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Frequent Sequence Mining in Web Log Data
PublikacjaThe amount of information available even on a single web server can be huge. On the other hand, the amount of visitors (users) can often reach a number of at least six digits. Users vary in gender, age and education, and in consequence their information needs are different. Moreover, they subconsciously expect to get more adequate content after visiting the first few pages. The scope of this kind of problem relates to the domain...
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The extended version of restriction analysis approach for the examination of the ability of low-molecular-weight compounds to modify DNA in a cell-free system
PublikacjaOne of the primary requirements in toxicology is the assessment of ability of chemicals to induce DNA covalent modification. There are several well-established methods used for this purpose such as 32P-Postlabeling or HPLC-MS. However, all of these approaches have difficult to overcome limitations, which prevents their use in genotoxin screening. Here, we describe the simple protocol exploiting specificity of restriction enzymes...
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Evolution of magnetic and transport properties in (Cr1−xMnx)2AlC MAX-phase synthesized by arc melting technique
PublikacjaHerein we initiate a comeback to the arc melting technique to produce MAX-phase solid solutions. Bulk samples of (Cr1−xMnx)2AlC MAX-phase with X = 0, 0.025, 0.05 and 0.1 were synthesized and studied by means of X-ray diffractometry, scanning electron microscopy in combination with energy-dispersive X-ray spectroscopy. Samples were established to be homogeneous with an incorporation of Cr7C3, AlCr2 and Al2O3 secondary phases which...
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Multi-fidelity EM simulations and constrained surrogate modelling for low-cost multi-objective design optimisation of antennas
PublikacjaIn this study, a technique for low-cost multi-objective design optimisation of antenna structures has been proposed. The proposed approach is an enhancement of a recently reported surrogate-assisted technique exploiting variable-fidelity electromagnetic (EM) simulations and auxiliary kriging interpolation surrogate, the latter utilised to produce the initial approximation of the Pareto set. A bottleneck of the procedure for higher-dimensional...
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THE PROBLEM OF AIRFLOW AROUND BUILDINGS CLUSTERS IN DIFFERENT CONFIGURATIONS
PublikacjaIn the paper, the authors discuss the construction of a model of an exemplary urban layout. Numerical simulation has been performed by means of a commercial software Fluent using two different turbulence models: the popular k-ε realizable one, and the Reynolds Stress Model (RSM), which is still being developed. The former is a 2-equations model, while the latter – is a RSM model – that consists of 7 equations. The studies have...
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A Broadband Circularly Polarized Wide-Slot Antenna with a Miniaturized Footprint
PublikacjaThis letter presents a novel and simple feeding technique for exciting orthogonal components in a wide-slot antenna. In this technique, a rectangular bracket-shape parasitic strip is placed at the open end of the straight microstrip line to excite the fundamental horizontal and vertical components of the circular polarization (CP). The proposed technique—when employed in conjunction with the asymmetrical geometry of coplanar waveguide...
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Effect of hydroxylated soybean oil and bio-based propanediol on the structure and thermal properties of synthesized bio-polyurethanes
PublikacjaThe aim of this work was to obtain bio-polyurethanes using synthetic compounds and bio-components, i.e. bio-glycols. Bio-polyurethanes were prepared by means of the prepolymer method. Prepolymers were synthesized from 4,4′-diphenylmethane diisocyanate (MDI) and a polyol mixture containing 75% by weight of commercial polyether and 25% by weight of hydroxylated soybean oils (H2 or H3), the latter being obtained in the reaction with...
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Code development of a DSP-FPGA based control platform for power electronics applications
PublikacjaThis paper focuses on the implementation of power electronics algorithms in control platforms based on DSP-FPGA. Today’s power electronics technology demands high power computation with high speed interfacing at the same time. The most popular configuration is a DSP for the former and a FPGA for the latter. The main goal of this work was to develop a generic control system for power electronics application, but it is explained...
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Effect of urea and glycine betaine on the hydration sphere of model molecules for the surface features of proteins
PublikacjaWater properties may significantly affect protein stability. Osmolytes are compounds that intrinsically affect water in many different ways and thus can influence proteins with this type of indirect mechanism. In this study, we characterize water properties in ternary solutions: model–water–osmolyte, with two model molecules: N-methylacetamide (NMA) and dimethyl sulfoxide (DMSO) and two osmolytes: glycine betaine (TMG)and urea....
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Behavior of residual current devices at frequencies up to 50 kHz
PublikacjaThe use of residual current devices (RCDs) is obligatory in many types of low-voltage circuits. They are devices that ensure protection against electric shock in the case of indirect contact and may ensure additional protection in the case of direct contact. For the latter purpose of protection, only RCDs of a rated residual operating current not exceeding 30 mA are suitable. Unfortunately, modem current-using equipment supplied...
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QRS Morphology-Based EDR Signal—Factors Determining its Properties
PublikacjaRespiration-induced signals contain a clinically significant information. It could be obtained utilizing both direct and indirect methods. ECG-Derived Respiration (EDR) method is the latter one. However, in this case, two approaches could be distinguished. First one is based on determining changes in the morphology of QRS complexes while the second one is based on the Respiratory Sinus Arrhythmia (RSA) mechanism. The former approach...
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Computing algebraic transfer entropy and coupling directions via transcripts
PublikacjaMost random processes studied in nonlinear time series analysis take values on sets endowed with a group structure, e.g., the real and rational numbers, and the integers. This fact allows to associate with each pair of group elements a third element, called their transcript, which is defined as the product of the second element in the pair times the first one. The transfer entropy of two such processes is called algebraic transfer...
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Bimodal deep learning model for subjectively enhanced emotion classification in films
PublikacjaThis research delves into the concept of color grading in film, focusing on how color influences the emotional response of the audience. The study commenced by recalling state-of-the-art works that process audio-video signals and associated emotions by machine learning. Then, assumptions of subjective tests for refining and validating an emotion model for assigning specific emotional labels to selected film excerpts were presented....
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MERPSYS: An environment for simulation of parallel application execution on large scale HPC systems
PublikacjaIn this paper we present a new environment called MERPSYS that allows simulation of parallel application execution time on cluster-based systems. The environment offers a modeling application using the Java language extended with methods representing message passing type communication routines. It also offers a graphical interface for building a system model that incorporates various hardware components such as CPUs, GPUs, interconnects...
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Internet photogrammetry for inspection of seaports
PublikacjaThis paper intends to point out the possibility of using Internet photogrammetry to construct 3D models from the images obtained by means of UAVs (Unmanned Aerial Vehicles). The solutions may be useful for the inspection of ports as to the content of cargo, transport safety or the assessment of the technical infrastructure of port and quays. The solution can be a complement to measurements made by using laser scanning and traditional...
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Mineral Composition of Dietary Supplements-Analytical and Chemometric Approach
PublikacjaThere is a lack of data on the actual composition and effectiveness of beetroot-based dietary supplements. The research aimed to determine the profile of 22 elements (Na, K, Ca, Mg, P, Fe, As, Se, Zn, Cu, Ag, Co, Ni, Mo, Al, Mn, Sr, Cr, Ba, Li, Pb, Cd) in beetroot and its supplements by the microwave plasma atomic emission spectrometry (MP-AES) method. The analytical procedure was optimised and validated. The composition of both...
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A nosocomial outbreak of Candida parapsilosis in southern Sweden verified by genotyping
PublikacjaIn a haematology ward, Candida parapsilosis was found in blood cultures from 4 patients within a month. As C. parapsilosis is known to have a restricted genetic diversity, a combined methodological approach was adopted to establish a possible epidemiological relationship among the isolates (n = 9). Multilocus sequence typing and random amplified polymorphic DNA analysis suggested a clonal origin of the isolates. The clonal origin...
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Capillary gas chromatography using a -cyclodextrin forenantiomeric separation of methylamphetamine, its precursors andchloro intermediates after optimization of the derivatization reaction
PublikacjatThe enantiomeric ratio of methylamphetamine (MAMP) is closely related to the optical activity of precur-sors and reagents used for the synthesis and this knowledge can provide useful information concerningthe origins and synthetic methods used for illicit manufacture. The information can be utilized for reg-ulation of the precursors and investigation of the manufacturing sources but this requires analyticalprocedures to determine...
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Long Distance Geographically Distributed InfiniBand Based Computing
PublikacjaCollaboration between multiple computing centres, referred as federated computing is becom- ing important pillar of High Performance Computing (HPC) and will be one of its key components in the future. To test technical possibilities of future collaboration using 100 Gb optic fiber link (Connection was 900 km in length with 9 ms RTT time) we prepared two scenarios of operation. In the first one, Interdisciplinary Centre for Mathematical...
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Catalytic Mechanism of Non-Target DNA Cleavage in CRISPR-Cas9 Revealed by Ab Initio Molecular Dynamics
PublikacjaCRISPR-Cas9 is a cutting-edge genome editing technology, which uses the endonuclease Cas9 to introduce mutations at desired sites of the genome. This revolutionary tool is promising to treat a myriad of human genetic diseases. Nevertheless, the molecular basis of DNA cleavage, which is a fundamental step for genome editing, has not been established. Here, quantum–classical molecular dynamics (MD) and free energy methods are used...
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Multivariate Statistical Approach for Nephrines in Womenwith Obesity
PublikacjaCatecholamines are physiological regulators of carbohydrate and lipid metabolism during stress, but their chronic influence on metabolic changes in obese patients is still not clarified. The present study aimed to establish the associations between the catecholamine metabolites and metabolic syndrome (MS) components in obese women as well as to reveal the possible hidden subgroups of patients through hierarchical cluster analysis...
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Ultrasensitive electrochemical determination of the cancer biomarker protein sPD-L1 based on a BMS-8-modified gold electrode
PublikacjaThis work describes the modification of a gold electrode with the BMS-8 compound that interacts with the Programmed Death-Ligand 1 (PD-L1), an immune checkpoint protein. The results show that we can confirm the presence of the sPD-L1 in the concentration range of 10−18 to 10−8 M using electrochemical impedance spectroscopy (EIS) with a limit of detection (LOD) of 1.87 × 10−14 M for PD-L1 (S/N = 3.3) and at a concentration of 10−14...
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Risk assessment for tram traffic on tramway bridges
PublikacjaMiejski transport szynowy wielu miastach stanowi istotny element systemu transportowego i jest przestrzennie rozwijającym się systemem zapewniającym mieszkańcom codzienną obsługę transportową. Bezpieczeństwo pasażerów transportu szynowego i użytkowników dróg jest jednym z najważniejszych czynników, który należy uwzględnić w trakcie projektowania infrastruktury oraz w ocenie operacyjnej systemu miejskiego transportu tramwajowego....
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Application of Multivariate Adaptive Regression Splines (MARSplines) Methodology for Screening of Dicarboxylic Acids Cocrystal Using 1D and 2D Molecular Descriptors
PublikacjaDicarboxylic acids (DiAs) are probably one of the most popular cocrystals formers. Due to the high hydrophilicity and non-toxicity, they are promising solubilizes of active pharmaceutical ingredients (APIs). Although DiAs appear to be highly capable of forming multicomponent crystals with various compounds, some systems reported in the literature are physical mixtures the solid state without forming stable intermolecular complex....
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Predicting Value of Binding Constants of Organic Ligands to Beta-Cyclodextrin: Application of MARSplines and Descriptors Encoded in SMILES String
PublikacjaThe quantitative structure–activity relationship (QSPR) model was formulated to quantify values of the binding constant (lnK) of a series of ligands to beta–cyclodextrin (β-CD). For this purpose, the multivariate adaptive regression splines (MARSplines) methodology was adopted with molecular descriptors derived from the simplified molecular input line entry specification (SMILES) strings. This approach allows discovery of regression...
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Time Synchronization between the eNBs in E-UTRAN under the Asymmetric IP Network
PublikacjaIn this paper, we present a method for a time synchronization between the two eNodeBs (eNBs) in E-UTRAN (Evolved Universal Terrestrial Radio Access) network. The...
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Determination of pressure drop in flow condensation of HFE7000 in minichannels with account of non-adiabatic effects
PublikacjaFlow boiling and flow condensation are often regarded as two opposite or symmetrical phenomena involving the change of phase. There is a temptation to describe both these phenomena with one only correlation, however such method has yet to be suggested. From amongst the structures present in flow boiling and flow condensation at least the annular flow structure seems to be mostly appropriate to the common modeling. However, the shear...
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Performance of extended Lagrangian schemes for molecular dynamics simulations with classical polarizable force fields and density functional theory
PublikacjaIterative energy minimization with the aim of achieving self-consistency is a common feature of Born-Oppenheimer molecular dynamics (BOMD) and classical molecular dynamics with polarizable force fields. In the former, the electronic degrees of freedom are optimized, while the latter often involves an iterative determination of induced point dipoles. The computational effort of the self-consistency procedure can be reduced by re-using...
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Creating new voices using normalizing flows
PublikacjaCreating realistic and natural-sounding synthetic speech remains a big challenge for voice identities unseen during training. As there is growing interest in synthesizing voices of new speakers, here we investigate the ability of normalizing flows in text-to-speech (TTS) and voice conversion (VC) modes to extrapolate from speakers observed during training to create unseen speaker identities. Firstly, we create an approach for TTS...
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Design specification management with automated decision-making for reliable optimization of miniaturized microwave components
PublikacjaThe employment of numerical optimization techniques for parameter tuning of microwave components has nowadays become a commonplace. In pursuit of reliability, it is most often carried out at the level of full-wave electromagnetic (EM) simulation models, incurring considerable computational expenses. In the case of miniaturized microstrip circuits, densely arranged layouts with strong cross-coupling effects make EM-driven tuning...
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The Most Valuable Global Brands and Condition of Economies: a Spatial Approach
PublikacjaResearch background: Brands are considered to be the most valuable asset of a company. Some of them achieve spectacular global results. The significance of global brands is proved by the fact that their value is often greater than the sum of all company’s net assets. Purpose of the article: The aim of this article is to highlight that brand value does not only create company’s value but also leverages economies. We claim that even though...
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Application of soil nailing technique for protection and preservation historical buildings
PublikacjaSoil nailing is one of the recent in situ techniques used for soil improvement and in stabilizing slopes. The process of soil nailing consists of reinforcing the natural ground with relatively small steel bars or metal rods, grouted in the pre-drilled holes. This method has a wide range of applications for stabilizing deep excavations and steep slopes. Soil nailing has recently become a very common method of slope stabilisation...
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Accelerated multi-objective design optimization of antennas by surrogate modeling and domain segmentation
PublikacjaMulti-objective optimization yields indispensable information about the best possible design trade-offs of an antenna structure, yet it is challenging if full-wave electromagnetic (EM) analysis is utilized for performance evaluation. The latter is a necessity for majority of contemporary antennas as it is the only way of achieving acceptable modeling accuracy. In this paper, a procedure for accelerated multi-objective design of...
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Local dynamics of fluids and dielectrics as the foundation of signal-carrying wave properties
PublikacjaThis paper develops an original approach to fundamental problems of classical linear acoustics and electromagnetics, proving a crucial role of doubly-dynamic local properties of a propagation medium in supporting wave-like fields able to carry information signals. The proof is composed of two steps concerning, subsequently, fluid acoustics and dielectric electromagnetics. The first step consists in complementing a common, practically...
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Surface active fatty acid ILs: Influence of the hydrophobic tail and/or theimidazolium hydroxyl functionalization on aggregates formation
PublikacjaNine structurally-related fatty acid ionic liquids have been prepared and their thermal behavior as well as their ability to self-assemble in water has been investigated. The thermal properties were studied by thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC), while the aggregation behavior was analyzed by tensiometry, isothermal titration calorimetry (ITC), conductometry, dynamic light scattering (DLS),...
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UV-induced electron transfer between triethylamine and 5-bromo-2′-deoxyuridine. A puzzle concerning the photochemical debromination of labeled DNA
Publikacja5-Bromo-2'-deoxyuridine (BrdU) photosensitizes DNA to strand break formation. However, this type of photodamage is completely quenched by the presence of triethylamine (TEA) which originates from RP-HPLC purification commonly employed by oligonucleotide providers. While the presence of TEA in oligonucleotide samples does not interfere with PCR or other molecular biology applications, the mechanism of photochemical reaction proceeding...
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Microextraction Techniques Used in the Procedures for Determining Organomercury and Organotin Compounds in Environmental Samples
PublikacjaDue to human activities, the concentrations of organometallic compounds in all parts of the environment have increased in recent decades. The toxicity and some biochemical properties of mercury and tin present in the environment depend on the concentration and chemical form of these two elements. The ever-increasing demand for determining compounds at very low concentration levels in samples with complex matrices requires...
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Homoisocitrate dehydrogenase from Candida albicans: properties, inhibition, and targeting by an antifungal pro-drug
PublikacjaThe LYS12 gene from Candida albicans, coding for homoisocitrate dehydrogenase was cloned and expressed as a His-tagged protein in Escherichia coli. The purified gene product catalyzes the Mg2+- and K+-dependent oxidative decarboxylation of homoisocitrate to alpha-ketoadipate. The recombinant enzyme demonstrates strict specificity for homoisocitrate. SDS-PAGE of CaHIcDH revealed its molecular mass of 42.6±1 kDa, while in size-exclusion...
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A Series-Inclined-Slot-Fed Circularly Polarized Antenna for 5G 28-GHz Applications
PublikacjaThis letter presents the design of a single-point-fed, geometrically simple circularly polarized (CP) antenna for 28 GHz Ka-band applications. The proposed antenna is based on a straight microstrip line printed on one side and coupled with the nearly square patches through a 45-degree inclined V-shape slot aperture on the other side. In order to generate circular polarization, the fundamental radiating mode is degenerated at a...
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Experimental investigation on heat pipes supported by soy wax and lauric acid for electronics cooling
PublikacjaThis paper presents an experimental study of heat pipes for electronics cooling that were supported by energy storage materials (i.e. phase change materials - PCMs) coated at the adiabatic section. The PCMs utilized included two materials, namely lauric acid and soy wax, the latter being considered as a more cost-effective alternative. The study focused on three different powers: 20 W, 25 W, and 30 W. Both heating, cooling, and...
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Hybridized PWM Strategy for Three- and Multiphase Three-Level NPC Inverters
PublikacjaA simple hybridized pulsewidth modulation (PWM) algorithm for three- and multiphase three-level neutral point clamped (NPC) inverters is proposed. The proposed solution is based on classical space vector PWM (SVPWM) algorithms for two-level inverters but can also be based on sinusoidal PWM with min–max injection. An additional level of output voltage is obtained by modifying the resulting switching patterns taking into account...
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Synchrotron radiation photoemission spectroscopy of the oxygen modified CrCl3 surface
PublikacjaWe investigate the experimentally challenging CrCl3 surface by photon energy dependent photoemission (PE). The core and valence electrons after cleavage of a single crystal, either in a ultrahigh vacuum (UHV) or in air, are studied by keeping the samples at 150 1C, aiming at confirming the atomic composition with respect to the expected bulk atomic structure. A common spectroscopic denominator revealed by data is the presence of...
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Rapid Simulation-Driven Multiobjective Design Optimization of Decomposable Compact Microwave Passives
PublikacjaIn this paper, a methodology for fast multiobjective optimization of the miniaturized microwave passives has been presented. Our approach is applicable to circuits that can be decomposed into individual cells [e.g., compact microstrip resonant cells (CMRCs)]. The structures are individually modeled using their corresponding equivalent circuits and aligned with their accurate, EM simulated...
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Mechatronic Design Towards Investigation of the Temporo-Mandibular Joint Behaviour
PublikacjaA significant problem of the temporo-mandibular joint (TMJ) research is lack of data concerning geometry and position of TMJ discs. It leads to necessity of developing a driving method of the process optimization, which is based on chosen techniques of mechatronic design. In particular, the latter concerns a technique of experimentally supported virtual prototyping. On this stage, the research is characterized by well-verified...
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Expedited Simulation-Driven Multi-Objective Design Optimization of Quasi-Isotropic Dielectric Resonator Antenna
PublikacjaMajority of practical engineering design problems require simultaneous handling of several criteria. Although many of design tasks can be turned into single-objective problems using sufficient formulations, in some situations, acquiring comprehensive knowledge about possible trade-offs between conflicting objectives may be necessary. This calls for multi-objective optimization that aims at identifying a set of alternative, Pareto-optimal...
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Uczelnia organizacją z domieszka turkusu - sznasa czy iluzja?
PublikacjaPo kilkuletnich dyskusjach nad przyszłym kształtem szkolnictwa wyższego w Polsce, zarówno na poziomie systemowym, jak i instytucjonalnym, w 2018 r. uchwalono ustawę Prawo o szkolnictwie wyższym i nauce, zwaną dalej Ustawą (2018). Regulacja ta wraz z pakietem rozporządzeń poszerzyła autonomię uczelni w zakresie organizacyjno-zarządczym, jednocześnie potęgując znaczenie ich rozliczalności. Wzmocnienie władzy rektora spowodowało zmianę...
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INVESTIGATION OF THE EFFECT OF THE ROCKET'S SUBORBITAL FLIGHT ON BIOFILM, ENZYMES AND BIOSYNTHESIS ON AUTONOMOUS, MODULAR AND SCALABLE PLATFORM FOR CONDUCTING EXPERIMENTS OF AN ASTROBIOTECHNOLOGICAL NATURE
PublikacjaWith new incentives for human space exploration, biotechnological experiments in orbit became imperative.Answering this need, we build an autonomous, modular, and scalable platform that enables those experiments onrockets. We called it AMBER. The aim of the payload of the R6 suborbital rocket is to perform an experiment tostudy the influence of rocket flights on biofilm, molecular...
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On adaptive covariance and spectrum estimation of locally stationary multivariate processes
PublikacjaWhen estimating the correlation/spectral structure of a locally stationary process, one has to make two important decisions. First, one should choose the so-called estimation bandwidth, inversely proportional to the effective width of the local analysis window, in the way that complies with the degree of signal nonstationarity. Too small bandwidth may result in an excessive estimation bias, while too large bandwidth may cause excessive...
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Explicit Size-Reduction of Circularly Polarized Antennas through Constrained Optimization with Penalty Factor Adjustment
PublikacjaModern communication systems of high data capacity incorporate circular polarization (CP) as the preferred antenna radiation field configuration. In many applications, integration of the system circuitry with antennas imposes size limitations on CP radiators, which makes their development process a challenging endeavor. This can be mitigated by means of simulation-driven design, specifically, constrained numerical optimization....
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The Effect of Conjugation with Octaarginine, a Cell-Penetrating Peptide on Antifungal Activity of Imidazoacridinone Derivative
PublikacjaAcridine cell-penetrating peptide conjugates are an extremely important family of compounds in antitumor chemotherapy. These conjugates are not so widely analysed in antimicrobial therapy, although bioactive peptides could be used as nanocarriers to smuggle antimicrobial compounds. An octaarginine conjugate of an imidazoacridinone derivative (Compound 1-R8) synthetized by us exhibited high antifungal activity against reference...