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Search results for: DOPING, IBUPROFEN, HORSE, URINE, GC-MS
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Simultaneous determination of ibuprofen and its metabolites in complex equine urine matrices by GC-EI-MS in excretion study in view of doping control
PublicationA novel assay for the simultaneous determination of ibuprofen (IBU) and its four probable metabolites, 1-hydroxyibuprofen (1-OH IBU), 2-hydroxyibuprofen (2-OH IBU), 3-hydroxyibuprofen (3-OH IBU) and carboxyibuprofen (CBX IBU) in equine urine samples with the application of Gas Chromatography-Electron Ionization-Mass Spectrometry (GC-EI-MS) has been developed and elaborated. The new approach for sample preparation including minimizing...
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Quantification of unconjugated and total ibuprofen and its metabolites in equine urine samples by gas chromatography–tandem mass spectrometry: Application to the excretion study
PublicationIbuprofen (IBU) is a widely used nonsteroidal anti-inflammatory drug (NSAID) in human and also veterinary medicine. NSAIDs are usually highly metabolized compounds in horses; they are often present in equine urine, mostly in their conjugated forms (glucuronides). Thus, hydrolysis to cleave the glucuronide linkage prior to anti-doping analysis is often necessary for improving detection. In this study, unconjugated and total IBU...
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Elimination of excretion of ibuprofen and its metabolites in horses
PublicationAnalytical procedure based on application of gas chromatography-mass spectrometry (GC-MS) at the step of detection, identification and quantitation of ibuprofen (IBU) and detection of its metabolites – 1-hydroxyibuprofen (1-OH IBU) and carboxyibuprofen (CBX IBU) in horse urine by selected ion monitoring mode after sample preparation covering extraction at pH 3-4 and methylation has been used. The ions at m/z 161, 220 (methylated...
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Recent Applications of 1D GC-MS and 2D GC-MS in Foodomics Studies
PublicationGas chromatography coupled with mass spectrometry and food chemistry have been tightly linked since the 1960s. This setup is also an integral part of the omics studies when it comes to low mass metabolites. Therefore, the marriage of GC-MS and foodomics is obvious. Two-dimensional gas chromatography coupled with mass spectrometry fits perfectly within the latest trends in foodomics, since it is tailored for holistic food analysis....
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PTR-MS and GC-MS as complementary techniques for analysis of volatiles: A tutorial review
PublicationThis tutorial review is a critical commentary on the combined use of two instrumental analytical techniques, namely GC-MS and PTR-MS. The first mention of such an analytical approach likely appeared after the year 2000 and despite many advantages, it has not been applied very often. Therefore, the aim of this article is to elaborate on the concept of their combined use and to provide a curse tutorial for those considering taking...
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A rapid and sensitive method for the quantitative analysis of ibuprofen and its metabolites in equine urine samples by gas chromatography-tandem mass spectrometry
PublicationIbuprofen is widely used in human and veterinary medicine for the treatment of chronic pain as well as rheumatic and musculoskeletal disorders. However, t he anal- gesic and anti-inflammatory properties of Ibuprofen have contributed to frequent drug abuse in equestr ian s ports. A sensitive and rapid gas chromatography with tandem mass spectrometry based method with a simple liquid-liquid extraction and deriva- tization requiring...
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CE-MS and GC-MS as “Green” and Complementary Methods for the Analysis of Biogenic Amines in Wine
PublicationTwo novel complementary analytical methods, namely an extraction-free capillary electrophoresis coupled to mass spectrometry (CE-MS) and direct immersion-solid-phase microextraction combined with gas chromatography-mass spectrometry (DI-SPME-GC-MS), have been developed and successfully applied for the determination of biogenic amines (BAs) in wine and fruit wine. They have been rigorously compared to each other in terms of various...
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Determination of odour concentration by TD-GC×GC–TOF-MS and field olfactometry techniques
PublicationField olfactometry is one of the sensory techniques used to determine odour concentration, in atmospheric air, directly in emission sources. A two-dimensional gas chromatography with time of flight mass spectrometer (GC×GC–TOF-MS) allows performing the chemical characterization of various groups of chemical compounds, even in complex mixtures. Application of these techniques enabled determination of odour concentration level in...
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Urine metabolic fingerprinting using LC–MS and GC–MS reveals metabolite changes in prostate cancer: A pilot study
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Application of GC-MS/MS technique for the determination of benzodiazepines in blood samples
PublicationAbstract Benzodiazepines (BZD) are widely used as pharmaceuticals in medicine. However, due to psychostimulatory effect of BZD, in the last decade, they are more and more often used as drugs of abuse. These compounds produce similar effects to classical illicit drugs and are sold as “legal” alternatives to them as new psychoactive substances (NPS). Currently new BZD, named designer benzodiazepines (DBZD), are synthesized each year...
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Comparison of GC–MS and MEKC methods for caffeine determination in preworkout supplements
PublicationIn this study, GC–MS- and MEKC-based methods for determination of caffeine (CAF) in preworkout supplements were developed and validated. The proposed protocols utilized minimal sample preparation (simple dilution and syringe filtration). The developed methods achieved satisfactory validation parameters, i.e. good linearity (R2 0.9988 and R2 0.9985 for GC–MS- and MEKC-based method, respectively), satisfactory intraand interaccuracy...
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GC-MS-based untargeted metabolomics of plasma and urine to evaluate metabolic changes in prostate cancer
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Problems of PAH quantification by GC-MS method using isotope-labelled standards.
PublicationChromatografia gazowa w połączeniu ze spektromerią mas (GC-MS) jest obecnie rutynowo stosowaną metodą w analityce środowiskowej do oznaczania śladowych ilości zanieczyszczeń organicznych. Stosowanie detektora mas sprawia, że wygodnym wzorcem wewnętrznym są stabilne izotopowo analogi oznaczanych związków. Związki te są wykorzystywane do śledzenia i kompensacji strat analitów podczas wykonywania określonych operacji związanych z...
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Development and validation of a GC–MS/MS method for the determination of 11 amphetamines and 34 synthetic cathinones in whole blood
PublicationPurpose Psychoactive compounds that contain a phenylethylamine structure (such as amphetamine-type stimulants and synthetic cathinones) are one of the major classes of stimulants on the recreational drug market. Approximately 670 new psychoactive substances (NPS) are monitored only in Europe; however, new psychoactive compounds are being developed for illicit trade each year. In this context, the development of new analytical procedures...
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Determination of benzodiazepines and Z-hypnotic drugs in whole blood samples by GC–MS/MS: Method development, validation and application
PublicationBenzodiazepines (BZDs) and Z-drugs are widely used as anxiolytics, sedative hypnotics, anticonvulsants and muscle relaxants. “Designer benzodiazepines” (DBZDs) are a new psychoactive substance class consisting of benzodiazepine derivatives that are not allowed for medical use and are known for being used recreationally. From a toxicologist standpoint, the huge number of such substances implicate a necessity for developing fast...
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A novel methodology for the enantiomeric resolution of methamphetamine, its precursors and intermediates by GC-MS
PublicationImpurity profiling and classification of abused drugs using chiral analytical techniques is of particular interest and importance because of the additional information obtained from this approach. When these methods are applied to the synthesis of illicitly used substances, they can supply valuable information about the conditions/chemicals used in the synthesis. In this study, simultaneous chiral separation of methylamphetamine,...
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Direct-Ultrasonic Assisted Microextraction Coupled with RTL-GC-FID/GC-MS as a Future Standard Procedure for Monitoring 26 potentially Allergenic Fragrances in Water Samples
PublicationThis research topic grew up as a result of awareness of constant anthropogenic and natural input to the environment constituents of personal care products (PCPs) residues. This group of emerging pollutants encompasses a wide range of chemicals, including potentially allergenic fragrance compounds. The aim of the present study was to investigate presence of 26 fragrance allergens in water samples. Simple and rapid methodology based...
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Monitoring of occupational exposure to volatile organohalogen solvents (VOXs) in human urine samples of dry-cleaner workers by TLHS-DAI-GC-ECD procedure
PublicationChlorinated hydrocarbon solvents are often used for dry-cleaning clothes in the laundry industry. The object of this study was to monitor the occupational exposure of dry-clean employees coming into contact with VOXs. 25 workers collected their urine samples before the work shift, after 4 hours of work and after the work shift. The analyses of urine samples and solvents used in dry-cleaning were performed using TLHS-DAI-GC-ECD....
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3-Monochloropropane-1,2-diol in Infant Foods and Human Breast Milk: Determination by GC-MS
Publication3-Monochloropropane-1,2-diol is one of the food borne contaminants called chloropropanols. Over the last decade plenty of analytical methods was developed in order to determine 3-monochloropropane-1,2-diol in its free and bound form in various foodstuffs with the use of GC-FID, GC-ECD, LC-TOFMS and finally GC-MS, which is now widely used in laboratories. The aim of this research was the determination of 3-monochloropropane--1,2-diol...
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Elucidation of transformation pathway of ketoprofen, ibuprofen, and furosemide in surface water and their occurrence in the aqueous environment using UHPLC-QTOF-MS
PublicationThe identification and determination of transformation products (TPs) of pharmaceuticals is essential nowadays, in order to track their fate in the aqueous environment and, thus, to estimate the actual pollution. However, this is a challenging task due to the necessity to apply high-resolution instruments enable to detect known and unknown compounds. This work presents the use of liquid chromatography quadrupole time-of-flight...
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Chiral analysis of chloro intermediates of methylamphetamine by one-dimensional and multidementional NMR and GC/MS
PublicationImpurity profiling and classification of abused drugs using chiral analytical techniques is of particular interest and importance because of the additional information obtained fromthis approach. When these methods are applied to the synthesis of illicitly used substances, they can supply valuable information about the conditions/chemicals used in the synthesis. We have applied GC and NMR methods to the study of intermediates found...
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Development and validation of GC–MS/MS method useful in diagnosing intestinal dysbiosis
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Characteristics of Cucumis metuliferus, Actinidia deliciosa and Musa paradisica fragrance profiles using a comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometric detection (GC×GC-TOF MS)
PublicationComprehensive two-dimensional gas chromatography with time-of-flight mass spectrometric detection (GC×GC-TOF-MS) is a modern analytical technique used in many fields. This technique enables an effective separation of volatile chemical compounds [1]. In recent years, the topic of healthy food and healthy living has become very popular. For this reason, more and more scientific publications related to the analysis of food products...
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The difficulties in polybrominated diphenyl ethers identification by GC-EI-MS technique
PublicationAwareness about the harmful effects of brominated flame retardants on living organisms increases mainly due to link their presence in the human environment with health disorders. Therefore is a need to conduct research aimed at content control of these chemicals in the environment. Recent improvements in injection techniques and mass spectrometer ionization methods have led to a variety of options to determine PBDEs in environmental...
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Miniaturized, green salting-out liquid–liquid microextraction coupled with GC–MS used to evaluate biogenic amines in wine samples
PublicationMonitoring of the biogenic amines (BAs) content in food products, including wine, is important due to the health and safety of consumers and from the quality control point of view. In a given study, simultaneous derivatization and salting-out liquid–liquid microextraction (SALLME) coupled with GC–MS for BAs determination from wine samples was developed. For the optimization the Box-Behnken design was applied, where three independent...
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Metodyka rozdzielania i oznaczania lotnych inhibitorów fermentacji w brzeczkach fermentacyjnych ciemnej fermentacji, techniką GC-MS
PublicationW pracy przedstawiono opracowaną w wyniku badań metodykę identyfikacji i oznaczania inhibitorów fermentacji w brzeczkach fermentacji ciemnej z biomasy ligno-celulozowej, z wykorzystaniem ekstrakcji ciecz-ciecz w sprzężeniu z chromatografią gazową ze spektrometrem mas (LLE-GC-MS). W ramach badań dokonano doboru korzystnych warunków ekstrakcji ciecz-ciecz, w tym: pH, objętości rozpuszczalnika ekstrakcyjnego, czasu ekstrakcji oraz warunków...
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Multi-residue method for the determination of 16 recently used pesticides from various chemical groups in aqueous samples by using DI - SPME coupled with GC-MS
PublicationA simple and solvent-free multi-residue method has been optimized to determine 16 currently used pesticides from different chemical groups in aqueous samples. The extraction of analytes was carried out with direct immersion solid-phase microextraction (DI-SPME) and for the identification and quantitative determination gas chromatography coupled with mass spectrometry (GC-MS) was applied. Two commonly used adsorbent coatings have...
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Identyfikacja lotnych składników ścieków z instalacji oksydacji asfaltów z wykorzystaniem chromatografii gazowej sprzężonej ze spektrometrią mas (GC-MS)
PublicationW pracy przedstawiono wyniki badań nad identyfikacją lotnych związków organicznych występujących w ściekach z instalacji oksydacji asfaltów. Ścieki poddano deemulgacji, w celu usunięcia fazy organicznej, a następnie ekstrakcji dichlorometanem, w celu wyizolowania pozostałych w ściekach związków organicznych. Zidentyfikowano 30 związków organicznych oraz szereg n-alkanów, które pozostały w ściekach pomimo zastosowanej deemulgacji....
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Problems of PAH quantification by GC–MS method using isotope-labelled standards
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Non-volatile molecular composition and discrimination of single grape white of chardonnay, riesling, sauvignon blanc and silvaner using untargeted GC–MS analysis
PublicationThis study developed and applied a GC–MS method aiming at molecular fingerprinting of 120 commercial single grape white wines (Chardonnay, Riesling, Sauvignon Blanc and Silvaner) for possible authentication according to grape variety. The method allowed detection of 372 peaks and tentative identification of 146 metabolites including alcohols, organic acids, esters, amino acids and sugars. The grape variety effect explained 8.3%...
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Modern approach for determination of lactulose, mannitol and sucrose in human urine using HPLC-MS/MS for the studies of intestinal and upper digestive tract permeability
PublicationA new analytical procedure was described for the simultaneous determination of lactulose, mannitol and sucrose in urine, in which HILIC chromatography and tandem mass spectrometry detection are used. Sugars are orally administered for the estimation of intestinal permeability in children digestive tract. Samples were purified by dispersive solid phase extraction (d-SPE) using Amberlite MB150 resin. Raffinose was selected as an...
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The chromatographic analysis of extracts from poplar (Populus sp.) - Laying program GC-MS
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Endogenous oxidative DNA base modifications analysed with repair enzymes and GC/MS technique
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Determination of free tyrosine in equestrian supplements by LC–MS/MS and comparison of its quantity with total free amino acids content in view of doping control
PublicationThe reports on the probable beneficial impact of tyrosine (TYR) supplementation on performance enhancement have contributed to the growth of interest in TYR in equestrian sports field and related industries, such as the manufacture of dietary and nutritional supplements. In this study, the first attempt to the assessment of horses exposure to TYR during nutritional supplementation was demonstrated by quantification of unbound TYR...
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Application of dynamic headspace and gas chromatography coupled to mass spectrometry (DHS-GC-MS) for the determination of oxygenated volatile organic compounds in refinery effluents
PublicationThe paper presents a new procedure for the determination of oxygenated volatile organic compounds (O-VOCs) in postoxidative effluents from the production of petroleum asphalt using dynamic headspace coupled to gas chromatography-mass spectrometry in the selected ion monitoring (SIM) mode (DHSGC-MS). Among the GC capillary columns tested, a polar SLB-IL111 column with the ionic liquid stationary phase was found to be superior due...
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OZNACZANIE POZOSTAŁOŚCI PESTYCYDÓW W ORGANIZMACH PSZCZELICH ORAZ W MIODZIE Z WYKORZYSTANIEM PROCEDURY OPARTEJ NA ZASTOSOWANIU CHROMATOGRAFÓW SPRZĘŻONYCH Z TANDEMOWYMI SPEKTROMETRAMI MAS (GC-MS/MS i LC-MS/MS)
PublicationPestycydy to bardzo liczna grupa różnorodnych związków chemicznych stosowa-nych w rolnictwie. Ze względu na swoją trwałość gromadzą się w różnych elementach środowiska i mogą występować w obszarze, na którym nigdy nie zostały użyte. Zachodzi także proces ich bioakumulacji w tkankach oraz narządach zwierząt i roślin. Stosowane w rolnictwie środki ochrony roślin (szczególnie w okresie wzmożonych zabiegów agro-technicznych wiosną...
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Validated GC–MS method for determination of bisphenol a and its five analogues in dietary and nutritional supplements
PublicationBisphenol A (BPA) and its analogues showing structural and functional similarity to BPA are commonly applied in various industrial applications and thus are becoming ubiquitous in the environment. At the same time there is increasing scientific evidence that exposure to these chemicals may lead to adverse health effects in human and wildlife. In recent years dietary and nutritional supplements dedicated for athletes have become...
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Identification of lipid fraction constituents from grasshopper (Chorthippus spp.) abdominal secretion with potential activity in wound healing with the use of GC–MS/MS technique
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GC-MS analysis, antidiabetic and antioxidant activity of methanolic extract of pluteus cervinus: an in vitro and in silico approach
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The application of isotopically labeled analogues for the determination of small organic compounds by GC/MS with selected ion monitoring
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Optimization of a novel procedure for determination of VOCs in water and human urine samples based on SBSE coupled with TD-GC-HRMS.
PublicationPodczas ozanczania lotnych związków chlorowcoorganicznych (t.j. bromodichlorometanu, dibromochlorometanu, bromoformu I tetrachloroetylenu) z próbek wody I moczu ludzkiego wykorzystano technikę ekstrakcji za pomocą ruchomego elementu sorpcyjnego typu Twister połączonego z desorpcją termiczną analitów do kolumny chromatografu gazowego wyposażonego w wysokosprawny spektrometr mas. Przeprowadzono optymalizację czasu (30 min) i prędkości...
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Metabolic Evaluation of Urine from Patients Diagnosed with High Grade (HG) Bladder Cancer by SPME-LC-MS Method
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Can matrix solid phase dispersion (MSPD) be more simplified? Application of solventless MSPD sample preparation method for GC–MS and GC–FID analysis of plant essential oil components
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Wykorzystanie technik GC×GC-TOF-MS i olfaktometrii terenowej do oceny uciążliwości zapachowej powietrza atmosferycznego na obszarach przyległych do terenu składowiska odpadów komunalnych
PublicationUciążliwość zapachowa jest poważnym problemem środowiskowym i jednym z powodów skarg ludności dotyczących złej jakości powietrza atmosferycznego. Źródłem emisji nieprzyjemnych zapachów do środowiska (odorów) są obiekty gospodarki komunalnej, w tym składowiska odpadów komunalnych. Ze względu na negatywny wpływ emisji substancji zapachowych na zdrowie i życie człowieka, konieczne jest stosowanie odpowiednich narzędzi analitycznych...
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The potential role of fatty acids in prostate cancer determined by GC–MS analysis of formalin-fixed paraffin-embedded tissue samples
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Method for the simultaneous determination of monoaromatic and polycyclic aromatic hydrocarbons in industrial effluents using dispersive liquid-liquid microextraction with GC-MS
PublicationWe present a new method for simultaneous determination of 22 monoaromatic and polycyclic aromatic hydrocarbons in postoxidative effluents from the production of petroleum bitumen using dispersive liquid-liquid microextraction coupled to gas chromatography and mass spectrometry. The eight extraction parameters including the type and volume of extraction and disperser solvent, pH, salting out effect, extraction and centrifugation...
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Determination of pharmaceutical residues in drinking water in Poland using a new SPE-GC-MS(SIM) method based on Speedisk extraction disks and DIMETRIS derivatization
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Application of Headspace Solid-phase Microextraction with Poly(1-vinyl-3- Hexylimidazolium Chloride) Polymeric Ionic Liquid Coating using GC - MS to Determine Volatile Fatty Acids in Dairy Wastewater
PublicationVolatile fatty acids were determined in this study using headspace solid-phase microextraction (HS-SPME) with a poly(1-vinyl-3-hexylimidazolium chloride) polymeric ionic liquid coated fiber followed by gas chromatography - mass spectrometry. Experimental parameters such as extraction time and temperature, sample volume as well as desorption time and temperature were optimized. Detection limits of the method were in the range of...
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Składowiska odpadów komunalnych jako źródła emisji zanieczyszczeń do środowiska
PublicationCelem pracy było przeprowadzenie badań mających na celu określenie związków uwalnianych do powietrza ze składowisk odpadów komunalnych oraz charakterystyka ich właściwości zapachowych i toksycznych. Próbki powietrza analizowano za pomocą techniki TD-GC-MS. Przeprowadzone badania pozwoliły na zidentyfikowanie następujących związków: styren, benzen, ksylen, toluen, etylobenzen, disiarczek dimetylu, dekan, kwas butanowy i octowy....
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Abuse or contamination? Ratio determination of clenbuterol enantiomers to distinguish between doping use and meat contamination
PublicationClenbuterol has been detected in urine of several athletes during routine doping control in the past years. Athletes claimed that the clenbuterol was ingested by eating contaminated meat. Providing analytical prove for this claim is difficult. clenbuterol consists of two enantiomers, R(-) and S(+)-clenbuterol. Currently available human and veterinary preparations were found to consist of a racemic mixture of both enantiomers. When...