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Search results for: FUNGAL INFECTIONS
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Preliminary studies on the evolution of carbon assimilation abilities within Mucorales
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A novel source of the cardiac glycoside digoxin from the endophytic fungus Epicoccum nigrum : isolation, characterization, production enhancement by gamma irradiation mutagenesis and anticancer activity evaluation
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Harman and norharman, metabolites of the entomopathogenic fungus Conidiobolus coronatus (Entomophthorales), affect the serotonin levels and phagocytic activity of hemocytes, insect immunocompetent cells, in Galleria mellonella (Lepidoptera)
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Effect of aeration of antibiotic-loaded bone cement on its properties and bactericidal effectiveness
PublicationBACKGROUND: Antibiotic-loaded bone cements are now widely used in medicine. They are able to locally deliver antibiotic particles and they allow treat or protect against infection. It is assumed that the bactericidal effectiveness of bioactive bone cements depend on the parameters of its production. Hence, the aim of this study was to check the effect of aeration of bone cement before mixing the components on its properties as...
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Short-Term Outcomes of Percutaneous Trephination with a Platelet Rich Plasma Intrameniscal Injection for the Repair of Degenerative Meniscal Lesions. A Prospective, Randomized, Double-Blind, Parallel-Group, Placebo-Controlled Study
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Biofilm formation and its regulation in Staphylococcus aureus
PublicationInfections caused by the major human and animal pathogen, Staphylococcus aureus are becoming an important problem due to its emerging resistance mechanisms and variety of virulence factors. Biofilm formation is considered to be one of the most important factor attributed to high pathogenicity of S. aureus. Described in the literature methods used in identification of biofilm forming abilities of bacteria consist of phenotypic and...
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PCR test for Microsporum canis identification
PublicationMicrosporum canis, for which the natural hosts are cats and dogs, is the most prevalent zoophilic agent causing tinea capitis and tinea corporis in humans. We present here a diagnostic PCR test for M. canis, since its detection and species identification is relevant to the choice of treatment and to the understanding of a probable source of infection. An M. canis-specific PCR was evaluated using 130 clinical isolates of dermatophytes...
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Comparative analysis of mRNA transcripts of HT-29 cell line expressed in identical quantities for pathogenic E. coli strains UM146 and UM147 with control Escherichia coli Nissle 1917
PublicationAim of study was comparative analysis of mRNA transcripts of HT-29 cell line, expressed in identical quantities for the combination of pathogenic and non-pathogenic Escherichia coli strains. HT-29 confluent monolayers infection with two pathogenic E. coli strains UM146 and UM147 resulted in two sets of mRNA transcripts that were identical with RNA transcripts obtained for non-pathogenic one strain E. coli Nissle 1917. In this study...
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Determination of volatile fatty acids in municipal wastewater and leachates from sewage sludge allotments and compost piles using direct aqueous injection gas chromatography preceded by distillation based sample cleanup
PublicationOpracowano metodykę i wykorzystano ją do oznaczania lotnych kwasów tłuszczowych w zanieczyszczonych próbkach wodnych,takich jak ścieki komunalne, odcieki ze składowiska osadów ściekowych i pryzm kompostu, stosując metodę bezpośredniego dozowania rzeczywistej próbki wodnej lub jej rektyfikatu do chromatografu gazowego.
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Metal nanoparticles-assisted early diagnosis of diseases
PublicationEarly diagnosis is essential for the effective illness treatment, but traditional diagnostic approaches inevitably have major downsides. Recent advancements in nanoparticle-based biosensors have created new opportunities for accelerating diagnosis. High surface area, exceptional sensitivity, high specificity, and optical characteristics of metal and metal oxide nanoparticles have made it possible to detect a variety of health conditions...
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Molecular detection of Candida krusei
PublicationThe species identification of fungi belonging to genus Candida is an important issue as this genus becomes the emerging problem of nosocomial infections. As Candida krusei presents intrinsicresistance to the fluconazole that is the drug of the first choice in case of invasive candidiasis the PCR identifying the DNA of C. krusei was elaborated. The analytical sensitivity of the assay on spiked blood samples was estimated at 3-5...
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Artificial Intelligence in the Diagnosis of Onychomycosis—Literature Review
PublicationOnychomycosis is a common fungal nail infection that is difficult to diagnose due to its similarity to other nail conditions. Accurate identification is essential for effective treatment. The current gold standard methods include microscopic examination with potassium hydroxide, fungal cultures, and Periodic acid-Schiff biopsy staining. These conventional techniques, however, suffer from high turnover times, variable sensitivity,...
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A new assay for simultaneous identification and differentiation of Klebsiella oxytoca strains
PublicationBacterial strain typing, or identifying bacteria at the strain level, is particularly important for diagnosis, treatment, and epidemiological surveillance of bacterial infections. This is especially the case for bacteria exhibiting high levels of antibiotic resistance or virulence, and those involved in nosocomial or pandemic infections. Strain typing also has applications in studying bacterial population dynamics.
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Development of a solventless stir bar sorptive extraction/thermal desorption large volume injection capillary gas chromatographic-mass spectrometric method for ultra-trace determination of pyrethroids pesticides in river and tap water samples
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Mitigation effect of face shield to reduce SARS-CoV-2 airborne transmission risk: Preliminary simulations based on computed tomography
PublicationWe aimed to develop a model to quantitatively assess the potential effectiveness of face shield (visor) in reducing airborne transmission risk of the novel coronavirus SARS-CoV-2 during the current COVID-19 pandemic using the computational fluid dynamics (CFD) method. The studies with and without face shield in both an infected and healthy person have been considered in indoor environment simulation. In addition to the influence...
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Mitigation effect of face shield to reduce SARS-CoV-2 airborne transmission risk: Preliminary simulations based on computed tomography
PublicationWe aimed to develop a model to quantitatively assess the potential effectiveness of face shield (visor) in reducing airborne transmission risk of the novel coronavirus SARS-CoV-2 during the current COVID-19 pandemic using the computational fluid dynamics (CFD) method. The studies with and without face shield in both an infected and healthy person have been considered in indoor environment simulation. In addition to the influence...
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Biodiversity assessment of ascomycetes inhabiting Lobariella lichens in Andean cloud forests led to one new family, three new genera and 13 new species of lichenicolous fungi
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Versatility of putative aromatic aminotransferases from Candida albicans.
PublicationAmino acids constitute the key sources of nitrogen for growth of Candida albicans. In order to survive inside the host in different and rapidly changing environments, this fungus must be able to adapt via its expression of genes for amino acid metabolism. We analysed the ARO8, ARO9, YER152C, and BNA3 genes with regards to their role in the nutritional flexibility of C. albicans. CaAro8p is undoubtedly the most versatile enzyme...
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The Influence of Stretch Rod Speed on the Relationship between Preblown Bottle Aesthetic Quality and Final Blown Bottle Thickness Profile in Stretch Blow Molding from Preform Process
PublicationFrom a mechanical point of view, the aesthetic quality of preblown PET bottles and thickness profile of final blown PET bottles manufactured in ISBM process are determined by mechanical and thermal response of blown preforms. From the microscopic point of view the biggest influence on the mechanical and thermal properties of PET bottles have orientation and crystallization processes. From a technological point of view, the aesthetic...
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Chitosan-protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials.
PublicationAIMS: The development of technology for preparing chitosan-protein scaffolds loaded with lysostaphin, which potentially could be used as dressing for wound treatment and soft tissue infections caused by Staphylococcus aureus. METHODS AND RESULTS: The unique technology of chitosan solubilization using gaseous CO(2) instead of organic or inorganic acids was used for the incorporation of lysostaphin, the enzyme that exhibits bactericidal...
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Long range molecular dynamics study of interactions of the eukaryotic glucosamine-6-phosphate synthase with fructose-6-phosphate and UDP-GlcNAc
PublicationGlucosamine-6-phosphate synthase (EC 2.6.1.16) is responsible for catalysis of the first and practically irreversible step in hexosamine metabolism. The final product of this pathway, uridine 5 diphospho Nacetyl- d-glucosamine (UDP-GlcNAc), is an essential substrate for assembly of bacterial and fungal cell walls. Moreover, the enzyme is involved in phenomenon of hexosamine induced insulin resistance in type II diabetes, which...
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Effect of molar ratio [NCO]/[OH] groups during prepolymer chains extending step on the morphology and selected mechanical properties of final bio‐based thermoplastic poly(ether‐urethane) materials
PublicationThe main aim of this work was to investigate the effect of [NCO]/[OH] molar ratio used during the prepolymer chain extending step (with bio‐based diols) on the chemical structure, and thermomechanical and mechanical properties of thermoplastic poly(ether‐urethane)s. Thermoplastic poly(ether‐urethane)s were obtained from bio‐based polyol (polytrimethylene ether glycol), bio‐based glycol (1,4‐butanediol or 1,3‐propanediol), and 4,4'diphenylmethane...
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Clinical studies of detecting COVID-19 from exhaled breath with electronic nose
PublicationThe COVID‑19 pandemic has attracted numerous research studies because of its impact on society and the economy. The pandemic has led to progress in the development of diagnostic methods, utilizing the polymerase chain reaction (PCR) as the gold standard for coronavirus SARS‑CoV‑2 detection. Numerous tests can be used at home within 15 min or so but of with lower accuracy than PCR. There is still a need for point‑of‑care tests available...
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New PCR test for detection of Candida glabrata based on the molecular target chosen by the RAPD technique
PublicationRapid, reliable diagnosis is a necessary condition for the successful treatment of infections. Such diagnostic assays are continually being developed. #e paper presents a method for selecting the molecular target for PCR-based diagnostics based on the comparison of RAPD patterns. A sequence encoding Candida glabrata CBS138 hypothetical protein was selected. The limit of detection for PCR and real-time PCR reactions with DNA extracted...
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Inhibitors of amino acids biosynthesis as antifungal agents
PublicationFungal microorganisms, including the human pathogenic yeast and filamentous fungi, are able to synthesize all proteinogenic amino acids, including nine that are essential for humans. A number of enzymes catalyzing particular steps of human-essential amino acid biosynthesis are fungi specific. Numerous studies have shown that auxotrophic mutants of human pathogenic fungi impaired in biosynthesis of particular amino acids exhibit...
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Biofilm bakteryjny uropatogennych szczepów E. coli Dr+ jako czynnik indukujący przewlekłość zakażeń dróg moczowych ograniczający ich leczenie
PublicationZakażenia dróg moczowych (ZUM) stanowią jedne z najczęściej występujących infekcji bakteryjnych, dotykających każdego roku miliony osób na świecie. Problematyka tych zakażeń wynika z ich przewlekłości i nawrotów, pomimo stosowania terapii antybiotykowej oraz ciągle wzrastającej lekooporności uropatogenów je wywołujących. Dominującym czynnikiem etiologicznym ZUM są uropatogenne szczepy E. coli (UPECs), wykazujące zdolność do adhezji,...
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Examination of cyp51A and cyp51B expression level of the first Polish azole resistant clinical Aspergillus fumigatus isolate
PublicationAspergillus fumigatus is one of the most prevalent airborne fungal pathogens causing infections worldwide. Most A. fumigatus strains are susceptible to azoles, which are administered as the first line therapeutics. However, during last decade the acquired resistance to triazoles by these species has been described. There is a number of publications concerning the examination of clinical A. fumigatus strains from different countries,...
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Experimental study on polymer mass used to repair damaged structures
PublicationA new method of repairing damaged structures by injecting the cracks with specially designed polymer mass (flexible two-component grout based on polyurethane resin) has been recently proposed. The technique is mainly dedicated to damaged masonries, especially historical structures where minimum intervention is permitted. The cracks are filled with the special injection, forming the flexible joints bonding the disrupted structural...
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The New Klebsiella pneumoniae ST152 Variants with Hypermucoviscous Phenotype Isolated from Renal Transplant Recipients with Asymptomatic Bacteriuria-Genetic Characteristics by WGS.
PublicationKlebsiella pneumoniae (Kp) is one of the most important etiological factors of urinary tract infections in renal transplant (RTx) recipients. We described the antimicrobial susceptibility phenotypes and genomic features of two hypermucoviscous (HM) Kp isolates recovered from RTx recipients with asymptomatic bacteriuria (ABU). Using whole genome sequencing (WGS) data, we showed that the strains belong to the ST152 lineage with the...
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Optical method supported by machine learning for dynamics of C‐reactive protein concentrations changes detection in biological matrix samples
PublicationIn this article we present the novel spectroscopy method supported with machine learning for real-time detection of infectious agents in wastewater. In the case of infectious diseases, wastewater monitoring can be used to detect the presence of inflammation biomarkers, such as the proposed C-reactive protein, for monitoring inflammatory conditions and mass screening during epidemics for early detection in communities of concern,...
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Fabrication methods of smart composite coatings - review
PublicationPostoperative bacterial infections are one of the main reasons for unsuccessful implantation of long-term implants. The development of bacterial infection requires antibiotic therapy, in extreme cases a reimplantation procedure is necessary. In order to provide materials for implants with antibacterial properties, they are subjected to modifications to create a coating that will release the drug substance, when the inflammation...
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Antibiotic-sterol interactions provide insight into the selectivity of natural aromatic analogues of amphotericin B and their photoisomers
PublicationAromatic heptaene macrolides (AHMs) belong to the group of polyene macrolide antifungal antibiotics. Members of this group were the first to be used in the treatment of systemic fungal infections. Amphotericin B (AmB), a non-aromatic representative of heptaene macrolides, is of significant clinical importance in the treatment of internal mycoses. It includes the all-trans heptaene chromophore, whereas the native AHMs contain two...
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Klimatyzacja i wentylacja szpitalnych zespołów operacyjnych
PublicationUtrzymywanie czystości i higieny w obiektach ochrony zdrowia jest nieodłącznym elementem troski o zdrowie pacjentów i personelu medycznego. W szpitalu codziennie nawet kilkudziesięciu pacjentów może być poddawanych zabiegom operacyjnym, czasem skomplikowanym, trwającym długo i wymagającym dużego wysiłku od personelu medycznego i lekarzy operatorów. Podczas zabiegów inwazyjnych – szczególnie trudnych i długotrwałych – poziom emisji...
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Peptidoglycan hydrolases - potential weapons against Staphylococcus aureus
PublicationBacteria of the genus Staphylococcus are common pathogens responsible for a broad spectrum of human and animal infections and belong to most important etiological factors causing food poisoning. Because of rapid increase in prevalence of isolation of staphylococci resistant to many antibiotics, there is an urgent need for development of new alternative chemotherapeutics. A number of studies have recently demonstrated the strong...
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The Latest Advances in Wireless Communication in Aviation, Wind Turbines and Bridges
PublicationPresent-day technologies used in SHM (Structural Health Monitoring) systems in many implementations are based on wireless sensor networks (WSN). In the context of the continuous development of these systems, the costs of the elements that form the monitoring system are decreasing. In this situation, the challenge is to select the optimal number of sensors and the network architecture, depending on the wireless system’s other parameters...
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New recombinant chimeric antigens, P35-MAG1, MIC1-ROP1, and MAG1-ROP1, for the serodiagnosis of human toxoplasmosis
PublicationThe aim of the study was to evaluate the usefulness of 3 chimeric Toxoplasma gondii antigens, P35-MAG1,MIC1-ROP1 andMAG1-ROP1, in the serodiagnosis of an acute toxoplasmosis in humans. Proteins were produced as fusion proteins containing His tags ends and then further purified by metal affinity chromatography. Their application for the diagnosis of recently acquired T. gondii infection was tested in IgG and IgM enzyme-linked immunosorbent...
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A new human IgG avidity test, using mixtures of recombinant antigens (rROP1, rSAG2, rGRA6), for the diagnosis of difficult-to-identify phases of toxoplasmosis
PublicationThe preliminary diagnostic utility of two mixtures of Toxoplasma gondii recombinant antigens (rROP1+rSAG2 and rROP1+rGRA6) in IgG ELISA and IgG avidity test has been evaluated. A total of 173 serum samples from patients with toxoplasmosis and seronegative people were examined. The sensitivity of IgG ELISA for rROP1+rSAG2 and rROP1+rGRA6 was 91.1% and 76.7%, respectively, while the reactivity for sera from patients where acute toxoplasmosis...
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Characterization of d-xylose reductase, XyrB, from Aspergillus niger
PublicationD-xylose reductase is a member of the aldo-keto reductase family, and is involved in D-xylose and L-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second D-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert D-xylose and L-arabinose, and it has the highest affinity for these sugars...
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Discrimination of hospital isolates of Acinetobacter baumannii using repeated sequences and whole genome alignment differential analysis
PublicationAn optimized method for bacterial strain differentiation, based on combination of Repeated Sequences and Whole Genome Alignment Differential Analysis (RS&WGADA), is presented in this report. In this analysis, 51 Acinetobacter baumannii multidrug-resistance strains from one hospital environment and patients from 14 hospital wards were classified on the basis of polymorphisms of repeated sequences located in CRISPR region, variation...
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Wpływ parametrów strugi paliwa wtryskiwanego do cylindra silnika okrętowego na początkową fazę rozpylania
PublicationPrzedmiotem rozprawy jest analiza procesu wtrysku oleju napędowego do cylindra silnika okrętowego z zapłonem samoczynnym i bezpośrednim wtryskiem paliwa. Analiza tego zagadnienia została przeprowadzona w aspekcie wpływu makro parametrów strugi na początkową fazę rozpylania oleju napędowego. Celem niniejszej pracy jest określenie wpływu współczynnika L/D (długość otworu/średnica) otworu wylotowego wtryskiwacza na zmianę charakterystyki...
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Structure and evolution of the 4-helix bundle domain of Zuotin, a J-domain protein co-chaperone of Hsp70
PublicationThe J-domain protein Zuotin is a multi-domain eukaryotic Hsp70 co-chaperone. Though it is primarily ribosome-associated, positioned at the exit of the 60S subunit tunnel where it promotes folding of nascent polypeptide chains, Zuotin also has off-ribosome functions. Domains of Zuotin needed for 60S association and interaction with Hsp70 are conserved in eukaryotes. However, whether the 4-helix bundle (4HB) domain is conserved remains...
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Structure-function analysis of decay-accelerating factor: identification ofresidues important for binding of the Escherichia coli Dr adhesin and complement regulation
PublicationZidentyfikowano ważne dla wiązania adhezyny Dr uropatogennych Escherichiacoli reszty aminokwasowe w ludzkim receptorze DAF SCR-3.
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Studies of Streptococcus anginosus Virulence in Dictyostelium discoideum and Galleria mellonella Models
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Chimeric Dr fimbriae with a Herpes Simplex virus type 1 epitope as a model for a recombinant vaccine.
PublicationBiałko adhezyny DraE uropatogennych szczepów E. coli Dr+ zostało wykorzystane jako nośnik 11a a determinanty antygenowej glikoproteiny D wirusa HSV ty-pu 1. Sekwencja kodująca epitop HSV została wklonowana do genu draE w obrębie regionu N- terminalnego eksponowanej powierzchniowo domeny 2. Ekspresjachimerycznych białek DraE-HSV została potwierdzona za pomocą mikroskopii immunofluorescencyjnej i elektronowej.
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Biomolecular influenza virus detection based on the electrochemical impedance spectroscopy using the nanocrystalline boron-doped diamond electrodes with covalently bound antibodies
PublicationNew rapid pathogen detection methods with improved cost-effectiveness and efficiency are currently in the focus of the scientists from all over the world. Based on the experiences from the rapid spread of the influenza virus pandemic in 2009 it is clear that the development of the system for early diagnosis of this infection is essential. The crucial stage of the treatment is the detection of the viral infection during its initial...
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Wieloletnie obserwacje soplówki jeżowatej Hericium erinaceum (Bull.: Fr.) Pers. (macromycetes) na stanowisku w Lasach Oliwskich (Trójmiejski Park Krajobrazowy) = Long-term observation of bearded tooth fungus Hericium erinaceum (Bull.: Fr.) Pers. (macromycetes) in Oliwskie Forests (Trójmiejski Landscape Park)
PublicationW pracy omówiono soplówkę jeżowatą oraz podano miejsca jej występowania w kraju i za granicą. Szczegółowo opisano stanowisko soplówki jeżowatej w Lasach Oliwskish w Trómiejskim Parku Krajobrazowym.
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Modification of current-voltage characteristics of planar organic systems by nm-thick interlayer
PublicationEffect of nm-thick interlayer of copper phthalocyanine (CuPc) or perylene dye (MePTCDI) on current-voltage characteristics of planar organic systems is discussed in the work. The MePTCDI layer strongly limits injection of holes from ITO into CuPc, while CuPc limits injection of electrons from ITO into MePTCDI. In the systems with CuPc/MePTCDI heterojunction an explicit photovoltaic effect occurs.
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Differences in sequences between HBV-relaxed circular DNA and covalently closed circular DNA
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Parasites of the digestive tract of sheep and goats from organic farms in Western Pomerania, Poland
PublicationThe aim of the study was to determine the prevalence and intensity of parasite infection of the digestive tract in sheep and goats from the West Pomerania region following anti-parasite treatment. Feces were freely collected from sheep and goats kept on organic farms and subjected to analysis by the Willis-Schlaf and McMaster’s flotation methods. The mean extensity of infection by gastrointestinal parasites...
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Intencje przedsiębiorcze studentów – analiza międzynarodowa oparta na rozszerzonym modelu Ajzena
PublicationPoszukiwanie czynników wpływających na rozpoczęcie działalności gospodarczej doczekało się opisu w wielu publikacjach. Nie ma wątpliwości, że czynnikiem kluczowym w procesie przedsiębiorczym są intencje, a dzięki pracom I. Ajzena wiadomo także, jakie grupy czynników wpływają na intencje. Jednak jego teoria planowanego zachowania wydaje się być niewystarczająca. Celem rozdziału było opracowanie propozycji rozszerzenia teorii Ajzena....