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Sensors and System for Vehicle Navigation
PublikacjaIn recent years, vehicle navigation, in particular autonomous navigation, has been at the center of several major developments, both in civilian and defense applications. New technologies, such as multisensory data fusion, big data processing, or deep learning, are changing the quality of areas of applications, improving the sensors and systems used. Recently, the influence of artificial intelligence on sensor data processing and...
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Molecular dynamics study on the role of solvation water in the adsorption of hyperactive AFP to the ice surface
PublikacjaUsing computer simulations, the early stages of the adsorption of the CfAFP molecule to the ice surface were analyzed. We found that the ice and the protein interact at least as early as when the protein is about 1 nm away from the ice surface. These interactions are mediated by interfacial solvation water and are possible thanks to the structural ordering of the solvent. This ordering leads to positional preference of the protein...
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Low-power microwave-induced fabrication of functionalised few-layer black phosphorus electrodes: A novel route towards Haemophilus Influenzae pathogen biosensing devices
PublikacjaIn this paper, various passivation schemes were applied at few-layer black phosphorus (FLBP) to achieve covalent functionalisation with 4-azidobenzoic acid, improving its electrochemical response intended for analytical and biosensing applications. The thermal and microwave assisted modification procedures in toluene and dime-thylformamide resulted in high reversibility of reactions on functionalised FLBP using a ferricyanide/ferrocya-nide...
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Cyanogenic Compounds and Estrogen Disruptors
PublikacjaBalanced diet consists largely of plants containing cyanogenic compounds in the form of the more common cyanogenic glycosides and sometimes lipids. Maize, wheat, rye, apples, barley, oats, sugar cane and yet many other plants consumed by humans contain cyanogenic compounds. However the risk of poisoning is negligible as it is very easy to remove the toxic HCN by grinding and drying in air or soaking in water and an additional...
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In vitro antioxidative and binding properties of phenolics in traditional, citrus and exotic fruits
PublikacjaMany polyphenols bind proteins, therefore our research was focused on the potential of protein binding to polyphenols of investigated fruits and their health-related effects. The contents of polyphenols and related antioxidant activities of traditional, citrus and exotic fruits were compared. The presence of polyphenols (flavonoids and phenolic acids) in the investigated samples and their interaction with human serum albumin (HSA)...
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Impact of high-pressure processing on the bioactive compounds of milk - A comprehensive review
PublikacjaHigh-pressure processing (HPP) is a promising alternative to thermal pasteurization. Recent studies highlighted the effectivity of HPP (400–600 MPa and exposure times of 1–5 min) in reducing pathogenic microflora for up to 5 logs. Analysis of modern scientific sources has shown that pressure affects the main components of milk including fat globules, lactose, casein micelles. The behavior of whey proteins under HPP is very important...
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Iminodiacetate complex of cobalt(II) – Structure, physicochemical characteristics, biological properties and catalytic activity for 2-chloro-2-propen-1-ol oligomerization
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Monitoring the BTEX Volatiles during 3D Printing with Acrylonitrile Butadiene Styrene (ABS) Using Electronic Nose and Proton Transfer Reaction Mass Spectrometry
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Monitoring the BTEX Volatiles during 3D Printing with Acrylonitrile Butadiene Styrene (ABS) Using Electronic Nose and Proton Transfer Reaction Mass Spectrometry
PublikacjaWe describe a concept study in which the changes of concentration of benzene, toluene, ethylbenzene, and xylene (BTEX) compounds and styrene within a 3D printer enclosure during printing with different acrylonitrile butadiene styrene (ABS) filaments were monitored in real-time using a proton transfer reaction mass spectrometer and an electronic nose. The quantitative data on the concentration of the BTEX compounds, in particular...
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Intramolecular Proton Transfer in the Radical Anion of Cytidine Monophosphate Sheds Light on the Sensitivities of Dry vs Wet DNA to Electron Attachment-Induced Damage
PublikacjaSingle-strand breaks (SSBs) induced via electron attachment were previously observed in dry DNA under ultrahigh vacuum (UHV), while hydrated electrons were found not able to induce this DNA damage in an aqueous solution. To explain these findings, crossed electron-molecular beam (CEMB) and anion photoelectron spectroscopy (aPES) experiments coupled to density functional theory (DFT) modeling were used to demonstrate the fundamental...
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Evaluation of Terpene Decomposition in Kaffir Lime Juice during Storage Using Gas Chromatography–Mass Spectrometry and Proton Transfer Reaction–Mass Spectrometry
PublikacjaKaffir lime juice, often treated as production waste, can be a good source of terpenes. These compounds undergo various decomposition processes under the influence of external factors, especially during transportation and storage. In this paper, it was possible to monitor changes in the terpene profile of kaffir lime juice under different storage conditions, namely, 4 ◦C, 20 ◦C, and 35 ◦C. The identification of key decomposition...
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Występowanie oraz polimorfizm genów kodujących syntezę autotransporterów proteaz serynowych (SPATE) wśród bakterii Escherichia coli wywołujących biegunki u dzieci w regionie Gdańska
PublikacjaW grupie 96 szczepów bakterii Escherichia coli wyizolowanych w rejonie Gdańska od dzieci z biegunką zbadano obecność oraz polimorfizm sekwencji genów kodujących grupę białek o wspólnej nazwie: SPATE (Serine Protease Autotransporters of Enterobacteriaceae). Za pomocą analizy restrykcyjnej zidentyfikowano 8 różnych genów kodujących białka z tej grupy. Obecność jednego genu stwierdzono w 39 szczepach, w 15 szczepach występowały dwa,...
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The significance of the properties of water for the working cycle of the kinesin molecular motor
PublikacjaExplicit solvent molecular dynamics simulations were performed in this study to investigate and discuss several aspects of the influence of the properties of water on the working cycle of a molecular motor from the kinesin superfamily. The main objects of attention were: the binding of the neck linker and the association of the kinesin and the tubulin. The docking of the neck linker is considered a crucial event during the working...
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Highly Conserved Homotrimer Cavity Formed by the SARS-CoV-2 Spike Glycoprotein: A Novel Binding Site
PublikacjaAn important stage in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) life cycle is the binding of the spike (S) protein to the angiotensin converting enzyme-2 (ACE2) host cell receptor. Therefore, to explore conserved features in spike protein dynamics and to identify potentially novel regions for drugging, we measured spike protein variability derived from 791 viral genomes and studied its properties by molecular...
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EFFECTS OF DOPING ON STRUCTURE AND PHASE TRANSITION OF HIGH TEMPERATURE PROTON CONDUCTOR LaNb1-xMxO4-σ (M=As, Sb, V, Ta AND X=0,05 TO 0,3)
PublikacjaIn this work synthesis of lanthanum orthoniobate has been done. Doping by arsenic, antimony, vanadium or tantalum in 5% to 30% have been introduced. The relationship between doping type and percentage and structural properties of the material have been investigated.
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Induction of immune responses in sheep by vaccination with liposome-entrapped DNA complexes encoding Toxoplasma gondii MIC3 gene
PublikacjaToxoplasma gondii is a parasite that has been extensively studied due to its medical and veterinary importance in terminating pregnancies. Consequently, a satisfactory vaccine is required to control itsadverse effects on pregnant animals. The microneme protein, MIC3, is a major adhesion protein that binds to the surface of host cells and parasites, and is therefore a potential vaccine against T. gondii.The viability of MIC3 as...
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Amyloid fibril formation in the presence of water structure-affecting solutes
PublikacjaThe impact of the differently hydrated non-electrolytes (protein structure destabilizers) on the fibrillation of hen egg white lysozyme (HEWL) was investigated. Two isomeric urea derivatives i.e. butylurea (BU) and N,N,N′,N′-tetramethylurea (TMU) were chosen as a tested compounds. The obtained results show that butylurea exerts greater impact on HEWL and its fibrillation than tetramethylurea. Both substances decrease the time of...
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Asynchronous distributed state estimation for continuous-time stochastic processes
PublikacjaWe consider the problem of state estimation of a continuous-time stochastic process using an asynchronous distributed multi-sensor estimation system (ADES). In an ADES the state of a process of interest is estimated by a group of local estimators. Each local estimator based, for example, on a Kalman filter, performs single sensor filtration but also fusion of its local results and results from other (remote) processors to compute...
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Antifungal Effect of Penicillamine Due to the Selective Targeting of L-Homoserine O-Acetyltransferase
PublikacjaDue to the apparent similarity of fungal and mammalian metabolic pathways, the number of established antifungal targets is low, and the identification of novel ones is highly desirable. The results of our studies, presented in this work, indicate that the fungal biosynthetic pathway of L-methionine, an amino acid essential for humans, seems to be an attractive perspective. The MET2 gene from Candida albicans encoding L-homoserine...
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Pilicides effectively hinder chaperone-usher interactions in all Gram-negative bacteria
PublikacjaThe chaperone-usher pathway of adhesive structures biogenesis is highly conserved in all Gram-negative bacteria. Pili are polymers of thousands protein subunits that possess conserved immunoglobuline-like structure denoted by lack of the seventh G strand. The effect of this structural defect is a hydrophobic acceptor cleft. The folding of protein subunits is strictly dependent on the action of specific periplasmic chaperone protein...
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Polskie regulacje prawne, na tle przepisów unii europejskiej, dotyczące zawartości pestycydów w produktach żywnościowych=Polish regulations, comparing with European Union legislation, relating to pesticides content in foodstuffs
PublikacjaZanieczyszczenia żywności, m.in. pestycydy, występują w różnych produktach żywnościowych często w ilościach śladowych, stanowiąc mimo to poważne zagrożenie dla zdrowia człowieka. Prowadzone obecnie badania składu określonych produktów spożywczych i surowców rolniczych oraz występujących w nich zanieczyszczeń wykonywane na zlecenie producentów, importerów i eksporterów żywności, a także organizacji odpowiedzialnych za monitoring...
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Przeciwdziałanie procederowi prania pieniędzy na przykładzie wybranych regulacji prawnych Unii Europejskiej i Polski. Action against Money laundering as Exempliefied by selected legal Regulations of the European union and Poland
PublikacjaW opracowaniu omówiono zjawisko prania pieniędzy oraz jego negatywny wpływw na sktor finansowy i gospodarkę, ze szczególnym uwzględnieniem Polski i Unii Europejskiej.Krajowe regulacje bankowe w zakresie zwalczania tego procederu są w znacznym stopniu zgodne zarówno z regulacjami międzynarodowymi w tym FATF, jak i Unii Europejskiej.
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Suppressors of lapC mutation identify new regulators of LpxC, which mediates the first committed step in lipopolysaccharide biosynthesis
PublikacjaGram-negative bacteria, such as Escherichia coli, are characterized by an asymmetric outer membrane (OM) with lipopolysaccharide (LPS) located in the outer leaflet and phospholipids facing the inner leaflet. E. coli recruits LPS assembly proteins LapB, LapC and LapD in concert with FtsH protease to ensure a balanced biosynthesis of LPS and phospholipids. We recently reported that bacteria either lacking the periplasmic domain of...
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Application of human bile salts for in vitro digestion models
PublikacjaIn this study, experimental in vitro models simulating the environment of the human gastrointestinal tract were used to assess the impact of physiological surfactants, such as bile salts, on the kinetics of digestion. Bile salts are biosurfactants synthesised in the liver and secreted together with bile into the small intestine. There are many reports on the role of bile salts in lipolysis, but the knowledge of their influence...
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Sterowanie predykcyjne i fuzja danych w systemie dynamicznego pozycjonowania statku
PublikacjaRozprawa doktorska poświęcona jest badaniu zastosowania fuzji danych oraz sterowania predykcyjnego w systemie dynamicznego pozycjonowania statku. W pierwszej części pracy przedstawiono historię rozwoju systemów dynamicznego pozycjonowania, różne metody estymacji położenia statku, metody sterowania oraz cel i tezę pracy. Następnie zaprezentowano model matematyczny statku, kinematykę oraz dynamikę. W kolejnej części przedstawiono...
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Krajobraz nomadyczny w Muittalus samid birra (1910) Johana Turiego
PublikacjaThe article introduces Muittalus samid birra (1910, An account of the Sami) by Johan Turi, the fi rstbook with a secular content written in the sami language by a sami person. The author, a wolf hunterand reindeer-breeder, describes a broad spectrum of his people’s daily life. Pictures of everydayactivities are supplemented with stories about the sami people’s knowledge of the world (traditions,myths,...
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Automatic Incident Detection at Intersections with Use of Telematics
PublikacjaWhile there are many examples of Intelligent Transport System deployments in Poland, more attention should be paid to traffic incident management and detection on dual-carriageways and urban street networks. One of the aims of CIVITAS DYN@MO, a European Union funded project, is to use TRISTAR (an Urban Transport Management System) detection modules to detect incidents at junctions equipped with traffic signals. First part of paper...
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Analiza oddziaływań w kryształach związków zawierających w strukturze pierścień pirydynowy
PublikacjaMożliwość tworzenia się wiązań wodorowych jest kluczowym parametrem z punktu widzenia inżynierii kryształu, ponieważ wiązania wodorowe mają bezpośredni wpływ na strukturę oraz trwałość sieci krystalicznej. Głównym celem niniejszej rozprawy doktorskiej była analiza porównawcza wiązań wodorowych występujących w układach, zawierających w strukturze pierścień pirydynowy. Badane układy podzielono na: (1) układy ze związkami krzemosiarkowymi...
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Modelling of Objects Behaviour for Their Re-identification in Multi-camera Surveillance System Employing Particle Filters and Flow Graphs
PublikacjaAn extension of the re-identification method of modeling objects behavior in muti-camera surveillance systems, related to adding a particle filter to the decision-making algorithm is covered by the paper. A variety of tracking methods related to a single FOV (Field of Vision) are known, proven to be quite different for inter-camera tracking, especially in case of non-overlapping FOVs. The re-identification methods refer to the...
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Multibeam Echosounder and LiDAR in Process of 360-Degree Numerical Map Production for Restricted Waters with HydroDron
PublikacjaIn order to increase the safety of inland navigation and facilitate the monitoring of the coastal zone of restricted waters, a model of multi-sensory fusion of data from hydroacoustic and optoelectronic systems mounted on the autonomous survey vessel HydroDron will be developed. In the research will be used the LiDAR laser scanner and multibeam echosounder. To increase the visual quality and map accuracy, additionally side scan...
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Leveraging spatio-temporal features for joint deblurring and segmentation of instruments in dental video microscopy
PublikacjaIn dentistry, microscopes have become indispensable optical devices for high-quality treatment and micro-invasive surgery, especially in the field of endodontics. Recent machine vision advances enable more advanced, real-time applications including but not limited to dental video deblurring and workflow analysis through relevant metadata obtained by instrument motion trajectories. To this end, the proposed work addresses dental...
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Torsional elasticity and energetics of F1-ATPase
PublikacjaFoF1-ATPase is a rotary motor protein synthesizing ATP from ADP driven by a cross-membrane proton gradient. The proton flow through the membrane-embedded Fo generates the rotary torque that drives the rotation of the asymmetric shaft of F1. Mechanical energy of the rotating shaft is used by the F1 catalytic subunit to synthesize ATP. It was suggested that elastic power transmission with transient storage of energy in some compliant...
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Cloning, Expression and Purification of Wild-Type Trehalose Synthase from Deinococcus geothermalis
PublikacjaThe aim of this study was isolation and cloning of trehalose synthase gene derived from extremophilic microorganism to the expression vectors in the Tabor-Studier system and its expression in Rosetta(DE3)pLysS Escherichia coli cells. The second phase of the study consisted of proteins purification using an initial denaturation of host proteins and salting-out proteins by ammonium sulfate.
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Extracting functional groups of ALLINI to design derivatives of FDA‐approved drugs: Inhibition of HIV‐1 integrase
PublikacjaHIV‐1 integrase (IN) is crucial for integration of viral DNA into the host genome and a promising target in development of antiretroviral inhibitors. In this work, six new compounds were designed by linking the structures of two different class of HIV‐1 IN inhibitors (active site binders and allosteric IN inhibitors (ALLINIs)). Among newly designed compounds, INRAT10b was found most potent HIV‐1 IN inhibitor considering different...
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Correlation between the Reactivity and Spectroscopic Properties of N-Substituted Secondary Thioamides. New Intramolecular N• •H+• •N Binding Approach and Proton Complexes Based on Thioamide Ligation
PublikacjaFrom a comparison of chem. shifts and wavenumbers of several secondary thioamides and amides having monocationic substituents attached to thiocarbamoyl or carbamoyl groups by a polymethylene chain, new intramol. unconventional N···H+···N H bonding effects were discovered. It is argued that the CH2-N rotation is hindered and two +H···NHMe nonequiv. protons occur in a proton spectrum of hydrochloride RCH2C(S)NH(CH2)3NHMe-HCl...
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DNA-reactive anticancer imidazoacridinone C-1311 is a new inhibitor of hypoxia-inducible factor 1 alpha, vascular endothelial growth factor and tumor angiogenesis
PublikacjaHypoxia-inducible factor 1 (HIF-1) plays a critical role for tumor adaptation to hypoxia and promotes angiogenesis. Antitumor imidazoacridinone C-1311 is a DNA reactive topoisomerase II and FLT3 receptor tyrosine kinase inhibitor selected for phase II clinical trials for breast cancer. Here, we demonstrate the new mechanism of C-1311 action involving HIF-1a, vascular endothelial growth factor (VEGF) and angiogenesis as additional...
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Molecular Modeling of the Neurohypophyseal Receptor/Atosiban Complexes
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Molecular Dynamics Study of the Internal Water Molecules in Vasopressin and Oxytocin Receptors
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Therapy-Related Changes in the Serum Proteome Patterns of Early Stage Breast Cancer Patients with Different Outcomes
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Extracellular secretion of Pseudoalteromonas sp. cold-adapted esterase in Escherichia coli in the presence of Pseudoalteromonas sp. components of ABC transport system
PublikacjaEnzymy lipolityczne, głównie lipazy i esterazy, stanowią cenną grupę biokatalizatorów wykorzystywanych w przemyśle. Coraz większe zainteresowanie tą klasą enzymów sprawiło, że poszukuje się alternatywnych źródeł ich pozyskiwania. W celu zbadania wpływu białek transportu ABC Pseudoalteromonas sp. 643A na ekspresję genu, sekrecję i aktywność esterazy EstA z tego mikroorganizmu w systemie ekspresyjnym E. coli skonstruowano układ do...
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Novel method of expression and purification of hirudin based on pBAD TOPO, pTYB12 vectors and gene synthesis
PublikacjaW pracy przedstawiono nową, szybką i tanią metodę otrzymywania, ekspresji i oczyszczania białka hirudyny naturalnie izolowanego z ślinianek pijawki lekarskiej. Hirudyna pełni funkcje bezpośredniego inhibitora trombiny, głównego białka biorącego udział w tworzeniu skrzepów krwi. Właściwości te wykorzystywane są od wielu lat w klinice. Opracowana metoda pozwoliła uzyskać 17 mg/l hodowli E. coli białka o wysokiej czystości i aktywności...
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A two-plasmid Escherichia coli system for expression of Dr adhesins
PublikacjaPraca opisuje konstrukcję wydajnego systemu produkcji fimbrii Dr. System składa się z dwóch plazmidów. Plazmid pACYCpBAD-DraC-C-His zawiera pod kontrolą promotora arabinozowego gen draC kodujący białko kanałotwórcze DraC. Plazmid pET-30b-sygDraBE zawiera pod kontrolą promotora T7lac geny draB i draE kodujące odpowiednio białko opiekuńcze DraB oraz adhezynę DraE. Oba plzamidy posiadają różne ori replikacji co pozwala na ich jednoczesne...
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Synthesis and SAR Studies of Antibacterial Peptidyl Derivatives Based upon the Binding Site of Human Cystatin C
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Construction, purification, and functional characterization of His-tagged Candida albicans glucosamine-6-phosphate synthase expressed in Escherichia coli
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Purification to Homogeneity of Candida albicans Glucosamine-6-phosphate Synthase Overexpressed in Escherichia coli
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Analogues of Trypsin Inhibitor SFTI-1 with Disulfide Bridge Substituted by Various Length of Carbonyl Bridges
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Low-Molecular-Weight Aldehyde Inhibitors of Cathepsin G
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Pegylated Fluorescent Peptides as Substrates of Proteolytic Enzymes
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Designing of Substrates and Inhibitors of Bovine α-Chymotrypsin with Synthetic Phenylalanine Analogues in Position P1
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Molecular Simulations of Rhodopsin Tetrameter
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