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Search results for: PĘK RUR
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Use of MAG1 recombinant antigen for diagnosis of Toxoplasma gondii infection in humans
PublicationPraca opisuje klonowanie, oczyszczanie oraz zastosowanie w immunodiagnostyce toksoplazmozy antygenu rekombinantowego macierzy cyst tkankowych (MAG1) Toxoplasma gondii. Zastosowany system ekspresyjny pozwolił uzyskać dużą ilość rekombinantowego antygenu, który następnie wykorzystano w teście ELISA do wykrywania specyficzych przeciwciał anty-T. gondii klasy IgG w surowicach pacjentów chorych na toksoplazmozę. W przeprowadzonych badaniach...
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Enzymes of the lysine biosynthetic pathway as targets for antifungals ?
PublicationSystemic infections caused by human pathogenic fungi in immunocompromized patients continue to be one of the important clinical problems. Limited availability of safe and efficacious antifungal chemotherapeutics and emerging resistance to existing drugs stimulates search for novel molecular targets for antifungals. The α-aminoadipate pathway (AAP) of L-lysine biosynthesis is unique in fungi and thus has been so far considered...
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Phosphorylation of glucosamine-6-phosphate synthase is important but not essential for germination and mycelial growth of Candida albicans.
PublicationStosując mutagenezę ukierunkowaną dla genu gfa1 kodującego syntazę glukozamino-6-fosforanu Candida albicans otrzymano zmutowaną wersję GFA1S208A. Produkt zmutowanego genu, który utracił miejsce fosforylacji dla kinazy A, wykazywał wszystkie właściwości identyczne do dzikiego enzymu, jednakże nie był substratem dla kinazy. Brak fosforylacji syntazy glukozamino-6-fosforanu przez kinazęA prowadził do redukcji lecz nie eliminował...
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Identification and characterization of single-stranded-DNA-binding proteins from Thermus thermophilus and Thermus aquaticus - new arrangement of binding domains
PublicationOkreślono molekularne właściwości białek SSB-podobnych z Thermus thermophi-lus i T. aquaticus. Zbadano aktywność białek oraz termostabilność. Ponadto, dowiedziono homodimeryczność obu białek i ustalono minimalną sekwencję wiążącą ssDNA.
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Fatigue fracture morphology of AISI H13 steel obtained by additive manufacturing
PublicationThe paper focuses on researching the effect of fatigue loading on metallic structure, lifetime, and fracture surface topographies in AISI H13 steel specimens obtained by selective laser melting (SLM). The topography of the fracture surfaces was measured over their entire area, according to the entire total area method, with an optical three-dimensional surface measurement system. The fatigue results of the SLM 3D printed steel...
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Determination of Peak Impact Force for Buildings Exposed to Structural Pounding during Earthquakes
PublicationStructural pounding between adjacent, insufficiently separated buildings, or bridge segments, has been repeatedly observed during seismic excitations. Such earthquake-induced collisions may cause severe structural damage or even lead to the collapse of colliding structures. The aim of the present paper was to show the results of the study focused on determination of peak impact forces during collisions between buildings exposed...
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The construction of bifunctional fusion proteins consisting of MutS and GFP
PublicationSkonstruowano białka chimeryczne zawierające białko MutS z Thermus thermophilus i białko zielonej fluoresceiny GFP z Aequorea victoria, posiadające dla łatwiejszego oczyszczania domeny oligo-histydynowe na N- lub C-końcu. Fuzyjne białka rozpoznawały niekomplementarności w DNA podobnie do MutS T. thermophilus. Fluorescencyjne białka rozpoznające niekomplementarne DNA mogą być użyteczne w wykrywaniu jednonukleotydowych polimorfizmów...
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DAF - and collagen-binding properties of chimeric Dr fimbriae
PublicationEkspozycja białek heterologicznych na powierzchni mikroorganizmów jest jedną z nowych strategii stosowanych w mikrobiologii, biotechnologii oraz projektowaniu nowoczesnych szczepionek. W niniejszej pracy zbadano możliwość wykorzystania białka podjednostkowego DraE fimbrii Dr uropatogennych szczepów Escherichia coli jako nośnika heterologicznych sekwencji. W rejon odpowiadający N-terminalnej domenie 2 białka DraE wprowadzono epitopową...
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Propagation Characteristics of Partial Discharges in an Oil-Filled Power Transformer
PublicationPower transformers are among the most important assets in the power transmission and distribution grid. However, they suffer from degradation and possible faults causing major electrical and financial losses. Partial discharges (PDs) are used to identify the insulation health status and their degradation level. PDs are incipient, low-magnitude faults caused by localized dielectric breakdown. Those activities emit signals in many...
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The Effect of Protrusions on the Initiation of Partial Discharges in XLPE High Voltage Cables
PublicationThis paper is focusing on 3D Finite Elements Analysis (FEA) based modelling of protrusions as defects or imperfections in the XLPE high voltage cable. This study is aiming to examine the impact protrusions have on the initiation of partial discharges. Spherical and ellipsoidal protrusions with different sizes at the conductor screen of the high voltage cable is an essential content of this paper. In addition, a spherical gas-filled...
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Predictive current controller for sensorless induction motor drive
PublicationPrzedstawiono układ bezczujnikowego sterowania dla napedu z silnikiem klatkowym przy zastosowaniu predykcyjnego regulatora pradu. Zastosowany obserwator adaptacyjny wykazuje dużą odporność na zmiany parametrów silnika. W obserwatorze obliczane są wartości sił elektromotorycznych wykorzystywane w procesie regulacji predykcyjnej pradu silnika. Pokazano wyniki badań symulacyjnych i eksperymentalnych.
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Expression of a GDSL esterase from Pseudomonas sp. S9 in Pichiapastoris
PublicationCold active lipolytic enzymes are promising to replace the conventional enzymes processes of biotechnological industries. One of the most important feature of the cold-active lipases and esterases is that they offer economic benefits through energy saving. In general, they exhibit high activity at low temperatures and low thermostability at moderate temperatures. Lipolytic enzyme EstS9 was isolated from Pseudomonas sp. S9. A multiple...
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Analysis of the unique structural and physicochemical properties of the DraD/AfaD invasin in the context of its belonging to the family of chaperone/usher type fimbrial subunits
PublicationInwazyna DraD kodowana przez operon dra posiada strukturę charakterystyczną dla białek podjednostkowych fimbrii typu chaperone-usher. Fold-Ig białka DraD posiada dwie charakterystyczne cechy wyróżniające je z pośród rodziny fimbrialnych białek podjednostkowych: 1) zaburzenie struktury beta-beczki w rejonie szczeliny akceptorowej objawiające się układem wiązań wodorowych głównego łańcucha polipeptydowego oraz 2) nietypowo usytuowany...
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The construction of bifunctional fusion proteins consisting of MutS and GFP
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Assessment of the Feasibility of Energy Transformation Processes in European Union Member States
PublicationThe energy transition is now treated in most countries as a necessary condition for their long-term development. The process of energy transformation assumes the simultaneous implementation of the Sustainable Development Goals, which are a major challenge for modern economies and introduce significant restrictions in their functioning. Our study aims to group EU member states according to their ability to achieve energy transition...
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Bile conjugation and its effect on in vitro lipolysis of emulsions
PublicationBile Salts (BS) are responsible for stimulating lipid digestion in our organism. Gut microbiota are responsible for the deconjugation process of primary conjugated to secondary unconjugated BS. We use two structurally distinct BS and characterize the rate of lipolysis as a compound parameter. A static in-vitro digestion model as well as meta-analysis of literature data has been performed to determine the most influential factors...
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Service-based Resilience via Shared Protection in Mission-critical Embedded Networks
PublicationMission-critical networks, which for example can be found in autonomous cars and avionics, are complex systems with a multitude of interconnected embedded nodes and various service demands. Their resilience against failures and attacks is a crucial property and has to be already considered in their design phase. In this paper, we introduce a novel approach for optimal joint service allocation and routing, leveraging virtualized...
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Overcoming gender bias in the digital economy. Empirical evidence for European countries
PublicationThis study aims to contribute to understanding the gender biases that emerge in the digital technology-related field. More specifically we concentrate on examining whether gender gaps are diminishing or are persistent in terms of women’s enrollment in technology-related programs at the tertiary level of education and for female STEM graduates. Next, this evidence is confronted with gender biases in the labor market regarding changing...
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Model of optical phantoms thermal response upon irradiation with 975 nm dermatological laser
PublicationWe have developed a numerical model describing the optical and thermal behavior of optical tissue phantoms upon laser irradiation. According to our previous studies, the phantoms can be used as substitute of real skin from the optical, as well as thermal point of view. However, the thermal parameters are not entirely similar to those of real tissues thus there is a need to develop mathematical model, describing the thermal and...
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UNDECLARED WORK IN POLAND CHARACTERISTICS AND PREVALENCE
PublicationThis article aims to present the significance and prevalence of the phenomenon of undeclared work. First, we discuss the definitional issues of this phenomenon. Then, we present previous estimates on shadow economy and undeclared work in Poland, based on statistics provided by Central Statistical Office and other research entities. Our analysis covers the years 2000–2014. In the last part of the article, we use data from the Labour...
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Checkpointing of Parallel MPI Applications using MPI One-sided API with Support for Byte-addressable Non-volatile RAM
PublicationThe increasing size of computational clusters results in an increasing probability of failures, which in turn requires application checkpointing in order to survive those failures. Traditional checkpointing requires data to be copied from application memory into persistent storage medium, which increases application execution time as it is usually done in a separate step. In this paper we propose to use emerging byte-addressable...
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Czy na kanalizacji wód opadowych celowe jest stosowanie studzienki spadowej?
PublicationKanalizacja wód opadowych. Studzienka w sytuacji różnic rzędnych wlotu i wylotu. Specyfika pracy. Brak celowości studzienek spadowych z rurą.
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Quantum key distribution based on private states: Unconditional security over untrusted channels with zero quantum capacity
PublicationIn this paper, we prove unconditional security for a quantum key distribution (QKD) protocol based on distilling pbits (twisted ebits) from an arbitrary untrusted state that is claimed to contain distillable key. Our main result is that we can verify security using only public communication-via parameter estimation of the given untrusted state. The technique applies even to bound-entangled states, thus extending QKD to the regime...
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A graph coloring approach to scheduling of multiprocessor tasks on dedicated machines with availability constraints
PublicationWe address a generalization of the classical 1- and 2-processor unit execution time scheduling problem on dedicated machines. In our chromatic model of scheduling machines have non-simultaneous availability times and tasks have arbitrary release times and due dates. Also, the versatility of our approach makes it possible to generalize all known classical criteria of optimality. Under these stipulations we show that the problem...
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Synthesis and biological evaluation of fluorinated 3-phenylcoumarin-7-O-sulfamate derivatives as steroid sulfatase inhibitors
PublicationIn the present work, we report the initial results of our study on a series of 3-phenylcoumarin sulfamate-based compounds containing C-F bonds as a novel inhibitors of steroid sulfatase (STS). The new compounds are potent STS inhibitors, possessing more than 10 times higher inhibitory potency than coumarin-7-O-sulfamate. In the course of our investigation, compounds 2b and 2c demonstrated the highest inhibitory effect in the enzymatic...
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Wireless Charging Station Design for Electric Scooters: Case Study Analysis
PublicationThis study proposes an example of a wireless charging station design for a small-scale vehicle available on the market. The article analyzes basic transmitter inverter topologies and their compensation methods in terms of flexibility of control, tolerance to uncertainty in positioning, and the possibility of decreasing the integration price. Our comprehensive analysis focuses on the battery voltage range, energy capacity, cost,...
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Analysing By-Products Interaction as an Industry Resource of Circular Economy in Ukraine and the World
PublicationThe paper analyses existing and current scientific developments and literature sources, which show the advantages and disadvantages of many different influences of waste in Ukraine and other countries of Europe and the world. As a research result, stable connections have been established between the factors and criteria in assessing the by-product interaction as an industry resource. In our research, we used programs R.Studio and...
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Toward Intelligent Recommendations Using the Neural Knowledge DNA
PublicationIn this paper we propose a novel recommendation approach using past news click data and the Neural Knowledge DNA (NK-DNA). The Neural Knowledge DNA is a novel knowledge representation method designed to support discovering, storing, reusing, improving, and sharing knowledge among machines and computing systems. We examine our approach for news recommendation tasks on the MIND benchmark dataset. By taking advantages of NK-DNA, deep...
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Rotational Design Space Reduction for Cost-Efficient Multi-Objective Antenna Optimization
PublicationCost-efficient multi-objective design of antenna structures is presented. Our approach is based on design space reduction algorithm using auxiliary single-objective optimization runs and coordinate system rotation. The initial set of Pareto-optimal solutions is obtained by optimizing a response surface approximation model established in the reduced space using coarse-discretization EM simulation data. The optimization engine is...
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When Neural Networks Meet Decisional DNA: A Promising New Perspective for Knowledge Representation and Sharing
PublicationABSTRACT In this article, we introduce a novel concept combining neural network technology and Decisional DNA for knowledge representation and sharing. Instead of using traditional machine learning and knowledge discovery methods, this approach explores the way of knowledge extraction through deep learning processes based on a domain’s past decisional events captured by Decisional DNA. We compare our approach with kNN (k-nearest...
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Optimal Rotating Receiver Angles Estimation for Multicoil Dynamic Wireless Power Transfer
PublicationThis study proposed an approach to dynamic wireless charging that uses a rotating receiver coil. Our simulation study focused on the verification of a novel way of increasing the coupling coefficient and power transfer stability by following the flux of the transmitting coils. To obtain the highest possible coupling by means of the FEM analysis, we studied the optimization of the trajectory of the angular velocity of the rotating...
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Reliability Modeling for SPMD and DAC Applications in MERPSYS
PublicationIn this chapter we shall see a method for modeling of reliability of application execution in the MERPSYS system, and the results of reliability simulation of the same SPMD and DAC application which we have previously presented in this book. Unlike energy usage modeling and modeling of execution efficiency we could not compare the reliability model and simulation results to real experiment just because the experiment would last too...
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Synthesis and characterization of cycloaliphatic hydrophilic polyurethanes, modified with l-ascorbic acid, as materials for soft tissue regeneration
PublicationIn this paper we described synthesis and characteristic of obtained hydrophilic polyurethanes (PURs) modified with ascorbic acid (commonly known as vitamin C). Such materials may find an application in the biomedical field, for example in the regenerative medicine of soft tissues, according to ascorbic acid wide influence on tissue regeneration Flora (2009), Szymańska-Pasternak et al. (2011), Taikarimi and Ibrahim (2011), Myrvik...
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Otoczenie instytucjonalne a innowacyjność przedsiębiorstw
PublicationProblematyka innowacyjności w Polsce zajmuje wiele miejsca, zarówno w badaniach, raportach oraz w konstruowanych narzędziach wsparcia. Badania literaturowe wskazują na wagę instytucji w procesie przedsiębiorczym. Wykorzystując badania prowadzone wśród mikroprzedsiębiorstw w Polsce, przeanalizowano czynniki instytucjonalne, które mogą, według badań literaturowych, wpływać na innowacyjność tych podmiotów. Z badań wynika, że przedsiębiorcy...
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Linear-scaling calculation of Hartree-Fock exchange energy with Non-orthogonal Generalised Wannier Functions
PublicationWe present a method for the calculation of four-centre two-electron repulsion integrals in terms of localised non-orthogonal generalised Wannier functions (NGWFs). Our method has been imple- mented in the ONETEP program and is used to compute the Hartree-Fock exchange energy component of Hartree-Fock and Density Functional Theory (DFT) calculations with hybrid exchange-correlation functionals. As the NGWFs are optimised in situ...
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New technologies and diffusion of innovative financial products: Evidence on exchange-traded funds in selected emerging and developed economies
PublicationExchange-traded funds (ETFs) are one of the most rapidly-expanding categories of innovative financial products that have been introduced on many financial markets, in both emerging and developed economies. Our research contributes to the present state of knowledge by examining factors, including information and communication technologies (ICTs), influencing the diffusion of ETFs. In our research, we consider also the impact of...
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Application of Text Analytics in Public Service Co-Creation: Literature Review and Research Framework
PublicationThe public sector faces several challenges, such as a number of external and internal demands for change, citizens' dissatisfaction and frustration with public sector organizations, that need to be addressed. An alternative to the traditional top-down development of public services is co-creation of public services. Co-creation promotes collaboration between stakeholders with the aim to create better public services and achieve...
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A novel architecture for e-learning knowledge assessment systems
PublicationIn this paper we propose a novel e-learning system, dedicated strictly to knowledge assessment tasks. In its functioning it utilizes web-based technologies, but its design differs radically from currently popular e-learning solutions which rely mostly on thin-client architecture. Our research proved that such architecture, while well suited for didactic content distribution systems is ill-suited for knowledge assessment products....
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Bio-inspired Decisional DNA in Machinas and other Man-made Systems: The Way Forward
PublicationArtificial bio-inspired intelligent techniques and systems supporting smart, knowledge-based solutions of real world problems which are currently researched very extensively by research teams around the world, have enormous potential to enhance automation of decision making and problem solving for a number of diverse areas including design, manufacturing, Information Technology (IT), social communities of practice, and economics...
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A novel architecture for e-learning knowledge assessment systems
PublicationIn this paper we propose a novel e-learning system, dedicated strictly to knowledge assessment tasks. In its functioning it utilizes web-based technologies, but its design differs radically from currently popular e-learning solutions which rely mostly on thin-client architecture. Our research proved that such architecture, while well suited for didactic content distribution systems is ill-suited for knowledge assessment products....
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Society 4.0: Issues, Challenges, Approaches, and Enabling Technologies
PublicationThis guest edition of Cybernetics and Systems is a broadening continuation of our last year edition titled “Intelligence Augmentation and Amplification: Approaches, Tools, and Case Studies”. This time we cover research perspective extending towards what is known as Society 4.0. Bob de Vit brought the concept of Society 4.0 to life in his book “Society 4.0 – resolving eight key issues to build a citizens society”. From the Systems...
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A novel architecture for e-learning knowledge assessment systems
PublicationAbstract. In this paper we propose a novel e-learning system, dedicated strictly to knowledge assessment tasks. In its functioning it utilizes web-based technologies, but its design differs radically from currently popular e-learning solutions which rely mostly on thin-client architecture. Our research proved that such architecture,while well suited for didactic content distribution systems is ill-suited for knowledge assessment...
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Compact global association based adaptive routing framework for personnel behavior understanding
PublicationPersonnel behavior understanding under complex scenarios is a challenging task for computer vision. This paper proposes a novel Compact model, which we refer to as CGARPN that incorporates with Global Association relevance and Adaptive Routing Pose estimation Network. Our framework firstly introduces CGAN backbone to facilitate the feature representation by compressing the kernel parameter space compared with typical algorithms,...
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Bees Detection on Images: Study of Different Color Models for Neural Networks
PublicationThis paper presents an approach to bee detection in video streams using a neural network classifier. We describe the motivation for our research and the methodology of data acquisition. The main contribution to this work is a comparison of different color models used as an input format for a feedforward convolutional architecture applied to bee detection. The detection process has is based on a neural binary classifier that classifies...
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Evaluation and behaviour of slowly biodegradable substrate on mathematical modelling of oxygen uptake rate in activated sludge systems
PublicationThe effect of the readily biodegradable (Ss) fraction in activated sludge systems has been extensively investigated, but only minor information can be found about the effect of Xs on OUR in activated sludge systems. The aim of this study was to determine the evaluation and behaviour of Xs on mathematical modelling of OUR using ASM2d and its modification. The essential study was divided into two major parts: experimental investigation...
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A highly-efficient technique for evaluating bond-orientational order parameters
PublicationWe propose a novel, highly-efficient approach for the evaluation of bond-orientational order parameters (BOPs). Our approach exploits the properties of spherical harmonics and Wigner 3jj-symbols to reduce the number of terms in the expressions for BOPs, and employs simultaneous interpolation of normalised associated Legendre polynomials and trigonometric functions to dramatically reduce the total number of arithmetic operations....
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Model-Driven Testing of Real-Time Embedded Systems - From Object Oriented towards Function Oriented Development
PublicationMBD
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Transformational Leadership and Acceptance of Mistakes as a Source of Learning: Poland-USA Cross-Country Study
PublicationThis study explores the influence of transformational leadership on internal innovativeness mediated by mistakes acceptance, including country and industry as factors to be considered and gender and risk-taking attitude as moderators. General findings, primarily based on the US samples (healthcare, construction, and IT industry), confirmed that transformational leadership and internal innovativeness are mediated by mistakes acceptance...
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Path integrals formulations leading to propagator evaluation for coupled linear physics in large geometric models
PublicationReformulating linear physics using second kind Fredholm equations is very standard practice. One of the straightforward consequences is that the resulting integrals can be expanded (when the Neumann expansion converges) and probabilized, leading to path statistics and Monte Carlo estimations. An essential feature of these algorithms is that they also allow to estimate propagators for all types of sources, including initial conditions....
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Modeling protein structures with the coarse-grained UNRES force field in the CASP14 experiment
PublicationThe UNited RESidue (UNRES) force field was tested in the 14th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP14), in which larger oligomeric and multimeric targets were present compared to previous editions. Three prediction modes were tested (i) ab initio (the UNRES group), (ii) contact-assisted (the UNRES- contact group), and (iii) template-assisted (the UNRES-template...