displaying 1000 best results Help
Search results for: motor proteins
-
Monitoring of lysozyme thermal denaturation by volumetric measurements and nanoDSF technique in the presence of N-butylurea
PublicationThe results of thermal studies of denaturation of hen egg white lysozyme (HEWL) in water and an aqueous solution of N-butylurea (BU) are presented. High-precision densimetric measurements were used to characterize and analyze the changes of the specific volume, v, during temperature elevation. The temperature of the midpoint of protein denaturation was also determined by nanoDSF technique (differential scanning fluorimetry). The...
-
Suppressors of lapC mutation identify new regulators of LpxC, which mediates the first committed step in lipopolysaccharide biosynthesis
PublicationGram-negative bacteria, such as Escherichia coli, are characterized by an asymmetric outer membrane (OM) with lipopolysaccharide (LPS) located in the outer leaflet and phospholipids facing the inner leaflet. E. coli recruits LPS assembly proteins LapB, LapC and LapD in concert with FtsH protease to ensure a balanced biosynthesis of LPS and phospholipids. We recently reported that bacteria either lacking the periplasmic domain of...
-
Application of human bile salts for in vitro digestion models
PublicationIn this study, experimental in vitro models simulating the environment of the human gastrointestinal tract were used to assess the impact of physiological surfactants, such as bile salts, on the kinetics of digestion. Bile salts are biosurfactants synthesised in the liver and secreted together with bile into the small intestine. There are many reports on the role of bile salts in lipolysis, but the knowledge of their influence...
-
Theoretical and Actual Feed Per Tooth During Wood Sawing on an Optimizing Cross-Cut Saw
PublicationThe work presents the geometries of circular saw teeth, whose task is to divide the cross-cut of the cutting layer. For efficient machining on a cross-cut saw with high feed speed values (about 1 ms−1) maximal theoretical feeds per tooth are determined. These values were compared with the actual values determined during transverse cutting of pine wood on a special test stands in industrial conditions. In the experiment the rotational...
-
Simulation of signal acquisition from a rotary flowmeter
Open Research DataThe dataset contains results of simulation measuring the flow of homogeneous substances by rotational flow meter: a moment of impulse at the output of flow meter, time between successive pulses, number of pulses counted from standard generator and relative error of measurement.
-
Geometric analogue of holographic reduced representation
PublicationHolographic reduced representations (HRRs) are distributed representations of cognitive structuresbased on superpositions of convolution-bound n-tuples. Restricting HRRs to n-tuples consisting of 1,one reinterprets the variable binding as a representation of the additive group of binary n-tupleswith addition modulo 2. Since convolutions are not defined for vectors, the HRRs cannot be directlyassociated with geometric structures....
-
Aravind Ayyolath
PeopleI am interested in the biology of bacterial stress response and its growth under stress conditions. Our lab focuses on two aspects; protein folding and lipopolysaccharide biosynthesis and we address them with Escherichia coli models. Our recent studies have provided more evidence regarding several genes involved in the survival of bacteria under stress. I am also interested in disease biology and also in identifying proper therapeutic...
-
Bioactivities of Phenolic Compounds from Kiwifruit and Persimmon
PublicationFruit used in the common human diet in general, and kiwifruit and persimmon particularly, displays health properties in the prevention of heart disease. This study describes a combination of bioactivity, multivariate data analyses and fluorescence measurements for the differentiating of kiwifruit and persimmon, their quenching and antioxidant properties. The metabolic differences are shown, as well in the results of bioactivities...
-
Antimicrobial Activity of Selected Antimicrobial Peptides Against Planktonic Culture and Biofilm of Acinetobacter baumannii
Publication -
Anticandidal Activity of Omiganan and Its Retro Analog Alone and in Combination with Fluconazole
Publication -
Alanine Scanning Studies of the Antimicrobial Peptide Aurein 1.2
Publication -
Probiotic potential of Bacillus Isolates from Polish Bee Pollen and Bee Bread
PublicationThe main goal of this study was the evaluation of the probiotic potential of 10 Bacillus spp. strains isolated from 5 bee bread and 3 bee pollen samples. The antagonistic interaction with Staphylococcus aureus and Escherichia coli was a primary criterion for the preliminary selection of the isolates. Three out of ten strains—PY2.3 (isolated from pollen), BP20.15 and BB10.1 (both isolated from bee bread)—were found to be possible...
-
Attenuation of Hyperglycemia in Diabetic Rats Assisted by Immobilized Probiotic in Sodium Alginate
PublicationDiabetes mellitus type 2 (DM2) is the most common chronic disease worldwide, characterized mainly by increased glucose concentration in the blood and affecting several organs’ functionality. The daily consumption of probiotic bacteria can help control diabetes and reduce the damage caused. Cell immobilization techniques are a powerful tool that provides physical cell protection to such probiotic bacteria against gastrointestinal...
-
Sensorless Multiscalar Control of Five-Phase Induction Machine with Inverter Output Filter
PublicationThe paper presents a complete solution for speed sensorless control system for five-phase induction motor with voltage inverter, LC filter and nonlinear control of combined fundamental and third harmonic flux distribution. The control principle, also known as multiscalar control, nonlinear control or natural variables control, is based on a use of properly selected scalar variables in control feedback to linearize controlled system....
-
Arsalan Muhammad Soomar Doctoral Student
PeopleHi, I'm Arsalan Muhammad Soomar, an Electrical Engineer. I received my Master's and Bachelor's Degree in the field of Electrical Engineering from Mehran University of Engineering and Technology, Jamshoro, Sindh, Pakistan. Currently enrolled as a Doctoral student at the Gdansk University of Technology, Gdansk, Poland. Also worked in Yellowlite. INC, Ohio as a Solar Design Engineer. HEADLINE Currently Enrolled as a Doctoral...
-
Molecular simulation study of cooperativity in hydrophobic association
Publication -
AmiP from hyperthermophilic Thermus parvatiensis prophage is a thermoactive and ultrathermostable peptidoglycan lytic amidase
Publication -
A mobile loop order–disorder transition modulates the speed of chaperonin cycling
PublicationMolecular machines order and disorder polypeptides as they form and dissolve large intermolecular interfaces, but the biological significance of coupled ordering and binding has been established in few, if any, macromolecular systems. The ordering and binding of GroES co-chaperonin mobile loops accompany an ATP-dependent conformational change in the GroEL chaperonin that promotes client protein folding. Following ATP hydrolysis,...
-
Novel Nonlinear High Order Technologies for Damage Diagnosis of Complex Assets
PublicationFor the first time worldwide, innovative techniques, generic non-linear higher-order unnormalized cross-correlations of spectral moduli, for the diagnosis of complex assets, are proposed. The normalization of the proposed techniques is based on the absolute central moments, that have been proposed and widely investigated in mathematical works. The existing higher-order, crosscovariances of complex spectral components are not sufficiently...
-
Wybrane metody diagnostyki łożysk silników indukcyjnych oparte o pomiar prądu
PublicationW artykule zawarto przegląd wybranych metod diagnostyki łożysk silnika indukcyjnego, bazujących na pomiarach prądu zasilającego. Jedno z nowych rozwiązań przetwarzania sygnałów zostało zaadaptowane przez autorów do stosowanego przez nich systemu diagnostycznego. Wyniki wstępnych badań symulacyjnych zweryfikowanych badaniami na rzeczywistym obiekcie wskazują, że rozwiązanie to ułatwia diagnozowanie. Autorzy zamierzają prowadzić...
-
Metoda diagnostyki łożysk na podstawie analizy przebiegów prądu i napięcia zasilającego silnik indukcyjny.
PublicationW niniejszej monografii przedstawiono oryginalne podejście i realizację diagnostyki łożysk silników indukcyjnych przy użyciu metody opartej na pomiarach i analizie iloczynu wartości chwilowych prądu i napięcia zasilającego maszyny. Uzyskane wyniki badań eksperymentalnych na obiektach rzeczywistych okazały się zbieżne z wynikami badań symulacyjnych i potwierdziły, że powstanie uszkodzeń łożysk w silniku skutkuje pojawieniem się...
-
Two-step mechanism of J-domain action in driving Hsp70 function
PublicationJ-domain proteins (JDPs), obligatory Hsp70 cochaperones, play critical roles in protein homeostasis. They promote key allosteric transitions that stabilize Hsp70 interaction with substrate polypeptides upon hydrolysis of its bound ATP. Although a recent crystal structure revealed the physical mode of interaction between a J-domain and an Hsp70, the structural and dynamic consequences of J-domain action once bound and how Hsp70s...
-
Identification of Substrates of Cytoplasmic Peptidyl-Prolyl Cis/Trans Isomerases and Their Collective Essentiality in Escherichia Coli
PublicationProtein folding often requires molecular chaperones and folding catalysts, such as peptidyl-prolyl cis/trans isomerases (PPIs). The Escherichia coli cytoplasm contains six well-known PPIs, although a requirement of their PPIase activity, the identity of their substrates and relative enzymatic contribution is unknown. Thus, strains lacking all periplasmic and one of the cytoplasmic PPIs were constructed. Measurement of their PPIase...
-
Voltage fluctuations on the main switchgear of the industrial power system supplying the rolling mill motors_part_2
Open Research DataThe dataset presents the voltage waveforms on the bus bars of the main switchgear of the industrial power network for the supply of rolling mills. The data was recorded during an experiment whose purpose was to determine a level of short-term and long-term flicker caused by voltage fluctuations. In the virtual application of flickermeter, a hardware...
-
Fault-tolerant performance of the novel five-phase doubly-fed induction generator
PublicationThe article presents the concept of a new design of a multi-phase doubly-fed induction generator (DFIG). The innovative design approach uses a five-phase power supply from the rotor side of the generator with a three-phase classic stator power supply. Modern three-phase doubly-fed induction generators are the dominant choice for Wind Energy Conversion Systems (WECS). Solutions of this type are sensitive to the loss of at least...
-
Przegląd rodzajów chiralnych faz stacjonarnych oraz możliwości ich zastosowań w chromatografii cieczowej
PublicationChromatograficzne rozdzielanie związków optycznie czynnych ma ogromne znaczenie nie tylko w przemyśle farmaceutycznym, ale i agrochemicznym, a także w badaniach naukowych różnego rodzaju. W niniejszym opracowaniu scharakteryzowano komercyjnie dostępne chiralne fazy stacjonarne na bazie, cyklodekstryn, polisacharydów, makrocyklicznych antybiotyków, eterów koronowych, a także fazy proteinowe, ligandowymienne, jonowymienne oraz fazy...
-
MutS3: a MutS homologue of unknown biological function
PublicationThe homologues of MutS proteins are widespread among both Prokaryotes and Eukaryotes. MutS designated as MutS1 is a part of MMR (mismatch repair) system which is responsible for removal of mispaired bases and small insertion/deletion loops in DNA. Initially, the only MutS homologues known were those engaged in mismatch repair and these were later designated as MutS1. Subsequently, the MutS2 homologue was distinguished. MutS2 does...
-
Polish Regions in Implementation of the Lisbon Strategy
PublicationPolskie regiony w gospodarce opartej na wiedzy. Innowacje jako motor rozwoju regionalnego. Rola regionalnych strategii innowacji (RIS). Fundamentem RIS są technologie innowacyjne i kompleksy naukowo-badawcze. Warunkiem jest rozwój społeczeństwa wiedzy.
-
Pathological and physiological high-frequency oscillations in focal human epilepsy
PublicationHigh-frequency oscillations (HFO; gamma: 40-100 Hz, ripples: 100-200 Hz, and fast ripples: 250-500 Hz) have been widely studied in health and disease. These phenomena may serve as biomarkers for epileptic brain; however, a means of differentiating between pathological and normal physiological HFO is essential. We categorized task-induced physiological HFO during periods of HFO induced by a visual or motor task by measuring frequency,...
-
Funkcjonowanie zespołów napędu hydraulicznego maszyn w niskich temperaturach otoczenia
PublicationW pracy przedstawiono cztery warianty klasyfikacji rozruchów oziębionego napędu hydraulicznego. Pierwsza sytuacja dotyczy zagadnienia, w którym wszystkie zespoły układu napędowego wraz z olejem mają taką samą niską temperaturę podczas rozruchu. Pozostałe warianty odnoszą się do rozruchu w warunkach szoku termicznego wywołanych zasilaniem ciepłym czynnikiem roboczym oziębionych zespołów hydraulicznych. Rozruch zespołów...
-
Molecular dynamics study on the role of solvation water in the adsorption of hyperactive AFP to the ice surface
PublicationUsing computer simulations, the early stages of the adsorption of the CfAFP molecule to the ice surface were analyzed. We found that the ice and the protein interact at least as early as when the protein is about 1 nm away from the ice surface. These interactions are mediated by interfacial solvation water and are possible thanks to the structural ordering of the solvent. This ordering leads to positional preference of the protein...
-
Research on Linear Actuators for Active Foil Bearings
PublicationActive foil bearings are a kind of gas foil bearing. They contain actuators which allow for modification of the bearing sleeve size and the shape of the lubrication gap. Rotor vibrations can be actively controlled by these changes. It is possible, among other things, to reduce the starting torque, control the vibration amplitude at different speeds and improve operational safety. Prototypes of active foil bearings are being developed...
-
Small regulatory bacterial RNAs regulating the envelope stress response
PublicationMost bacteria encode a large repertoire of RNA-based regulatory mechanisms. Recent discoveries have revealed that the expression of many genes is controlled by a plethora of base-pairing noncoding small regulatory RNAs (sRNAs), regulatory RNA-binding proteins and RNA-degrading enzymes. Some of these RNA-based regulated processes respond to stress conditions and are involved in the maintenance of cellular homeostasis. They achieve...
-
Determination of long-chain aldehydes using a novel quartz crystal microbalance sensor based on a biomimetic peptide
PublicationThere is an increasingly popular trend aimed at improvement of fundamental metrological parameters of sensors via implementation of materials mimicking biological olfactory systems. This study presents investigation on usefulness of the peptide mimicking HarmOBP7 region as a receptor element of the piezoelectric sensor for selective analysis of long-chain aldehydes. Identification of odorant binding proteins creates new possibilities...
-
Analytical modeling of electric drives for vehicle traction control systems
PublicationWheel-torque control bandwidth in vehicle dynamics model is crucial in evaluating optimal tracking ability of tire slip and in analysis of control system stability. Electric drives’ control dynamics are influenced by timing of digital control events, e.g. by sampling instant of motor currents. Reflecting this timing in drive analytical models is therefore important for analysis of vehicle traction systems. The paper considers five...
-
Impact of Low Switching-to-Fundamental Frequency Ratio on Predictive Current Control of PMSM: A simulation study
PublicationPredictive current control algorithms for permanent magnet synchronous (PMSM) drives rely on an assumption that within short intervals motor currents can be approximated with linear functions. This approximation may result either from discretizing the motor model or from simplifications applied to the continuous-time model. As the linear current approximation has been recognized as inaccurate in case when the drive operates with...
-
IDENTIFICATION OF THE INTERFECE OF LATERAL SLIDE BEARINGS
PublicationIn the paper, the model of slide bearings of motor engine, the metodology of research on this type of bearings and its results are presented measurement standpoint consisting of main elements of Briggs & Stratton 550 serie 10 T 802 model piston motor engine driven by three phase elektric engine is introduced . On the measurement standpoint are measurements were done, needed for specification...
-
Współczynniki strat energetycznych w elementach napędu hydrostatycznego
PublicationW każdym układzie napędowym należy zastąpić obraz przepływu mocy opisany dotychczas powszechnie stosowanym wykresem Sankey’a spadku mocy zgodnego z kierunkiem przepływu mocy proponowanym przez autora wykresem wzrostu mocy w układzie przeciwnego do kierunku przepływu mocy. Należy więc opisywać straty i sprawność energetyczną a także pole pracy każdego silnika i układu napędowego jako zależne od wielkości fizycznych niezależnych...
-
Chemical Aspects of Biological Activity of Isothiocyanates and Indoles, the Products of Glucosinolate Decomposition
PublicationThere is growing evidence that cancer chemoprevention employing natural, bioactive compounds may halt or at least slow down the different stages of carcinogenesis. A particularly advantageous effect is attributed to derivatives of sulfur-organic phytochemicals, such as glucosinolates (GLs) synthesized mainly in Brassicaceae plant family. GLs are hydrolysed enzymatically to bioactive isothiocyanates (ITC) and indoles, which exhibit strong...
-
Neutral point balancing technique for 3-level neutral point clamped converter with servo system
PublicationNeutral point voltage drift compensation technique in 3-level NPC multilevel converter and servo system is described in the paper. Analytical expressions are obtained for power subsystem elements parameters of servo drive system. Simulation of servo system, based on PMSM motor with 3-level NPC converter is considered.
-
Regulated assembly of lipopolysaccharide and sensing of its alterations in Escherichia coli
PublicationThis thesis describes mechanism of the regulation of the transcription of the rpoE gene encoding an essential RNA polymerase subunit in Escherichia coli. The RpoE regulates extracytoplasmic stress response regulon and is required to initiate transcription of genes, whose products are involvedin the folding of periplasmic proteins and synthesis and transport of outer membrane components. The transcriptional regulation of the rpoE...
-
Mechanochemical Energy Transduction during the Main Rotary Step in the Synthesis Cycle of F1-ATPase
PublicationF1-ATPase is a highly efficient molecular motor that can synthesize ATP driven by a mechanical torque. Its ability to function reversibly in either direction requires tight mechanochemical coupling between the catalytic domain and the rotating central shaft, as well as temporal control of substrate binding and product release. Despite great efforts and significant progress, the molecular details of this synchronized and fine-tuned...
-
An electric ring thruster as auxiliary manoeuvring propulsion system for watercraft - construction analysis
PublicationThe reported project aimed at examining properties and purposefulness of use of modern electromagneticbearings for a screw propeller in a prototype version of a synchronous ring motor with rare earths magnets.Bearings of this type generate electromagnetic forces which keep the rotor in a state of levitation. Therotating machine with magnetic bearings can work in any environment which reveals diamagnetic properties(air, vacuum,...
-
Running characteristics of aerodynamic bearing with self-lifting capability at low rotational speed
PublicationAn aerodynamic journal bearing that is capable of self-generating squeeze-film pressure is presented and its dynamic characteristics investigated numerically and experimentally. A numerical method based on a time marching static model was applied to assess the orbit trajectory path of the rotor upon a perturbation. Experimental results were obtained to validate the effect of the self- generated squeeze-film pressure on the stability...
-
Income obtained according to particular rates only by taxpayers conducting non-agricultural business activity 2015
Open Research DataTaxation in the form of a lump sum on recorded revenues does not apply to certain types of activity, regardless of their size. We are talking about taxpayers who achieve revenue in whole or in part, among others from on account of running pharmacies, activities in the field of buying and selling foreign exchange values, activities in the field of trade...
-
Fibrillar aggregates in powdered milk
PublicationThis research paper addresses the hypothesis that powdered milk may contain amyloid fibrils. Amyloids are fibrillar aggregates of proteins. Up to this time, research on the presence of amyloids in food products are scarce. To check the hypothesis we performed thioflavin T fluorescence assay, X-ray powder diffraction, atomic force microscopy and fluorescence microscopy imaging. Our preliminary results show that commercially available...
-
Overproduction of CaMet15p native and His-tag versions.
Open Research DataEnzyme of fungal L-methionine biosynthetic pathway: O-acetylhomoserine sulfhydrylase (Met15p) could be exploited as molecular target for antifungal chemotherapy. The goal of the study was to obtain conditions optimal for protein production with the use of prokaryotic cells. The constructed expression plasmids, designed in three different versions: encoding...
-
Overproduction of CaStr2p native and His-tag versions.
Open Research DataEnzyme of fungal L-methionine biosynthetic pathway: cystathionine-γ-synthase (CaStr2p) could be exploited as molecular target for antifungal chemotherapy. The goal of the study was to obtain conditions optimal for protein production with the use of prokaryotic cells. The constructed expression plasmids, designed in three different versions: encoding...
-
Overproduction of homoserine O-acetyltransferase (CaMet2p) native and His-tag versions.
Open Research DataEnzyme of fungal L-methionine biosynthetic pathway: homoserine O-acetyltransferase (Met2p) could be exploited as molecular target for antifungal chemotherapy. The goal of the study was to obtain conditions optimal for protein production with the use of prokaryotic cells. The constructed expression plasmids, designed in three different versions: encoding...
-
Ryszard Strzelecki prof. dr hab. inż.
People