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Search results for: THREE-DIMENSIONAL FLUORESCENCE
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An MTA-phosphorylase gene discovered in the metagenomic library derived from Antarctic top soil during screening for lipolytic active clones confers strong pink fluorescence in the presence of rhodamine B.
PublicationIn this work, we present the construction of a metagenomic library in Escherichiacoli using the pUC19 vector and environmental DNA directly isolated fromAntarctic topsoil and screened for lipolytic enzymes. Unexpectedly, the screeningon agar supplemented with olive oil and rhodamine B revealed one unusual pinkfluorescent clone (PINKuv) out of 85 000 clones. This clone harbored a plasmid,pPINKuv, which has an insert of 8317 bp that...
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Determination of Urinary Pterins by Capillary Electrophoresis Coupled with LED-Induced Fluorescence Detector
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Fluorescence use in minimally invasive metabolic and bariatric surgery — a systematic review of the literature
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Fluorescence depolarization in the presence of excitation energy migration in partly ordered polymer films.
PublicationW pracy przedstawiono wyniki pomiarów i symulacji Monte Carlo zaników anizotropii emisji fluorescencji dla układu 3,3'-diethylthiacyanine iodine (barwnik) w osiowo rozciąganych foliach polimerowych.
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Near-Infrared Fluorescence Image-Guided Surgery in Esophageal and Gastric Cancer Operations
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Derivation of the scaling laws used in geotechnical centrifuge modelling-application of dimensional analysis and Buckingham Π theorem
PublicationGeotechnical centrifuge modelling has been a world-wide used technology in physical tests. In this paper a derivation of scaling laws by dimensional analysis for the centrifugal modelling is presented. Basic principles of centrifuge modelling are described. Scaling laws for slow events like consolidation and fast events like dynamic loads are shown. The differences in scale factors for both processes are noticed. The aim of this...
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Three-objective antenna optimization by means of kriging surrogates and domain segmentation
PublicationIn this paper, an optimization framework for multi-objective design of antenna structures is discussed which exploits data-driven surrogates, a multi-objective evolutionary algorithm, response correction techniques for design refinement, as well as generalized domain segmentation. The last mechanism is introduced to constrain the design space region subjected to sampling, which permits reduction of the number of training data samples...
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Quantitative hyperbolicity estimates in one-dimensional dynamics
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Quantum correlations in one-dimensional Wigner molecules
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Analysis and synthesis of structural system dimensional deviations
PublicationBłedne zasady określania dopuszczalnych odchyłek wymiarowych stalowych elementów konstrukcyjnych lub wręcz ich brak często powodują trudności w czasie montażu konstrukcji. W każdym układzie konstrukcyjnym można wyróżnić tzw. Koordynacyjne Wymiary Montażowe (KWM). Odchyłki wymiarowe (tolerancje) KWM decydują o zachowaniu poprawnej geometrii ustroju budowlanego po jego zamontowaniu na placu budowy. KWM tworzą zamknięte ciągi wymiarowe,...
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Two dimensional inverse solution for diagonal pumps
PublicationW pracy przedstawiono rozwiązanie zadania odwrotnego dla wirnika i kierownicy pompy diagonalnej. Dla założonego kształtu powierzchni prądu wyznaczono kształt łopatek obu palisad. Geometria kanałów łopatkowych została wyznaczona w wyniku rozwiązania układu równań zapisanych w krzywoliniowym układzie współrzędnych. W pracy przedstawiono fotografie wirnika wykonanego techniką Rapid Prototyping.
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Treatment of landfill leachate by constructed wetlands: three case studies
PublicationThe performance of three CW systems treating landfill leachate, two located in northern Poland (Szadółki near Gdańsk and Gatka near Miastko) and one in southern Sweden (Örebro), is discussed. The CWs differ in size, hydraulic regime, type of hydrophytic plants and type of leachate pre-treatment before discharging to the CW. The CW in Szadółki consists of two parallel beds with sub-surface, horizontal flow of leachate (HSSF) planted...
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Overview of the three multicriteria approaches applied to a global assessment of analytical methods
PublicationCritical and global evaluation of analytical methods should be one of the primary goals in analytical chemistry. A holistic approach, however, requires a look at the varied features: commonly discussed validation criteria, often underrated practical and economic aspects, and typically overlooked compliance with the principles of green analytical chemistry. Carrying out such an assessment in a critical and transparent way is extremely...
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Design and Experiments of a Piezoelectric Motor Using Three Rotating Mode Actuators
PublicationThis paper represents a numerical and experimental investigation of the multicell piezoelectric motor. The proposed design consists of three individual cells that are integrated into the stator, double rotor, and a preload system combined into a symmetrical structure of the motor. Each of the cells is characterized by a traveling wave and rotating mode motor. A finite element numerical analysis is carried out to obtain optimal...
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Comprehensive two-dimensional gas chromatography for determination of the terpenes profile of blue honeysuckle berries
PublicationTerpenes are the main group of secondary metabolites, which play essential role in human. The establishment of the terpenes profile of berries of different blue honeysuckle cultivars was achieved by headspace solid-phase microextraction coupled with comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (HS-SPME/GCGC-TOFMS). The berries were found to contain 44 terpenes identified by GCGC-TOFMS....
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Second-order Stark effect and polarizability of a relativistic two-dimensional hydrogenlike atom in the ground state
PublicationThe second-order Stark effect for a planar Dirac one-electron atom in the ground state is analyzed within the framework of the Rayleigh-Schrödinger perturbation theory, with the use of the Sturmian series expansion of the generalized Dirac-Coulomb Green's function. A closed-form analytical expression for the static dipole polarizability of that system is found. The formula involves the generalized hypergeometric function ${}_{3}F_{2}$...
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Ethylene decomposition on TiO2 - UV and Fluorescence lamp
Open Research DataData contain chromatograms recorded during decomposition of ethylene flowing over TiO2 through the quartz tubular shape reactor. TiO2 was coated onto glass plates with dimension of 2cmx2cm, in total 3 plates were used and placed inside reactor. Reactor was irradiated by UV (Special 'TL'E, Philips) or fluorescent (Lumilux, OSRAM) 3 ring-shaped lamps,...
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Pounding between Inelastic Three-Storey Buildings under Seismic Excitations
PublicationStructural interactions between adjacent, insufficiently separated buildings have been repeatedly observed during damaging ground motions. This phenomenon, known as the structural pounding, may result in substantial damage or even total collapse of structures. The aim of the present paper is to show the results of the nonlinear numerical analysis focused on pounding between inelastic three-storey buildings under seismic excitations....
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Synthesis of three layer NaxCoO2 (x=0.3, 0.5, 0.6, 0.75, 1.0) and superconductivity in three layer Na0.3CoO2 * 1.3H2O
PublicationDyskutowana jest synteza trójwarstwowego związku sodowo-kobaltowego tlenku NaxCoO2. Związek o zawartości sodu x=0.3 może zostać uwodniony i staje się nadprzewodnikiem z Tc=4.3K. Powierzchnia 3 warstwowego związku charakteryzuje się trójkątną morfologią, podczas gdy dwuwarstwowy związek Na0.7CoO2 - heksagonalną morfologią.
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Michał Lech dr inż.
PeopleMichał Lech was born in Gdynia in 1983. In 2007 he graduated from the faculty of Electronics, Telecommunications and Informatics of Gdansk University of Technology. In June 2013, he received his Ph.D. degree. The subject of the dissertation was: “A Method and Algorithms for Controlling the Sound Mixing Processes by Hand Gestures Recognized Using Computer Vision”. The main focus of the thesis was the bias of audio perception caused...
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Determinants of the surface quality, density and dimensional correctness in selective laser melting of the Ti-13Zr-13Nb alloy
PublicationSelective laser melting is widely used for custom-designed elements. Successful manufacturing depends on laser treatment parameters and material features. This research aimed to determine the effects of laser power, scan time and hatch distance on surface quality, relative density and dimensional precision for cuboids made of the Ti-13Zr-13Nb alloy. The influence of energy density, energy flux and pre-heating was seen to be decisive...
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Treatment of the three strip coplanar lines on the ferrite
PublicationW pracy przedstawiono badania koplanarnej linii paskowej złożonej z trzech pasków umieszczonych na podłużnie magnesowanym podłożu ferrytowym. W analizie badanej linii zastosowano metodę spektralną oraz metodę rodzajów sprzężonych. Współczynniki sprzężenia żyromagnetycznego oraz charakterystyki dyspersyjne zostały określone przy użyciu obu metod.
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Packing three-vertex paths in a subcubic graph
PublicationW pracy rozważany jest problem pakowania scieżek P3 w grafach podkubicznych, pokazano oszacowania dolne na ilość ścieżek w zależności od stopnia spójności grafu oraz minimalnego stopnia.
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Three Principles for Healthy Living with Light and Lighting
PublicationThe lockdown measures applied to cities and towns during the worldwide Covid-19 pandemic have had a widespread impact on people’s lives. Some have found themselves confined to their homes, with limited social contact and a reduced quality of life. Others have found that the lockdown improved their wellbeing, as more time was spent outside, instead of commuting and working in an office building, plus the benefits of spending increased...
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Three levels of fail-safe mode in MPI I/O NVRAM distributed cache
PublicationThe paper presents architecture and design of three versions for fail-safe data storage in a distributed cache using NVRAM in cluster nodes. In the first one, cache consistency is assured through additional buffering write requests. The second one is based on additional write log managers running on different nodes. The third one benefits from synchronization with a Parallel File System (PFS) for saving data into a new file which...
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Standing Waves in One-Dimensional Resonator Contaning an Ideal Isothermal Gas Affected by the Constant Mass Force
PublicationThe study is devoted to standing acoustic waves in one-dimensional planar resonator which containing an ideal gas. A gas is affected by the constant mass force. Two types of physically justified boundary conditions are considered: zero velocity or zero excess pressure at both boundaries. The variety of nodal and antinodal points is determined. The conclusion is that the nodes of pressure and antinodes of velocity do not longer...
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Parametric analysis of Istanbul's Ring Road viaduct for three levels of seismic load
PublicationThe paper presents a parametric analysis of the Istanbul's ring road viaduct that is currently under construction within the Northern Marmara Highway project. The structure, due to its location on seismic prone areas is exposed to seismic loads of different strengths and different return periods. The study is focused on concrete bridge supports that are design to work in nonlinear range. The parametric study, conducted in MATLAB...
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Reliable Multi-Stage Optimization of Antennas for Multiple Performance Figures in Highly-Dimensional Parameter Spaces
PublicationDesign of modern antenna structures needs to account for multiple performance figures and geometrical constraints. Fulfillment of these calls for the development of complex topologies described by a large number of parameters. EM-driven tuning of such designs is mandatory yet immensely challenging. In this letter, a new framework for multi-stage design optimization of multi-dimensional antennas with respect to several performance...
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Identification of pollutants at the adjacent areas to the landfill site using two dimensional gas chromatography with mass spectrometry technique
PublicationMunicipal landfills are considered one of the main sources of air pollutants. In this article is presented the use of a modern analytical technique: a two dimensional gas chromatography to identify the substances in the atmospheric air near a waste processing plant located in Gdansk. In order to determine the types of substances occurring in different atmospheric conditions, the study was conducted from February to April 2016....
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Optimization of clamping stiffness during milling of high-dimensional structures with the use of techniques of experiment – aided virtual prototyping
PublicationThe subject of this paper is a method of searching for conditions of minimizing the vibration level of a tool-high dimensional flexible workpiece, at unchangeable technological parameters of the machining process. It depends on repeatable change of the values of the stiffness coefficients as soon as an optimal vibration state of the workpiece approaches. There are assessed the values of dominant ”peaks” in the frequency spectra...
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SPACIAL FORM DESIGN scale 1:1
e-Learning CoursesBrief description: The subject matter of the spatial form design classes is transformation. First, a black square becomes a flat graphic composition, which is then converted to color. The final stage of the transformation process is a spatial object, which is by assumption devoid of any utilitarian functions. Course content / classes schedule: 1. Graphic. The task is to create an achromatic flat composition in the contrast...
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Automatic music signal mixing system based on one-dimensional Wave-U-Net autoencoders
PublicationThe purpose of this paper is to show a music mixing system that is capable of automatically mixing separate raw recordings with good quality regardless of the music genre. This work recalls selected methods for automatic audio mixing first. Then, a novel deep model based on one-dimensional Wave-U-Net autoencoders is proposed for automatic music mixing. The model is trained on a custom-prepared database. Mixes created using the...
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Solvent effects on fluorescence properties of protochlorophyll and its derivatives with various porphyrin side chains
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Fluorescence Lifetimes Study of α-Tocopherol and Biological Prenylquinols in Organic Solvents and Model Membranes
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KMgF3:Eu2+ as a new fluorescence-based pressure sensor for diamond anvil cell experiments
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Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island film
PublicationOpisano fluorescencję barwnika CY5 połączonego z DNA w roztworze buforowym na wysepkach srebrowych.
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Concentration-Dependent Fluorescence Properties of Rhodamine 6G in Titanium Dioxide and Silicon Dioxide Nanolayers
PublicationWytworzono cienkie warstwy zawierające rodaminę 6G w dwutlenku tytanu (R6G/TiO2) i w dwutlenku krzemu (R6G/SiO2) stosując metodę zol-żel. Badane były dwa rodzaje matryc, w których domieszkowano barwnik rodamina 6G (R6G) o różnych koncentracjach. Został stwierdzony istotny wpływ koncentracji barwnika na widma absorpcji i emisji oraz na czasowo rozdzielone widma fluorescencji. W szczególności stwierdzono znacznie mniejszą zdolność...
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ENERGY ANALYSIS OF THE PROPULSION SHAFT FATIGUE PROCESS IN A ROTATING MECHANICAL SYSTEM PART III DIMENSIONAL ANALYSIS
PublicationThis article presents the third and last part of the problem of diagnosing the fatigue of marine propulsion shafts in terms of energy with the use of the action function, undertaken by the authors. Even the most perfect physical models of real objects, observed under laboratory conditions and developed based on the results of their research, cannot be useful in diagnostics without properly transferring the obtained results to the...
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Feasibility Study of Three-Phase Modular Converter for Dual-Purpose Application in DC and AC Microgrids
PublicationThe modern concept of a universal converter is intended as a power converter (PC) suitable for application in both dc or ac grids using the same external connectors. This novel family was recently proposed to allow easier integration of renewable energy sources and energy storage systems (ESSs), interfacing with dc/ac grids and/or loads with a minimum redundancy of power switches and passive elements. This kind of solution and...
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Feasibility Study of Three-Phase Modular Converter for Dual-Purpose Application in DC and AC Microgrids
PublicationThe modern concept of a universal converter is intended as a power converter suitable for application in both dc or ac grids using the same external connectors. This novel family was recently proposed to allow an easier integration of renewable energy sources and energy storage systems, interfacing with dc/ac grids and/or loads with a minimum redundancy of power switches and passive elements. This kind of solution and applications...
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A Generalized Framework Towards Structural Mechanics of Three-layered Composite Structures
PublicationThree-layered composite structures find a broad application. Increasingly, composites are being used whose layer thicknesses and material properties diverge strongly. In the perspective of structural mechanics, classical approaches to analysis fail at such extraordinary composites. Therefore, emphasis of the present approach is on arbitrary transverse shear rigidities and structural thicknesses of the individual layers. Therewith...
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Automatic audio signal mixing system based on one-dimensional Wave-U-Net autoencoders
PublicationThe purpose of this dissertation is to develop an automatic song mixing system that is capable of automatically mixing a song with good quality in any music genre. This work recalls first the audio signal processing methods used in audio mixing, and it describes selected methods for automatic audio mixing. Then, a novel architecture built based on one-dimensional Wave-U-Net autoencoders is proposed for automatic music mixing. Models...
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Morse decompositions for a two-dimensional discrete neuron model (low resolution)
Open Research DataThis dataset contains selected results of rigorous numerical computations conducted in the framework of the research described in the paper “Topological-numerical analysis of a two-dimensional discrete neuron model” by Paweł Pilarczyk, Justyna Signerska-Rynkowska and Grzegorz Graff. A preprint of this paper is available at https://doi.org/10.48550/arXiv.2209.03443.
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Morse decompositions for a two-dimensional discrete neuron model (limited range)
Open Research DataThis dataset contains selected results of rigorous numerical computations conducted in the framework of the research described in the paper “Topological-numerical analysis of a two-dimensional discrete neuron model” by Paweł Pilarczyk, Justyna Signerska-Rynkowska and Grzegorz Graff. A preprint of this paper is available at https://doi.org/10.48550/arXiv.2209.03443.
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Morse decompositions for a two-dimensional discrete neuron model (full range)
Open Research DataThis dataset contains selected results of rigorous numerical computations conducted in the framework of the research described in the paper “Topological-numerical analysis of a two-dimensional discrete neuron model” by Paweł Pilarczyk, Justyna Signerska-Rynkowska and Grzegorz Graff. A preprint of this paper is available at https://doi.org/10.48550/arXiv.2209.03443.
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The influence of dimensional accuracy on steel structures cost calculation
PublicationW referacie przedstawiono zależność pomiędzy wartością tolerancji a kosztami wytwarzania budowlanych konstrukcji stalowych. Opisano zasady klasyfikacji tolerancji prętowych konstrukcji stalowych z uwagi na porawny montaż konstrukcji wyrażony równaiem pętli montażowej. Przedstawiono podstawy analizy i syntezy tolerancji oraz zaproponowano nową formułę optymalizacji kosztów wytwarzania. Wskazano metody rozwiązania zadania optymalizacyjnego.
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Two-dimensional vertical analysis of dam-break flow
PublicationW artykule przedstawiono matematyczne modelowanie przepływów ze swobodną powierzchnią w kanałach otwartych. Do symulacji przepływu użyto dwuwymiarowy pionowy model RANS. Wchodzące w skład modelu równania Naviera-Stokesa rozwiązano stosując algorytm SIMPLE dla metody różnic skończonych. Do śledzenia zmian swobodnego zwierciadła wody zastosowano metodę MAC. Analizie poddano szybkozmienny przepływ po poziomym, gładkim dnie wywołany...
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Two-Boson Correlations in Various One-Dimensional Traps
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Two dimensional inverse method of turbomachinery stage design
PublicationW artykule przedstawion rys historyczny dotyczący modelu osiowo-symetrycznego w zastosowaniu do maszyn wirnikowych. Została sformułowana metoda rozwiązywania zadania odwrotnego polegaająca na znajdywaniu powierzchni będfącej obrazem łopatki w osiowo symetrycznym modelu maszyny wirnikowej. Zaprezentowano przykłady zastosowania tej metody w odniesieniu do łopatki kierowniczej turbiny, spreżarki promieniowej oraz pompo-turbiny.
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Informational entropy in simulation of one-dimensional random fields
PublicationEntropia ciągłego rozkładu prawdopodobieństwa jest zdefiniowana jako funkcja liczby węzłów skalarnego pola losowego. W przykładzie numerycznym przedstawiono propagacje entropii w zagadnieniu reakcji sprężystego wspornika przy losowym obciążeniu.