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Cloning, expression, purification and characterization of recombinant trehalose synthase from Deinococcus radiodurans.
PublikacjaTrehalose (α-D-glucopyranosyl-1,1-α-D-glucopyranoside) is a nonreducing disacharide in which the two glucose molecules are linked trough a α-1,1-glycosidic bond. Trehalose is readily hydrolyzed to glucose and can be used as a reserve of that sugar in the cell. The presence of trehalose was found in the cells of fungi and yeasts, bacteria, nematodes, insects, eggs, pupae and some plants. The characteristics of trehalose make it...
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Effects of social media communication on brand equity and brand purchase intention
PublikacjaThe author studied the effect of two different social media communication on brand equity and brand purchase intention. A total of 504 data sets were generated through a standardized online-survey and analysed with structural equation modeling. The results of the empirical study showed that both firm-created and user-generated social media communication influenced brand equity, consequently impacting brand purchase intention. This...
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MutL protein as a constituent of vsp, ner and mmr repair systems
PublikacjaMutS and MutL proteins are renowned mostly for their functions in well-characterized, post-DNA replication mis- match repair system (MMR). However, there is growing evidence that MMR system is not the only field of action for these pro- teins. Moreover, the participation in MMR does not even have to be their primary function. There are some reports indicat- ing involvement of MutL in BER, NER and VSP (very short patch repair)....
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Robustness of quantum-randomness expansion protocols in the presence of noise
PublikacjaIn this paper we investigate properties of several randomness generation protocols in the device independent framework. Using Bell-type inequalities it is possible to certify that the numbers generated by an untrusted device are indeed random. We present a selection of certificates which guarantee two bits of randomness for each run of the experiment in the noiseless case and require the parties to share a maximally entangled state....
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Rekonstrukcja światowego rozkładu dochodów na podstawie minimalnej informacji statystycznej
PublikacjaCelem pracy jest uzyskanie próby ze światowego rozkładu dochodów (WID). Zakładamy, że krajowe rozkłady dochodów są log-logistyczne z jednostkową średnią. Stąd wynika, że takie rozkłady są całkowicie określone przez indeks Giniego. Oceny indeksu Giniego dla 119 krajów w latach 1990-2005uzyskaliśmy z baz danych Deningera-Squire’a, WID2 i innych baz. Wygenerowaliśmy próbę losową z każdego rozkładu i przemnożyliśmy jej wartości przez...
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Facial emotion recognition using depth data
PublikacjaIn this paper an original approach is presented for facial expression and emotion recognition based only on depth channel from Microsoft Kinect sensor. The emotional user model contains nine emotions including the neutral one. The proposed recognition algorithm uses local movements detection within the face area in order to recognize actual facial expression. This approach has been validated on Facial Expressions and Emotions Database...
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Export diversification and economic development: a dynamic spatial data analysis
PublikacjaThis paper contributes to the empirical literature on the relationship between ‘export variety’ (export diversification) and economic development by relaxing the assumption of cross-country independence and allowing for spatial diffusion of shocks in observed and unobserved factors. Export variety is measured for a balanced panel of 114 countries (1992-2012) using very detailed information on their exports (HS 6-digit product...
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Particle Shape Effect on Macroscopic Behaviour of Underground Structures: Numerical and Experimental Study
PublikacjaAbstract: The mechanical performance of underground flexible structures such as buried pipes or culverts made of plastics depend not only on the properties of the structure, but also on the material surrounding it. Flexible drains can deflect by 30% with the joints staying tight, or even invert. Large deformations of the structure are difficult to model in the framework of Finite Element Method, but straightforward in Discrete...
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Produktywność dydaktyczna publicznych uniwersytetów w latach 2010–2015
PublikacjaCelem podjętych badań było określenie zmian w produktywności publicznych uniwersytetów w zakresie działalności dydaktycznej. W badaniu wykorzystano nieradialny indeks Malmquista SBM zorientowany na wyniki. W analizach uwzględniono 18 uczelni będących pod nadzorem Ministra Nauki i Szkolnictwa Wyższego. W latach 2010–2015 przeciętna wartość indeksu Malmquista wynosiła 0,90, co wskazuje na pogorszenie produktywności uniwersytetów....
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Badania bioaktywności z wykorzystaniem komórek kościotwórczych na tytanie pokrytym chitozanem = Bioactivity studies using osteogenic cells on titanium coated with chitosan
PublikacjaChitosan is a natural polymer from the group of polysaccharides, which has found wide application in biomedical applications to induce bioactivity of implant. The studies described were carried out test imposition of chitosan membranes with varying degrees of deacetylation on the surface of titanium, and then check the bioactivity of the material in vitro, using osteogenic cells MC3T3-E1. Titanium surface before imposition of...
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Fibrillar aggregates in powdered milk
PublikacjaThis research paper addresses the hypothesis that powdered milk may contain amyloid fibrils. Amyloids are fibrillar aggregates of proteins. Up to this time, research on the presence of amyloids in food products are scarce. To check the hypothesis we performed thioflavin T fluorescence assay, X-ray powder diffraction, atomic force microscopy and fluorescence microscopy imaging. Our preliminary results show that commercially available...
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Does the low optical band gap of yellow Bi3YO6 guarantee the photocatalytical activity under visible light illumination?
PublikacjaBi3YO6, which is known as an ionic conductor, was tested here as an electrode and photoanode in contact with aqueous electrolytes. Bi3YO6 was deposited onto the Pt substrate and the such prepared electrode was polarized in various aqueous electrolytes. The optical energy band gap of the material equal to 1.89 eV was determined using the Kubelka-Munk function resulting from the UV-Vis spectrum (allowed indirect transition) and also...
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Aminy w środowisku - niebezpieczeństwo czy jego naturalny element?
PublikacjaA lot of amines and their compounds are used in the industry. First of all they are used for the preparation of medicines, plant protection agents, fertilizers and plastics. Amines are also released into the environment as a result of natural biological processes such as the release of certain species of flowers to lure insects, and also biochemical breakdown of amino acids in the protein during decomposition of organic matter. Unfortunately,...
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Consumerism and the Quality of Life
PublikacjaHigh level of consumption, driven by marketing activities, the pleasure and joy of possession and the accumulation of material goods are often associated with prosperity, sense of happiness and fulfilment in life. On a broader scale, economic indicators related to production and consumption are used to define the well-being and quality of life in societies. Unfortunately, the phenomenon of consumerism entails negative social and...
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Determination of the minimal critical rotational speeds of the circular saw blades with the quasi-twin resonant frequencies
PublikacjaTo determine the minimal critical rotational speeds of the circular saw blades is the fundamental aspect of obtaining the range of the rotational operating speeds, by which the circular saw blade can work with required stability. While for the circular saw blades with full-homogeneous bodies the determination of such rotational speeds is the relatively low level of difficulty function, whereas for circular saw blades with more...
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Balancing Energy Inputs/Outputs and GHG Emissions for Sustainable Wastewater Treatment – a Case Study
PublikacjaThe aim of the study is a model-based evaluation of the selected operating modes and technological upgrades on the energy balance and greenhouse gas (GHG) emissions in a large wastewater treatment plant in the city of Slupsk (northern Poland). The considered scenarios focus on energy savings for aeration, increasing energy recovery via biogas cogeneration and reduction of the CO2 equivalent emission from both direct processes (related...
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Beneficial effects and potential risks of tomato consumption for human health: An overview
PublikacjaTomato and its derived products have a very interesting nutritional value in addition to prominent antioxidant,anti-inflammatory, and anticancer activities. In terms of tomatoes are generally quite safe to eat. However,overall consumption varies from individual to individual. Indeed, either beneficial or harmful effects of plantsor their derived products are closely related to quality, including the presence of biologically active...
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Molecular Umbrella as A Nanocarrier for Antifungals
PublikacjaA molecular umbrella composed of two O‐sulfated cholic acid residues was applied for the construction of conjugates with cispentacin, containing a “trimethyl lock” (TML) or o‐dithiobenzylcarbamoyl moiety as a cleavable linker. Three out of five conjugates demonstrated antifungal in vitro activity against C. albicans and C. glabrata but not against C. krusei, with MIC90 values in the 0.22–0.99 mM range and were not hemolytic. Antifungal...
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Functionalization of graphene oxide coatings with phosphorus atoms and their corrosion resistance in sodium chloride environment
PublikacjaIn this work, we compared corrosion resistance of graphene oxide, and phosphorus functionalized reduced graphene oxide coatings obtained as a result of electrophoretic deposition on the copper substrate. Doping graphene oxide with phosphorus atoms was performed by a hydrothermal method in the presence of different amounts of phosphoric acid. Structural studies confirmed the insertion of phosphorus atoms into the graphene oxide...
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Methodology for hospital design in architectural education
PublikacjaThe architecture of a hospital should be a response to strong user requirements. Recommendations on how to shape the environment of such facilities are highly complex, integrating guidelines from many fields of science. If contradictions between them exist, the designer is required to set priorities for spatial activities. This issue is particularly important during architectural education. The learning process should include projects...
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Particle Shape Effect on Macroscopic Behaviour of Underground Structures: Numerical and Experimental Study
PublikacjaThe mechanical performance of underground flexible structures such as buried pipes or culverts made of plastics depend not only on the properties of the structure, but also the material surrounding it. Flexible drains can deflect by 30% with the joints staying tight, or even invert. Large deformations of the structure are difficult to model in the framework of Finite Element Method, but straightforward in Discrete Element Methods....
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Determinants of Firm Innovation in Indonesia: The Role of Institutions and Access to Finance
PublikacjaThis paper investigates the determinants of firm innovation in Indonesia. Using quality of local regulations index constructed by the Indonesia’s Regional Autonomy Watch (KPPOD) as measure of institutions, We found that better institutional quality at the local level was associated with more innovation and that firms experiencing major obstacle in access to finance were less likely to innovate. Access to finance is more critical...
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Subsoil degradation effect in reliability analysis of the jack-up platform structure
PublikacjaStructural reliability analysis is considered, by FORM and SORM applied to a certain idealized soil-platform structure interaction problem due to cyclic water wave and wind loads. Wave and wind loads are random variables, whereas dead load is deterministic. Load parameters are typical for storm conditions in the Baltic Sea. The soil-structure interaction is idealized as a set of linear translational and rotational springs. Due...
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Time-frequency analysis of acoustic signals using concentrated spectrogram
PublikacjaThe paper presents improved method of time-frequency (TF) analysis of discrete-time signals. The method involves signal's local group delay (LGD) and channelized instantaneous frequency (CIF) to purposely redistribute all Short-time Fourier transform (STFT) lines. Additionally, the energy concentration index (ECI) and some histogram-like statistics are used to evaluate readability of estimated TF distributions of the energy. Recorded...
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Investigations on mixture preparation for two phase adiabatic pressure drop of R134a flowing in 5 mm diameter channel
PublikacjaThe article presents detailed two-phase adiabatic pressure drops data for refrigerant R134a. Study cases have been set for a mass flux varying from 200 to 400 kg/m2 s, at the saturation temperature of 19.4 ◦C. Obtained experimental data was compared with the available correlations from the literature for the frictional pressure drop during adiabatic flow. Influence of mixture preparation on pressure drop was investigated, for...
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How Does International Competitiveness Affect Economic Development? A Two-Phase Hypothesis
PublikacjaThis paper analyses the impact of international competitiveness on economic development, based on the World Economic Forum Global Competitiveness Index (GCI) and GDP/capita of 125 countries during the period 2007-2010. The results reveal two phases of the evolution of competitiveness. When GCI < 4.5 (on 1-7 scale), any improvement of a country’s competitiveness affects current development more than future development. When...
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Opto-Electrochemical Sensing Device Based on Long-Period Grating Coated with Boron-Doped Diamond Thin Film
PublikacjaThe fabrication process of thin boron-doped nanocrystalline diamond (B-NCD) microelectrodes on fused silica single mode optical fiber cladding has been investigated. The B-NCD films were deposited on the fibers using Microwave Plasma Assisted Chemical Vapor Deposition (MW PA CVD) at glass substrate temperature of 475 ºC. We have obtained homogenous, continuous and polycrystalline surface morphology with high sp3 content in B-NCD...
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Nanocrystalline diamond microelectrode on fused silica optical fibers for electrochemical and optical sensing
PublikacjaFabrication process of thin boron-doped nanocrystalline diamond (B-NCD) microelectrode on fused silica single mode optical fiber has been investigated. The B-NCD films were deposited on the fibers using Microwave Plasma Assisted Chemical Vapor Deposition (MW PA CVD) at glass substrate temperature of 475 ºC. We have obtained homogenous, continuous and polycrystalline surface morphology with the mean grain size in the range of 100-250...
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Fire-Retardant Flexible Foamed Polyurethane (PU)-Based Composites: Armed and Charmed Ground Tire Rubber (GTR) Particles
PublikacjaInadequate fire resistance of polymers raises questions about their advanced applications. Flexible polyurethane (PU) foams have myriad applications but inherently suffer from very high flammability. Because of the dependency of the ultimate properties (mechanical and damping performance) of PU foams on their cellular structure, reinforcement of PU with additives brings about further concerns. Though they are highly flammable and...
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Effect of small quantities of potassium promoter and steam on the catalytic properties of nickel catalysts in dry/combined methane reforming
PublikacjaCarbon dioxide and methane are two of the principal greenhouse gases. Reduction of their content in the atmosphere is currently the subject of much worldwide research. Dry and combined reforming of methane are effective methods of CO2 and CH4 utilization and production of synthesis gas (syngas) in chemical technology. Testing of catalysts that provide the desired H2/CO ratio and long operation time is one of the critical aspects...
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Reliability of Corroded Stiffened Plate Subjected to Uniaxial Compressive Loading
PublikacjaThe work is focused on the reliability of corroded stiffened plates subjected to compressive uniaxial load based on the progressive collapse approach as stipulated by the Common Structural Rules for Bulk Carriers and Oil Tankers, employing the limit state design. Two different cases have been investigated. In the first model, the corrosion degradation led to uniform thickness loss, whereas the mechanical properties were unchanged,...
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CONSUMERS’ APPROACH TO THE CREDIBILITY OF THE INFLATION FORECASTS PUBLISHED BY CENTRAL BANKS: A NEW METHODOLOGICAL SOLUTION
PublikacjaModern monetary policy focuses on credibility and shaping inflation expectations. In keeping with the concept of inflation forecast targeting, the inflation forecasts published by central banks play a crucial role in the instrument rate decision-making process and may be treated as a specific intermediate target. This study proposes an inflation forecast credibility index, the scope of which is narrowed to non-specialists’ approach...
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The Emergence of ETFs in Asia-Pacific
PublikacjaThe major objective of this book is to contribute to the present state of knowledge by exploring empirical links between the processes of diffusion of financial innovations— exchange-traded funds—and growing deployment of ICT across Asia-Pacific countries, between 2004 and 2017. We examine the evolutionary patterns of ETFs on selected Asia-Pacific stock exchanges and verify the hypothesized relationship between increasing ICT penetration...
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Mitigation of the Flow Maldistribution in Minichannel and Minigap Heat Exchangers by Introducing Threshold in the Manifolds
PublikacjaIn the present paper, a detailed numerical investigation has been carried out to analyze the flow maldistribution in 50 parallel rectangular cross-section (1 mm depth and 1 mm width) minichannels and minigap section (1 mm depth and 99 mm width) with rectangular/trapezoidal manifolds in Z-type flow configuration. The author carried out numerical investigation with various mass flowrates, namely 0.05 kg/s, 0.1 kg/s and 0.2 kg/s which...
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Structure and properties comparison of poly(ether-urethane)s based on nonpetrochemical and petrochemical polyols obtained by solvent free two-step method
PublikacjaThe application of thermoplastic polyurethanes (TPU) is becoming more and more extensive, and the decreasing of used petrochemical monomers and reduction of energy for the polymerization and processing processes is getting increasingly important. In this paper, we confirmed the positive influence of high bio-based monomers contents (by replacing petrochemical polyol and glycol by bio-based counterparts) on processing and properties...
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Carcinoid tumor, NOS - Female, 46 - Tissue image [12060630017634441]
Dane BadawczeThis is the histopathological image of BRONCHUS AND LUNG tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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One Europe, one product, two prices - the price disparity in the EU
PublikacjaThe price dispersion in the European Union in the last fifteen years (1990-2005) is analysed. The analysis of price convergence is examined on aggregate and disaggregate levels. The macro approach is based on Comparative Price Level index calculated as the ratio between PPPs and exchange rate. The disaggregate analysis utilizesactual prices of 148 individual products sold in the 15 capital cities of the EU. The calculations comprise...
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The conditions of the business services sector in Poland and its competitiveness in the global market
PublikacjaThe following article presents the conditions of the business services sector (BSS) in Poland and the assessment of its competitiveness in the global market. The first section discusses the BSS sector and the categories of the provided services, in the division of business process outsourcing, shared services center, information technology outsourcing, research and development. Next the authors presents the status of the business...
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Wind Conditions in Urban Layout – Numerical and Experimental Research
PublikacjaThis paper presents research which compares the numerical and the experimental results for different cases of airflow around a few urban layouts. The study is concerned mostly with the analysis of parameters, such as pressure and velocity fields, which are essential in the building industry. Numerical simulations have been performed by the commercial software Fluent, with the use of a few different turbulence models, including...
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COMPUTER ANALYSIS OF HYDRAULIC CONDITIONS IN A SANITARY CHAMBER
PublikacjaThis paper presents analysis of hydraulic conditions of sewage flow down the slipway (ramp) in the collecting sanitary chamber connecting 3 collectors on the inlet: ⌀600, ⌀800 and ⌀1400 and one on the outlet ⌀1600 mm, with a maximum difference of elevation 5 m. The mathematical model has been made and hydraulic calculations were carried out in steady flow conditions. Then wastewater surface elevation, energy grade line elevation,...
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Influence of Thermal Decomposition of Wood and Wood-Based Materials on the State of the Atmospheric Air. Emissions of Toxic Compounds and Greenhouse Gases
PublikacjaThis paper presents the energy characteristics of wood and wood-based materials in the form of commercially available pellets, furniture board (MDF) and OSB. Toxicometric indices were determined for gaseous destructs arising from thermal decomposition and combustion of the materials studied. The paper proves that combustion conditions are crucial in terms of toxic destructive emissions. It has been shown that the combustion of...
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Facing the brainstorming theory. A case of requirements elicitation
PublikacjaKnowledge is still considered to be power and its externalization makes it possible for others to use that power. In this paper, we examine the theory of brainstorming, and the claim by father Alex Osborn that in a group session an individual can think of twice as many ideas than working alone. In the context of requirements elicitation, we performed an experiment on a “nominal” and a “real” group of participants, following a procedure...
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A Centralized Reputation System for MANETs Based on Observed Path Performance
PublikacjaA reputation system for MANETs is described that attempts to deduce nodal trustworthiness (forwarding behaviour) from observed end-to-end path performance. The trustworthiness deduction algorithm produces interval estimates and works well if node misbehaviour is not selec-tive with respect to traversing paths. Nodal reputation levels are next calculated in the spirit of generous tit-for-tat so as to best reflect momentary nodal...
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Does offshoring affect industry employment? Evidence from a wide European panel countries
Publikacjais paper contributes to the literature on the possible impact of international outsourcing on domestic labour markets. We focus on off shoring-employment relationship. Th e analysis is performed for a wide European panel, composed of 27 EU countries and 13 manufacturing sectors, observed in the period 1995-2009. Th anks to the use of input-output tables from the WIOD project, we measure the intensity of off shoring in the sectors,...
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Detection of Face Position and Orientation Using Depth Data
PublikacjaIn this paper an original approach is presented for real-time detection of user's face position and orientation based only on depth channel from a Microsoft Kinect sensor which can be used in facial analysis on scenes with poor lighting conditions where traditional algorithms based on optical channel may have failed. Thus the proposed approach can support, or even replace, algorithms based on optical channel or based on skeleton...
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Local properties of organic coatings close to glass transition temperature
PublikacjaAcrylic coating applied on copper substrate has been investigated at different temperatures starting from the ambient one up to the level above the glass transition temperature. Its local topographic and electric properties have been examined with the atomic force microscopy (AFM) based approach including dc current mapping and local impedance spectroscopy. The local findings have been compared with the information from global...
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PHONEME DISTORTION IN PUBLIC ADDRESS SYSTEMS
PublikacjaThe quality of voice messages in speech reinforcement and public address systems is often poor. The sound engineering projects of such systems take care of sound intensity and possible reverberation phenomena in public space without, however, considering the influence of acoustic interference related to the number and distribution of loudspeakers. This paper presents the results of measurements and numerical simulations of the...
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Introduction of Pro and Its Analogues in the Conserved P1 Position of Trypsin Inhibitor SFTI-1 Retains Its Inhibitory Activity
PublikacjaA number of monocyclic SFTI-1 analogues modified in the conserved inhibitor P1 position by Pro, its L-hydroxyproline (Hyp) derivative as well as mimetics with different ring size were synthesized by the solid-phase method. Replacement of Ser6 by Pro, Hyp, and a four-member ring, L-azetidine-2-carboxylic acid (Aze), retained trypsin or chymotrypsin inhibitory activity. The determined association equilibrium constants of these analogues...
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COMPARATIVE ANALYSES OF INTERNATIONALIZATION OF KAZAKHSTAN ENTERPRISES
PublikacjaThis paper presents the instruments of internationalization of enterprises operating within the Eurasian Economic Union. Research contains answers of 108 enterprises exporters located in the Republic of Kazakhstan. The purpose of the paper is to show the main forms of the internationalization of enterprises in Kazakhstan. The paper also presents the following instruments of enterprises’ internationalization including: indirect...
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Protein overproduction alters exosome secretion in Chinese hamster ovary cells
PublikacjaDespite the abundance of available cell lines, nearly 70% of all recombinant therapeutic proteins today are produced in Chi-nese hamster ovary (CHO) cells. The impact of protein overproduction on the secretion of exosomes by CHO cells has been investigated here. Increased secretion of extracellular vesicles (EVs) by protein overexpressing CHO cells was demonstrated with protein content assay, nanoparticle tracking analysis, and...