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Identification and molecular modeling of a novel lipase from an Antarctic soil metagenomic library.
PublikacjaIn this work, we present the construction of a metagenomic library in Escherichia coli using pUC19 vector and environmental DNA directly isolated from Antarctic topsoil and screened for lipolytic enzymes. The screening on agar supplemented with olive oil and rhodamine B revealed one clone with lipolytic activity (Lip1) out of 11,000 E. coli clones. This clone harbored a plasmid, pLip1, which has an insert of 4722 bp that has been...
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Analysis of Interspike-Intervals for the General Class of Integrate-and-Fire Models with Periodic Drive
PublikacjaWe study one-dimensional integrate-and-fire models of the general type x˙=F (t, x) and analyze properties of the firing map which iterations recover consecutive spike timings. We impose very week constraints for the regularity of the function F (t, x), e.g. often it suffices to assume that F is continuous. If additionally F is periodic in t, using mathematical study of the displacement sequence of an orientation preserving circle...
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Conservation and diversity of MutS proteins
PublikacjaThe homologues of MutS, mismatch repair protein, exist in all prokaryotes, with the exception of Actinobacteria, Mollicutes and part of the Archaea. Multiple alignments of 316 MutS amino acid sequences from 169 species revealed conserved residues and sequence motifs distinguishing MutS homologues. All MutS homologues show high conservation within the ATPase domain. MutS1, the homologue responsible for DNA mismatch recognition,...
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DETERMINING THE TIME CONSTANT USING TWO METHODS AND DEFINING THE THERMOCOUPLE RESPONSE TO SINE EXCITATION OF GAS TEMPERATURE
PublikacjaThis paper describes the two methods of determining the time constant of type K thermocouple, for two construction solutions: the exposed weld and the mantle fillet weld. This is an important parameter indicating the response time of the thermocouple on the recorded signal. The first method consists of determining the value of τ in the way of numerical simulation of heat exchange between the thermocouple and the current of gas...
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Assessment of diversity and composition of bacterial community in Sludge Treatment Reed Bed systems
PublikacjaDue to their low emission of odours and lack of the need to apply additional chemical agents, sludge treatment reed beds (STRBs) constitute an economically feasible and eco-friendly approach to sewage sludge management. Correctly designed and operated STRBs ensure effective reduction of the dry matter content coupled with the mineralisation of organic compounds. Successful operation of STRBs relies on complex interactions between...
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Asymptotic Expansion Method with Respect to Small Parameter for Ternary Diffusion Models
PublikacjaTernary diffusion models lead to strongly coupled systems of PDEs. We choose the smallest diffusion coefficient as a small parameter in a power series expansion whose components fulfill relatively simple equations. Although this series is divergent, one can use its finite sums to derive feasible numerical approximations, e.g. finite difference methods (FDMs).
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Experiments on Preserving Pieces of Information in a Given Order in Holographic Reduced Representations and the Continuous Geometric Algebra Model
PublikacjaGeometric Analogues of Holographic Reduced Representations (GAc, which is the continuous version of the previously developed discrete GA model) employ role-filler binding based on geometric products.Atomic objects are real-valued vectors in n-dimensional Euclidean space and complex statements belong to a hierarchy of multivectors. The property of GAc and HRR studied here is the ability to store pieces of information in a given...
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Structural features responsible for control of the eukaryotic GlcN-6-P synthase activity
PublikacjaGlucosamine-6-phosphate (GlcN-6-P) synthase is responsible for catalysis of the first and practically irreversible step in hexosamine metabolism. As UDP-GlcNAc, the final product of the hexosamine path, constitutes one of the essential substrates for assembly of bacterial and fungal cell walls, the enzyme is an interesting target for antimicrobial therapy.The structure of E. coli enzyme, known since 2001, forms a dimer of two identical...
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Determination of long-chain aldehydes using a novel quartz crystal microbalance sensor based on a biomimetic peptide
PublikacjaThere is an increasingly popular trend aimed at improvement of fundamental metrological parameters of sensors via implementation of materials mimicking biological olfactory systems. This study presents investigation on usefulness of the peptide mimicking HarmOBP7 region as a receptor element of the piezoelectric sensor for selective analysis of long-chain aldehydes. Identification of odorant binding proteins creates new possibilities...
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Structure and the space vector modulation for a medium-voltage power-electronic-transformer based on two seven-level cascade H-bridge inverters
PublikacjaThis study presents the structure and the space vector pulse-width modulation (SVPWM) for power electronic transformer (PET) based on two seven-level cascade H-bridge (CHB) inverters. The DC links of CHB inverters are coupled with nine dual-active bridge (DAB) converters with medium-frequency transformers. The DC-link voltages are equalised with two methods – through the control of DAB voltages...
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MutS3: a MutS homologue of unknown biological function
PublikacjaThe homologues of MutS proteins are widespread among both Prokaryotes and Eukaryotes. MutS designated as MutS1 is a part of MMR (mismatch repair) system which is responsible for removal of mispaired bases and small insertion/deletion loops in DNA. Initially, the only MutS homologues known were those engaged in mismatch repair and these were later designated as MutS1. Subsequently, the MutS2 homologue was distinguished. MutS2 does...
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Lekka obudowa w nadbudowach i renowacjach
PublikacjaNadbudowy i renowacje – wykorzystanie technologii lekkiej obudowy. W artykule przedstawiono możliwości wykorzystania technologii lekkiej obudowy do nadbudowy i renowacji obiektów budowlanych. Opisano kolejne kroki przygotowania inwestycji dotyczącej projektowania i realizacji nadbudowy obiektu budowlanego.
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Biowodór, „paliwo przyszłości”, dotychczasowe metody otrzymywania , porównanie metod otrzymywania
PublikacjaWodór jest ważnym pierwiastkiem w przemyśle chemicznym. Biowodór wydaje się być użytecznym odnawialnym nośnikiem energii, który mógłby zastąpić paliwa kopalne. Największym problemem wodoru jest jego rzadkie występowanie w czystej formie molekularnej H 2 . Dlatego otrzymanie biowodoru w objętościach opłacalnych ja ko surowiec czy biopaliwo wiedzie przez bioodpady „przesycone” wodorem. Każdy związek...
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Wykorzystanie słabych sygnałów, foresightu i ciągu zarządzania strategicznego w konfiguracji biznesu przyszłości
PublikacjaBurzliwość współczesnego otoczenia wyostrza zagadnienie przystosowania prowadzonej działalności gospodarczej do funkcjonowania w przyszłości. Do osiągnięcia sukcesu w biznesie przyszłości przyczynić się może wykorzystanie dostępnej już obecnie wiedzy. Na szczególną uwagę zasługuje teoria słabych sygnałów, metoda foresight oraz ciąg zarządzania strategicznego, które w połączeniu umożliwiają wypracowanie stanowiska w sprawie kształtu...
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On the interspike-intervals of periodically-driven integrate-and-fire models
PublikacjaWe analyze properties of the firing map, which iterations give information about consecutive spikes, for periodically driven linear integrate-and-fire models. By considering locally integrable (thus in general not continuous) input functions, we generalize some results of other authors. In particular, we prove theorems concerning continuous dependence of the firing map on the input in suitable function spaces. Using mathematical...
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Small Antenna Design Using Surrogate-Based Optimization
PublikacjaIn this work, design of small antennas using efficient numerical optimization is investigated. We exploit variable-fidelity electromagnetic (EM) simulations and the adaptively adjusted design specifications (AADS) technique. Combination of these methods allows us to simultaneously adjust multiple geometry parameters of the antenna structure of interest in a computationally feasible manner, leading to substantial reduction of the...
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Typing of Candida isolates from patients with invasive infection and concomitant colonization
PublikacjaWe investigated the relationship between colonizing and invasive isolates from patients with candidaemia. Molecular typing was performed using random amplification of polymorphic DNA (RAPD) and multilocus sequence typing (MLST). We found MLST to be sufficient for typing Candida isolates, and that surveillance cultures are helpful in predicting concomitant invasive isolates, but not necessarily the pathogen involved in subsequent...
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Amplifying the Randomness of Weak Sources Correlated With Devices
PublikacjaThe problem of device-independent randomness amplification against no-signaling adversaries has so far been studied under the assumption that the weak source of randomness is uncorrelated with the (quantum) devices used in the amplification procedure. In this paper, we relax this assumption, and reconsider the original protocol of Colbeck and Renner using a Santha-Vazirani (SV) source. To do so, we introduce an SV-like condition...
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Characterization of exceptionally thermostable single-stranded DNA-binding proteins from Thermotoga maritima and Thermotoga neapolitana
PublikacjaBACKGROUND: In recent years, there has been an increasing interest in SSBs because they find numerous applications in diverse molecular biology and analytical methods.RESULTS: We report the characterization of single-stranded DNA binding proteins (SSBs) from the thermophilic bacteria Thermotoga maritima (TmaSSB) and Thermotoga neapolitana (TneSSB). They are the smallest known bacterial SSB proteins, consisting of 141 and 142 amino...
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No-local-broadcasting theorem for multipartite quantum correlations
PublikacjaWe prove that the correlations present in a multipartite quantum state have an operational quantum character even if the state is unentangled, as long as it does not simply encode a multipartite classical probability distribution. Said quantumness is revealed by the new task of local broadcasting, i.e., of locally sharing preestablished correlations, which is feasible if and only if correlations are stricly classical. Our operational...
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Multichannel Human Body Communication
PublikacjaHuman Body Communication is an attractive alternative for traditional wireless communication (Bluetooth, ZigBee) in case of Body Sensor Networks. Low power, high data rates and data security makes it ideal solution for medical applications. In this paper, signal attenuation for different frequencies, using FR4 electrodes, has been investigated. Performance of single and multichannel transmission with frequency modulation of analog...
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Systematic approach to binary classification of images in video streams using shifting time windows
Publikacjain the paper, after pointing out of realistic recordings and classifications of their frames, we propose a new shifting time window approach for improving binary classifications. We consider image classification in tewo steps. in the first one the well known binary classification algorithms are used for each image separately. In the second step the results of the previous step mare analysed in relatively short sequences of consecutive...
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On the existence of homoclinic type solutions of inhomogenous Lagrangian systems
PublikacjaWe study the existence of homoclinic type solutions for a class of inhomogenous Lagrangian systems with a potential satisfying the Ambrosetti-Rabinowitz superquadratic growth condition and a square integrable forcing term. A homoclinic type solution is obtained as a limit of periodic solutions of an approximative sequence of second order differential equations.
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Monitoring of single-side lap grinding with electroplated tools
PublikacjaThe results presented in the paper show that utilising microphones as audible sound sensors is a suitable approach for monitoring a single-side lap grinding process due to the low levels of noise generated by the drives of a machine tool. The results confirm that sound signal analysis is a feasible and relatively simple method to monitor a lap grinding process with the use of an audible sound sensor. Proposed method can be used...
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In vitro studies of antimicrobial activity of Gly-His-Lys conjugates as potential and promising candidates for therapeutics in skin and tissue infections
PublikacjaWe presented in vitro studies of antimicrobial activity of Gly-His-Lys conjugates that are important point in preliminary biological evaluation of their potential application in skin and tissue therapy. The novel compounds include the conjugation of fatty acids with a modification of the amino acid sequence in the primary structure of Gly-His-Lys.
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Evidence of mutations conferring resistance to clarithromycin in wastewater and activated sludge
PublikacjaThe occurrence of clarithromycin in wastewater samples and of the activated sludge bacteria possibly resistant to this pharmaceutical was the object of the study. Samples of wastewater or activated sludge were taken from a municipal wastewater treatment plant in summer and winter and characterised regarding their clarithromycin concentrations and the presence of nucleic acid fragments (Cla-sequences) known to be responsible for...
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TRNA-Leu (UUR) gene haplotypes observed in canine mammary gland tumours and its deleterious effect assessment according to the comparative analysis with TRNL1 human gene
Dane BadawczeThe aetiology and pathogenesis of many canine tumours are likely to be similar to cancers found in humans. This study aimed to present a plausible link between changes in tRNA-Leu (UUR) gene and the carcinogenesis process in dogs with mammary gland tumours. The whole mitochondrial DNA (mtDNA) isolated from blood and tumour tissues of 13 dogs with malig-nant...
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Integrated monitoring, control and security of Critical Infrastructure Systems
PublikacjaModern societies have reached a point where everyday life relies heavily on desired operation of critical infrastructures, in spite of accidental failures and/or deliberate attacks. The issue of desired performance operation of CIS at high security level receives considerable attention worldwide. The pioneering generic methodologies and methods are presented in the paper project for designing systems capable of achieving these...
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Algorithms and Tools for Intelligent Control of Critical Infrastructure Systems
PublikacjaCritical Infrastructure Systems (CIS) are spatially distributed and of a network structure. The dynamics are nonlinear, uncertain and with several time scales. There is a variety of different objectives to be reliably met under a wide range of operational conditions. The operational conditions are influenced by the disturbance inputs, operating ranges of the CIS, faults in the sensors and actuators and abnormalities occurring in...
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Structural changes of a simple peptide—Trpzip-1—in aqueous solutions and the corresponding hydration phenomena under the influence of temperature
PublikacjaTrpzip-1, a simple β-hairpin, is a rare example of peptide with stable secondary structure and can be a convenient model to study temperature-related processes that potential prion or amyloid proteins undergo. Although its sequence is simple, the exact processes which the peptide undergoes in aqueous solutions are quite complex and not well understood. The selection of well-established experimental (DSC, FTIR) and theoretical methods...
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Sequences of internal transcribed spacer 1, 5.8S ribosomal RNA gene and internal transcribed spacer 2 of psychrotolerant pigment-producing yeast-like fungi
Dane BadawczeDNA encoding ITS1-5.8S-ITS2 fragments of psychrotolerant pigment-producing yeast-like fungi named Red, Pink and Black were PCR amplified using ITS1 5' TCCGTAGGTGAACCTGCGG 3' and ITS4 5’ TCCTCCGCTTATTGATATGC 3’ primers and sequenced by Sanger method using the same primers.
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Exploring ontological approach for user requirements elicitation in design of online virtual agents
PublikacjaEffective user requirements elicitation is a key factor for the success of software development projects. There are many qualitative and quantitative research studies that promulgate particular methods and show the application of user requirements elicitation in particular domains. However, few try to eliminate the burden of ambiguity in gathered data, naturally occurring in different groups of stakeholders. This paper deals with...
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Quantum superadditivity in linear optics networks: Sending bits via multiple-access Gaussian channels
PublikacjaSuperadditivity effects of communication capacities are known in the case of discrete variable quantum channels. We describe the continuous variable analog of one of these effects in the framework of Gaussian multiple access channels (MACs). Classically, superadditivity-type effects are strongly restricted: For example, adding resources to one sender is never advantageous to other senders in sending their respective information...
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High Temperature Corrosion Evaluation of Porous Hastelloy X Alloy in Air and Humidified Hydrogen Atmospheres
PublikacjaIn this work a commercially available porous Hastelloy X alloy is characterized in terms of high temperature corrosion resistance. The alloy was oxidized in the temperature range from 500C to 900C in air and humidified hydrogen for 100 hours. Corrosion rates and porosity changes were measured. Microstructural characterization was performed using X-ray diffractometry and scanning electron microscopy. Results show that porous alloys...
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NASBA – IZOTERMICZNA AMPLIFIKACJA RNA
PublikacjaNASBA (ang. Nucleic Acid Sequence-Based Amplification) jest skuteczną i czułą techniką izotermicznej amplifikacji opartej na transkrypcji, służąca głównie do detekcji cząsteczek RNA. Znalazła ona zastosowanie do wykrywania RNA wirusowego, bakteryjnego i grzybiczego w próbkach klinicznych oraz żywości.
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Remote Sensing in Vessel Detection and Navigation
PublikacjaThe Special Issue (SI) “Remote Sensing in Vessel Detection and Navigation” highlighted a variety of topics related to remote sensing with navigational sensors. The sequence of articles included in this Special Issue is in line with the latest scientific trends. The latest developments in science, including artificial intelligence, were used. The 15 papers (from 23 submitted) were published.
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How to deal with knowledge in small companies? Defining emergent KM approach
PublikacjaThis paper examines the concept of emergent KM approach in small companies. The origins of consideration are grounded in the theory of strategic management literature and in particular in the distinction between deliberate versus emergent approach towards strategic planning. Using the methodology of case study, we carried out an explorative research to analyse the characteristics of KM approach in two small companies located in...
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Molecular Recognition in Complexes of TRF Proteins with Telomeric DNA
PublikacjaTelomeres are specialized nucleoprotein assemblies that protect the ends of linear chromosomes. In humans and many other species, telomeres consist of tandem TTAGGG repeats bound by a protein complex known as shelterin that remodels telomeric DNA into a protective loop structure and regulates telomere homeostasis. Shelterin recognizes telomeric repeats through its two major components known as Telomere Repeat-Binding Factors, TRF1...
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Influence of Asphalt Binder on the Compactability of Asphalt Mixtures Using Locking Point
PublikacjaCompaction is an important process for the asphalt mixture service life. A properly compacted mixture is crucial for long-lasting pavement. The objective of this study was to evaluate the influence of asphalt binders on the compactability of the asphalt mixtures by utilizing the concept of the locking point. To achieve this objective, two compaction methods (impact and gyration), different temperatures and asphalt content, and...
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Network Approach to Increments of RR-intervals for Visualization of Dynamics of Cardiac Regulation
PublikacjaThe transition network for RR -increments is pre- sented in a directed and weighted graph, with vertices represent- ing RR -increments and edges corresponding to the order in a sequence of increments. The adjacency matrix and the transition matrix of this network provide a graphical tool which could be useful in the assessment of cardiac regulation. As an example, the method is applied in detecting differences between diurnal activity...
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Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria
PublikacjaSingle-stranded DNA-binding proteins (SSBs) are indispensable elements in the cells of all living organisms. SSB proteins interact with ssDNA insequence in an independent manner, preventing them from forming secondary structures and from degradation by nucleases. In this way, SSB-binding proteins participate in all processes involving ssDNA, such as replication, repair and recombination. Although there are differences in amino...
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Multiprocessor Implementation of Parallel Multiobjective Genetic Algorithm for Optimized Allocation of Chlorination Stations in Drinking Water Distribution System a New Water Quality Model Approach
PublikacjaThe Critical Infrastructure Systems (CISs) have received in recent years a considerable attention due to their heavy impact on sustainable development of modern societies. Most CISs may be classied as large scale complex systems of network structure, in uenced by strong interactions form the surrounding environment, internal and external interconnections. The later is a result of inter-CIS dependencies. The control, monitoring...
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EDISOn Electromagnetic Design of fexIble SensOrs
ProjektyProjekt realizowany w Katedra Inżynierii Mikrofalowej i Antenowej zgodnie z porozumieniem POIR.04.04.00-00-1DC3/16 (nowy nr umowy); TEAM TECH/2016-1/6 (stary nr umowy) z dnia 2016-12-06
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Ontologically Aided Rule Model for the Implementation of ITIL Processes
PublikacjaThe implementation of ITIL processes in IT organizations can be seen as a set of interdependent projects. The sequence of the ITIL implementation relies on such parameters as scope, depth, the capability of processes and the maturity of the organization. These factors can form common patterns of ITIL implementation that might be expressed as rules. Also-to semantically reinforce such a model in its predictiveness and replicativeness-the...
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Radar and Sonar Imaging and Processing
PublikacjaThe 21 papers (from 61 submitted) published in the Special Issue “Radar and Sonar Imaging Processing” highlighted a variety of topics related to remote sensing with radar and sonar sensors. The sequence of articles included in the SI dealt with a broad profile of aspects of the use of radar and sonar images in line with the latest scientific trends. The latest developments in science, including artificial intelligence, were used.
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Loop-mediated isothermal amplification (LAMP) as a diagnostic tool in detection of infectious diseases
PublikacjaLoop-mediated isothermal amplification (LAMP) is a gene amplification method which amplifies DNA with high specificity and efficiency under isothermal conditions. Because of its rapidity and simplicity, it is a valuable diagnostic tool in the early detection and identification of infectious diseases. LAMP method is based on the use of a set of four to six specially designed primers spanning six to eight distinct sequences on the...
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Speace frienly for the blind = Przestrzeń przyjazna dla niewidomych
PublikacjaThe article presents issues connected with accessibility of public space for people with eyesight disabilities. The use of the extravisual spatial stimuli in shaping the urban environment has been analysed. Spaces in which musltisensory spatial reception is feasible become user-friendly, as they come to meet the changing needs of their users. The article introduces a system of textures aiding spatial orientation, navigation and...
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Narrowband Transmission Quality in Presence of Modified IEEE 802.15.4a UWB Signal
PublikacjaThis paper presents results of measurement of narrowband transmission quality in presence of impulse radio UWB interference. In the first part of paper, unmodified IEEE 802.15.4a IR UWB signal is used. Then some modification of transmitted pulse sequence is proposed, which allow to reduce UWB power spectral density at frequency of narrowbandtransmission. Finally narrowband transmission quality in presence of modified ultrawideband...
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Oxidation step in the preparation of benzocamalexin: The crystallographic evidence
PublikacjaThe study of oxidation step in the preparation of benzocamalexin by the α-amidoalkylation–oxidation sequence revealed the formation of perchloro-1,2-phenylene dibenzoate as the product of transformation of tetrachloro- 1,2-benzoquinone applied as the oxidant. The structures of benzocamalexin and perchloro-1,2-phenylene dibenzoate were confirmed by X-ray diffraction analysis. The extraction step in the final isolation of benzocamalexin...
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THE GENE CLONING, OVEREXPRESSION, PURIFICATION AND BIOCHEMICAL CHARACTERISATION OF A NEW COLD-ADAPTED β–GALACTOSIDASE FROM ARTHROBACTER SP. VII-4
PublikacjaINTRODUCTION. β-Galactosidase [EC 3.2.1.23] is an enzyme that catalyzes the hydrolysis of O-glycosidic linkages in galactosides. It is commercially used in dairy industry for the production of milk with reduced lactose content. Potentially, the best method for lactose removal under cooling conditions should be carried out with a cold-adapted enzyme. AIM. The aim of this study was to determine the taxonomic affiliation of the isolate...