Wyniki wyszukiwania dla: COLD-ADAPTED ENZYME
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Antifungal Effect of Penicillamine Due to the Selective Targeting of L-Homoserine O-Acetyltransferase
PublikacjaDue to the apparent similarity of fungal and mammalian metabolic pathways, the number of established antifungal targets is low, and the identification of novel ones is highly desirable. The results of our studies, presented in this work, indicate that the fungal biosynthetic pathway of L-methionine, an amino acid essential for humans, seems to be an attractive perspective. The MET2 gene from Candida albicans encoding L-homoserine...
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Overproduction of CaMet15p native and His-tag versions.
Dane BadawczeEnzyme of fungal L-methionine biosynthetic pathway: O-acetylhomoserine sulfhydrylase (Met15p) could be exploited as molecular target for antifungal chemotherapy. The goal of the study was to obtain conditions optimal for protein production with the use of prokaryotic cells. The constructed expression plasmids, designed in three different versions: encoding...
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The electrochemical studies of CB-PLA electrode after surface activation by proteinase K digestion
Dane BadawczeThe dataset contains the electrochemical studies performed to evaluate surface activation of the CB-PLA 3D printed electrodes by enzymatic hydrolysis in a solution containing proteinase K (72h digestion period). Different enzyme concentrations were evaluated: 0.2, 0.4, 0.6 and 0.8 mg/ml. The studies were performed before surface activation, during and...
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Overproduction of CaStr2p native and His-tag versions.
Dane BadawczeEnzyme of fungal L-methionine biosynthetic pathway: cystathionine-γ-synthase (CaStr2p) could be exploited as molecular target for antifungal chemotherapy. The goal of the study was to obtain conditions optimal for protein production with the use of prokaryotic cells. The constructed expression plasmids, designed in three different versions: encoding...
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Overproduction of homoserine O-acetyltransferase (CaMet2p) native and His-tag versions.
Dane BadawczeEnzyme of fungal L-methionine biosynthetic pathway: homoserine O-acetyltransferase (Met2p) could be exploited as molecular target for antifungal chemotherapy. The goal of the study was to obtain conditions optimal for protein production with the use of prokaryotic cells. The constructed expression plasmids, designed in three different versions: encoding...
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The generalization by simplification operator with Chrobak’s method of objects representing groups of buildings in Kartuzy district - scale 1:10000
Dane BadawczeThe process of automatic generalization is one of the elements of spatial data preparation for the purpose of creating digital cartographic studies. The presented data include a part of the process of generalization of building groups obtained from the national geodesy and cartography resource from BDOT10k (10k topographic database) [1].
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The generalization by simplification operator with Chrobak’s method of objects representing groups of buildings in Gdańsk district - scale 1:10000
Dane BadawczeThe process of automatic generalization is one of the elements of spatial data preparation for the purpose of creating digital cartographic studies. The presented data include a part of the process of generalization of building groups obtained from the national geodesy and cartography resource from BDOT10k (10k topographic database) [1].
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The generalization by simplification operator with Chrobak’s method of objects representing groups of buildings in Katowice district - scale 1:10000
Dane BadawczeThe process of automatic generalization is one of the elements of spatial data preparation for the purpose of creating digital cartographic studies. The presented data include a part of the process of generalization of building groups obtained from the national geodesy and cartography resource from BDOT10k (10k topographic database) [1].
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The generalization by simplification operator with Chrobak’s method of objects representing groups of buildings in Katowice district - scale 1:25000
Dane BadawczeThe process of automatic generalization is one of the elements of spatial data preparation for the purpose of creating digital cartographic studies. The presented data include a part of the process of generalization of building groups obtained from the national geodesy and cartography resource from BDOT10k (10k topographic database) [1].
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The generalization by simplification operator with Chrobak’s method of objects representing groups of buildings in Gdańsk district - scale 1:25000
Dane BadawczeThe process of automatic generalization is one of the elements of spatial data preparation for the purpose of creating digital cartographic studies. The presented data include a part of the process of generalization of building groups obtained from the national geodesy and cartography resource from BDOT10k (10k topographic database) [1].
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The generalization by simplification operator with Chrobak’s method of objects representing groups of buildings in Kartuzy district - scale 1:25000
Dane BadawczeThe process of automatic generalization is one of the elements of spatial data preparation for the purpose of creating digital cartographic studies. The presented data include a part of the process of generalization of building groups obtained from the national geodesy and cartography resource from BDOT10k (10k topographic database) [1].
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DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation
PublikacjaA new diagnostic kit for K. oxytoca specific differentiation based on ddLMS PCR (ang. double digest ligation Mediated PCR) technique is shown. As a species-specific DNA fragment pehX gene, encoding the enzyme polygalactouronase, was chosen. The genome sequence of K. oxytoca is digested with two endonucleases: AclI and BclI which cut DNA before and after pehX gene. The polymorphic DNA fragments are ligated with AclI-end-specific...
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Septic safe interactions with smart glasses in health care
PublikacjaIn this paper, septic safe methods of interaction with smart glasses, due to the health care environment applications consideration, are presented. The main focus is on capabilities of an optical, proximity-based gesture sensor and eye-tracker input systems. The design of both interfaces is being adapted to the open smart glasses platform that is being developed under the eGlasses project. Preliminary results obtained from the...
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Bunker demolition located in a conservation protection zone in the light of technical, national defense, functional-utility and economic conditions
PublikacjaThe paper presents a description of the technical condition of the Bunker building located at the port quay, which has been out of use for several decades. The direct reason for the cessation of exploitation was the deletion of Bunker from the Civil Defense records. The paper contains a detailed analysis of the Bunker's technical condition, also taking into account the national defense, functional and utility aspects and the issues...
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Gender differences in the perception of the Quality of College Life in Spanish University
PublikacjaThrough the current research, we intend to analyse how students differ in their levels of quality of college life (QCL) according to gender, and how this relates to overall quality of life (QoL) and loyalty [measured by Identification and word of mouth (WoM)] to their specific university. The survey included 243 students attending public university in Spain. The results obtained through the analysis of data allow affirming that...
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WSPÓŁPRACA BLOKU GAZOWO-PAROWEGO Z OBIEGIEM ORC = Combinded power plant in cooperation with ORC
PublikacjaIn the paper presented is the idea of a combined gas and steam power plant cooperation with a low temperature organic Rankine cycle ORC unit. Considerations presented in the paper are accomplished for the Gorzów CHP. The intention of present analysis is heat recovery of low temperature energy and its further use in ORC cycle in order to produce electricity, without increase in fuel consumption. Waste energy is recovered from the...
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TRUSS JOINT WITH POSITIVE ECCENTRICITY EXPERIMENTAL RESEARCH
PublikacjaDue to the technological reasons in modern lightweight steel trusses, fabricated from cold- formed sections, positive eccentricities appear in the truss nodes what induce additional forces in the truss chords. To account for the real load-carrying capacity of truss node area the steel structure research in scale 1:1 were conducted. The experiments consisted of two parts: preliminary and proper one, when conclusions from...
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Ocena kosztu cyklu życia szyn kolejowych w modernizacji układów geometrycznych
PublikacjaW pracy przedstawiono uwarunkowania historyczne, które doprowadziły do opracowania metod szacowania kosztu życia inwestycji. Opisano podstawowe założenia LCC według modelu przedstawionego w Normach oraz opracowanego w projekcie INNOTRACK. Zaproponowano połączenie powyższych metod i dostosowanie ich do oceny kosztu życia szyn kolejowych w Polce. Przedstawiono elementy wpływające na koszt utrzymania nawierzchni szynowej oraz metody...
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Amino Acid Based Antimicrobial Agents – Synthesis and Properties
PublikacjaStructures of several dozen of known antibacterial, antifungal or antiprotozoal agents are based on the amino acid scaffold. In most of them, the amino acid skeleton is of a crucial importance for their antimicrobial activity, since very often they are structural analogs of amino acid intermediates of different microbial biosynthetic pathways. Particularly, some aminophosphonate or aminoboronate analogs of protein amino acids are...
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Characterization of recombinant homocitrate synthase from Candida albicans
PublikacjaLYS21 and LYS22 genes from Candida albicans encoding isoforms of homocitrate synthase (HCS), an enzyme catalyzing the first committed step in the L-lysine biosynthetic pathway, were cloned and expressed as NoligoHistagged fusion proteins in Escherichia coli. The purified gene products revealed HCS activity, i.e. catalyzed the condensation of α-ketoglutarate with acetyl-coenzyme A to yield homocitrate. The recombinant enzymes were purified...
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Classification of Music Genres Based on Music Separation into Harmonic and Drum Components . Klasyfikacja gatunków muzycznych wykorzystująca separację instrumentów muzycznych
PublikacjaThis article presents a study on music genre classification based on music separation into harmonic and drum components. For this purpose, audio signal separation is executed to extend the overall vector of parameters by new descriptors extracted from harmonic and/or drum music content. The study is performed using the ISMIS database of music files represented by vectors of parameters containing music features. The Support Vector...
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Analysis of EN 1993-1-6 guidelines about determining amplitudes of equivalent imperfections of steel cylindrical shells subjected to uniform external pressure
PublikacjaCivil engineering structures should be designed with reference to relevant standards. One of them is a Eurocode 3 standard EN 1993-1-6:2007: Design of steel structures Part 1-6: Strength and Stability of Shell Structures. According to this standard, the value of buckling load can be determined using different approaches: classical hand calculations (stress design) and geometrical and material non-linear analysis of an imperfect...
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Heat transfer in plate heat exchanger channels: experimental validation of selected correlation equations
PublikacjaThis study is focused on experimental investigation of selected type of brazed plate heat exchanger. The Wilson approach was applied in order to estimate heat transfer coefficients for the PHEx passages. The main aim of the paper was to experimentally check ability of several correlations published in the literature to predict heat transfer coefficients by comparison experimentally obtained data with appropriate predictions. The...
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Heat transfer in plate heat exchanger channels: experimental validation of selected correlation equations
PublikacjaThis study is focused on experimental investigation of selected type of brazed plate heat exchanger. The Wilson approach was applied in order to estimate heat transfer coefficients for the PHEx passages. The main aim of the paper was to experimentally check ability of several correlations published in the literature to predict heat transfer coefficients by comparison experimentally obtained data with appropriate predictions. The...
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A new division of bacterial UvrA homologues
PublikacjaThe UvrA protein is a DNA-binding and damage-recognition enzyme which participates in the prokaryotic type nucleotide excision repair (NER) pathway. It has recently been noted that some bacterial genomes comprise additional uvrA genes which encode five distinct types of UvrA homologue. We investigated the sequences of over 2400 bacterial genomes and found 130 examples of bacteria containing uvrA 2 genes. The sequence analyses conducted...
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Polysaccharides-Based Hybrid Materials for Bio- and Non-Bio Sectors. Edytorial
PublikacjaThis special edition spotlights the recent research in the design, development, and emerging applications of polysaccharides-based hybrid materials for biotechnological and biomedical purposes. All the articles published in this issue underscore the significance of materials derived from cellulose, alginate, chitosan, starch, and carrageenan for various applications, including enzyme production, encapsulation, targeted drug delivery,...
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New potent STS inhibitors based on fluorinated 4-(1-phenyl-1H-[1,2,3]triazol-4-yl)-phenyl sulfamates
PublikacjaA series of fluorinated analogs based on the frameworks of 4-(1- phenyl-1H-[1,2,3]triazol-4-yl)-phenyl sulfamates have been synthesized as steroid sulfatase (STS) inhibitors. The design of chemical structures of new potential STS inhibitors was supported by molecular docking techniques to identify potential interactions between inhibitors and amino acid residues located in the STS active site. The STS inhibitory potency was evaluated...
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Optimization of the femtosecond laser impulse for excitation and the spin-orbit-mediated dissociation in the NaRb molecule
Dane BadawczeHigh accuracy ab initio potential energy curves (1tSigma+, 2sSigma+, 1tPi), electronic transition dipole moment function (1tSigma+ - 1tPi), and spin-orbit coupling (2sSigma+ - 1tPi) have been calculated for the NaRb molecule. The time-dependent excitation and dissociation processes in the polar alkali diatomic NaRb molecule and the quantum properties...
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D.C. electrical measurements of Bi2VO5.5 ceramic measured at high temperature region
Dane BadawczeD.C. electrical properties of Bi2VO5.5 ceramic was measured.
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Simple and low-cost wireless voting system
PublikacjaThis paper presents the concept of a simple and low-cost wireless voting system working in the 868 MHz frequency band. The described system is dedicated to general shareholders assemblies but it can be easily adapted for other applications. The main advantage is its simplicity and mobility as it consists solely of three components - voting modules, a base station and a PC application from which the whole system is mamaged. This...
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Simple and low-cost wireless voting system
PublikacjaThis paper presents the concept of a simple and low-cost wireless voting system working on the 868 MHz frequency band. Described system is dedicated to general shareholders assemblies but it can be easily adapted for other applications. The main advantage is its simplicity and mobility as it consists solely of three components - voting modules, base station and a PC application from which the whole system is managed. This architecture...
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Buckling of thin-walled columns accounting for initial geometrical imperfections
PublikacjaThe paper is devoted to the effect of some geometrical imperfections on the critical buckling load of axially compressed thin-walled I-columns. The analytical formulas for the critical torsional and flexural buckling loads accounting for the initial curvature of the column axis or the twist angle respectively are derived. The classical assumptions of theory of thin-walled beams with non-deformable cross-sections are adopted. The...
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Demolition of the cereal elevator building located in the conservation protection zone in the aspect of technical, functional-utility and economic conditions
PublikacjaThe paper presents a description of the technical condition of the Grain Elevator building situated on the port quay, which after many years of operation has been decommissioned. The immediate reason for the cessation of operation was the change in the use profile of the port quay, which caused the handling of other goods at the quay than the bulk materials originally stored in Elewator Zbożowy. The work contains...
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Polyurethanes as a Potential Medical-Grade Filament for Use in Fused Deposition Modeling 3D Printers – a Brief Review
PublikacjaThe possibility of using 3D printing technology (3DP) in medical field is a kind of revolution in health care. This has contributed to a rapid growth in demand for 3D printers, whose systems and materials are adapted to strict medical requirements. In this paper, we report a brief review of polyurethanes as a potential medical-grade filament for use in Fused Deposition Modeling (FDM) 3D printer technology. The advantages of polyurethanes...
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Analytical design method of reverse curves
PublikacjaThe paper deals with a new approach relating to the design of the region of railway track direction alteration adapted to Mobile Satellite Measurements technique. The method may be particularly useful in situations when both the straights of the route directions cannot be connected in an elementary way using a circular arc with transition curves; this also refers to the application of a compound curve. Thus the only solution becomes...
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A Measurement System for Quasi-Spectral Determination of Absorption and Scattering Parameters of Veterinary Tissue Phantoms
PublikacjaThis paper describes the construction of a system for the quasi-spectral determination of absorption and scattering parameters of animal tissue phantoms. Several tissue phantoms, including one reference and two modified for examination, were prepared from polydimethylsiloxane (PDMS). The phantoms were measured using a system based on an integrating sphere and the light sources of wavelengths commonly used for the treatment of various...
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3D polypyrrole structures as a sensing material for glucose detection
PublikacjaIn this work, 3D polypyrrole (PPy) structures as material for glucose detection is proposed. Polypyrrole was electrochemically polymerized on platinum screen-printed electrode from an aqueous solution of lithium perchlorate and pyrrole. The growth mechanism of such PPy structures was studied by ex-situ scanning electron microscopy. Preliminary studies show that studied here PPy film is a good candidate as a sensing material for...
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Molecular docking studies towards development of novel Gly-Phe analogs for potential inhibition of Cathepsin C (dipeptidyl peptidase I).
PublikacjaCathepsin C is a cysteine protease required for activation of various pro-inflammatory serine proteases and, essentially, is of interest as a therapeutic target. Cathepsin C coordinate system was employed as a model to study the interaction of some already available inhibitors of Cathepsin C. Compounds containing Gly-Phe fragment with functional groups at its ends were designed by knowledge based approach. Using AutoDock and...
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PCR-ELISA: inexpensive alternative to quantitative PCR
PublikacjaPCR-ELISA (polymerase chain reaction-enzyme linked immunosorbent assay), a combination of PCR and ELISA methods, has been used since late 1980s. The technique is based on specially labelled DNA fragments which are captured by specific DNA sequences and detected by antibodies. The whole procedure of PCR-ELISA is divided into three steps: DNA extraction, PCR reaction and detection by ELISA. The method has been found as very specific...
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Hybrid battery based on arylated carbon nanotubes and laccase
PublikacjaSingle-walled carbon nanotubes (SWCNT) were covalently modified with anthracene and anthraquinone and used for the construction of cathodes for biocatalytic reduction of dioxygen. The nanotubes with aromatic groups casted onto the electrode increased the working surface of the electrode and enabled efficient direct electron transfer (DET) between the enzyme and the electrode. The aryl groups enter the hydrophobic pocket of the...
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Pharmacomicrobiomics of cell-cycle specific anti-cancer drugs – is it a new perspective for personalized treatment of cancer patients?
PublikacjaIntestinal bacteria are equipped with an enzyme apparatus that is involved in the active biotrans-formation of xenobiotics, including drugs. Pharmacomicrobiomics, a new area of pharmacology, analyses interactions between bacteria and xenobiotics. However, there is another side to the coin. Pharmacotherapeutic agents can significantly modify the microbiota, which consequently affects their efficacy. In this review, we comprehensively...
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PCR-RFLP assays for species-specific identification of fungi belonging to Scopulariopsis and related genera
PublikacjaFungi of the Scopulariopsis genus, commonly found in the environment, are opportunistic pathogens that can cause various types of human infections. So far, no efficient molecular method has been developed for species differentiation among Scopulariopsis and related genera. In order to advance this field, we have evaluated performance of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assays, based...
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IKE1-IKE3 (C-1305 derivatives) inhibitory effect of the Yeast Topoisomerase II relaxation activity
Dane BadawczeInhibition of Yeast Topoisomerase II were analyzed according to relaxation assay kit from Inspiralis. Briefly, 250 ng of supercoiled pBR322 DNA, 1 mM ATP, 1-200 μM of analyzed compound were mixed with reaction buffer (1 mM Tris-HCl (pH 7.9), 10 mM KCl, 0.5 mM MgCl 2, 0.2 % (v/v) glycerol). The reaction was initiated by the addition of an enzyme, allowed...
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Effect of antitumor compounds on the yeast topoisomerase II relaxation activity
Dane BadawczeThe datasets contain results of antitumor compounds* (known inhibitors of human topoisomerase alpha II) inhibitory activity against yeast topoisomerase II. DNA topoisomerases (Topo) are enzymes that catalyze changes in the spatial structure of DNA and play an important role in replication, transcription and recombination. Beyond their normal functions,...
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Underwater in situ local heat treatment by additional stitches for improving the weldability of steel
PublikacjaIn this paper the influence of in situ local heat treatment performed by additional stitches on the weldability of high-strength low-alloy (HSLA) S355J2C+N steel was tested. The investigated steel is characterized by high susceptibility to cold cracking. It is necessary to find a method to improve the quality of welded joints. The local heat treatment was applied as an eect of bead-on plate welding made on the face of a Tekken...
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Wystawa poświęcona prof. Wacławowi Szybalskiemu
WydarzeniaWystawa przygotowana przez Fundację Profesora Wacława Szybalskiego.
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THE CASE OF MANAGEMENT CONCEPTS IN CIVIL AVIATION BUSINESS.
PublikacjaThe article presents the changes in management concepts used in civil aviation business in last 25 years. There is a need to make prognosis and support decision-making processes with different models but each model is adapted to current management concept. This article will present the changes and possible future trends in management concepts with the description of strengths and weaknesses of each of them. These might be used...
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Current based Normalized Triple Covariance as a bearings diagnostic feature in induction motor
PublikacjaDiagnosis of induction motors, conducted remotely by measuring and analyzing the supply current is attractive with the lack of access to the engine. So far there is no solution, based on analysis of current, the credibility of which allow use in industry. Statistics of IM bearing failures of induction motors indicate, that they constitute more than 40% of IM damage, therefore bearing diagnosis is so important. The article provides...
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Current-based higher-order spectral covariance as a bearing diagnostic feature for induction motors
PublikacjaConducting the diagnosis of induction motors remotely by analyzing the supply current is an attractive prospect with the lack of access to the engine. Currently, there is no solution, based on analysis of the current, the credibility of which would allow its use in industry. The statistics of bearing failures in induction motors indicate that they constitute more than 40% of induction motor damage, therefore, bearing diagnosis...
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AUTOMATIC LEARNING OF STRATEGY AND RULES IN CARD GAMES USING IMAGE FROM CAMERA
PublikacjaBelow work tries to answer a question: if it is possible to replace real human with computer system in social games. As a subject for experiments, card games were chosen, because they require a lot of player interaction (playing and taking cards), while their rules are easy to present in form of clear list of statements. Such a system, should allow real players to play without constant worrying about guiding or helping computer...