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Search results for: CANDIDA KRUSEI
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alfa-Aminotransferaza kwasu L-alfa-aminoadypinowego z Candida albicans jako cel molekularny
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New PCR test for detection of Candida glabrata based on the molecular target chosen by the RAPD technique
PublicationRapid, reliable diagnosis is a necessary condition for the successful treatment of infections. Such diagnostic assays are continually being developed. #e paper presents a method for selecting the molecular target for PCR-based diagnostics based on the comparison of RAPD patterns. A sequence encoding Candida glabrata CBS138 hypothetical protein was selected. The limit of detection for PCR and real-time PCR reactions with DNA extracted...
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Evaluation of possibilities of genotyping of Candida glabrata clinical isolates with RAPD-PCR method and microsatellite analysis
PublicationThe aim of the study was to compare the discriminatory power of RAPD-PCR method using RSD10 primer and microsatellite (GACA)4 analysis for genotyping clinical isolates of C. glabrata. Isolates were received from patients of two Polish hospitals: Children's Memorial Health Institute in Warsaw (n=17) and Medical University of Gdansk (n=37). Species identification was confirmed by two phenotypic methods: growth on chromagar plates...
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Phosphorylation of glucosamine-6-phosphate synthase is important but not essential for germination and mycelial growth of Candida albicans.
PublicationStosując mutagenezę ukierunkowaną dla genu gfa1 kodującego syntazę glukozamino-6-fosforanu Candida albicans otrzymano zmutowaną wersję GFA1S208A. Produkt zmutowanego genu, który utracił miejsce fosforylacji dla kinazy A, wykazywał wszystkie właściwości identyczne do dzikiego enzymu, jednakże nie był substratem dla kinazy. Brak fosforylacji syntazy glukozamino-6-fosforanu przez kinazęA prowadził do redukcji lecz nie eliminował...
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ABC transporters Cdr1p, Cdr2p and Cdr3p of a human pathogen Candida albicans are general phospholipid translocators
PublicationFluoryzujące analogi fosfolipidów NBD-PE, NBD-PC i NBD-PS zostały wykorzystane w celu wykazania, że białko Cdr1p i jego inne homologi, Cdr2p i Cdr3p, należące do grupy białek zawierających tzw. kasetę wiążącą ATP (ABC), zachowują się jak ogólne przenośniki lipidów. Wyniki badań pokazują, że transportery typu ABC występujące w C. albicans są przenośnikami fosfolipidów i aktywność ta może być jedna z fizjologicznych funkcji białek.
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Systematic comparison of a biotrickling filter and a conventional filter for the removal of a mixture of hydrophobic VOCs by Candida subhashii
PublicationThis work systematically compared the potential of a conventional fungal biofilter (BF) and a fungal biotrickling filter (BTF) for the abatement of a mixture of hydrophobic volatile organic compounds (VOCs). Candida subhashii was herein used for the first time, to the best of the author's knowledge, to remove n-hexane, trichloroethylene, toluene and α-pinene under aerobic conditions. C. subhashii immobilized on polyurethane foam...
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Regulacja aktywności syntazy glukozamino-6-fosforanu z Candida albicans – badania metodami obliczeniowymi
PublicationNiniejsza praca przedstawia wyniki badań, prowadzonych metodami obliczeniowymi, poświęconych enzymowi syntazie GlcN-6-P pochodzącemu z drożdży C. albicans. Enzym ten odpowiedzialny jest między innymi za biosyntezę kluczowych składników ściany komórkowej grzybów i bakterii, ma również wpływ na regulację metabolizmu glukozy. Przedstawione badania stanowią jeden z etapów poszukiwania molekularnego mechanizmu inhibicji, której eukariotyczna...
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The Antimicrobial Activity of Omiganan Alone and In Combination against Candida Isolated from Vulvovaginal Candidiasis and Bloodstream Infections
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Inhibitory Effect of Eugenol and trans‐Anethole Alone and in Combination with Antifungal Medicines on Candida albicans Clinical Isolates
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Photodynamic Inactivation of Candida albicans with Imidazoacridinones: Influence of Irradiance, Photosensitizer Uptake and Reactive Oxygen Species Generation
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Możliwości zastosowania metody Kansei Engineering w projektowaniu usług on-line dla osób zagrożonych wykluczeniem cyfrowym
PublicationW artykule przedstawiono problematykę projektowania usług on-line dla osób zagrożonych wykluczeniem cyfrowym. Zaprezentowano ogólne zalecenia oraz praktyczne zasady dotyczące ich projektowania. Wymieniono główne czynniki wpływające na efektywność wykorzystywania interaktywnych form współpracy z różnymi grupami użytkowników. Jednocześnie wskazano na możliwość przeciwdziałania problemowi wykluczenia cyfrowego poprzez zastosowanie...
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Bacteriophage–Ciprofloxacin Combination Effectiveness Depends on Staphylococcus aureus–Candida albicans Dual-Species Communities’ Growth Model
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Evaluation of possibilities in identification and susceptibility testing for Candida glabrata clinical isolates with the Integral System Yeast Plus (ISYP)
PublicationThe aim of this study was to evaluate possibilities of correct identification and susceptibility testing of C. glabrata clinical isolates with Integral System Yeast Plus (ISYP). For species identification, as the reference method, API Candida test and species-specific PCR reactions were used. The potential of antifungal susceptibility testing by the ISYP test was compared with the Sensititre Yeast One. Whilst the reference methods...
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A novel in vitro assay for assessing efficacy and toxicity of antifungals using human leukaemic cells infected with Candida albicans
PublicationAims: This study describes a novel in vitro assay that simultaneously determines antifungal efficiency and host cell toxicity using suspensions of human leukaemic cells (HL-60) infected with Candida albicans. Methods and Results: The effect of Candida infection on host cell viability was evaluated by the microscopy of trypan blue-stained cells and lactate dehydrogenase (LDH) activity. The in vitro ‘drug potency assay’ utilized...
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Crystallization and preliminary X-ray analysis of the isomerase domain of glucosamine-6-phosphate synthase from Candida albicans
PublicationSyntaza glukozamino-6-fosforanu (EC 2.6.1.16) katalizuje pierwszy, praktycznie nieodwracalny etap w szlaku biosyntezy heksozoamin, którego produktem końcowym jest UDP-GlcNAc, kluczowy substrat dla tworzenia ściany komórkowej drobnoustrojów. Otrzymano kryształy domeny izomerazowej syntazy GlcN-6-P z Candida albicans, składającej się z reszt 346-712 (42 kDa). Analiza rentgenograficzna kryształu wykazała, że należy on do grupy przestrzennej...
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Oporność patogennych drożdżaków z rodzaju Candida na chemoterapeutyki przeciwgrzybowe i nowe sposoby jej przełamywania
PublicationCelem badania było określenie podatności na leki przeciwgrzybowe izolatów Candida od pacjentów z polskich szpitali oraz określenie mechanizmów oporności na nie. Drugim celem było zbadanie aktywności przeciwgrzybowej produktów naturalnych (olejków eterycznych i propolisów pszczelich) i mechanizmów ich działania. Prawie 20% zebranych izolatów wykazało oporność na flukonazol oraz oporność krzyżową na cztery badane azole. Większość...
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Virulence, antifungal susceptibility and molecular mechanisms of echinocandin resistance among Candida isolates recovered from clinical specimens
PublicationFungi of the genus Candida belong to the natural microflora of healthy individuals. However, they can also be a cause of opportunistic infections especially among patients with an impaired immune system. The first line therapy of Candida infections is based on triazoles. However, in recent years there an increase of azole resistant Candida spp., in particular C. glabrata and C. krusei, has been observed. For this reason, echinocandin...
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Hydrazinolysis Products of Selected Sugar Lactones—Crystal Structure and Microbiological Activity
PublicationCommercially available lactones, as well as those synthesized by us, turned out to be good substrates for the synthesis of sugar hydrazides. The exception was L-ascorbic acid, whose hydrazinolysis led to the formation of a hydrazinium salt, not the hydrazide as expected. The structure of all compounds was confirmed by NMR and X-ray analyses. The lower durability of hydrazinium L-ascorbate was additionally confirmed by thermogravimetric...
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Characterization of the interactions between human high-molecular-mass kininogen and cell wall proteins of pathogenic yeasts, Candida tropicalis
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MOŻLIWOŚCI WYKORZYSTANIA OLEJKÓW ETERYCZNYCH W PROFILAKTYCE I TERAPII INFEKCJI WYWOŁYWANYCH PRZEZ DROŻDŻE Z RODZAJU CANDIDA
PublicationW artykule przedstawiono perspektywy wykorzystania olejków eterycznych w terapii i profilaktyce chorób infekcyjnych wywoływanych przez drożdże. Wyniki badań z ostatnich lat jednoznacznie potwierdziły, że olejki izolowane z wielu roślin wykazują znaczną aktywność przeciwgrzybową. W przypadku infekcji powierzchniowych mogłyby one stanowić cenną alternatywę, bądź czynnik wspomagający dla stosowanych aktualnie chemioterapeutyków przeciwgrzybowych....
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A new assay based on terminal restriction fragment length polymorphism of homocitrate synthase gene fragments for Candida species identification
PublicationCandida sp. have been responsible for an increasing number of infections, especially in patients with immunodeficiency. Species specific differentiation of the Candida sp. is difficult in routine diagnosis. This identification can have a highly significant association in therapy and prophylaxis. This work has shown a new application of terminal restriction fragment length polymorphism (t-RFLP) method in molecular identification...
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Construction, purification, and functional characterization of His-tagged Candida albicans glucosamine-6-phosphate synthase expressed in Escherichia coli
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Oxolane Ammonium Salts (Muscarine-Like)—Synthesis and Microbiological Activity
PublicationCommercially available 2-deoxy-D-ribose was used to synthesize the appropriate oxolane derivative—(2R,3S)-2-(hydroxymethyl)oxolan-3-ol—by reduction and dehydration/cyclization in an acidic aqueous solution. Its monotosyl derivative, as a result of the quaternization reaction, allowed us to obtain eight new muscarine-type derivatives containing a quaternary nitrogen atom and a hydroxyl group linked to the oxolane ring. Their structure...
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Prevalence of Closely Related Candida albicans Species among Patients with Vulvovaginal Candidiasis in Southern Poland Based on the hwp1 Gene Amplification
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Novel application of terminal restriction fragments length polymorphism method (t-RFLP) for identification of six clinically important Candida sp.
PublicationTerminal restriction fragments length polymorphism method (t-RFLP) is a modified classical RFLP method. The main difference is based on carrying out PCR using a fluorescently labeled primer or primers. The result of such modification is that after digestion of amplicons only terminal fragments of PCR product (containing fluorescent dye) are visualized. T-RFLP method has found application in environmental microbiology and epidemiology....
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Esplanade Riel footbridge, Winnipeg, Canada - a study
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Modification of quaternary structure of Candida albicans GlcN-6-P synthase and its desensitization to inhibition by UDP-GlcNAc by site-directed mutagenesis
PublicationSite-directed mutagenesis of the CaGFA1 gene encoding glucosamine-6-phosphate synthase from Candida albicans was performed. Desensitization of the enzyme to inhibition by UDPGlcNAc was achieved upon T487I and H492F substitutions at the UDP-GlcNAc binding site, exchange of D524, S525 and S527 for Ala at the dimer:dimer interface and construction of the tail-lock array (L434R and L460A) at the C-tail region. The first two sets if...
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Emerging anticancer activity of candidal glucoseamine-6-phosphate synthase inhibitors upon nanoparticle-mediated delivery
PublicationNumerous glutamine analogues have been reported as irreversible inhibitors of the glucosamine-6-phosphate (GlcN-6-P) synthase in pathogenic Candida albicans in the last 3.5 decades. Among the reported inhibitors, the most effective N3-(4-methoxyfumaroyl)-L-2,3- diaminopropanoic acid (FMDP) has been extensively studied in order to develop its more active analogues. Several peptide−FMDP conjugates were tested to deliver FMDP to its...
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A Comparative Study on the Toxicity of Chlorides and Tri-tert-butoxysilanethiolates of Zinc(II), Cadmium(II) and Mercury(II) Towards Candida albicans and Escherichia coli.
PublicationMetale przejściowe, czyli cynk, kadm i rtęć należą do XII grupy układu okresowego. Cynk jako element strukturalny białek odgrywa zróżnicowaną rolę biologiczną i jest pierwiastkiem niezbędnym do zachowania funkcji życiowych. W odróżnieniu od niego kadm i rtęć są metalami wykazującymi działanie toksyczne i podejrzanymi o działanie rakotwórcze. Kadm w szkodliwy sposób wpływa na uprawy oraz organizm ludzki. Toksyczne działanie rtęci...
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Enzymy o aktywności aminotransferazy kwasu L-α-aminoadypinowego z Candida albicans - właściwości i rola w metabolizmie komórki grzybowej
PublicationCandida albicans jest elementem naturalnej flory drobnoustrojowej człowieka. Podobieństwo komórek grzybowych i ssaczych, wzrost częstości występowania infekcji grzybiczych wraz ze stopniowym wzrostem pojawiania się oporności patogenów stwarza potrzebę poszukiwania nowych związków przeciwgrzybowych. Jedną z dróg obraną przez naukowców jest analiza szlaków biosyntezy aminokwasów egzogennych dla człowieka. Przedmiotem badań w ramach...
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Correction to “Emerging Anticancer Activity of Candidal Glucosamine-6-Phosphate Synthase Inhibitors upon Nanoparticle-Mediated Delivery”
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Fluconazole resistant Candida auris clinical isolates have increased levels of cell wall chitin and increased susceptibility to a glucosamine-6-phosphate synthase inhibitor
PublicationIn 2009 Candida auris was first isolated as fungal pathogen of human disease from ear canal of a patient in Japan. In less than a decade, this pathogen has rapidly spread around the world and has now become a major health challenge that is of particular concern because many strains are resistant to multiple class of antifungal drugs. The lack of available antifungals and rapid increase of this fungal pathogen provides an incentive...
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Method-Dependent Epidemiological Cutoff Values for Detection of Triazole Resistance in Candida and Aspergillus Species for the Sensititre YeastOne Colorimetric Broth and Etest Agar Diffusion Methods
PublicationAlthough the Sensititre Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candida or Aspergillus species. We collected fluconazole, itraconazole, posaconazole, and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method/agent-dependent) 11,171 Candida albicans, 215 C. dubliniensis, 4,418 C. glabrata species complex,...
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Evaluations of Men in Domestic Roles in Canada, Norway, Poland, and India
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Molecular Umbrella as A Nanocarrier for Antifungals
PublicationA molecular umbrella composed of two O‐sulfated cholic acid residues was applied for the construction of conjugates with cispentacin, containing a “trimethyl lock” (TML) or o‐dithiobenzylcarbamoyl moiety as a cleavable linker. Three out of five conjugates demonstrated antifungal in vitro activity against C. albicans and C. glabrata but not against C. krusei, with MIC90 values in the 0.22–0.99 mM range and were not hemolytic. Antifungal...
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Circular Fashion – Consumers’ Attitudes in Cross-National Study: Poland and Canada
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Photosensitive and pH-dependent activity of pyrazine-functionalized carbazole derivative as promising antifungal and imaging agent
PublicationCarbazole skeleton plays a significant role as a structural scaffold of many pharmacologically active compounds. Pyrazine-functionalized carbazole derivative was constructed by coupling 2-amino-5-bromo-3-methylaminepyrazine (ABMAP) into 3 and 6 positions of the carbazole ring. Multi-experimental methods were used, e.g., potentiometric, spectroscopic (ATR, UV, XRD powder,1H and13C NMR), electrochemical (cyclic voltammetry), and...
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Rapid communication: Computational simulation and analysis of a candidate for the design of a novel silk-based biopolymer
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Development of potential candidate reference materials for drugs in bottom sediment, cod and herring tissues
PublicationRegular use of a reference material and participation in a proficiency testing program can improve the reliability of analytical data. This paper presents the preparation of candidate reference materials for the drugs metoprolol, propranolol, carbamazepine, naproxen, and acenocoumarol in freshwater bottom sediment and cod and herring tissues. These reference materials are not available commercially. Drugs (between 7 ng/g and 32...
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Cu supported on various oxides as a candidate catalyst for dry methane reforming in DIR-SOFCs systems
PublicationA series of Cu-support systems were tested as potential candidates for DIR-SOFC (Direct Internal Reforming SOFC) catalysts towards a dry reforming of methane (DRM). The various supports (-Al2O3, CeO2, ZrO2, SrTiO3) with comparable specific surface area (SSA), and additionally -Al2O3 with SSA an order of magnitude larger than that of the other supports has been applied. The obtained Cu-support systems were characterized in terms...
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Dyfuzyjne spalanie turbulentne metanu w strudze: analiza numeryczna płomienia Sandia D
PublicationDyfuzyjne spalanie turbulentne jest najpowszechniej występującym w zastosowa-niach przemysłowych rodzajem tego procesu. Jednak jego dokładny opis wciąż sprawia trudności i obserwuje się rozwój konkurujących modeli implementowanych w podejściu RANS (Reynolds Averaged Navier Stokes), a współcześnie coraz częściej także w metodzie dużych wirów (LES) o czym świadczą liczne publikacje [13, 16, 18, 21]. W niniejszej pracy przedstawiono...
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Three Editions of Inter-University Studies on Space and Satellite Technology. Candidate and/vs. Graduate, a Case Study
PublicationCurrently, there is a growing demand for most up-to-date academic courses that will fulfil the needs of modern society. Each candidate has to make choices and judgements carefully, in order to succeed on the market. This is particularly important when educating individuals with different backgrounds, especially on an inter-university course in the field of space sciences and technology. This paper describes a case study carried...
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Homogeneity study of candidate reference material (contaminated soil) based on determination of selected metals, PCBs and PAHs
PublicationReference materials (RMs) are an indispensable part of QA/QC system and have played a critical role in the continual expansion of analytical capabilities of the analytical laboratories. The homogeneity study of the candidate for reference material is essential for the quality of reference material (certified reference material) used in quality assurance activities. The homogeneity study of candidate for reference materials of contaminated...
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Evaluation of candidate reference material obtained from selenium - enriched sprouts for the purpose of selenium speciation analysis
PublicationSelenium is an essential element in animals and humans. Diet supplementation with selenium-rich vegetables has better chemoprotective effect than Se (IV) and SeMet. The production of selenium-enriched vegetables requires a preliminary stage in soil preparation, which may subsequently pose a risk of soil contamination. It seems that the sprouting of seeds under controlled conditions would be a good solution for delivering SeMetSeCys...
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Multilocus Analysis of Candidate Genes Involved in Neurogenic Inflammation in Pediatric Asthma and Related Phenotypes: A Case–Control Study
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Determining the optimal course alteration maneouvre in a multi-target encounter situation for a given ship domain model
PublicationW artykulee przedstawiono nową deterministyczną metodę wyznaczania niezbędnego manewru kursem dla sytuacji spotkania z wieloma obiektami obcymi i dla dowolnej zadanej domeny. Jej prostota i niska złożoność obliczeniowa czynią ją dobrą alternatywą dla obecnie stosowanych metod. Główny algorytm został przedstawiony wprost, tak aby mógł być bezpośrednio zastosowany w pokładowych systemach antykolizyjnych lub w systemach VTS.
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Elemental composition, environment of deposition of the Lower Carboniferous Emma Fiord Formation oil shale in Arctic Canada
PublicationThe sedimentary succession of 51-m consisting of a thin coal seam (1 m) and oil shale with a marlstone and carbonate-mudstone matrix of the Lower Carboniferous (Viséan) Emma Fiord Formation located on the Grinnell Peninsula, Devon Island, Arctic Canada was examined. The techniques used include reflected light microscopy, and instrumental neutron activation analysis (INAA) for elemental concentration, and inductively coupled plasma...
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High Cu content LaNi1-xCuxO3-δ perovskites as candidate air electrode materials for Reversible Solid Oxide Cells
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Ruddlesden-Popper-type Nd2-xNi1-yCuyO4±δ layered oxides as candidate materials for MIEC-type ceramic membranes
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Development of a Peptide Derived from Platelet-Derived Growth Factor (PDGF-BB) into a Potential Drug Candidate for the Treatment of Wounds
PublicationObjective: This study evaluated the use of novel peptides derived from platelet-derived growth factor (PDGF-BB) as potential wound healing stimulants. One of the compounds (named PDGF2) was subjected for further research after cytotoxicity and proliferation assays on human skin cells. Further investigation included evaluation of: migration and chemotaxis of skin cells, immunological and allergic safety, the transcriptional analyses...