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Principles of target DNA cleavage and the role of Mg2+ in the catalysis of CRISPR–Cas9
PublikacjaAt the core of the CRISPR–Cas9 genome-editing technology, the endonuclease Cas9 introduces site-specific breaks in DNA. However, precise mechanistic information to ameliorate Cas9 function is still missing. Here, multimicrosecond molecular dynamics, free energy and multiscale simulations are combined with solution NMR and DNA cleavage experiments to resolve the catalytic mechanism of target DNA cleavage. We show that the conformation...
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Modeling of the Two-Dimensional Flow Caused by Sea Conditions and Wind Stresses on the Example of Dead Vistula
PublikacjaThe article presents the results of two-dimensional modeling of flows caused by the sea conditions and wind stresses on the example of Dead Vistula. Based on the available bathymetric data, a numerical model of the river section was created, which was supplemented with data on the position of the water table depending on hydrometeorological conditions. To describe the flow field in steady conditions, a simplified model of two-dimensional...
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Reprezentation of mechanic hysteresis in a railway track using the Preisach model
PublikacjaThis paper presents an implementation of the classic scalar Preisach model to describe the hysteresis phenomenon created in a railway track subjected to lateral (in the horizontal plane) displacements. The principles of the Preisach theory and the scalar mathematical model of the mechanical hysteresis that is based on statistically distributed modified hysteresis operators are briefly presented. This article presents the characteristic...
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Time Delay Estimation in Two-Phase Flow Investigation Using the γ-Ray Attenuation Technique.
PublikacjaTime delay estimation is an important research question having many applications in a range of technologies. Measurement of a two-phase flow in a pipeline or an open channel using radioisotopes is an example of such application. For instance, the determination of velocity of dispersed phase in that case is based on estimation of the time delay between two stochastic signals provided by scintillation probes. The proper analysis...
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Impact Behaviour of Glass Fribre /Epoxy Composites with Nano-Enhanced Resin after Water Exposure
PublikacjaImpact behaviour of glass fibre /epoxy composites with nano- SiO2 modified resin was studied in terms of low velocity impact after water exposure. Nanocomposites with 1%, 2%, 3% 5% 7% nano-SiO2 (Nanopox- Evonic) were investigated. Peak impact load and impact damage area as a function of nanoparticle contents were compared for dry specimens and for samples exposed to water (0.7 %wt. 1.7% water absorbed) at 1J, 2J 3J impact energies....
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Effects of Bromine Doping on the Structural Properties and Band Gap of CH3NH3Pb(I1–xBrx)3 Perovskite
PublikacjaAn experimental and theoretical study is reported to investigate the influence of bromine doping on CH3NH3Pb(I1−xBrx)3 perovskite for Br compositions ranging from x = 0 to x = 0.1, in which the material remains in the tetragonal phase. The experimental band gap is deduced from UV−vis absorption spectroscopy and displays a linear behavior as a function of bromine concentration. Density functional theory calculations are performed...
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Experimental Investigations of Fracture Process Using DIC in Plain and Reinforced Concrete Beams under Bending
PublikacjaThe fracture behaviour of concrete and reinforced concrete beams under quasi-static three-point bending was comprehensively investigated with experiments at laboratory scale. The eight various concrete mixes were tested. The influence of the shape, volume and size of aggregate particles and reinforcement on concrete fracture under bending was studied. Displacements on the surface of concrete beams were measured by means of the...
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Programmable dynamically changing RC model for evaluation of Dynamic EIS methods and instrumentation
PublikacjaThe paper concerns the RC equivalent circuit of electrochemical cell with the impedance changed during the experiment. The model was constructed using the digital potentiometers controlled by the microcontroller. This solution allows to control the model impedance by the software means. By creating adequate changes of the impedance spectrum as a time function, the different impedance measurement method can be tested to find out...
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Application of a catalytic combustion sensor (Pellistor) for the monitoring of the explosiveness of a hydrogen-air mixture in the upper explosive limit range
PublikacjaA new technique is presented for continuous measurements of hydrogen contamination by air in the upper explosive limit range. It is based on the application of a catalytic combustion sensor placed in a cell through which the tested sample passes. The air content is the function of the quantity of formed heat during catalytic combustion of hydrogen inside the sensor. There is the possibility of using the method in industrial installations...
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Calculation of self and mutual inductances of the switched reluctance machine mathematical model.
PublikacjaA mathematical model of the switched reluctance machine (SRM) in a drive system obtained using Lagrange's energy method and a method of calculation of self and mutual inductances of the SRM are presented in the paper. The self and mutual inductances are elements of Lagrange's function in generalised coordinates and have been calculated using the finite element method (FEM). Selected calculation results for the particular machine...
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The evaluation of the vibration measurement usability of electronic indicator lemag "premet C"
PublikacjaThe measuring possibilities of modern compression and combustion pressure analyzers are extended with additional functions. One of them is parallel to the pressure measurement, the measurement of vibrations in the region of the cylinder head. The paper presents a general assessment of the vibration measurement function of the electronic indicator LEMAG "PREMET C". This feature is very rarely offered by manufacturers of these devices....
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Regularity of weak solutions for aclass of elliptic PDEs in Orlicz-Sobolev spaces
PublikacjaWe consider the elliptic partial differential equation in the divergence form $$-\div(\nabla G(\nabla u(x))) t + F_u (x, u(x)) = 0,$$ where $G$ is a convex, anisotropic function satisfying certain growth and ellipticity conditions We prove that weak solutions in $W^{1,G}$ are in fact of class $W^{2,2}_{loc}\cap W^{1,\infty}_{loc}$.
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Mobilność niemobilnego księgozbioru – przypadek Pomorskiej Biblioteki Cyfrowej
PublikacjaCelem artykułu jest zapoznanie czytelnika z zagadnieniem bibliotek cyfrowych oraz przybliżenie struktury i działalności Pomorskiej Biblioteki Cyfrowej [dalej: PBC]. W drugiej części artykułu ukazano rolę PBC w udostępnianiu cennych kolekcji bibliotecznych – w oparciu o dane statystyczne, ankietę przeprowadzoną wśród użytkowników PBC oraz zapytania kierowane poprzez formularz kontaktowy. Artykuł przedstawia także działania promujące...
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Adaptive dynamic control allocation for over-actuated dynamic positioning system based on backstepping method in case of thruster faults
PublikacjaThe objective of the research considered in this paper is dynamic positioning of a nonlinear over-actuated marine vessel in the presence of limited information about thruster forces. First, the adaptive backstepping method is used to estimate the input matrix which will compensate partial loss of actuator effectiveness in the presence of actuator dynamics. Then, the adaptive commanded virtual forces and moment are allocated into...
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Approximate Criteria for the Evaluation of Truly Multi-Dimensional Optimization Problems
PublikacjaIn this paper we propose new improved approximate quality criteria useful in assessing the efficiency of evolutionary multi-objective optimization (EMO). In the performed comparative study we take into account the various EMO algorithms of the state-of-the-art, in order to objectively assess the EMO performance in highly dimensional spaces. It is well known that useful executive criteria, such as those based on the true Pareto...
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Postprodukcja nagrania wideo z dzwiekiem dookolnym
PublikacjaOne of the aims of this paper is to present issues related to audio-video correlation. This is presented on the basis of a short film realization employing surround microphone techniques. First, some related works in the domain of sound and vision correlation are presented. Then assumptions concerning scene creation related to both audio and video are shortly described. Another objective is to discuss results of subjective tests...
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Speciation of heavy metals in wastewater and rejected waters from multistage bioreactors (MUCT)
PublikacjaThe objective of the study is to investigate the occurrence and availability of selected heavy metals after subsequent stages of treatment in WWTP with activated sludge (enhanced biological N and P removal). To achieved the target it was necessary to recognize the allocation of selected heavy metals between aqueous and solid phase in wastewater and reject waters. Application of sequential extraction of such metals as Cd Zn, Pb,...
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Active Dynamic Thermography imaging of wound healing processes in cardio surgery
PublikacjaThe surgery is a branch of medicine, that is integrally connected to wounds. Despite using sterile tools and compliance with aseptic rolls, some of the surgery wounds become infected. In clinical practice there is a lack of cheap, objective methods and tools for quantitative definition and estimation of the surgery wound healing progress. This paper presents preliminary results of Active Dynamic Thermography (ADT) parametric imaging...
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Observation value analysis - integral part of Bayesian diagnostics
PublikacjaDetailed subject of the research is to analyse the value of the observation, which is a part of preposterior analysis. For the presented network, the main objective was to determine, conducting of which of three tests is the most valuable from the perspective of determining possible need or possibility to omission expensive technical expertise. The main advantage of preposterior analysis is answering the question which of the considered...
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Determination of thiocyanate (biomarkers of ETS) and other inorganic ions in human nasal discharge samples using ion chromatography
PublikacjaEnvironmental tobacco smoke (ETS) is a mixture of air and tobacco smoke containing more than 4000 chemical substances. In view of the health risks of many of these substances, studies are needed to determine biomarkers of exposure to ETS constituents in people who actively or passively are exposed to the toxic compounds. The methodologies for determining most biomarkers from saliva, urine and blood samples are known, but methods...
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Restoration and preservation of the reinforced concrete poles of fence at the former Auschwitz concentration and extermination camp
PublikacjaThe objective of this study was to assess the present state of the reinforced concrete poles of fence at the former Auschwitz I and Auschwitz II-Birkenau concentration and extermination camp. The poles were subjected to renovation about 10 years ago. After this time some deficiencies of applied renovation method were noticed. Cracks appeared between fresh and original part of concrete cover. Analysis of the reasons of these failures...
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Issues of Measuring the Course of Batch Production Processes
PublikacjaIn order to meet demands induced by development of manufacturing processes and production systems, new criteria and indicators that would allow a multiple aspect and realistic rating of batch production process courses are necessary. The objective of this paper is to present the correlation between production processes measurement, its rating and production control, basing on case study analysis from production enterprise. A new...
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Restoration and preservation of the reinforced concrete poles of fence at the former Auschwitz concentration and extermination camp
PublikacjaThe objective of this study was to assess the present state of the reinforced concrete poles of fence at the former Auschwitz I and Auschwitz II-Birkenau concentration and extermination camp. The poles were subjected to renovation about 10 years ago. After this time some deficiencies of applied renovation method were noticed. Cracks appeared between fresh and original part of concrete cover. Analysis of the reasons of these failures...
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FE analysis of support-specimen interaction of compressive experimental test
PublikacjaThe objective of this work is to investigate the support-specimen interaction during the compressive experimental testing of stiffened plates. The interaction is analyzed employing the nonlinear Finite Element Method using the commercial software ANSYS. The connection between the stiffened plate and testing supports is modelled with the use of contact elements, where several possible interaction scenarios are investigated, and...
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Pin-on-Substrate Gap Waveguide: An Extremely Low-Cost Realization of High-Performance Gap Waveguide Components
PublikacjaConsidering the limitations of currently available technologies for the realization of microwave components and antennas, a trade-off between different factors including the efficiency and fabrication cost is required. The main objective of this letter is to propose a novel method for the realization of gap waveguides (GWGs) that take advantage of conventional PCB fabrication technology, thus are low cost and light weight. Moreover,...
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Measurements of Two-phase Flows in Pipelines Using Radioisotopes and Statistical Signal Processing
PublikacjaThis paper presents an application of radiotracers and gamma absorption method in two-phase flow measurements in pipelines. Two different methods were implemented to analysis of acquired signals. Investigated methods are based on the cross-correlation function and the phase of the cross-spectral density distribution. The examples presented in the article illustrate the application of the radioisotopes to evaluation of liquid-gas...
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Bezpieczeństwo funkcjonalne: określanie wymaganego poziomu nienaruszalności bezpieczeństwa SIL - cz. 2
PublikacjaBezpieczeństwo funkcjonalne pełni jedną z kluczowych ról w procesie zarządzania bezpieczeństwem w cyklu życia systemu technicznego i dotyczy właściwego projektowania oraz późniejszego utrzymywania systemów technicznych związanych z bezpieczeństwem, wykonanych w technologii E/E/PE (elementy elektryczne / elektroniczne / elektroniczne programowalne) realizujących tzw. funkcje bezpieczeństwa SRF (ang. safety related function).
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Estimation of the uncertainty of the AC/AC transducer output voltage
PublikacjaThe paper presents a methodology for estimating uncertainty of voltage transducers processing function, on the example of CYVS411D07 voltage transducers. The analysis was conducted using the method based on the GUM Guide propagation of uncertainty law and the determination of the coverage factor based on the effective number of degrees of freedom (t-Student distribution). The paper also presents example evaluation results of measured...
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How to apply the probabilistic model of measurement processes successfully
PublikacjaApplicational aspects of probabilistic model of measurement processes, proposed by G.B. Rossi, are considered. The main idea of the model - using of Bayes-Laplace postulate for solution of inverse probability problem, is substituted by Fisher's concept of the likelihood function, expressed in the data translated format. This approach gives a clear-sighted solution of inverse probability. Some recommendations for application of...
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Fully Adaptive Savitzky-Golay Type Smoothers
PublikacjaThe problem of adaptive signal smoothing is consid-ered and solved using the weighted basis function approach. Inthe special case of polynomial basis and uniform weighting theproposed method reduces down to the celebrated Savitzky-Golaysmoother. Data adaptiveness is achieved via parallel estimation.It is shown that for the polynomial and harmonic bases andcosinusoidal weighting sequences, the competing signal estimatescan be computed...
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On the Bending of Multilayered Plates Considering Surface Viscoelasticity
PublikacjaWe discuss the bending resistance of multilayered plates taking into account surface/interfacial viscoelasticity. Within the linear surface viscoelasticity we introduce the surface/interfacial stresses linearly dependent on the history of surface strains. In order to underline the surface viscoelasticity contribution to the bending response we restrict ourselves to the elastic behaviour in the bulk. Using the correspondence principle...
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Żywność funkcjonalna- najważniejsze osiągnięcia w zakresie badań i wykorzystania
PublikacjaW wielu ośrodkach naukowych trwają badania ukierunkowane na poznanie mechanizmów działania składników żywności funkcjonalnej na poziomie ekspresji genetycznej, co stwarza możliwość precyzyjniejszego scharakteryzowania funkcji bioaktywnych składników diety z uwzględnieniem zmienności genetycznej populacji. Daje to w efekcie bardziej wiarygodne wyniki badań naukowych dotyczących wpływu bioaktywnych składników diety na funkcjonowanie...
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Using Minimum Actuators to Control Shape and Stress of a Double Layer Spherical Model Under Gravity and Lateral Loadings
PublikacjaSpherical domes are picturesque structures built in developed countries to attract tourists. Due to horizontal and vertical overloading, the structures’ attractive shapes may be disturbed, and some members' stress may exceed the elastic level. In this paper, the shape and stress of a deformed double-layer spherical numerical model due to simultaneous lateral and vertical loadings are controlled, meanwhile, the number of actuators...
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Impact of Temperature and Nanoparticle Concentration on Turbulent Forced Convective Heat Transfer of Nanofluids
PublikacjaTheoretical analysis of the influence of nanoparticles and temperature on the average Nusselt (Nu) number and the average heat transfer coefficient (HTC) during the turbulent flow of nanofluid in a horizontal, round tube was carried out. The Nu number is a function of the Reynolds (Re) number and the Prandtl (Pr) number, which in turn are functions of the thermophysical properties of the liquid and the flow conditions. On the other...
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Pressure drop related to flow maldistribution in a model minichannel plate heat exchanger
PublikacjaThe paper describes the issues related to the pressure drop that accompanies the phenomenon of maldistribution of working fluid between the channels of a model minichannel plate heat exchanger. The research concerns a single exchanger's plate containing 51 (in every geometry) parallel rectangular minichannels of hydraulic diameters 461 µm, 571 µm, 750 µm and 823 µm. In addition, the more complex geometry has been investigated,...
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Controlled grafting of vinylic monomers on polyolefins: a robust mathematical modeling approach
PublikacjaExperimental and mathematical modeling analyses were used for controlling melt free-radical grafting of vinylic monomers on polyolefins and, thereby, reducing the disturbance of undesired cross-linking of polyolefins. Response surface, desirability function, and artificial intelligence methodologies were blended to modeling/optimization of grafting reaction in terms of vinylic monomer content, peroxide initiator concentration,...
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The impedance of hydrogen oxidation reaction in a proton exchange membrane fuel cell in the presence of carbon monoxide in hydrogen stream
PublikacjaEvaluation of performance of a proton exchange membrane fuel cell, which is affected by carbon monoxide that pollutes the hydrogen stream, was presented. This influence was studied for carbon monoxide concentration of 125–325 ppb, which are close to values specified in ISO 14687:2019 standard. Performed studies provided crucial information for further development of fuel cell as an energy source for automotive application. Impedance...
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Self-Adaptive Mesh Generator for Global Complex Roots and Poles Finding Algorithm
PublikacjaIn any global method of searching for roots and poles, increasing the number of samples increases the chances of finding them precisely in a given area. However, the global complex roots and poles finding algorithm (GRPF) (as one of the few) has direct control over the accuracy of the results. In addition, this algorithm has a simple condition for finding all roots and poles in a given area: it only requires a sufficiently dense...
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Constant-Factor Approximation Algorithm for Binary Search in Trees with Monotonic Query Times
PublikacjaWe consider a generalization of binary search in linear orders to the domain of weighted trees. The goal is to design an adaptive search strategy whose aim is to locate an unknown target vertex of a given tree. Each query to a vertex v incurs a non-negative cost ω(v) (that can be interpreted as the duration of the query) and returns a feedback that either v is the target or the edge incident to v is given that is on the path towards...
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A Diagnostic Method Based on Deflection Analysis for Monitoring Post-elastic Response of Beams
PublikacjaA non-destructive diagnostic method based on deflection analysis for monitoring the post-elastic response of beams was developed, and a diagnostic indicator was proposed. The indicator was defined as the second moment of the normalised curvature function about the mid-span, where the curvature was computed from the normalised deflection function. Elastic reference values of the indicator were computed for simply supported beams...
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Generalized Einstein relation in disordered organic semiconductors: Influence of the acoustic phonons–charge carriers scattering
PublikacjaIn this work, we analyze the generalized Einstein relation for disordered organic semiconductors with a non-equilibrium Druyvesteyn-type distribution function. The Druyvesteyn behavior of hot electrons in a solid state is associated with the acoustic phonons–charge carriers scattering. Such a case has been experimentally demonstrated in electroluminescent inorganic rare–earth–doped zinc chalcogenides. Therefore, we can assume that,...
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Using Long-Short term Memory networks with Genetic Algorithm to predict engine condition
PublikacjaPredictive maintenance (PdM) is a type of approach for maintenance processes, allowing maintenance actions to be managed depending on the machine's current condition. Maintenance is therefore carried out before failures occur. The approach doesn’t only help avoid abrupt failures but also helps lower maintenance cost and provides possibilities to manufacturers to manage maintenance budgets in a more efficient way. A new deep neural...
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Reinforcement Learning Algorithm and FDTD-based Simulation Applied to Schroeder Diffuser Design Optimization
PublikacjaThe aim of this paper is to propose a novel approach to the algorithmic design of Schroeder acoustic diffusers employing a deep learning optimization algorithm and a fitness function based on a computer simulation of the propagation of acoustic waves. The deep learning method employed for the research is a deep policy gradient algorithm. It is used as a tool for carrying out a sequential optimization process the goal of which is...
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Tailoring optical constants of few-layer black phosphorus coatings: Spectroscopic ellipsometry approach supported by ab-initio simulation
Publikacja2D black phosphorus (BP) has attracted extensive attention as an anisotropic platform for novel optoelectronic and polarizing optics applications. Insight into the factors that tune the optical and polarizing properties of 2D BP reveals their essential influence on BP-based photonic and optoelectronic devices. In this work, studies of the optical constants of few-layer black phosphorus coatings are studied and discussed, with particular...
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Relevance of Urban Freight Transport Modelling Towards the Challenges of Urban Freight Policy
PublikacjaThe main objective of this paper is to investigate how recent trends in urban freight transport analysis and modelling are related to urban freight measures set out in Gdynia’s 2016 Sustainable Urban Mobility Plan. Building on the literature a review framework was defined including 1) stakeholders, 2) descriptors, 3) objectives and 4) solution approach applied in freight modelling efforts. It was clear that the implementation...
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Ocena zastosowania wybranych reguł wymiany narzędzi w systemie produkcyjnym
PublikacjaIn this paper, an integrated analytical and simulation modelling framework has been developed with the objective to explore total system planning and operational issues. It enables in particular structuring the alternative process flow in manufacturing systems and their extended investigation under different tool exchange rules with limited number of tool inventory. Simulation studies performed with the proposed rules in the environment...
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A fuzzy logic model for forecasting exchange rates
PublikacjaThis article is devoted to the issue of forecasting exchange rates. The objective of the conducted research is to develop a predictive model with the use of an innovative methodology - fuzzy logic theory - and to evaluate its effectiveness in times of prosperity and during the financial crisis. The model is based on sets of rules written by the author in the form of IF-THEN, where expert knowledge is stored. This model is the result...
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Optimised allocation of actuators for DWDS
PublikacjaThis paper addresses the problem of actuators’ allocation within networked structured system, namely allocation of disinfectant booster stations within Drinking Water Distribution System (DWDS), under receding horizon optimised control – Model Predictive Control (MPC) to be exact. The allocation task is kept within dynamic multiobjective optimisation framework. The MPC is defined as a single objective predictive operator. Two numerical...
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Novel 5.1 Downmix Algorithm with Improved Dialogue Intelligibility
PublikacjaA new algorithm for 5.1 to stereo downmix is introduced, which addresses the problem of dialogue intelligibility. The algorithm utilizes proposed signal processing algorithms to enhance the intelligibility of movie dialogues, especially in difficult listening conditions or in compromised speaker setup. To account for the latter, a playback configuration utilizing a portable device, i.e. an ultrabook, is examined. The experiments...
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SEMANTIC ANALYSIS ALGORITHMS FOR KNOWLEDGE WORKERS SUPPORT
PublikacjaThe paper examines various aspects of text analysis application for knowledge worker’s activity realization. Conclusions are drawn about the relevance and importance of processing the non-structured textual information in order to increase knowledge worker’s efficiency, as well as their awareness in different branches of science. The paper considers the existing algorithms of texts semantic analysis as the sphere of documents topical...