant versionscontaining internal oligoHis fragments were constructed: (i) by substituting residues 343 - 348...
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Application of Maximum Lenght Sequence in Silent Sonar
PublikacjaSilent sonars are designed to reduce the distance over which their sounding pulses can be detected by intercept sonars. In order to meet this objective, we can use periodical sounding signals that have low power, a very long duration and wide spectrum. If used in the silent sonar's receiver, matched filtration ensures very good detection of motionless or slow moving targets. However, it is more difficult to detect echo signals...
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Developing a cycling subsystem as part of a sustainable mobility strategy: the case of Gdansk
PublikacjaModal share is an important component in developing sustainable transport within a city. In recent years, many cities have set modal share targets for balanced and sustainable transport modes: 30% of public transport and 30% of non-motorized (cycling and walking) modes. Gdansk strategic documents have set similar goals with some actions already taken towards those goals. The cycle network is increasing popular. Gdansk’s cycling...
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The Chitosan-Based System with Scutellariae baicalensis radix Extract for the Local Treatment of Vaginal Infections
PublikacjaScutellarie baicalensis radix, as a flavone-rich source, exhibits antibacterial, antifungal, an-tioxidant, and anti-inflammatory activity. It may be used as a therapeutic agent to treat various
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- Telomeres as targets for anticancer drug development - a computational approach
ProjektyProjekt realizowany w Wydział Chemiczny zgodnie z porozumieniem Homing Plus/2011-4/3 z dnia 2012-03-29
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Fast clutter cancellation for noise radars via waveform design
PublikacjaCanceling clutter is an important, but very expensive part of signal processing in noise radars. It is shown that considerable improvements can be made to a simple least squares canceler if minor constraints are imposed onto noise waveform. Using a combination of FPGA and CPU, the proposed scheme is capable of canceling both stationary clutter and moving targets in real-time, even for high sampling rates.
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A method of determining and visualizing safe motion parameters of a ship navigating in restricted waters
PublikacjaThe paper presents a method of displaying ship collision avoidance information which is based on an unconventional Collision Threat Parameters Area (CTPA) technique. The solution presented here extends CTPA's functionality from past works by supporting navigation in restricted waters and handling ship domains analytically instead of numerically. It visualizes potential navigational threats as well as possible collision avoidance...
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Efektywna metoda wyznaczania trasy statków żaglowych
PublikacjaZaproponowano metodę wyznaczania suboptymalnej trasy statków żaglowych poruszających się w ograniczonym akwenie morskim. Uwzględniono przy tym dynamikę tego środowiska w tym warunki pogodowe oraz charakterystyki prędkościowe statków żaglowych. Jako kryterium optymalizacji przyjęto czas żeglugi T oraz liczbę wykonanych manewrów ω. Zaprojektowano heurystyczny algorytm oraz odpowiednią aplikację wyznaczania trasy dla ustalonych punktów...
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Effect Of Resveratrol And Quercetin On Telomerase Regulation In Cancer Cells And Their Anti-Cancer Potential
PublikacjaTelomeres and telomerase are nowadays one on targets for anticancer therapy. Telomerase is expressed in ~90% of human cancer cell lines and tumor specimens, whereas its enzymatic activity is not detectable in most human somatic cells. Was found that some dietary compounds can modulate telomerase activity in cancer cells. This review summarizes the current knowledge about the effects of resveratrol and quercetin on telomerase regulation...
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Cheap Cancellation of Strong Echoes for Digital Passive and Noise Radars
PublikacjaThe problem of cancellation of strong, potentially nonstationary,echoes in noise radars and passive radars utilizing digitaltransmissions is considered. The proposed solution is a multi-stage procedure.Initial clutter estimates, obtained using the least mean squares(LMS) algorithm, are refined using specially designed filters, "matched"to spectral densities of targets and clutter. When the postprocessing filtersare noncausal, the...
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Potential applications of by-products from the coffee industry in polymer technology – Current state and perspectives
PublikacjaCoffee is one of the most popular beverages in the world, and its popularity is continuously growing, which can be expressed by almost doubling production over the last three decades. Cultivation, processing, roasting, and brewing coffee are known for many years. These processes generate significant amounts of by-products since coffee bean stands for around 50% of the coffee cherry. Therefore, considering the current pro-ecological...
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Novel anticancer strategy aimed at targeting shelterin complexes by the induction of structural changes in telomeric DNA: hitting two birds with one stone.
PublikacjaThe ends of chromosomes in mammals are composed of telomeric DNA containing TTAGGG repeats, which bind specific proteins called shelterins. This telomeric DNA together with shelterins form a cap that protects the ends of chromosomes from being recognized as sites of DNA damage and from chromosomal fusions. Many very successful antitumor drugs used in the treatment of cancer patients bind to DNA, some of them with a prominent sequence...
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CDK9: Therapeutic Perspective in HCC Therapy
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Current Challenges in Targeting Tumor Desmoplasia to Improve the Efficacy of Immunotherapy
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Evaluation of antimicrobial activities of powdered cuttlebone against Klebsiella oxytoca, Staphylococcus aureus, and Aspergillus flavus
PublikacjaBackground: The presence of medicines in the environment is considered as a serious threat to the human health. The entrance of these substances into the water sources causes soil pollution, which eventually leads to the environmental pollution and it creates some problems for the public health. Also, increasing antibiotic resistant bacteria has attracted the attention of researchers to the use of natural resources such as marine...
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Antibiotic-sterol interactions provide insight into the selectivity of natural aromatic analogues of amphotericin B and their photoisomers
PublikacjaAromatic heptaene macrolides (AHMs) belong to the group of polyene macrolide antifungal antibiotics. Members of this group were the first to be used in the treatment of systemic fungal infections. Amphotericin B (AmB), a non-aromatic representative of heptaene macrolides, is of significant clinical importance in the treatment of internal mycoses. It includes the all-trans heptaene chromophore, whereas the native AHMs contain two...
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Agnieszka Pastula Dr
OsobyAgnieszka received her masters degree in biology at Jagiellonian University (Poland). Besides standard university classes, she did multiple extracurricular research internships in both basic science and medical life sciences e.g. at Warsaw University, Jagiellonian University College of Medicine, University of Medical Sciences in Poznań and University Medical Center Utrecht, that provided her with excellent interdisciplinary training...
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Small rov to detection and identification of dangerous underwater objects
PublikacjaA small unmanned underwater vehicle (UUV) to inspection of an undersea space is presented in the paper. Its behavior is controlled by a trained pilot. Correct detection and identification of targets depends on vehicle'sprecise displacement along a predefined route. Nowadays, the UUVs are equipped with an automatic control system to execute some basic maneuvers without constant human interventions. Hence, in the paper, an autopilot...
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Etyka marketingu - czy marketing jest etyczny?
PublikacjaOd jakiegoś czasu pojawiają się krytyczne głosy wobec marketingu, jako działań nieetycznych, wycelowanych przeciwko klientom i społeczeństwu jako całości. Można stwierdzić, że wizerunek marketingu jest w tym kontekście negatywny. Celem artykułu jest próba odpowiedzi na pytanie czy marketing jest etyczny. Stwierdzono, że istotą marketingu jest dbanie o dobro interesariuszy przedsiębiorstw oraz łączenie interesu firmy, klientów...
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Optimizing Ni–Cr patterned boron-doped diamond band electrodes: Doping effects on electrochemical efficiency and posaconazole sensing performance
PublikacjaThere is growing interest in developing diamond electrodes with defined geometries such as, for example, micrometer-sized electrode arrays to acquire signals for electroanalysis. For electroanalytical sensing applications, it is essential to achieve precise conductive patterns on the insulating surface. This work provides a novel approach to boron-doped diamond patterning using nichrome masking for selective seeding on an oxidized...
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Evaluation of possibilities in identification and susceptibility testing for Candida glabrata clinical isolates with the Integral System Yeast Plus (ISYP)
PublikacjaThe aim of this study was to evaluate possibilities of correct identification and susceptibility testing of C. glabrata clinical isolates with Integral System Yeast Plus (ISYP). For species identification, as the reference method, API Candida test and species-specific PCR reactions were used. The potential of antifungal susceptibility testing by the ISYP test was compared with the Sensititre Yeast One. Whilst the reference methods...
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METHOD OF ROUTING SHIPS SAILING IN DEDICATED ENVIRONMENT
PublikacjaThe method for determining the suboptimal route of sailing vessels operating in a restricted area of the sea are proposed in the paper. The dynamics of the environment including weather conditions and speed characteristics of ships sailing are considered. As optimiza-tion criterion, measure sailing time T, and the number of maneuvers performed ω, are taken into account. An heuristic algorithm, and the appropriate application routing...
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Kavita Joshi
OsobyPursuing Doctoral of Philosophy at Gdansk University of Technology. My research interests are 3D-QSAR,Homology Modelling,Molecular docking,Molecular dynamic simulation,Antifungal and anticancer agent.