Filtry
wszystkich: 1627
wybranych: 1118
-
Katalog
- Publikacje 1118 wyników po odfiltrowaniu
- Czasopisma 31 wyników po odfiltrowaniu
- Osoby 36 wyników po odfiltrowaniu
- Wynalazki 1 wyników po odfiltrowaniu
- Projekty 6 wyników po odfiltrowaniu
- Laboratoria 1 wyników po odfiltrowaniu
- Kursy Online 30 wyników po odfiltrowaniu
- Wydarzenia 11 wyników po odfiltrowaniu
- Dane Badawcze 393 wyników po odfiltrowaniu
Filtry wybranego katalogu
wyświetlamy 1000 najlepszych wyników Pomoc
Wyniki wyszukiwania dla: EPD
-
Post‐Second World War Reconstruction of Polish Cities: The Interplay Between Politics and Paradigms
PublikacjaBy the end of the Second World War, many of the Polish cities—and especially their historic centres—were in ruins. This was caused by both bombings and sieges conducted by the Nazis and Soviets. The particular group of cities is associated with former German lands—now called the “Recovered Territories”—which were incorporated into the borders of Poland as compensation for its Eastern Borderlands lost to the Soviet Union. These...
-
Optical recognition elements. Macrocyclic imidazole chromoionophores entrapped in silica xerogel
PublikacjaNowe chromojonofory zawierające fragment eteru koronowego i resztę azolu zostały unieruchomione w krzemionkowej matrycy żelowej metodą zol-żel i zbadane jako optyczne elementy rozpoznające kationy Li+,Cs+, Cu2+. Elementy optyczne oparte na 21-członowych pochodnych imidazolu i 4-metyloimidazolu rozpoznawały jony Li+ w roztworze wodnym. Wyznaczono parametry g i A na podstawie badań EPR dla kompleksów 18-członowej i 21-członowej pochodnej...
-
Economic benefits of dynamic charging of electric buses
PublikacjaDiesel engines buses are still the most used type of buses. Electric buses provide promising green alternatives and a lot of advantages, but their main disadvantages are limited travel range and long charging time. This article is a presentation of innovative solution for charging of electric busses - Dynamic Charging (IMC). The modern IMC system in Solingen was presented. At the end of the article, a proposal was made to introduce...
-
Position in global value chains and wages in Central and Eastern European countries
PublikacjaThis paper examines the relationship between the relative position of industries in Global Value Chains (GVC) and wages in 10 Central and Eastern European countries. We combine GVC measures of global import intensity of production, upstreamness and the length of the value chain with micro-data on workers. We find that the wages of Central and Eastern European countries workers are higher when their industry is at the beginning...
-
Investigation of continuous wave jamming in an IEEE 802.15.4 network
PublikacjaThis paper presents how continuous wave jamming affects IEEE 802.15.4 network. To this end, an office-based measurement setup has been proposed. Within the measurement area, 25 nodes have been set up in order to create a IEEE 802.15.4 tree-based test network structure. A dedicated jamming device that generates and transmits a continuous wave signal has been developed. Several tests have been conducted and presented to demonstrate...
-
An Idea Bag strategy to discourage selfish behavior under random token MAC protocols for wireless LANs
PublikacjaDla klasy protokołów MAC opartych na przypadkowo krążącym żetonie dononano rozdziału polityki wyłaniania zwycięzcy od strategii wyboru szczeliny dla żądania dostępu. Wyróżniono klasę protokołów RT/ECD, dla której zidentyfikowano typ wynikowej gry niekooperacyjnej. Pokazano, że gra ta zasadniczo różni się od wieloosobowego dylematu więźnia. Zaprojektowano heurystyczną strategię asymptotycznie zniechęcającą do zachowań egoistycznych...
-
Elliptical slot UWB monopole antenna
PublikacjaThe UWB monopole antenna with elliptical radiating slot end and microstrip feeding line terminated with radial stub have been designed, fabricated and measured. The antenna show return loss less than -12 dB in the whole UWB bandwidth. Linearity of the reflection coefficient phase product have also been achieved. Radiation patterns of the antenna measured at three selected frequency points indicated regular, approximately omni directional...
-
Effect of WC on electroposition and corossion behaviour of chromium coatings
PublikacjaWłaściwości warstw chromowych zawierających różne ilości drobin WC zbadano metodą XPS, EPS oraz XRD. Metody te pozwoliły określić morfologię i skład chemiczny powłok. Właściwości antykorozyjne określono dla różnych czasów ekspozycji próbek w elektrolicie zawierającym 0,01 mol L-1 za pomocą metody woltamperometrycznej oraz spektroskopii impedancyjnej. Badania wykazały, że obecność WC w warstwach chromowych otrzymanych z kąpieli...
-
Rational Use of Primary Energy in Single-Family Residential Houses
PublikacjaThis work presents results of the primary energy use for heating residential single-family house. Analysis includes domestic hot water system (DHW) and heating, ventilation and air condition system (HVAC). During researches the conventional and alternative energy sources (natural gas, biomass, fuel oil, bituminous coal, lignite or electric energy obtained from electric power system – EPS) were used in DHW and HVAC systems. Furthermore...
-
Histaminol and Its Complexes with Copper(II) - Studies in Solid State and Solution
PublikacjaHistaminol [4(5)-(B-hydroxyethyl)imidazole, 4-(1H-imidazol-2-yl)ethanol, L] is an analogue of histamine and its minor metabolite. So far its properties have not been studied in detail due to the synthetic difficulties. Here, the structure and acid-base properties of histaminol, as well as the results of studies on its copper(II) complexes in solid state and aqueous solution are reported. Stability constants of the histaminol–CuII...
-
The forecasted values of cutting power for sawing on band sawing machines for Polish Scots pine wood (Pinus sylvestris L.) in a function of its provenance.
PublikacjaIn this paper the predicted values of cutting power for band sawing machine (EB 1800, f. EWD), which is used in the Polish sawmills, were showed. The values of cutting power were forecasted for Scots pine (Pinus sylvestris L.) wood of five provenances from Poland. These values were determined using an innovative method of predicting the cutting power, which takes into account of elements of fracture mechanics. The resulting predictions...
-
Optical Magnetometry Based on Nanodiamonds with Nitrogen-Vacancy Color Centers
PublikacjaNitrogen-vacancy color centers in diamond are a very promising medium for many sensing applications such as magnetometry and thermometry. In this work, we study nanodiamonds deposited from a suspension onto glass substrates. Fluorescence and optically detected magnetic resonance spectra recorded with the dried-out nanodiamond ensembles are presented and a suitable scheme for tracking the magnetic-field value using a continuous...
-
Physical properties of the uranium ternary compounds U3Bi4M3 (M=Ni,Rh)
PublikacjaW pracy dyskutowany jest wzrost kryształów i właściwości fizyczne dwóch, nowych, izostrukturalnych związków U3Bi4Ni3 i U3Bi4Rh3. Pierwszy z nich jest nie-metalem, w drugim obserwowany jest tzw. ferromagnetyczny kwantowy punkt krytyczny.
-
Rekonstrukcja ewolucji układu przestrzennego średniowiecznego miasta i portu Szczecin Reconstruction of the Spatial Evolution of the Medieval City and Port of Szczecin
PublikacjaPraca rekonstruuje ewolucję układu osadniczego Szczecina w okresie od połowy VIII w. do początku XIV w., w oparciu o interpretację dostępnej literatury naukowej dotyczącej historii i archeologii Szczecina oraz metodę analizy planistycznej. Bardzo istotnym elementem opisu struktury urbanistycznej wczesnośredniowiecznego Szczecina było określenie roli i położenia portu. W wyniku analizy zmian układu przestrzennego miasta w omawianym...
-
Początek Gdyńskiego Systemu Wodociągowego Rozbudowa układu wodociągowego na Kępie Oksywskiej w latach 1933 - 1939. Część III.
PublikacjaBudowa sieci osiedlowej na terenie „Nowego Oksywia”. Przyłączenie wodociągu Marynarki Wojennej do budowanego układu na Kępie Oksywskiej. Komplikacje przebiegu procesu budowy rurociągu do Babiego Dołu. Uzbrojenie terenu osiedla "Paged". Przekształcenie na terenie Oksywia struktury sieci rozgałęzieniowej w pierścieniową. Konsolidacja układu na Kępie Oksywskiej z wodociągiem grupowym Wielkiej Gdyni. Realizacja zarządzenia o przymusie...
-
[Chapter] 22. Application of physical modeling to study combustion process-es and flow patterns in large-scale boilers and furmaces. W: Optical me- thods and data processing in heat and fluid flow. Ed. C. Greated, J. Cos-grove, J.M. Buick. Bury St. Edmunds. London: Profess. Eng. Publ.**2002 s. 267-277, 4 rys. bibliogr. 6 poz. Zastosowanie modelowania fizycznego do badania procesów spalania pola prze- pływu w przemysłowych kotłach i piecach.
PublikacjaRozdział zawiera wyniki badań modelowania fizycznego dwuwymiarowego i trój-wymiarowego pola przepływu i procesów spalania w wybranych urządzeniachprzemysłowych.
-
Wielkomiejskie budowle mostowe dla ruchu pieszego w Japonii. W: Projektowa- nie, budowa i estetyka kładek dla pieszych. Ed. K. Flaga, W. Średniawa. Kraków: P. Krak. Wydz. Inż. Ląd. Kated. Bud. Mostów i Tuneli**2003 s. 165- 172, 14 rys. bibliogr. 1 poz. Cykl seminariów: Projektowanie, Budowa i Estetyka Kładek dla Pieszych. Kra- ków, 8 października, 5 listopada, 3 grudnia 2003 r.
PublikacjaOmówiono kilka przykładów budowli mostowych typowych dla dzisiejszej komuni-kacji pieszej w dużych miastach Japonii. rozważania obejmują kładki, galeriei pomosty - inaczej platformy komunikacyjne.
-
Reactive Processing and Functionalization of Ground Tire Rubber
PublikacjaThe dynamic development of the automotive industry resulted in a sig- nificant increase in rubber wastes, especially end-of-life tires, which are a serious threat to the natural environment and human health. This situation has enforced the industry and academic research groups to search new and cost-effective methods for recycling waste tires. In this field of research, reactive processing and functionaliza- tion seem to be a...
-
Dehydration converts DsbG crystal diffraction from low to high resolution
PublikacjaDiffraction quality crystals are essential for crystallographic studies of protein structure, and the production of poorly diffracting crystals is often regarded as a dead end in the process. Here we show a dramatic improvement of poorly diffracting DsbG crystals allowing high-resolution diffraction data measurement. Before dehydration, the crystals are fragile and the diffraction pattern is streaky, extending to 10 Å resolution....
-
Ancillary services to grids provided with distributed generation
PublikacjaThe paper introduces system for utilization of the customer-side generated energy. Additionally system can secure some ancillary services to grids. Those services include: current active filtering, load voltage stabilization and in case of grid voltage faults - uninterruptible load supplying (possible with energy storage device). Presented experimental results confirm flexibility of this arrangement in processes of power delivery...
-
In Search for Answers: Light4Health Online Course of Health Research for Interior Lighting Design
PublikacjaThree years ago, when I wrote an article in arc called: Human Centric Lighting. The New X Factor? (arc no. 108 Feb/Mar 2019), my intention was to raise questions in relation to the new topic and to find solid, research-based answers in the years to come. Today, I am happy to report that the lighting community, with the Light4Health Online Course of Health Research for Interior Lighting Design, has a useful tool, and consortium...
-
High Dynamic Range Microwave Displacement and Rotation Sensors Based on the Phase of Transmission in Groove Gap Waveguide Technology
PublikacjaThis research is focused on the design and realization of displacement sensors in gap waveguide technology. It is shown that with a small but fundamental change in the structure of a conventional gap waveguide, a linear displacement can be sensed. To this end, a unique feature of gap waveguides, i.e. the fact that no electrical connection between the top and bottom parts of the gap waveguide is required, is used. It is further shown...
-
The importance of a place in a small or medium - Sized Town.- Vol. 2
PublikacjaPublic space is a matter of concern for international humanitarian organisations. The paper describes the initiative of UNHabitat organisation for the creation of Charter of Public Space and the draft of this document. Next the definition of “place” is provided and the definitions of social capital and the bond between social capital and place attachment are presented. The actions proposed nowadays to create places are described....
-
The effect of chlorinated drinking water consumption on levels of volatile organohalogen compounds in urine.
PublikacjaW przedstawionej pracy podjęto próbę oznaczenia niezmienionych form lotnych rozpuszczalników chlorowcoorganicznych w próbkach materiału biologicznego jakim jest mocz dawców, używających w gospodarstwie domowym a także w pracy wody uzdatnianej między innymi w procesie chlorowania. Do wykonania oznaczeń zastosowano zmodyfikowaną technikę analizy fazy nadpowierzchniowej nad cienką warstwą cieczy z samoczynną generacją ciekłego sorbentu...
-
Wartość miejsca w małym i średnim mieście
PublikacjaPublic space is a matter of concern for international humanitarian organisations. The paper describes the initiative of UN-Habitat organisation for the creation of Charter of Public Space and the draft of this document. Next the definition of “place” is provided and the definitions of social capital and the bond between social capital and place attachment are presented. The actions proposed nowadays to create places are described....
-
KEYSTONE - JAKO PROCES AUTORYZACYJNY SYSTEMU OPENSTACK
PublikacjaNiniejszy artykuł ma na celu ukazanie funkcjonowania serwisu Keystone odpowiedzialnego za uwierzytelnianie użytkowników w architekturze OpenStack. Stanowi on front-end tego systemu, sprawując kontrolę nad dostępem do zasobów zapewnia scentralizowaną politykę bezpieczeństwa. Komunikacja z serwisem odbywa się na zasadzie klient-serwer, gdzie Keystone obsługuje żądania wysyłane do jego API, w zamian generując klucze dostępowe dla...
-
Pre-selection and assessment of green organic solvents by clustering chemometric tools
PublikacjaThe study presents the result of the application of chemometric tools for selection of physicochemical parameters of solvents for predicting missing variables – bioconcentration factors, water-octanol and octanol-air partitioning constants. EPI Suite software was successfully applied to predict missing values for solvents commonly considered as “green”. Values for logBCF, logKOW and logKOA were modelled for 43 rather nonpolar solvents...
-
Shaping of the turnout diverging track with variable curvature sections
PublikacjaThe paper presents an analytical method of shaping the turnout diverging railroad track with variable curvature segments on its length, which in an evident way distinguished it from a typical solution, made up of a single circular arc with no transition curves. Two separate causes including a linear and a nonlinear curvature one in the form of a polynomial have been examined. The obtained solutions of the problem have a universal...
-
Influence of an applied bearing system on behaviour of multi-span footbridge
PublikacjaThe cycle overpass in Gdynia is a box structure over 300 metres long, 10-span. It was opened for bicycle traffic in the second half of 2013. At the end of its construction, there was failure due to excessive horizontal displacement of the system. A number of bearings exceeded the range of permissible transverse shifts thus it was necessary to temporarily protect the spans from slipping. The FEM analysis of the original solution...
-
Cross-linking of epoxy-oligomeric mixtures in the presence of modified phenol-formaldehyde resins
PublikacjaCross-linking of epoxy oligomeric mixtures consisting of industrial epoxy resin ED-20, oligoetheracrylate TGM-3, peroxide derivative of epoxy resin ED-24 and phenol-formaldehyde oligomer with unsaturated methacrylate fragments or peroxide groups was studied in the presence of polyethylenepolyamine at room temperature and heated to 383, 403 or 423 K for 15, 30, 45, 60 and 75 minute. The influence of mixture composition, temperature...
-
The role of isolated farmsteads in the open landscape protection on the example of Kashubia
PublikacjaAs a result of the social and economic transformation of rural areas, open landscapes are disappearing. Former farmsteads are being devastated or beginning to lose their landscape context due to the spread of residential building development. At the same time, in many places, the farmstead form is clearly legible and remains an element with which the view is structured and enriched. The article was aimed at drawing attention to...
-
Application of gas chromatographic data and 2D molecular descriptors for accurate global mobility potential prediction
PublikacjaMobility is a key feature affecting the environmental fate, which is of particular importance in the case of persistent organic pollutants (POPs) and emerging pollutants (EPs). In this study, the global mobility classification artificial neural networks-based models employing GC retention times (RT) and 2D molecular descriptors were constructed and validated. The high usability of RT was confirmed based on the feature selection...
-
Enhancing Resilience of FSO Networks to Adverse Weather Conditions
PublikacjaOptical wireless networks realized by means of gigabit optical wireless communication (OWC) systems are becoming, in a variety of applications, an important alternative, or a complementary solution, to their fiber-based counterparts. However, performance of the OWC systems can be considerably degraded in periods of unfavorable weather conditions, such as heavy fog, which temporarily reduce the effective capacity of the network....
-
A Novel Cryptic Clostridial Peptide That Kills Bacteria by a Cell Membrane Permeabilization Mechanism
PublikacjaThis work reports detailed characteristics of the antimicrobial peptide Intestinalin (P30), which is derived from the LysC enzyme of Clostridium intestinale strain URNW. The peptide shows a broader antibacterial spectrum than the parental enzyme, showing potent antimicrobial activity against clinical strains of Gram-positive staphylococci and Gram-negative pathogens and causing between 3.04 ± 0.12 log kill for Pseudomonas aeruginosa...
-
Autonomous pick-and-place system based on multiple 3Dsensors and deep learning
PublikacjaGrasping objects and manipulating them is the main way the robot interacts with its environment. However, for robots to operate in a dynamic environment, a system for determining the gripping position for objects in the scene is also required. For this purpose, neural networks segmenting the point cloud are usually applied. However, training such networks is very complex and their results are unsatisfactory. Therefore, we propose...
-
Autonomous Perception and Grasp Generation Based on Multiple 3D Sensors and Deep Learning
PublikacjaGrasping objects and manipulating them is the main way the robot interacts with its environment. However, for robots to operate in a dynamic environment, a system for determining the gripping position for objects in the scene is also required. For this purpose, neural networks segmenting the point cloud are usually applied. However, training such networks is very complex and their results are unsatisfactory. Therefore, we propose...
-
Instantaneous impedance evaluation of dissolution of aisi 304 stainless steel during intergranular corrosion
PublikacjaCelem pracy było uzyskanie informacji dotyczących zróżnicowania parametrów elektrycznych uzyskanych na podstawie zarejestrowanych chwilowych widm impedancyjnych w zakresie aktywacji i reaktywacji roztwarzania wysokostopowej stali austenitycznej AISI podczas postępującego procesu korozji międzykrystalicznej. Badania prowadzono z wykorzystaniem dynamicznej elektrochemicznej spektroskopii impedancyjnej (DEIS). Pomiary wykonywano ''on-line''...
-
Atomic force microscopy based approach to local impedance measurements of grain interiors and grain boundaries of sensitized AISI 304 stanless steel
PublikacjaPraca prezentuje wyniki badań uzyskane z pomiarów techniką lokalnej spektroskopii impedancyjnej (LIS) wykonanych w trybie kontaktowym mikroskopii sił atomowych (AFM), które zostały przeprowadzone w lokalnych obszarach powierzchni ziarna austenitu i na granicy ziarna austenitu wysokostopowej stali austenitycznej AISI 304 poddanej procesowi uczulania oraz na próbkach odniesienia nie poddanych obróbce cieplnej.Badania LIS-AFM były...
-
Crystal and electronic structures of Ni(II) silanethiolates containing flexible diamine ligands
PublikacjaFive nickel(II) complexes containing aliphatic diamines and tri-tert-butoxysilanethiolate ligand have been synthesized for the purpose of studying their structural, spectral (IR, UV–Vis, HF EPR) and thermal properties. Complexes (1)–(5) have been prepared in high yield by reactions of [Ni{SSi(OtBu)3}2(NH3)(H2O)] with 1.3-propanediamine (L1), 1,6-hexanediamine (L2), or 1,7-heptanediamine (L3). The X-ray structures were determined...
-
Social Entrepreneurship Research – Challenges, Explanations and Suggestions for the Field Development
PublikacjaEmploying critical review and reflection on the key literature in the area of entrepreneurship and management studies in social entrepreneurship field, this paper aims to outline the current challenges this field is facing, while introducing related explanations and suggesting required changes. The current field of social entrepreneurship research is at its nascent stage, which is determined by ongoing definitional debates and...
-
Evaluation of adhesion strength, corrosion, and biological properties of the MWCNT/TiO2 coating intended for medical applications
PublikacjaMulti-wall carbon nanotube (MWCNT) coatings are gaining increasing interest because of their special properties used in many science fields. The titania coatings are known for their improvement of osteoblast adhesion, thus changing the surface architecture. Bi-layer coatings comprising 0.25 wt% of the MWCNTs and 0.30 wt% of titania (anatase structure) were synthesized in a two-stage procedure using the electrophoretic deposition...
-
DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation
PublikacjaA new diagnostic kit for K. oxytoca specific differentiation based on ddLMS PCR (ang. double digest ligation Mediated PCR) technique is shown. As a species-specific DNA fragment pehX gene, encoding the enzyme polygalactouronase, was chosen. The genome sequence of K. oxytoca is digested with two endonucleases: AclI and BclI which cut DNA before and after pehX gene. The polymorphic DNA fragments are ligated with AclI-end-specific...
-
Podeście do doskonalenia procesów w firmie informatycznej wspierane przez narzędzie Eclipse Process Framework Composer
PublikacjaArtykuł przedstawia podejście do doskonalenia procesów w mniejszych firmach informatycznych z wykorzystaniem narzędzia Eclipse Process Framework Composer. Podejście obejmuje modelowanie procesów wytwarzania oprogramowania w EPF Composer oraz wykorzystanie tych modeli do bieżącego zarządzania projektami w istniejących narzędziach wspomagających. Omówiono kroki wdrożenia rozwiązania, w tym sposób integracji modelu procesu z posiadanymi...
-
Automated detection of sleep apnea and hypopnea events based on robust airflow envelope tracking
PublikacjaThe paper presents a new approach to detection of apnea/hypopnea events, in the presence of artifacts and breathing irregularities, from a single-channel airflow record. The proposed algorithm identifies segments of signal affected by a high amplitude modulation corresponding to apnea/hypopnea events. It is shown that a robust airflow envelope—free of breathing artifacts—improves effectiveness of the diagnostic process and allows...
-
Outlet Flow Velocity In Circular Culvert
PublikacjaThe outlet flow velocity in the end section of the culvert barrel depends in most cases on the culvert geometry, including the barrel slope, as well as on upstream and downstream channel parameters. Flowing water can create pressure flow or free surface flow in the culvert barrel. In the case of an unsubmerged barrel outlet, the free-surface flow is more frequent than the full flow. Increased velocities can cause channel bed scour...
-
Implementation of TVDI calculation for coastal zone
PublikacjaPaper will show an implementation of TVDI (Temperature-Vegetation-Dryness Index) algorithm on GPU (Graphics Processing Unit). Calculation of this index is based on LST (Land Surface Temperature) and NDVI (Normalized Difference Vegetation Index). Discussed results are based on multi-spectral imagery retrieved from AVHRR3 sensors for area of Poland, especially from region of Gdańsk coastal zone. All phases of TVDI implementation...
-
Quaternion encryption methods for multimedia transmission, a survey of existing approaches
PublikacjaIn this paper we review quaternion encryption methods for multimedia transmission. We explain their weak and strong properties as well as suggest possible modifications. Our main focus is an algorithm QFC presented in paper by Dzwonkowski et al. (2015). All encryption methods, presented in this paper, use special properties of quaternions to perform rotations of data sequences in 3D space. Each method uses a common key generation...
-
Magnetic and capacitive couplings influence on power losses in double circuit high voltage overhead transmission line
PublikacjaPurpose – The paper aims to discuss problems of power and energy losses in a double-circuit overhead transmission line. It was observed from energymeters’ readings, that in such a line, active power losses can be measured as “negative”. The “negative” active power losses appear when the active power injected to the circuit is lower than the active power received at the circuit end. The purpose of this paper is to explain this phenomenon. Theoretical...
-
Adhesion of organic coatings to hot-dip galvanized steel
PublikacjaDuplex systems provide long-time corrosion protection, much longer than the sum of the lifetimes of zinc and paint used individually. However loss of adhesion between coating and zinc substrate is often found in practice. Different methods of zinc surface preparations are used in the fi eld. The aim of this study was to examine and compare coating protection when the old zinc surface, stored during long-time period (15 years) in...
-
ALIS-FLP: amplified ligation selected fragment-length polymorphism method for microbial genotyping
PublikacjaA DNA fingerprinting method known as ALIS-FLP (amplified ligation selected fragment-length polymorphism) has been developed for selective and specific amplification of restriction fragments from TspRI restriction endonuclease digested genomic DNA. The method is similar to AFLP, but differs in that only one specific restriction enzyme (TspRI) is used. The cohesive ends of the DNA fragments are ligated with two types of oligonucleotide....