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Wyniki wyszukiwania dla: antibiotic resistance genes
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The role of glucuronidation in drug resistance
PublikacjaThe final therapeutic effect of a drug candidate, which is directed to a specific molecular target strongly depends on its absorption, distribution, metabolism and excretion (ADME). The disruption of at least one element of ADME may result in serious drug resistance. In this work we described the role of one element of this resistance: phase II metabolism with UDP-glucuronosyltransferases (UGTs). UGT function is the transformation...
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Environmental Phage-Based Cocktail and Antibiotic Combination Effects onAcinetobacter baumanniiBiofilm in a Human Urine Model
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Transcriptional activity of epigenetic remodeling genes declines in keratinocytes after in vitro expansion
PublikacjaPURPOSE: In vitro expansion is an invaluable method to obtain keratinocytes in amounts necessary for effective transplantation therapies. In vitro cell culturing provokes questions concerning potential epigenetic alterations occurring in expanded cells in the context of usefulness for transplantation and safety. The purpose of this study was to investigate as to whether keratinocyte expansion is associated with changes in the activity...
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Transport deficiency is the molecular basis of Candida albicans resistance to antifungal oligopeptides
Publikacja(FMDP), an inhibitor of glucosamine-6-phosphate synthase, exhibited growth inhibitory activity against Candida albicans, with minimal inhibitory concentration values in the 0.05–50 mg/L range. Uptake by the peptide permeases was found to be the main factor limiting an anticandidal activity of these compounds. Di- and tripeptide containing FMDP (F2 and F3) were transported by Ptr2p/Ptr22p peptide transporters (PTR) and FMDP-containing...
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Light-Induced Transformation of the Aromatic Heptaene Antifungal Antibiotic Candicidin D into Its All-Trans Isomer
PublikacjaIllumination of the aromatic heptaene macrolide antifungal antibiotic candicicin D with UV light results in an isomerization of the molecule. The product formed after irradiation of the candicidin complex with UV light (λ=365nm), namely, iso-candicidin D, was isolated and subjected to 2D NMR studies, consisting of DQF-COSY, ROESY, TOCSY, HSQC, and HMBC experiments. The obtained spectral data unambiguously evidenced that iso-candicidin...
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The complete stereochemistry of the antibiotic candicidin A3 (syn. ascosin A3, levorin A3)
PublikacjaHerein, the stereostructure of the aromatic heptaene macrolide (AHM) antifungal antibiotic candicidin A3 (syn. ascosin A3, levorin A3) has been established upon the 2D NMR studies, consisting of DQF-COSY, TOCSY, ROESY, HSQC and HMBC experiments, as well as upon extensive molecular dynamics simulations. The geometry of the heptaenic chromophore was defined as: (22E, 24E, 26Z, 28Z, 30E, 32E, 34E). The previously unreported absolute...
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Accurate rolling resistance Rolling resistance measurements can be influenced by a number of factors
PublikacjaTire rolling resistance is one of the most difficult-to-measure parameters of the tire/ pavement interface, especially if road measurements are required. First of all, it is necessary to measure a fairly small force in the system that is heavily loaded by other forces and subjected to many adverse effects causing measurement errors. The second problem is related to the fact that tire rolling resistance depends on many factors,...
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A role for accessory genes rI.-1 and rI.1 in the regulation of lysis inhibition by bacteriophage T4
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Correlation between the number of Pro-Ala repeats in the EmrA homologue of Acinetobacter baumannii and resistance to netilmicin, tobramycin, imipenem and ceftazidime
PublikacjaAcinetobacter baumannii coccobacilli are dangerous to patients in intensive care units because of their multidrug resistance to antibiotics, developed mainly in the past decade. This study aimed to examine whether there is a significant correlation between the number of Pro-Ala repeats in the CAP01997 protein, the EmrA homologue of A. baumannii, and resistance to antibiotics. A total of 79 multidrug-resistant A. baumannii strains...
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Greenness assessment of analytical methods used for antibiotic residues determination in food samples
PublikacjaFood of animal origin plays an important role in ensuring a healthy and sustainable human diet. However, residues of pharmaceuticals, especially antibiotics, can be the cause of many health problems. To protect consumers and improve food quality, analytical methods used to determine contaminants should be simple, selective, and have high throughput and low detection limits. Recently, greenness metric tools have proven useful in...
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A five-year retrospective study shows increasing rates of antimicrobial drug resistance in Cabo Verde for both Staphylococcus aureus and Escherichia coli
PublikacjaObjectives Data on baseline drug resistance is important in informing future antimicrobial stewardship programs. So far, no data on the antimicrobial drug resistance of clinical isolates was available for the African archipelago of Cabo Verde. Methods We have performed a retrospective analysis over five-years (2013-17) of the antimicrombial drug susceptibility profiles of clinical isolates in the two main hospitals of Cabo Verde....
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Antibiotic usage at a clinical paediatric hospital before and after the implementation of actions related to the hospital antibiotic policy
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Final report on noise and rolling resistance
PublikacjaWork Package 6 deals with environmental impacts of PERS use, including noise and rolling resistance. This report covers preliminary noise tests performed in the laboratory and at the road test sites. The obtained results indicate that PERS material reduce noise considerably - up to 12 dB in comparison to SMA16 reference surface. Noise reduction properties are especially visible in the case of factory produced PERS slabs supplied...
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Methods of Tire Rolling Resistance Measurements
PublikacjaTire rolling resistance is one of the factors related to tire/road interaction that has important influence on road vehicles performance. Raising fuel price and increased concern related to the environmental changes result in growing interest in reduction of tire rolling resistance that influences fuel consumption, especially in free-flowing highway traffic with moderate speeds. There are several methods of tire rolling resistance measurements....
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The Effect of Posaconazole, Itraconazole and Voriconazole in the Culture Medium on Aspergillus fumigatus Triazole Resistance
PublikacjaTriazoles are the only compounds used as antibiotics in both medicine and agriculture. The presence of triazoles in the environment can contribute to the acquisition of azole resistance among isolates of Aspergillus fumigatus. The objective of this study was to investigate the effect of A. fumigatus exposure to triazoles on susceptibility to these compounds. Seventeen triazole-resistant and 21 triazole-sensitive A. fumigatus isolates...
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Road texture influence on tyre rolling resistance
PublikacjaTyre performance, one of the critical factors for vehicle users, is strongly related to theroad surface characteristics, most notably to the pavement texture. Phenomena that occur at the tyre/road interface affect tyre friction (skid resistance), rolling resistance, tyre wear and tyre/road noise. This article deals with relationship between surface texture and rolling resistance of light and heavy vehicle tyres. Mean profile depth...
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Influence of Escherichia coli on Expression of Selected Human Drug Addiction Genes
PublikacjaThe impact of enteric microflora on the expression of genes associated with cocaine and amphetamine addiction was described. Human genome-wide experiments on RNA transcripts expressed in response to three selected Escherichia coli strains allowed for significant alteration (p > 0.05) of the linear regression model between HT-29 RNA transcripts associated with the KEGG pathway:hsa05030:Cocaine addiction after 3 h stimulation with...
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Diversity of fecal coliforms and their antimicrobial resistance patterns in wastewater treatment model plant.
PublikacjaThe occurrence of resistance patterns among wastewater fecal coliforms was determined in the study. Susceptibility of the isolates was tested against 19 antimicrobial agents: aminoglycosides, aztreonam, carbapenems, cephalosporines, b-lactam/b-lactamase inhibitors, penicillines, tetracycline, trimethoprim/sulfamethoxazole, and fluoroquinolones. Additionally the removal of resistant isolates was evaluated in the laboratory-scale...
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Ship Resistance Prediction with Artificial Neural Networks
PublikacjaThe paper is dedicated to a new method of ship’s resistance prediction using Artificial Neural Network (ANN). In the initial stage selected ships parameters are prepared to be used as a training and validation sets. Next step is to verify several network structures and to determine parameters with the highest influence on the result resistance. Finally, other parameters expected to impact the resistance are proposed. The research utilizes...
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Impact of probability distribution on the uncertainty of resistance measurement
PublikacjaThe paper presents studies on the influence of probability distributions on the expanded uncertainty of the resistance measurement. Choosing the correct probability distribution is very important to estimate of measurement uncertainty. The paper presents the results of analysis of the resistance measurement uncertainty using the technical method of resistance: 100 G. The analysis of the uncertainty...
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Biofilm Formation and Prevalence of Biofilm-Related Genes Among Clinical Strains of Multidrug-Resistant Staphylococcus aureus
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INFLUENCE OF LOAD AND INFLATION PRESSURE ON THE TYRE ROLLING RESISTANCE
PublikacjaTyre load and inflation pressure are important factors controlling rolling resistance of road vehicles. The article presents results obtained in the Technical University of Gdańsk during laboratory and road measurements of different car tyres rolling on different pavements. The knowledge of rolling resistance characteristics is important for modelling car dynamics as well as fuel consumption. It is also necessary to establish proper...
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INFLUENCE OF TEMPERATURE ON THE TYRE ROLLING RESISTANCE
PublikacjaTemperature is a very important factor controlling rolling resistance of road vehicle tyres. There are at least three different temperatures that may be considered as important factors controlling thermal conditions of the rolling tyre. The most common measure of the thermal conditions during tyre rolling is ambient air temperature. The other two are: pavement temperature and “tyre” temperature. Tyre temperature is the most difficult...
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Engineering method of tire rolling resistance evaluation
PublikacjaTire rolling resistance is one of the most difficult tire parameters to measure. The reason is that for modern tires the force of rolling resistance corresponds to 0.5–1% of tire load, thus measurements of very small forces must be performed in a heavily loaded system. This constitutes great problems, especially in road conditions. Laboratory measurements are easier to perform, as the environment may be better controlled, but roadwheel...
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Antibacterial Activity and Cytocompatibility of Bone Cement Enriched with Antibiotic, Nanosilver, and Nanocopper for Bone Regeneration
PublikacjaBacterial infections due to bone replacement surgeries require modifications of bone cement with antibacterial components. This study aimed to investigate whether the incorporation of gentamicin or nanometals into bone cement may reduce and to what extent bacterial growth without the loss of overall cytocompatibility and adverse effects in vitro. The bone cement Cemex was used as the base material, modified either with gentamicin...
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Road texture influence on tire rolling resistance
PublikacjaTire rolling resistance is one of the most important resistive forces acting on road vehicles. It depends on tire and surface characteristics as well as vehicle operating parameters. This paper deals with the influence of road surface texture on rolling resistance of passenger car tires. Results of road measurements performed on variety of road surfaces are presented.
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Tyre rolling resistance and its influence on fuel consumption
PublikacjaRolling resistance of tyres is one of the major resistive forces acting on any wheeled vehicle. Unfortunately, it is also one of the forces very difficult to measure. It is estimated that in certain traffic conditions (like for example constant speed driving with slow or moderate speed) so called Rolling Resistance Impact Factor may be as high as 0.3. This means that reduction of rolling resistance by 50% would lead to 15% of energy...
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Epigenetic regulation and regeneration: the search for differentially methylated genes in the MRL mouse
PublikacjaThe MRL mouse is an inbred laboratory strain, which was developed in the 60’s of the 20th century and has been extensively used as a model of lupus erythematosus. The regenerative abilities of the strain were discovered in the 90' when the MRL mouse was shown to close 2 mm hole punches made in the ear pinnae four weeks after injury without scarring. The phenomenon has not been observed in other mouse strains, where the holes...
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Decreasing CO2 Emissions By Reducing Tire Rolling Resistance
PublikacjaThe first motor vehicle was built by Nicolas Cugnot almost 250 years ago and since then there has been a continuous process of motor vehicles’ improvements, as well as development of new road pavements. The fuel crisis of the 70s of the 20th century made it clear to vehicle builders that measures should be taken to reduce vehicle fuel consumption and it contributed to an increased interest in electric vehicles. Although significant...
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Influence of Road Wetness on Tire-Pavement Rolling Resistance
PublikacjaRolling resistance of tires is one of the most important factors influencing energy consumption of road vehicles, especially on rural roads. For practical reasons, most of rolling resistance measurements are usually performed for dry road conditions. Based on the fact that roads are wet during a considerable time over the year and as part of the projects MIRIAM, ROLRES and ROSANNE, the TUG (Technical University of Gdańsk) in Poland...
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Starter cultures as a reservoir of antibiotic resistant microorganisms
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Absolute configurations of C34 and C35 of antibiotic niphimycin A
PublikacjaThe relative configurations of four stereogenic centers of the C33-C42 fragment of niphimycin A were assigned as 2S*, 3R*, 4S* and 6S*, based upon (1)H NMR analysis with double-quantum filtered COSY and nuclear Overhauser spectroscopy experiments. These data were then correlated with absolute configurations at C36 and C38 of niphimycin A, which were declared previously as 36S and 38S [3]. This allowed for the assignment of the...
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Absolute configurations of C34 and C35 of antibiotic niphimycin A
PublikacjaThe relative configurations of four stereogenic centers of the C33-C42 fragment of niphimycin A were assigned as 2S*, 3R*, 4S* and 6S*, based upon 1H NMR analysis with double-quantum filtered COSY and nuclear Overhauser spectroscopy experiments. These data were then correlated with absolute configurations at C36 and C38 of niphimycin A, which were declared previously as 36S and 38S [3]. This allowed for the assignment of the absolute...
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TEMPERATURE INFLUENCE ON TIRE ROLLING RESISTANCE MEASUREMENTS QUALITY
PublikacjaGlobal warming makes it necessary to reduce energy consumption, which in the case of motor vehicles, is connected, among other things, with reduction of resistive forces acting on a vehicle during its motion. One of the most important components of those forces is rolling resistance, which is very difficult to measure, especially in road conditions. The article deals with issues related to the influence of the thermal state of...
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Intramolecular transformation of an antifungal antibiotic nystatin A1 into its isomer, iso-nystatin A1 - structural and molecular modeling studies
PublikacjaNystatin A1, a polyene macrolide antifungal antibiotic, in a slightly basic or acidic solution undergoes an intramolecular transformation, yielding a structural isomer, the translactonization product, iso-nystatin A1 with lactone ring diminished by two carbon atoms. Structural evidence is provided by advanced NMR and Mass Spectrometry (MS) studies. Molecular dynamics simulations and quantum mechanics calculations gave the insight...
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Elctrochemical oxidation and corrosion resistance of the Ti13Nb13Zr alloy
PublikacjaThis paper presents the results of oxidation and corrosion tests carried out on titanium alloy Ti13Nb13Zr. The oxide film was prepared by electrochemical environment 2M H3PO4 for 30 min and 1 h, at a constant voltage 40V. The tests of corrosion resistance were performed by potentiostatic method in Ringer's solution at different pH values: 7, 5 and 3. The change in an appearance of surface and the increase in corrosion resistance...
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Analysis of Rolling Resistance of Tires With Run Flat Insert
PublikacjaRun flat properties are required for tires intended for special applications like military or rescue vehicles. Run-Flat inserts make possible to drive a car or truck, with certain restrictions, also when inflation pressure is very low or non-existing (after tire damage). Rolling resistance of tires rolling the inserts increases considerably in relation to the rolling resistance of properly inflated tires. This paper presents results...
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Analytical studies on ascosin, candicidin and levorin multicomponent antifungal antibiotic complexes. The stereostructure of ascosin A2
PublikacjaIn the class of polyene macrolides, there is a subgroup of aromatic heptaenes, which exhibit the highest antifungal activity within this type of antibiotics. Yet, due to their complex nature, aromatic heptaenes were not extensively studied and their potential as drugs is currently underexploited. Moreover, there are many inconsistencies in the literature regarding the composition and the structures of the individual components...
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Modernization of the Trailer for Tyre/Road Rolling Resistance Measurements
PublikacjaIn the article the ways of defining tyre rolling resistance are presented. The advantages of the laboratory tests of tyre/road rolling resistance and the advantages and disadvantages of on the road tyre/road rolling resistance tests are described. The description of the special trailer used for tyre/road rolling resistance measurements designed and constructed in the Faculty of Mechanical Engineering at Gdansk University of Technology,...
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Comparison of Tire Rolling Resistance Measuring Methods for Different Surfaces
PublikacjaThe rolling resistance of car tires is one of the most important parameters characterizing tires today. This resistance has a very significant contribution to the energy consumption of wheeled vehicles. The climate crisis has forced tire and car manufacturers to place great emphasis on the environmental impact of their products. Paradoxically, the development of electric vehicles has led to an even greater importance of rolling...
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Cavitation Erosion Resistance Influence of Material Properties
PublikacjaThe cavitation erosion is the phenomena that causes degradation of fluid flow machinery components due to repetitive implosion of cavitation bubbles adjacent to the solid surface. Cavitation erosion is a complex phenomenon, which includes not only hydrodynamic factors of liquid, but also properties of erodible material e.g. microstructure, hardness or Young modulus. In order to reduce the negative impact of erosion on machine components,...
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Shear resistance of low height precast concrete lintels
PublikacjaThe scope of the paper is to investigate analytically and determine experimentally the shear resistance of low height reinforced precast concrete lintels. The chosen procedures included in national and international standards applied for the design of structural concrete elements to an estimation of shear behaviour of reinforced concrete elements are described. The characteristic and designed shear strength of precast concrete...
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Self-Association of Amphotericin B: Spontaneous Formation of Molecular Structures Responsible for the Toxic Side Effects of the Antibiotic
PublikacjaAmphotericin B (AmB) is a lifesaving antibiotic used to treat deep-seated mycotic infections. Both the pharmaceutical activity and highly toxic side effects of the drug rely on its interaction with biomembranes, which is governed by the molecular organization of AmB. In the present work we present detailed analysis of self-assembly of AmB molecules in different environments, interesting from the physiological standpoint, based...
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The influence of road surface unevenness on tyre rolling resistance
PublikacjaThe geometric characteristics of road surface substantially affect the interaction between tyre and road. Depending on pavement texture wavelength, the texture chiefly affects tyre/road friction, rolling resistance, interior and exterior noise, tyre wear, and ride comfort. The article presents results of investigations on the influence of road surface unevenness on the rolling resistance of passenger car and truck tyres. The tests...
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Antimicrobial resistance of fecal indicators in disinfected wastewater
PublikacjaThe main objective of the study was to assess the potential of three systems (UV irradiation, ozonation, and micro/ultrafiltration) operated in a pilot scale in removal of antimicrobial-resistant fecal bacteria from secondary effluent of the local wastewater treatment plant (700,000 population equivalent). The effectiveness of the processes was analysed using the removal ratio of fecal indicators (Escherichia coli and Enterococcus...
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Antibiotic-sterol interactions provide insight into the selectivity of natural aromatic analogues of amphotericin B and their photoisomers
PublikacjaAromatic heptaene macrolides (AHMs) belong to the group of polyene macrolide antifungal antibiotics. Members of this group were the first to be used in the treatment of systemic fungal infections. Amphotericin B (AmB), a non-aromatic representative of heptaene macrolides, is of significant clinical importance in the treatment of internal mycoses. It includes the all-trans heptaene chromophore, whereas the native AHMs contain two...
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Selected Aspects of Pavement Texture Influence on Tire Rolling Resistance
PublikacjaTire rolling resistance has a significant influence on fuel consumption in cars and trucks and on CO2 emissions. Rolling resistance depends on the tire construction, pavement texture and stiffness, as well as environmental and traffic conditions. This article presents a pilot study on the impact of pavement texture on the rolling resistance of passenger car tires. Reasons why Mean Profile Depth is not a good descriptor of pavement...
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Fecal indicators resistance to antimicrobial agents present in municipal wastewater
PublikacjaIn this study removal rate of six antimicrobial compounds (belonging to folate pathway inhibitors and macrolides class) was analyzed in processes of local wastewater treatment plant (WWTP) together with susceptibility patterns of erythromycin (E) resistant Enterococcus spp. and trimethoprim/sulfamethoxazole (SXT) resistant Escherichia coli. According to the obtained data, six of tested antimicrobial agents, namely erythromycin,...
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The resistance of S235JR steel to cavitation erosion
PublikacjaThe erosion resistance of S235JR steel to flow cavitation was investigated. In order to understand an influence of steel properties and cavitation intensities on the degradation mechanisms, low-carbon S235JR steel was tested in the as-received state and after thermal treatment (annealing) under four different flow velocities. Annealing decreased steel hardness, which made S235JR steel less resistant to cavitation erosion. The performed...
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Microbial response of the anammox process to trace antibiotic concentration
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