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Characterization of restriction endonucleaseAjoI fromAcinetobacter johnsonii
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ОТТОКАР УЛЬ. IN MEMORIAM
PublikacjaОттокар Уль считается одним из самых влиятельных архитекторов Австрии второй половины ХХ века. Он внес значительный вклад в развитие партисипативных методов проектирования и пост-соборной реформы сакральной архитектуры. Статья освещает его биографию, идеи и проекты, а также и публикации. Кажется, что стоит, чтобы данный архитектор присутствовал в нашей памяти.
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Wikipedia and WordNet integration based on words co-occurrences
PublikacjaThe article presents a method for automatic integration of two lexical resources: semantic dictionary WordNet and electronic encyclopaedia Wikipedia. Our goal is to add automatically an semantic tags - a WordNet synset identifier to the title of the Wikipedia article. We've analyze several different ap-proaches to these problem and implement our own solution, based on word occurrences in synsets descriptions and the article body....
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Discovering relationships between data in an enterprise information system using log analysis
PublikacjaEnterprise systems are inherently complex and maintaining their full, up-to-date overview poses a serious challenge to the enterprise architects’ teams. This problem encourages the search for automated means of discovering knowledge about such systems. An important aspect of this knowledge is understanding the data that are processed by applications and their relationships. In our previous work, we used application logs of an enterprise...
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Heat transfer during pool boiling of water, methanol and R141b on porous coated horizontal tube bundles
PublikacjaPrzedstawiono wyniki badań eksperymentalnych wrzenia wody, metanolu i R141b na poziomym pęczku rur pokrytych metaliczna powłoką porowatą. Badania przeprowadzono dla podziałek rur 1.7d oaz 2d, przy ciśnieniu atmosferycznym i podciśnieniu.
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Polyurethanes modified with natural polymers for medical application. Part II. Polyurethane/gelatin, polyurethane/starch, polyurethane/cellulose
PublikacjaThis paper is a literature overview of biomedical PUR modifications with natural polymers such as starch, cellulose and gelatin. Properties like biodegradability and biocompatibility of modified PUR cause that these materials may be used as wound dressings, tissue scaffolds, tissue implants and also vascular grafts.
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Experimental test of nonclassicality with arbitrarily low detection efficiency
PublikacjaWe theoretically introduce and experimentally demonstrate the realization of a nonclassicality test that allows for arbitrarily low detection efficiency without invoking an extra assumption of independence of the devices. Our test and its implementation is set in a prepare-and-measure scenario with an upper limit on the classical communication capacity of the channel through which the systems are communicated. The essence for our...
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Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells
PublikacjaThe specific role of size scale, surface capping, and aspect ratio of zinc oxide (ZnO) particles on toxicity toward prokaryotic and eukaryotic cells was investigated. ZnO nano and microparticles of controlled size and mor- phology were synthesized by wet chemical methods. Cytotoxicity toward mammalian cells was studied using a human osteoblast cancer cell line and antibacterial activity using Gram-negative bacteria (Escherichia...
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Novel Complementary Multiple Concentric Split Ring Resonator for Reliable Characterization of Dielectric Substrates with High Sensitivity
PublikacjaAccurate characterization of dielectric substrates with high sensitivity remains an important challenge in a variety of industrial applications. This paper proposes an innovative strategy to address this challenge by developing and optimizing a unique Complementary Multiple Concentric Split Ring Resonator (CMC-SRR). The major goal is to propose a sensor design with increased sensitivity and reliability for dielectric characterization....
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Wpływ geometrii, właściwości magnetycznych oraz sposobu magnesowania próbki na rozkład przestrzenny magnetycznego pola rozproszonego
PublikacjaPraca dotyczy problematyki wpływu wybranych czynników na rozkład przestrzenny magnetycznego pola rozproszonego (MPR). Na podstawie wyników badań uzyskanych dla próbek charakteryzujących się przewężeniem i lokalnym spadkiem przenikalności magnetycznej (spowodowanym deformacją plastyczną) stwierdzono, iż oba czynniki wywierają podobny wpływ na rozkład przestrzenny MPR. Na bazie analizy wyników pomiarów MPR oraz symulacji wykonanych...
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Heat transfer characteristics of a two-phase thermosyphon heat exchanger
PublikacjaPrzedstawiono wyniki badań wpływu geometrii parownika, rodzaju rur użytych do budowy parownika, rodzaju cieczy pośredniej oraz poziomu cieczy nad najwyższym rzędem rur na działanie dwufazowego, termosyfonowego wymiennika ciepła. Zaprezentowano algorytm obliczeń przedstawionego wymiennika.
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Dynamic Data Management Among Multiple Databases for Optimization of Parallel Computations in Heterogeneous HPC Systems
PublikacjaRapid development of diverse computer architectures and hardware accelerators caused that designing parallel systems faces new problems resulting from their heterogeneity. Our implementation of a parallel system called KernelHive allows to efficiently run applications in a heterogeneous environment consisting of multiple collections of nodes with different types of computing devices. The execution engine of the system is open for...
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Ocena wykrywalności modelowych wad wąskoszczelinowych metodą pomiaru przestrzennego rozkładu magnetycznego pola rozproszonego
PublikacjaWykazano, że metoda pomiaru natężenia metodą pomiaru natężenia pola rozproszonego (MPR) w polu szczątkowym umożliwia skuteczne wykrywanie zewnętrznych wad szczelinowych o głębokościach 0,2 mm przy długości 2,5 mm, oraz wad punktowych o średnicy mniejszej od 1mm i głębokości 0,3 mm. Zwiększenie poziomu namagnesowania (pomiary w pełnym polu magnetycznym) umożliwiło wykrywanie wad o znacznie mniejszych rozmiarach. Wykazano również,...
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Organizational Wisdom: The Impact of Organizational Learning on the Absorptive Capacity of an Enterprise
PublikacjaPurpose: In this article, we analyze the concept of organizational wisdom, indicating its key elements and verifieng the relationships between them. Design/Methodology/Approach: The study was conducted at Vive Textile Recycling Sp. z o.o in Poland. Empirical data was collected from 138 managers using the PAPI technique. Structural equation modelling (SEM) was performed to test the research hypotheses. Additionally, the significance...
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The role of microbial coagulants on the physicochemical, proteolysis, microstructure and sensory properties of low-fat Edam cheese manufactured from ultrafiltered buffalo milk
PublikacjaThis work investigates the influence of using microbial coagulants, including Rhizomucor miehei (MCR) protease and Cryphonectria parasitica (MCC) protease, on the quality characteristics of low-fat Edam cheese made from ultrafiltered buffalo milk (LFUE). Concurrently, a benchmark with calf rennet (CR) has been also performed. Throughout a 90-day ripening period, the cheeses were assessed for their physicochemical features, proteolysis,...
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Wpływ sposobu obciążenia na nośność murowych elementów zespolonych
PublikacjaNa podstawie badań eksperymentalnych oceniano wpływ sposobu obciążenia murowych elementów zespolonych na nośność tych elementów. W elementach składających się z muru i zespolonego betonowego słupka obciążenie przykładano do muru i osobno do słupka. Zaobserwowano znaczne różnice nośności.
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Exploring Relationships Between Data in Enterprise Information Systems by Analysis of Log Contents
PublikacjaEnterprise systems are inherently complex and maintaining their full, up-to-date overview poses a serious challenge to the enterprise architects’ teams. This problem encourages the search for automated means of discovering knowledge about such systems. An important aspect of this knowledge is understanding the data that are processed by applications and their relationships. In our previous work, we used application logs of an enterprise...
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Cross-Lingual Knowledge Distillation via Flow-Based Voice Conversion for Robust Polyglot Text-to-Speech
PublikacjaIn this work, we introduce a framework for cross-lingual speech synthesis, which involves an upstream Voice Conversion (VC) model and a downstream Text-To-Speech (TTS) model. The proposed framework consists of 4 stages. In the first two stages, we use a VC model to convert utterances in the target locale to the voice of the target speaker. In the third stage, the converted data is combined with the linguistic features and durations...
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Improving Traffic Light Recognition Methods using Shifting Time-Windows
PublikacjaWe propose a novel method of improving algorithms recognizing traffic lights in video sequences. Our focus is on algorithms for applications which notify the driver of a light in sight. Many existing methods process images in the recording separately. Our method bases on the observation that real-life videos depict underlying continuous processes. We named our method FSA (Frame Sequence Analyzed). It is applicable for any underlying...
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Efekty egoistycznych ataków na poziomie mac w sieciach ad hoc o topologii wieloskokowej
PublikacjaStacje sieci ad hoc mogą manipulować parametrami dostępu do medium transmisyjnego dla osiągnięcia nienależnie wysokiej jakości usług. Dla środowiska sieci ad hoc o topologii wieloskokowej rozważane są ataki na mechanizmy odłożenia transmisji i klasyfikacji ruchu, przeprowadzane zarówno w stosunku do przepływów źródłowych jak i tranzytowych. Wyniki pozwalają wstępnie ocenić efekty takich ataków oraz skuteczność mechanizmów obronnych.
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Genome Mining and Evaluation of the Biocontrol Potential of Pseudomonas fluorescens BRZ63, a New Endophyte of Oilseed Rape (Brassica napus L.) against Fungal Pathogens
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Preclusion of irreversible destruction of Dr adhesin structures by a high activation barrier for the unfolding stage of the fimbrial DraE subunit
PublikacjaFimbrie Dr uropatogennych szczepów Escherichia coli stanowią przykład eksponowanych powierzchniowo struktur adhezyjnych podlegających biogenezie według zakonserwowanego, pośród bakterii Gram-ujemnych, systemu "chaperone-usher" ("białko opiekuńcze-zewnątrzbłonowe białko kanałowe"). Powyższe struktury adhezyjne są niezbędne w procesie specyficznej adhezji bakterii do receptorów zlokalizowanych na powierzchni docelowych komórek gospodarza....
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PCR melting profile (PCR MP) - a new tool for differentiation of Candida albicans strains
PublikacjaPo raz pierwszy przedstawiliśmy użyteczność techniki PCR MP opartej na metodzie ligacji adaptorów (LM PCR) i obniżonej temperaturze denaturacji w cyklach PCR do wewnątrzgatunkowego różnicowania szczepów Candida albicans. Przebadaliśmy 123 szczepy Candida albicans z zastosowaniem trzech metod genotypowania: PCR MP, REA-PFGE i RAPD i uzyskaliśmy odpowiednio 27, 26 i 25 unikalnych genotypów. Zaprezentowane dane pokazały, że technika...
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Type II secretory pathway for surface secretion of DraD invasin from the uropathogenic Escherichia coli Dr+ strain
PublikacjaWłaściwości wirulentne uropatogennych szczepów Escherichia coli Dr+ IH11128 związane są z obecnością struktur fimbrialnych typu Dr i białka inwazyny DraD, które może na powierzchni komórki bakteryjnej pełnić rolę fimbrialnej podjednostki szczytowej. Ostatnie badania wykazały, że białko DraD jest eksponowane na powierzchni bakterii w dwóch formach: związanej ze strukturą fimbrialną i jako niezależna od fimbrii otoczka białkowa....
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The noncanonical disulfide bond as the important stabilizing element of the immunoglobulin fold of the Dr fimbrial DraE subunit
PublikacjaAdhezyny fimbrialne bakterii patogennych stanowią liniowe asocjaty białkowe odpowiedzialne za wiązanie do specyficznych receptorów na powierzchni komórek gospodarza. Biogeneza tych struktur odbywa się przez system sekrecji typu chaperone/usher zakonserwowany pośród bakterii Gram-ujemnych. Powyższe struktury adhezyjne charakteryzują się wysoką odpornością na denaturację i deasocjację, powodowane przez temperaturę lub chemiczne czynniki...
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ADSRRS-fingerprinting and PCR MP techniques for studies of intraspecies genetic relatedness in Staphylococcus aureus
PublikacjaW pracy pokazano użyteczność dwóch nowych metod do typowania molekularnego Staphylococcus aureus, ADSRRS-fingerprinting (Amplification of DNA fragments Surrounding Rare Restriction Sites) i PCR MP (PCR melting profile). Przebadano 37 szczepów Staphylococcus aureus izolowanych od pacjentów z czyracznością. Jako metodę referencyjną zastosowano REA-PFGE. Okazało się, że izolaty Staphylococcus aureus z nosa od niektórych pacjentów...
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Isolation and identification of the restriction endonuclease Ptal fromPhormidium tadzschicicum, an isoschizomer of BspMII
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Investigation of interaction between xanthene dye rhodamine B and metagenomic-derivated methylthioadenosine phosphorylase RSFP
PublikacjaIn the previous study we found that the expression of the metagenomic - derived methylthioadenosine phosphorylase gene (rsfp) in E. coli cells and the presence of rhodamine B in the growth medium were crucial for the pink fluorescence of one E. coli colony from Antarctic soil metagenomic library (Cieśliński et al., 2009). In this study, rsfp gene expression was induced by an arabinose promoter in an LMG194 E. coli strain and the...
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A new MIC1-MAG1 recombinant chimeric antigen can be used instead of the Toxoplasma lysate antigen in the serodiagnosis of human toxoplasmosis
PublikacjaW niniejszej publikacji po raz pierwszy przedstawiono otrzymanie i oszacowanie przydatności diagnostycznej nowego chimerycznego antygenu MIC1-MAG1 Toxoplasma gondii do rozpoznawania toksoplazmozy u ludzi. Antygen chimeryczny MIC1-MAG1 został otrzymany jako białko fuzyjne zawierające dwie domeny oligohistydynowe na N- i C-końcu z użyciem systemu ekspresyjnego Escherichia coli. Po oczyszczeniu z wykorzystaniem metody chromatografii...
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A study on the interaction of rhodamine B with methylthioadenosine phosphorylase protein sourced from an Antarctic soil metagenomic library.
PublikacjaThe presented study examines the phenomenon of the fluorescence under UV light excitation (312 nm) of E. coli cells expressing a novel metagenomic-derived putative methylthioadenosine phosphorylase gene, called rsfp, grown on LB agar supplemented with a fluorescent dye rhodamine B. For this purpose, an rsfp gene was cloned and expressed in an LMG194 E. coli strain using an arabinose promoter. The resulting RSFP protein was purified...
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Biochemical characteristic of biofilm of uropathogenic Escherichia coli Dr+ strains.
PublikacjaUrinary tract infections caused by Escherichia coli are very common health problem in the developed countries. The virulence of the uropathogenic E. coli Dr+ IH11128 is determined by Dr fimbriae, which are homopolymeric structures composed of DraE subunits with the DraD protein capping the fiber. In this study, we have analyzed the structural and biochemical properties of biofilms developed by E. coli strains expressing Dr fimbriae...
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Dispersive Delay Structures With Asymmetric Arbitrary Group-Delay Response Using Coupled-Resonator Networks With Frequency-Variant Couplings
PublikacjaThis article reports the design of coupled-resonatorbased microwave dispersive delay structures (DDSs) with arbitrary asymmetric-type group delay response. The design process exploits a coupling matrix representation of the DDS circuit as a network of resonators with frequency-variant couplings (FVCs). The group delay response is shaped using complex transmission zeros (TZs) created by dispersive cross-couplings. We also present an...
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Inline Microwave Filters With N+1 Transmission Zeros Generated by Frequency-Variant Couplings: Coupling-Matrix-Based Synthesis and Design
PublikacjaA general coupling-matrix-based synthesis methodology for inline Nth-order microwave bandpass filters (BPFs) with frequency-variant reactive-type couplings that generate N+1 transmission zeros (TZs) is presented in this brief. The proposed approach exploits the formulation of the synthesis problem as three inverse nonlinear eigenvalue problems (INEVPs) so that the coupling matrix is built from their sets of eigenvalues. For this...
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Applications of Additively Manufactured Tools in Abrasive Machining—A Literature Review
PublikacjaHigh requirements imposed by the competitive industrial environment determine the development directions of applied manufacturing methods. 3D printing technology, also known as additive manufacturing (AM), currently being one of the most dynamically developing production methods, is increasingly used in many different areas of industry. Nowadays, apart from the possibility of making prototypes of future products, AM is also used...
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Study of the Effect of Filling Thermoplastic Medical Polyurethane with PVA, PLA or Diatomite on the Relaxation Times Distributions of 1H NMR
PublikacjaIn this work, to characterize the mobility of diferent sections of the macromole‑ cules of polyurethane (PUR), polyvinyl alcohol (PVA), and polylactic acid (PLA), as well as the density of crosslinks of the polymer chains when using fllers, we used the distributions of spin–lattice and spin–spin relaxation times for the protons. It is shown that the rigidity of the thermoplastic polymers depends on the sizes of the granules of...
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Informal work in Poland - a regional approach
PublikacjaIn our paper we try to combine the theories explaining the prevalence of informal work, using a regional approach. Reviewing the literature, we observe that, there is no evidence to confirm that the relationships that occur in cross-country comparisons remain significant when we use regional data and analyse cross-regional variations. Our general question is therefore whether there is simply more informal work in less-developed...
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Limiting distribution of Lempel Ziv'78 redundancy
PublikacjaWe show that the Lempel Ziv'78 redundancy rate tends to a Gaussian distribution for memoryless sources. We accomplish it by extending findings from our 1995 paper [3]. We present a new simplified proof of the Central Limit Theorem for the number of phrases in the LZ'78 algorithm. As in our 1995 paper, here we first analyze the asymptotic behavior of the total path length in a digital search tree (a DST) built from independent sequences....
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On the Limiting distribution of Lempel Ziv'78 Redundancy for Memoryles Sources
PublikacjaWe show that the Lempel Ziv'78 redundancy rate tends to a Gaussian distribution for memoryless sources. We accomplish it by extending findings from our 1995 paper [3]. We present a new simplified proof of the Central Limit Theorem for the number of phrases in the LZ'78 algorithm. As in our 1995 paper, here we first analyze the asymptotic behavior of the total path length in a digital search tree (a DST) built from independent sequences....
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A survey of automatic speech recognition deep models performance for Polish medical terms
PublikacjaAmong the numerous applications of speech-to-text technology is the support of documentation created by medical personnel. There are many available speech recognition systems for doctors. Their effectiveness in languages such as Polish should be verified. In connection with our project in this field, we decided to check how well the popular speech recognition systems work, employing models trained for the general Polish language....
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Low energy elastic scattering of electrons from hexafluoropropene (C3F6)
PublikacjaWe present cross sections from a joint experimental and theoretical study on elastic electron scattering from hexafluoropropene (C3F6) in the gas phase. The experimental results, using low energy electron spectroscopy, were obtained at incident electron energies of 0.5, 1, 1.5, 2, 3, 4, 5, 6, 10, 12, 15 and 20 eV, for scattering angles ranging from 10°to 130°. The theoretical method used in the computation of the integral, momentum...
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Cost-Efficient Multi-Objective Design of Miniaturized Microwave Circuits Using Machine Learning and Artificial Neural Network
PublikacjaDesigning microwave components involves managing multiple objectives such as center frequencies, impedance matching, and size reduction for miniaturized structures. Traditional multi-objective optimization (MO) approaches heavily rely on computationally expensive population-based methods, especially when exe-cuted with full-wave electromagnetic (EM) analysis to guarantee reliability. This paper introduces a novel and cost-effective...
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Music Information Retrieval – Soft Computing versus Statistics . Wyszukiwanie informacji muzycznej - algorytmy uczące versus metody statystyczne
PublikacjaMusic Information Retrieval (MIR) is an interdisciplinary research area that covers automated extraction of information from audio signals, music databases and services enabling the indexed information searching. In the early stages the primary focus of MIR was on music information through Query-by-Humming (QBH) applications, i.e. on identifying a piece of music by singing (singing/whistling), while more advanced implementations...
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Unsupervised machine-learning classification of electrophysiologically active electrodes during human cognitive task performance
PublikacjaIdentification of active electrodes that record task-relevant neurophysiological activity is needed for clinical and industrial applications as well as for investigating brain functions. We developed an unsupervised, fully automated approach to classify active electrodes showing event-related intracranial EEG (iEEG) responses from 115 patients performing a free recall verbal memory task. Our approach employed new interpretable...
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Video Semantic Analysis Framework based on Run-time Production Rules - Towards Cognitive Vision
PublikacjaThis paper proposes a service-oriented architecture for video analysis which separates object detection from event recognition. Our aim is to introduce new tools to be considered in the pathway towards Cognitive Vision as a support for classical Computer Vision techniques that have been broadly used by the scientific community. In the article, we particularly focus in solving some of the reported scalability issues found in current...
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Structural, thermal and physico-mechanical properties of polyurethane/brewers’ spent grain composite foams modified with ground tire rubber
PublikacjaIn this work, brewers’ spent grain (BSG) and ground tire rubber (GTR) waste fillers were applied as low-cost reinforcement phase in rigid polyurethane foam (PUR). PUR/BSG/GTR composites were prepared by a single step method, using polyglycerol as partial substitute of commercially available petrochemical polyols. Foaming parameters, chemical structure, dynamic mechanical properties, thermal stability, physico-mechanical properties...
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Coupled nonlinear Schrödinger equations in optic fibers theory
PublikacjaIn this paper a detailed derivation and numerical solutions of CoupledNonlinear Schr¨odinger Equations for pulses of polarized electromagnetic wavesin cylindrical fibers has been reviewed. Our recent work has been compared withsome previous ones and the advantage of our new approach over other methods hasbeen assessed. The novelty of our approach lies is an attempt to proceed withoutloss of information within the frame of basic...
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Structure of EstA esterase from psychrotrophic Pseudoalteromonas sp. 643A covalently inhibited by monoethylphosphonate.
PublikacjaThe crystal structure of the esterase EstA from the cold-adapted bacteriumPseudoalteromonas sp. 643A was determined in a covalently inhibited form at aresolution of 1.35 A˚. The enzyme has a typical SGNH hydrolase structureconsisting of a single domain containing a five-stranded beta-sheet, with threehelices at the convex side and two helices at the concave side of the sheet, and isornamented with a couple of very short helices...
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Identification and molecular modeling of a novel lipase from an Antarctic soil metagenomic library.
PublikacjaIn this work, we present the construction of a metagenomic library in Escherichia coli using pUC19 vector and environmental DNA directly isolated from Antarctic topsoil and screened for lipolytic enzymes. The screening on agar supplemented with olive oil and rhodamine B revealed one clone with lipolytic activity (Lip1) out of 11,000 E. coli clones. This clone harbored a plasmid, pLip1, which has an insert of 4722 bp that has been...
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Molecular typing of clinical isolates of Escherichia coli by a new PCR MP method
PublikacjaTypowanie epidemiologiczne bakterii jest rutynową procedurą stosowaną w badaniach zakażeń szpitalnych i wymaga stosowania odpowiednich metod o wysokim potencjale różnicującym. Przedstawiliśmy możliwości nowej techniki PCR MP w różnicowaniu szczepów E. coli. Rezultaty zostały porównane z wynikami uzyskanymi metodą PFGE.
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Molecular typing of clinical isolates of Escherichia coli by a new PCR MP method
PublikacjaTypowanie epidemiologiczne bakterii jest rutynową procedurą stosowaną w badaniach zakażeń szpitalnych i wymaga stosowania odpowiednich metod o wysokim potencjale różnicującym. Przedstawiliśmy możliwości nowej techniki PCR MP w różnicowaniu szczepów E. coli. Rezultaty zostały porównane z wynikami uzyskanymi metodą PFGE.