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Search results for: recombinant strains
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On the plastic buckling of curved carbon nanotubes
PublicationThis research, for the first time, predicts theoretically static stability response of a curved carbon nanotube (CCNT) under an elastoplastic behavior with several boundary conditions. The CCNT is exposed to axial compressive loads. The equilibrium equations are extracted regarding the Euler–Bernoulli displacement field by means of the principle of minimizing total potential energy. The elastoplastic stress-strain is concerned...
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An enhanced method in predicting tensile behaviour of corroded thick steel plate specimens by using random field approach
PublicationThe present work investigates the possibility of using random field techniques in modelling the mechanical behaviour of corroded thick steel plate specimens. The nonlinear Finite Element method, employing the explicit dynamic solver, is used to analyse the mechanical properties of typical specimens. A material model considering full nonlinearity is used to evaluate the stress-strain response. The influence of major governing parameters...
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An experimental study on polymer mass
PublicationA new method of repairing damaged structures by filling the cracks with specially prepared polymer mass has been recently proposed. The technique is mainly dedicated to historical objects where minimum intervention is permitted. The flexible joint bonds the disrupted elements and ensures further safe exploitation of a damaged structure. The aim of the present paper is to show the results of an experimental study on polymer mass...
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Experimental investigations and damage growth modeling of EN‐AW 2024 aluminum alloy under LCF loading accounting creep pre‐deformation
PublicationThis article presents the results of experimental tests of creep rupture and of low-cycle fatigue (LCF) of EN-AW 2024 aluminum alloy devoid of damage and having preliminary damage. The preliminary damage was dealt in the process of creep at elevated temperature 100C, 200C, and 300C until achievement of two different strain values at each temperature. Samples with preliminary damage were subjected to fatigue tests at room temperature....
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The effect of elevated temperature on LCF damage growth in 2024AA – Experiment and modeling
PublicationThis paper presents the results of experimental low-cycle fatigue (LCF) tests conducted on EN-AW 2024 alloy in T3511 temper at 100 ◦C, 200 ◦C and 300 ◦C. The material’s basic fatigue characteristics were determined, such as fatigue life curve and cyclic strain curve. Both the hysteresis loop and nature of fracture surfaces obtained in macro- and microscopic observations (scanning electron microscope, SEM) were accounted for. The...
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Wybrane aspekty modelowania uderzenia pojazdem dźwigara wiaduktu
PublicationW pracy przedstawiono różne zagadnienia związane z uderzeniem pojazdu w dźwigar wiaduktu. Omówiono wybór modelu obliczeniowego, na podstawie porównania wyników dla modelu dźwigara zespolonego, modelu dźwigara stalowego oraz modelu wiaduktu. Następnie opisano model dźwigara zespolonego zastosowany w analizie MES oraz metodę wyznaczania współczynników tłumienia Rayleigha. Symulację numeryczną uderzenia dźwigara wiaduktu przez koparkę...
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Experimental verification and comparison of mode shape-based damage detection methods
PublicationThis paper presents experimental verification and comparison of damage detection methods based on changes in mode shapes such as: mode shape curvature (MSC), modal assurance criterion (MAC), strain energy (SE), modified Laplacian operator (MLO), generalized fractal dimension (GFD) and Wavelet Transform (WT).
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Scattering by parallel cylindrical posts with conducting strips. W: Pro- gress in electromagnetics research. Ed. J.A. Kong. Cambridge, Massachusett/ USA/: EMW Publ.**2003 s. 305-333, 18 rys. 5 tabl. bibliogr. 14 poz. Electromagnetic Waves. PIER [nr] 43. Rozpraszanie na równoległych metalizowanych obiektach cylindrycznych.
PublicationRozwinięto teorię rozpraszania fali elektromagnetycznej w obszarach otwar-tych i zamkniętych na metalizowanych obiektach cylindrycznych przy użyciuzmodyfikowanej procedury iteracyjnej i metody dopasowania rodzajów. W wynikuanalizy uzyskano pole rozproszone w przypadku struktur otwartych oraz odpo-wiedzi częstotliwościowe współczynników odbicia i transmisji w prostokąt-nych złączach falowodowych. Obrót i przemieszczenie...
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Cyclic Behavior of Masonry Shear Walls Retrofitted with Engineered Cementitious Composite and Pseudoelastic Shape Memory Alloy
PublicationThe behavior of masonry shear walls reinforced with pseudoelastic Ni–Ti shape memory alloy (SMA) strips and engineered cementitious composite (ECC) sheets is the main focus of this paper. The walls were subjected to quasi-static cyclic in-plane loads and evaluated by using Abaqus. Eight cases of strengthening of masonry walls were investigated. Three masonry walls were strengthened with different thicknesses of ECC sheets using...
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Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective.
PublicationThe RpoE sigma factor is essential for the viability of Escherichia coli. RpoE regulates extracytoplasmic functions including lipopolysaccharide (LPS) translocation and some of its non-stoichiometric modifications. Transcription of the rpoE gene is positively autoregulated by EσE and by unknown mechanisms that control the expression of its distally located promoter(s). Mapping of 5′ ends of rpoE mRNA identified five new transcriptional...
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Optical method supported by machine learning for urinary tract infection detection and urosepsis risk assessment
PublicationThe study presents an optical method supported by machine learning for discriminating urinary tract infections from an infection capable of causing urosepsis. The method comprises spectra of spectroscopy measurement of artificial urine samples with bacteria from solid cultures of clinical E. coli strains. To provide a reliable classification of results assistance of 27 algorithms was tested. We proved that is possible to obtain...
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Mechanizm współpracy z gruntem pali prefabrykowanych wbijanych w świetle próbnych obciążeń pali oprzyrządowanych
PublicationOpis badań i wyniki próbnych obciążeń statycznych dwóch pali prefabrykowanych wbijanych oprzyrządowanych w aparaturę do pomiaru rozkładu siły osiowej wzdłuż trzonu pala. Analiza wpływu czasu i procesu wbijania na charakterystykę osiadania pali prefabrykowanych wbijanych. Identyfikacja siły rezydualnej w palu prefabrykowanym wbijanym i jej wpływ na interpretację pomiarów ekstensometrycznych w próbnym obciążeniu pala. Wnioski na...
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Smart Email - Almost an Agent Platform
PublicationNetwork organizations suffer today of information overload and strain that rise their operational costs. One of the reasons of that is the dominance of email messaging as the princi-pal means of document exchange between their workers. Proac-tive documents can rationalize these costs and augment email systems with a process view based on collaboration patterns.
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Wizyjny system monitoringu stanu nakładek stykowych odbieraków prądu
PublicationEksploatowane do niedawna w Polsce nakładki pantografów kolejowych były wykonywane w postaci styków miedzianych. Na początku 2011 r. przeprowadzono kompleksową zmianę typu nakładek stykowych, stosowanych przez operatorów korzystających z infrastruktury PKP PLK S.A., z miedzianych na metalizowane węglowe. Wraz z wprowadzeniem nakładek węglowych istnieje potrzeba opracowania nowych metod diagnostyki tego istotnego elementu toru zasilania...
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A novel cold-active β-D-galactosidase with transglycosylation activity from the Antarctic Arthrobacter sp. 32cB - gene cloning, purification and characterization
PublicationA gene encoding a novel β-D-galactosidase from the psychrotolerant Antarctic bacterium Arthrobacter sp. 32cB was isolated, cloned and expressed in Escherichia coli. The active form of recombinant β-D-galactosidase consists of two subunits with a combined molecular weight of approximately 257 kDa. The enzyme's maximum activity towards o-nitrophenyl-β-D-galactopyranoside was determined as occurring at 28 °C and pH 8.0. However, it...
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Immobilization on magnetic nanoparticles of therecombinant trehalose synthase from Deinococcusgeothermalis
PublicationtIn our study the gene encoding trehalose synthase from Deinococcus geothermalis was cloned and overexpressed inEscherichia coli Rosetta (DE3)pLysS. Wild-type trehalose synthase has been purified from host protein after cell dis-ruption and precipitation at 20% ammonium sulphate saturation. Recombinant trehalose synthase was immobilizedonto glutaraldehyde activated silanized magnetic ferrous-ferric oxide by using covalent binding...
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Bee Bread Exhibits Higher Antimicrobial Potential Compared to Bee Pollen
PublicationThis study aimed at investigation of the antimicrobial potential of ethanolic extracts of bee bread (BB) and bee pollen (BP) and suspensions of these products in MHB (Mueller Hinton Broth). We covered 30 samples of BP and 19 samples of BB harvested in Polish apiaries. Slightly lower activity was observed against Gram-negative bacteria compared to Gram-positive staphylococci. BB extracts exhibited higher inhibitory potential with...
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Characterisation of Escherichia coli isolates from the blood of haematological adult patients with bacteraemia: translocation from gut to blood requires the cooperation of multiple virulence factors
PublicationThe aim of the study was to investigate whether there are unique pathotypes of Escherichia coli capable of transmission from the gastrointestinal tract to the vascular bed. The study included E. coli strains isolated from clinical materials collected from 115 patients suffering from haematologic malignancies diagnosed with bacteraemia. The genotyping techniques established that 89 E. coli isolates from the blood had the same genotype...
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Novel staphylococci nucH taxonomical marker used in identification of human-associated Staphylococcus succinus subsp. casei
PublicationThe aim of our study was to assess the sequencing of unique nucH gene fragment based on performed bioinformatics analysis as a novel diagnostic method for the identification of difficult to identify staphylococcal human pathogenic strains. Initially, PCR-RFLP-rrn analysis specific to the spacers between 16SrDNA and 23SrDNA followed by HhaI restriction analysis was performed. Further, sequencing of nucH and 16S rDNA genes fragments...
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Example of tension fabric structure analysis
PublicationThe aim of the work is to examine two variants of non-linear strain-stress relations accepted to description of architectural fabric. Discussion on the fundamental equations of the dense net model, used in description of coated woven fabric behaviour is presented. An analysis of tensile fabric structures subjected to the dead load and initial pretension is described.
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Next Generation Digital
PublicationThe paper outlines the major objectives of the MENAID research project, eimed at novel architectures of digital documents. Such documents will enable reduction of information overflow and strain, a major threat to the growth of a digital society. They will be forward compatible, technology neutral and lightweight, allowing workers of network organizations to use personal devices of any type.
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Naturally occurring mitochondrial DNA heteroplasmy in the MRL mouse
PublicationThe MRL/MpJ mouse is an inbred laboratory strain of Mus musculus, known to exhibit enhanced autoimmunity, increased wound healing, and increased regeneration properties. We report the full-length mitochondrial DNA (mtDNA) sequence of the MRL mouse (Accession # EU450583), and characterize the discovery of two naturally occurring heteroplasmic sites. The first is a T3900C substitution in the TpsiCloop of the tRNA methionine gene...
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Surface and interfacial anti-plane waves in micropolar solids with surface energy
PublicationIn this work, the propagation behaviour of a surface wave in a micropolar elastic half-space with surface strain and kinetic energies localized at the surface and the propagation behaviour of an interfacial anti-plane wave between two micropolar elastic half-spaces with interfacial strain and kinetic energies localized at the interface have been studied. The Gurtin–Murdoch model has been adopted for surface and interfacial elasticity....
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An isogeometric finite element formulation for geometrically exact Timoshenko beams with extensible directors
PublicationAn isogeometric finite element formulation for geometrically and materially nonlinear Timoshenko beams is presented, which incorporates in-plane deformation of the cross-section described by two extensible director vectors. Since those directors belong to the space R3, a configuration can be additively updated. The developed formulation allows direct application of nonlinear three-dimensional constitutive equations without zero...
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Hydrogen degradation of pre-oxidized zirconium alloys
PublicationThe presence of the oxide layers on Zr alloys may retard or enhance the hydrogen entry and material degradation, depending on the layer features. This research has been aimed to determine the effects of pre-oxidation of the Zircaloy-2 alloy at a different temperature on hydrogen degradation. The specimens were oxidised in laboratory air at 350°C, 700°C, and 900°C. After, some samples were tensed at 10-5 strain rate and simultaneously...
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Static and dynamic concrete calculations: Breakable aggregates in DEM model
PublicationThe paper deals with the calculations of a 3-point bending beam under static and dynamic loads. The real microstructure was obtained from laboratory tests using micro-tomography images. The quasi-static results were compared directly with experimental data at both macro and micro levels. Subsequently, higher strain rates were applied to investigate dynamic effects. The study focused on the influence of dynamic loading on the macroscopic...
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Modelling of shear zones during quasi-static granular silo flow using material point method (MPM)
PublicationThe paper focuses on confined silo flow of cohesionless sand. The problem considered is a quasi-static flow in a plane strain model silo with parallel walls simulated with the material point method (MPM). The simulation used a non-local hypoplastic constitutive model. Initially, the paper validated the implemented numerical approach with basic element tests and a plane strain compression test. The subsequent MPM calculations for...
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DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation
PublicationA new diagnostic kit for K. oxytoca specific differentiation based on ddLMS PCR (ang. double digest ligation Mediated PCR) technique is shown. As a species-specific DNA fragment pehX gene, encoding the enzyme polygalactouronase, was chosen. The genome sequence of K. oxytoca is digested with two endonucleases: AclI and BclI which cut DNA before and after pehX gene. The polymorphic DNA fragments are ligated with AclI-end-specific...
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Bacterial nanocellulose as a microbiological derived nanomaterial
PublicationBacterial nanocellulose (BNC) is a nanofibrilar polymer produced by strains such as Gluconacetobacter xylinus, one of the best bacterial species which given the highest efficiency in cellulose production. Bacterial cellulose is a biomaterial having unique properties such as: chemical purity, good mechanical strength, high flexibility, high absorbency, possibility of forming any shape and size and many others. Such a large number...
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Wybrane zagadnienia optymalizacji organizacji ruchu kolejowego w celu minimalizacji kosztów energii elektrycznej
PublicationW artykule przedstawiono podział kosztów w transporcie kolejowym z uwzględnieniem kosztów wewnętrznych przedsiębiorstwa, do których zaliczają się między innymi koszty dostępu do infrastruktury, czy koszty energii. Stwierdzono, że przy odpowiedniej organizacji ruchu pociągów na sieci kolejowej, bez ponoszenia dodatkowych nakładów na infrastrukturę i specjalistyczne urządzenia, można znacznie ograniczyć zużycie energii, a co za tym...
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Laser granulometer as an useful tool for selection of appropriate membranes used in the MIEX®DOC-UF/MF hybrid process
PublicationIn the study, particle size distribution of the MIEX® resin was presented. Such analyses enable to determinate whether presence of fine resin fraction may be the reason for unfavorable membrane blocking during water purification by the hybrid MIEX®DOC-microfiltration/ultrafiltration systems. Granulometric analysis of resin grains using the laser diffraction particle size analyzer (laser granulometer) was carried out as well as...
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In vitro enzyme kinetics and NMR-based product elucidation for glutathione S-conjugation of the anticancer unsymmetrical bisacridine C-2028 in liver microsomes and cytosol: major role of glutathione S-transferase M1-1 isoenzyme
PublicationThis work is the next step in studying the interplay between C-2028 (anticancer-active unsymmetrical bisacridine developed in our group) and the glutathione S-transferase/glutathione (GST/GSH) system. Here, we analyzed the concentration- and pH-dependent GSH conjugation of C-2028 in rat liver microsomes and cytosol. We also applied three recombinant human GST isoenzymes, which altered expression was found in various tumors. The...
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Numerical modelling of the mesofracture process of sintered 316L steel under tension using microtomography
PublicationThis paper concerns numerical modelling of the deformation process, taking into account the local fracture of porous 316L sinters at the mesoscopic scale using the finite element method. Calculations are performed with the use of geometrical models, to map the realistic shape of the porous mesostructure of the material, obtained by means of computed microtomography. The microtomographic device has limited and insufficient measurement...
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Examination of advanced isotropic constitutive laws under complex stress states in plain and reinforced concrete specimens
PublicationThe performance of advanced isotropic constitutive laws under complex stress states in plain and reinforced concrete specimens is investigated. Three different formulations are chosen: original Mazars model, Mazars mi model and model proposed by Pereira and coworkers. The degradation of the material in all formulations is described via a single variable, but a strain/stress state is taken into account via quite sophisticated relationships....
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A brief note on entire fracture surface topography parameters for 18Ni300 maraging steel produced by LB-PBF after LCF
PublicationThe concept of entire fracture surface investigation is helpful in explaining fatigue phenomena. In this paper, this method has been applied for 18Ni300 maraging steel using a 3D measurement system. Before post-mortem analysis, the specimens produced by laser beam powder bed fusion (LB-PBF) were tested under low-cycle fatigue (LCF) for eight strain amplitudes in the interval 0.3% to 1.0%. The attention was placed on the relationship...
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On a 3D material modelling of smart nanocomposite structures
PublicationSmart composites (SCs) are utilized in electro-mechanical systems such as actuators and energy harvesters. Typically, thin-walled components such as beams, plates, and shells are employed as structural elements to achieve the mechanical behavior desired in these composites. SCs exhibit various advanced properties, ranging from lower order phenomena like piezoelectricity and piezomagneticity, to higher order effects including flexoelectricity...
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On the effective properties of foams in the framework of the couple stress theory
PublicationIn the framework of the couple stress theory, we discuss the effective elastic properties of a metal open-cell foam. In this theory, we have the couple stress tensor, but the microrotations are fully described by displacements. To this end, we performed calculations for a representative volume element which give the matrices of elastic moduli relating stress and stress tensors with strain and microcurvature tensors.
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Free Vibration of Flexomagnetic Nanostructured Tubes Based on Stress-driven Nonlocal Elasticity
PublicationA framework for the flexomagneticity influence is here considered extending the studies about this aspect on the small scale actuators. The developed model accommodates and composes linear Lagrangian strains, Euler-Bernoulli beam approach as well as an extended case of Hamilton’s principle. The nanostructured tube should subsume and incorporate size effect; however, for the sake of avoiding the staggering costs of experiments,...
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Fracture prediction in flat PMMA notched specimens under tension - effectiveness of the equivalent material concept and fictitious material concept
PublicationThe fracture of notched elements under mode I loading (tension) remains an inexhaustible research topic, especially when it comes to the fracture of thermoplastic materials such as polymethylmethacrylate (PMMA), which experience considerable plastic strains under tension. The paper points out that traditional brittle fracture criteria such as mean stress (MS) or maximum tangential stress (MTS) criteria used to predict this phenomenon do...
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M-integral for finite anti-plane shear of a nonlinear elastic matrix with rigid inclusions
PublicationThe path-independent M-integral plays an important role in analysis of solids with inhomogeneities. However, the available applications are almost limited to linear-elastic or physically non-linear power law type materials under the assumption of infinitesimal strains. In this paper we formulate the M-integral for a class of hyperelastic solids undergoing finite anti-plane shear deformation. As an application we consider the problem...
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The occurrence of heavy metals and metal-resistant bacteria in water and bottom sediments of the Straszyn reservoir (Poland)
PublicationThe aim of this study is investigate the distribution of selected heavy metals (zinc, copper, lead, cadmium) and metal-resistant bacteria in water and bottom sediments of drinking water reservoir for Gdańsk (Poland). The research area - Straszyn Lake is a dam reservoir on Radunia river, of the total volume of 3 mln m3. Sediment and water sampling took place between May and October 2013 (eight series). Regarding metal concentration...
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Genetic and biochemical characterization of yeasts isolated from Antarctic soil samples
PublicationThe Polish Arctowski Station is situated in the maritime Antarctic on the western shore ot' Admiralty Bay and encompasses terrestrial habitats which are not perma-nently covered by ice, in contrast to morę than 90% of the island's surface area. Over the past several decades, stud-ies exploring the soils of those habitats have revealed a considerable diversity of bacteria, filamentous fungi, and, to a lesser extent, yeasts; however,...
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Badania eksperymentalne strefy przypodporowej żelbetowych belek częściowo sprężonych
PublicationThis project refers to the study the shear zone, under full and partial prestressing in comparison to beams with passive reinforcement.For comparison, variable parameters have been determined:- a/d (from 1,5 to 3,5) - it's shear span to effective depth of the beam . Assuming constant geometrical quantities, "a" is variable.- 26 beams had no vertical shear reinforcement, and the other 26 had closed stirrups.- prestressing intensity...
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Effect of Double-Slope Modulation Signals on Conducted Emissions and Efficiency of Strongly Coupled Magnetic Resonance WPT Systems
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Effect of exercise training on insulin sensitivity, mitochondria and computed tomography muscle attenuation in overweight women with and without polycystic ovary syndrome
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Research and Development of Inductive-Resonant Wireless Power Transfer System
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Effect of Hybrid Modulation on Performance of Wireless Battery Charger Operating in CC/CV Mode
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Effect of surface on the flexomagnetic response of ferroic composite nanostructures; nonlinear bending analysis
PublicationOur analysis incorporates the geometrically nonlinear bending of the Euler-Bernoulli ferromagnetic nanobeam accounting for a size-dependent model through assuming surface effects. In the framework of the flexomagnetic phenomenon, the large deflections are investigated referring to von-Kármán nonlinearity. Employing the nonlocal effects of stress coupled to the gradient of strain generates a scale-dependent Hookean stress-strain...
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On the crack front curvature in bonded joints
PublicationStandard tests of adhesively bonded specimens are likely to produce heterogeneous stress distribution along the crack front and its vicinity. High separation rate mode I dominated fracture test is performed.Observation of post mortem fractured surfaces with an optical microscope reveals characteristic features of mixed mode I/III fracture near the sides of the specimen but not in the middle. At first, finite elements calculations...
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Numerical analyses of novel prefabricated structural wall panels in residential buildings based on laboratory tests in scale 1:1
PublicationThe paper presents experimental and numerical investigations on novel prefabricated composite building wall panels for residential building constructions. The wall panels were composed of reinforced concrete ribbed elements with the core from the EPS foam as the thermal insulation. The wall panels in the full-scale 1:1 were subjected to vertical loads. In the first step, the experiments were analysed with simple usual static methods....